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| 1 | Efficient generation of hepatocyte-like cells from human induced pluripotent stem cells显示文摘人的导致的 pluripotent 茎(iPS ) 房间类似于胚胎的茎(ES ) 房间,和罐头强烈地增殖并且区分进许多房间类型。然而,人的 iPS 房间的肝的区别还没被报导了。在这份报告,人的 iPS 房间被导致由一个逐步的协议区分进肝的房间。肝房间标记的表达式和人的 iPS 的肝相关的函数导出房间的房间被监视并且与区分的人的 ES 房间和主要人的 hepatocytes 的相比。在白天 7 点的约 60% 区分的人的 iPS 房间表示了肝的标记 alpha fetoprotein 和白长袍的。在白天 21 点的区分的房间包括白朊 Asecretion,肝糖合成,脲生产和可诱导的细胞色素 P450 活动展出了肝房间功能。肝的标记的表示和 iPS 的肝相关的功能导出房间的肝的房间比得上人的 ES 导出房间的肝的房间的。这些结果证明人的 iPS 房间,类似于人的 ES 房间,能高效地被导致区分房间进象 hepatocyte 一样。 | Zhihua Song Jun Cai Yanxia Liu Dongxin Zhao Jun Yong Shuguang Duo Xijun Song Yushan Guo Yang Zhao Han Qin Xiaolei Yin Chen Wu Jie Che Shichun Lu Mingxiao Ding Hongkui Deng | 2009 | Cell Research2009，19，11： | 63 |
| 2 | In vitro derivation of functional insulin-producing cells from human embryonic stem cells显示文摘为人的胚胎的茎(ES ) 的自强和区别的能力细胞为对待类型 Idiabetes mellitus 为胰腺的贝它细胞的产生使他们成为潜在的来源。这里,我们报导一最新发展了并且有效方法,在aserum免费的系统执行了,区分进生产胰岛素的 cells.Activin A 的导致的人的 ES 房间它在起始的阶段被使用从人的 EScells 导致权威的内胚叶区别,是由权威的内胚叶标记 Sox17 和 Brachyury.Further 的表示检测了, all-trans retinoic 酸( RA )被用来支持胰腺的区别,由早胰腺的抄写因素 pdx1 和 hlxb9 的表示显示了。在成熟 inDMEM/F12 以后有 bFGF 和菸碱的没有浆液的媒介,区分的房间表示了小岛特定的标记象 C 肽,胰岛素,胰高血糖素和 glut2 那样。百分比 ofC-peptide-positive 房间超过了 15% 。由这些房间的胰岛素和 C 肽的分泌物在葡萄糖层次对应于变化。当移植了进肾的囊时, ofStreptozotocin (STZ ) 对待裸体老鼠,这些区分的人的 ES 房间熬过并且维持贝它房间标记基因的表示包括 C 肽, pdx1, glucokinase, nkx6.1, IAPP, pax6and Tcf1。百分之三十只移植裸体老鼠展出了 stableeuglycemia 的明显的恢复;并且改正的显型被支撑超过六个星期。我们的新方法为学习人的胰开发的机制提供一个有希望的试管内区别模特儿并且说明为类型 Idiabetes mellitus 的处理使用人的 ES 房间的潜力。 | Wei Jiang Yan Shi Dongxin Zhao Song Chen Jun Yong Jing Zhang Tingting Qing Xiaoning Sun Peng Zhang Mingxiao Ding Dongsheng Li Hongkui Deng | 2007 | Cell Research2007，17，4： | 38 |
| 3 | Mechanism of electroacupuncture on inflammatory pain: neural-immune-endocrine interactions显示文摘Nociceptive signals are transmitted by peripheral afferents to the central nervous system under pain condition, a process that involves various neurotransmitters and pathways. Electroacupuncture (EA) has been widely used as a pain management technique in clinical practice. Emerging studies have shown that EA can inhibit the induction and transmission of pain signals and, consequently, mediate anti-nociceptive and anti-inflammatory effects by rebalancing the neural-immune-endocrine interactions. This review summarizes the neural-immune- endocrine circuit including peripheral afferent and central efferent, contributing to EA-induced neuroimmune and neuroendocrine modulation in inflammatory pain models. The peripheral afferent circuit includes crosstalk among immune cells, inflammatory cytokines, peripheral nociceptors. In central efferent primarily involves the neuroinflammatory interactions between spinal nociceptive neurons and glial cells. Furthermore, the hypothalamic- pituitary-adrenal axis, sympathetic and vagal nervous may serve as an essential pathway involved in the mechanism of acupuncture-mediated analgesia within the interactions of the central, immune and endocrine systems. Overall, this review focuses on the interactions of neural-immune-endocrine in inflammatory pain, which may be underlying the mechanism of EA-induced anti-inflammatory and antinociceptive effect. | Li Yuan Yang Mingxiao Wu Fan Cheng Ke Chen Haiyong Shen Xueyong Lao Lixing | 2019 | Journal of Traditional Chinese Medicine2019，39，5： | 20 |
| 4 | TGFβ inhibition enhances the generation of hematopoietic progenitors from human ES cell-derived hemogenic endothelial cells using a stepwise strategy显示文摘 | Chengyan Wang Xuming Tang Xiaomeng Sun Zhenchuan Miao Yaxin Lv Yanlei Yang Huidan Zhang Pengbo Zhang Yang Liu LiyingDu Yang Gao Ming Yin Mingxiao Ding Hongkui Deng | 2012 | Cell Research2012，22，1： | 17 |
| 5 | Effects of Citral on Aspergillus flavus Spores by Quasi-elastic Light Scattering and Multiplex Microanalysis Techniques显示文摘Citral refined from Litsea cubeba oil has been found to have a strong influence on fungi, especially Aspergillus flavus. Multiplex microanalysis and quasi-elastic light scattering techniques were applied to study the effects of citral on Aspergillus flavus spores from the levels of membrane, organelle and intracellular macromolecule. It was found that citral injured the wall and the membrane of A. flavus spore, resulting in decrease of its elasticity. After entering the cell, citral not only influenced the genetic expression of mitochondrion reduplication and its morphology, but also changed the aggregation of protein-like macromolecules. As a result, cells, organelles and macromolecules lost their normal structures and functions, eventually leading to the loss of germination ability of A. flavus spores. Since Litsea cubeba oil as food additive and antifungal agent is safe and less poisonous, it is important to elucidate the inhibitory mechanisms of Litsea cubeba oil on the germination ability of A. flavus spore. | ManLUO Li-KeJIANG Yao-XiongHUANG MingXIAO BoLI Guo-LinZOU | 2004 | Acta Biochimica et Biophysica Sinica2004，36，4： | 12 |
| 6 | Prognostic implications of epidermal growth factor receptor variant Ⅲ expression and nuclear translocation in Chinese human gliomas显示文摘Objective: To determine the prognostic implications and clinical significance of epidermal growth factor receptor variant Ⅲ(EGFRvⅢ) expression and EGFRvⅢ nuclear translocation in Chinese human gliomas.Methods: We retrospectively examined EGFRvⅢ expression and EGFRvⅢ nuclear translocation using immunohistochemistry in specimens of 240 Chinese patients with glioma, including 84 World Health Organization(WHO) II gliomas, 84 WHO Ⅲ gliomas and 72 glioblastomas(WHO IV). Factors that correlated with EGFRvⅢ and EGFRvⅢ nuclear translocation expression were analyzed by the Chi-square test. Kaplan-Meier methodology and Cox regression were used for the survival analysis.Results: Log-rank tests showed that patient age, Karnofsky performance scale(KPS) score, tumor grade,EGFRvⅢ expression, EGFRvⅢ nuclear translocation, 1 p/19 q codeletion, isocitrate dehydrogenase(IDH)mutation, Ki-67 labeling index and O6-methylguanine-DNA methyltransferase(MGMT) status(P<0.05) were significantly correlated with overall survival(OS) time. Multivariate Cox regression analysis revealed that patient age, tumor grade, EGFRvⅢ nuclear translocation, 1 p/19 q codeletion, and IDH mutation(P<0.05) were significantly correlated with OS. Patients with a high level of EGFRvⅢ nuclear translocation(≥7%) had both significantly shorter OS [hazard ratio(HR): 1.920, 95% confidence interval(95% CI): 1.228-3.003, P=0.004] and progression-free survival(PFS) times(HR: 1.661, 95% CI: 1.116-2.471, P=0.012) than those with a low level of EGFRvⅢ nuclear translocation(<7%).Conclusions: A high level of EGFRvⅢ nuclear translocation in glioma is an independent factor indicating a poor prognosis, but EGFRvⅢ expression is not an independent clinical prognostic factor. The level of EGFRvⅢ nuclear translocation maybe a novel and crucial prognostic biomarker in glioma. | Kaiyuan Yang Xiaohui Ren Liyuan Tao Peipei Wang Haihui Jiang Li Shen Yiming Zhao Yong Cui Mingxiao Li Song Lin | 2019 | Chinese Journal of Cancer Research2019，31，1： | 8 |
| 7 | Effect of Ni addition on the glass-forming ability and soft-magnetic properties of FeNiBPNb metallic glasses显示文摘The effect of Ni addition on the glass-forming ability (GFA) and soft-magnetic properties of an (Fe1-xNix)75.5B14.5P7Nb3 (x=0-0.6) alloy system were investigated. We found that the addition of Ni was effective in allowing the alloy to approach a eutectic point as well as increasing the thermal stability of the supercooled liquid. By increasing the amount of Ni,the supercooled liquid region (ΔTx),the reduced glass transition temperature Trg (Tg/Tl) and the Y parameter [Tx/(Tg+Tl)] increased from 49 to 75 K,0.540 to 0.594 and 0.373 to 0.405,respectively. As a result,novel (Fe1-xNix)75.5B14.5P7Nb3 bulk metallic glass (BMG) rods with a maximum diameter of 1 mm were synthesized with a composition of x=0.5 and x=0.6 by copper mold casting. These metallic glasses have a moderate saturation magnetization of 0.37-1.20 T with good soft-magnetic properties,i.e. a low coercivity of 1.4-3.3 A/m and a high effective permeability of 13100-20900 at 1 kHz. The simultaneous achievement of a high GFA and good soft-magnetic properties for the FeNi-based alloy system is promising for the future development of BMGs. | WANG AnDing ZHANG MingXiao ZHANG JianHua MEN He SHEN BaoLong PANG ShuJie ZHANG Tao | 2011 | Chinese Science Bulletin2011，56，36： | 7 |
| 8 | Relationship between encephalopathy and portal vein-vena cava shunt:Value of computed tomography during arterial portography显示文摘AIM: TO assess the value of computed tomography during arterial portography (CTAP) in portal vein-vena cava shunt,and analysis of the episode risk in encephalopathy.METHODS: Twenty-nine patients with portal-systemic encephalopathy due to portal hypertension were classified by West Haven method into grade Ⅰ(29 cases), gradeⅡ(16 cases), grade Ⅲ(10 cases), grade Ⅳ( 4 cases). All the patients were scanned by spiraI-CT. Plane scans, artery phase and portal vein phase enhancement scans were performed, and the source images were thinly reconstructed to 1.25 mm. We reconstructed the celiac trunk, portal vein,inferior vena cava and their branches and subjected them to three-dimensional vessel analysis by volume rendering(VR) technique and multiplanar volume reconstruction (MPVR) technique. The blood vessel reconstruction technique was used to evaluate the scope and extent of portal vein-vena cava shunt, portal vein emboli and the fistula of hepatic artery- portal vein. The relationship between the episode risk of portal-systemic encephalopathy and the scope and extent of portal vein-vena cava shunt,portal vein emboli and fistula of hepatic artery- portal vein was studied.RESULTS: The three-dimensional vessel reconstruction technique of spiraI-CT could display celiac trunk, portal vein,inferior vena cava and their branches at any planes and angles and the scope and extent of portal vein-vena cavashunt, portal vein emboli and the fistula of hepatic artery- portal vein. In twenty-nine patients with portal-systemicencephalopathy, grade Ⅰ accounted for 89.7% esophageal varices, 86.2% paragastric varices; grade Ⅱ accounted for 68.75% cirsomphalos, 56.25% paraesophageal varices,62.5% retroperitoneal varices and 81.25% dilated azygos vein; grade Ⅲ accounted for 80% cirsomphalos, 60%paraesophageal varices, 70% retroperitoneal varices, 90% dilated azygos vein, and part of the patients in grades Ⅱand Ⅲ had portal vein emboli and fistula of hepatic arteryportal vein; grade Ⅳ accounted for 75% dilated left renal vein, 50% paragallbladder varices, all the patients had fistula of hepatic artery- portal vein.CONCLUSION: The three-dimensional vessel reconstruction technique of spiraI-CT can clearly display celiac trunk, portal vein, inferior vena cava and their branches at any planes and angles and the scope and extent of portal vein-vena cava shunt. The technique is valuable for evaluating the episode risk in portal-systemic encephalopathy. | QianChu ZhenLi Su-MingZhang Dao-YuHu MingXiao | 2004 | World Journal of Gastroenterology2004，10，13： | 6 |
| 9 | New insights of Sacbrood virus显示文摘Over the last several years,major efforts have been expended to study viral infection of honeybees mainly due to colony losses around the world(Allen M,et al.,1996).It seems that honeybees are infected with numerous viruses mounting to 18so far.Infection may be asymptomatic but could still have adverse effects on the bee and may even cause death resulting in colony collapse.Sacbrood virus(SBV)is the most widely distributed of all honey bee viruses. | Mingxiao Ma | 2014 | Virologica Sinica2014，29，6： | 5 |
| 10 | Pancreas-specific Pten deficiency causes partial resistance to diabetes and elevated hepatic AKT signaling显示文摘PTEN, phosphatidylinositol-3-kinase/AKT 小径的一个否定管理者,是胰岛素发信号的一个重要调节的人。决定胰腺的 Pten 的新陈代谢的函数,我们产生了胰特定的 Pten 大美人(PPKO ) 鼠标。PPKO 老鼠扩大了胰并且提高了 acinar 房间的增长。他们也展出了低血糖症, hypoinsulinemia,和改变的氨基的新陈代谢。尤其是, PPKO 老鼠证明 streptozotocin (STZ ) 的推迟的发作导致了糖尿病,到 high-fat-diet (HFD ) 的偏导性的抵抗导致了糖尿病。在 PPKO 老鼠为抵抗调查机制到导致 HFD 的多糖症,我们在主要胰岛素应答的纸巾评估了 AKT phosphorylation:肝,肌肉,和脂肪。我们发现在胰的 Pten 损失引起在肝发信号的 AKT 的举起。AKT 和它的下游的底层 GSK3 尾的 phosphorylation 在 PPKO 老鼠的肝被增加,当没有 Pten 等位基因的可检测的切除, PTEN 水平在 PPKO 老鼠的肝被减少时。戏剧性地揭示的 Proteomics 分析减少了在 PPKO 老鼠的肝 78-kDa 铺平调整葡萄糖的蛋白质(GRP78 ) ,它可以也贡献用 HFD 喂的 PPKO 老鼠的更低的血葡萄糖水平。一起,我们的调查结果在新陈代谢的规定在肝揭示新奇回答到胰腺的缺点,把一种新尺寸加到理解糖尿病抵抗。 | Zan Tong Yan Fan Weiqi Zhang Jun Xu Jing Cheng Mingxiao Ding Hongkui Deng | 2009 | Cell Research2009，19，6： | 5 |
| 11 | Tubeimoside-1 induces TFEB-dependent lysosomal degradation of PD-L1 and promotes antitumor immunity by targeting mTOR显示文摘Programmed cell death ligand 1(PD-L1)/programmed cell death protein 1(PD-1)cascade is an effective therapeutic target for immune checkpoint blockade(ICB)therapy.Targeting PD-L1/PD-1 axis by small-molecule drug is an attractive approach to enhance antitumor immunity.Using flow cytometry-based assay,we identify tubeimoside-1(TBM-1)as a promising antitumor immune modulator that negatively regulates PD-L1 level.TBM-1 disrupts PD-1/PD-L1 interaction and enhances the cytotoxicity of T cells toward cancer cells through decreasing the abundance of PD-L1.Furthermore,TBM-1 exerts its antitumor effect in mice bearing Lewis lung carcinoma(LLC)and B16 melanoma tumor xenograft via activating tumor-infiltrating T-cell immunity.Mechanistically,TBM-1 triggers PD-L1 lysosomal degradation in a TFEB-dependent,autophagy-independent pathway.TBM-1 selectively binds to the mammalian target of rapamycin(m TOR)kinase and suppresses the activation of m TORC1,leading to the nuclear translocation of TFEB and lysosome biogenesis.Moreover,the combination of TBM-1 and anti-CTLA-4 effectively enhances antitumor T-cell immunity and reduces immunosuppressive infiltration of myeloid-derived suppressor cells(MDSCs)and regulatory T(Treg)cells.Our findings reveal a previously unrecognized antitumor mechanism of TBM-1 and represent an alternative ICB therapeutic strategy to enhance the efficacy of cancer immunotherapy. | Xiaojia Liu Mingxiao Yin Jingwen Dong Genxiang Mao Wenjian Min Zean Kuang Peng Yang Lu Liu Na Zhang Hongbin Deng | 2021 | Acta Pharmaceutica Sinica B2021，11，10： | 5 |
| 12 | Review of interface tailoring techniques and applications to improve insulation performance显示文摘Interfaces between different materials,for instance,electrode-dielectrics and vacuum/air/SF6‐insulators and oilpaper,are universal in high‐voltage insulation systems.Targeted tailoring of the interfacial properties,e.g.chemical structures,micro‐/nanoscale morphology,the charge injection barrier,trap states,and surface conductivity,is thought to be an effective approach to improve insulation properties such as breakdown and flashover strength,corona/tracking resistance,and wettability.In this article,the authors review recent progress in interface tailoring methods and their application to improve various insulation properties.The potential application scenarios of interface tailoring in different facilities are first summarised,and the desired insulation properties of various applications are highlighted.Interface tailoring methods are classified as physical/chemical surface modification and surface coating(inorganic,organic and composite),and the features of different techniques are described in detail.The structure-property relationships between interfacial states and various microscopic parameters such as charge injection barrier,trap distribution and surface conductivity are discussed together with the tailoring mechanisms for different insulation properties.Finally,the future development prospects of interface tailoring methods and their applications,e.g.multi-functional coating and stimuli-response smart coating,are presented. | Mingxiao Zhu Jianyi Xue Yanhui Wei Guochang Li Guanjun Zhang | 2022 | High Voltage2022，7，1： | 4 |
| 13 | Analysis for remedial alternatives of unregulated municipal solid waste landfills leachate-contaminated groundwater显示文摘地下水流动和溶质运输模型为预报沾染物运输并且基于 unregulated 垃圾堆的案例研究估计补救选择的效果用视觉 Modflow 被开发在东方中国的污染 leachate 的地下水。结果证明那根砷羽毛是到达在八年,和砷羽毛的最长侧面、纵的距离以后在下游的农田抽很好是分别地到达 200 m 和 260 m。但是砷羽毛的区域是不显然从八年增加到十年和羽毛的高集中的区域是在 20 年以后传播到下游的河和农田区域;当垃圾堆磨擦了时被变硬,羽毛是不在八年以后到达下游的农田区域;当抽的井下游地在羽毛被安装,分泌物率是 200m ^ 时,啜 3 ^/d,羽毛将有效地被制止;为防漏的障碍,它可能有效地在一个程度时间范围以内保护敏感目标的地下水。要不是连续的点来源,羽毛仍然是防漏的障碍到圆;当排水阴沟的分泌物率是 170.26 m ^ 时,啜 3 ^/d,羽毛有效地被控制;有排水阴沟的联合地面变硬能在控制沾染物散开给最好的效果的全面方法,和分泌物率将被归结为 111.43 m ^ 啜 3 ^/d。因此,当 leachate 漏发生在 unregulated 垃圾堆时,有排水阴沟的全面补救其他的联合地面变硬将为阻止地下水污染被推荐[出版摘要] | Da AN Yonghai JIANG Beidou XI Zhifei MA Yu YANG Queping YANG Mingxiao LI Jinbao ZHANG Shunguo BAI Lei JIANG | 2013 | Frontiers of Earth Science2013，7，3： | 3 |
| 14 | Relation between the characteristics of strong earthquake activities in Chinese mainland and the Wenchuan earthquake显示文摘This paper studies the relations between the great Wenchuan earthquake and the active-quiet periodic characteristics of strong earthquakes,the rhythmic feature of great earthquakes,and the grouped spatial distribution of MS8.0 earthquakes in Chinese mainland.We also studied the relation between the Wenchuan earthquake and the stepwise migration characteristics of MS≥7.0 earthquakes on the North-South seismic belt,the features of the energy releasing acceleration in the active crustal blocks related to the Wenchuan earthquake and the relation between the Wenchuan earthquake and the so called secondarc fault zone.The results can be summarized as follows:1 the occurrence of the Wenchuan earthquake was consistent with the active-quiet periodic characteristics of strong earthquakes;2 its occurrence is consistent with the features of grouped occurrence of MS8.0 earthquakes and follows the 25 years rhythm(each circulation experiences the same time) of great earthquakes;3 the Wenchuan MS8.0 earthquake follows the well known stepwise migration feature of strong earthquakes on the North-South seismic belt;4 the location where the Wenchuan MS8.0 earthquake took place has an obvious consistency with the temporal and spatial characteristic of grouped activity of MS≥7.0 strong earthquakes on the secondarc fault zone;5 the second-arc fault zone is not only the lower boundary for earthquakes with more than 30 km focal depth,but also looks like a lower boundary for deep substance movement;and 6 there are obvious seismic accelerations nearby the Qaidam and Qiangtang active crustal blocks(the northern and southern neighbors of the Bayan Har active block,respectively),which agrees with the GPS observation data. | Xiaodong Zhang Guohua Yang Xian Lu Mingxiao Li Zhigao Yang | 2009 | Earthquake Science2009，22，5： | 3 |
| 15 | Corrosion Behavior of 316L Stainless Steels Exposed to Salt Lake Atmosphere of Western China for 8 years显示文摘The evolution of corrosion behavior of 316 L stainless steels exposed to salt lake atmosphere for 8 years was investigated.The results showed that the stainless steel in salt lake atmosphere had a greater corrosion rate during the initial exposure time and relatively lower corrosion rate during the subsequent exposure time.Dust depositions accumulated on the downward surface caused severe local corrosion of stainless steel.As exposure time prolonged,the relative amount of Croxide and Feoxidein the corrosion products gradually increased,which may directly affect the corrosion rate of stainless steels.Moreover,the maximum pit depth followed a power function with respect to exposure time. | Mingxiao Guo Junrong Tang Tianzhen Gu Can Peng Qiaoxia Li Chen Pan Zhenyao Wang | 2021 | Acta Metallurgica Sinica(English Letters)2021，34，4： | 3 |
| 16 | Gold Nanoparticles for Cancer Theranostics显示文摘金 nanoparticles 在生物医学的场地里看见了崭新的开发,特别地为癌症治疗。因为他们的容易的准备, functionalization, biocompatibility, non-cytotoxicity,和 detectability,他们收到了广泛的注意。Functionalized 金 nanoparticles 能在药和基因的地里被使用交货, photothermal 治疗,和 bioimaging。这评论为准备金 nanoparticles 的各种各样的形状介绍方法并且在癌症治疗的场地里描述他们的当前的应用。而且,评论在临床的 theranostics 介绍发展线路和金 nanoparticles 的当前的问题。 | Hong Liang Huayu Tian Mingxiao Deng Xuesi Chen | 2015 | Chinese Journal of Chemistry2015，33，9： | 3 |
| 17 | Seismic comprehensive forecast based on modified project pursuit regression显示文摘In the research of projection pursuit for seismic comprehensive forecast,the algorithm of projection pursuit regression(PPR) is one of most applicable methods.But generally,the algorithm structure of the PPR is very complicated.By partial smooth regressions for many times,it has a large amount of calculation and complicated extrapolation,so it is easily trapped in partial solution.On the basis of the algorithm features of the PPR method,some solutions are given as below to aim at some shortcomings in the PPR calculation:to optimize project direction by using particle swarm optimization instead of Gauss-Newton algorithm,to simplify the optimal process with fitting ridge function by using Hermitian polynomial instead of piecewise linear regression.The overall optimal ridge function can be obtained without grouping the parameter optimization.The modeling capability and calculating accuracy of projection pursuit method are tested by means of numerical emulation technique on the basis of particle swarm optimization and Hermitian polynomial,and then applied to the seismic comprehensive forecasting models of polydimensional seismic time series and general disorder seismic samples.The calculation and analysis show that the projection pursuit model in this paper is characterized by simplicity,celerity and effectiveness.And this model is approved to have satisfactory effects in the real seismic comprehensive forecasting,which can be regarded as a comprehensive analysis method in seismic comprehensive forecast. | Anxu Wu Xiangdong Lin Changsheng Jiang Yongxian Zhang Xiaodong Zhang Mingxiao Li Ping'an Li | 2009 | Earthquake Science2009，22，5： | 3 |
| 18 | Advances in Fe(Ⅲ)bioreduction and its application prospect for groundwater remediation:A review显示文摘Microbial Fe(Ⅲ)reduction is a significant driving force for the biogeochemical cycles of C,O,P,S,N,and dominates the natural bio-purification of contaminants in groundwater(e.g.,petroleum hydrocarbons,chlorinated ethane,and chromium).In this review,the mechanisms and environmental significance of Fe(Ⅲ)(hydro)oxides bioreduction are summarized.Compared with crystalline Fe(Ⅲ)(hydro)oxides,amorphous Fe(m)(hydro)oxides are more bioavailable.Ligand and electron shuttle both play an important role in microbial Fe(Ⅲ)reduction.The restrictive factors of Fe(Ⅲ)(hydro)oxides bioreduction should be further investigated to reveal the characteristics and mechanisms of the process.It will improve the bioavailability of crystalline Fe(Ⅲ)(hydro)oxides and accelerate the anaerobic oxidation efficiency of the reduction state pollutants.Furthermore,the approach to extract,culture,and incubate the functional Fe(Ⅲ)reducing bacteria from actual complicated environment,and applying it to the bioremediation of organic,ammonia,and heavy metals contaminated groundwater will become a research topic in the future.There are a broad application prospects of Fe(Ⅲ)(hydro)oxides bioreduction to groundwater bioremediation,which includes the in situ injection and permeable reactive barriers and the innovative Kariz wells system.The study provides an important reference for the treatment of reduced pollutants in contaminated groundwater. | Yu Jiang Beidou Xi Rui Li Mingxiao Li Zheng Xu Yuning Yang Shaobo Gao | 2019 | Frontiers of Environmental Science & Engineering2019，13，6： | 3 |
| 19 | Sequencing and rescuing a highly virulent classical swine fever virus: Chinese strain cF114 from a full-length cDNA clone显示文摘The complete nucleotide sequence of classical swine fever virus (CSFV) strain cF114 (F114 strain propa- gated on PK-15 cells) was cloned by RT-PCR. The analyses of nucleotide and amino acids identity between cF114 and F114, Brescia, Alfort or C strain were 99.41%, 96.80%, 86.03%, 95.70% and 99.28%, 98.54%, 93.33%, 97.41% re- spectively. The cDNA fragments with correct sequence were ligated into a full-length cDNA and inserted into pMC18 plasmid (pMC12297). A full-length infectious viral RNA was synthesized by runoff transcription and transfected to PK15 cells. Viruses were recovered from transfected cells which wese titrated on PK-15 cells by endpoint dilution and indirect immunofluorescence with a CSFV-specific monoclonal antibody. The antigenicity and replication kinetics of the plasmid-derived virus (vM12297) were similar to the parental virus in vitro. The E01 or E2 gene was replaced with the genes from strain C and the pM/CE01 and pM/CE2 with chimeric full-length cDNA of cF114 were generated. The infectious viruses were obtained from pM/CE01 and pM/CE2. Both of the chimeric viruses can infect PK-15, SK- 6 and primary testicle cell of swine. The chimeric viruses can grow to a titer of 8?05 F-PFU/mL. These results are very important for understanding the genes related to the CSFV propagation and pathogenesis. | NIE Yuchun CHEN Jianguo DING Mingxiao | 2003 | Chinese Science Bulletin2003，48，11： | 3 |
| 20 | Building a healthy China by enhancing physical activity:Priorities,challenges,and strategies显示文摘Dear editor,In 2016,several landmark government initiatives were launched in an effort to build a more healthy China.In March,the National People’s Congress of China adopted the 13th FiveYear Plan,which sets out new objectives,principles,and targets for 2016–2020,1including action plans for enhancing environmental protection and green growth,promoting individual | Sa Wu Yufeng Luo Xue Qiu Mingxiao Bao | 2017 | Journal of Sport and Health Science2017，6，1： | 3 |
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