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| 1 | Efficient generation of hepatocyte-like cells from human induced pluripotent stem cells显示文摘人的导致的 pluripotent 茎(iPS ) 房间类似于胚胎的茎(ES ) 房间,和罐头强烈地增殖并且区分进许多房间类型。然而,人的 iPS 房间的肝的区别还没被报导了。在这份报告,人的 iPS 房间被导致由一个逐步的协议区分进肝的房间。肝房间标记的表达式和人的 iPS 的肝相关的函数导出房间的房间被监视并且与区分的人的 ES 房间和主要人的 hepatocytes 的相比。在白天 7 点的约 60% 区分的人的 iPS 房间表示了肝的标记 alpha fetoprotein 和白长袍的。在白天 21 点的区分的房间包括白朊 Asecretion,肝糖合成,脲生产和可诱导的细胞色素 P450 活动展出了肝房间功能。肝的标记的表示和 iPS 的肝相关的功能导出房间的肝的房间比得上人的 ES 导出房间的肝的房间的。这些结果证明人的 iPS 房间,类似于人的 ES 房间,能高效地被导致区分房间进象 hepatocyte 一样。 | Zhihua Song Jun Cai Yanxia Liu Dongxin Zhao Jun Yong Shuguang Duo Xijun Song Yushan Guo Yang Zhao Han Qin Xiaolei Yin Chen Wu Jie Che Shichun Lu Mingxiao Ding Hongkui Deng | 2009 | Cell Research2009，19，11： | 63 |
| 2 | In vitro derivation of functional insulin-producing cells from human embryonic stem cells显示文摘为人的胚胎的茎(ES ) 的自强和区别的能力细胞为对待类型 Idiabetes mellitus 为胰腺的贝它细胞的产生使他们成为潜在的来源。这里,我们报导一最新发展了并且有效方法,在aserum免费的系统执行了,区分进生产胰岛素的 cells.Activin A 的导致的人的 ES 房间它在起始的阶段被使用从人的 EScells 导致权威的内胚叶区别,是由权威的内胚叶标记 Sox17 和 Brachyury.Further 的表示检测了, all-trans retinoic 酸( RA )被用来支持胰腺的区别,由早胰腺的抄写因素 pdx1 和 hlxb9 的表示显示了。在成熟 inDMEM/F12 以后有 bFGF 和菸碱的没有浆液的媒介,区分的房间表示了小岛特定的标记象 C 肽,胰岛素,胰高血糖素和 glut2 那样。百分比 ofC-peptide-positive 房间超过了 15% 。由这些房间的胰岛素和 C 肽的分泌物在葡萄糖层次对应于变化。当移植了进肾的囊时, ofStreptozotocin (STZ ) 对待裸体老鼠,这些区分的人的 ES 房间熬过并且维持贝它房间标记基因的表示包括 C 肽, pdx1, glucokinase, nkx6.1, IAPP, pax6and Tcf1。百分之三十只移植裸体老鼠展出了 stableeuglycemia 的明显的恢复;并且改正的显型被支撑超过六个星期。我们的新方法为学习人的胰开发的机制提供一个有希望的试管内区别模特儿并且说明为类型 Idiabetes mellitus 的处理使用人的 ES 房间的潜力。 | Wei Jiang Yan Shi Dongxin Zhao Song Chen Jun Yong Jing Zhang Tingting Qing Xiaoning Sun Peng Zhang Mingxiao Ding Dongsheng Li Hongkui Deng | 2007 | Cell Research2007，17，4： | 38 |
| 3 | TGFβ inhibition enhances the generation of hematopoietic progenitors from human ES cell-derived hemogenic endothelial cells using a stepwise strategy显示文摘 | Chengyan Wang Xuming Tang Xiaomeng Sun Zhenchuan Miao Yaxin Lv Yanlei Yang Huidan Zhang Pengbo Zhang Yang Liu LiyingDu Yang Gao Ming Yin Mingxiao Ding Hongkui Deng | 2012 | Cell Research2012，22，1： | 17 |
| 4 | Pancreas-specific Pten deficiency causes partial resistance to diabetes and elevated hepatic AKT signaling显示文摘PTEN, phosphatidylinositol-3-kinase/AKT 小径的一个否定管理者,是胰岛素发信号的一个重要调节的人。决定胰腺的 Pten 的新陈代谢的函数,我们产生了胰特定的 Pten 大美人(PPKO ) 鼠标。PPKO 老鼠扩大了胰并且提高了 acinar 房间的增长。他们也展出了低血糖症, hypoinsulinemia,和改变的氨基的新陈代谢。尤其是, PPKO 老鼠证明 streptozotocin (STZ ) 的推迟的发作导致了糖尿病,到 high-fat-diet (HFD ) 的偏导性的抵抗导致了糖尿病。在 PPKO 老鼠为抵抗调查机制到导致 HFD 的多糖症,我们在主要胰岛素应答的纸巾评估了 AKT phosphorylation:肝,肌肉,和脂肪。我们发现在胰的 Pten 损失引起在肝发信号的 AKT 的举起。AKT 和它的下游的底层 GSK3 尾的 phosphorylation 在 PPKO 老鼠的肝被增加,当没有 Pten 等位基因的可检测的切除, PTEN 水平在 PPKO 老鼠的肝被减少时。戏剧性地揭示的 Proteomics 分析减少了在 PPKO 老鼠的肝 78-kDa 铺平调整葡萄糖的蛋白质(GRP78 ) ,它可以也贡献用 HFD 喂的 PPKO 老鼠的更低的血葡萄糖水平。一起,我们的调查结果在新陈代谢的规定在肝揭示新奇回答到胰腺的缺点,把一种新尺寸加到理解糖尿病抵抗。 | Zan Tong Yan Fan Weiqi Zhang Jun Xu Jing Cheng Mingxiao Ding Hongkui Deng | 2009 | Cell Research2009，19，6： | 5 |
| 5 | Sequencing and rescuing a highly virulent classical swine fever virus: Chinese strain cF114 from a full-length cDNA clone显示文摘The complete nucleotide sequence of classical swine fever virus (CSFV) strain cF114 (F114 strain propa- gated on PK-15 cells) was cloned by RT-PCR. The analyses of nucleotide and amino acids identity between cF114 and F114, Brescia, Alfort or C strain were 99.41%, 96.80%, 86.03%, 95.70% and 99.28%, 98.54%, 93.33%, 97.41% re- spectively. The cDNA fragments with correct sequence were ligated into a full-length cDNA and inserted into pMC18 plasmid (pMC12297). A full-length infectious viral RNA was synthesized by runoff transcription and transfected to PK15 cells. Viruses were recovered from transfected cells which wese titrated on PK-15 cells by endpoint dilution and indirect immunofluorescence with a CSFV-specific monoclonal antibody. The antigenicity and replication kinetics of the plasmid-derived virus (vM12297) were similar to the parental virus in vitro. The E01 or E2 gene was replaced with the genes from strain C and the pM/CE01 and pM/CE2 with chimeric full-length cDNA of cF114 were generated. The infectious viruses were obtained from pM/CE01 and pM/CE2. Both of the chimeric viruses can infect PK-15, SK- 6 and primary testicle cell of swine. The chimeric viruses can grow to a titer of 8?05 F-PFU/mL. These results are very important for understanding the genes related to the CSFV propagation and pathogenesis. | NIE Yuchun CHEN Jianguo DING Mingxiao | 2003 | Chinese Science Bulletin2003，48，11： | 3 |
| 6 | Generation of Induced Pluripotent Stem Cells from Adult Rhesus Monkey Fibroblasts显示文摘 | Haisong Liu Fangfang Zhu Jun Yong Pengbo Zhang Pingping Hou Honggang Li Wei Jiang Jun Cai Meng Liu Kai Cui Xiuxia Qu Tingting Xiang Danyu Lu Xiaochun Chi Ge Gao Weizhi Ji Mingxiao Ding Hongkui Deng | 2008 | Cell Stem Cell2008，，6： | 1 |
| 7 | Generation of Induced Pluripotent Stem Cells from Adult Rhesus Monkey Fibroblasts显示文摘 | Haisong Liu Fangfang Zhu Jun Yong Pengbo Zhang Pingping Hou Honggang Li Wei Jiang Jun Cai Meng Liu Kai Cui Xiuxia Qu Tingting Xiang Danyu Lu Xiaochun Chi Ge Gao Weizhi Ji Mingxiao Ding Hongkui Deng | 2008 | Cell Stem Cell2008，，6： | 1 |
| 8 | Fibrillarin redistributes to the spindle poles and partially colocalizes with NuMA during mitosis显示文摘Fibrillann, a major protein in the nucleolus. is known to redistribute during mitosis from the nucleolus to the cytosol, and is related to the dynamics of post-mitotic reassembly of the nucleolus. To better understand the dynamic behavior and the relationship with other cytoplasmic structures, we have now expressed fibrillarin-pDsRedl fusion protein in HeLa cells. The results showed that a part of fibrillarin was associated with mitotic spindle poles in the mitotic cells. Nocodazole-induced microtubule depolymeri-zation resulted in fibrillarin redistribution throughout the cytoplasm, and removal of nocodazole resulted in relocaliza-tion of fibrillarin at the polar region during the mitotic spindles reassembly. In a mitotic cell free system, fibrillarin was found in the center of taxol-induced microtubule asters. Moreover, fibrillarin was found to colocalize with the nuclear mitotic apparatus protein (NuMA) at the poles of mitotic cells. Therefore, it is postulated that the polar redistribution of | Yan Guo Chaoliang Wei Jinsong Hu Mingxiao Ding Jianguo Chen | 2002 | Chinese Science Bulletin2002，47，23： | 0 |
| 9 | N-terminal of Lprotein of vesicular stomatitis virus contains a new signal sequence显示文摘The L protein (241 kD) of vesicular stomatitis virus (VSV) is the most important subunit of the replication complex. The existence of specific localization signal in the L protein was investigated by making recombinant constructs expressing truncated mutants of the L protein fused to green fluorescent protein (GFP) in transient transfection assays. The chimeric genes encoding varied N-terminal of L and GFP gene were put under the control of T7 promoter or CMV promoter. The fusion proteins were transiently expressed in BHK-21, COS-7, CHO or Hep G2 cells. When more than 120 residues were deleted or only 96 residues were kept on the N-terminal, the fusion proteins were shown to be distributed throughout the cells, cytoplasm and nucleus under the confocal microscope. However, other chimeric proteins with 120 or more amino acids were dotted and distributed in the perinuclear regions. And the fusion protein with 96120 aa has the similar distribution. A thirteen-residue peptide QGYSFLHEVDKEA (108120) was identified as localization signal, whose function would be absolutely distributed with the deficiency of D or V. Our results show that there is an independent localizing signal in N-terminal domain of L protein of VSV and this functional signal is conserved in different cell lines. | NIE Yuchun, KE Yeyan, WANG Zai, YU Xiang, DENG Hongkui & DING Mingxiao Department of Cell Biology and Genetics, College of Life Sciences, Peking University, Beijing 100871, China Correspondence should be addressed to Ding Mingxiao (e-mail: mxding@china.com.cn) | 2003 | Chinese Science Bulletin2003，48，13： | 0 |
| 10 | Nanog reporter system in mouse embryonic stem cells based on highly efficient BAC homologous recombination显示文摘Nanog is a novel transcription factor specifically expressed in mouse embryonic stem cells (mES cells). It has been reported that Nanog plays an essential role in maintaining multi-potency of ES cells. The expression of Nanog is very sensitive to ES cells differentiation, making Nanog one of the best markers to indicate the status of ES cells. In this study, we developed an efficient method to construct Nanog promoter driven EGFP reporter system based on the BAC homologous recombination. We further generated a Nanog-EGFP reporter mES cell line. This reporter mES cell line exhibited features similar to those of normal mES cells, and the EGFP reporter efficiently reflected the expression of Nanog, indi- cating the differentiation status of mES cells. We achieved a reliable experimental reporter system to research self-renewal and differentiation of mES cells. The system could facilitate research on culture system of mES cells and researches on the expression and regulation of Nanog and other related fac- tors in mES cells. | FAN Yan TONG Zan YOU JieFang DU LiYing ZOU FangDong DING MingXiao DENG HongKui | 2007 | Chinese Science Bulletin2007，52，20： | 0 |
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