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| 1 | Tobacco curly shoot virus isolated in Yunnan is a distinct species of Begomovirus显示文摘Virus isolate Y1 was obtained from tobacco showing curly shoot symptoms in Baoshan, Yunnan Province. Whitefly transmission test and virion morphology observa-tion showed that it is a begomovirus. In reactions with 14 monoclonal antibodies raised against begomoviruses, Y1 was readily differentiated from begomoviruses reported in China, Pakistan and India. The complete nucleotide sequence of DNA-A was determined, it contains 2746 nucleotides, with two ORFs in virion-sense DNA and four ORFs in comple-mentary-sense DNA. Comparisons with total DNA-A, inter-genie region and deduced amino acid sequences of individual ORFs showed that Y1 is a distinct Begomovirus species, for which the name Tobacco curly shoot virus (TCSV) is pro-posed. The total DNA-A of TCSV is most closely related to that of Tomato leaf curl virus from India (85% sequence identity). In contrast, the deduced coat protein of TCSV is most like that of Cotton leaf curl virus 72b isolate from Paki-stan (98% amino acid sequence identity). | XIE Yan ZHOU Xueping ZHANG Zhongkai QI Yijun | 2002 | Chinese Science Bulletin2002，47，3： | 51 |
| 2 | Sulfonated polyaniline/vanadate composite as anode material and its electrochemical property in microbial fuel cells on ocean floor显示文摘A unique sulfonated polyaniline/vanadate composite was synthesized and utilized as a composite anode in microbial fuel cells on ocean floor (BMFCs). X-ray diffraction (XRD) and thermogravimetric analysis (TGA) were employed to characterize its chemical composition and morphology. Wettability of the composite anodes decreases due to the addition of polytetrafluoroethylene (PTFE). The electrochemical behavior of the composite anodes was investigated by means of linear sweep voltammetry and Tafel plot measurements. Compared with the plain graphite anode,the composite anode significantly improves the power density,5.5-fold higher,reaching 187.1 mW/m2 and gives a 27-fold higher exchange current density and a higher kinetic activity. A novel synergistic mechanism between sulfonated polyaniline and vanadate is proposed to explain the excellent electrochemical performance. This composite thus has great potential to be used as an anode material for a high-power BMFC. | FU YuBin ZHAO ZhongKai LIU Jia LI KuiZhong XU Qian ZHANG ShaoYun | 2011 | Science China Chemistry2011，54，5： | 8 |
| 3 | Purification and Characterization of a New Ribosome Inactivating Protein from Cinchonaglycoside C-treated Tobacco Leaves显示文摘A new ribosome-inactivating protein(RIP)with a molecular weight of 31 kDa induced by Cinchonaglycoside C(1)designatedCLP31,was isolated from tobacco leaves.Analysis of this protein sequence indicated that it belongs to the RIP family and itwas distinct from the other plant RIPs reported previously at its N-terminal amino acid sequence.CIP31 can directly impairsynthesis of coat protein(CP)of tobacco mosaic virus(TMV),which resulted in inhibition of TMV long distance movementand multiplication in tobacco plants at concentrations of ng/mL.Furthermore,no toxicity was shown to the growth andfertility of the plants.CIP31 was synthesized only in the presence of Cinchonaglycoside C(1)and was independent of thesalicylic acid(SA)signal pathway.We provided evidence for the SA-independent biological induction of resistance. | Yanmei Li Yantao Jia Zhongkai Zhang Xiaoying Chen Hongping He Rongxiang Fang Xiaojiang Hao | 2007 | Journal of Integrative Plant Biology2007，49，9： | 8 |
| 4 | Identification of a novel DNA molecule associated with Tobacco leaf curl virus显示文摘A circular DNA molecule, designated as DNAp, was identified in tobacco plants infected with Tobacco leaf curl virus (TLCV) isolates Y5 and Y8 by PCR using primers based on the conserved region of the two reported DNAp sequences of whitefly-transmitted geminiviruses (WTGs). The complete nucleotide sequences of DNAβ of Y5 and Y8 (TLCV DNAβ) were determined. Y5 DNAβ comprises 1333 nucleotides encoding 8 predicted ORFs with 4 ORFs in virion-sense DNA and 4 ORFs in complementary-sense DNA; Y8 DNAP consists of 1338 nucleotides encoding 7 predicted ORFs with 4 ORFs in virion-sense DNA and 3 ORFs in complementary-sense DNA. TLCV DNAβhas little sequence homology to DNA-A of TLCV, except that it shares conserved TAATATTAC loop sequence with TLCV DNA-A. Sequence comparison showed that Y5 DNAβ shared 85% sequence homology with Y8 DNAβ, and both Y5 DNAβ and Y8 DNAβ had relatively low sequence identity (51%-65%) with the reported DNAβ molecules associated with Agera-tum yellow vein virus and Cotton leaf | XIE Yan ZHOU Xueping LI Zhenghe ZHANG Zhongkai LI Guixin | 2002 | Chinese Science Bulletin2002，47，15： | 6 |
| 5 | Fragility assessment of tunnels in soft soils using artificial neural networks显示文摘Recent earthquakes have shown that tunnels are prone to damage,posing a major threat to safety and having major cascading and socioeconomic impacts.Therefore,reliable models are needed for the seismic fragility assessment of underground structures and the quantitative evaluation of expected losses.Based on previous researches,this paper presented a probabilistic framework based on an artificial neural network(ANN),aiming at the development of fragility curves for circular tunnels in soft soils.Initially,a two-dimensional incremental dynamic analysis of the nonlinear soil-tunnel system was performed to estimate the response of the tunnel under ground shaking.The effects of soil-structure-interaction and the ground motion characteristics on the seismic response and the fragility of tunnels were adequately considered within the proposed framework.An ANN was employed to develop a probabilistic seismic demand model,and its results were compared with the traditional linear regression models.Fragility curves were generated for various damage states,accounting for the associated uncertainties.The results indicate that the proposed ANN-based probabilistic framework can results in reliable fragility models,having similar capabilities as the traditional approaches,and a lower computational cost is required.The proposed fragility models can be adopted for the risk analysis of typical circular tunnel in soft soils subjected to seismic loading,and they are expected to facilitate decision-making and risk management toward more resilient transport infrastructure. | Zhongkai Huang Sotirios A.Argyroudis Kyriazis Pitilakis Dongmei Zhang Grigorios Tsinidis | 2022 | Underground Space2022，7，2： | 4 |
| 6 | Distribution Features of the Nanhua-Sinian Rifts and their Significance to Hydrocarbon Accumulation in the Tarim Basin显示文摘On the basis of reprocessing 34 new two-dimensional spliced long sections(20,191 km) in the Tarim Basin, the deep structure features of the Tarim Basin were analyzed through interpreting 30,451 km of two-dimensional seismic data and compiling basic maps. Seismic interpretation and geological analysis conclude that the Nanhua-Sinian strata are a set of rift-depression depositional systems according to their tectonic and depositional features. The rift valley formed in the Nanhua Period, and the transformation became weaker during the late Sinian Period, which eventually turned into depression. From bottom to top, the deposited strata include mafic igneous, tillite, mudstone, and dolomite. Three major depocenters developed inside this basin during the rift stage and are distributed in the eastern Tarim Basin, the Awati area, and the southwestern Tarim Basin. Among them, the rift in the eastern Tarim Basin strikes in the near east-west direction on the plane and coincides with the aeromagnetic anomaly belt. This represents a strong magnetic zone formed by upwelling basic volcanic rock along high, steep normal faults of the Nanhua Period. Controlled by the tectonic background, two types of sedimentary systems were developed in the rift stage and depression stage, showing two types of sequence features in the Sinian depositional stage. The Nanhua System appears as a wedge-shaped formation, with its bottom in unconformable contact with the base. The rifting event has a strong influence on the current tectonic units in the Tarim Basin, and affects the distribution of source rock in the Yuertus Formation and reservoir beds in the Xiaoerbulake Formation in Lower Cambrian, as well as the gypseous cap rock in Middle Cambrian. The distribution features of the rifts have important and realistic significance for determining the direction of oil and gas exploration in the deep strata of the Tarim Basin. Comprehensive analysis suggests that the Tazhong region is the most favorable zone, and the Kalpin-Bachu region is the optimal potential zone for exploring sub-salt oil and gas in deep Cambrian strata. | BAI Zhongkai CHENG Minghua CHEN Cheng HUO Junzhou ZHANG Junfeng ZHOU Xingui GAO Yongjin ZHAO Bo QIU Haijun HAN Miao YANG Youxing SUN Zhichao MIAO Miaoqing ZHANG Jinhu LI Qingyao | 2020 | Acta Geologica Sinica(English Edition)2020，94，2： | 3 |
| 7 | Inflation analyses of a circular elastomeric membrane with hydrostatic pressure显示文摘Composite diaphragm inflation(CDI) experiment is a useful methodology for exploring the mechanics relation of cell-cell junctions within a living epithelial sheet.In this paper,a new methodology of CDI experiment has been put forward.The finite deformation of a circular polydimezhylsiloxane elastomeric membrane,which is used as the substrate for the culture of epithelial cells,under hydrostatic pressure is explored by the theory of non-linear continuum mechanics.The simplified governing equations with minimum amount of differential equations and boundary conditions are obtained and solved by multiple shooting method.The influences on the membrane by hydrostatic pressure have been discussed. | Zhongkai Zhang Jianbing Sang Yunjiao Zhang | 2012 | Theoretical & Applied Mechanics Letters2012，2，6： | 3 |
| 8 | FORMING PROCESS OF HOT-EXTRUDED SiCw/6061Al COMPOSITES(Ⅰ)显示文摘1INTRODUCTIONInrecentyears,moreandmorecontinuouslyordiscontinuouslyreinforcedmetalmatrixcomposites(MMCs)havebeenusedtomakest... | Zhang Wenlong, Cai Liuchun, Peng Huaxin, Wang Dezun and Yao Zhongkai School of Materials Science and Engineering, Harbin Institute of Technology, Harbin 150001, P. R. China | 1998 | 中国有色金属学会会刊:英文版1998，8，3： | 3 |
| 9 | Silicon photonic transceivers for application in data centers显示文摘Global data traffic is growing rapidly,and the demand for optoelectronic transceivers applied in data centers(DCs)is also increasing correspondingly.In this review,we first briefly introduce the development of optoelectronics transceivers in DCs,as well as the advantages of silicon photonic chips fabricated by complementary metal oxide semiconductor process.We also summarize the research on the main components in silicon photonic transceivers.In particular,quantum dot lasers have shown great potential as light sources for silicon photonic integration—whether to adopt bonding method or monolithic integration—thanks to their unique advantages over the conventional quantum-well counterparts.Some of the solutions for highspeed optical interconnection in DCs are then discussed.Among them,wavelength division multiplexing and four-level pulseamplitude modulation have been widely studied and applied.At present,the application of coherent optical communication technology has moved from the backbone network,to the metro network,and then to DCs. | Haomiao Wang Hongyu Chai Zunren Lv Zhongkai Zhang Lei Meng Xiaoguang Yang Tao Yang | 2020 | Journal of Semiconductors2020，41，10： | 1 |
| 10 | Flexible temperature sensor with high sensitivity ranging from liquid nitrogen temperature to 1200℃显示文摘Flexible temperature sensors have been extensively investigated due to their prospect of wide application in various flexible electronic products.However,most of the current flexible temperature sensors only work well in a narrow temperature range,with their application at high or low temperatures still being a big challenge.This work proposes a flexible thermocouple temperature sensor based on aerogel blanket substrate,the temperature-sensitive layer of which uses the screen-printing technology to prepare indium oxide and indium tin oxide.It has good temperature sensitivity,with the test sensitivity reaching 226.7μV℃^(−1).Most importantly,it can work in a wide temperature range,from extremely low temperatures down to liquid nitrogen temperature to high temperatures up to 1200℃,which is difficult to be achieved by other existing flexible temperature sensors.This temperature sensor has huge application potential in biomedicine,aerospace and other fields. | Zhaojun Liu Bian Tian Zhuangde Jiang Shuimin Li Jiaming Lei Zhongkai Zhang Jiangjiang Liu Peng Shi Qijing Lin | 2023 | International Journal of Extreme Manufacturing2023，5，1： | 1 |
| 11 | Functional analysis of the promoter of the dmrt1 gene in Chinese tongue sole,Cynoglossus semilaevis显示文摘The dmrt1 (doublesex and mab-3 related transcription factor 1) gene is considered to play an essential role in testis differentiation and development among metazoan species. As an economically important marine fish in China, Chinese tongue sole (Cynoglossus semilaevis) possess a ZW/ZZ sex-determining system, and the females usually grow two to four times bigger than males. Previous studies have confirmed that Z-linked dmrt1 gene is the male determining gene in C. semilaevis and TALENs-mediated knocking-out individuals showed increased growth phenotype. In order to investigate the function of dmrt1 regulatory regions and its potential application, we have cloned the promoter of C. semilaevis dmrt1. The acquired sequence consisted of a 5'-upstream sequence and a partial exon1;functional analysis showed that there were two positive regulatory regions in the promoter, as well as one negative region and one neutral region. These positive regions were then combined to obtain several newly constructed promoters. Both in vitro and in vivo analysis confirmed one of them (dmrt1-Δ3+Δ4) showed elevated promoting activity compared to the full-length promoter and could drive exogenous gene expression in tongue sole gonads. Our results will provide useful information for understanding the regulatory mechanism of dmrt1 gene during C. semilaevis sex determination, and the endogenous promoter will facilitate the transgenic research for analyzing sex- and growth-related genes. | WANG Qian CUI Zhongkai GUO Hua ZHANG Nianwei XU Wenteng YANG Yingming CHEN Songlin | 2019 | Journal of Oceanology and Limnology2019，37，4： | 1 |
| 12 | Designing contracts for a closed-loop supply chain under information asymmetry 显示文摘 | Zhang Pan Xiong Yu Xiong Zhongkai | 2014 | Operations Research Letters2014，42，2： | 1 |
| 13 | Designing contracts for a closed-loop supply chain under informa- tion asymmetry显示文摘 | Zhang Pan Xiong Yu Xiong Zhongkai | 2014 | 42(2): 150-1552014，42，2： | 1 |
| 14 | Auto machine learning-based modelling and prediction of excavationinduced tunnel displacement显示文摘The influence of a deep excavation on existing shield tunnels nearby is a vital issue in tunnelling engineering.Whereas,there lacks robust methods to predict excavation-induced tunnel displacements.In this study,an auto machine learning(AutoML)-based approach is proposed to precisely solve the issue.Seven input parameters are considered in the database covering two physical aspects,namely soil property,and spatial characteristics of the deep excavation.The 10-fold cross-validation method is employed to overcome the scarcity of data,and promote model’s robustness.Six genetic algorithm(GA)-ML models are established as well for comparison.The results indicated that the proposed AutoML model is a comprehensive model that integrates efficiency and robustness.Importance analysis reveals that the ratio of the average shear strength to the vertical effective stress E_(ur)/σ′_(v),the excavation depth H,and the excavation width B are the most influential variables for the displacements.Finally,the AutoML model is further validated by practical engineering.The prediction results are in a good agreement with monitoring data,signifying that our model can be applied in real projects. | Dongmei Zhang Yiming Shen Zhongkai Huang Xiaochuang Xie | 2022 | Journal of Rock Mechanics and Geotechnical Engineering2022，14，4： | 1 |
| 15 | TURBULENT STRUCTURE AND LOCAL SIMILARITY IN THE TOWER LAYER OVER THE NANJING AREA显示文摘 | XU YUMAO ZHOU CHAOFU LI ZHONGKAI ZHANG WEI | 1997 | Boundary - Layer Meteorology1997，，1： | 1 |
| 16 | Formaldehyde and VOC emissions at different manufacturing stages of wood - based panels 显示文摘 | He Zhongkai Zhang Yinping Wei Wen- juan | 2012 | Building and Envi- ronment2012，，47： | 1 |
| 17 | Construction and characterization of the transformation-competent artificial chromosome (TAC) libraries of Leymus multicaulis显示文摘Transformation-competent artificial chromosome system is able to clone and transfer genes efficiently in plants.In order to clone genes highly tolerant to barley yellow dwarf virus(BYDV),Aphids,drought and salt from Leymus multicaulis,the two TAC genomic libraries I and II were constructed in vector pYLTAC17 and pYLTAC747H/sacB,which contain about 165000 and 236000 recombinant clones sepa-rately.The genome coverage of the two libraries was totally estimated to be about 3―5 haploid genome equivalents,as size selection of genomic DNA fragments was approximately from 9 to 300 kb.Clones of the genomic libraries were collected as bulked pools each containing 500 clones or so,stored in twelve 96-deep-well plates and then were gridding in triplicate onto a high-density colony hybridization filter with a 3×3 pattern using a GeneTAC?G3 arraying robot after being transferred manually into three 384-well plates.Meanwhile 2501 and 2890 clones of Library in pYLTAC17 and in pYLTAC747H/sacB were stored individually in fourteen 384-well plates and then were automatically gridding in duplicate onto a high-density colony hybridization filter with a 6×6 pattern after a replication of plates.Nineteen positive clones were detected by using the probe glutahione reductase gene of L.multicaulis.TAC libraries constructed here can be used to isolate genomic clones containing target genes,and to carry out genome walking for positional cloning.Once the target TAC clones were isolated,they could be immediately transferred into plant genomes with the Agrobacterium system. | XU YueYu 1,2,3,ZHOU YuLei 4,5 ,SONG LinLin 6 ,ZHANG Yan 3 &ZHAO MaoLin 1 1 Beijing Agro-Biotechnology Research Center,Beijing Academy of Agriculture and Forestry Science,Beijing 100097,China 2 Department of Chemistry and Chemical Engineering,Hunan Institute of Engineering,Xiangtan 411104,China 3 College of Life Science,Capital Normal University,Beijing 100037,China 4 College of Life Science,Zhongkai University of Agriculture and Technology,Guangzhou 510225,China 5 College of Grassland Science,Gansu Agricultural University,Lanzhou 730070,China 6 Department of Biology,Henan Institute of Science and Technology,Xinxiang 453003,China | 2008 | Science China(Life Sciences)2008，51，7： | 1 |
| 18 | Empirical Analysis of Factors Influencing Compensation for Non-commercial Forests under CVM显示文摘Based on non-commercial forests in Kunming City,this article conducts survey of 506 households in Kunming City to evaluate their willingness to pay ecological benefit of non-commercial forests,using Contingent Valuation Method(CVM);to analyze factors influencing their willingness to compensate for non-commercial forests,using multivariate statistical analysis method.The results show that income,educational level, housing ownership,outdoor exercise time,whether to be concerned about environmental problems,frequency of obtaining forest-related information monthly and whether understanding non-commercial forests or not,are the most principal factors influencing willingness to pay. | ZHANG Mei College of Economics and Trade,Zhongkai University of Agriculture and Engineering,Guangzhou 510225,China | 2012 | Asian Agricultural Research2012，4，7： | 1 |
| 19 | Differences in properties of collagen, gelatin and collagen hydrolysate 显示文摘 | Li Guoying Zhang Zhongkai Lei Su | 2005 | Journal of Sichuan University: Engineer- ing Science Edition2005，37，4： | 1 |
| 20 | TURBULENT STRUCTURE AND LOCAL SIMILARITY IN THE TOWER LAYER OVER THE NANJING AREA显示文摘 | XU YUMAO ZHOU CHAOFU LI ZHONGKAI ZHANG WEI | 1997 | Boundary - Layer Meteorology1997，，1： | 1 |
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