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| 1 | Tobacco curly shoot virus isolated in Yunnan is a distinct species of Begomovirus显示文摘Virus isolate Y1 was obtained from tobacco showing curly shoot symptoms in Baoshan, Yunnan Province. Whitefly transmission test and virion morphology observa-tion showed that it is a begomovirus. In reactions with 14 monoclonal antibodies raised against begomoviruses, Y1 was readily differentiated from begomoviruses reported in China, Pakistan and India. The complete nucleotide sequence of DNA-A was determined, it contains 2746 nucleotides, with two ORFs in virion-sense DNA and four ORFs in comple-mentary-sense DNA. Comparisons with total DNA-A, inter-genie region and deduced amino acid sequences of individual ORFs showed that Y1 is a distinct Begomovirus species, for which the name Tobacco curly shoot virus (TCSV) is pro-posed. The total DNA-A of TCSV is most closely related to that of Tomato leaf curl virus from India (85% sequence identity). In contrast, the deduced coat protein of TCSV is most like that of Cotton leaf curl virus 72b isolate from Paki-stan (98% amino acid sequence identity). | XIE Yan ZHOU Xueping ZHANG Zhongkai QI Yijun | 2002 | Chinese Science Bulletin2002，47，3： | 51 |
| 2 | Dense ceramics with complex shape fabricated by 3D printing:A review显示文摘Three-dimensional(3D)printing technology is becoming a promising method for fabricating highly complex ceramics owing to the arbitrary design and the infinite combination of materials.Insufficient density is one of the main problems with 3D printed ceramics,but concentrated descriptions of making dense ceramics are scarce.This review specifically introduces the principles of the four 3D printing technologies and focuses on the parameters of each technology that affect the densification of 3D printed ceramics,such as the performance of raw materials and the interaction between energy and materials.The technical challenges and suggestions about how to achieve higher ceramic density are presented subsequently.The goal of the presented work is to comprehend the roles of critical parameters in the subsequent 3D printing process to prepare dense ceramics that can meet the practical applications. | Zhe Chen Xiaohong Sun Yunpeng Shang Kunzhou Xiong Zhongkai Xu Ruisong Guo Shu Cai Chunming Zheng | 2021 | Journal of Advanced Ceramics2021，10，2： | 10 |
| 3 | Sulfonated polyaniline/vanadate composite as anode material and its electrochemical property in microbial fuel cells on ocean floor显示文摘A unique sulfonated polyaniline/vanadate composite was synthesized and utilized as a composite anode in microbial fuel cells on ocean floor (BMFCs). X-ray diffraction (XRD) and thermogravimetric analysis (TGA) were employed to characterize its chemical composition and morphology. Wettability of the composite anodes decreases due to the addition of polytetrafluoroethylene (PTFE). The electrochemical behavior of the composite anodes was investigated by means of linear sweep voltammetry and Tafel plot measurements. Compared with the plain graphite anode,the composite anode significantly improves the power density,5.5-fold higher,reaching 187.1 mW/m2 and gives a 27-fold higher exchange current density and a higher kinetic activity. A novel synergistic mechanism between sulfonated polyaniline and vanadate is proposed to explain the excellent electrochemical performance. This composite thus has great potential to be used as an anode material for a high-power BMFC. | FU YuBin ZHAO ZhongKai LIU Jia LI KuiZhong XU Qian ZHANG ShaoYun | 2011 | Science China Chemistry2011，54，5： | 8 |
| 4 | Purification and Characterization of a New Ribosome Inactivating Protein from Cinchonaglycoside C-treated Tobacco Leaves显示文摘A new ribosome-inactivating protein(RIP)with a molecular weight of 31 kDa induced by Cinchonaglycoside C(1)designatedCLP31,was isolated from tobacco leaves.Analysis of this protein sequence indicated that it belongs to the RIP family and itwas distinct from the other plant RIPs reported previously at its N-terminal amino acid sequence.CIP31 can directly impairsynthesis of coat protein(CP)of tobacco mosaic virus(TMV),which resulted in inhibition of TMV long distance movementand multiplication in tobacco plants at concentrations of ng/mL.Furthermore,no toxicity was shown to the growth andfertility of the plants.CIP31 was synthesized only in the presence of Cinchonaglycoside C(1)and was independent of thesalicylic acid(SA)signal pathway.We provided evidence for the SA-independent biological induction of resistance. | Yanmei Li Yantao Jia Zhongkai Zhang Xiaoying Chen Hongping He Rongxiang Fang Xiaojiang Hao | 2007 | Journal of Integrative Plant Biology2007，49，9： | 8 |
| 5 | Habitat-induced reciprocal transformation in the root phenotype of Oriental ginseng is associated with alteration in DNA methylation显示文摘Oriental ginseng is an important medicinal plant that grows in 2 major forms or ecotypes,wild and domesticated.Each form differs conspicuously in root phenotype,but can be converted from one type to another by habitat.Here we show that the habi tat-induced transformation of ginseng root phenotype was accompanied by alteration in cytosine methylation at a large number of 5′-CCGG-3′sites detected by the methylation-sensitive polymorphism(MSAP)marker.The collective CG and CHG methylation levels of all 4 landraces of the domesticated form were significantly lower than those of the wild form.Interestingly,artificially transplanted ginseng plants recreated in both directions the methylation levels(at least in CHG)of their natural counterparts.The methylation differences between the 2 ginseng ecotypes were validated at 2 isolated MSAP loci bearing homology to a 5S rRNA gene or a copia retrotransposon.Our results implicate a link between epigenetic variation and habitat-induced phenotypic flexibility in Oriental ginseng. | NGEZAHAYO Frederic WANG XiaoLi YU XiaoMing JIANG LiLi CHU YunJie SHEN BaiHua YAN ZhongKai LIU Bao | 2011 | Chinese Science Bulletin2011，56，16： | 7 |
| 6 | Isorhapontigenin protects against doxorubicin-induced cardiotoxicity via increasing YAP1 expression显示文摘As an effective anticancer drug, the clinical limitation of doxorubicin(Dox) is the time-and dose-dependent cardiotoxicity. Yes-associated protein 1(YAP1) interacts with transcription factor TEA domain 1(TEAD1) and plays an important role in cell proliferation and survival. However, the role of YAP1 in Dox-induced cardiomyopathy has not been reported. In this study, the expression of YAP1 was reduced in clinical human failing hearts with dilated cardiomyopathy and Dox-induced in vivo and in vitro cardiotoxic model. Ectopic expression of Yap1 significantly blocked Dox-induced cardiomyocytes apoptosis in TEAD1 dependent manner. Isorhapontigenin(Isor) is a new derivative of stilbene and responsible for a wide range of biological processes. Here, we found that Isor effectively relieved Doxinduced cardiomyocytes apoptosis in a dose-dependent manner in vitro. Administration with Isor(30 mg/kg/day, intraperitoneally, 3 weeks) significantly protected against Dox-induced cardiotoxicity in mice. Interestingly, Isor increased Dox-caused repression in YAP1 and the expression of its target genes in vivo and in vitro. Knockout or inhibition of Yap1 blocked the protective effects of Isor on Dox-induced cardiotoxicity. In conclusion, YAP1 may be a novel target for Dox-induced cardiotoxicity and Isor might be a new compound to fight against Dox-induced cardiotoxicity by increasing YAP1 expression. | Panxia Wang Minghui Wang Yuehuai Hu Jianxing Chen Yanjun Cao Cui Liu Zhongkai Wu Juan Shen Jing Lu Peiqing Liu | 2021 | Acta Pharmaceutica Sinica B2021，11，3： | 7 |
| 7 | Identification of a novel DNA molecule associated with Tobacco leaf curl virus显示文摘A circular DNA molecule, designated as DNAp, was identified in tobacco plants infected with Tobacco leaf curl virus (TLCV) isolates Y5 and Y8 by PCR using primers based on the conserved region of the two reported DNAp sequences of whitefly-transmitted geminiviruses (WTGs). The complete nucleotide sequences of DNAβ of Y5 and Y8 (TLCV DNAβ) were determined. Y5 DNAβ comprises 1333 nucleotides encoding 8 predicted ORFs with 4 ORFs in virion-sense DNA and 4 ORFs in complementary-sense DNA; Y8 DNAP consists of 1338 nucleotides encoding 7 predicted ORFs with 4 ORFs in virion-sense DNA and 3 ORFs in complementary-sense DNA. TLCV DNAβhas little sequence homology to DNA-A of TLCV, except that it shares conserved TAATATTAC loop sequence with TLCV DNA-A. Sequence comparison showed that Y5 DNAβ shared 85% sequence homology with Y8 DNAβ, and both Y5 DNAβ and Y8 DNAβ had relatively low sequence identity (51%-65%) with the reported DNAβ molecules associated with Agera-tum yellow vein virus and Cotton leaf | XIE Yan ZHOU Xueping LI Zhenghe ZHANG Zhongkai LI Guixin | 2002 | Chinese Science Bulletin2002，47，15： | 6 |
| 8 | Trace Fossils from Lower Cambrian Wusongger Formation in Xiaoerbulake Outcrop,Kalpin Area,Xinjiang显示文摘Based on the analysis of trace fossils collected from the typical outcrop of the Lower Cambrian Wusongger Formation in the Kalpin area,ten ichnospecies of six ichnogenus were identified in the upper member of the Wusongger Formation,with most of them being found for the first time.The trace fossils are described seriatim.The ichnospecies are mainly represented by such common trace fossils as Ophiomorpha nodosa,Helminthopsis hieroglyphica,Helminthopsis ichnosp.,Planolites beverleyensis,Planolites vulgaris,Planolites montanus,Palaeophycus striatus,Palaeophycus curvatus?,Cochlichnus anguineus and Rituichnus elongatum.The trace fossils are of high diversity and low abundance,and can be diagnosed as the Cruziana ichnofacies.They can be interpreted as having formed in a shallow water environment. | BAI Zhongkai LU Xiuxiang LIU Xiaoping XIE Qilai LI Jianjiao WU Jianjun | 2012 | Acta Geologica Sinica(English Edition)2012，86，2： | 6 |
| 9 | Antiskid Control of Railway Train Braking Based on Adhesion Creep Behavior显示文摘In modern trains wheelset skidding leads to the deterioration of braking behavior,the degradation of comfort,as well as a boost in system hazards.Because of the nonlinearity and unknown characteristics of wheelset adhesion,simplifications are widely adopted in the modeling process of conventional antiskid controllers.Therefore,conventional antiskid controllers usually cannot perform satisfactorily.In this paper,systematic computer simulation and field tests for railway antiskid control system are introduced.The operating principal of antiskid control system is explained,which is fundamental to the simulation of antiskid brakes,and the simulation model is introduced,which incorporates both the adhesion creep curve and a pneumatic submodel of antiskid control system.In addition,the characteristics of adhesion curves and the simulation target are also provided.Using DHSplus,the pneumatic submodel is created to analyze the performance of the different control strategies of antiskid valves.Then the system simulation is realized by combining the kinematical characteristics of railway trains and the pneumatic submodel.The simulation is performed iteratively to obtain the optimized design of the antiskid control system.The design result is incorporated in the hardware design of the antiskid control system and is evaluated in the field tests in Shanghai Subway Line 1.Judging by the antiskid efficiency,the antiskid braking performance observed in the field tests shows the superiority of the optimized design.Therefore,the proposed simulation method,especially in view of its ease of application,appears to be a useful one for designing railway antiskid control systems. | ZUO Jianyong CHEN Zhongkai | 2012 | Chinese Journal of Mechanical Engineering2012，25，3： | 6 |
| 10 | METHOD FOR PRECISE CALCULATION OF ROOT STRESS OF SPIRAL BEVEL GEARS显示文摘METHODFORPRECISECALCULATIONOFROOTSTRESSOFSPIRALBEVELGEARSMETHODFORPRECISECALCULATIONOFROOTSTRESSOFSPIRALBEVELGEARSChenLiangyu... | Chen Liangyu Wang Yanzhong Zheng Xijian E Zhongkai Cai Chunyuan(Northeastern University) | 1994 | Chinese Journal of Mechanical Engineering1994，7，4： | 5 |
| 11 | Fragility assessment of tunnels in soft soils using artificial neural networks显示文摘Recent earthquakes have shown that tunnels are prone to damage,posing a major threat to safety and having major cascading and socioeconomic impacts.Therefore,reliable models are needed for the seismic fragility assessment of underground structures and the quantitative evaluation of expected losses.Based on previous researches,this paper presented a probabilistic framework based on an artificial neural network(ANN),aiming at the development of fragility curves for circular tunnels in soft soils.Initially,a two-dimensional incremental dynamic analysis of the nonlinear soil-tunnel system was performed to estimate the response of the tunnel under ground shaking.The effects of soil-structure-interaction and the ground motion characteristics on the seismic response and the fragility of tunnels were adequately considered within the proposed framework.An ANN was employed to develop a probabilistic seismic demand model,and its results were compared with the traditional linear regression models.Fragility curves were generated for various damage states,accounting for the associated uncertainties.The results indicate that the proposed ANN-based probabilistic framework can results in reliable fragility models,having similar capabilities as the traditional approaches,and a lower computational cost is required.The proposed fragility models can be adopted for the risk analysis of typical circular tunnel in soft soils subjected to seismic loading,and they are expected to facilitate decision-making and risk management toward more resilient transport infrastructure. | Zhongkai Huang Sotirios A.Argyroudis Kyriazis Pitilakis Dongmei Zhang Grigorios Tsinidis | 2022 | Underground Space2022，7，2： | 4 |
| 12 | Sensor Configuration and Test for Fault Diagnoses of Subway Braking System Based on Signed Digraph Method显示文摘Fault diagnosis of various systems on rolling stock has drawn the attention of many researchers.However,obtaining an optimized sensor set of these systems,which is a prerequisite for fault diagnosis,remains a major challenge.Available literature suggests that the configuration of sensors in these systems is presently dependent on the knowledge and engineering experiences of designers,which may lead to insufficient or redundant development of various sensors.In this paper,the optimization of sensor sets is addressed by using the signed digraph(SDG)method.The method is modified for use in braking systems by the introduction of an effect-function method to replace the traditional quantitative methods.Two criteria are adopted to evaluate the capability of the sensor sets,namely,observability and resolution.The sensors configuration method of braking system is proposed.It consists of generating bipartite graphs from SDG models and then solving the set cover problem using a greedy algorithm.To demonstrate the improvement,the sensor configuration of the HP2008 braking system is investigated and fault diagnosis on a test bench is performed.The test results show that SDG algorithm can improve single-fault resolution from 6 faults to 10 faults,and with additional four brake cylinder pressure(BCP)sensors it can cover up to 67 double faults which were not considered by traditional fault diagnosis system.SDG methods are suitable for reducing redundant sensors and that the sensor sets thereby obtained are capable of detecting typical faults,such as the failure of a release valve.This study investigates the formal extension of the SDG method to the sensor configuration of braking system,as well as the adaptation supported by the effect-function method. | ZUO Jianyong CHEN Zhongkai | 2014 | Chinese Journal of Mechanical Engineering2014，27，3： | 4 |
| 13 | Electrochemical Properties of Electrodes with Different Shapes and Diffusion Kinetic Analysis of Microbial Fuel Cells on Ocean Floor显示文摘Microbial fuel cell(MFC) on the ocean floor is a kind of novel energy-harvesting device that can be developed to drive small instruments to work continuously.The shape of electrode has a great effect on the performance of the MFC.In this paper,several shapes of electrode and cell structure were designed,and their performance in MFC were compared in pairs:Mesh(cell-1) vs.flat plate(cell-2),branch(cell-3) vs.cylinder(cell-4),and forest(cell-5) vs.disk(cell-6) FC.Our results showed that the maximum power densities were 16.50,14.20,19.30,15.00,14.64,and 9.95 mWm-2 for cell-1,2,3,4,5 and 6 respectively.And the corre-sponding diffusion-limited currents were 7.16,2.80,18.86,10.50,18.00,and 6.900 mA.The mesh and branch anodes showed higher power densities and much higher diffusion-limited currents than the flat plate and the cylinder anodes respectively due to the low diffusion hindrance with the former anodes.The forest cathode improved by 47% of the power density and by 161% of diffusion-limited current than the disk cathode due to the former's extended solid/liquid/gas three-phase boundary.These results indicated that the shape of electrode is a major parameter that determining the diffusion-limited current of an MFC,and the differences in the elec-trode shape lead to the differences in cell performance.These results would be useful for MFC structure design in practical applica-tions. | FU Yubin LIU Jia SU Jia ZHAO Zhongkai LIU Yang XU Qian | 2012 | Journal of Ocean University of China2012，11，1： | 3 |
| 14 | Distribution Features of the Nanhua-Sinian Rifts and their Significance to Hydrocarbon Accumulation in the Tarim Basin显示文摘On the basis of reprocessing 34 new two-dimensional spliced long sections(20,191 km) in the Tarim Basin, the deep structure features of the Tarim Basin were analyzed through interpreting 30,451 km of two-dimensional seismic data and compiling basic maps. Seismic interpretation and geological analysis conclude that the Nanhua-Sinian strata are a set of rift-depression depositional systems according to their tectonic and depositional features. The rift valley formed in the Nanhua Period, and the transformation became weaker during the late Sinian Period, which eventually turned into depression. From bottom to top, the deposited strata include mafic igneous, tillite, mudstone, and dolomite. Three major depocenters developed inside this basin during the rift stage and are distributed in the eastern Tarim Basin, the Awati area, and the southwestern Tarim Basin. Among them, the rift in the eastern Tarim Basin strikes in the near east-west direction on the plane and coincides with the aeromagnetic anomaly belt. This represents a strong magnetic zone formed by upwelling basic volcanic rock along high, steep normal faults of the Nanhua Period. Controlled by the tectonic background, two types of sedimentary systems were developed in the rift stage and depression stage, showing two types of sequence features in the Sinian depositional stage. The Nanhua System appears as a wedge-shaped formation, with its bottom in unconformable contact with the base. The rifting event has a strong influence on the current tectonic units in the Tarim Basin, and affects the distribution of source rock in the Yuertus Formation and reservoir beds in the Xiaoerbulake Formation in Lower Cambrian, as well as the gypseous cap rock in Middle Cambrian. The distribution features of the rifts have important and realistic significance for determining the direction of oil and gas exploration in the deep strata of the Tarim Basin. Comprehensive analysis suggests that the Tazhong region is the most favorable zone, and the Kalpin-Bachu region is the optimal potential zone for exploring sub-salt oil and gas in deep Cambrian strata. | BAI Zhongkai CHENG Minghua CHEN Cheng HUO Junzhou ZHANG Junfeng ZHOU Xingui GAO Yongjin ZHAO Bo QIU Haijun HAN Miao YANG Youxing SUN Zhichao MIAO Miaoqing ZHANG Jinhu LI Qingyao | 2020 | Acta Geologica Sinica(English Edition)2020，94，2： | 3 |
| 15 | Inflation analyses of a circular elastomeric membrane with hydrostatic pressure显示文摘Composite diaphragm inflation(CDI) experiment is a useful methodology for exploring the mechanics relation of cell-cell junctions within a living epithelial sheet.In this paper,a new methodology of CDI experiment has been put forward.The finite deformation of a circular polydimezhylsiloxane elastomeric membrane,which is used as the substrate for the culture of epithelial cells,under hydrostatic pressure is explored by the theory of non-linear continuum mechanics.The simplified governing equations with minimum amount of differential equations and boundary conditions are obtained and solved by multiple shooting method.The influences on the membrane by hydrostatic pressure have been discussed. | Zhongkai Zhang Jianbing Sang Yunjiao Zhang | 2012 | Theoretical & Applied Mechanics Letters2012，2，6： | 3 |
| 16 | FORMING PROCESS OF HOT-EXTRUDED SiCw/6061Al COMPOSITES(Ⅰ)显示文摘1INTRODUCTIONInrecentyears,moreandmorecontinuouslyordiscontinuouslyreinforcedmetalmatrixcomposites(MMCs)havebeenusedtomakest... | Zhang Wenlong, Cai Liuchun, Peng Huaxin, Wang Dezun and Yao Zhongkai School of Materials Science and Engineering, Harbin Institute of Technology, Harbin 150001, P. R. China | 1998 | 中国有色金属学会会刊:英文版1998，8，3： | 3 |
| 17 | An Iterative Learning Approach to Identify Fractional Order KiBaM Model显示文摘This paper discusses the parameter and differentiation order identification of continuous fractional order Ki Ba M models in ARX(autoregressive model with exogenous inputs)and OE(output error model) forms. The least squares method is applied to the identification of nonlinear and linear parameters,in which the Grunwald-Letnikov definition and short memory principle are applied to compute the fractional order derivatives.An adaptive P-type order learning law is proposed to estimate the differentiation order iteratively and accurately. Particularly,a unique estimation result and a fast convergence speed can be arrived by using the small gain strategy, which is unidirectional and has certain advantages than some state-of-art methods. The proposed strategy can be successfully applied to the nonlinear systems with quasi-linear characteristics. The numerical simulations are shown to validate the concepts. | Yang Zhao Yan Li Fengyu Zhou Zhongkai Zhou YangQuan Chen | 2017 | IEEE/CAA Journal of Automatica Sinica2017，4，2： | 2 |
| 18 | Modification of Nano Tourmaline Surface Treatment Agent and Its Performance on Negative Ion Release显示文摘In this paper,a kind of wall fabric’s surface treatment agent modified with nonionic surfactant was reported.This surface treatment agent was prepared by using nano tourmaline powder dispersion in water with surfactant as dispersants by sand milling.Under the influence of different dispersants,the negative ions releasing amount of functional wall fabrics,the milling process and the storage stability of nano tourmaline powder dispersion were discussed.The results showed that nano tourmaline powder dispersion achieved the smallest average diameter of 44 nm and had best storage stability that the average diameter maintained below 200 nm in 17 days when the addition amount of dispersant was 20 percent of the tourmaline powders’weight.What is more,the quantity of negative ion releasing achieved 6500 ion/cm3 when addition amount of dispersant was 30 percent.This technique could be used to strengthen productivity of nano tourmaline powder dispersion. | Guorui Huang Zhongkai Cui Pengfei Zhu Xiaoyun Liu | 2018 | Computers, Materials & Continua2018，，10： | 2 |
| 19 | AN EMPIRICAL STUDY ON USER ACCESS TO ENTERTAINMENT STREAMING MEDIA显示文摘Due to large size and different popularity for different part of the video, most proxy caches for streaming medias cache only a part of the video. Thus, an accurate understanding on the internal popularity distribution of media objects in streaming applications is very important for the development of efficient cache mechanisms. This letter shows that the internal popularity of popular streaming media obeys a k-transformed Zipf-like distribution through analyzing two 6-month long traces recorded at different streaming video servers of an entertainment video-on-demand provider. This empirical model can be used to design an efficient cach- ing algorithm. | Yu Jiang Chou Chun Tung Yang Zhongkai Du Xu Wang Tai | 2006 | Journal of Electronics(China)2006，23，5： | 2 |
| 20 | Optimal Pricing and Advertising Com-petition in Two Supply Chains with Determnistic Demand显示文摘 | Jiajia Nie | 2007 | In-ternational joumal of manufacturjng te chnology and management science2007，，： | 1 |
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