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| 1 | ALK,the Key Gene for Gelatinization Temperature,is a Modifier Gene for Gel Consistency in Rice显示文摘Gelatinization temperature(GT) is an important parameter in evaluating the cooking and eating quality of rice.Indeed,the phenotype,biochemistry and inheritance of GT have been widely studied in recent times.Previous map-based cloning revealed that GT was controlled by ALK gene,which encodes a putative soluble starch synthase II-3.Complementation vector and RNAi vector were constructed and transformed into Nipponbare mediated by Agrobacterium.Phenotypic and molecular analyses of transgenic lines provided direct evidence for ALK as a key gene for GT.Meanwhile,amylose content,gel consistency and pasting properties were also affected in transgenic lines.Two of four nonsynonymous single nucleotide polymorphisms in coding sequence of ALK were identified as essential for GT.Based on the single nucleotide polymorphisms(SNPs),two new sets of SNP markers combined with one cleaved amplified polymorphic sequence marker were developed for application in rice quality breeding. | Zhenyu Gao Dali Zeng Fangmin Cheng Zhixi Tian Longbiao Guo Yan Su Meixian Yan Hua Jiang Guojun Dong Yuchen Huang Bin Han Jiayang Li Qian Qian | 2011 | Journal of Integrative Plant Biology2011，53，9： | 13 |
| 2 | Global Dynamic Molecular Profiling of Stomatal Lineage Cell Development by Single-Cell RNA Sequencing显示文摘The regulation of stomatal lineage cell development has been extensively investigated.However,a comprehensive characterization of this biological process based on single-cell transcriptome analysis has not yet been reported.In this study,we performed RNA sequencing on 12844 individual cells from the cotyledons of 5-day-old Arabidopsis seedlings.We identified 11 cell clusters corresponding mostly to cells at specific stomatal developmental stages using a series of marker genes.Comparative analysis of genes with the highest variable expression among these cell clusters revealed transcriptional networks that regulate development from meristemoid mother cells to guard mother cells.Examination of the developmental dynamics of marker genes via pseudo-time analysis revealed potential interactions between these genes.Collectively,our study opens the door for understanding how the identified novel marker genes participate in the regulation of stomatal lineage cell development. | Zhixin Liu Yaping Zhou Jinggong Guo Jiaoai Li Zixia Tian Zhinan Zhu Jiajing Wang Rui Wu Bo Zhang Yongjian Hu Yijing Sun Yan Shangguan Weiqiang Li Tao Li Yunhe Hu Chenxi Guo Jean-David Rochaix Yuchen Miao Xuwu Sun | 2020 | Molecular Plant2020，13，8： | 11 |
| 3 | Optimizing the CRISPR/Cas9 system for genome editing in grape by using grape promoters显示文摘The efficacy of the CRISPR/Cas9 system in grapevine(Vitis vinifera L.)has been documented,but the optimization of this system,as well as CRISPR/Cas9-mediated multiplex genome editing,has not been explored in this species.Herein,we identified four VvU3 and VvU6 promoters and two ubiquitin(UBQ)promoters in grapevine and demonstrated that the use of the identified VvU3/U6 and UBQ2 promoters could significantly increase the editing efficiency in grape by improving the expression of sgRNA and Cas9,respectively.Furthermore,we conducted multiplex genome editing using the optimized CRISPR/Cas9 vector that contained the conventional multiple sgRNA expression cassettes or the polycistronic tRNA-sgRNA cassette(PTG)by targeting the sugar-related tonoplastic monosaccharide transporter(TMT)family members TMT1 and TMT2,and the overall editing efficiencies were higher than 10%.The simultaneous editing of TMT1 and TMT2 resulted in reduced sugar levels,which indicated the role of these two genes in sugar accumulation in grapes.Moreover,the activities of the VvU3,VvU6,and UBQ2 promoters in tobacco genome editing were demonstrated by editing the phytoene desaturase(PDS)gene in Nicotiana benthamiana leaves.Our study provides materials for the optimization of the CRISPR/Cas9 system.To our knowledge,our simultaneous editing of the grape TMT family genes TMT1 and TMT2 constitutes the first example of multiplex genome editing in grape.The multiplex editing systems described in this manuscript expand the toolbox of grape genome editing,which would facilitate basic research and molecular breeding in grapevine. | Chong Ren Yanfei Liu Yuchen Guo Wei Duan Peige Fan Shaohua Li Zhenchang Liang | 2021 | Horticulture Research2021，8，1： | 6 |
| 4 | From Brain Science to Artificial Intelligence显示文摘Reviewing the history of the development of artificial intelligence(AI)clearly reveals that brain science has resulted in breakthroughs in AI,such as deep learning.At present,although the developmental trend in AI and its applications has surpassed expectations,an insurmountable gap remains between AI and human intelligence.It is urgent to establish a bridge between brain science and AI research,including a link from brain science to AI,and a connection from knowing the brain to simulating the brain.The first steps toward this goal are to explore the secrets of brain science by studying new brain-imaging technology;to establish a dynamic connection diagram of the brain;and to integrate neuroscience experiments with theory,models,and statistics.Based on these steps,a new generation of AI theory and methods can be studied,and a subversive model and working mode from machine perception and learning to machine thinking and decision-making can be established.This article discusses the opportunities and challenges of adapting brain science to AI. | Jingtao Fan Lu Fang Jiamin Wu Yuchen Guo Qionghai Dai | 2020 | Engineering2020，6，3： | 4 |
| 5 | Analog Optical Computing for Artificial Intelligence显示文摘The rapid development of artificial intelligence(AI)facilitates various applications from all areas but also poses great challenges in its hardware implementation in terms of speed and energy because of the explosive growth of data.Optical computing provides a distinctive perspective to address this bottleneck by harnessing the unique properties of photons including broad bandwidth,low latency,and high energy efficiency.In this review,we introduce the latest developments of optical computing for different AI models,including feedforward neural networks,reservoir computing,and spiking neural networks(SNNs).Recent progress in integrated photonic devices,combined with the rise of AI,provides a great opportunity for the renaissance of optical computing in practical applications.This effort requires multidisciplinary efforts from a broad community.This review provides an overview of the state-of-the-art accomplishments in recent years,discusses the availability of current technologies,and points out various remaining challenges in different aspects to push the frontier.We anticipate that the era of large-scale integrated photonics processors will soon arrive for practical AI applications in the form of hybrid optoelectronic frameworks. | Jiamin Wu Xing Lin Yuchen Guo Junwei Liu Lu Fang Shuming Jiao Qionghai Dai | 2022 | Engineering2022，8，3： | 4 |
| 6 | A male-ABCD algorithm for hepatocellular carcinoma risk prediction in HBs Ag carriers显示文摘Objective: Hepatocellular carcinoma(HCC) development among hepatitis B surface antigen(HBs Ag) carriers shows gender disparity, influenced by underlying liver diseases that display variations in laboratory tests. We aimed to construct a risk-stratified HCC prediction model for HBs Ag-positive male adults.Methods: HBs Ag-positive males of 35-69 years old(N=6,153) were included from a multi-center populationbased liver cancer screening study. Randomly, three centers were set as training, the other three centers as validation. Within 2 years since initiation, we administrated at least two rounds of HCC screening using Bultrasonography and α-fetoprotein(AFP). We used logistic regression models to determine potential risk factors,built and examined the operating characteristics of a point-based algorithm for HCC risk prediction.Results: With 2 years of follow-up, 302 HCC cases were diagnosed. A male-ABCD algorithm was constructed including participant's age, blood levels of GGT(γ-glutamyl-transpeptidase), counts of platelets, white cells,concentration of DCP(des-γ-carboxy-prothrombin) and AFP, with scores ranging from 0 to 18.3. The area under receiver operating characteristic was 0.91(0.90-0.93), larger than existing models. At 1.5 points of risk score,26.10% of the participants in training cohort and 14.94% in validation cohort were recognized at low risk, with sensitivity of identifying HCC remained 100%. At 2.5 points, 46.51% of the participants in training cohort and 33.68% in validation cohort were recognized at low risk with 99.06% and 97.78% of sensitivity, respectively. At 4.5 points, only 20.86% of participants in training cohort and 23.73% in validation cohort were recognized at high risk,with positive prediction value of 22.85% and 12.35%, respectively.Conclusions: Male-ABCD algorithm identified individual's risk for HCC occurrence within short term for their HCC precision surveillance. | Yuting Wang Minjie Wang He Li Kun Chen Hongmei Zeng Xinyu Bi Zheng Zhu Yuchen Jiao Yong Wang Jian Zhu Hui Zhao Xiang Liu Chunyun Dai Chunsun Fan Can Zhao Deyin Guo Hong Zhao Jianguo Zhou Dongmei Wang Zhiyuan Wu Xinming Zhao Wei Cui Xuehong Zhang Jianqiang Cai Wanqing Chen Chunfeng Qu | 2021 | Chinese Journal of Cancer Research2021，33，3： | 3 |
| 7 | A CRISPR-Cas9 screen shows the combination efficacy of lenvatinib plus epidermal growth factor receptor inhibitors for treatment of liver cancer显示文摘Recently,in cooperation with the Netherlands Cancer Institute,we demonstrated that epidermal growth factor receptor(EGFR)activation limited the response of liver cancer to lenvatinib.The original article was published in Nature as a cover story,which resulted in considerable attention from major scientific journals.Here,we were invited by Cancer Biology&Medicine to provide comments on preclinical and clinical findings about the combination of lenvatinib plus EGFR inhibitors as a promising strategy for hepatocellular carcinoma(HCC)treatment.Primary liver cancer represents the sixth most common malignancy and the third leading cause of cancer-related mortality worldwide,with an estimated 906,000 new cases and 830,000 deaths in 20201. | Yuchen Guo Yangyang Zhou Wenxin Qin | 2022 | Cancer Biology & Medicine2022，19，2： | 2 |
| 8 | Evaluation of the weakening behavior of gas on the coal strength and its quantitative influence on the coal deformation显示文摘The coal strength and deformation properties are key factors affecting safe coal mining and highefficiency coalbed methane(CBM)development.In this paper,reconstituted coal samples are chosen to investigate the weakening behavior of gas on coal strength,meanwhile,its effects on coal deformation are quantitatively evaluated.The results indicate that the weakening degree of gas on coal strength is closely related to the confining stress and gas pressure.Compared with non-gas-saturated coals,the maximum weakening ratios of adsorbed gas to coal strength are 10.58%,18.12%,8.55%and 14.65%under the conditions of confining stress CS=3 MPa and gas pressure GP=1 MPa,CS=3 MPa and GP=2 MPa,CS=4 MPa and GP=1 MPa,and CS=4 MPa and GP=2 MPa,respectively.Furthermore,the maximum weakening ratios of free gas to coal strength are 18.27%,36.54%,14.79%and 29.58%,respectively,under above four conditions.The maximum coal bulk strain decreases as particle sizes of coal powders increase,and it has a maximum value of 0.0227 and a minimum value of 0.0191 in particle size ranges of 0.01–0.041 and 0.5–1 mm.Under the same conditions,the coal bulk strain increases with increasing gas pressure,revealing that coal deformation properties can be enhanced by gas. | Haijun Guo Kai Wang Yuchen Wu Hanlu Tang Jianguo Wu Lianhe Guan Chenyang Chang Chao Xu | 2021 | International Journal of Mining Science and Technology2021，31，3： | 2 |
| 9 | Osmotically stress-regulated the expression of glutathione peroxidase 3 in Arabidopsis显示文摘Gene expression of glutathione peroxidase 3 (ATGPX3) in response to osmotic stress was analyzed in Arabidopsis using ATGPX3 promoter-glucuronidase (GUS) transgenic plants. High levels of GUS ex- pression were detected under osmotic stress in ATGPX3 promoter-GUS transgenic plants. Compared with the wild type, the growth and development of ATGPX3 mutants (atgpx3-1) were more sensitive to mannitol. In addition, the expression of RD29A, ABI1, ABI2 and RbohD in atgpx3-1 was induced by ABA stress. These results suggest that ATGPX3 might be involved in the signal transduction of osmotic stress. | MIAO YuChen GUO JingGong LIU ErTao LI Kun DAI Jie WANG PengCheng CHEN Jia SONG ChunPeng | 2007 | Chinese Science Bulletin2007，52，1： | 1 |
| 10 | A Survey of Human Action Recognition and Posture Prediction显示文摘Human action recognition and posture prediction aim to recognize and predict respectively the action and postures of persons in videos.They are both active research topics in computer vision community,which have attracted considerable attention from academia and industry.They are also the precondition for intelligent interaction and human-computer cooperation,and they help the machine perceive the external environment.In the past decade,tremendous progress has been made in the field,especially after the emergence of deep learning technologies.Hence,it is necessary to make a comprehensive review of recent developments.In this paper,firstly,we attempt to present the background,and then discuss research progresses.Secondly,we introduce datasets,various typical feature representation methods,and explore advanced human action recognition and posture prediction algorithms.Finally,facing the challenges in the field,this paper puts forward the research focus,and introduces the importance of action recognition and posture prediction by taking interactive cognition in self-driving vehicle as an example. | Nan Ma Zhixuan Wu Yiu-ming Cheung Yuchen Guo Yue Gao Jiahong Li Beiyan Jiang | 2022 | Tsinghua Science and Technology2022，27，6： | 1 |
| 11 | Targeting the brain with PEG–PLGA nanoparticles modified with phage-displayed peptides显示文摘 | Jingwei Li Liang Feng Li Fan Yuan Zha Liangran Guo Qizhi Zhang Jun Chen Zhiqing Pang Yuchen Wang Xinguo Jiang Victor C. Yang Longping Wen | 2011 | Biomaterials2011，，21： | 1 |
| 12 | Targeting the brain with PEG–PLGA nanoparticles modified with phage-displayed peptides显示文摘 | Jingwei Li Liang Feng Li Fan Yuan Zha Liangran Guo Qizhi Zhang Jun Chen Zhiqing Pang Yuchen Wang Xinguo Jiang Victor C. Yang Longping Wen | 2011 | Biomaterials2011，，21： | 1 |
| 13 | Targeting the brain with PEG–PLGA nanoparticles modified with phage-displayed peptides显示文摘 | Jingwei Li Liang Feng Li Fan Yuan Zha Liangran Guo Qizhi Zhang Jun Chen Zhiqing Pang Yuchen Wang Xinguo Jiang Victor C. Yang Longping Wen | 2011 | Biomaterials2011，，21： | 1 |
| 14 | Targeting the brain with PEG–PLGA nanoparticles modified with phage-displayed peptides显示文摘 | Jingwei Li Liang Feng Li Fan Yuan Zha Liangran Guo Qizhi Zhang Jun Chen Zhiqing Pang Yuchen Wang Xinguo Jiang Victor C. Yang Longping Wen | 2011 | Biomaterials2011，，21： | 1 |
| 15 | Targeting the brain with PEG–PLGA nanoparticles modified with phage-displayed peptides显示文摘 | Jingwei Li Liang Feng Li Fan Yuan Zha Liangran Guo Qizhi Zhang Jun Chen Zhiqing Pang Yuchen Wang Xinguo Jiang Victor C. Yang Longping Wen | 2011 | Biomaterials2011，，21： | 1 |
| 16 | Down-regulation of TIPE2 mRNA expression in peripheral blood mononuclear cells from patients with systemic lupus erythematosus显示文摘 | Di Li Lijun Song Yuchen Fan Xia Li Yingjie Li Jie Chen Faliang Zhu Chun Guo Yongyu Shi Lining Zhang | 2009 | Clinical Immunology2009，，： | 1 |
| 17 | Ubiquitinspecific protease 34 regulates tooth root morphogenesis by stabilizing nuclear factor I/C显示文摘Tooth root morphogenesis involves two biological processes,root elongation and dentinogenesis,which are guaranteed by downgrowth of Hertwig’s epithelial root sheath(HERS)and normal odontoblast differentiation.Ubiquitin-dependent protein degradation has been reported to precisely regulate various physiological processes,while its role in tooth development is still elusive.Here we show ubiquitinspecific protease 34(USP34)plays a pivotal role in root formation.Deletion of Usp34 in dental mesenchymal cells leads to short root anomaly,characterized by truncated roots and thin root dentin.The USP34-deficient dental pulp cells(DPCs)exhibit decreased odontogenic differentiation with downregulation of nuclear factor I/C(NFIC).Overexpression of NFIC partially restores the impaired odontogenic potential of DPCs.These findings indicate that USP34-dependent deubiquitination is critical for root morphogenesis by stabilizing NFIC. | Shuang Jiang Rui Sheng Xingying Qi Jun Wang Yuchen Guo Quan Yuan | 2021 | International Journal of Oral Science2021，13，1： | 1 |
| 18 | TCP rebuilding for contentaware request dispatching in Web clusters 显示文摘 | Liu Yuchen Chiang Meiling Guo Lianfeng | 2007 | Software Practice & Experience2007，37，11： | 1 |
| 19 | Targeting the brain with PEG–PLGA nanoparticles modified with phage-displayed peptides显示文摘 | Jingwei Li Liang Feng Li Fan Yuan Zha Liangran Guo Qizhi Zhang Jun Chen Zhiqing Pang Yuchen Wang Xinguo Jiang Victor C. Yang Longping Wen | 2011 | Biomaterials2011，，21： | 1 |
| 20 | Targeting the brain with PEG–PLGA nanoparticles modified with phage-displayed peptides显示文摘 | Jingwei Li Liang Feng Li Fan Yuan Zha Liangran Guo Qizhi Zhang Jun Chen Zhiqing Pang Yuchen Wang Xinguo Jiang Victor C. Yang Longping Wen | 2011 | Biomaterials2011，，21： | 1 |
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