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| 1 | Targeted gene disruption by use of a group 11 intron (targetron) vector in Clostridium acetobutylicum显示文摘 | Lijun Shao Shiyuan Hu Yi Yang Yang Gu Jun Chen Yunliu Yang Weihong Jiang ShengYang | 2007 | Cell Research2007，17，11： | 23 |
| 2 | Natural Variation in CCD4 Promoter Underpins Species-Specific Evolution of Red Coloration in Citrus Peel显示文摘Carotenoids and apocarotenoids act as phytohormones and volatile precursors that influence plant development and confer aesthetic and nutritional value critical to consumer preference.Citrus fruits display considerable natural variation in carotenoid and apocarotenoid pigments.In this study,using an integrated genetic approach we revealed that a 5;c/s-regulatory change at CCD4b encoding CAROTENOID CLEAVAGE DIOXYGENASE 4b is a major genetic determinant of natural variation in C3 0 apocarotenoids responsible for red coloration of citrus peel.Functional analyses demonstrated that in addition the known role in synthesizing 3-citraurin,CCD4b is also responsible for the production of another important C3 0 apocarotenoid pigment,p-citraurinene.Furthermore,analyses of the CCD4b promoter and transcripts from various citrus germplasm accessions established a tight correlation between the presence of a putative 5'c/s-regulatory enhancer within an MITE transposon and the enhanced allelic expression of CCD4b in C3 0 apocarotenoid-rich red-peeled accessions.Phylogenetic analysis provided further evidence that functional diversification of CCD4b and naturally occurring variation of the CCD4b promoter resulted in the stepwise evolution of red peels in mandarins and their hybrids.Taken together,our findings provide new insights into the genetic and evolutionary basis of apocarotenoid diversity in plants,and would facilitate breeding efforts that aim to improve the nutritional and aesthetic value of citrus and perhaps other fruit crops. | Xiongjie Zheng Kaijie Zhu Quan Sun Weiyi Zhang Xia Wang Hongbo Cao Meilian Tan Zongzhou Xie Yunliu Zeng Junli Ye Lijun Chai Qiang Xu Zhiyong Pan Shunyuan Xiao Paul D.Fraser Xiuxin Deng | 2019 | Molecular Plant2019，12，9： | 18 |
| 3 | Gene cloning and function analysis of ABP9 protein which specifically binds to ABRE2 motif of maize Cat1 gene显示文摘A cDNA library was constructed using mRNA extracted from 17 days post-pollination (dpp) maize embryos and was screened by employing a yeast one-hybrid system for proteins specifically interacting with ABRE2 motif of maize Call gene. Three truncated overlapping positive clones designated ABP9 were obtained and the full-length cDNA was isolated by 5’ RACE. Searching the database revealed that ABP9 protein belongs to a bZIP-type transcription factor family. ABP9 protein speciflcally binds to ABRE2 motif and activates the expression of downstream reporter gene in yeast cells. Our results strongly suggest that the ABP9 protein functions as a transcription activator. | Lei Wang Jun Zhao Yunliu Fan | 2002 | Chinese Science Bulletin2002，47，22： | 14 |
| 4 | Integration of Metabolomics and Subcellular Organelle Expression Microarray to Increase Understanding the Organic Acid Changes in Post-harvest Citrus Fruit显示文摘Citric acid plays an important role in fresh fruit favor and its adaptability to post-harvest storage conditions.In order to explore organic acid regulatory mechanisms in post-harvest citrus fruit,systematic biological analyses were conducted on stored Hirado Buntan Pummelo(HBP;Citrus grandis)fruits.High-performance capillary electrophoresis,subcellular organelle expression microarray,real-time quantitative reverse transcription polymerase chain reaction,gas chromatography mass spectrometry(GC-MS),and conventional physiological and biochemical analyses were undertaken.The results showed that the concentration of organic acids in HBP underwent a regular fuctuation.GC-MS-based metabolic profling indicated that succinic acid,g-aminobutyric acid(GABA),and glutamine contents increased,but 2-oxoglutaric acid content declined,which further confrmed that the GABA shunt may have some regulatory roles in organic acid catabolism processes.In addition,the concentration of organic acids was signifcantly correlated with senescence-related physiological processes,such as hydrogen peroxide content as well as superoxide dismutase and peroxidase activities,which showed that organic acids could be regarded as important parameters for measuring citrus fruit post-harvest senescence processes. | Xiaohua Sun Andan Zhu Shuzhen Liu Ling Sheng Qiaoli Ma Li Zhang Elsayed Mohamed Elsayed Nishawy Yunliu Zeng Juan Xu Zhaocheng Ma Yunjiang Cheng Xiuxin Deng | 2013 | Journal of Integrative Plant Biology2013，55，11： | 14 |
| 5 | Integrated transcriptomic and metabolomic analyses of a wax deficient citrus mutant exhibiting jasmonic acid-mediated defense against fungal pathogens显示文摘Naturally,resistant crop germplasms are important resources for managing the issues of agricultural product safety and environment deterioration.We found a spontaneous mutant of‘Newhall’navel orange(Citrus sinensis Osbeck)(MT)with broad-spectrum protections against fungal pathogens in the orchard,postharvest-storage,and artificial inoculation conditions.To understand the defense mechanism of MT fruit,we constructed a genome-scale metabolic network that integrated metabolome and transcriptome datasets.The coordinated transcriptomic and metabolic data were enriched in two sub-networks,showing the decrease in very long chain fatty acid(by 41.53%)and cuticular wax synthesis(by 81.34%),and increase in the synthesis of jasmonic acid(JA)(by 95.23%)and JA-induced metabolites such as 5-dimethylnobietin(by 28.37%)in MT.Furthermore,cytological and biochemical analyses confirmed that the response to fungal infection in MT was independent of wax deficiency and was correlated with the levels of jasmonates,and the expression of plant defensin gene PDF1.2.Results of exogenous application of MeJA and JA inhibitors such as propyl gallate proved that JA-mediated defense contributes to the strong tolerance against pathogens in MT.Our results indicated that jasmonate biosynthesis and signaling are stimulated by the fatty acid redirection of MT,and participate in the tolerance of pathogenic fungi. | Yizhong He Jingwen Han Runsheng Liu Yuduan Ding Jinqiu Wang Li Sun Xiaoming Yang Yunliu Zeng Weiwei Wen Juan Xu Hongming Zhang Xiang Yan Zhaoxing Chen Zuliang Gu Hong Chen Huanqing Tang Xiuxin Deng Yunjiang Cheng | 2018 | Horticulture Research2018，5，1： | 8 |
| 6 | Isolation and Characterization of a C-repeat Binding Transcription Factor from Maize显示文摘C-repeat binding proteins (CBFs) are a group of transcription factors that have been proven to be important for stress tolerance in plants. Many of these transcription factors transactivate the promoters of cold-regulated genes via binding to low temperature-or dehydration-responsive cis-elements, thus conferring plants cold acclimation. In the present study, we isolated a C-repeat binding transcription factor from maize using the yeast one-hybrid system with the C-repeat motif from the promoter of the Arabidopsis COR15a gene as bait. The isolated transcription factor is highly similar to the Arabidopsis CBF3 in their predicted amino acid sequences, and is therefore designated ZmCBF3. Point mutation analyses of the ZmCBF3-binding cis-element revealed (A/G)(C/T)CGAC as the core binding sequence. Expression analyses showed that ZmCBF3 was upregulated by both abscisic acid and low temperature, and was actively expressed during embryogenesis, suggesting that ZmCBF3 plays a role in stress response in maize. | Lei Wang Yanzhong Luo Lan Zhang Jun Zhao Zhiqiu Hu Yunliu Fan Chunyi Zhang | 2008 | Journal of Integrative Plant Biology2008，50，8： | 6 |
| 7 | The Arabidopsis MutS homolog AtMSH5 is required for normal meiosis显示文摘MSH5,傻瓜相当或相同的事物脱氧核糖核酸失配修理蛋白质家庭的一个成员,被显示了在象老鼠,开始发育的酵母和 Caenorhabditis elegans 那样的多样的有机体为合适的同源染色体再结合被要求。在这篇论文,我们证明带通常认为的破坏 AtMSH5 基因的变异的 Arabidopsis 植物在 meiotic 期间展出缺点,生产不能生存的花粉粒和反常胚囊的一个比例,并且从而导致在富饶的减少。AtMSH5 表示被限制到 meiotic 花发芽,它在成熟分裂期间与一个可能的角色一致。男成熟分裂的 Cytological 分析从 diplotene 揭示了众多的 univalents 的存在到中期我,它在交叉频率与大减小被联系。在异种的剩余 chiasmata 的平均数字每性母细胞被归结为 2.54,它在野生型占约 25% 数量。这里,量的细胞遗传学的分析表明在 Atmsh5 异种的剩余 chiasmata 在 meiocytes 之中是随机分布式的,建议 AtMSH5 有在干扰敏感的交叉形成期间的一个必要角色。总起来说,证据显示 AtMSH5 通过在前期期间便于交叉形成支持相应再结合我在 Arabidopsis。 | Xiaoduo Lu Xiaolin Liu Lizhe An Wei Zhang Jian Sun Huijuan Pei Hongyan Meng Yunliu Fan Chunyi Zhang | 2008 | Cell Research2008，18，5： | 6 |
| 8 | Gene-lndexed Mutations in Maize显示文摘 | Xiaoduo Lu Jisheng Liu Wen Ren Qun Yang Zhenguang Chai Rumei Chen Lei Wang Jun Zhao Zhihong Lang Haiyang Wang Yunliu Fan Jiuran Zhao Chunyi Zhang | 2018 | Molecular Plant2018，11，3： | 4 |
| 9 | Evaluation of transgenic tobacco expressing two insecticidal genes to delay resistance development of Helicoverpa armigera显示文摘The resistance ratio of Helicoverpa armigera to Cry1Ac insecticidal protein from Bacillus thuringiensis (Bt) is 13.1- and 3.02-fold after 18 generations of selection by transgenic tobacco expressing Bt or two (Bt and CpTI) insecticidal protein genes, in which the average corrected mortality for each selection treatments is about 60% . The mortality of selected population by transgenic Bt gene tobacco is significantly lower than the control strain when fed | Jianzhou Zhao Yunliu Fan Xianlin Fan Xiping Shi Meiguang Lu | 1999 | Chinese Science Bulletin1999，44，20： | 3 |
| 10 | Isolation and functional analysis of transcription factor GmWRKY57B from soybean显示文摘WRKY proteins are a large family of transcriptional regulators in higher plants involved in many biological processes, such as responses to biotic and abiotic stress, plant development and metabolism. In this study, a soybean cDNA library was constructed and screened by yeast one-hybrid system using the 3×W-box element as a bait, and consequently a transcription factor, named GmWRKY57B, a member of group 3 of WRKY family, was obtained. GmWRKY57B specifically binds to the W-box of the Arabidopsis NPR1 (AtNPR1) promoter in yeast cells. This specific binding was further confirmed with an in vitro electrophoretic mobility shift assay. RT-PCR analysis showed that GmWRKY57B was expressed at a similar level in root, stem and leaf of the soybean plant. Overexpression of the GmWRKY57B gene in tobacco conferred the transgenics tolerance to drought stress. This result indicated that the GmWRKY57B gene plays a role in plant tolerance to abiotic stress. | ZHANG Lan WANG XlaoPing BI YingDong ZHANG ChunYi FAN YunLiu WANG Lei | 2008 | Chinese Science Bulletin2008，53，22： | 1 |
| 11 | Improving the thermostability of N-carbamyl-d-amino acid amidohydrolase by error-prone PCR显示文摘 | Hong Yu Jian Li Dalong Zhang Yunliu Yang Weihong Jiang Sheng Yang | 2009 | Applied Microbiology and Biotechnology2009，，2： | 1 |
| 12 | Risk factors for the prognosis of acute kidney injury under the Acute Kidney Injury Network definition: A retrospective, multicenter study in critically ill patients显示文摘 | JIAOJIAOZHOU LICHUANYANG KANGYIZHANG YUNLIU PINGFU | 2012 | Nephrology2012，，4： | 1 |
| 13 | Construction of a genomewide RNA i mutant library in rice显示文摘 | Lei Wang Jie Zheng Yanzhong Luo Tao Xu Qiuxue Zhang Lan Zhang Miaoyun Xu Jianmin Wan Ming‐Bo Wang Chunyi Zhang Yunliu Fan | 2013 | Plant Biotechnol J2013，，8： | 1 |
| 14 | Transgenic maize plants expressing a fungal phytase gene显示文摘 | Rumei Chen Guangxing Xue Ping Chen Bin Yao Wenzhu Yang Qianli Ma Yunliu Fan Zuoyu Zhao Mitchell C. Tarczynski Jinrui Shi | 2008 | Transgenic Research2008，，4： | 1 |
| 15 | Transgenic maize plants expressing a fungal phytase gene显示文摘 | Rumei Chen Guangxing Xue Ping Chen Bin Yao Wenzhu Yang Qianli Ma Yunliu Fan Zuoyu Zhao Mitchell C. Tarczynski Jinrui Shi | 2008 | Transgenic Research2008，，4： | 1 |
| 16 | A novel highly acidic β-mannanase from the acidophilic fungus Bispora sp. MEY-1: gene cloning and overexpression in Pichia pastoris显示文摘 | Huiying Luo Yaru Wang Hui Wang Jun Yang Yuhui Yang Huoqing Huang Peilong Yang Yingguo Bai Pengjun Shi Yunliu Fan Bin Yao | 2009 | Applied Microbiology and Biotechnology2009，，3： | 1 |
| 17 | Liquid chromatography tandem mass spectrometry method for determination of bisoprolol in human plasma using d5‐bisoprolol as the internal standard显示文摘 | Gang‐yiLiu WeiWang Jing‐yingJia ChuanLu Yan‐meiLiu Meng‐qiZhang YunLiu Shui‐junLi ChenYu | 2010 | Biomed Chromatogr2010，，6： | 1 |
| 18 | Gene cloning and expression of a new acidic family 7 endo-β-1,3-1,4-glucanase from the acidophilic fungus Bispora sp. MEY-1显示文摘 | Huiying Luo Jun Yang Peilong Yang Jiang Li Huoqing Huang Pengjun Shi Yingguo Bai Yaru Wang Yunliu Fan Bin Yao | 2010 | Applied Microbiology and Biotechnology2010，，4： | 1 |
| 19 | A comprehensive proteomic analysis of elaioplasts from citrus fruits reveals insights into elaioplast biogenesis and function显示文摘Elaioplasts of citrus peel are colorless plastids which accumulate significant amounts of terpenes.However,other functions of elaioplasts have not been fully characterized to date.Here,a LC–MS/MS shotgun technology was applied to identify the proteins from elaioplasts that were highly purified from young fruit peel of kumquat.A total of 655 putative plastid proteins were identified from elaioplasts according to sequence homology in silico and manual curation.Based on functional classification via Mapman,~50%of the identified proteins fall into six categories,including protein metabolism,transport,and lipid metabolism.Of note,elaioplasts contained ATP synthase and ADP,ATP carrier proteins at high abundance,indicating important roles for ATP generation and transport in elaioplast biogenesis.Additionally,a comparison of proteins between citrus chromoplast and elaioplast proteomes suggest a high level of functional conservation.However,some distinctive protein profiles were also observed in both types of plastids notably for isoprene biosynthesis in elaioplasts,and carotenoid metabolism in chromoplasts.In conclusion,this comprehensive proteomic study provides new insights into the major metabolic pathways and unique characteristics of elaioplasts and chromoplasts in citrus fruit. | Man Zhu Jiajia Lin Junli Ye Rui Wang Chao Yang Jinli Gong Yun Liu Chongling Deng Ping Liu Chuanwu Chen Yunjiang Cheng Xiuxin Deng Yunliu Zeng | 2018 | Horticulture Research2018，5，1： | 1 |
| 20 | NEW INSIGHTS INTO THE PHYLOGENY AND SPECIATION OF KUMQUAT (FORTUNELLA SPP.) BASED ON CHLOROPLAST SNP, NUCLEAR SSR AND WHOLE-GENOME SEQUENCING显示文摘Kumquat(Fortunella spp.)is a fruit and ornamental crop worldwide due to the palatable taste and high ornamental value of its fruit.Although Fortunella is classified into the economically important true citrus fruit tree group together with Citrus and Poncirus,few studies have been focused on its evolutionary scenario.In this study,analysis of five chloroplast loci and 47 nuclear microsatellites(nSSR)loci from 38 kumquat and 10 citrus accessions revealed the independent phylogeny of Fortunella among citrus taxa,and that Fortunella mainly comprises two populations:CUL,cultivated Fortunella spp.(F.margarita,F.crassifolia and F.japonica);and HK,wild Hong Kong kumquat(Fortunella hindsii).Genomic analysis based on whole-genome SNPs indicated that the allele frequency of both pupations deviated from the neutral selection model,suggesting directional selection was a force driving their evolutions.CUL exhibited lower genomic diversity and higher linkage strength than HK,suggesting artificial selection involved in its origin.A high level of genetic differentiation(Fst=0.364)was detected and obviously asynchronous demographic changes were observed between CUL and HK.Based on these results,a new hypothesis for the speciation of Fortunella is proposed. | Chenqiao ZHU Peng CHEN Junli YE Hang LI Yue HUANG Xiaoming YANG Chuanwu CHEN Chenglei ZHANG Yuantao XU Xiaoli WANG Xiang YAN Guangzhou DENG Xiaolin JIANG Nan WANG Hongxing WANG Quan SUN Yun LIU Di FENG Min YU Xietian SONG Zongzhou XIE Yunliu ZENG Lijun CHAI Qiang XU Chongling DENG Yunjiang CHENG Xiuxin DENG | 2022 | Frontiers of Agricultural Science and Engineering2022，9，4： | 0 |
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