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| 1 | Specific shRNA targeting of FAK influenced collagen metabolism in rat hepatic stellate cells显示文摘AIM:To investigate the effects and mechanism of disruption of focal adhesion kinase(FAK) expression on collagen metabolism in rat hepatic stellate cells(HSC).METHODS:The plasmids expressing FAK short hairpin RNA(shRNA) were transfected into HSC-T6 cells,and the level of FAK expression was determined by both real-time quantitative polymerase chain reaction(QPCR) and Western blotting analysis.The production of type collagen and type collagen in FAK-disrupted cells was analyzed by real-time Q-PCR.The level of collagen metabolism proteins,including matrix metalloproteinases-13(MMP-13) and tissue inhibitors of metalloproteinases-1(TIMP-1) was also determined by both real-time Q-PCR and Western blotting analysis.RESULTS:The transfection of FAK shRNA plasmids into HSC resulted in disrupted FAK expression.Compared with the HK group,the levels of type collagen and type collagen mRNA transcripts in FAK shRNA plas-mid group were signif icantly decreased(0.69 ± 0.03 vs 1.96 ± 0.15,P = 0.000;0.59 ± 0.07 vs 1.62 ± 0.12,P = 0.020).The production of TIMP-1 in this cell type was also signif icantly reduced at both mRNA and protein levels(0.49 ± 0.02 vs 1.72 ± 0.10,P = 0.005;0.76 ± 0.08 vs 2.31 ± 0.24,P = 0.000).However,the expression of MMP-13 mRNA could be significantly up-regulated by the transfection of FAK shRNA plasmids into HSC(1.74 ± 0.20 vs 1.09 ± 0.09,P = 0.000).CONCLUSION:These data support the hypothesis that shRNA-mediated disruption of FAK expression could attenuate extracellular matrix(ECM) synthesis and promote ECM degradation,making FAK a potential target for novel anti-f ibrosis therapies. | Zhi-Na Dun Xiao-Lan Zhang Jun-Yan An Li-Bo Zheng Robert Barrett Shu-Rui Xie | 2010 | World Journal of Gastroenterology2010，16，32： | 8 |
| 2 | Hyponatremia: evaluating the correction factor for hyperglycemia显示文摘 | Teresa A. Hillier Robert D. Abbott Eugene J. Barrett | 1999 | The American Journal of Medicine1999，，4： | 1 |
| 3 | A Multicenter Randomized Controlled Trial Comparing Patient-Controlled Epidural with Intravenous Analgesia for Pain Relief in Labor显示文摘 | Stephen H. Halpern Holly Muir Terrance W. Breen David C. Campbell Jon Barrett Robert Liston J Wade Blanchard | 2004 | Anesthesia & Analgesia2004，，5： | 1 |
| 4 | The relationship of presenting physical complaints to depressive symptoms in primary care patients显示文摘 | Paul D. Gerber James E. Barrett Jane A. Barrett Thomas E. Oxman Eric Manheimer Robert Smith Richard D. Whiting | 1992 | Journal of General Internal Medicine1992，，2： | 1 |
| 5 | In Vivo Molecular Imaging of Acute and Subacute Thrombosis Using a Fibrin-Binding Magnetic Resonance Imaging Contrast Agent显示文摘 | René M. Botnar Alexandra S. Perez Sonia Witte Andrea J. Wiethoff James Laredo James Hamilton William Quist Edward C. Parsons Anand Vaidya Andrew Kolodziej John A. Barrett Philip B. Graham Robert M. Weisskoff Warren J. Manning Michael T. Johnstone | 2004 | Circulation: Journal of the American Heart Association2004，，16： | 1 |
| 6 | Basic Epidemiology of Fractures of the Upper and Lower Limb among Americans over 65 Years of Age显示文摘 | John A. Baron Margaret Karagas Jane Barrett Wayne Kniffin David Malenka Michael Mayor Robert B. Keller | 1996 | Epidemiology1996，，6： | 1 |
| 7 | First human-caused extinction of a cetacean species?显示文摘 | Turvey Samuel T Pitman Robert L Taylor Barbara L Barlow Jay Akamatsu Tomonari Barrett Leigh A Zhao Xiujiang Reeves Randall R Stewart Brent S Wang Kexiong Wei Zhuo Zhang Xianfeng Pusser L.T Richlen Michael Brandon John R Wang Ding | 2007 | Biology Letters2007，，5： | 1 |
| 8 | Adverse selection and the decline in private health insurance coverage in Australia: 1989-95显示文摘 | Barrett GF Robert Conlon | 2003 | The Economic Record2003，79，246： | 1 |
| 9 | Org Lett 显示文摘 | Barrett A G M Cramp S M Robert R S | 2000 | 2(3): 261-2642000，2，3： | 1 |
| 10 | Market information and food insecurity response analysis显示文摘 | Christopher B Barrett Robert Bell Erin C Lentz | 2009 | Food Security2009，1，2： | 1 |
| 11 | The Potential of Myocardial Perfusion Scintigraphy for Risk Stratification of Asymptomatic Patients With Type 2 Diabetes显示文摘 | Jeroen J. Bax Robert O. Bonow Diethelm Tsch?pe Silvio E. Inzucchi Eugene Barrett | 2006 | Journal of the American College of Cardiology2006，，4： | 1 |
| 12 | Corporate Philanthropy,Criminal Activity,and Firm Reputation:Is There a Link? 显示文摘 | ROBERT J W BARRETT J D | 2000 | Journal of Business Ethics2000，26，4： | 1 |
| 13 | Frameless stereotactic aspiration and thrombolysis of spontaneous intracerebral hemorrhage显示文摘 | Ryan J. Barrett Rahat Hussain William M. Coplin Samera Berry Penelope M. Keyl Daniel F. Hanley Robert R. Johnson J. Ricardo Carhuapoma | 2005 | Neurocritical Care2005，，3： | 1 |
| 14 | Three-dimensional reconstruction from planar projections显示文摘 | Ming-Yee Chiu Harrison H Barrett Robert G Simpson | 1980 | J Opt Soc Am1980，70，7： | 1 |
| 15 | Corporate Philanthropy, Criminal Activity, and Firm Reputation: Is There a Link?显示文摘 | Robert J. Williams J. Douglas Barrett | 2000 | Journal of Business Ethics2000，，4： | 1 |
| 16 | Randomized trial of lamivudine versus hepatitis B immunoglobulin for long-term prophylaxis of hepatitis B recurrence after liver transplantation显示文摘 | Nikolai V Naoumov A.Ross Lopes Patrizia Burra Lucio Caccamo Rosa M Iemmolo Robert A de Man Margaret Bassendine John G O’Grady Bernard C Portmann Gaya Anschuetz Catherine A Barrett Roger Williams Mark Atkins | 2001 | Journal of Hepatology2001，，6： | 1 |
| 17 | The Synthesis and Structure o{ Lithium Derivatives of the Sterieally Encumbered β-diketiminate Ligand ^-, and a Modified Synthesis of the Aminoimine Precursor显示文摘 | StENDER MATTHIAS WRIGHT ROBERT J EICHLER BARRETT E | 2001 | J Chem Soc Dalton Trans2001，，： | 1 |
| 18 | A High-Fiber Diet Does Not Protect Against Asymptomatic Diverticulosis显示文摘 | Anne F. Peery Patrick R. Barrett Doyun Park Albert J. Rogers Joseph A. Galanko Christopher F. Martin Robert S. Sandler | 2012 | Gastroenterology2012，，2： | 1 |
| 19 | Constitutive TL1A Expression under Colitogenic Conditions Modulates the Severity and Location of Gut Mucosal Inflammation and Induces Fibrostenosis显示文摘 | Robert Barrett Xiaolan Zhang Hon Wai Koon Michelle Vu Jyh-Yau Chang Nicole Yeager Mary Ann Nguyen Kathrin S. Michelsen Dror Berel Charalabos Pothoulakis Stephan R. Targan David Q. Shih | 2012 | The American Journal of Pathology2012，，2： | 1 |
| 20 | Azole Resistance in Candida albicans显示文摘 | John F Ryley Robert G Wilson Keith J Barrett - Bee | 1984 | Medical Mycology1984，22，1： | 1 |
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