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| 1 | Effects of Plant Growth-Promoting Rhizobacteria and N Source on Plant Growth and N and P Uptake by Tomato Grown on Calcareous Soils显示文摘Introducing specific microorganisms into the soil ecological system is an important strategy for improving nutrient use efficiency.Two pot experiments were conducted in the greenhouse from December 3, 2012 to January 25, 2013(Experiment 1) and March 11 to April 23, 2013(Experiment 2) to evaluate the effect of nitrogen(N) source and inoculation with plant growth-promoting rhizobacteria(PGPR) on plant growth and N and phosphorus(P) uptake in tomato(Lycopersicon esculentum Mill.) grown on calcareous soils from South Florida, USA.Treatments included urea, controlled release urea(a controlled release fertilizer, CRF) each at low and high N rates and with or without inoculation of PGPR.A mixture of PGPR strains Bacillus amyloliquefaciens IN937 a and Bacillus pumilus T4 was applied to the soil during growing periods of tomato.Treatments with PGPR inoculation increased plant height compared to treatments without PGPR in both experiments.Inoculation with PGPR increased shoot dry weight and shoot N uptake for the same N rate and N source.In both experiments, only at high N rate, CRF and urea treatments with PGPR had significantly(P < 0.05)greater shoot biomass than those without PGPR.Only at high N rate, CRF treatment with PGPR significantly increased shoot N uptake by 39.0% and 10.3% compared to that without PGPR in Experiments 1 and 2, respectively.Meanwhile, presence of PGPR in the soil increased shoot P uptake for all treatments in Experiment 1 and for most treatments in Experiment 2.In Experiment 1, only at low N rate, CRF treatment with PGPR significantly increased shoot P uptake compared with that without PGPR.In Experiment2, a significant increase in shoot P uptake by inoculation of PGPR was only observed in CRF treatment at high N rate.Results from this study indicate that inoculation with PGPR may increase plant growth and N and P uptake by tomato grown on calcareous soils.However, the effect of PGPR varied and was influenced by many factors such as N source, N rate, and soil fertility.Further investigations are warranted to confirm the effect of PGPR under different soil conditions. | Xiaohui FAN Shouan ZHANG Xiaodan MO Yuncong LI Yuqing FU Zhiguang LIU | 2017 | Pedosphere2017，27，6： | 8 |
| 2 | MdMYB8 is associated with flavonol biosynthesis via the activation of the MdFLS promoter in the fruits of Malus crabapple显示文摘Flavonols are polyphenolic compounds that play important roles in plant stress resistance and development.They are also valuable components of the human diet.The Malus crabapple cultivar‘Flame’provides an excellent model for studying flavonol biosynthesis due to the high flavonol content of its fruit peel.To obtain a more detailed understanding of the flavonol regulatory network involved in fruit development,the transcriptomes of the fruit of the Malus cv.‘Flame’from five continuous developmental stages were analyzed using RNA sequencing.A flavonol-related gene module was identified through weighted gene coexpression network analysis(WGCNA),and Kyoto Encyclopedia of Genes and Genomes(KEGG)analysis indicated that phytohormones are involved in regulating flavonol biosynthesis during fruit development.A putative transcription factor,MdMYB8,was selected for further study through hub gene correlation network analysis and yeast one-hybrid assays.Stable overexpression or RNAi knockdown of MdMYB8 in transgenic‘Orin’apple calli resulted in a higher or lower flavonol content,respectively,suggesting that MdMYB8 is a regulator of flavonol biosynthesis.This transcriptome analysis provides valuable data for future studies of flavonol synthesis and regulation. | Hua Li Yu Li Jiaxuan Yu Ting Wu Jie Zhang Ji Tian Yuncong Yao | 2020 | Horticulture Research2020，7，1： | 6 |
| 3 | BODIPY-derived ratiometric fluorescent sensors: pH-regulated aggregation-induced emission and imaging application in cellular acidification triggered by crystalline silica exposure显示文摘Modification of classic fluorophore to possess the emission transitions between aggregation-induced emission(AIE)and intrinsic emission offers reliable approach to the design of ratiometric fluorescent sensors.In this study,a proton acceptor benzimidazole was integrated with BODIPY to form three compounds,BBI-1/2/3,which demonstrated the AIE(~595 nm,I_(agg))in neutral aqueous medium and intrinsic BODIPYemission(~510 nm,I_(int))in acidic medium.All the three showed the ratiometric pH sensing behavior in a dual excitation/dual emission mode,yet BBI-3 displayed still the dual emission ratiometric pH sensing ability.The pH-dependent emission ratio I_(int)/I_(agg)of the three were fully reversible,and no interference was observed from normal abundant chemical species in live cells.Their different p K_a(BBI-1,p K_a4.4;BBI-2,p K_a2.7;BBI-3,p K_a3.6)suggested that the substituents on benzimidazole moiety were essential to govern their functioning pH range.The ratiometric imaging of BBI-1 in A549 cells provided an effective intracellular pH(pH_i)calibration formula corresponding to emission ratio of I_(int)/I_(agg).Ratiometric pH_iimaging in A549 cells upon small particle exposure confirmed the particle-induced cellular acidification with this formula.Both particle size and the chemical nature of the particle contribute to the observed acidification effect.The synchronization of lysosome disruption to cellular acidification in A549 cells upon crystalline silica exposure was directly observed for the first time with BBI-1,showing the potential application of BBI-1 in the study of silicosis and other related diseases.This study demonstrated that endowing fluorophore with AIE/intrinsic emission transition could be a promising strategy for ratiometric sensor design. | Yang Bai Danyang Liu Zhong Han Yuncong Chen Zhongyan Chen Yang Jiao Weijiang He Zijian Guo | 2018 | Science China Chemistry2018，61，11： | 4 |
| 4 | Comparative Transcriptome Analysis Reveals Different Host Cell Responses to Acute and Persistent Foot-and-Mouth Disease Virus Infection显示文摘Foot-and-mouth disease virus(FMDV) rapidly causes cytopathic effects in susceptible cells. Incomplete viral clearance during the acute infection leads to persistent infection. The relationship between host gene expression and the persistent infection remains unclear. In this study, we analyzed the transcriptome profiles of BHK-21 cells acutely and persistently infected with FMDV to identify differences in gene expression. GO and KEGG enrichment analyses showed that the 8,378 differentially expressed genes were significantly enriched in categories including metabolism, biosynthesis, ribosome function, and endocytosis. In persistently infected BHK-21 cells, ribosome-and translation-related genes were significantly down-regulated. There were more differentially expressed immune-related genes during persistent infection than during acute infection. Two hundred and seventy-four genes were differentially expressed in both acutely and persistently infected BHK-21 cells. Among these genes, heat shock protein family B member 1(Hspb1) knockdown significantly inhibited FMDV replication. Our research provides a basis for further research to understand the mechanisms of persistent FMDV infection including the genes involved in FMDV replication. | Jiadai Li Lingling Han Yao Hao Yuncong Yuan Mingzhen Wang Xiu Xin Hailong Wang Fang Yu Congyi Zheng Chao Shen | 2020 | Virologica Sinica2020，35，1： | 4 |
| 5 | Determination of Fe,Ni,Cu,Zn,Cd and Pb in seawater by isotope dilution automatic solid-phase extraction—ICP-MS显示文摘A thorough understanding of the biogeochemical cycling of trace metals in the ocean is crucial because of the important role these elements play in regulating metabolism in marine biotas and thus,the climate.However,a precise and accurate analysis of trace metals in seawater is difficult because they are present at extremely low concentrations in a high salt matrix.In this study,we report an analytical method for the preconcentration and separation of six trace metals,Fe,Ni,Cu,Zn,Cd and Pb,in seawater using a sea FAST automatic solid-phase extraction device,analysis by a triple quadrupole collision/reaction technique with inductively coupled plasma mass spectrometry(ICP-MS),and quantification by the isotope dilution technique.A small volume(10 m L)of seawater sample was mixed with a multi-element isotope spike and subjected to sea FAST procedures.The preconcentrate solution was then analyzed using the optimized collision/reaction cell mode of ICP-MS,with NH_(3)gas for Fe and Cd with a flow rate of 0.22 m L/min and He for Ni,Cu,Zn and Pb with a flow rate of 4.0 m L/min.The procedure blanks were 130 pmol/L,3.0 pmol/L,6.8 pmol/L,37 pmol/L,0.29 pmol/L and 0.42 pmol/L,for Fe,Ni,Cu,Zn,Cd and Pb,respectively.The method was validated using five reference materials(SLRs-6,SLEW-3,CASS-6,NASS-7 and GEOTRACE-GSC),and our results were consistent with the consensus values.The method was further validated by measuring full-water-column seawater samples from the subtropical Northwest Pacific Ocean,and our results demonstrated good oceanic consistency. | Yuncong Ge Ruifeng Zhang Ziyuan Jiang Zhan Shen Maojun Yan | 2022 | Acta Oceanologica Sinica2022，41，8： | 3 |
| 6 | Identification, through transcriptome analysis, of transcription factors that regulate anthocyanin biosynthesis in different parts of red-fleshed apple 'May' fruit显示文摘Anthocyanins, a class of flavonoid compounds that provide pigmentation to plant organs, are beneficial components of the human diet and there is an interest in understanding the regulation of their formation in crop plants. The apple cultivar ‘May’ represents an excellent model for the study of anthocyanin metabolism due to the spatial color pattern of its flesh, which transitions sequentially from red to white to red again,from the outside to the inside of the apple fruit. To screen the transcription factors involved in the anthocyanin pathway, transcriptomes from differently colored sections of ‘May’ fruits were analyzed by RNA sequencing. Kyoto Encyclopedia of Genes and Genomes(KEGG) database was used to analyse the gene expression data. The results suggested differentially expressed genes(DEGs) that are related to phytohormones are involved in regulating anthocyanin biosynthesis. K-means clustering analysis revealed 167 common DEGs between different sections of fruit with the same expression pattern as candidates for regulating anthocyanin synthesis. Further analysis showed that nine of the 167 DEGs were annotated as transcription factors and quantitative real time PCR(qRT-PCR) confirmed that their expression was obviously higher in red regions of the fruit, consistent with their roles as hub genes that regulate anthocyanin synthesis. This study provides valuable results for future studies of anthocyanin synthesis in apple fruit. | Hua Li Shiyao Duan Weilei Sun Sifan Wang Jie Zhang Tingting Song Ji Tian Yuncong Yao | 2022 | Horticultural Plant Journal2022，8，1： | 2 |
| 7 | Visual Attention Model Based Regions of Interest Detection in Compressed Domain显示文摘 | SUI Lei ZHANG Jing ZHUO Li YANG Yuncong | 2012 | Chinese Journal of Electronics2012，21，4： | 2 |
| 8 | Removal of arsenic by magnetic biochar prepared from pinewood and natural hematite显示文摘 | Shengsen Wang Bin Gao Andrew R Zimmerman Yuncong Li Lena Ma Willie Harris Kati Migliaccio | 2014 | Bioresource Technology2014，，： | 2 |
| 9 | Epigenetic regulation in cardiovascular disease:mechanisms and advances in clinical trials显示文摘Epigenetics is closely related to cardiovascular diseases.Genome-wide linkage and association analyses and candidate gene approaches illustrate the multigenic complexity of cardiovascular disease.Several epigenetic mechanisms,such as DNA methylation,histone modification,and noncoding RNA,which are of importance for cardiovascular disease development and regression.Targeting epigenetic key enzymes,especially the DNA methyltransferases,histone methyltransferases,histone acetylases,histone deacetylases and their regulated target genes,could represent an attractive new route for the diagnosis and treatment of cardiovascular diseases.Herein,we summarize the knowledge on epigenetic history and essential regulatory mechanisms in cardiovascular diseases.Furthermore,we discuss the preclinical studies and drugs that are targeted these epigenetic key enzymes for cardiovascular diseases therapy.Finally,we conclude the clinical trials that are going to target some of these processes. | Yuncong Shi Huanji Zhang Suli Huang Li Yin Feng Wang Pei Luo Hui Huang | 2022 | Signal Transduction and Targeted Therapy2022，7，7： | 2 |
| 10 | Synthetic poly-dioxolane as universal solid electrolyte interphase for stable lithium metal anodes显示文摘Lithium (Li) metal is a promising anode for the next generation high-energy–density batteries. However, the growth of Li dendrites, low coulombic efficiency and dramatic volume change limit its development. Here, we report a new synthetic poly-dioxolane (PDOL) approach to constructing an artificial 'elastic' SEI to stabilize the Li/electrolyte interface and the Li deposition/dissolution behavior in a variety of electrolytes. By coating PDOL with optimized molecular weights and synthetic routes on Li metal anode, the 'elastic' SEI layer could be maintained on top of the Li metal anode to accommodate the Li deposition/dissolution. No dendrite formation was observed during the cycling process, and the interfacial side reactions were reduced significantly. Consequently, we successfully achieved 330 cycles with a CE of 98.4% in ether electrolytes and 90 cycles with a CE of 94.3% in carbonate electrolytes. Simultaneously, the Li-metal batteries with LiFePO_(4) as cathodes also exhibited improved cycling performance. This strategy could promote the development of dendrite-free metal anodes toward high-performance Li-metal batteries. | Tao Chen Haiping Wu Jing Wan Mengxue Li Yucheng Zhanga Lin Sun Yuncong Liu Lili Chen Rui Wen Chao Wang | 2021 | Journal of Energy Chemistry2021，30，11： | 1 |
| 11 | Coal fly ash as an amendment to container substrate for Spathiphyllum production显示文摘 | Jianjun Chen Yuncong Li | 2005 | Bioresource Technology2005，，15： | 1 |
| 12 | Isolation and expression of McF3H gene in the leaves of crabapple显示文摘 | Hongxiang Shen Jie Zhang Yuncong Yao Ji Tian Tingting Song Jian Geng Junping Gao | 2012 | Acta Physiologiae Plantarum2012，，4： | 1 |
| 13 | Effects of porous clay ceramic rates on aeration porosity characteristics in a structurally degraded soil under greenhouse vegetable production显示文摘Soil structure degradation in greenhouse vegetable fields reduces vegetable production. Increasing aeration porosity is the key to ameliorating soil structure degradation. Thus, we tested the effect of a porous material, porous clay ceramic(PLC), on the amelioration of soil structure degradation under greenhouse vegetable production. A 6-month pot experiment was conducted with four PLC application levels based on volume, i.e., 0%(control), 5%(1 P), 10%(2 P), and15%(3 P) using Brassica chinensis as the test plant. At the end of the experiment, soil columns were sampled, and the aeration pore network was reconstructed using X-ray computed tomography(CT). The degree of anisotropy(DA), fractal dimension(FD), connectivity, aeration porosity, pores distribution, and shape of soil aeration pores and plant biomass were determined. The DA, FD, and connectivity did not significantly differ as the PLC application rate increased.Nonetheless, aeration porosity significantly linearly increased. The efficiency of PLC at enhancing soil aeration porosity was 0.18% per Mg ha^(-1). The increase in aeration porosity was mainly due to the increase in pores > 2 000 μm, which was characterized by irregular pores. Changes in aeration porosity enhanced the production of B. chinensis. The efficiency of PLC at increasing the plant fresh weight was 0.60%, 3.06%, and 2.12% per 1% application rate of PLC for the 1 P, 2 P, and 3 P treatments, respectively. These results indicated that PLC is a highly efficient soil amendment that improves soil structure degradation by improving soil aeration under greenhouse conditions. Based on vegetable biomass, a 10% application rate of PLC was recommended. | Quanbo YU Meiyan WANG Yutian TIAN Xuezheng SHI Xiangwei LI Lingying XU Xinqiao XIE Yijie SHI Yuncong ZHU | 2021 | Pedosphere2021，31，4： | 1 |
| 14 | Aromatic plants play an important role in promoting soil biologicalactivity related to nitrogen cycling in an orchard ecosystem显示文摘 | Chen Xinxin Song Beizhou Yao Yuncong | 2014 | Science of the Total Environment2014，，472： | 1 |
| 15 | Response of containergrown apple trees to soil compaction显示文摘 | FERREE D C STREETER J G YUNCONG Y | 2004 | Hortscience2004，39，： | 1 |
| 16 | Land usedisturbance indicators and water quality variability in the BiscayneBay Watershed,Florida显示文摘 | CAREY R O MIGLIACCIO K W LI Yuncong | 2011 | Ecological Indicators2011，11，： | 1 |
| 17 | Genotypic differences in potassium nutrition in lowland ricehybrids显示文摘 | Guodong Liu Yuncong Li David Marshall Porterfield | 2009 | Communications in Soil Science and Plant Analysis2009，40，1112： | 1 |
| 18 | Engineered carbon (biochar) prepared by direct pyrolysis of Mg-accumulated tomato tissues: Characterization and phosphate removal potential显示文摘 | Ying Yao Bin Gao Jianjun Chen Ming Zhang Mandu Inyang Yuncong Li Ashok Alva Liuyan Yang | 2013 | Bioresource Technology2013，，： | 1 |
| 19 | Response of container-grown apple trees to soil compaction显示文摘 | Ferree D C Streeter J G Yuncong Y | 2004 | Hor Sci2004，39，1： | 1 |
| 20 | Global pattern of carbon stable isotopes of suspended particulate organic matter in lakes显示文摘 | DONGYAN L GANG CH YUNCONG L | 2012 | Limnology2012，13，： | 1 |
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