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33篇 您的检索式:作者名="Ruichao LI"
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1Numerical simulation of particle flow behavior in a screw conveyor using the discrete element method显示文摘Particle motion in a screw conveyor was simulated w让h the discrete element method. The particle flow behavior and transport processes at different screw rotating speeds and filling levels were investigated in this study. The spatial distributions of particle velocity were predicted. The predicted mass flow rate increased w让h increasing screw rotating speed and filling level. The contact forces and granular temperatures of particles were also calculated. The simulation results showed that the translational granular particle temperatures were higher than the rotational granular particle temperatures. In add让ion, the configurational temperatures of particles were calculated from simulated instantaneous particle overlaps, and results indicated that deformation of elastic particles contributed to the rate of energy dissipation. Good agreement between the numerical simulation and experimental results was achieved in this study.Shuyan Wang Haolong Li Ruichao Tian Ruichen Wang Xu Wang Qiji Sun jiawei Fan 2019Particuology2019,17,2:7
2Clinical management and survival outcomes of patients withdifferent molecular subtypes of diffuse gliomas in China(2011–2017):a multicenter retrospective study from CGGA显示文摘Objective:We aimed to summarize the clinicopathological characteristics and prognostic features of various molecular subtypes of diffuse gliomas(DGs)in the Chinese population.Methods:In total,1,418 patients diagnosed with DG between 2011 and 2017 were classified into 5 molecular subtypes according to the 2016 WHO classification of central nervous system tumors.The IDH mutation status was determined by immunohistochemistry and/or DNA sequencing,and 1p/19q codeletion was detected with fluorescence in situ hybridization.The median clinical follow-up time was 1,076 days.T-tests and chi-square tests were used to compare clinicopathological characteristics.Kaplan‒Meier and Cox regression methods were used to evaluate prognostic factors.Results:Our cohort included 15.5%lower-grade gliomas,IDH-mutant and 1p/19q-codeleted(LGG-IDHm-1p/19q);18.1%lowergrade gliomas,IDH-mutant(LGG-IDHm);13.1%lower-grade gliomas,IDH-wildtype(LGG-IDHwt);36.1%glioblastoma,IDHwildtype(GBM-IDHwt);and 17.2%glioblastoma,IDH-mutant(GBM-IDHm).Approximately 63.3%of the enrolled primary gliomas,and the median overall survival times for LGG-IDHm,LGG-IDHwt,GBM-IDHwt,and GBM-IDHm subtypes were 75.97,34.47,11.57,and 15.17 months,respectively.The 5-year survival rate of LGG-IDHm-1p/19q was 76.54%.We observed a significant association between high resection rate and favorable survival outcomes across all subtypes of primary tumors.We also observed a significant role of chemotherapy in prolonging overall survival for GBM-IDHwt and GBM-IDHm,and in prolonging post-relapse survival for the 2 recurrent GBM subtypes.Conclusions:By controlling for molecular subtypes,we found that resection rate and chemotherapy were 2 prognostic factors associated with survival outcomes in a Chinese cohort with DG.Kenan Zhang Xing Liu Guanzhang Li Xin Chang Shouwei Li Jing Chen Zheng Zhao Jiguang Wang Tao Jiang Ruichao Chai 2022Cancer Biology & Medicine2022,19,10:4
3Progress in the diagnosis and treatment of extensive-stage small cell lung cancer显示文摘Lung cancer, being the most common cancer type, accounts for 13% of all newly diagnosed malignant tumors globally each year. Small cell lung cancer(SCLC) accounts for approximately 15% of newly diagnosed lung cancers each year, but its annual death toll accounts for 25% of that of lung cancer. We summarized relevant clinical studies to elaborate the epidemiology, pathological and clinical characteristics and the treatment status of small cell lung cancer. This paper first described the epidemiology and the pathological and clinical characteristics of SCLC and the systematic treatment of extensive-stage SCLC and then introduced the current targeted therapy and immunotherapy for SCLC to provide clinicians and patients with a more systematic, comprehensive, and beneficial treatment regimen. We expect that these studies can provide clinicians with a clear direction in molecularly targeted therapy or immunotherapy, so that a treatment approach with better antitumor effects and longer-lasting clinical benefits can be provided to the patients.Fei Xu Xiaoli Ren Yuan Chen Qianxia Li Ruichao Li Yu Chen Shu Xia 2019Oncology and Translational Medicine2019,5,1:3
4Exhausted local lactate accumulation via injectable nanozyme-functionalized hydrogel microsphere for inflammation relief and tissue regeneration显示文摘Local lactate accumulation greatly hinders tissue repair and regeneration under ischemic condition.Herein,an injectable microsphere(MS@MCL)for local lactate exhaustion was constructed by grafting manganese dioxide(MnO_(2))-lactate oxidase(LOX)composite nanozyme on microfluidic hyaluronic acid methacrylate(HAMA)microspheres via chemical bonds,achieving a long-term oxygen-promoted lactate exhaustion effect and a long half-life in vivo.The uniform and porous microspheres synthesized by microfluidic technology is beneficial to in situ injection therapy and improving encapsulation efficiency.Furthermore,chemical grafting into HAMA microspheres through amide reactions promoted local enzymatic concentration and activity enhancement.It was showed that the MS@MCL eliminated oxidative and inflammatory stress and promoted extracellular matrix metabolism and cell survival when co-cultured with nucleus pulposus cells(NPCs)in vitro.In the rat degenerative intervertebral disc model caused by lactate injection,MS@MCL showed a long-term therapeutic effect in reducing intervertebral height narrowing and preventing extracellular matrix(ECM)degradation as well as inflammatory damage in vivo.Altogether,this study confirms that this nanozyme-functionalized injectable MS@MCL effectively improves the regenerative and reparative effect in ischemic tissues by disposing of enriched lactate in local microenvironment.Jieliang Shen Ao Chen Zhengwei Cai Zhijie Chen Ruichao Cao Zongchao Liu Yuling Li Jie Hao 2022Bioactive Materials2022,7,6:2
5Predictive value of MGMT promoter methylation on the survival of TMZ treated IDH-mutant glioblastoma显示文摘Objective:O6 methylguanine-DNA methyltransferase(MGMT)promoter methylation is a biomarker widely used to predict the sensitivity of IDH-wildtype glioblastoma to temozolomide therapy.Given that the IDH status has critical effects on the survival and epigenetic features of glioblastoma,we aimed to assess the role of MGMT promoter methylation in IDH-mutant glioblastoma.Methods:This study included 187 IDH-mutant glioblastomas and used 173 IDH-wildtype glioblastomas for comparison.KaplanMeier curves and multivariate Cox regression were used to study the predictive effects.Results:Compared with IDH-wildtype glioblastomas,IDH-mutant glioblastomas showed significantly higher(P<0.0001)MGMT promoter methylation.We demonstrated that MGMT promoter methylation status,as determined by a high cutoff value(≥30%)in pyrosequencing,could be used to significantly stratify the survival of 50 IDH-mutant glioblastomas receiving temozolomide therapy(cohort A);this result was validated in another cohort of 25 IDH-mutant glioblastomas(cohort B).The median progression-free survival and median overall survival in cohort A were 9.33 and 13.76 months for unmethylated cases,and 18.37 and 41.61 months for methylated cases,and in cohort B were 6.97 and 9.10 months for unmethylated cases,and 23.40 and 26.40 months for methylated cases.In addition,we confirmed that the MGMT promoter methylation was significantly(P=0.0001)correlated with longer OS in IDH-mutant patients with GBM,independently of age,gender distribution,tumor type(primary or recurrent/secondary),and the extent of resection.Conclusions:MGMT promoter methylation has predictive value in IDH-mutant glioblastoma,but its cutoff value should be higher than that for IDH-wildtype glioblastoma.Ruichao Chai Guanzhang Li Yuqing Liu Kenan Zhang Zheng Zhao Fan Wu Yuzhou Chang Bo Pang Jingjun Li Yangfang Li Tao Jiang Yongzhi Wang 2021Cancer Biology & Medicine2021,18,1:2
6Genomic Epidemiology of ST34 Monophasic Salmonella enterica Serovar Typhimurium from Clinical Patients from 2008 to 2017 in Henan,China显示文摘Salmonella enterica serovar 4,[5],12:i:-(S.4,[5],12:i:-)is a monophasic variant of Salmonella enterica serovar Typhimurium that has emerged as a global serovar causing public health concern.To date,the epidemiology and genomic characterization of this pathogen in China have not been well described.We investigated the prevalence,antimicrobial resistance(AMR)phenotypes,and population genomics of sequence type 34(ST34)S.4,[5],12:i:-among cases of human salmonellosis in Henan Province,China.A total of 100 ST34 S.4,[5],12:i:-isolates were studied from 2008 to 2017 and found mostly resistant to ampicillin(AMP),streptomycin(STR),sulfonamides(SUL),and tetracycline(TET)(ASSu T).Bayesian phylogenetic analysis demonstrated that isolates identified in China were mostly related to the European lineage and evolved into two major clades with different resistance genes and plasmid profiles.Notably,clade 1 showed a significantly higher rate of mutations in gyr A and plasmid-mediated quinolone resistance genes.The carrying of the resistance-containing region(encoding R-type ASSu T),including bla(conferring resistance to AMP),str AB(STR),sul2(SUL),and tet(B)(TET)inserted into the flj BA operon,was responsible for most of the monophasic variants in clade 2.Inc HI2 plasmids were the dominant multi-drug resistance mobile genetic elements accounting for the transmission of acquired resistance genes in this serovar,and these were more prevalent in clade 1.Our findings highlighted the increasing prevalence of multi-drug resistant S.4,[5],12:i:-in China,along with the differential characteristics of resistance gene acquisition among various lineages.Based on our data,control measures are required to address the spread of this zoonotic pathogen.Further owing to its potential origin in food-producing animals,a'One Health'approach,should be implemented to support surveillance whilst informing interventional strategies.Yujiao Mu Ruichao Li Pengcheng Du Pei Zhang Yan Li Shenghui Cui Séamus Fanning Li Bai 2022Engineering2022,8,8:2
7Takagi-Sugeno fuzzy model identification for turbofan aero-engines with guaranteed stability显示文摘This paper is concerned with identifying a Takagi-Sugeno(TS) fuzzy model for turbofan aero-engines working under the maximum power status(non-afterburning). To establish the fuzzy system, theoretical contributions are made as follows. First, by fixing antecedent parameters, the estimation of consequent parameters in state-space representations is formulated as minimizing a quadratic cost function. Second, to avoid obtaining unstable identified models, a new theorem is proposed to transform the prior-knowledge of stability into constraints. Then based on the aforementioned work, the identification problem is synthesized as a constrained quadratic optimization.By solving the constrained optimization, a TS fuzzy system is identified with guaranteed stability.Finally, the proposed method is applied to the turbofan aero-engine using simulation data generated from an aerothermodynamics component-level model. Results show the identified fuzzy model achieves a high fitting accuracy while stabilities of the overall fuzzy system and all its local models are also guaranteed.Ruichao LI Yingqing GUO Sing Kiong NGUANG Yifeng CHEN 2018Chinese Journal of Aeronautics2018,31,6:2
8China’s new problems of food security revealed by the Food Equivalent Unit显示文摘China's food security has a great influence on the world,and has always been the top priority in China.In recent years,as the concept of food security is evolving into one of nutrition security and the importance of food diversity is increasing,research based on nutrition security and broad food systems are increasingly needed in today’s China.Thus,not only grain for human consumption,but also animal foods have been integrated into the Food Equivalent,which is used to analyze China’s current agriculture system and reveal the water resource distribution.The results indicated that the average animal food consumption has risen by 78.6%,and now China’s daily supply of animal food per capita has reached about 50%of that in the USA and 80%of that in the South Korea.So there exist an obvious disparity in animal food supply between China and these two countries.It is impossible for the China’s current agricultural system to achieve the level in the USA.Under China’s current agricultural system,the consumption proportion of feed grain had surpassed the consumption of food grain,increased sharply from 33%in 1992 to 67%in 2011.However,the growth potential of total grain output is approaching an upper limit,so the continued growth of feed grain demand exerts great pressure on the China’s food supply.The discordance of the spatial distribution of water resource and virtual water revealed that China’s current agriculture system had a low efficiency in being able to achieve food and nutrition security.China’s current“grain farming”cannot meet the demand of increasing nutrition and appropriate resource utilization.The implementation of grassland agriculture appears feasible and necessary for saving feed grain,providing a large number of high-quality animal foods and appropriate water resource utilization.Huilong LIN Ruichao LI Cangyu JIN Chong WANG Maohong WEI Jizhou REN 2014Frontiers of Agricultural Science and Engineering2014,1,1:2
9The interaction between polyphyllin I and SQLE protein induces hepatotoxicity through SREBP-2/HMGCR/SQLE/LSS pathway显示文摘Polyphyllin I(PPI)and polyphyllin II(PII)are the main active substances in the Paris polyphylla.However,liver toxicity of these compounds has impeded their clinical application and the potential hepatotoxicity mechanisms remain to be elucidated.In this work,we found that PPI and PII exposure could induce significant hepatotoxicity in human liver cell line L-02 and zebrafish in a dose-dependent manner.The results of the proteomic analysis in L-02 cells and transcriptome in zebrafish indicated that the hepatotoxicity of PPI and PII was associated with the cholesterol biosynthetic pathway disorders,which were alleviated by the cholesterol biosynthesis inhibitor lovastatin.Additionally,3-hydroxy-3-methy-lglutaryl CoA reductase(HMGCR)and squalene epoxidase(SQLE),the two rate-limiting enzymes in the cholesterol synthesis,selected as the potential targets,were confirmed by the molecular docking,the overexpression,and knockdown of HMGCR or SQLE with siRNA.Finally,the pull-down and surface plasmon resonance technology revealed that PPI could directly bind with SQLE but not with HMGCR.Collectively,these data demonstrated that PPI-induced hepatotoxicity resulted from the direct binding with SQLE protein and impaired the sterol-regulatory element binding protein 2/HMGCR/SQLE/lanosterol synthase pathways,thus disturbing the cholesterol biosynthesis pathway.The findings of this research can contribute to a better understanding of the key role of SQLE as a potential target in drug-induced hepatotoxicity and provide a therapeutic strategy for the prevention of drug toxic effects with similar structures in the future.Zhiqi Li Qiqi Fan Meilin Chen Ying Dong Farong Li Mingshuang Wang Yulin Gu Simin Guo Xianwen Ye Jiarui Wu Shengyun Dai Ruichao Lin Chongjun Zhao 2023Journal of Pharmaceutical Analysis2023,13,1:2
10折合频率对三维跨声速压气机颤振稳定性的影响显示文摘It is well known that fan/compressor blade flutter stability increases with the increase of reduced frequency.Less well-known is that the least stable inter blade phase angle(IBPA)increases with the drop of reduced frequency.However,it is quite striking that little can be found in the open literature about the mechanism to the observations.In this paper,a numerical investigation is carried out to uncover the mechanism of the effect of reduced frequency on flutter stability and the least stable IBPA.The NASA rotor 67 has been used as the test vehicle with its first bending and torsion modes being considered.The time domain harmonic balance method together with the influence coefficient method is used to obtain the worksum-IBPA curves for all cases.It is found that:1)the deterioration of flutter stability with the decrease of reduced frequency is dictated by the dominant decrease of aerodamping due to a blade own vibration;2)the increase of the least stable IBPA with the decrease of reduced frequency arises largely from the increase of the least stable IBPA of the aerodamping from the nearest blade on a blade pressure side.Ruichao Shi Yufang Li Dingxi Wang 2022风机技术2022,64,3:2
11Increases in Anthropogenic Heat Release from Energy Consumption Lead to More Frequent Extreme Heat Events in Urban Cities显示文摘With economic development and rapid urbanization,increases in Gross Domestic Product and population in fastgrowing cities since the turn of the 21st Century have led to increases in energy consumption.Anthropogenic heat flux released to the near-surface atmosphere has led to changes in urban thermal environments and severe extreme temperature events.To investigate the effects of energy consumption on urban extreme temperature events,including extreme heat and cold events,a dynamic representation scheme of anthropogenic heat release(AHR)was implemented in the Advanced Research version of the Weather Research and Forecasting(WRF)model,and AHR data were developed based on energy consumption and population density in a case study of Beijing,China.Two simulations during 1999−2017 were then conducted using the developed WRF model with 3-km resolution with and without the AHR scheme.It was shown that the mean temperature increased with the increase in AHR,and more frequent extreme heat events were produced,with an annual increase of 0.02−0.19 days,as well as less frequent extreme cold events,with an annual decrease of 0.26−0.56 days,based on seven extreme temperature indices in the city center.AHR increased the sensible heat flux and led to surface energy budget changes,strengthening the dynamic processes in the atmospheric boundary layer that reduce AHR heating efficiency more in summer than in winter.In addition,it was concluded that suitable energy management might help to mitigate the impact of extreme temperature events in different seasons.Bin LIU Zhenghui XIE Peihua QIN Shuang LIU Ruichao LI Longhuan WANG Yan WANG Binghao JIA Si CHEN Jinbo XIE Chunxiang SHI 2021Advances in Atmospheric Sciences2021,38,3:2
12Recombinant Human Endostatin Endostar Inhibits Tumor Growth and Metastasis in a Mouse Xenograft Model of Colon Cancer显示文摘Yitao Jia Min Liu Wangang Huang Zhenbao Wang Yutong He Jianhua Wu Shuguang Ren Yingchao Ju Ruichao Geng Zhongxin Li 2012Pathology & Oncology Research2012,,2:1
13Prevalence and characterization of Salmonella species isolated from pigs, ducks and chickens in Sichuan Province, China显示文摘Ruichao Li Jing Lai Yang Wang Shuliang Liu Yun Li Kunyao Liu Jianzhong Shen Congming Wu 2013International Journal of Food Microbiology2013,,1:1
14Physical, mechanical and antimicrobial properties of starch films incorporated with e-poly-L-lysine显示文摘LIMING ZHANG RUICHAO LI FENG DONG 2015Food Chemistry2015,,166:1
15Prevalence and characterization of Salmonella species isolated from pigs,ducks and chickens in Sichuan Province,China显示文摘Ruichao Li Jing Lai Yang Wang 2013Int J Food Microbiol2013,163,1:1
16CAS-LSM Datasets for the CMIP6 Land Surface Snow and Soil Moisture Model Intercomparison Project显示文摘The datasets of the five Land-offline Model Intercomparison Project(LMIP)experiments using the Chinese Academy of Sciences Land Surface Model(CAS-LSM)of CAS Flexible Global-Ocean-Atmosphere-Land System Model Grid-point version 3(CAS FGOALS-g3)are presented in this study.These experiments were forced by five global meteorological forcing datasets,which contributed to the framework of the Land Surface Snow and Soil Moisture Model Intercomparison Project(LS3MIP)of CMIP6.These datasets have been released on the Earth System Grid Federation node.In this paper,the basic descriptions of the CAS-LSM and the five LMIP experiments are shown.The performance of the soil moisture,snow,and land-atmosphere energy fluxes was preliminarily validated using satellite-based observations.Results show that their mean states,spatial patterns,and seasonal variations can be reproduced well by the five LMIP simulations.It suggests that these datasets can be used to investigate the evolutionary mechanisms of the global water and energy cycles during the past century.Binghao JIA Longhuan WANG Yan WANG Ruichao LI Xin LUO Jinbo XIE Zhenghui XIE Si CHEN Peihua QIN Lijuan LI Kangjun CHEN 2021Advances in Atmospheric Sciences2021,38,5:1
17Energy-Efficient Deployment of Water Quality Sensor Networks显示文摘Water quality sensor networks are promising tools for the exploration of oceans.Some key areas need to be monitored effectively.Water quality sensors are deployed randomly or uniformly,however,and understanding how to deploy sensor nodes reasonably and realize effective monitoring of key areas on the basis of monitoring the whole area is an urgent problem to be solved.Additionally,energy is limited in water quality sensor networks.When moving sensor nodes,we should extend the life cycle of the sensor networks as much as possible.In this study,sensor nodes in non-key monitored areas are moved to key areas.First,we used the concentric circle method to determine the mobile sensor nodes and the target locations.Then,we determined the relationship between the mobile sensor nodes and the target locations according to the energy matrix.Finally,we calculated the shortest moving path according to the Floyd algorithm,which realizes the redeployment of the key monitored area.The simulation results showed that,compared with the method of direct movement,the proposed method can effectively reduce the energy consumption and save the network adjustment time based on the effective coverage of key areas.Qian Sun Zhiping Shen Jinglin Liang Xiaoyi Wang Jiping Xu Li Wang Huiyan Zhang Jiabin Yu Ning Cao Ruichao Wang 2021Computers, Materials & Continua2021,,9:0
18Erratum to Clinical management and survival outcomes of patients with different molecular subtypes of diffuse gliomas in China(2011-2017):a multicenter retrospective study from CGGA显示文摘In the published version of Figure 21,an error appeared in Figure 2C on page 1468.In Figure 2C,the Kaplan-Meier estimation of the overall survival of patients with recurrent DG classified according to molecular subtypes was mistakenly covered by the curves of patients with primary DGs during the figure layout process,while the number statistic under the figure is correct.Figure 2C has been updated to correct this mistake.The error does not affect the conclusions of this article.We apologize for the error and for any confusion that it might have caused.Kenan Zhang Xing Liu Guanzhang Li Xin Chang Shouwei Li Jing Chen Zheng Zhao Jiguang Wang Tao Jiang Ruichao Chai 2023Cancer Biology & Medicine2023,20,7:0
19Similarity phenomena of lean swirling flames at different bulk velocities with acoustic disturbances显示文摘In this study,flame responses to acoustic disturbances with different frequencies and amplitudes were experimentally investigated in a lean premixed swirl-stabilized combustor operating at different bulk velocities.The total heat release rate fluctuations and spatial CH*chemiluminescence distributions were captured using a photomultiplier tube and high-speed camera,respectively.The results indicate that the heat release rate exhibits a relatively drastic oscillation and high-order harmonics for low-frequency disturbances.When the bulk velocity and forcing frequency were doubled simultaneously,similar flame structures were observed in the CH*chemiluminescence distributions.As the bulk velocity increases,the gain of the Flame Describing Function(FDF)extends toward the higher frequencies,and the delay time of the flame response decreases.The similarity among FDFs at different bulk velocities was effectively captured by introducing a non-dimensional parameter,defined as the ratio of the flame response delay to the forcing time scale,to replace the dimensional forcing frequency.Furthermore,the availability of the newly defined non-dimensional parameter was verified for flames with different swirl numbers,as this played an important role in determining the flame structures and associated unsteady heat release rate.Zhuming RAO Ruichao LI Peizhe ZHAO Bing WANG Dan ZHAO Qiaofeng XIE 2023Chinese Journal of Aeronautics2023,36,5:0
20Effects of inter-layer remelting frequency on the microstructure evolution and mechanical properties of equimolar CoCrFeNiMn high entropy alloys during in-situ powder-bed arc additive manufacturing(PBAAM)process显示文摘An equiatomic CoCrFeNiMn High Entropy Alloy(HEA)was in-situ deposited by the powder-bed arc ad-ditive manufacturing(PBAAM)process for the first time.Comparative research was conducted on the evolution of phase,crystallographic orientation,dislocation morphology,precipitation,and mechanical performance with the accumulation of inter-layer remelting times.The experimental outcomes mani-fested that the PBAAMed CoCrFeNiMn HEA consists of a stable solid-solution FCC structure,with de-creased lattice parameter but slightly increased(full width at half maximum)FWHM as the accumulation of the inter-layer remelting.The{001}<100>cube texture with a weakened texture intensity was de-tected with an increment of inter-layer remelting frequency from once to 5 times,yet it was transformed into{011}<100>Goss texture with a further increase to 7 times.Additionally,the mean grain diameter distinctly decreased,while the volume fraction of(low angle grain boundaries)LAGBs and dislocation density remarkably added up as the accumulated inter-layer remelts.Predominant cellular substructure generated in all process conditions and could be easily differentiated by elemental segregation.Both theσand M 23 C 6 Cr-rich precipitates in nano-scale and submicron MnS precipitate were detected on the grain boundaries of the PBAAMed deposited components,with a rather sparse distribution.Speaking of mechanical performance,the YS,UTS,and hardening rate are generally increased while the UE is grad-ually decreased as increased inter-layer remelting times.The studied PBAAMed CoCrFeNiMn HEA pos-sesses comparable mechanical performances with the counterparts of laser-deposited and as-cast ones.The strengthening mechanisms of the studied material are predominantly the grain boundary strength-ening and dislocation strengthening.This investigation would be a valuable resource in the research field of fabricating HEA alloys with acceptable microstructure and properties using the PBAAM method.Jun Wang Yao Lu Fanghui Jia Wenzhen Xia Fei Lin Jian Han Ruichao Wang Zengxi Pan Huijun Li Zhengyi Jiang 2022Journal of Materials Science & Technology2022,,18:0
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