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| 1 | Generation and characterization of stable pig pregastrulation epiblast stem cell lines显示文摘Pig epiblast-derived pluripotent stem cells are considered to have great potential and broad prospects for human therapeutic model developme nt and livestock breeding.Despite on going attempts since the 1990s,no stably defined pig epiblast-derived stem cell line has bee n established.Here,guided by in sights from a large-scale sin gle-cell tran scriptome an alysis of pig embryos from embryonic day(E)0 to E14,specifically,the tracing of pluripotency changes during epiblast development,we developed an in vitro culture medium for establishing and maintaining stable pluripotent stem cell lines from pig E10 pregastrulation epiblasts(pgEpiSCs).Enabled by chemical inhibition of WNT-related signaling in combination with growth factors in the FGF/ERK,JAK/STAT3,and Activin/Nodal pathways,pgEpiSCs maintain their pluripotency transcriptome features,similar to those of E10 epiblast cells,and normal karyotypes after more than 240 passages and have the potential to differentiate into three germ layers.Strikingly,ultradeep in situ Hi-C analysis revealed functional impacts of chromatin 3D-spatial associations on the transcriptional regulation of pluripote ncy marker genes in pgEpiSCs.I n practice,we con firmed that pgEpiSCs readily tolerate at least three rounds of successive gene editi ng and gene rated cloned gen e-edited live piglets.Our findings deliver on the long-a nticipated promise of pig pluripote nt stem cells and open new avenues for biological research,animal husbandry,and regenerative biomedicine. | Minglei Zhi Jinying Zhang Qianzi Tang Dawei Yu Shuai Gao Dengfeng Gao Pengliang Liu Jianxiong Guo Tang Hai Jie Gao Suying Cao Zimo Zhao Chongyang Li Xiaogang Weng Mengnan He Tianzhi Chen Yingjie Wang Keren Long Deling Jiao Guanglei Li Jiaman Zhang Yan Liu Yu Lin Daxin Pang Qianqian Zhu Naixin Chen Jingjing Huang Xinze Chen Yixuan Yao Jingcang Yang Zicong Xie Xianya Huang Mengxin Liu Ran Zhang Qiuyan Li Yiliang Miao Jianhui Tian Xingxu Huang Hongsheng Ouyang Bofeng Liu Wei Xie Qi Zhou Zhonghua Liu Caihong Zheng Mingzhou Li Jianyong Han | 2022 | Cell Research2022，32，4： | 3 |
| 2 | Two Inhibitors Against the 3C-Like Proteases of Swine Coronavirus and Feline Coronavirus显示文摘Coronaviruses(CoVs)are important human and animal pathogens that cause respiratory and gastrointestinal diseases.Porcine epidemic diarrhoea(PED),characterized by severe diarrhoea and vomiting in pigs,is a highly lethal disease caused by porcine epidemic diarrhoea virus(PEDV)and causes substantial losses in the swine industry worldwide.However,currently available commercial drugs have not shown great therapeutic effects.In this study,a fluorescence resonance energy transfer(FRET)-based assay was applied to screen a library containing 1,590 compounds and identified two compounds,3-(aminocarbonyl)-1-phenylpyridinium and 2,3-dichloronaphthoquinone,that target the 3C-like protease(3CL^(pro))of PEDV.These compounds are of low molecular weight(MW)and greatly inhibited the activity of this enzyme(IC_(50) values were obtained in this study).Furthermore,these compounds exhibited antiviral capacity against another member of the CoV family,feline infectious peritonitis virus(FIPV).Here,the inhibitory effects of these compounds against CoVs on Vero cells and feline kidney cells were identified(with EC_(50) values)and cell viability assays were performed.The results of putative molecular docking models indicate that these compounds,labeled compound 1 and compound 2,contact the conserved active sites(Cys144,Glu165,Gln191)of 3CL^(pro) via hydrogen bonds.These findings provide insight into the antiviral activities of compounds 1 and 2 that may facilitate future research on anti-CoV drugs. | Mengxin Zhou Yutong Han Mengxia Li Gang Ye Guiqing Peng | 2021 | Virologica Sinica2021，36，6： | 1 |
| 3 | Multilevel coverage location model of earthquake relief material storage repository considering distribution time sequence characteristics显示文摘The scientific location of earthquake emergency supply warehouses is conducive to the effective distribution of emergency relief resources and improved rescue efficiency in earthquake hazard. Comprehensively considering the regional population as well as coverage quality at the demand points, this paper aims to divide the coverage thresholds of earthquake emergency rescue and logistic supplies according to their time-series features,and to build a location model for supply warehouses according to the variety and amount of stored supplies considering their time-series features, in hope of optimizing the set covering issue of earthquake relief supply warehouses. The solution is approached with two methods: the target deviation rate minimization model and NSGA-Ⅱ algorithm. The results obtained by solving the target deviation rate minimization model can balance every target. The branch and bound algorithm can find the global optimal solution at a certain calculation scale with high calculation efficiency, but its efficiency decreases significantly when the operation scale increases. The NSGA-Ⅱ algorithm is more suitable for large-scale solution calculations with high calculation efficiency, and it can output a set of non-inferior solutions for decision makers to select from according to different preference. Taking Aba Prefecture in Sichuan Province as illustration, the feasibility of the model is validated;meanwhile, the effectiveness and benefits of the two approaches in solving the problem of multi-objective set covering of the warehouses are compared and analyzed. | Xianjun Guan Han Zhou Mengxin Li Ligang Zhou Huayou Chen | 2021 | Journal of Traffic and Transportation Engineering(English Edition)2021，8，2： | 1 |
| 4 | SUMOylation-triggered ALIX activation modulates extracellular vesicles circTLCD4-RWDD3 to promote lymphatic metastasis of non-small cell lung cancer显示文摘Lymph node(LN)metastasis is one of the predominant metastatic routes of non-small cell lung cancer(NSCLC)and is considered as a leading cause for the unsatisfactory prognosis of patients.Although lymphangiogenesis is well-recognized as a crucial process in mediating LN metastasis,the regulatory mechanism involving lymphangiogenesis and LN metastasis in NSCLC remains unclear.In this study,we employed high-throughput sequencing to identify a novel circular RNA(circRNA),circTLCD4-RWDD3,which was significantly upregulated in extracellular vesicles(EVs)from LN metastatic NSCLC and was positively associated with deteriorated OS and DFS of patients with NSCLC from multicenter clinical cohort.Downregulating the expression of EV-packaged circTLCD4-RWDD3 inhibited lymphangiogenesis and LN metastasis of NSCLC both in vitro and in vivo.Mechanically,circTLCD4-RWDD3 physically interacted with hnRNPA2B1 and mediated the SUMO2 modification at K108 residue of hnRNPA2B1 by upregulating UBC9.Subsequently,circTLCD4-RWDD3-induced SUMOylated hnRNPA2B1 was recognized by the SUMO interaction motif(SIM)of ALIX and activated ALIX to recruit ESCRT-III,thereby facilitating the sorting of circTLCD4-RWDD3 into NSCLC cell-derived EVs.Moreover,EV-packaged circTLCD4-RWDD3 was internalized by lymphatic endothelial cells to activate the transcription of PROX1,resulting in the lymphangiogenesis and LN metastasis of NSCLC.Importantly,blocking EV-mediated transmission of circTLCD4-RWDD3 via mutating SIM in ALIX or K108 residue of hnRNPA2B1 inhibited the lymphangiogenesis and LN metastasis of NSCLC in vivo.Our findings reveal a precise mechanism underlying SUMOylated hnRNPA2B1-induced EV packaging of circTLCD4-RWDD3 in facilitating LN metastasis of NSCLC,suggesting that EV-packaged circTLCD4-RWDD3 could be a potential therapeutic target against LN metastatic NSCLC. | Xiayao Diao Chao Guo Hanhao Zheng Ke Zhao Yuming Luo Mingjie An Yan Lin Jiancheng Chen Yuanlong Li Yuting Li Xuehan Gao Jiaqi Zhang Mengxin Zhou Wenliang Bai Lei Liu Guige Wang Lanjun Zhang Xiaotian He Rusi Zhang Zhihua Li Changhao Chen Shanqing Li | 2023 | Signal Transduction and Targeted Therapy2023，8，12： | 0 |
| 5 | A New Fiber Detection Method for LAMOST Based on the Front-illuminated Method显示文摘The double revolving fiber positioning technology is one of the key technologies for the success of the Large Sky Area Multi-Object Fiber Spectroscopic Telescope(LAMOST).The accuracy of fiber positioning will directly affect the observation efficiency of LAMOST.To achieve higher fiber positioning accuracy,the original open-loop controlled fiber positioning system urgently needs to be upgraded into a closed-loop control system.The fiber detection is the most important part of the closed-loop controlled fiber positioning system.The back-illuminated detection method is usually used to detect the fiber position by directly detecting the light spot generated at the fiber end in the multi-fiber spectral surveys.In this paper,we introduce a new method to measure the fiber position based on the image of the front-illuminated LAMOST focal plane.The front-illuminated image does not require lighting devices inside the spectrograph,and it could reduce the instability and light pollution in the spectrograph end.Our method measures the fiber position by fitting the profile of the fiber pinhole with a 2D Gaussian function.A series of tests show that the relative position measurement precision of the front-illuminated method is about 012,and the method could have the same accuracy as the back-illuminated method once the system bias is calibrated by a simple radial correction function.The required fiber positioning accuracy of LAMOST is 04,and the new method satisfies the requirement of LAMOST fiber detection accuracy and could be used in the closed-loop fiber control system. | Ming Zhou Yong Zhang Guanru Lv Jian Li Zengxiang Zhou Zhigang Liu Jianping Wang Zhongrui Bai Yuan Tian Mengxin Wang Shuqing Wang Hongzhuan Hu Chao Zhai Jiaru Chu Zhijie Han Mingxu Liu Yiqiao Dong Hailong Yuan Yongheng Zhao Yaoquan Chu Haotong Zhang | 2022 | Research in Astronomy and Astrophysics2022，22，6： | 0 |
| 6 | Broad phenotypic alterations and potential dysfunction of lymphocytes in individuals clinically recovered from COVID-19显示文摘Although millions of patients have clinically recovered from COVID-19,little is known about the immune status of lymphocytes in these individuals.In this study,the peripheral blood mononuclear cells of a clinically recovered(CR)cohort were comparatively analyzed with those of an age-and sex-matched healthy donor cohort.We found that CD8^(+)T cells in the CR cohort had higher numbers of effector T cells and effector memory T cells but lower Tc1(IFN-γ^(+)),Tc2(IL-4^(+)),and Tc17(IL-17A^(+))cell frequencies.The CD4^(+)T cells of the CR cohort were decreased in frequency,especially the central memory T cell subset.Moreover,CD4^(+)T cells in the CR cohort showed lower programmed cell death protein 1(PD-1)expression and had lower frequencies of Th1(IFN-γ^(+)),Th2(IL-4^(+)),Th17(IL-17A^(+)),and circulating follicular helper T(CXCR5^(+)PD-1^(+))cells.Accordingly,the proportion of isotype-switched memory B cells(IgM−CD20^(hi))among B cells in the CR cohort showed a significantly lower proportion,although the level of the activation marker CD71 was elevated.For CD3−HLA-DR−lymphocytes in the CR cohort,in addition to lower levels of IFN-γ,granzyme B and T-bet,the correlation between T-bet and IFN-γ was not observed.Additionally,by taking into account the number of days after discharge,all the phenotypes associated with reduced function did not show a tendency toward recovery within 4-11 weeks.The remarkable phenotypic alterations in lymphocytes in the CR cohort suggest that severe acute respiratory syndrome coronavirus 2 infection profoundly affects lymphocytes and potentially results in dysfunction even after clinical recovery. | Jingyi Yang Maohua Zhong Ejuan Zhang Ke Hong Qingyu Yang Dihan Zhou Jianbo Xia Yao-Qing Chen Mingbo Sun Bali Zhao Jie Xiang Ying Liu Yang Han Mengxin Xu Xi Zhou Chaolin Huang You Shang Huimin Yan | 2021 | Journal of Molecular Cell Biology2021，13，3： | 0 |
| 7 | Surface protonation and oxygen evolution activity of epitaxial La(1-x)SrxCoO3 thin films显示文摘As an alternative electrode material,transition metal oxides are promising candidates due to multivalent nature and oxygen vacancies present in the structure with facilitate redox reactions.The aim of this study is to explore the intrinsic mechanism of oxygen evolution reaction(OER)using two-dimensional thin film La1-xSrxCoO3 electrode as a model.Herein,we report a planar two-dimensional model La1-xSrxCoO3 electrode grown on a Nb-SrTiO3 single-crystal substrate via pulsed laser deposition.The two-dimensional La1-xSrxCoO3 films offer different oxygen evolution activities at different pH electrolyte solutions.The mechanisms behind the variations of the oxygen evolution activity were discussed after comparing the oxygen evolution activity before and after treatments of the electrodes and measurements by various test methods.The results of this study offer a promising,low-cost electrode material for the efficient OER and a sustainable production of hydrogen fuel. | XiaoShuo Wang Liang Zhou MengXin Li Yi Luo TieYing Yang TianLi Wu XiaoLong Li Kui-Juan Jin Er-Jia Guo LiFen Wang XueDong Bai WeiFeng Zhang HaiZhong Guo | 2020 | Science China(Physics,Mechanics & Astronomy)2020，63，9： | 0 |
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