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| 1 | Transcriptome analysis provides insights into the regulation of metabolic processes during postharvest cold storage of loquat(Eriobotrya japonica)fruit显示文摘Loquat(Eriobotrya japonica)fruit accumulates lignin during postharvest storage under chilling conditions(0℃),while low-temperature conditioning(LTC;5℃for 6 days followed by transfer to 0℃)or heat treatment(HT;40℃for 4 h followed by transfer to 0℃)can alleviate lignification.Here we compared transcriptome profiles of loquat fruit samples under LTC or HT to those stored at 0℃at five time points from day 1 to day 8 after treatment.High-throughput transcriptome sequences were de novo assembled into 53,319 unique transcripts with an N50 length of 1306 bp.A total of 2235 differentially expressed genes were identified in LTC,and 1020 were identified in HT compared to 0℃.Key genes in the lignin biosynthetic pathway,including EjPAL2,EjCAD1,EjCAD3,4CL,COMT,and HCT,were responsive to LTC or HT treatment,but they showed different expression patterns during the treatments,indicating that different structural genes could regulate lignification at different treatment stages.Coexpression network analysis showed that these candidate biosynthetic genes were associated with a number of transcription factors,including those belonging to the AP2,MYB,and NAC families.Gene ontology(GO)enrichment analysis of differentially expressed genes indicated that biological processes such as stress responses,cell wall and lignin metabolism,hormone metabolism,and metal ion transport were significantly affected under LTC or HT treatment when compared to 0℃.Our analyses provide insights into transcriptome responses to postharvest treatments in loquat fruit. | Wenli Liu Jing Zhang Chen Jiao Xueren Yin Zhangjun Fei Qingbiao Wu Kunsong Chen | 2019 | Horticulture Research2019，6，1： | 3 |
| 2 | Label-free visualization of lignin deposition in loquats using complementary stimulated and spontaneous Raman microscopy显示文摘The lignification triggered by biotic or abiotic stresses hardens fruits and vegetables and eventually influences their consumer appeal.Extensive prior efforts have been made to unveil the underlying mechanism of flesh lignification,primarily focused on its physicochemical and molecular biological properties.Nevertheless,most of these studies used destroyed and homogenized bulk tissues as analytes;as a result,potentially valuable spatial information was lost.In this study,the deposition of lignin in loquat flesh during lignification was visualized from the tissue level to the singlecell level by combining the advantages of stimulated Raman scattering(SRS)and spontaneous Raman microscopy using label-free in situ molecular imaging.SRS has the advantages of being fast and providing large-area chemical imaging to reveal the spatial heterogeneity of lignin and cell wall polysaccharide distribution in loquat flesh.After 2 days of storage at 0℃,increased lignins were observed by large-area SRS imaging.In addition,microscopic SRS images of the flesh cells indicated that the increased lignins were trapped in the cell corner(CC)and middle lamella(ML).Furthermore,the compositional and structural features of lignified cells(LCs),CC and ML of loquat flesh were investigated by spontaneous Raman microscopy,and the results showed that the LCs were a combination of lignin,cellulose,and hemicellulose,whereas CC and ML showed only deposited lignin and pectin without cross-linked cellulose and hemicellulose.This result further suggests that the lignins in the CC and ML regions of loquats were later synthesized alone during postharvest storage.This innovative combination of SRS and spontaneous Raman microscopy allows the label-free macroscale and fine chemical imaging of plant cell walls and will enhance our fundamental understanding of the structures and functions of the plant cell wall. | Nan Zhu Yifan Yang Minbiao Ji Di Wu Kunsong Chen | 2019 | Horticulture Research2019，6，1： | 3 |
| 3 | An assessment of genetic variability and relationships within Asian pears based on AFLP (amplified fragment length polymorphism) markers显示文摘 | Lu Bao Kunsong Chen Dong Zhang Xiugen Li Yuanwen Teng | 2008 | Scientia Horticulturae2008，，4： | 2 |
| 4 | Low temperature conditioning reduces postharvest chilling injury in loquat fruit显示文摘 | Chong Cai ChangJie Xu LanLan Shan Xian Li ChunHua Zhou WangShu Zhang Ian Ferguson KunSong Chen | 2006 | Postharvest Biology and Technology2006，，3： | 2 |
| 5 | Biosynthetic labeling with 3-O-propargylcaffeyl alcohol reveals in vivo cell-specific patterned lignification in loquat fruits during development and postharvest storage显示文摘Lignification is a major cell wall modification that often results in the formation of sophisticated subcellular patterns during plant development or in response to environmental stresses.Precise localization of the spatiotemporal deposition of lignin is of great importance for revealing the lignification regulatory mechanism of individual cells.In loquat fruits,lignification typically increases the flesh lignin content and firmness,reducing their edibility and processing quality.However,the precise localization of the spatiotemporal active zones of lignification inside loquat fruit flesh remains poorly understood,and little is known about the contribution of patterned lignification to cell wall structure dynamics and the subsequent fruit-quality deterioration.Here,we performed an emerging bioorthogonal chemistry imaging technique to trace the in vivo patterned lignification dynamics in cells of loquat fruit flesh during development and storage.In developing fruits,lignified cells(LCs)and vascular bundles(VBs)were the zones of active lignification,and ring-like LCs deposited lignin at both the inner wall layer and the outer periphery sides.The domino effect of the generation of LCs was preliminarily visualized.In mature fruits,the newly formed lignin in the flesh of fruits during storage was specifically deposited in the corners and middle lamellae of parenchyma cells surrounding the VBs,resulting in the development of a reticular structure.Based on the findings,distinct spatiotemporal patterned lignification modes for different flesh cells in loquat fruits were proposed.These findings provide loquat lignification dynamics together with spatiotemporal data that can improve our understanding of the lignification process in planta. | Nan Zhu Chenning Zhao Yuqing Wei Chongde Sun Di Wu Kunsong Chen | 2021 | Horticulture Research2021，8，1： | 2 |
| 6 | Analysis of diversity and relationships among Chinese orchid cultivars using EST-SSR markers显示文摘 | Yan Huang Fang Li Kunsong Chen | 2009 | Biochemical Systematics and Ecology2009，，1： | 1 |
| 7 | Contents and antioxidant capacity of limonin and nornilinin different tissues of citrus fruit of four eultivars during fruit growth and maturation显示文摘 | SUN Chongde CHEN Kunsong CHEN Yang | 2005 | Food Chemistry2005，93，4： | 1 |
| 8 | Contents and antioxidant capacity of limonin and nomilin in dif- ferent tissues of citrus fruit of four cuhivars during fruit growth and maturation 显示文摘 | ChongDe Sun KunSong Chen Yang Chen | 2005 | Food Chemistry2005，93，4： | 1 |
| 9 | Postharvest responses of Chinese bayberry fruit显示文摘 | Wangshu Zhang Kunsong Chen Bo Zhang | 2005 | Postharvest Biology and Technology2005，37，： | 1 |
| 10 | The interference of sucrase in determination of exo-ceUulase and exo-polygalacturonase in abscission zone of citrus young fruit and the methods to exclude it显示文摘 | XU Changjie CFIEN Kunsong ZFIANG Shanglong | 1997 | Plant Physiology Communication1997，33，1： | 1 |
| 11 | Ethylene†and fruit softening显示文摘This review is concerned with the mechanisms controlling fruit softening.Master genetic regulators switch on the ripening programme and the regulatory pathway branches downstream,with separate controls for distinct quality attributes such as colour,flavour,texture,and aroma.Ethylene plays a critical role as a ripening hormone and is implicated in controlling different facets of ripening,including texture change,acting through a range of transcriptional regulators,and this signalling can be blocked using 1-methylcyclopropene.A battery of at least seven cell-wall-modifying enzymes,most of which are synthesized de novo during ripening,cause major alterations in the structure and composition of the cell wall components and contribute to the softening process.Significant differences between fruits may be related to the precise structure and composition of their cell walls and the enzymes recruited to the ripening programme during evolution.Attempts to slow texture change and reduce fruit spoilage by delaying the entire ripening process can often affect negatively other aspects of quality,and low temperatures,in particular,can have deleterious effects on texture change.Gene silencing has been used to probe the function of individual genes involved in different aspects of ripening,including colour,flavour,ethylene synthesis,and particularly texture change.The picture that emerges is that softening is a multi-genic trait,with some genes making a more important contribution than others.In future,it may be possible to control texture genetically to produce fruits more suitable for our needs. | Greg Tucker Xueren Yin Aidi Zhang MiaoMiao Wang Qinggang Zhu Xiaofen Liu Xiulan Xie Kunsong Chen Don Grierson | 2017 | Food Quality and Safety2017，1，4： | 1 |
| 12 | The role of salicylic acid in postharvest ripening of kiwi fruit显示文摘 | ZHANG Yu CHEN Kunsong ZHANG Shanglong | 2003 | Postharvest Biology and Technology2003，28，1： | 1 |
| 13 | Postharvest responses of chinese bayberry fruit显示文摘 | ZhangWangshu Chen Kunsong ZhangBo | 2005 | Postharvest Biology and Technology2005，37，3： | 1 |
| 14 | Effect of 1-MCP on postharvest quality of loquat fruit显示文摘 | Cai Chong Chen Kunsong Xu Wenping | 2006 | Postharvest Biology and Technology2006，40，2： | 1 |
| 15 | Identification and characterization of transcripts differentially ex-pressed in peel and juice vesicle of immature and ripe orange(Citrus sinensis)fruits 显示文摘 | ZHANG Lanlan CHEN Kunsong XU Changjie | 2008 | Plant Mol Biol Rep2008，26，2： | 1 |
| 16 | Contents and antioxidant capacity of limonin and nomilin in different tissues of citrus fruit of four cultivars during fruit growth and maturation显示文摘 | SUN Chongde CHEN Kunsong CHEN Yang | 2005 | Food Chemistry2005，93，4： | 1 |
| 17 | Accumulation of lignin in relation to change in activities of lignification enzymes in loquat fruit flesh after harvest显示文摘 | Chong Cai ChangJie Xu Xian Li Ian Ferguson KunSong Chen | 2006 | Postharvest Biology and Technology2006，，2： | 1 |
| 18 | Contents and antioxidant capacity of limonin and nomilin in different tissues of citrus fruit of four eultivars during fruit growth and maturation显示文摘 | Sun Chongde Chen Kunsong Chen Yang | 2005 | Food Chem2005，93，4： | 1 |
| 19 | Clay sediment - structure formarion in aqueous kanlinite suspensions 显示文摘 | Kunsong Ma Alain C Pierre | 1999 | Clays and clay minerals1999，，4： | 1 |
| 20 | Contents and antioxidant capacity of limonin and nomilin in different tissues of citrus fruit of four eultivars during fruit growth and maturation显示文摘 | ChongDe Sun KunSong Chen Yang Chen | 2005 | Food Chemistry2005，93，4： | 1 |
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