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| 1 | Chinese Stroke Association guidelines for clinical management of cerebrovascular disorders: executive summary and 2019 update of clinical management of ischaemic cerebrovascular diseases显示文摘Aim Stroke is the leading cause of disability and death in China.Ischaemic stroke accounts for about 60%-80%of all strokes.It is of considerable significance to carry out multidimensional management of ischaemic cerebrovascular diseases.This evidence-based guideline aims to provide the latest detailed and comprehensive recommendations on the diagnosis,treatment and secondary prevention of ischaemic cerebrovascular diseases.Methods We had performed comprehensive searches of MEDLINE(via PubMed)(before 30 June 2019),and integrated the relevant information into charts and distributed to the writing group.Writing group members discussed and determined the recommendations through teleconference.We used the level of evidence grading algorithm of Chinese Stroke Association to grade each recommendation.The draft was reviewed by the Guideline Writing Committee of Chinese Stroke Association Stroke and finalised.This guideline is fully updated every 3 years.results This evidence-based guideline is based on the treatment,care and prevention of ischaemic cerebrovascular diseases,which emphasises on pathogenesis evaluation,intravenous thrombolysis,endovascular therapy,antiplatelet therapy,prevention and treatment of complications,and risk factor management.Conclusions This updated guideline presents a framework for the management of ischaemic cerebrovascular diseases.Timely first-aid measures,professional care in the acute stage,and proactive secondary prevention will be helpful to patients. | Liping Liu Weiqi Chen Hongyu Zhou Wanying Duan Shujuan Li Xiaochuan Huo Weihai Xu Li'an Huang Huaguang Zheng Jingyi Liu Hui Liu Yufei Wei Jie Xu Yongjun Wang | 2020 | Stroke & Vascular Neurology2020，5，2： | 42 |
| 2 | Arginine, N-carbamylglutamate,and glutamine exert protective effects against oxidative stress in rat intestine显示文摘The objective of the current study is to evaluate the effects of dietary supplementation with arginine(ARG), N-carbamylglutamate(NCG), and glutamine(GLN) on rat intestinal morphology and antioxidant status under oxidative stress. Rats were fed for 30 d with one of the following iso-nitrogenous diets:basal diet(BD), BD plus 1% ARG, BD plus 0.1% NCG, and BD plus 1% GLN. On day 28, half of the rats fed BD were intraperitoneally injected with 12 mg/kg body weight of diquat(DT; i.e., the DT group) and the other half was intraperitoneally injected with sterile solution(i,e., the control group). The other diet groups were intraperitoneally injected with 12 mg/kg body weight of DT(i,e., DT + 1% GLN [DT + GLN],DT + 1% ARG [DT + ARG], and DT + 0.1% NCG [DT + NCG]). Rat jejunum samples obtained at 48 h after DT injection were analyzed. Results showed that DT significantly decreased catalase(CAT) activity and glutathione(GSH) content by 58.25% and 56.57%, respectively, and elevated malondialdehyde(MDA)content and crypt depth(CD) by 19.39% and 22.13%, respectively, in the jejunum(P < 0.05, relative to the control group). Compared with the DT group, the DT + GLN group exhibited significantly improved villus height(VH), villus width(VW), villus surface area(VSA), CD and total antioxidant capacity(T-AOC) activity(P < 0.05); the DT + ARG group exhibited significantly increased the ratio of VH to CD(H:D) and TAOC activity(P < 0.05); the DT + GLN, DT + ARG and DT + NCG groups exhibited significantly enhanced CAT activity and GSH content as well as decreased MDA content(P < 0.05). Moreover, VH, VW, VSA, CD and GSH content in the DT + GLN group were higher whereas MDA content was lower compared with the corresponding values observed in both the DT + ARG and the DT + NCG groups(P < 0.05). The H:D ratio in the DT + ARG group significantly increased compared with that in the DT + NCG and DT + GLN groups(P < 0.05). Collectively, this study suggested that dietary supplementation with 1% GLN, 0.1% NCG,and 1% ARG was effective in enhancing the antioxidant status and maintaining the morphological structure of rat jejunum under oxidative stress; of these supplements, 1% GLN exerted the greatest effects on mitigating oxidative stress. | Liang Xiao Wei Cao Guangmang Liu Tingting Fang Xianjian Wu Gang Jia Xiaoling Chen Hua Zhao Jing Wang Caimei Wu Jingyi Cai | 2016 | Animal Nutrition2016，，3： | 12 |
| 3 | Endovascular treatment with or without intravenous alteplase for acute ischaemic stroke due to basilar artery occlusion显示文摘Background and purpose It remains controversial if endovascular treatment(EVT)can improve the outcome of patients with acute basilar artery occlusion(BAO).This study aims to compare the functional outcomes between EVT with and without intravenous thrombolysis(IVT)first in patients who had acute ischaemic stroke(AIS)due to BAO.Methods Patients who had AIS with BAO who underwent EVT within 24 hours of onset were enrolled in this multicentre cohort study,and the efficacy and safety were compared between IVT+EVT and direct EVT.The primary outcome was 90-day functional independence.All outcomes were assessed with adjusted OR(aOR)from the multivariable logistic regression.In addition,a meta-analysis was performed on all recently published pivotal studies on functional independence after EVT in patients with BAO.Results Of 310 enrolled patients with BAO,241(78%)were treated with direct EVT and 69(22%)with IVT+EVT.Direct EVT was associated with a worse functional outcome(aOR,0.46(95%CI 0.24 to 0.85),p=0.01).IVT+EVT was associated with a lower percentage of patients who needed≥3 passes of stent retriever(10.14%vs 20.75%).The meta-analysis regression revealed a potential positive correlation between bridging with IVT first and functional independence(r=0.14(95%CI 0.05 to 0.24),p<0.01).Conclusions This study showed that compared with direct EVT,EVT with IVT first was associated with better functional outcomes in patients with BAO treated within 24 hours of onset.The meta-analysis demonstrated similar favourable efficacy of IVT first followed by EVT in patients with BAO. | Ximing Nie David Wang Yuehua Pu Yufei Wei Qixuan Lu Hongyi Yan Xin Liu Lina Zheng Jingyi Liu Xinxuan Yang Yarong Ding Dacheng Liu Wanying Duan Zhe Zhang Zhonghua Yang Miao Wen Weibin Gu Xinyi Hou Xinyi Leng Yuesong Pan Zhongrong Miao Liping Liu | 2022 | Stroke & Vascular Neurology2022，7，3： | 10 |
| 4 | Over-activation of TLR5 signaling by high-dose flagellin induces liver injury in mice显示文摘Flagellin 是涉及天生、适应的免疫的大量房间类型的有势力使活跃之物。因此,它是疫苗的一个好辅助候选人,并且它可能在癌症放射疗法和放射紧急情况的一个缓和者期间对主要尖锐放射症候群作为生物杀虫撒布剂工作。然而,积累证据含有在一些煽动性的疾病的出现的 flagellin 例如尖锐的肺发炎,心血管的倒塌和煽动性的肠疾病。这研究的目的是阐明 flagellin-TLR5 发信号激活是否仅仅或是否在肝的 pathophysiology 起一个作用某另外的 flagellin 活动也经由细菌的感染或在临床的应用期间贡献肝损害。Recombinant flagellin 蛋白质与或没有刺激 TLR5 活动被用来评估 flagellin-TLR5 在肝损害发信号在的角色野类型并且 TLR5 击倒老鼠。粗野损害和 hepatocellular 坏死的大区域在 100 或 200 µ 的 intraperitoneal 管理以后在肝织物 12 h 被观察;在一个剂量依赖者的 g flagellin (警察) 和在野类型的老鼠,然而并非在 TLR5 击倒老鼠的时间依赖者举止。flagellin 的 N 终端或 TLR5 绑定域的删除禁止了导致 flagellin 的煽动性的回答和随后的尖锐的肝函数反常和损坏。这些数据证实 flagellin 是肝损害的一个必要决定因素并且证明由高剂量的 flagellin 发信号的 TLR5 的在激活上在肝引起了尖锐煽动性的回答,嗜中性的累积和氧化应力,它贡献导致 flagellin 的肝损害的前进和严厉。 | Yang Xiao Fang Liu Jingyi Yang Maohua Zhong Ejuan Zhang Yaoming Li Dihan Zhou Yuan Cao Wei Li Jie Yu Yi Yang Huimin Yan | 2015 | Cellular & Molecular Immunology2015，12，6： | 9 |
| 5 | XBP-1u suppresses autophagy by promoting the degradation of FoxO1 in cancer cells显示文摘Autophagy 被激活响应滋养的饥饿维持细胞的精力动态平衡。然而, autophagy 固执地没被激活,它糟糕在机械学的水平被理解。这里,我们报导 FoxO1 的那周转涉及夫酸安饥饿引起的动态 autophagic 过程。X-box-binding protein-1u (XBP-1u ) 由招募在 FoxO1 降级有一个关键角色 FoxO1 到 20S proteasome。另外,由 Ser61 和 Ser176 上的细胞外的调整蛋白质 kinases1/2 (ERK1/2 ) 的 XBP-1u 的 phosphorylation 被发现在夫酸安饥饿之上为在 XBP-1u 和 FoxO1 之间的增加的相互作用批评。而且,引起 FoxO1 的持续水平和 autophagy 的坚持的激活的 XBP-1u 击倒,在房间生存能力导致重要减少。最后,在 XBP-1u 之间的反的关联和 FoxO1 表示同意很好,表示侧面在许多人的癌症纸巾观察了。因此,我们的调查结果连接到 XBP-1u-induced FoxO1 的 autophagy 的动态过程降级。 | Ying Zhao Xue Li Mu-Yan Cai Ke Ma Jing Yang Jingyi Zhou Wan Fu Fu-Zheng Wei Lina Wang Dan Xie Wei-Guo Zhu | 2013 | Cell Research2013，23，4： | 8 |
| 6 | Discovery of Lorandite TlAsS_2 at the Distal Au-Tl Deposit in a Skarn system,Fengshan Area,Middle-Lower Yangtze River,Eastern China显示文摘Objective Thallium has been used as an indicator element in geochemical exploration of searching for hydrothermal gold deposits.However,the T1 minerals and mineralization are rare in nature.Lorandite TlAsS_2,a relatively uncommon mineral,has been dominantly discovered in some Carlin gold deposits,and minor SbHg,U and Pb-Zn-Ag deposits.It is of a hydrothermal origin and was typically formed at relatively low temperatures.The relationships between these deposits containing lorandite and intrusions remain a | XIE Guiqing MAO Jingwen HAN Yingxiao JIAN Wei HAN Jingyi | 2017 | Acta Geologica Sinica(English Edition)2017，91，4： | 7 |
| 7 | Successful Production of an All-Female Common Carp (Cyprinus carpio L.) Population Using cyp17a1-Deficient Neomale Carp显示文摘Due to sexual dimorphism in the growth of certain cultured fish species,the production of monosex fishes is desirable for the aquaculture industry.Nowadays,the most widely practiced technique available for the mass production of monosex fish populations is sex steroid-induced sex reversal.Here,a novel strategy for the successful production of all-female(AF)common carp(Cyprinus carpio L.),to take advantage of the sexual dimorphism in growth documented in this species,has been developed using genetic engineering via single gene-targeting manipulation without any exogenous hormone treatments.Male and female heterozygous cyp17a1-deficient common carp were first obtained using the clustered regularly interspaced short palindromic repeats/CRISPR-associated endonuclease 9(CRISPR/Cas9)technique.An all-male phenotype for homozygous cyp17a1-deficient carp,regardless of the individuals’sexdetermination genotypes(XY or XX),has been observed.A male-specific DNA marker newly identified in our laboratory was used to screen the neomale carp population with the XX genotype from the cyp17a1-deficient carp.These neomale carp develop a normal testis structure with normal spermatogenesis and sperm capacity.The neomale common carp were then mated with wild-type(WT)females(cyp17a1^(+/+)XX genotype)using artificial fertilization.All the AF offspring sample fish from the neomale-WT female mating were confirmed as having the cyp17a^(1+/-)XX genotype,and normal development of gonads to ovaries was observed in 100.00%of this group at eight months post-fertilization(mpf).A total of 1000 carp fingerlings,500 from the WT male and female and 500 from the neomale and WT female mating,were mixed and reared in the same pond.The average body weight of cyp17a1^(+/-)XX females was higher by 6.60%(8 mpf)and 32.66%(12 mpf)than that of the control common carp.Our study demonstrates the first successful production of a monosex teleost population with the advantages of sexual dimorphism in growth using genetic manipulation targeting a single locus. | Gang Zhai Tingting Shu Kuangxin Chen Qiyong Lou Jingyi Jia Jianfei Huang Chuang Shi Xia Jin Jiangyan He Donghuo Jiang Xueqiao Qian Wei Hu Zhan Yin | 2022 | Engineering2022，8，1： | 7 |
| 8 | The RNA-binding protein QKI5 regulates primary miR-124-1 processing via a distal RNA motif during erythropoiesis显示文摘 | Fang Wang Wei Song Hongmei Zhao Yanni Ma Yuxia Li Di Zhai Jingnan Pi Yanmin Si Jiayue Xu Lei Dong Rui Su Mengmeng Zhang Yong Zhu Xiaoxia Ren Fei Miao Wenjie Liu Feng Li Junwu Zhang Aibin He Ge Shan Jingyi Hui Linfang Wang Jia Yu | 2017 | Cell Research2017，27，3： | 7 |
| 9 | An imine-linked covalent organic framework as the host material for sulfur loading in lithium–sulfur batteries显示文摘Lithium–sulfur(Li–S) batteries have high theoretical specific capacity, providing new opportunities for the next generation of secondary battery. Covalent organic framework(COF) as a new porous crystalline material has been used as the host material in Li–S battery to improve the cell's cycling stability. In this paper, an imine-linked TAPB-PDA-COF was applied as the host material for sulfur loading(60%) in Li–S battery. The TAPB-PDA-COF has a beehive-like morphology with high thermal stability(up to 500 ℃).In the electrochemical experiment, the performance of the composite cathode with acetylene black(AB) and super-P(S-P) as the conductive additives was studied individually. The initial discharge capacity under 0.2 A/g current density was 991 mAh/g and 1357 mAh/g for TAPB-PDA-COF/S@A-B and TAPB-PDACOF/S@S-P, respectively. The better result of S-P based cathode than A-B could be due to the better conductivity of the S-P, as proved by the EIS results. When further increased the current density to 2 A/g,the S-P based composite cathode can still deliver a comparable initial discharge capacity of 630 and 274 mAh/g capacity remained after 940 cycles. This results will inspire researchers develop more suitable conductive additives together with the host materials for high performance Li–S battery. | Jianyi Wang Liping Si Qin Wei Xujia Hong Ligui Lin Xin Li Jingyi Chen Peibiao Wen Yuepeng Cai | 2019 | Journal of Energy Chemistry2019，28，1： | 7 |
| 10 | NLRC4 downregulates 的激活对 flagellin 的调停 TLR5 的抗体免疫者回答显示文摘细菌的 flagellin 是一个唯一的联系病原体的分子的模式(PAMP ) ,它能被表面认出局部性的像使用费的受体 5 (TLR5 ) 并且 cytosolic 像点头的受体(NLR ) 蛋白质 4 (NLRC4 ) 受体。由 flagellin 和产生有免疫力的回答戏表明小径的 TLR5 或 NLRC4 的激活在抗菌剂免疫的重要角色。然而,它仍然保持不清楚怎么 flagellin 的双活动激活 TLR5 或 NLRC4 发信号小径安排有免疫力的回答。在这研究,我们估计了缺乏能力对 flagellin 在适应有免疫力的回答上独自或在联合激活 TLR5 和 NLRC4 的 flagellin 和它的异种的效果。是不能的激活 NLRC4 的 Flagellin 比的导致了显著地更高的抗体回答野类型的 flagellin。当巨噬细胞在 vivo 被弄空时,增加的抗体反应能被消除。由 flagellin downregulated 的 NLRC4 的激活对 flagellin 的导致 flagellin 、调停 TLR5 的有免疫力的回答。 | Wei Li Jingyi Yang Ejuan Zhang Maohua Zhong Yang Xiao Jie Yu Dihan Zhou Yuan Cao Yi Yang Yaoming Li Huimin Yan | 2016 | Cellular & Molecular Immunology2016，13，4： | 5 |
| 11 | The landscape of aging显示文摘Aging is characterized by a progressive deterioration of physiological integrity,leading to impaired functional ability and ultimately increased susceptibility to death.It is a major risk factor for chronic human diseases,including cardiovascular disease,diabetes,neurological degeneration,and cancer.Therefore,the growing emphasis on “healthy aging” raises a series of important questions in life and social sciences.In recent years,there has been unprecedented progress in aging research,particularly the discovery that the rate of aging is at least partly controlled by evolutionarily conserved genetic pathways and biological processes.In an attempt to bring full-fledged understanding to both the aging process and age-associated diseases,we review the descriptive,conceptual,and interventive aspects of the landscape of aging composed of a number of layers at the cellular,tissue,organ,organ system,and organismal levels. | Yusheng Cai Wei Song Jiaming Li Ying Jing Chuqian Liang Liyuan Zhang Xia Zhang Wenhui Zhang Beibei Liu Yongpan An Jingyi Li Baixue Tang Siyu Pei Xueying Wu Yuxuan Liu Cheng-Le Zhuang Yilin Ying Xuefeng Dou Yu Chen Fu-Hui Xiao Dingfeng Li Ruici Yang Ya Zhao Yang Wang Lihui Wang Yujing Li Shuai Ma Si Wang Xiaoyuan Song Jie Ren Liang Zhang Jun Wang Weiqi Zhang Zhengwei Xie Jing Qu Jianwei Wang Yichuan Xiao Ye Tian Gelin Wang Ping Hu Jing Ye Yu Sun Zhiyong Mao Qing-Peng Kong Qiang Liu Weiguo Zou Xiao-Li Tian Zhi-Xiong Xiao Yong Liu Jun-Ping Liu Moshi Song Jing-Dong J.Han Guang-Hui Liu | 2022 | Science China(Life Sciences)2022，65，12： | 4 |
| 12 | Preparation and application of a phosphorous free and non- nitrogen scale inhibitor in industrial cooling water systems显示文摘钙碳酸盐,锌(II ) 和铁(III ) 的一种新奇环境地友好的类型规模禁止者丙烯酸的 acidallylpolyethoxy 羧化物共聚物(AAAPEL ) 被综合。向 CaCO 的 AA-APEL 的反规模性质 < 潜水艇 class= “ a-plus-plus ” > 3 , 锌(II ) 和人工的冷却水里的铁(III ) 通过静态的规模抑制测试被学习。观察证明两钙碳酸盐,锌(II ) 和铁(III ) 抑制与增加 AA-APEL 的剂量增加。CaCO 的形成上的效果 < 潜水艇 class= “ a-plus-plus ” > 3 与扫描电子显微镜学(SEM ) 的联合被调查,传播电子显微镜学(TEM ) , X 光检查粉末衍射(XRD ) 分析和 fourier 分别地转变红外线的分光计。结果证明 AA-APEL 共聚物不仅影响的钙碳酸盐水晶形态学和水晶尺寸而且 crystallinity。CaCO 的结晶化 < 潜水艇 class= “ a-plus-plus ” > 当禁止者不在时的 3 是菱形的方解石水晶,而有 vaterite 晶体的方解石的混合物面对 AAAPEL 共聚物被发现。抑制机制被建议在钙或铁离子和聚乙烯乙二醇(木钉) 之间的相互作用是基本动力在使水系统凉下来制止规模的形成。 | Guangqing LIU Mengwei XUE Jingyi HUANG Huchuan WANG Yuming ZHOU Qingzhao YAO Lei LING Ke CAO Yahui LIU Yunyun BU Yiyi CHEN Wendao WU Wei SUN | 2015 | Frontiers of Environmental Science & Engineering2015，9，3： | 3 |
| 13 | Calcium phosphate nanoparticles show an effective activation of the innate immune response in vitro and in vivo after functionalization with flagellin显示文摘For subunit vaccines,adjuvants play a key role in shaping the magnitude,persistence and form of targeted antigen-specific immune response.Flagellin is a potent immune activator by bridging innate inflammatory responses and adaptive immunity and an adjuvant candidate for clinical application.Calcium phosphate nanoparticles are efficient carriers for different biomolecules like DNA,RNA,peptides and proteins.Flagellin-functionalized calcium phosphate nanoparticles were prepared and their immunostimuiatory effect on the innate immune system,i.e.the cytokine production,was studied.They induced the production of the proinflammatory cytokines IL-8(Caco-2 cells) and IL-1β(bone marrow-derived macrophages;BMDM) in vitro and IL-6 in vivo after intraperitoneal injection in mice.The immunostimulation was more pronounced than with free flagellin. | Diana Kozlova Viktoriya Sokolova Maohua Zhong Ejuan Zhang Jingyi Yang Wei Li Yi Yang Jan Buer Astrid Maria Westendorf Matthias Epple Huimin Yan | 2014 | Virologica Sinica2014，29，1： | 3 |
| 14 | A review on perfluoroalkyl acids studies:Environmental behaviors,toxic effects,and ecological and health risks显示文摘Perfluoroalkyl acids(PFAAs)are emerging persistent organic pollutants that are globally distributed in the environment.In the present review,the occurrence of PFAAs and their behavior in aquatic ecosystem were summarized,and the health and ecological risk assessment and the multimedia fate simulation were investigated.PFAAs are most likely to exist in the aqueous phase,and PFAAs in atmosphere are also able to enter water bodies through diffusion and wet and dry deposition and eventually become widely distributed in various environmental media.The air-solid partition is considered to be one of the major factors in the long-distance transportation of the pollutants.The pKa values and organic carbon fraction of the sediment could influence the partition of PFAAs between water and sediment.Otherwise,PFAAs have teratogenic,mutagenic and other toxic effects and they could be accumulated by biota,and magnified through trophic level.The ecological and health risks of PFOA and PFOS were assessment.In order to explore the partition mechanism and reduce the uncertainty of the simulation of the transport,transformation and fate,the experimental methods on physicochemical properties of PFAAs should be developed.Moreover,further studies on toxicities of PFAAs are necessary for health and ecological risk assessment. | Wenxiu Liu Jingyi Wu Wei He Fuliu Xu | 2019 | Ecosystem Health and Sustainability2019，5，1： | 3 |
| 15 | A novel Z-scheme CeO_(2)/g-C_(3)N_(4)heterojunction photocatalyst for degradation of Bisphenol A and hydrogen evolution and insight of the photocatalysis mechanism显示文摘CeO_(2)/g-C_(3)N_(4)heterojunction photocatalyst had been successfully fabricated through a one-step in-situ pyrolysis formation of 3D hollow CeO_(2)mesoporous nanospheres and 2D g-C_(3)N_(4)nanosheets together with simultaneous removal of carbon sphere templates after heat treatment.The sample shows high catalytic performances for photocatalytic hydrogen generation and photocatalytic oxidation of Bisphenol A(BPA)under visible light irradiation,and the catalytic degradation route of BPA was suggested by the degradation products determined by GC-MS.The enhancing catalytic activity was attributed to the effective interfacial charge migration and separation.Finally,it was proposed that the CeO_(2)/g-C_(3)N_(4)heterojunction photocatalyst could follow a more appropriate Z-scheme charge transfer mechanism,which was confirmed by the analysis of experiment and theoretical calculation results. | Wei Zhao Tiantian She Jingyi Zhang Guoxiang Wang Sujuan Zhang Wei Wei Gang Yang Lili Zhang Dehua Xia Zhipeng Cheng Haibao Huang Dennis Y.C.Leung | 2021 | Journal of Materials Science & Technology2021，，26： | 3 |
| 16 | Insulin resistance and the metabolism of branched-chain amino acids显示文摘Insulin resistance(IR)is a key pathological feature of metabolic syndrome and subsequently causes serious health problems with an increased risk of several common metabolic disorders.IR related metabolic disturbance is not restricted to carbohydrates but impacts global metabolic network.Branched-chain amino acids(BCAAs),namely valine,leucine and isoleucine,are among the nine essential amino acids,accounting for 35%of the essential amino acids in muscle proteins and 40%of the preformed amino acids required by mammals.The BCAAs are particularly responsive to the inhibitory insulin action on amino acid release by skeletal muscle and their metabolism is profoundly altered in insulin resistant conditions and/or insulin deficiency.Although increased circulating BCAA concentration in insulin resistant conditions has been noted for many years and BCAAs have been reported to be involved in the regulation of glucose homeostasis and body weight,it is only recently that BCAAs are found to be closely associated with IR.This review will focus on the recent findings on BCAAs from both epidemic and mechanistic studies. | Jingyi Lu Guoxiang Xie Weiping Jia Wei Jia | 2013 | Frontiers of Medicine2013，7，1： | 3 |
| 17 | The role of watermelon caffeic acid O-methyltransferase (ClCOMT1) in melatonin biosynthesis and abiotic stress tolerance显示文摘Melatonin is a pleiotropic signaling molecule that regulates plant growth and responses to various abiotic stresses.The last step of melatonin synthesis in plants can be catalyzed by caffeic acid O-methyltransferase(COMT),a multifunctional enzyme reported to have N-acetylserotonin O-methyltransferase(ASMT)activity;however,the ASMT activity of COMT has not yet been characterized in nonmodel plants such as watermelon(Citrullus lanatus).Here,a total of 16 putative O-methyltransferase(ClOMT)genes were identified in watermelon.Among them,ClOMT03(Cla97C07G144540)was considered a potential COMT gene(renamed ClCOMT1)based on its high identities(60.00–74.93%)to known COMT genes involved in melatonin biosynthesis,expression in almost all tissues,and upregulation under abiotic stresses.The ClCOMT1 protein was localized in the cytoplasm.Overexpression of ClCOMT1 significantly increased melatonin contents,while ClCOMT1 knockout using the CRISPR/Cas-9 system decreased melatonin contents in watermelon calli.These results suggest that ClCOMT1 plays an essential role in melatonin biosynthesis in watermelon.In addition,ClCOMT1 expression in watermelon was upregulated by cold,drought,and salt stress,accompanied by increases in melatonin contents.Overexpression of ClCOMT1 enhanced transgenic Arabidopsis tolerance against such abiotic stresses,indicating that ClCOMT1 is a positive regulator of plant tolerance to abiotic stresses. | Jingjing Chang Yanliang Guo Jingyi Yan Zixing Zhang Li Yuan Chunhua Wei Yong Zhang Jianxiang Ma Jianqiang Yang Xian Zhang Hao Li | 2021 | Horticulture Research2021，8，1： | 2 |
| 18 | Rapid isolation and immune profiling of SARS-CoV-2 specific memory B cell in convalescent COVID-19 patients via LIBRA-seq显示文摘B cell response plays a critical role against SARS-CoV-2 infection.However,little is known about the diversity and frequency of the paired SARS-CoV-2 antigen-specific BCR repertoire after SARS-CoV-2 infection.Here,we performed single-cell RNA sequencing and VDJ sequencing using the memory and plasma B cells isolated from five convalescent COVID-19 patients,and analyzed the spectrum and transcriptional heterogeneity of antibody immune responses.Via linking BCR to antigen specificity through sequencing(LIBRA-seq),we identified a distinct activated memory B cell subgroup(CD11c^(high) CD95^(high))had a higher proportion of SARS-CoV-2 antigen-labeled cells compared with memory B cells.Our results revealed the diversity of paired BCR repertoire and the non-stochastic pairing of SARS-CoV-2 antigen-specific immunoglobulin heavy and light chains after SARS-CoV-2 infection.The public antibody clonotypes were shared by distinct convalescent individuals.Moreover,several antibodies isolated by LIBRA-seq showed high binding affinity against SARS-CoV-2 receptor-binding domain(RBD)or nucleoprotein(NP)via ELISA assay.Two RBD-reactive antibodies C14646P3S and C2767P3S isolated by LIBRA-seq exhibited high neutralizing activities against both pseudotyped and authentic SARS-CoV-2 viruses in vitro.Our study provides fundamental insights into B cell response following SARS-CoV-2 infection at the single-cell level. | Bing He Shuning Liu Yuanyuan Wang Mengxin Xu Wei Cai Jia Liu Wendi Bai Shupei Ye Yong Ma Hengrui Hu Huicui Meng Tao Sun Yanling Li Huanle Luo Mang Shi Xiangjun Du Wenjing Zhao Shoudeng Chen Jingyi Yang Haipeng Zhu Yusheng Jie Yuedong Yang Deyin Guo Qiao Wang Yuwen Liu Huimin Yan Manli Wang Yao-Qing Chen | 2021 | Signal Transduction and Targeted Therapy2021，6，6： | 2 |
| 19 | Relationship between time in range and corneal nerve fiber loss in asymptomatic patients with type 2 diabetes显示文摘Background: Corneal confocal microscopy (CCM) is a noninvasive technique to detect early nerve damage of diabetic sensorimotor polyneuropathy (DSPN). Time in range (TIR) is an emerging metric of glycemic control which was reported to be associated with diabetic complications. We sought to explore the relationship between TIR and corneal nerve parameters in asymptomatic patients with type 2 diabetes (T2DM).Methods: In this cross-sectional study, 206 asymptomatic inpatients with T2DM were recruited. After 7 days of continuous glucose monitoring, the TIR was calculated as the percentage of time in the glucose range of 3.9 to 10.0 mmol/L. CCM was performed to determine corneal nerve fiber density, corneal nerve branch density, and corneal nerve fiber length (CNFL). Abnormal CNFL was defined as ≤15.30 mm/mm2.Results: Abnormal CNFL was found in 30.6% (63/206) of asymptomatic subjects. Linear regression analyses revealed that TIR was positively correlated with CCM parameters both in the crude and adjusted models (all P< 0.05). Each 10% increase in TIR was associated with a 28.2% (95% CI: 0.595–0.866,P = 0.001) decreased risk of abnormal CNFL after adjusting for covariates. With the increase of TIR quartiles, corneal nerve fiber parameters increased significantly (allP for trend <0.01). The receiver operating characteristic curve indicated that the optimal cutoff point of TIR was 77.5% for predicting abnormal CNFL in asymptomatic patients.Conclusions: There is a significant independent correlation between TIR and corneal nerve fiber loss in asymptomatic T2DM patients. TIR may be a useful surrogate marker for early diagnosis of DSPN. | Wejing Zhao Jingyi Lu Lei Zhang Wei Lu Wei Zhu Yuqian Bao Jjian Zhou | 2022 | Chinese Medical Journal2022，135，16： | 2 |
| 20 | Study on Lithium Insertion in Lepidocrocite and ,λ-MnO2 Type TiO2: A First-Principles Prediction显示文摘TiO2 是为可充电的锂电池的潜伏的阳极材料。我们的模拟当模特儿,基于纤铁矿和 -MnO2 类型 TiO2,被密度调查功能的理论(DFT ) 。关键问题集中于锂插入地点,电子结构,和 Li+ 离子的进行的路径。我们的计算数据显示 -MnO2 类型 TiO2 的计算电压比纤铁矿类型 TiO2 的高。纤铁矿类型 TiO2 沿着的 Li+ 离子迁居精力障碍[1 0 0 ] 方向(0.45 eV ) 比的低沿着[1 1 0 ] 方向(0.57 eV ) 。-MnO2 的精力障碍打 TiO2 在邻近的嵌入的地点(16c 或 8a 地点) 之中移动一个 Li+ 离子是 0.68 eV。 | Yahui Liu Jingyi Wu Wei Zhao Jinglong Chu Tao Qi | 2013 | Chinese Journal of Chemistry2013，31，10： | 2 |
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