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| 1 | Apoptosis and Proinflammatory Cytokine Responses of Primary Mouse Microglia and Astrocytes Induced by Human H1N1 and Avian H5N1 Influenza Viruses显示文摘Patients with an influenza virus infection can be complicated by acute encephalopathy and encephalitis. To investigate the immune reactions involved in the neurocomplication,mouse microglia and astrocytes were isolated,infected with human H1N1 and avian H5N1 influenza viruses,and examined for their immune responses. We observed homogeneously distributed viral receptors,sialic acid (SA)-α2,3-Galactose (Gal) and SA-α2,6-Gal,on microglia and astrocytes. Both viruses were replicative and productive in microglia and astrocytes. Virus-induced apoptosis and cytopathy in infected cells were observed at 24 h post-infection (p.i.). Expression of IL-1β,IL-6 and TNF-α mRNA examined at 6 h and 24 h p.i. was up-regulated,and their expression levels were considerably higher in H5N1 infection. The amounts of secreted proinflammatory IL-1β,IL-6 and TNF-α at 6 h and 24 h p.i. were also induced,with greater induction by H5N1 infection. This study is the first demonstration that both human H1N1 and avian H5N1 influenza viruses can infect mouse microglia and astrocytes and induce apoptosis,cytopathy,and proinflammatory cytokine production in them in vitro. Our results suggest that the direct cellular damage and the consequences of immunopathological injury in the CNS contribute to the influenza viral pathogenesis. | Gefei Wang Juan Zhang Weizhong Li Yun Su Yuanli Gao Heng Zhang Guimei Lin Xiaoyang Jiao Kangsheng Li | 2008 | Cellular & Molecular Immunology2008，5，2： | 28 |
| 2 | Microfluidic Electrospray Niacin Metal-Organic Frameworks Encapsulated Microcapsules for Wound Healing显示文摘Niacin metal-organic frameworks(MOFs)encapsulated microcapsules with alginate shells and copper-/zinc-niacin framework cores were in situ synthesized by using a microfuidic electrospray approach for wound healing.As the alginate shells were bacteriaresponsively degradable,the niacin MOFs encapsulated microcapsules could intelligently,controllably,and programmably release calcium,copper,and zinc ions,depending on the degree of infections.Te released ions could not only kill microbes by destroying their membrane and inducing the outfow of nutrient substance,but also activate copper/zinc superoxide dismutase(Cu/ZnSOD)to eliminate oxygen free radicals and rescue the cells from oxidative stress injury.Furthermore,the simultaneously released niacin could promote hemangiectasis and absorption of functional metal ions.Tus,the niacin MOFs encapsulated microcapsules were imparted with outstanding antibacterial,antioxidant,and angiogenesis properties.Based on an in vivo study,we have also demonstrated that the chronic wound healing process of an infected full-thickness skin defect model could be signifcantly enhanced by using the niacin MOFs encapsulated microcapsules as therapeutic agent.Terefore,the microfuidic electrospray niacin MOFs encapsulated microcapsules are potential for clinical applications. | Guopu Chen Yunru Yu Xiuwen Wu Gefei Wang Guosheng Gu Feng Wang Jianan Ren Huidan Zhang Yuanjin Zhao | 2019 | Research2019，，1： | 21 |
| 3 | Species-specific traits determine shrub-annual interactions during a growing season显示文摘Stress gradient hypothesis predicted that facilitative interactions usually increase in intensity and are importance with abiotic stress.By contrast,facilitation may be lost in time,when it involves the growth of benefactors or beneficiaries.Less is known about which response pattern is more common in arid desert.We present an empirical study to explore shrub-annual interactions at the community and individual level along the course of a single growing season in a desert steppe in northwest China.Here the severity of drought stress may increase in time due to uneven precipitation during plant growing season.We assessed growth responses of annuals in understory where two dominant shrubs were removed.Annuals responses showed a switch from weakly positive to more strongly positive beneath Calligonum mongolicum,whereas from positive to negative beneath Nitraria sphaerocarpa during the growing season.Additionally,annual species with contrasting functional traits showed distinct growth responses to canopies removal.There was evidence of an increase in soil moisture below the canopy of shrubs,but a decrease in potential evaporation rate and photosynthetically active radiation,which can partly explain these species-specific responses.We conclude that the balance between competitive and facilitative effects in shrub-annual interactions is not only governed by the severity of stress but also determined by plant traits,such as canopy structure of shrubs and functional traits of their understory annuals. | GeFei ZHANG WenZhi ZHAO | 2015 | Journal of Arid Land2015，7，3： | 4 |
| 4 | Effects of NS1 variants of H5N1 influenza virus on interferon induction, TNFa response and p53 activity显示文摘Non-structural protein 1(NS1)is an important virulence factor of the highly pathogenic H5N1 avian influenza virus.A five-amino-acid(5 aa)deletion at position 80–84 and an aspartic acid to glutamic acid substitution at position 92(D92E)are two major NS1 mutations that are highly correlated with enhanced virulence.To investigate the effect of these mutations in H5N1 virulence,three H5N1-NS1 variants were constructed:NS51(lacking 5 aa at position 80–84),NS51(I)(carrying a 5-aa insertion at position 80–84)and NS51(IM)(carrying both the 5-aa insertion and the D92E mutation).We examined the effects of these mutations on interferon(IFN)induction,tumor-necrosis factor(TNF)a response,p53 activity and apoptosis.We found that the D92E mutation eliminated NS1’s repressive effect on IFN induction,while the 5-aa deletion resulted in enhanced resistance to TNFa responses.We also observed that all three variants exhibited a similar suppressive effect on p53 transcriptional activity,although none of them significantly influenced apoptosis of host cells.Our findings shed new light on the role of NS1 in the pathogenicity of H5N1 virus. | Weizhong Li Gefei Wang Heng Zhang Gang Xin Dangui Zhang Jun Zeng Xiaoxuan Chen Yanxuan Xu Youhong Cui Kangsheng Li | 2010 | Cellular & Molecular Immunology2010，7，3： | 3 |
| 5 | Integration of high-performance spin-orbit torque MRAM devices by 200-mm-wafer manufacturing platform显示文摘We demonstrate in-plane field-free-switching spin-orbit torque(SOT)magnetic tunnel junction(MTJ)devices that are capable of low switching current density,fast speed,high reliability,and,most importantly,manufactured uniformly by the 200-mm-wafer platform.The performance of the devices is systematically studied,including their magnetic properties,switch-ing behaviors,endurance and data retention.The successful integration of SOT devices within the 200-mm-wafer manufactur-ing platform provides a feasible way to industrialize SOT MRAMs.It is expected to obtain excellent performance of the devices by further optimizing the MTJ film stacks and the corresponding fabrication processes in the future. | Hongchao Zhang Xiangyue Ma Chuanpeng Jiang Jialiang Yin Shuqin Lyu Shiyang Lu Xiantao Shang Bowen Man Cong Zhang Dandan Li Shuhui Li Wenjing Chen Hongxi Liu Gefei Wang Kaihua Cao Zhaohao Wang Weisheng Zhao | 2022 | Journal of Semiconductors2022，43，10： | 2 |
| 6 | Differential transcription-activating capability of NS1 proteins from different influenza virus subtypes expressed in yeast显示文摘Influenza A virus NS1 protein is an important regulatory factor with multiple functions and contributes greatly to viral pathogenesis.In the present study,transcription-activating potential of NS1 from different influenza A virus subtypes was examined in yeast two-hybrid system.The bait vectors contain-ing different NS1 genes,along with an empty prey vector,were transformed into yeast AH109(for growth assay on QDO plate and α-galactosidase assay),and Y187(for β-galactosidase assay).AH109 transformants with NS1 gene from H1N1,H5N1,and H9N2 viruses grew vigorously on the QDO plate and secreted high level of α-galactosidase.Also,Y187 bearing the above NS1 genes exhibited en-hanced β-galactosidase activity.Nevertheless,H3N2-NS1-transformed AH109 and Y187 yeasts did not grow on QDO plate and secrete β-galactosidase,respectively.These findings denote the remarkable variation in NS1 proteins from different influenza A virus subtypes on the transcription-stimulating capability in yeast. | LI WeiZhong,WANG GeFei,ZENG Jun,ZHANG DanGui,ZHANG Heng,CHEN XiaoXuan,CHEN YouYing & Li KangSheng Department of Microbiology and Immunology,Shantou University Medical College,Shantou 515041,China | 2009 | Science China(Life Sciences)2009，52，6： | 2 |
| 7 | In vivo antioxidant activity of polysaccharide fraction from Porphyra haita-nensis in aging mice显示文摘 | Zhang Quanbin Li Ning Zhang Gefei | 2003 | Pharmacol Res2003，48，2： | 1 |
| 8 | In vivo antioxidant activity of polysaccharide fraction from Porphyra haitanesis (Rhodephyta) in aging mice显示文摘 | ZHANG Quanbin LI Ning ZHOU Gefei | 2003 | Pharmacological Research2003，48，2： | 1 |
| 9 | In vivo antioxidant activity of polysaccharide fraction from Porphyra haitanesis (Rhodephyta) in aging mice显示文摘 | Quanbin Zhang Ning Li Gefei Zhou | 2003 | Pharmacological Research2003，48，2： | 1 |
| 10 | An Interactive platform for low-cost 3D building modeling from VGI data using convolutional neural network显示文摘The applications of 3D building models are limited as producing them requires massive labor and time costs as well as expensive devices.In this paper,we aim to propose a novel and web-based interactive platform,VGI3D,to overcome these challenges.The platform is designed to reconstruct 3D building models by using free images from internet users or volunteered geographic informa-tion(VGI)platform,even though not all these images are of high quality.Our interactive platform can effectively obtain each 3D building model from images in 30 seconds,with the help of user interaction module and convolutional neural network(CNN).The user interaction module provides the boundary of building facades for 3D building modeling.And this CNN can detect facade elements even though multiple architectural styles and complex scenes are within the images.Moreover,user interaction module is designed as simple as possible to make it easier to use for both of expert and non-expert users.Meanwhile,we conducted a usability testing and collected feedback from participants to better optimize platform and user experience.In general,the usage of VGI data reduces labor and device costs,and CNN simplifies the process of elements extraction in 3D building modeling.Hence,our proposed platform offers a promising solution to the 3D modeling community. | Hongchao Fan Gefei Kong Chaoquan Zhang | 2021 | Big Earth Data2021，5，1： | 1 |
| 11 | A Provably Secure General Construction for Key Exchange Protocols Using Smart Card and Password显示文摘Key exchange protocols using both smart card and password are widely used nowadays since they provide greater convenience and stronger security than protocols using only a password.Most of these protocols are often limited to simple network systems,and they may have security risks.We propose a general construction for key exchange protocols using smart card and password to avoid these flaws.The constructed protocol from the general construction has only one additional communication round than the original public encryption scheme.This construction is proven secure under random oracle model,so it can resist several common types of attacks.It is also adapted well to various networks.Compared with related protocols,the proposed key exchange protocol generated from the general construction has better secure properties and good computational efficiency in storage cost and operation time. | ZHANG Gefei FAN Dan ZHANG Yuqing LI Xiaowei | 2017 | Chinese Journal of Electronics2017，26，2： | 1 |
| 12 | In vivo antioxidant activity of polysaeeharide fraction from Porphyra haitanesis (Bhodephyta)in aging mice显示文摘 | Zhang Ouanbia Li Ning Gefei Zhou | 2003 | Pharmacological Research2003，48，2： | 1 |
| 13 | A Correlation Study on EFL Learning Anxiety and Oral Performance显示文摘The present study focuses on the correlation between English learning anxiety and students’oral English performance,and collects data through a questionnaire which is adapted from Horwitz et al’s Foreign Language Classroom Anxiety Scale(FLCAS).Students’oral test grades in TEM 4(Test for English Majors-Band 4)are used as a measurement of their oral proficiency.It is found that English-major students commonly have a moderate level of anxiety in their learning of English.Of the four dimensions in FLCAS,communication anxiety is the most intensive among most students,and test anxiety correlates most negatively with students’oral performance.The possible sources of English learning anxiety are explored and some suggestions are made accordingly after statistical analysis. | ZHANG Yao DONG Gefei | 2019 | US-China Foreign Language2019，17，6： | 0 |
| 14 | Phyllanthi Fructus:A modal medicinal and food homologous item in quality evaluation显示文摘Phyllanthi Fructus is a highly unique medicine and food homologous item,which exhibits distinctive flavor,notable nutritional value,and abundant pharmacological activity.It has enormous potential in the creation of health products and pharmaceuticals.However,due to the unique laws of quality formation and transfer of Phyllanthi Fructus,its appearance,shape,chemical compositions,nutrients,and sensory flavors are frequently greatly influenced by botanical resources,the processing and storage conditions.As a result,the current quality evaluation model is difficult to meet the needs of Phyllanthi Fructus as a medicine and food homologous item in the development of diversified products.This paper constructs the hierarchical utilization mode of Phyllanthi Fructus based on its unique quality formation and transmission laws,explores the quality evaluation model for food-oriented use and medicinal-oriented use,respectively,and systematically describes the quality evaluation idea under diversified application scenarios.This paper aims to serve as a reference for the construction of a quality evaluation model suitable for the medicine and food homologous item of Phyllanthi Fructus. | Gefei Li Yurou Jiang Dingkun Zhang Li Han Taigang Mo Sanhu Fan Haozhou Huang Junzhi Lin | 2023 | Chinese Herbal Medicines2023，15，3： | 0 |
| 15 | Heterologous interactions between NS1 proteins from different influenza A virus subtypes/strains显示文摘Non-structural protein 1(NS1) of the influenza virus plays a crucial role in modulating the host immune response and facilitating virus replication.The formation of a homodimer or an oligomer is necessary for NS1 to exert its function efficiently.In the present study,the NS1 protein from the A/Shantou/602/06(H3N2) virus(herein abbreviated as NS32) was found to interact with NS1 from A/Shantou/169/06(H1N1),A/Chicken/Guangdong/1/05(H5N1) and A/Quail/Hong Kong/G1/97(H9N2)(abbreviated as NS11,NS51 and NS92,respectively) viruses,although NS32 shares 17.4% 20.9% sequence diversity with NS11,NS51 and NS92.This indicates that the heterologous interactions between NS1 proteins from different influenza A virus subtypes/strains may be a common event during co-infection. | LI WeiZhong ZHANG Heng WANG GeFei ZHANG Chi ZENG XiangXing LIU Hui CHEN XiaoXuan XU YanXuan LI KangSheng | 2012 | Science China(Life Sciences)2012，55，6： | 0 |
| 16 | Spin-orbit torque efficiency enhancement to tungsten-based SOT-MTJs by interface modification with an ultrathin MgO显示文摘Spin-orbital torque (SOT) based three-terminal magnetic tunnel junctions (MTJs) have attracted much interest as the next generation magnetic random-access memory (MRAM)key device both in the academy and the industry.This is because SOT-MTJs feature high endurance and fast switching speed,benefiting tremendously from the separation of read/write current paths and less incubation time during the write in contrast to the spin-transfer torque (STT) MTJs [1]. | Shiyang LU Xiaobai NING Hongchao ZHANG Sixi ZHEN Xiaofei FAN Danrong XIONG Dapeng ZHU Gefei WANG Hong-Xi LIU Kaihua CAO Weisheng ZHAO | 2024 | Science China(Information Sciences)2024，67，1： | 0 |
| 17 | Bispecific antibodies in cancer therapy:Target selection and regulatory requirements显示文摘In recent years,the development of bispecific antibodies(bsAbs)has been rapid,with many new structures and target combinations being created.The boom in bsAbs has led to the successive issuance of industry guidance for their development in the US and China.However,there is a high degree of similarity in target selection,which could affect the development of diversity in bsAbs.This review presents a classification of various bsAbs for cancer therapy based on structure and target selection and examines the advantages of bsAbs over monoclonal antibodies(mAbs).Through database research,we have identified the preferences of available bsAbs combinations,suggesting rational target selection options and warning of potential wastage of medical resources.We have also compared the US and Chinese guidelines for bsAbs in order to provide a reference for their development. | Yanze Sun Xinmiao Yu Xiao Wang Kai Yuan Gefei Wang Lingrong Hu Guoyu Zhang Wenli Pei Liping Wang Chengliang Sun Peng Yang | 2023 | Acta Pharmaceutica Sinica B2023，13，9： | 0 |
| 18 | Demonstration of a manufacturable SOT-MRAM multiplexer array towards industrial applications显示文摘We have successfully demonstrated a 1 Kb spin-orbit torque(SOT)magnetic random-access memory(MRAM)multiplexer(MUX)array with remarkable performance.The 1 Kb MUX array exhibits an in-die function yield of over 99.6%.Additionally,it provides a sufficient readout window,with a TMR/RP_sigma%value of 21.4.Moreover,the SOT magnetic tunnel junctions(MTJs)in the array show write error rates as low as 10^(-6)without any ballooning effects or back-hopping behaviors,ensuring the write stability and reliability.This array achieves write operations in 20 ns and 1.2 V for an industrial-level temperature range from-40 to 125℃.Overall,the demonstrated array shows competitive specifications compared to the state-of-the-art works.Our work paves the way for the industrial-scale production of SOT-MRAM,moving this technology beyond R&D and towards widespread adoption. | Chuanpeng Jiang Jinhao Li Hongchao Zhang Shiyang Lu Pengbin Li Chao Wang Zhongkui Zhang Zhengyi Hou Xu Liu Jiagao Feng He Zhang Hui Jin Gefei Wang Hongxi Liu Kaihua Cao Zhaohao Wang Weisheng Zhao | 2023 | Journal of Semiconductors2023，44，12： | 0 |
| 19 | Novel phthalimides regulating PD-1/PD-L1 interaction as potential immunotherapy agents显示文摘Programmed cell death 1(PD-1)/programmed cell death ligand 1(PD-L1)have emerged as one of the most promising immune checkpoint targets for cancer immunotherapy.Despite the inherent advantages of small-molecule inhibitors over antibodies,the discovery of small-molecule inhibitors has fallen behind that of antibody drugs.Based on docking studies between small molecule inhibitor and PD-L1 protein,changing the chemical linker of inhibitor from a flexible chain to an aromatic ring may improve its binding capacity to PD-L1 protein,which was not reported before.A series of novel phthalimide derivatives from structure-based rational design was synthesized.P39 was identified as the best inhibitor with promising activity,which not only inhibited PD-1/PD-L1 interaction(IC_(50)=8.9 nmol/L),but also enhanced killing efficacy of immune cells on cancer cells.Co-crystal data demonstrated that P39 induced the dimerization of PD-L1 proteins,thereby blocking the binding of PD-1/PD-L1.Moreover,P39 exhibited a favorable safety profile with a LD_(50)>5000 mg/kg and showed significant in vivo antitumor activity through promoting CD8^(+)T cell activation.All these data suggest that P39 acts as a promising small chemical inhibitor against the PD-1/PD-L1 axis and has the potential to improve the immunotherapy efficacy of T-cells. | Chengliang Sun Yao Cheng Xiaojia Liu Gefei Wang Wenjian Min Xiao Wang Kai Yuan Yi Hou Jiaxing Li Haolin Zhang Haojie Dong Liping Wang Chenguang Lou Yanze Sun Xinmiao Yu Hongbin Deng Yibei Xiao Peng Yang | 2022 | Acta Pharmaceutica Sinica B2022，12，12： | 0 |
| 20 | Chiral structures of 6,12-dibromochrysene on Au(111)and Cu(111)surfaces显示文摘Nanoscale low-dimensional chiral architectures are increasingly receiving scientific interest,because of their potential applications in many fields such as chiral recognition,separation and transformation.Using 6,12-dibromochrysene(DBCh),we successfully constructed and characterized the large-area two-dimensional chiral networks on Au(111)and one-dimensional metal-liganded chiral chains on Cu(111)respectively.The reasons and processes of chiral transformation of chiral networks on Au(111)were analyzed.We used scanning tunneling spectroscopy(STS)to analyze the electronic state information of this chiral structure.This work combines scanning tunneling microscopy(STM)with non-contact atomic force microscopy(nc-AFM)techniques to achieve ultra-high-resolution characterization of chiral structures on low-dimensional surfaces,which may be applied to the bond analysis of functional nanofilms.Density functional theory(DFT)was used to simulate the adsorption behavior of the molecular and energy analysis in order to verify the experimental results. | Shijie Sun Baijin Li Boyu Fu Zilin Ruan Hui Zhang Wei Xiong Yong Zhang Gefei Niu Jianchen Lu Xiaoqing Zuo Lei Gao Jinming Cai | 2022 | Chinese Chemical Letters2022，33，12： | 0 |
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