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| 1 | A complete sequence and comparative analysis of a SARS-associated virus(Isolate BJ01)显示文摘The genome sequence of the Severe Acute Respiratory Syndrome (SARS)-associated virus provides essential information for the identification of pathogen(s), exploration of etiology and evolution, interpretation of transmission and pathogenesis, development of diagnostics, prevention by future vaccination, and treatment by developing new drugs. We report the complete genome sequence and comparative analysis of an isolate (BJ01) of the coronavirus that has been recognized as a pathogen for SARS. The genome is 29725 nt in size and has 11 ORFs (Open Reading Frames). It is composed of a stable region encoding an RNA-dependent RNA polymerase (composed of 2 ORFs) and a variable region representing 4 CDSs (coding sequences) for viral structural genes (the S, E, M, N proteins) and 5 PUPs (putative uncharacterized proteins). Its gene order is identical to that of other known coronaviruses. The sequence alignment with all known RNA viruses places this virus as a member in the family of Coronaviridae. Thirty putative substitutions have been identified by comparative analysis of the 5 SARS- associated virus genome sequences in GenBank. Fifteen of them lead to possible amino acid changes (non-synonymous mutations) in the proteins. Three amino acid changes, with predicted alteration of physical and chemical features, have been detected in the S protein that is postulated to beinvolved in the immunoreactions between the virus and its host. Two amino acid changes have been detected in the Mprotein, which could be related to viral envelope formation. Phylogenetic analysis suggests the possibility of non-human origin of the SARS-associated viruses but provides noevidence that they are man-made. Further efforts should focus on identifying the etiology of the SARS-associated virus and ruling out conclusively the existence of otherpossible SARS-related pathogen(s). | QIN E'de ZHU Qingyu YU Man FAN Baochang CHANG Guohui SI Bingyin YANG Bao PENG Wenming JIANG Tao LIU Bohua DENG Yongqiang LIU Hong ZHANG Yu WANG Cui LI Yuquan GAN Yonghua LI Xiaoyu L Fushuang TAN Gang CAO Wuchun, YANG Ruifu Institute of Microbiology and Epidemiology, Chinese Academy of Military Medical Sciences, Beijing 100071, China WANG Jian, LI Wei, XU Zuyuan, LI Yan, WU Qingfa, LIN Wei, CHEN Weijun, TANG Lin, DENG Yajun, HAN Yujun, LI Changfeng, LEI Meng, LI Guoqing, LI Wenjie, L Hong, SHI Jianping, TONG Zongzhong, ZHANG Feng, LI Songgang, LIU Bin, LIU Siqi, DONG Wei, WANG Jun, Gane K-S Wong, YU Jun & YANG Huanming* Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300 National Center for Genome Information, Beijing 101300, China | 2003 | Chinese Science Bulletin2003，48，10： | 121 |
| 2 | Whole-genome sequencing of 508 patients identifies key molecular features associated with poor prognosis in esophageal squamous cell carcinoma显示文摘Esophageal squamous cell carcinoma(ESCC)is a poor-prognosis cancer type with limited understanding of its molecular etiology.Using 508 ESCC genomes,we identified five novel significantly mutated genes and uncovered mutational signature clusters associated with metastasis and patients’outcomes.Several functional assays implicated that NFE2L2 may act as a tumor suppressor in ESCC and that mutations in NFE2L2 probably impaired its tumor-suppressive function,or even conferred oncogenic activities.Additionally,we found that the NFE2L2 mutations were significantly associated with worse prognosis of ESCC.We also identified potential noncoding driver mutations including hotspot mutations in the promoter region of SLC35E2 that were correlated with worse survival.Approximately 5.9%and 15.2%of patients had high tumor mutation burden or actionable mutations,respectively,and may benefit from immunotherapy or targeted therapies.We found clinically relevant coding and noncoding genomic alterations and revealed three major subtypes that robustly predicted patients’outcomes.Collectively,we report the largest dataset of genomic profiling of ESCC useful for developing ESCC-specific biomarkers for diagnosis and treatment. | Yongping Cui Hongyan Chen Ruibin Xi Heyang Cui Yahui Zhao Enwei Xu Ting Yan Xiaomei Lu Furong Huang Pengzhou Kong Yang Li Xiaolin Zhu Jiawei Wang Wenjie Zhu Jie Wang Yanchun Ma Yong Zhou Shiping Guo Ling Zhang Yiqian Liu Bin Wang Yanfeng Xi Ruifang Sun Xiao Yu Yuanfang Zhai Fang Wang Jian Yang Bin Yang Caixia Cheng Jing Liu Bin Song Hongyi Li Yi Wang Yingchun Zhang Xiaolong Cheng Qimin Zhan Yanhong Li Zhihua Liu-Show | 2020 | Cell Research2020，30，10： | 19 |
| 3 | Effect of filter-feeding fish silver carp on phytoplankton species and size distribution in surface water:A field study in water works显示文摘Silver carp were introduced into the pre-sedimentation pond to control excessive phytoplankton in raw water.The effectiveness of the filter-feeding silver carp on phytoplankton control and the effect of silver carp on phytoplankton community were investigated.The results showed that Microcystis could be effectively removed by silver carp stocked in the pre-sedimentation pond,and simultaneously,the concentration of single-cell phytoplankton increased obviously.The difference in phytoplankton species and singlecell phytoplankton size between in the water and in the gut of silver carp indicated that phytoplankton smaller than 5 μm,such as Chamydomonas and Platymonas,were almost not be filtered by silver carp,phytoplankton with the size between 5 and 20 μm could be partly filtered,and large size phytoplankton,mainly colony-forming Microcystis could be filtered almost completely.These filterfeeding characteristics directly caused the phytoplankton size distribution biased toward miniaturization.Therefore,this biological treatment using silver carp could be applied only to deal with groups of Microcystis-dominated eutrophic water,and was not appropriate in water bodies where single-cell micro phytoplankton were dominant.Especially when silver carp are used in water treatment,a cautious attitude should be taken based on the evaluation of phytoplankton biomass and species structure features in raw water. | Hua Ma Fuyi Cui Zhiquan Liu Zhenqiang Fan Wenjie He Peijun Yin | 2010 | Journal of Environmental Sciences2010，22，2： | 9 |
| 4 | Preliminary assessing no-surgical treatment response in bronchogenic carcinoma with double-phase contrast material-enhanced computed tomography显示文摘评估双阶段的功效的目的在 preliminarily 在 bronchogenic 癌估计没有外科的治疗反应对比提高材料的计算断层摄影术(CT ) 。有在没有外科的治疗以后的 bronchogenic 癌的 52 个病人经历了双阶段的方法对比提高材料的计算断层摄影术。在非离子的对比材料经由赌注被管理以后,二螺线 CT 扫描分别地在 25 和 90 秒点被获得以由使用一个汽车注射者的 4 mL/s 的率的肘的静脉。每扫描上的 Precontrast 和柱子对比变细被记录,山峰高度是计算的。改进模式在在对比媒介的注射以后在 25 和 90 秒点获得的图象上被评估。结果 Precontrast 变细,在 25 和 90 秒点的柱子对比变细是 42.20 ± 7.43 胡, 57.35 ± 10.09 胡和 71.85 ± 12.45 胡分别地。在 precontrast 变细的统计上重要的差别都没在我们在治疗前在情况中被获得的旧学习(吝啬的 precontrast 变细 40.70 胡) 在学习和结果在我们的结果之间被发现(t = 1.455, P = 0.152 >
0.05 ) 。在没有外科的治疗以后的 bronchogenic 癌的山峰高度(29.46 ± 10.85 胡) 是比在在我们的旧学习获得的治疗前的 bronchogenic 癌的显著地低的(吝啬的山峰高度 35.79 胡;t = 4.206, P = 0.001 <
0.05 ) 。52 个盒子中的 32 个在 90 秒点显示出同类的改进。32 个盒子,与不同类的改进有 21, 7 与不同类的改进, 2 与中央改进并且 2 与在 25 秒点的外部改进。在没有外科的处理以后的结论 Bronchogenic 癌在对比材料的管理以后显示出渐渐的增加到山峰高度。山峰高度能反映 bronchogenic 癌的血供应并且可能是为在 bronchogenic 癌的没有外科的治疗反应的评估的索引。 | Shenjiang Li Yong Zhao Yan Zhu Feng Zhu Debin Liu Wenjie Liang Xuefeng Cui Wenjie Bi | 2009 | The Chinese-German Journal of Clinical Oncology2009，8，3： | 9 |
| 5 | Bearing behavior and failure mechanism of squeezed branch piles显示文摘The current practice for the design of squeezed branch piles is mainly based on the calculated bearing capacity of circular piles. Insufficient considerations of the load-transfer mechanism, branch effect and failure mechanism, as well as overreliance on pile load tests, have led to conservative designs and limited application. This study performs full-scale field load tests on instrumented squeezed branch piles and shows that the shaft force curves have obvious drop steps at the branch position, indicating that the branches can effectively share the pile top load. The effects of branch position, spacing, number and diameter on the pile bearing capacity are analyzed numerically. The numerical results indicate that the squeezed branch piles have two types of failure mechanisms, i.e. individual branch failure mechanism and cylindrical failure mechanism. Further research should focus on the development of the calculation method to determine the bearing capacities of squeezed branch piles considering these two failure mechanisms. | Minxia Zhang Ping Xu Wenjie Cui Youbin Gao | 2018 | Journal of Rock Mechanics and Geotechnical Engineering2018，10，5： | 9 |
| 6 | Three-dimensional dissipative stress space considering yield behavior in deviatoric plane显示文摘Naturally deposited soils are always found in the complex three-dimensional stress state.Constitutive models developed for modeling the three-dimensional mechanical behavior of soils should obey the basic laws of thermo-mechanical principles.Based on the incremental dissipation function,a new deviatoric shift stress is derived and then introduced into the existing constitutive models to describe the yield behavior in the deviatoric plane for geomaterials.By adopting the proposed shift stress,the relationship between dissipative stress tensors and true stress tensors can be established.Therefore,the threedimensional plastic strain can be calculated reasonably through the associated flow rule in the three-dimensional dissipative stress space.At the same time,three methods that are conventionally adopted for generalizing constitutive models to model the three-dimensional stress-strain relationships are examined under the thermo-mechanical framework.The TS(transformed stress)method is shown to obey the thermo-mechanical rules and the TS space adopted in TS method is actually a translational three-dimensional dissipative stress space.However,it is illustrated that the other two approaches,the method of using failure criterion directly and the method of using g()function,violate the basic rules of thermo-mechanical theories although they may bring convenience and simplicity to numerical analysis for geotechnical engineering.Comparison between model predictions and experimental data confirms the validity of the proposed three-dimensional dissipative stress space. | YAO YangPing CUI WenJie WANG NaiDong | 2013 | Science China(Technological Sciences)2013，56，8： | 4 |
| 7 | Optimal power allocation for cognitive relay networks: amplify-and-forward versus selection relay显示文摘We consider a cognitive relay network which is defined by a source,a destination,and cognitive relay nodes and primary user nodes.In this network,a source is assisted by cognitive relay nodes which allow coexisting with primary user nodes by imposing severe constraints on the transmission power so that they operate below the noise floor of primary user nodes.In this paper,we mainly study the power allocation strategies of this system to minimize the outage probability subject to total and individual power constraints for cognitive relay nodes and subject to interference constraints for primary user nodes.A relay transmission scheme,namely amplify-and-forward (AF),is considered.We first present an optimal power allocation (OPA) scheme to minimize the system outage probability,based on instantaneous channel state information among the source,destination and relay nodes and mean channel gains between primary user nodes and cognitive relays.Next,we propose a selection AF scheme (S-AF) where the single best relay is chosen to assist in the transmission and study power allocation strategy for S-AF to minimize outage probability.The analytical power allocation strategies have been validated through numerical simulations.The results indicate that the AF with OPA and S-AF with OPA have significantly better outage behavior and average throughput than AF and S-AF schemes respectively.We also find that S-AF with OPA achieves a higher throughput,and hence lower outage probability than AF with OPA. | YUE WenJing ZHENG BaoYu MENG QingMin YUE WenJie CUI JingWu | 2011 | Science China(Information Sciences)2011，54，4： | 3 |
| 8 | 显示恶性孤立肺结节分叶征的理想CT层厚(英文)显示文摘Objective: The aim of this study was to determine an optimal slice thickness that was efficient in revealing lobulation of malignant solitary pulmonary nodules (SPNs) on multi-slice spiral computed tomography (MSCT) images preliminarily. Methods: Fifty patients with malignant SPNs (diameter ≤ 3 cm) underwent multidetector-row computed tomography of the chest in a single-breath-hold technique. The raw data were acquired with a collimation of 0.625 mm. Three sets of contiguous images were reconstructed with 1-, 2-, and 5-mm slice thickness, respectively. The lobulation sign of SPNs on the computed tomography (CT) images presented in 1-, 2-, and 5-mm slice thickness was compared. Using the 1-mm sections as the gold standard, an optimal slice thickness in revealing lobulation sign of SPNs was determined. Results: The 1-mm-thick images CT revealed 98 lobulations (25 with chord distance < 1 mm; 30 with chord distance 1-2 mm; 43 with chord distance > 2 mm) of 45 malignant SPNs. 18 lobulations with chord distance < 1 mm presented in 2-mm-thick sections were as same as those in 1-mm-thick sections. Statistically significant difference in lobulations number was found between that revealed in 2-mm-thick images and that in 1-mm-thick images (P = 0.023 < 0.05). 16 lobulations with chord distance < 1 mm presented in 5-mm-thick sections were as same as that in 1-mm-thick sections. There was statistically significant difference in lobulations number between that revealed in 5-mm-thick images and that in 1-mm-thick images (P = 0.004 < 0.05). The 24 lobulations with chord distance 1-2 mm presented in 2-mm-thick sections were as same as that in 1-mm-thick sections. No statistically significant difference in lobulations number were found between that revealed in 2-mm-thick images and that in 1-mm-thick images (P = 0.261 > 0.05). 13 lobulations with chord distance 1-2 mm presented in 5-mm-thick sections were as same as that in 1-mm-thick sections. There was statistically significant difference in lobulations number between that revealed in 5-mm-thick images and that in 1-mm-thick images (P = 0.003 < 0.05). 40 lobulations with chord distance ﹥2 mm presented in 2-mm-thick sections were as same as that in 1-mm-thick sections. No statistically significant difference in lobulations number was found between that revealed in 2-mm-thick images and that in 1-mm-thick images (P = 0.631 > 0.05). 36 lobulations with chord distance ﹥2 mm presented in 5-mm-thick sections were as same as that in 1-mm-thick sections. There was no statistically significant difference in lobulations number between that revealed in 5-mm-thick images and that in 1-mm-thick images (P = 0.264 > 0.05). Conclusion: It is suggested that the use of 1-mm slice thickness is suitable in revealing lobulations with chord distance < 1 mm. A 2-mm slice thickness is suggested to be used in revealing lobulations with chord distance 1-2 mm and 5-mm slice thickness to be used in revealing lobulations with chord distance > 2 mm. | Shenjiang Li Changcheng Li Xin Wang Debin Liu Wenjie Liang Feng Zhu Yan Zhu Xuefeng Cui Wenjie Bi | 2011 | The Chinese-German Journal of Clinical Oncology2011，10，10： | 2 |
| 9 | Homeobox B8 Targets Sterile Alpha Motif Domain-Containing Protein 9 and Drives Glioma Progression显示文摘Gliomas are the most commonly occurring tumors of the central nervous system. Glioblastoma multiforme (GBM) is the most malignant and aggressive brain cancer in adults. Further understanding of the mechanisms underlying the aggressive nature of GBM is urgently needed. Here we identified homeobox B8(HOXB8), a member of the homeobox family, as a crucial contributor to the aggressiveness of GBM. Data mining of publicly accessible RNA sequence datasets and our patient cohorts confirmed a higher expression of HOXB8 in the tumor tissue of GBM patients, and a strong positive correlation between the expression level and pathological grading of tumors and a negative correlation between the expression level and the overall survival rate. We next showed that HOXB8 promotes the proliferation and migration of glioblastoma cells and is crucial for the activation of the PI3K/AKT pathway and expression of epithelial–mesenchymal transition-related genes, possibly through direct binding to the promoter of SAMD9 (Sterile Alpha Motif Domain-Containing Protein 9) and activating its transcription. Collectively, we identified HOXB8 as a critical contributor to the aggressiveness of GBM, which provides insights into a potential therapeutic target for GBM and opens new avenues for improving its treatment outcome. | Wenping Ma Hongze Jin Wenjie Liu Xiaojuan Li Xingang Zhou Xinwu Guo Runfa Tian Qi Cui Junjie Luo Yueying Jiao Youtao Yu Haifeng Yang Hongshan Zhao | 2020 | Neuroscience Bulletin2020，36，4： | 2 |
| 10 | Tissue engineering of cartilage,tendon and bone显示文摘Tissue engineering aims to produce a functional tissue replacement to repair defects.Tissue reconstruction is an essential step toward the clinical application of engineered tissues.Significant progress has recently been achieved in this field.In our laboratory,we focus on construction of cartilage,tendon and bone.The purpose of this review was to summarize the advances in the engineering of these three tissues,particularly focusing on tissue regeneration and defect repair in our laboratory.In cartilage engineering,articular cartilage was reconstructed and defects were repaired in animal models.More sophisticated tissues,such as cartilage in the ear and trachea,were reconstructed both in vitro and in vivo with specific shapes and sizes.Engineered tendon was generated in vitro and in vivo in many animal models with tenocytes or dermal fibroblasts in combination with appropriate mechanical loading.Cranial and limb bone defects were also successfully regenerated and repaired in large animals.Based on sophisticated animal studies,several clinical trials of engineered bone have been launched with promising preliminary results,displaying the high potential for clinical application. | Hengyun SUN Wei LIU Guangdong ZHOU Wenjie ZHANG Lei CUI Yilin CAO | 2011 | Frontiers of Medicine2011，5，1： | 2 |
| 11 | Effects of Diet with 'Lianteng' Additive of Chinese Herbal Medicine on Blood Biochemical Indexes,Immune and Antioxidant Function of Guangxi Sanhuang Chickens显示文摘[Objectives]This trial was conducted to investigate the effects of diet with'Lianteng'additive of Chinese herbal medicine on blood biochemical indexes,immune and antioxidant function of Guangxi Sanhuang chickens.[Methods]480 Guangxi Sanhuang chickens at the age of 1 d were divided into 4 groups randomly and each group was assigned to 6 replicates with 20 chicks in each replicate;groups A,B and C were treatment groups and fed basal diets with 0.1%,0.2%and 0.4%of'Lianteng'additive of Chinese herbal medicine respectively during a trial of 70 d.Group D was control group and fed only basal diet.The serum samples were taken from 6 chicks in each group at the age of 21,35 and 49 d respectively and analyzed for biochemical,immune and antioxidant parameters in serum.[Results]Results from the trial showed that no difference was found in serum content of GLU,TG,TP,TC,and UN in the measurement at the age of 21,35 and 49 d(P>0.05);however,the content of ALB from groups A and B at 35 d was significantly higher than that from group D(P<0.01),and the ALB content from group B at 49 d was also higher than that from group D(P<0.05);the IgM content at 49 d from group A was lower than that from group D(P<0.05),in addition to this,there was no differences found in the contents of IgG,IgM and IFN-y among groups A,B,C and D during the trial(P>0.05);but the content of IL-2 from groups A and B at 49 d was significantly greater than that from group D(P<0.05);T-AOC from groups A,B and C at 49 d was very significantly higher than that from group D(P<0.01),and the one of group B at 35 d was still obviously higher than that of group D(P<0.05);also at 49 d,the GSH content in groups A and B was much greater than that in group D(P<0.01);T-SOD activity of group B and C at 35 d was distinctly greater than that of group D(P<0.05).[Conclusions]It can be concluded that the addition of 0.2%of'Lianteng'additive of Chinese herbal medicine in diet can increase immune function in a certain extent and promote the antioxidant ability observably for Guangxi Sanhuang chickens. | Sicong LUO Wenjie LU Lizhi LU Fulin WANG Qian QIN Cui YANG Jiahuang YANG | 2021 | Medicinal Plant2021，12，1： | 1 |
| 12 | Biomass-derived hard carbon microtubes with tunable apertures for high-performance sodium-ion batteries显示文摘Sodium-ion batteries(SIBs)are considered the most up-and-coming complements for large-scale energy storage devices due to the abundance and cheap sodium.However,due to the bigger radius,it is still a great challenge to develop anode materials with suitable space for the intercalation of sodium ions.Herein,we present hard carbon microtubes(HCTs)with tunable apertures derived from low-cost natural kapok fibers via a carbonization process for SIBs.The resulted HCTs feature with smaller surface area and shorter Na+diffusion path benefitting from their unique micro-nano structure.Most importantly,the wall thickness of HCTs could be regulated and controlled by the carbonization temperature.At a high temperature of 1,600℃,the carbonized HCTs possess the smallest wall thickness,which reduces the diffusion barrier of Na+and enhances the reversibility Na+storage.As a result,the 1600HCTs deliver a high initial Coulombic efficiency of 90%,good cycling stability(89.4%of capacity retention over 100 cycles at 100 mA·g^(−1)),and excellent rate capacity.This work not only charts a new path for preparing hard carbon materials with adequate ion channels and novel tubular micro-nano structures but also unravels the mechanism of hard carbon materials for sodium storage. | Pin Song Shiqiang Wei Jun Di Jun Du Wenjie Xu Daobin Liu Changda Wang Sicong Qiao Yuyang Cao Qilong Cui Pengjun Zhang Liaobo Ma Jiewu Cui Yan Wang Yujie Xiong | 2023 | Nano Research2023，16，4： | 1 |
| 13 | Elucidation and biological activities of a new polysaccharide from cultured Cordyceps militaris显示文摘 | Yongshuai Jing Xinlu Cui Zhiyan Chen Lijiao Huang Liyan Song Ting Liu Wenjie Lv Rongmin Yu | 2014 | Carbohydrate Polymers2014，，： | 1 |
| 14 | Complete Genome Sequences of the SARS-CoV: the BJ Group (Isolates BJ01-BJ04)显示文摘Beijing has been one of the epicenters attacked most severely by the SARS-CoV (severe acute respiratory syndrome-associated coronavirus) since the first patient was diagnosed in one of the city's hospitals. We now report complete genome sequences of the BJ Group, including four isolates (Isolates B J01, B J02, B J03,and B J04) of the SARS-CoV. It is remarkable that all members of the BJ Group share a common haplotype, consisting of seven loci that differentiate the group from other isolates published to date. Among 42 substitutions uniquely identifled from the BJ group, 32 are non-synonymous changes at the amino acid level.Rooted phylogenetic trees, proposed on the basis of haplotypes and other sequence variations of SARS-CoV isolates from Canada, USA, Singapore, and China, gave rise to different paradigms but positioned the BJ Group, together with the newly discovered GD01 (GD-Ins29) in the same clade, followed by the H-U Group (from Hong Kong to USA) and the H-T Group (from Hong Kong to Toronto), leaving the SP Group (Singapore) more distant. This result appears to suggest a possible transmission path from Guangdong to Beijing/Hong Kong, then to other countries and regions. | ShengliBi E‘deQin ZuyuanXu WeiLi JingWang YongWuHu YongLiu ShuminDuan JianfeiHu YujunHan JingXu YanLi YaoYi YongdongZhou WeiLin1 JieWen HongXu RuanLi ZizhangZhang HaiyanSun JinguiZhu ManYu BaochangFan QingfaWu WeiLin2 LinTang Bao’anYang GuoqingLi WenmingPeng WenjieLi TaoJiang YajunDeng BohuaLiu JianpingShi YongqiangDeng WeiWei HongLiu ZongzhongTong FengZhang YuZhang Cui‘eWang YuquanLi JiaYe YonghuaGan JiaJi XiaoyuLi XiangjunTian FushuangLu GangTan RuifuYang BinLiu SiqiLiu SonggangLi JunWang JianWang WuchunCao JunYu XiaopingDong HuanmingYang | 2003 | Genomics, Proteomics & Bioinformatics2003，1，3： | 1 |
| 15 | The transfection of embryonic stem cells with Tet-on system and its responsiveness to doxycycline显示文摘We transiently transfected pTet-on and pTRE2hyg-luciferase into the mouse em-bryonic stem cells (ESCs) using lipofectamine, and analyzed its inductive effect by adding serial concentrations of doxycycline (DOX). The results showed that in the transfected group, the luciferase activity of the cells was gradually increased along with the increasing concentration of DOX. While in the non-transfected group, the luciferase activity was not detectable even with DOX treatment. This indicated that the ESCs transfected with Tet-on system could response to DOX very well, and the regulation of target gene expression is dose dependent. | WANG Yan CONG Xiaoqian LIU Deli ZHANG Wenjie CUI Lei LIU Wei CAO Yilin | 2005 | Science China(Life Sciences)2005，48，4： | 1 |
| 16 | Organosilicon-group-derived silica-ionogel electrolyte for lithium ion batteries显示文摘In order to avoid leakage problem caused by liquid electrolyte, a new ionogel electrolyte was developed by in situ immobilizing organosilicon-functionalized ionic liquid within a nanoporous silica matrix. The ionic liquid evenly coats on the surface of porous silica and fills in the silica framework pores with no strong chemical interaction.The ionogel electrolyte has the dual advantages of a silica solid support and a wide electrochemical stability window of ionic liquid(4.87 V vs. Li^+/Li). The half-cells assembled with this electrolyte and LiFePO_4 electrode have excellent performance at room temperature and 60 ℃. The Li/SiO_2-IGE/LiFePO_4 cell displays a discharge capacity of 129.1 mAh·g^(-1) after 200 charge/discharge cycles at room temperature. | YueJiao Li Cui Guo LuShan Yue WenJie Qu Nan Chen YuJuan Dai RenJie Chen Feng Wu | 2018 | Rare Metals2018，37，6： | 1 |
| 17 | A rapidly magnetically assembled stem cell microtissue with“hamburger”architecture and enhanced vascularization capacity显示文摘With the development of magnetic manipulation technology based on magnetic nanoparticles(MNPs),scaffold-free microtissues can be constructed utilizing the magnetic attraction of MNP-labeled cells.The rapid in vitro construction and in vivo vascularization of microtissues with complex hierarchical architectures are of great importance to the viability and function of stem cell microtissues.Endothelial cells are indispensable for the formation of blood vessels and can be used in the prevascularization of engineered tissue constructs.Herein,safe and rapid magnetic labeling of cells was achieved by incubation with MNPs for 1 h,and ultrathick scaffold-free microtissues with different sophisticated architectures were rapidly assembled,layer by layer,in 5 min intervals.The in vivo transplantation results showed that in a stem cell microtissue with trisection architecture,the two separated human umbilical vein endothelial cell(HUVEC)layers would spontaneously extend to the stem cell layers and connect with each other to form a spatial network of functional blood vessels,which anastomosed with the host vasculature.The“hamburger”architecture of stem cell microtissues with separated HUVEC layers could promote vascularization and stem cell survival.This study will contribute to the construction and application of structural and functional tissues or organs in the future. | Yuezhi Lu Chun-Hua Yu Guangzheng Yang Ningjia Sun Fei Jiang Mingliang Zhou Xiaolin Wu Jiaxin Luo Cui Huang Wenjie Zhang Xinquan Jiang | 2021 | Bioactive Materials2021，6，11： | 1 |
| 18 | Viscosity study of dilute poly( acrylonitrile-ammonium itaconate) solutions 显示文摘 | Cui Chuansheng Wang Chengguo Jia Wenjie | 2006 | Journal of Polymer Research2006，13，： | 1 |
| 19 | Single cell transcriptomic analysis identifies novel vascular smooth muscle subsets under high hydrostatic pressure显示文摘Although some co-risk factors and hemodynamic alterations are involved in hypertension progression,their direct biomechanical effects are unclear.Here,we constructed a high-hydrostatic-pressure cell-culture system to imitate constant hypertension and identified novel molecular classifications of human aortic smooth muscle cells(HASMCs)by single-cell transcriptome analysis.Under 100-mmHg(analogous to healthy human blood pressure)or 200-mmHg(analogous to hypertension)hydrostatic pressure for 48 h,HASMCs showed six distinct vascular SMC(VSMC)clusters according to differential gene expression and gene ontology enrichment analysis.Especially,two novel HASMC subsets were identified,named the inflammatory subset,with CXCL2,CXCL3 and CCL2 as markers,and the endothelial-function inhibitory subset,with AKR1C2,AKR1C3,SERPINF1 as markers.The inflammatory subset promoted CXCL2&3 and CCL2 chemokine expression and secretion,triggering monocyte migration;the endothelial-function inhibitory subset secreted SERPINF1 and accelerated prostaglandin F2αgeneration to inhibit angiogenesis.The expression of the two VSMC subsets was greatly increased in arterial media from patients with hypertension and experimental animal models of hypertension.Collectively,we identified high hydrostatic pressure directly driving VSMCs into two new subsets,promoting or exacerbating endothelial dysfunction,thereby contributing to the pathogenesis of cardiovascular diseases. | Zhenzhen Chen Haizeng Zhang Yingnan Bai Changting Cui Shuangyue Li Wenjie Wang Yue Deng Qiannan Gao Lu Wang Wei Qi Lijun Zhang Yan Yang Bin Geng Jun Cai | 2021 | Science China(Life Sciences)2021，64，10： | 1 |
| 20 | Effects of Diet with'Lianhuang'Additive of Chinese Herb Medicine on Growth Performance and Metabolic Rates of Dietary Nutrients in Donglan Black Chicken显示文摘[Objectives]The study was conducted to evaluate the effects of diet with'Lianhuang'additive of Chinese herbal medicine(LACHM)on growth performance and metabolic rates of dietary nutrients in Donglan black chickens(DBCs).[Methods]80 DBCs aged 1 d were blocked into 4 groups randomly and assigned to 6 replicates in each group with 20 chicks in each replicate.Group A as control group fed basal diet.Groups B,C and D as treatment groups consumed the basal diet added with 0.25%,0.50%and 1.00%of LACHM,respectively during the test period of 70 d.In the end of feeding test,24 DBCs with similar body weight from each group were chosen out and used for a metabolism test to determine the metabolic rates of dietary nutrients from each treatment.The metabolism test was conducted as the same design as the feeding test and the DBCs from each group were fed the same diet as previous test.[Results]The ADG of chickens in the test increased by 5.00%-9.15%(P<0.05)except the stage of 50-70 d,and the individual average body weight(IABW)at 70 d in group B also increased by 4.86%(P<0.05).Compared with group A,the ADG of group C in the stages of 36-49,1-49 and 1-70 d and the IABW in the end of 70 d increased by 5.90%,2.66%,2.68%and 2.57%(P>0.05),respectively.The ADFI in the stage of 36-49 d from group A was 6.04%more than that from group B(P<0.01),and 5.12%and 4.13%more than those from groups C and D(P<0.05),respectively,and the ADFI of group A in the stage of 1-49 d was still 2.86%more than that from group C(P<0.05).However,no differences in ADFI were found in other stages in the test among the 4 groups(P>0.05).The F/G in the stages of 22-35 and 1-70 d from group B was 6.32%and 3.41%lower than those from group A(P<0.05),respectively,and even 11.43%and 7.31%lower in the stages of 36-49 and 1-49 d from group B than those from group A(P<0.01),respectively.The F/G of group C in the stage of 36-49 d was also 10.15%lower than that of group A(P<0.05),and still 5.29%lower than that from group A in 1-49 d(P<0.01),but no differences were found in other stages between the two groups(P>0.05).The metabolic rates of MD,CP,CF,NFE,CA,TE,Ca and P in the 3 treatment diets for group B,C and D all increased to some extent.Among them,the increasing extents of diets with 0.25%and 0.50%of LACHM for groups B and C were much greater.Therefore,the diet with 0.25%of LACHM could improve the growth performance and metabolic rates of dietary nutrients in DBCs significantly.[Conclusions]This study provides a theoretical reference for the effects of traditional Chinese medicine additives on the growth and metabolic rates of DBCs. | Jing YAN Lei QIU Min CHEN Fulin WANG Wenjie LU Menglei LI Cui YANG Jiahuang YANG | 2022 | Agricultural Biotechnology2022，11，3： | 1 |
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