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| 1 | Fast-Suppressor Screening for New Components in Protein Trafficking,Organelle Biogenesis and Silencing Pathway in Arabidopsis thaliana Using DEX-Inducible FREE1-RNAi Plants显示文摘Membrane trafficking is essential for plant growth and responses to external signals.The plant unique FYVE domain-containing protein FREE1 is a component of the ESCRT complex(endosomal sorting complex required for transport).FREE1 plays multiple roles in regulating protein trafficking and organelle biogenesis including the formation of intraluminal vesicles of multivesicular body(MVB),vacuolar protein transport and vacuole biogenesis,and autophagic degradation.FREE1 knockout plants show defective MVB formation,abnormal vacuolar transport,fragmented vacuoles,accumulated autophagosomes,and seedling lethality.To further uncover the underlying mechanisms of FREE1 function in plants,we performed a forward genetic screen for mutants that suppressed the seedling lethal phenotype of FREE1-RNAi transgenic plants.The obtained mutants are termed as suppressors of free1(sof).To date,229 putative sof mutants have been identified.Barely detecting of FREE1 protein with M3plants further identified 84 FREE1-related suppressors.Also145 mutants showing no reduction of FREE1 protein were termed as RNAi-related mutants.Through next-generation sequencing(NGS)of bulked DNA from F2mapping population of two RNAi-related sof mutants,FREE1-RNAi T-DNA inserted on chromosome 1 was identified and the causal mutation of putative sof mutant is being identified similarly.These FREE1-and RNAi-related sof mutants will be useful tools and resources for illustrating the underlying mechanisms of FREE1 function in intracellular trafficking and organelle biogenesis,as well as for uncovering the new components involved in the regulation of silencing pathways in plants. | Qiong Zhao Caiji Gao PoShing Lee Lin Liu Shaofang Li Tangjin Hu Jinbo Shen Shuying Pan Hao Ye Yunru Chen Wenhan Cao Yong Cui Peng Zeng Sheng Yu Yangbin Gao Liang Chen Beixin Mo Xin Liu Shi Xiao Yunde Zhao Silin Zhong Xuemei Chen Liwen Jiang | 2015 | Journal of Genetics and Genomics2015，42，6： | 4 |
| 2 | Increasing the efficiency of CRISPR/Cas9-based gene editing by suppressing RNAi in plants显示文摘Dear Editor,CRISPR (clustered regularly interspaced short palindromic repeats)/Cas9 (CRISPR-associated)-based genome editing is a powerful and widely adopted technology for introducing specific mutations in animal and plant systems. | Xiaoyan Wang Jiayun Lu Kangwen Lao Suikang Wang Xiaowei Mo Xintong Xu Xuemei Chen Beixin Mo | 2019 | Science China(Life Sciences)2019，62，7： | 4 |
| 3 | Uridylation and adenylation of RNAs显示文摘The posttranscriptional addition of nontemplated nucleotides to the 3′ ends of RNA molecules can have a significant impact on their stability and biological function. It has been recently discovered that nontemplated addition of uridine or adenosine to the 3′ ends of RNAs occurs in different organisms ranging from algae to humans, and on different kinds of RNAs, such as histone m RNAs, m RNA fragments, U6 sn RNA, mature small RNAs and their precursors etc. These modifications may lead to different outcomes, such as increasing RNA decay, promoting or inhibiting RNA processing, or changing RNA activity. Growing pieces of evidence have revealed that such modifications can be RNA sequence-specific and subjected to temporal or spatial regulation in development. RNA tailing and its outcomes have been associated with human diseases such as cancer. Here, we review recent developments in RNA uridylation and adenylation and discuss the future prospects in this research area. | SONG JianBo SONG Jun MO BeiXin CHEN XueMei | 2015 | Science China(Life Sciences)2015，58，11： | 3 |
| 4 | siRNA-mediated DNA methylation and H3K9 dimethylation in plants显示文摘Heterochromatic siRNAs regulate transcriptional gene silencing by inducing DNA methylation and histone H3K9 dimethylation.Recent advances have revealed the distinct phases involved in siRNA mediated silencing pathway,although the precise functions of a number of factors remain undesignated,putative mechanisms for the con-nection between DNA and histone methylation have been investigated,and much effort has been invested to understand the biological functions of siRNA-mediated epigenetic modifi cation.In this review,we summarize the mechanism of siRNA-mediated epigenetic modification,which involves the production of siRNA and the recruit-ments of DNA and histone methytransferases to the target sequences assisted by complementary pairing between 24-nt siRNAs and nascent scaffold RNAs,the roles of siRNA-mediated epigenetic modifi cation in maintaining genome stability and regulating gene expression have been dis-cussed,newly identified players of the siRNA mediated silencing pathway have also been introduced. | Chi Xu Jing Tian Beixin Mo | 2013 | Protein & Cell2013，4，9： | 2 |
| 5 | β-Mannosidase (EC 3.2.1.25) activity during and following germination of tomato (Lycopersicon esculentum Mill.) seeds. Purification, cloning and characterization显示文摘 | Beixin Mo Derek J. Bewley | 2002 | Planta2002，，1： | 1 |
| 6 | Protein arginine methyltransferase 3 fine-tunes the assembly/disassembly of pre-ribosomes to repress nucleolar stress by interacting with RPS2B in arabidopsis显示文摘Ribosome biogenesis,which takes place mainly in the nucleolus,involves coordinated expression of preribosomal RNAs(pre-rRNAs)and ribosomal proteins,pre-rRNA processing,and subunit assembly with the aid of numerous assembly factors.Our previous study showed that the Arabidopsis thaliana protein arginine methyltransferase AtPRMT3 regulates pre-rRNA processing;however,the underlying molecular mechanism remains unknown.Here,we report that AtPRMT3 interacts with Ribosomal Protein S2(RPS2),facilitating processing of the 90S/Small Subunit(SSU)processome and repressing nucleolar stress.We isolated an intragenic suppressor of atprmt3-2,which rescues the developmental defects of atprmt3-2 while produces a putative truncated AtPRMT3 protein bearing the entire N-terminus but lacking an intact enzymatic activity domain We further identified RPS2 as an interacting partner of AtPRMT3,and found that loss-of-function rps2a2b mutants were phenotypically reminiscent of atprmt3,showing pleiotropic developmental defects and aberrant pre-rRNA processing.RPS2B binds directly to pre-rRNAs in the nucleus,and such binding is enhanced in atprmt3-2.Consistently,multiple components of the 90S/SSU processome were more enriched by RPS2B in atprmt3-2,which accounts for early pre-rRNA processing defects and results in nucleolar stress.Collectively,our study uncovered a novel mechanism by which AtPRMT3 cooperates with RPS2B to facilitate the dynamic assembly/disassembly of the 90S/SSU processome during ribosome biogenesis and repress nucleolar stress. | Runlai Hang Zhen Wang Chao Yang Lilan Luo Beixin MO Xuemei Chen Jing Sun Chunyan Liu Xiaofeng Cao | 2021 | Molecular Plant2021，14，2： | 0 |
| 7 | NTP4 modulates miRNA accumulation via asymmetric modification of miRNA/miRNA duplex显示文摘Dear Editor.MicroRNAs(miRNAs)are endogenous small RNAs(sRNAs)that play crucial regulatory roles in gene expression.In plants,the transcribed primary miRNAs(pri-miRNAs)are sequentially processed by DICER-LIKE 1(DCL1)into precursor miRNAs(pre-miRNAs),and eventually miRNA/miRNA*duplexes(Wang et al.,2019).The methyltransferase HUA ENHANCER 1(HEN1)confers 2’-Omethylation on the 3’terminal ribose of each strand within the miRNA/miRNA*duplexes(Yu et al.,2005).Once methylated,miRNA duplexes are loaded onto ARGONAUTE1(AGO1),with one strand referred to as miRNA which is stabilized to form RNA-induced silencing complexes(RISCs),whereas the other strand becomes miRNA*that is eliminated and degraded. | Wenwen Kong Xianxin Dong Yongbing Ren Yuan Wang Xintong Xu Beixin Mo Yu Yu Xiaoyan Wang | 2021 | Science China(Life Sciences)2021，64，5： | 0 |
| 8 | A simple and efficient carbon nanotube-based nanocarriers simultaneously delivers multiple plasmids into diverse mature tissues of monocotyledonous crops显示文摘Dear Editor,With the development of high-throughput sequencing technology,a large number of functional genes have been identified.However,the illustration of these gene functions is limited,especially in monocot plants(Chen et al.,2022).One of the main reasons is that genetic transformation is still a major bottleneck for these plants.Recently,nanoparticles(NPs)-mediated biomolecular delivery methods. | Xuedong Liu Wenwen Kong Nian Xiao Lijuan Huang Yu Zhang Weizhao Chen Beixin Mo Yu Yu | 2023 | Science China(Life Sciences)2023，66，7： | 0 |
| 9 | Study on RNAi-based herbicide for Mikania micrantha显示文摘The invasive plant Mikania micrantha Kunth(M.micrantha)from South America poses a significant threat to the stability and biodiversity of ecosystems.However,an effective and economical method to control M.micrantha is still lacking.RNA interference(RNAi)has been widely studied and applied in agriculture for trait improvement.Spray-induced gene silencing(SIGS)can produce RNAi silencing effects without introducing heritable modifications to the plant genome and is becoming a novel nontransformation strategy for plant protection.In this study,the genes encoding chlorophyll a/b-binding proteins were selected as targets of RNAi,based on high-throughput sequencing of M.micrantha transcriptome and bioinformatic analyses of sequence specificity.Three types of RNAi molecules,double-stranded RNA,RNAi nanomicrosphere,and short hairpin RNA(shRNA),with their corresponding short interfering RNA sequences were designed and synthesized for SIGS vector construction,from which each RNAi molecule was transcribed and extracted to be sprayed on M.micrantha leaves.Whereas water-treated control leaves remained green,leaves treated with RNAi molecules turned yellow and eventually wilted.Quantitative real-time PCR showed that the expression levels of target genes were significantly reduced in the RNAi-treated groups compared with those of the control,suggesting that all three types of RNAi herbicides effectively silenced the endogenous target genes,which are essential for the growth of M.micrantha.We also found that shRNA showed better silencing efficiency than the other two molecules.Taken together,our study successfully designed three types of RNAi-based herbicides that specifically silenced endogenous target genes and controlled the growth of M.micrantha.Moreover,we identified a gene family encoding chlorophyll a/b-binding proteins that is important for the growth and development of M.micrantha and could serve as potential targets for controlling the spread of M.micrantha. | Jiantao Mai Lingling Liao Rongsong Ling Xiaolong Guo Jingying Lin Beixin Mo Weizhao Chen Yu Yu | 2021 | Synthetic and Systems Biotechnology2021，6，4： | 0 |
| 10 | NAD^(+)-capped RNAs are widespread in rice(Oryza sativa)and spatiotemporally modulated during development显示文摘Dear Editor,Nicotinamide adenine dinucleotide(NAD+),a nucleotidecontaining metabolite,can be incorporated as an RNA 5′cap to form NAD^(+)-capped RNAs(NAD-RNAs),which have been identified in bacteria,yeast,mammalian cells and Arabidopsis thaliana(Hu et al.,2021).Most NAD-RNAs are m RNAs encoded by the nuclear and mitochondrial genomes。 | Haijiao Dong Xufeng Wang Cong Tan Lei Gao Jie Cui Lin Liu Beixin Mo Yongzhong Xing Yu Yu Xuemei Chen | 2022 | Science China(Life Sciences)2022，65，10： | 0 |
| 11 | miR319-TCPs-TGA9/TGA10/ROXY2 regulatory module controls cell fate specification in early anther development in Arabidopsis显示文摘Dear Editor,The process of anther development is generally conserved across plant species(Ma,2005).Many protein-coding genes and noncoding RNAs have been identified to participate in early anther development in Arabidopsis(Walbot and Egger,2016).mi R319 is one of the most ancient and conserved mi RNA families in land plants(Chávez Montes et al.,2014). | Jinyuan Tao Zijie Pan Wenwen Kong Beixin Mo Xuemei Chen Yu Yu | 2024 | Science China(Life Sciences)2024，67，4： | 0 |
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