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| 1 | Microfluidic Electrospray Niacin Metal-Organic Frameworks Encapsulated Microcapsules for Wound Healing显示文摘Niacin metal-organic frameworks(MOFs)encapsulated microcapsules with alginate shells and copper-/zinc-niacin framework cores were in situ synthesized by using a microfuidic electrospray approach for wound healing.As the alginate shells were bacteriaresponsively degradable,the niacin MOFs encapsulated microcapsules could intelligently,controllably,and programmably release calcium,copper,and zinc ions,depending on the degree of infections.Te released ions could not only kill microbes by destroying their membrane and inducing the outfow of nutrient substance,but also activate copper/zinc superoxide dismutase(Cu/ZnSOD)to eliminate oxygen free radicals and rescue the cells from oxidative stress injury.Furthermore,the simultaneously released niacin could promote hemangiectasis and absorption of functional metal ions.Tus,the niacin MOFs encapsulated microcapsules were imparted with outstanding antibacterial,antioxidant,and angiogenesis properties.Based on an in vivo study,we have also demonstrated that the chronic wound healing process of an infected full-thickness skin defect model could be signifcantly enhanced by using the niacin MOFs encapsulated microcapsules as therapeutic agent.Terefore,the microfuidic electrospray niacin MOFs encapsulated microcapsules are potential for clinical applications. | Guopu Chen Yunru Yu Xiuwen Wu Gefei Wang Guosheng Gu Feng Wang Jianan Ren Huidan Zhang Yuanjin Zhao | 2019 | Research2019，，1： | 21 |
| 2 | Bioinspired Adhesive and Antibacterial Microneedles for Versatile Transdermal Drug Delivery显示文摘Microneedles have attracted increasing interest among various medical fields due to their painless,noninvasive,and efficient way of drug delivery.However,practical applications of these microneedles in different epidermal locations and environments are still restricted by their low adhesion and poor antimicrobial activity.Here,inspired by the antibacterial strategy of Paenibacillus polymyxa and adhesion mechanisms of mussel byssi and octopus tentacles,we develop hierarchical microneedles with multifunctional adhesive and antibacterial abilities.With polydopamine hydrogel as the microneedle base and a loop of suctioncup-structured concave chambers encircling each microneedle,the generated microneedles can fit the skin well;keep strong adhesion in dry,moist,and wet environments;and realize self-repair after being split into two parts.Besides,as polymyxin is loaded into both the hydrogel tips and the polydopamine base,the microneedles are endowed with excellent ability to resist common bacteria during storage and usage.We have demonstrated that these microneedles not only showed excellent adhesion when applied to knuckles and ideal antibacterial activity but also performed well in drug-sustained release and treatment for the osteoarthritis rat model.These results indicate that bioinspired multifunctional microneedles will break through the limitation of traditional methods and be ideal candidates for versatile transdermal drug delivery systems. | Xiaoxuan Zhang Guopu Chen Yunru Yu Lingyu Sun Yuanjin Zhao | 2020 | Research2020，，1： | 14 |
| 3 | Bio-inspired clamping microneedle arrays from flexible ferrofluid-configured moldings显示文摘Microneedle(MN)arrays have demonstrated value for cosmetics,diagnosis,transdermal drug delivery,and other biomedical areas.Much effort has been devoted to developing simple stratagem for creating versatile moldings and generating functional MN arrays.Here,inspired by the serrated microstructure of mantises’forelegs,we present a novel serration-like clamping MN array based on ferrofluidconfigured moldings.Benefiting from the flexibility and versatility of ferrofluids,negative microhole array moldings with various sizes and angles toward the midline could be created easily.The corresponding biocompatible polymer MN arrays with both isotropic and anisotropic structures could then be produced feasibly and cost-effectively by simply replicating these moldings.It was found that the resultant serrated clamping MN arrays had the ability to adhere to skin firmly,enabling them to be used over a relatively long time and while the recipient was moving.This proposed technology performed well in minimally invasive drug administration and sustained glucocorticoids release during treatment for imiquimod-induced psoriasis in mice.These features indicated that such MN arrays could play important roles in wearable transdermal drug delivery systems and in other applications. | Xiaoxuan Zhang Fengyuan Wang Yunru Yu Guopu Chen Luoran Shang Lingyu Sun Yuanjin Zhao | 2019 | Science Bulletin2019，64，15： | 11 |
| 4 | Fast-Suppressor Screening for New Components in Protein Trafficking,Organelle Biogenesis and Silencing Pathway in Arabidopsis thaliana Using DEX-Inducible FREE1-RNAi Plants显示文摘Membrane trafficking is essential for plant growth and responses to external signals.The plant unique FYVE domain-containing protein FREE1 is a component of the ESCRT complex(endosomal sorting complex required for transport).FREE1 plays multiple roles in regulating protein trafficking and organelle biogenesis including the formation of intraluminal vesicles of multivesicular body(MVB),vacuolar protein transport and vacuole biogenesis,and autophagic degradation.FREE1 knockout plants show defective MVB formation,abnormal vacuolar transport,fragmented vacuoles,accumulated autophagosomes,and seedling lethality.To further uncover the underlying mechanisms of FREE1 function in plants,we performed a forward genetic screen for mutants that suppressed the seedling lethal phenotype of FREE1-RNAi transgenic plants.The obtained mutants are termed as suppressors of free1(sof).To date,229 putative sof mutants have been identified.Barely detecting of FREE1 protein with M3plants further identified 84 FREE1-related suppressors.Also145 mutants showing no reduction of FREE1 protein were termed as RNAi-related mutants.Through next-generation sequencing(NGS)of bulked DNA from F2mapping population of two RNAi-related sof mutants,FREE1-RNAi T-DNA inserted on chromosome 1 was identified and the causal mutation of putative sof mutant is being identified similarly.These FREE1-and RNAi-related sof mutants will be useful tools and resources for illustrating the underlying mechanisms of FREE1 function in intracellular trafficking and organelle biogenesis,as well as for uncovering the new components involved in the regulation of silencing pathways in plants. | Qiong Zhao Caiji Gao PoShing Lee Lin Liu Shaofang Li Tangjin Hu Jinbo Shen Shuying Pan Hao Ye Yunru Chen Wenhan Cao Yong Cui Peng Zeng Sheng Yu Yangbin Gao Liang Chen Beixin Mo Xin Liu Shi Xiao Yunde Zhao Silin Zhong Xuemei Chen Liwen Jiang | 2015 | Journal of Genetics and Genomics2015，42，6： | 4 |
| 5 | The synthesis of ehitin-g-poly(vinyl acetate)copoly-mers with a redox initiator 显示文摘 | Don Trongming Chen Yunru Chu Wenyen | 2002 | Journal of Polymer Research2002，9，4： | 1 |
| 6 | Refractory humic-like dissolved organic matter fuels microbial communities in deep energy-limiting marine sediments显示文摘Humic-like dissolved organic matter(DOM),usually regarded as refractory,is a major component of DOM in marine sediment pore waters.However,its bio-reactivity remains poorly explored in natural environments,which makes its roles in supporting subsurface microbial communities and regulating long-term carbon cycling elusive.Here,the bio-reactivity of humiclike DOM was evaluated by modeled reaction rates together with its interactions with microbial communities in five sediment cores collected from the eutrophic Pearl River Estuary to the oligotrophic deep-sea basin in the northern South China Sea.We revealed contrasting relationships between humic-like DOM and microbes in the coastal and deep-sea sediments.In eutrophic coastal sediments,specific microbial groups enriched in the deep layers co-varied with humic-like DOM,while most microbial groups were significantly correlated with protein-like DOM,microbial transformation of which likely resulted in the production of humic-like DOM.On the contrary,in energy-limiting deep-sea sediments,over 70%of the microbial groups were found closely correlated with humic-like DOM,a net consumption of which was demonstrated in deep layers.The consumption of humic-like DOM in deep-sea sediments reduced its total production flux in the uppermost~5-meter layer to about one-tenth of that in coastal sediments,which could consequently decrease the refractory DOM flux to the overlying seawater and influence long-term oceanic carbon cycling. | Yunru CHEN Weikang SUI Jing WANG Ding HE Liang DONG Joanna JWANIEK Fengping WANG | 2023 | Science China Earth Sciences2023，66，8： | 1 |
| 7 | Hetero-bivalent nanobodies provide broad-spectrum protection against SARS-CoV-2 variants of concern including Omicron显示文摘SARS-CoV-2 variants with adaptive mutations have continued to emerge,causing fresh waves of infection even amongst vaccinated population.The development of broad-spectrum antivirals is thus urgently needed.We previously developed two hetero-bivalent nanobodies(Nbs),aRBD-2-5 and aRBD-2-7,with potent neutralization activity against the wild-type(WT)Wuhan isolated SARS-CoV-2,by fusing aRBD-2 with aRBD-5 and aRBD-7,respectively.Here,we resolved the crystal structures of these Nbs in complex with the receptor-binding domain(RBD)of the spike protein,and found that aRBD-2 contacts with highly conserved RBD residues and retains binding to the RBD of the Alpha,Beta,Gamma,Delta,Delta plus,Kappa,Lambda,Omicron BA.1,and BA.2 variants.In contrast,aRBD-5 and aRBD-7 bind to less conserved RBD epitopes non overlapping with the epitope of aRBD 2,and do not show apparent binding to the RBD of some variants.However,when fused with aRBD-2,they effectively enhance the overal binding affinity.Consistently,aRBD-2-5-Fc and aRBD-2-7 Fc potently neutralized all of the tested authentic or pseudotyped viruses,incuding WT,Alpha,Beta,Gamma,Delta,and Omicron BA.1,BA.1.1 and BA.2.Furthermore,aRBD-2-5-FC provided prophylactic protection against the WT and mouse-adapted SARS CoV-2 in mice,and conferred protection against the Omicron BA.1 variant in hamsters prophylatically and therapeutically,indicating that aRBD-2-5-Fc could potentially beneft the prevention and treatment of COVID-19 caused by the emerging variants of concern.Our strategy provides new solutions in the development of broad-spectrum therapeutic antibodies for COVID-19. | Huan Ma Xinghai Zhang Peiyi Zheng Peter H.Dube Weihong Zeng Shaohong Chen Qingyu Cheng Yunru Yang Yan Wu Junhui Zhou Xiaowen Hu Yan Xiang Huajun Zhang Sandra Chiu Tengchuan Jin | 2022 | Cell Research2022，32，9： | 1 |
| 8 | Single-dose AAV-based vaccine induces a high level of neutralizing antibodies against SARS-CoV-2 in rhesus macaques显示文摘Dear Editor,Coronavirus disease 2019(COVID-19)is a highly infectious respiratory disease that continues to pose a serious global public health emergency.The disease shows a high infection rate,long incubation period,and rapidly emerging variants,which have led to its rapid spread worldwide(Krammer 2020).Many vaccines have been developed for the control of severe acute respiratory syndrome coronavirus 2(SARS-CoV-2),the virus responsible for COVID-19,including vaccines based on messenger RNA(mRNA)(Polack et al.2020),viral vectors(Zhu et al.2020),recombinant proteins(Yang et al.2020),and inactivated SARS-CoV-2(Zhang et al.2021). | Dali Tong Mei Zhang Yunru Yang Han Xia Haiyang Tong Huajun Zhang Weihong Zeng Muziying Liu Yan Wu Huan Ma Xue Hu Weiyong Liu Yuan Cai Yanfeng Yao Yichuan Yao Kunpeng Liu Shifang Shan Yajuan Li Ge Gao Weiwei Guo Yun Peng Shaohong Chen Juhong Rao Jiaxuan Zhao Juan Min Qingjun Zhu Yanmin Zheng Lianxin Liu Chao Shan Kai Zhong Zilong Qiu Tengchuan Jin Sandra Chiu Zhiming Yuan Tian Xue | 2023 | Protein & Cell2023，14，1： | 0 |
| 9 | Cuticular compounds inhibit cannibalism of early-instar spiderlings by pulli-carrying Pardosa pseudoannulata females显示文摘Cannibalism is common in spiders.Wolf spider(Lycosidae)females,which exhibit extensive maternal care,have been reported to cannibalize less when they are carrying egg sacs and juveniles.In a laboratory experiment,we demonstrated that cannibalism of early-instar spiderlings(EIS)by a wolf spider(Pardosa pseudoannulata)mother was almost completely inhibited when she was carrying spiderlings.Compared with virgin and mated-females,mother spiders tolerated more and predated fewer spiderlings,including gregarious pulli and newly dispersed spiderlings(NDS).Cannibalism of EIS by females during their reproductive period exhibited a V-shaped pattern,with a gradual decrease from the egg sac-carrying to pulli-carrying(PC)stage,and a recovery from the PC stage to post-reproductive(PR)stage.Notably,there was 0 cannibalism at the PC stage.PC females exhibited no interest in pulli,while PR females were attracted to and predated pulli and NDS as they did their natural prey,Nilaparvata lugens.Interestingly,PC females captured and released NDS in a foraging assay,although attraction was observed from olfactometer measurements.PC mothers possessed a cuticular volatile profile that was closer to that of pulli and NDS than to that of PR females.Moreover,NDS cuticular extract provoked an electrophysiological response in legs of PC females.Therefore,cuticular compound-mediated chemical communication may be involved in inhibiting cannibalism of EIS by spider mothers,and especially in eliminating cannibalism by PC mothers.Future studies will aim to characterize the specific cuticular compounds and chemoreception mechanism in females,which will facilitate our understanding of intraspecific recognition and cannibalism in spiders. | Na Yu Yunru Chen Guangming Xu Zhiming Yang Shijie Wang Tianyu Lu Yixi Zhang Zewen Liu | 2022 | Insect Science2022，29，5： | 0 |
| 10 | The risk factors and threshold level of subchronic inhalation exposure of reclaimed water显示文摘Inhalation of reclaimed water is known to cause lung infammation,and free endotoxins have been shown to be a major risk factor for acute exposure.Subchronic exposure has also been shown to induce infammatory responses with visible tissue damage.However,subchronic risk factors have yet to be identified,and a threshold for the protection of occupational populations during urban reuse is necessary.In this study,potential risk factors in reclaimed water were examined by subchronic exposure with fractionated reclaimed water,and the health risk threshold was tested with a series of diluted reclaimed water.Accordingly,following a 12-week exposure,macromolecules and microorganisms were found to be two major risk factors in reclaimed water that could cause pulmonary infammation,including increased proportion of polymorphonuclear leukocytes in bronchoalveolar fuid,formation of inducible bronchus-associated lymphoid tissue,and elevation of Immunoglobulin A levels.Moreover,infammation persisted after a 4-week recovery period.The calculated threshold of reclaimed water exposure for mice was 31.8 Endotoxin Unit(EU)/(kg·day)under when exposed to 50%additional relative humidity from reclaimed water at 25℃ for 2 hr/day.Meanwhile,the subchronic threshold estimate for humans under the same exposure conditions was found to be 12.2 EU/(kg·day),corresponding to endotoxin levels of 61.7 EU/mL in reclaimed water.The threshold level of endotoxin was lower than that in most non-potable reclaimed water.The findings of this study suggest that occupational exposure of reclaimed water can serve as a potential risk to workers. | Jiayang Kong Yun Lu Yunru Ren Menghao Chen Gang Liu Liangliang Shi | 2024 | Journal of Environmental Sciences2024，，3： | 0 |
| 11 | Microtubes with gradient decellularized porcine sciatic nerve matrix from microfluidics for sciatic nerve regeneration显示文摘Long-range peripheral nerve defect is a severe and worldwide disease.With the increasing development of tissue engineering,the excellent ability of nerve extracellular matrix(ECM)in peripheral nerve injury(PNI)has been widely studied and verified.Here,we present a novel microtube that contains gradient decellularized porcine sciatic nerve ECM hydrogel(pDScNM-gel)from microfluidics for sciatic nerve regeneration.The pDScNM is confirmed to enhance cell proliferation and migration,and improve the axon growth of primary dorsal root ganglions(DRGs)in a concentration-related manner.These behaviors were also achieved when cells were co-cultured in a gradient pDScNM microtube.The in vivo sciatic nerve regeneration and functional recovery were also demonstrated by assembling the gradient pDScNM microtubes with a medical silicon tube.These results indicated that the microtubes with gradient pDScNM could act as a promising alternative for repairing peripheral nerve defects and showed great potential in clinical use. | Binghui Jin Yunru Yu Xiangxiang Chen Yanhong Yang Yushan Xiong Young Jun Im Yuanjin Zhao Jian Xiao | 2023 | Bioactive Materials2023，，3： | 0 |
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