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| 1 | 脱落酸调控种子休眠和萌发的分子机制显示文摘脱落酸(ABA)是调控种子休眠和萌发过程的主要植物激素。种子内源ABA含量和种胚对ABA敏感性共同调控种子休眠和萌发过程,确保植物种子以休眠状态在逆境中保持其自身繁衍能力,并在适宜的环境下启动萌发程序。种子ABA合成代谢和ABA信号转导途径涉及许多重要基因家族,它们通过复杂的调控网络精确地控制着种胚发生、种子成熟、休眠及萌发进程。该文对ABA调控种子休眠和萌发的分子机制最新研究进展进行综述,并展望了今后的研究方向。 | 伍静辉 谢楚萍 田长恩 周玉萍 | 2018 | 植物学报2018,53,4: | 21 |
| 2 | 光信号与激素调控种子休眠和萌发研究进展显示文摘休眠是种子植物在长期进化过程中产生的适应性性状,通过抑制种子在不适宜的环境中萌发进而保证植物能够在逆境中生存。此外,休眠有助于种子的长距离运输和扩散,因此休眠对种子延续和物种保存具有重要意义。种子由休眠向萌发的发育转变不仅关系到物种的繁衍,而且对保证农业生产中作物的产量和品质也具有重要作用。种子的休眠和萌发受到内源激素和外源光信号的共同调控。其中,外源光信号主要通过调控内源ABA和GA的生物合成及信号转导进而调控种子休眠和萌发。该文系统综述了外源光信号和内源激素调控种子休眠和萌发的作用通路以及两类信号通路之间的交互作用,旨在为农业生产中利用光和激素调控种子休眠与萌发提供参考。 | 杨立文 刘双荣 林荣呈 | 2019 | 植物学报2019,54,5: | 13 |
| 3 | The role of light in regulating seed dormancy and germination^FA显示文摘Seed dormancy is an adaptive trait in plants. Breaking seed dormancy determines the timing of ger-mination and is, thereby essential for ensuring plant sur-vival and agricultural production. Seed dormancy and the subsequent germination are controlled by both internal cues (mainly hormones) and environmental signals. In the past few years, the roles of plant hormones in regulating seed dormancy and germination have been uncovered. However, we are only beginning to understand how light signaling pathways modulate seed dormancy and inter-action with endogenous hormones. In this review, we summarize current views of the molecular mechanisms by which light controls the induction, maintenance and re-lease of seed dormancy, as well as seed germination, by regulating hormone metabolism and signaling pathways. | Liwen Yang Shuangrong Liu Rongcheng Lin | 2020 | Journal of Integrative Plant Biology2020,62,9: | 11 |
| 4 | GA信号途径及其调控果树生长发育的研究进展显示文摘赤霉素(gibberellins,GAs)是一类重要的植物激素,参与调控植物生长发育的各个阶段,如种子的萌发、幼苗的生长、茎和根的生长及开花等过程。随着分子生物学及遗传学的发展,科学家已经逐步解析了GA在植物体内的信号转导过程及调控植物生长发育的机制。2017年以来,科学家在GA受体GID1的降解机制、DELLA新互作蛋白的鉴定以及O-fucosyltransferase修饰对GA信号的影响等多个方面均有重要发现,这些新成果大大扩展了人们对GA信号调控机制的认知。GA在果树育种中也发挥着重要作用,早在16世纪育种家就在葡萄中成功利用GA信号途径阻遏蛋白DELLA的功能获得型突变实现矮化育种,提高产量。近年来,又在多种果树中发现了GA途径基因突变造成的矮化材料。GA突变体在果树育种中的利用已证明,人为调控GA信号是实现果树高产稳产的有效方法。鉴于GA在植物生长发育及果树生产中的重要作用,笔者将对GA信号途径的最新研究进展及其在果树生产中的应用进行系统介绍,为将来在果树中更高效、更广泛地利用GA途径基因、提高果树产量及品质提供参考。 | 王弋 董晨 魏永赞 郑雪文 李伟才 | 2018 | 果树学报2018,35,4: | 8 |
| 5 | Dof基因家族调节植物生长发育功能的研究进展显示文摘Dof(DNA binding with one finger)蛋白是一类植物特异性转录因子,通常含有200~400个氨基酸和2个主要结构域。该家族成员的N-末端为高度保守的单锌指Dof结构域,具有与DNA和蛋白质相互作用的双重功能,其C末端的氨基酸序列则较为多变,是Dof蛋白重要的特异转录调控结构域。研究表明,Dof蛋白作为转录激活物或阻遏物参与了多方面的植物生长发育过程。随着基因组测序技术的发展,已有大量的Dof基因从植物基因组数据库中鉴定出来。该文对近年来国内外有关Dof基因家族的结构特点、全基因组鉴定、蛋白互作以及生物学功能等方面的研究进展进行综述,以期为Dof转录因子的深入研究提供参考。 | 李娅 丁文杰 江海燕 李玉立 王良桂 岳远征 | 2018 | 西北植物学报2018,38,9: | 7 |
| 6 | The apple DNA-binding one zinc-finger protein MdDof54 promotes drought resistance显示文摘DNA-binding one zinc-finger(Dof)proteins constitute a family of transcription factors with a highly conserved Dof domain that contains a C2C2 zinc-finger motif.Although several studies have demonstrated that Dof proteins are involved in multiple plant processes,including development and stress resistance,the functions of these proteins in drought stress resistance are largely unknown.Here,we report the identification of the MdDof54 gene from apple and document its positive roles in apple drought resistance.After long-term drought stress,compared with nontransgenic plants,MdDof54 RNAi plants had significantly shorter heights and weaker root systems;the transgenic plants also had lower shoot and root hydraulic conductivity,as well as lower photosynthesis rates.By contrast,compared with nontransgenic plants,MdDof54-overexpressing plants had higher photosynthesis rates and shoot hydraulic conductivity under long-term drought stress.Moreover,compared with nontransgenic plants,MdDof54-overexpressing plants had higher survival percentages under short-term drought stress,whereas MdDof54 RNAi plants had lower survival percentages.MdDof54 RNAi plants showed significant downregulation of 99 genes and significant upregulation of 992 genes in response to drought,and 366 of these genes were responsive to drought.We used DAPseq and ChIP-seq analyses to demonstrate that MdDof54 recognizes cis-elements that contain an AAAG motif.Taken together,our results provide new information on the functions of MdDof54 in plant drought stress resistance as well as resources for apple breeding aimed at the improvement of drought resistance. | Pengxiang Chen Mingjia Yan Lei Li Jieqiang He Shuangxi Zhou Zhongxing Li Chundong Niu Chana Bao Fang Zhi Fengwang Ma Qingmei Guan | 2020 | Horticulture Research2020,7,1: | 7 |
| 7 | Regulation of Seed Germination:The Involvement of Multiple Forces Exerted via Gibberellic Acid Signaling显示文摘Seed germination is a key step in the life cycle of all seed plants, and it is of immense significance in agriculture.Germination is controlled by multiple environmental cues such as light and moisture,and endogenous factors,especially phytohormones.Gibberellic acid (GA)has long been identified as a critical hormone in the regulation of seed germination by countering the inhibition imposed by abscisic acid (ABA)(Holdsworth et al.,2008;Lee et al.,2010). | Pratibha Ravindran Prakash P.Kumar | 2019 | Molecular Plant2019,12,1: | 4 |
| 8 | 高等植物中DELLA蛋白的研究进展显示文摘在赤霉素(GA)信号传导过程中,有一类起着负调控作用的蛋白——DELLA蛋白,是GRAS家族的亚家族,因其N端含有DELLA结构域而命名。该蛋白对种子萌发、茎的伸长、下胚轴伸长以及花发育等起着重要的调控作用。本研究主要讲述了DELLA蛋白如何参与多种信号传导,分析了模式植物拟南芥中5种DELLA蛋白对植物生长发育的作用,分别介绍了DELLA蛋白的保守结构域及其作用,总结了目前已知DELLA蛋白的克隆及表达情况,最后对今后DELLA蛋白的研究进行展望,以期更好地揭示DELLA蛋白的调控机制。 | 王倩 杨凤萍 张秀海 肖伟 董然 | 2019 | 分子植物育种2019,17,10: | 3 |
| 9 | 红花CtGATA21基因的克隆及原核表达分析显示文摘植物在整个生命周期中会遇到各种各样的非生物胁迫,而转录因子在植物响应非生物胁迫过程中发挥重要功能。GATA是一类能够参与细胞分化、生长发育和响应胁迫的转录因子,目前还没有广泛的研究。本试验从菊科植物红花中克隆并获得了一个B类GATA基因(命名为Ct GATA21, NCBI登录号:MN399373)。实验结果显示CtGATA21基因全长966 bp,编码321个氨基酸,理论分子量为36.02 kDa,且定位于细胞核中。进一步分析发现,红花CtGATA21的表达具有组织特异性,在叶片中表达量最高;而在盐、干旱胁迫下其表达量迅速积累,显著上调,表明红花CtGATA21能够正响应盐和干旱胁迫,这可能为进一步分析植物GATA转录因子的抗逆功能提供了新的数据。 | 王娟 石必显 胡佳蕙 侯献飞 顾元国 李强 贾东海 | 2020 | 植物生理学报2020,56,3: | 2 |
| 10 | Post-transcriptional regulation of seed dormancy and germination: Current understanding and future directions显示文摘Seed dormancy is a developmental checkpoint that prevents mature seeds from germinating under conditions that are otherwise favorable for germination.Temperature and light are themost relevant environmental factors that regulate seed dormancy and germination.These environmental cues can trigger molecular and physiological responses including hormone signaling,particularly that of abscisic acid and gibberellin.The balance between the content and sensitivity of these hormones is the key to the regulation of seed dormancy.Temperature and light tightly regulate the transcription of thousands of genes,as well as other aspects of gene expression such asmRNAsplicing,translation,and stability.Chromatin remodeling determines specific transcriptional outputs,and alternative splicing leads to different outcomes and produces transcripts that encode proteins with altered or lost functions.Proper regulation of chromatin remodeling and alternative splicing may be highly relevant to seed germination.Moreover,microRNAs are also critical for the control of gene expression in seeds.This review aims to discuss recent updates on post-transcriptional regulation during seed maturation,dormancy,germination,and post-germination events.We propose future prospects for understanding howdifferent post-transcriptional processes in crop seeds can contribute to the design of genotypes with better performance and higher productivity. | Rocío Soledad Tognacca Javier Francisco Botto | 2021 | Plant Communications2021,2,4: | 1 |
| 11 | 大豆DELLA基因家族鉴定及分析显示文摘DELLA基因家族是调控植物与环境互作和植物发育的重要调控因子,可以促进微生物与植物共生关系建立过程中侵染线的形成,是参与赤霉素信号通路的负调控蛋白,在影响植物激素相关基因表达,调节植物与微生物共生固氮和生长发育方面具有重要的作用,但是在大豆中DELLA基因家族的结构特征还没有详细分析。本研究对大豆中DELLA基因家族的基因结构、定位信息、蛋白结构、保守基序分析、系统进化树、顺势作用元件、与拟南芥同源基因的共线性关系和基因表达模式进行了研究。结果发现,大豆基因组中共有7个DELLA基因家族成员,分布在7条不同的染色体上,这些基因都只有一个外显子,一个N端DELLA结构域,并且基序分布相似,证明其基因编码序列结构域高度保守。系统进化树分析表明DELLA家族有三个亚族,其启动子区域含有大量的顺式作用元件,这些元件参与植物激素反应、干旱诱导和光反应。大豆DELLA基因Glyma.11G216500、Glyma.18G040000、Glyma.08G095800和Glyma.05G140400与拟南芥中的AT1G14920、AT1G66350和AT2G01570呈共线性关系;其中Glyma.18G040000和Glyma.11G216500在大豆各个组织中都有表达,而且表达量相对较高。以上研究丰富了我们对大豆DELLA基因家族的理解,为后续大豆DELLA基因的功能研究奠定了基础。 | 梁帅 陈庆山 朱子坤 李冬冬 齐照明 辛大伟 | 2022 | 中国油料作物学报2022,44,5: | 0 |
| 12 | To Bring Flowers or Do a Runner:, Gibberellins Make the Decision显示文摘 | Auxiliadora O. Martins Adriano Nunes-Nesi Wagner L. Aradjo Alisdair R. Femie | 2018 | Molecular Plant2018,11,1: | 0 |