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| 1 | Dof转录因子在作物逆境胁迫响应及农艺性状改良中的作用显示文摘阐述DNA-binding with one finger(Dof)转录因子在作物中应答极端温度、干旱和盐胁迫等逆境的分子机制及其改良农艺性状的作用;介绍Dof转录因子在作物中响应逆境的信号感知方式和信号转导途径,分析其基因表达调控过程的调控机理以及对胁迫耐受力与重要次生代谢产物(RFOs等)等积累的影响。同时从多信号网络调控机制以及全球气候变化对作物可持续和高质量生产的需求角度,对进一步研究和利用Dof转录因子进行作物生物育种的前景和方向作展望。 | 王泽民 晋昕 张飞燕 司怀军 | 2023 | 生物学杂志2023，40，4： | 1 |
| 2 | 农业抗旱策略及苎麻相关研究建议显示文摘缺水是限制作物生产能力、困扰农业发展的全球性问题。苎麻是我国特色经济作物,抗旱和水土保持能力强,是有效利用南方干旱坡地的先锋作物,但苎麻产量与品质受干旱影响严重。研究苎麻应答干旱胁迫的机制及其农业应对策略,将为苎麻稳产高产提供理论与技术支撑。文章在探讨干旱胁迫影响植物生产的一般性规律基础上,总结了苎麻抗旱研究的主要进展与问题,以期为苎麻抗旱育种与生产提供依据。 | 赵浩含 朱爱国 陈继康 | 2022 | 中国麻业科学2022，44，5： | 0 |
| 3 | Insights into the molecular mechanisms underlying responses of apple trees to abiotic stresses显示文摘Apple(Malus×domestica)is a popular temperate fruit crop worldwide.However,its growth,productivity,and quality are often adversely affected by abiotic stresses such as drought,extreme temperature,and high salinity.Due to the long juvenile phase and highly heterozygous genome,the conventional breeding approaches for stress-tolerant cultivars are time-consuming and resource-intensive.These issues may be resolved by feasible molecular breeding techniques for apples,such as gene editing and marker-assisted selection.Therefore,it is necessary to acquire a more comprehensive comprehension of the molecular mechanisms underpinning apples’response to abiotic stress.In this review,we summarize the latest research progress in the molecular response of apples to abiotic stressors,including the gene expression regulation,protein modifications,and epigenetic modifications.We also provide updates on new approaches for improving apple abiotic stress tolerance,while discussing current challenges and future perspectives for apple molecular breeding. | Xuewei Li Ziqing Ma Yi Song Wenyun Shen Qianyu Yue Abid Khan Muhammad Mobeen Tahir Xiaofei Wang Mickael Malnoy Fengwang Ma Vincent Bus Shuangxi Zhou Qingmei Guan | 2023 | Horticulture Research2023，10，8： | 0 |
| 4 | Control of ovule development in Vitis vinifera by VvMADS28 and interacting genes显示文摘Seedless grapes are increasingly popular throughout the world,and the development of seedless varieties is a major breeding goal.In this study,we demonstrate an essential role for the grapevine MADS-box gene VvMADS28 in morphogenesis of the ovule.We found that VvMADS28 mRNA accumulated in the ovules of a seeded cultivar,‘Red Globe’,throughout the course of ovule and seed development,especially within the integument/seed coat.In contrast,in the seedless cultivar‘Thompson Seedless’,VvMADS28 was expressed only weakly in ovules,and this was associated with increased levels of histone H3 lysine 27 trimethylation(H3K27me3)within the VvMADS28 promoter region.RNAi-mediated transient suppression of VvMADS28 expression in‘Red Globe’led to reduced seed size associated with inhibition of episperm and endosperm cell development.Heterologous overexpression of VvMADS28 in transgenic tomatoes interfered with sepal development and resulted in smaller fruit but did not obviously affect seed size.Assays in yeast cells showed that VvMADS28 is subject to regulation by the transcription factor VvERF98,and that VvMADS28 could interact with the Type I/MβMADS-domain protein VvMADS5.Moreover,through DNA-affinity purification-sequencing(DAP-seq),we found that VvMADS28 protein specifically binds to the promoter of the grapevine WUSCHEL(VvWUS)gene,suggesting that maintenance of the VvMADS28–VvMADS5 dimer and VvWUS expression homeostasis influences seed development.Taken together,our results provide insight into regulatory mechanisms of ovule and seed development associated with VvMADS28. | Songlin Zhang Li Wang Jin Yao Na Wu Bilal Ahmad Steve van Nocker Jiuyun Wu Riziwangguli Abudureheman Zhi Li Xiping Wang | 2023 | Horticulture Research2023，10，6： | 0 |
| 5 | 染色质免疫共沉淀测序技术及其在园艺植物中的应用研究进展显示文摘近年来,基于染色质免疫共沉淀技术(ChIP)与第二代测序技术结合的染色质免疫共沉淀测序技术(ChIP-seq)已被广泛用于研究DNA与蛋白质的相互作用,对解析园艺植物基因的表达与调控、靶基因筛选与定位、调控网络构建、染色质开放程度确认等方面具有较大的优势。本文综述了ChIP-seq技术的发展简史及其在园艺植物转录因子和组蛋白修饰中所取得的进展,以期为园艺植物DNA—蛋白互作研究提供参考。 | 翟挺楷 马湘玮 陈燕 张雪莹 李卓蕴 徐卢振 傅卓然 赖钟雄 林玉玲 | 2024 | 园艺学报2024，51，1： | 0 |
| 6 | 蓖麻矮化相关RcDof蛋白的原核表达及纯化显示文摘目的原核表达并纯化蓖麻矮化相关RcDof蛋白。方法利用SignalP 4.0 server和Iprscan软件分别预测RcDof基因的信号肽和结构域。从pMD-18T-RcDof质粒中PCR扩增RcDof(44-101)基因,克隆入原核表达载体pETMBP-XE,构建原核表达质粒pETMBP-XE-RcDof(44-101),IPTG诱导表达重组蛋白,利用亲和层析、阳离子交换层析以及分子筛层析对目的蛋白进行纯化。结果RcDof基因无信号肽剪切序列,在44~101个碱基之间存在1个锌指保守结构域。重组表达质粒经双酶切及测序鉴定证明构建正确。表达的重组蛋白相对分子质量约43000,以可溶形式存在于上清中。纯化的重组蛋白纯度较高,浓度为10 mg/mL。结论获得了高纯度可溶RcDof蛋白,为解析其晶体结构及探索其在蓖麻中作用的分子机制提供了实验依据。 | 张帅 王双 李跃 李国瑞 黄凤兰 陈永胜 | 2022 | 中国生物制品学杂志2022，35，4： | 0 |
| 7 | 翠冠梨大果芽变果实组织切片、激素变化及转录组分析显示文摘【目的】芽变材料是果树育种中的重要资源。前期发现1个翠冠梨的大果芽突变体,果实明显大于普通翠冠,对其变异来源和分子机制进行探究。【方法】通过石蜡切片、内源激素测定以及转录组测序解析大果芽变机制。【结果】石蜡切片结果显示,大翠冠果肉细胞横切面积大于翠冠,说明大翠冠的细胞体积增大。大翠冠中反式玉米素和赤霉素A3的含量分别在开花后10和20 d高于翠冠。花后40 d内分5个时间点取样,利用RNA-seq高通量测序评估幼果发育期间的基因表达水平,共鉴定出2015个差异表达基因,其中302个基因在2个以上时间点发生差异表达。在花后0和10 d时,一些植物激素信号转导相关基因发生差异表达。在大翠冠中3个细胞分裂素相关基因(AHP1、ORR10和ARR17)花后10 d上调表达,在大翠冠中赤霉素负调控相关基因GA3ox1和GA2ox1分别在花后0和10 d下调表达。在大翠冠中发现1个长链非编码lncRNA在所有时间点均下调表达。在大翠冠中晚期胚胎富集蛋白基因D29、锌指蛋白基因dof2.1和伸展蛋白基因extensin等在一些时间点发生表达上调,表明这些基因可能在控制果实细胞大小中起重要作用。【结论】该研究结果从生理生化和分子水平揭示了翠冠梨大果芽变可能的变异原因,细胞分裂素和赤霉素可能参与调控果实的大小,转录组分析为解析翠冠梨大果芽变的分子机制奠定了基础。 | 蒋爽 骆军 王晓庆 李水根 周博强 | 2022 | 果树学报2022，39，10： | 0 |
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