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| 1 | 运用“三因制宜”理论指导慢性胃炎防治饮食干预探微显示文摘'三因制宜'学说是中医的基本治则之一,是中医整体观念和辨证论治的具体体现。运用三因制宜学说指导慢性胃炎的防治,与现代医学中的个体化营养殊途同归。在前期饮食情况调查问卷分析的基础上,系统梳理了古代及近、现代文献中有关慢性胃炎常见主症('胃痛''痞满''吐酸')的常用方剂、药物以及食疗的方法,总结慢性胃炎饮食致病理论及饮食调治方案,在已经建立的慢性胃炎临床症状观察表和疗效评价体系的基础上,制定了中医三因制宜理论指导下与慢性胃炎疾病特征相契合的基于临床的慢性胃炎防治食疗干预方案,同时结合中医药三因制宜的理论,对慢性胃炎不同证候的临床患者,给予了综合三因制宜和中医辨证的饮食方。通过对患者定期随访,在临床上也初步看到了饮食干预方案在缓解患者临床症状、延缓疾病进展等方面的良好疗效。 | 王万卷 李园 杜世豪 李依聪 苏泽琦 曹雅蕾 李萍 丁霞 | 2019 | 世界中西医结合杂志2019，14，2： | 12 |
| 2 | 胃癌干细胞在胃癌侵袭、转移中及血管形成中的作用和机制显示文摘背景:肿瘤干细胞及其分化的血管细胞对于化疗、分子靶向治疗并不敏感,虽然能够减少肿瘤总体的血供,却助长了肿瘤的浸入及转移,成为治疗失败的主要机制。目的:分析胃癌干细胞在胃癌侵袭、转移中的机制及其在血管形成中的作用。方法:取20株胃癌肿瘤干细胞CSC-G和普通胃癌细胞SGC7901细胞,普通癌细胞培养条件为10%的PBS;肿瘤干细胞CSC-G培养条件为:DMEM/F12(1∶1)、B27(2%)、表皮生长因子(20μg/L)。对2种细胞进行球形克隆形成试验、平板克隆试验以及Transwell侵袭性实验,观察胃癌肿瘤干细胞的侵袭性;测定CD44、E-cadherin中mR NA水平及蛋白表达水平,观察细胞的转移能力;利用细胞划痕试验、Transwell迁移实验和成环实验观察细胞的血管形成能力。结果与结论:(1)普通癌细胞均贴壁生长,肿瘤干细胞CSC-G为悬浮生长,生长后显微镜下显示为梭形间质形态;(2)球形克隆形成试验结果:普通癌细胞瘤球数量显著少于肿瘤干细胞CSC-G(P<0.05);(3)平克隆试验结果:胃癌肿瘤干细胞瘤球数量显著多余普通胃癌细胞(P<0.05);(4)转移性试验结果:胃癌肿瘤干细胞穿过基底膜细胞数显著多于普通胃癌(P<0.05);(5)划痕试验结果:肿瘤干细胞CSC-G迁移距离、成环试验中成环数量显著多于普通胃癌细胞(P<0.05)。(6)结果提示:胃癌肿瘤干细胞侵袭能力高于普通胃癌细胞,且胃癌干细胞迁移能力以及血管成形能力较强,它可以通过形成血管等途径来进一步促进胃癌的发生与发展。 | 黄辉 潘志坚 | 2016 | 中国组织工程研究2016，20，14： | 10 |
| 3 | 胃癌转移分子机制研究进展显示文摘胃癌是消化道最常见的恶性肿瘤之一,当前在我国的发病率有明显增长的趋向。除了显著的地域差异性之外,胃癌存在明显的个体差异,不同患者对治疗的反应不同、疾病进展速度不同。同时胃癌具有死亡率高、手术治疗率低、早期诊断率低、复发率高等特点,因此要在术前加强对胃癌病情的分析,以便更准确地评价胃癌的防治效果,并为改善预后提供可靠的依据。近年来胃癌的手术方式层出不穷,但预后效果仍有待提高,尽管胃癌术后1年生存率为80%,5年生存率为50%,但是复发率超过60%。 | 单磊 汪子书 | 2017 | 包头医学院学报2017，33，10： | 7 |
| 4 | 肠型胃癌的研究进展显示文摘Lauren分型是最常用的胃癌组织病理学分型之一,它将胃癌分为肠型、弥漫型和混合型。我国是胃癌高发区,其中肠型胃癌在我国的发病率明显高于弥漫型胃癌和混合型胃癌。全文分析了近十几年与Lauren分型相关的文献,从流行病学及病因、发病机制、生物学特征等三个方面对肠型胃癌展开讨论,对肠型胃癌的研究进展作一综述。 | 刘霞 杨明 张艳桥 | 2015 | 中国肿瘤2015，24，10： | 7 |
| 5 | microRNA-219-2-3p在胃癌中的表达及其作用机制初探显示文摘目的探讨microRNA-219-2-3p(miR-219-2-3p)与胃癌的相关性及机制。方法运用实时聚合酶链反应(real timeRT PCR)检测82份配对的胃癌组织和周围正常组织样本中的miR-219-2-3p的表达水平。在胃癌细胞株MGC803中过表达miR-219-2-3p后,测定细胞增殖活性,并在细胞株和胃癌组织标本中采用蛋白免疫印迹法(Western blot)测定与肿瘤增殖相关的蛋白ERK1/2表达水平。结果 miR-219-2-3p在晚期胃癌组织中的表达量下降,差异有统计学意义(P<0.05)。在胃癌细胞株MGC803中过表达miR-219-2-3p后细胞增殖受显著抑制(P<0.05)。过表达miR-219-2-3p后,MGC803细胞中的ERK磷酸化水平显著下降,总ERK表达量不变。在组织标本中,胃癌组织的ERK磷酸化(p-ERK)水平明显高于周围癌旁组织。结论 miR-219-2-3p可能通过参与调控ERK1/2信号通路而在胃癌发生和发展中起抑癌基因的作用。 | 金锦莲 吴发明 周海燕 谢晓晶 王新宇 | 2014 | 重庆医学2014，43，14： | 5 |
| 6 | 基质金属蛋白酶9及热休克蛋白60检测对早期胃癌的诊断价值显示文摘目的探讨血浆基质金属蛋白酶9(MMP-9)与热休克蛋白60(HSP-60)联合检测对早期胃癌患者的诊断价值。方法选取我院收治的临床疑诊早期胃癌136例患者,根据患者确诊诊断分为:对照组(70例,排除胃癌诊断)和研究组(66例,确诊胃癌诊断)。检测并比较两组患者MMP-9、HSP-60、CA19-9、CA72-4以及CEA水平差异。采用Logistic回归分析影响早期胃癌发生的临床因素。运用ROC曲线评估MMP-9与HSP-60对诊断早期胃癌患者的最佳值。结果研究组血浆MMP-9、HSP-60、CA19-9、CA72-4与CEA水平均高于对照组(P均<0.05)。多因素Logistic回归分析结果显示,血浆MMP-9与HSP-60是早期胃癌发生的危险因素(P<0.05)。血浆MMP-9与HSP-60诊断早期胃癌的ROC曲线下面积分别为0.781和0.667(P=0.024,0.011)。当MMP-9≥364.5μg/L和HSP60≥2.84μg/L为cut-off值,对判断早期胃癌发生的灵敏度为53.0%,特异性为81.4%,准确度为67.6%,ROC曲线下面积为0.862。结论血浆MMP-9与HSP-60联合检测对早期胃癌发生具有较好的诊断价值。 | 白洁 惠明 梁灿灿 | 2017 | 标记免疫分析与临床2017，24，2： | 5 |
| 7 | Reduced Popdc3 expression correlates with high risk and poor survival in patients with gastric cancer显示文摘AIM:To investigate the expression of Popeye domain containing 3(Popdc3) and its correlation with clinicopathological features and prognosis of gastric cancer.METHODS:The method of immunohistochemistry was used to investigate the expression of Popdc3 in 306 cases of human gastric cancer and 84 noncancerous gastric tissues.Simultaneously,the relationship between Popdc3 expression and the survival of the patients was retrospectively analyzed.RESULTS:Popdc3 was detected in 72(85.71%) of 84 human nontumor mucosa.High expression of Popdc3 protein was detected in 78(25.49%) of 306 human gastric cancer cases,and low expression was detected in 228(74.51%).Low expression of Popdc3 correlated with depth of invasion(P < 0.0001),regional lymph nodes(P < 0.0001) and distant metastasis(P = 0.02),and tumor,nodes,metastasis(TNM) stages(P < 0.0001).On multivariate analysis,only the patient's gender,regional lymph node metastasis,distant metastasis,TNM stages,and the expression of Popdc3 were independent prognostic factors in patients with gastric cancer.The Kaplan-Meier plot showed that low Popdc3 expression had a much more significant effect on the survival of those patients with early-stage tumors(χ 2 = 104.741,P < 0.0001),with a > 51.9% reduction in the three-year survival compared with high Popdc3 expression.In late stages,the difference was also significant(χ 2 = 5.930,P = 0.015),with a 32.6% reduction in the three-year survival.CONCLUSION:Reduced expression of Popdc3 may play a significant role in the carcinogenesis and progression of gastric cancer.Popdc3 may be an independent prognostic factor. | Deng Luo Ming-Liang Lu Gong-Fang Zhao Hua Huang Meng-Yao Zheng Jiang Chang Lin Lv Jin-Bo Luo | 2012 | World Journal of Gastroenterology2012，18，19： | 4 |
| 8 | Perspectives on new biomarkers in gastric cancer: Diagnostic and prognostic applications显示文摘Gastric cancer is considered one of the most deadly tumors worldwide. Even with the decline in its incidence, the mortality rate of this disease has remained high, mainly due to its late diagnosis and to the lack of precise prognostic markers. The main purpose of this review is to present genetic, epigenetic and proteomic molecular markers that may be used in a diagnostic and prognostic manner and to discuss the pros and cons of each type of marker for improving clinical practice. In this sense, we observed that the use of genetic markers, especially mutations and polymorphisms, should be carefully considered, as they are strongly affected by ethnicity. Proteomic-based markers show promise, but the higher costs of the associated techniques con-tinue to make this approach expensive for routine use. Alternatively, epigenetic markers appear to be very promising, as they can be detected in bodily fluids as well as tissues. However, such markers must be used carefully because epigenetic changes may occur due to environmental factors and aging. Despite the advances in technology and its access, to date, there are few defined biomarkers of prognostic and diagnostic use for gastric tumors. Therefore, the use of a panel of several approaches(genetic, epigenetic and proteomic) should be considered the best alternative for clinical practice. | Danilo do Rosário Pinheiro Wallax Augusto Silva Ferreira Mariceli Baia Leo Barros Mariana Diniz Araújo Symara Rodrigues-Antunes Bárbara do Nascimento Borges | 2014 | World Journal of Gastroenterology2014，20，33： | 4 |
| 9 | 基于医院信息系统电子医疗数据的复方苦参注射液治疗胃恶性肿瘤真实世界临床应用特征研究显示文摘目的:基于医院信息系统(HIS)电子医疗数据,解析真实世界复方苦参注射液治疗胃恶性肿瘤临床应用特征,为复方苦参注射液的临床合理应用提供参考借鉴。方法:基于中国中医科学院中医临床基础医学研究所建立的HIS电子医疗数据大型集成数据仓库,对来自全国范围22家大型三甲医院使用复方苦参注射液治疗胃恶性肿瘤的3 981例患者电子医疗数据进行提取,对一般特征、诊断特征、品种用药剂量与疗程特征信息、品种联合用药特征、出院转归特征等进行基于频数与率的描述性分析。结果:使用复方苦参注射液治疗胃恶性肿瘤的患者年龄集中在45-65岁;男性多于女性;主要由消化内科、肿瘤科入院;单次用药剂量以10-20m L居多;疗程以4-14d为主;临床常见联用药物包括地塞米松、托烷司琼注射液、胸腺肽注射液、复方氨基酸注射液、泮托拉唑钠注射液、氟尿嘧啶等;基于出院转归判定的治疗总有效率42.87%。结论:复方苦参注射液治疗胃恶性肿瘤的人群特征明确,符合胃恶性肿瘤疾病的一般规律;其真实世界治疗胃恶性肿瘤临床用药剂量、疗程范围基本符合品种说明书相关界定;临床联合用药类型较为广泛。 | 刘亚欣 谢雁鸣 张寅 陈岑 庄严 | 2018 | 中华中医药杂志2018，33，4： | 4 |
| 10 | Risk assessment models of integrative medicine indicators for malignant transformation of chronic atrophic gastritis:A registry study protocol显示文摘Objective:Traditional Chinese medicine(TCM)and modern medicine have both been used in arresting malignant transformation of chronic atrophic gastritis(CAG)in China with good therapeutic effect.However,no studies have been undertaken to assess the risk of malignant transformation in CAG patients using both modern medicine and TCM features.Our study aimed to develop risk assessment models for malignant transformation of CAG combining indicators of both TCM and modern medicine.These models will facilitate early warning and control of malignant transformation of CAG from the perspective of evidence-based integrative medicine.Methods:In the proposed registry study,a total of 1000 eligible CAG patients will be recruited from four hospitals in China.A 10-year follow-up study will be conducted both on-site and off-site to track the events of malignant transformation.Frequency analysis and chi-squared tests will be used to perform the comparative analysis on the prevalence of malignant transformation events and indicators in TCM or modern medicine in different groups.A multivariate Cox proportional hazard model will be used to perform correlation analyses of malignant transformation events and factors of TCM or modern medicine.Conclusion:The proposed study has been designed with a large sample size and long follow-up period,in which wide-ranging modern medicine and TCM indicators can be gathered over the whole process of malignant transformation of CAG.Based on this study,risk assessment models for malignant transformation ofCAGmaybe constructed fromthe perspective of integrative medicine.This may provide clinicians and patients with an optimized early warning system as well as prevention strategies for malignant transformation of CAG. | Yue Liu Wanjuan Wang Yuan Li Fusheng Liu Li Zhang Tao Tang Mingxuan Zang Yin Zhang Xia Ding | 2019 | Journal of Traditional Chinese Medical Sciences2019，6，3： | 3 |
| 11 | Increased expression of DLX2 correlates with advanced stage of gastric adenocarcinoma显示文摘AIM:To investigate the expression of distal-less homeobox 2 (DLX2) in gastric adenocarcinoma and its clinicopathological significance. METHODS:Gastric adenocarcinoma tissues were obtained from gastrectomy specimens of 129 patients from the Department of Surgery and Pathology, the Second Affiliated Hospital of Kunming Medical University. Sixty cases of normal gastric tissues were collected from gastrectomy specimens of adjacent gastric cancer margins greater than 5 cm. Patient diagnosis was established pathologically, and no patient had received chemotherapy or radiotherapy before surgery. All tissue specimens were formalin-fixed and paraffin-embedded. Immunohistochemistry was carried out to investigate the expression of DLX2 in 129 gastric adenocarcinoma tissues and 60 adjacent normal tissues. The immunos-taining reaction was semiquantitatively evaluated based on the proportion of positive cells and the median staining intensity in normal gastric epithelial cells or tumor cells. All patients had follow-up records for more than 5 years. Correlations of DLX2 expression with clinicopathological features and prognosis of patients with gastric adenocarcinoma were analyzed. All statistical analyses were performed using the SPSS 17.0 software. RESULTS:The positive expression of DLX2 was detected in 68 (52.7%) cases of 129 gastric adenocarcinoma tissues and 14 (23.3%) cases of 60 adjacent normal tissues. The difference in DLX2 expression between gastric adenocarcinoma tissues and adjacent normal tissues was statistically significant (Ⅹ2 = 14.391, P < 0.001). Moreover, high expression of DLX2 was detected in 48 (37.2%) cases of 129 human gastric cancer tissues, but not in adjacent normal tissues. The expression of DLX2 correlated with the size of tumor (P = 0.001), depth of invasion (P = 0.008), lymph node metastasis (P = 0.023) and tumor-node-metastasis stages (P = 0.020), but was not correlated with age, gender, histological differentiation and distant metastasis. The Kaplan-Meier survival analysis revealed that survival time of patients with high DLX2 expression was significantly shorter than that with low DLX2 expression. However, the multivariate analysis showed that invasion depth (P < 0.001), lymph nodes metastasis (P = 0.001) and distant metastasis (P < 0.001) were independent prognostic factors for patients with gastric adenocarcinoma, but DLX2 expression, tumor location and tumor size were not. CONCLUSION:These results suggest that increased expression of DLX2 may correlate with the advanced stage of gastric adenocarcinoma, and it may contribute to tumor development. | Peng Tang Hua Huang Jiang Chang Gong-Fang Zhao Ming-Liang Lu Yan Wang | 2013 | World Journal of Gastroenterology2013，19，17： | 3 |
| 12 | miRNA调控胃癌细胞侵袭和转移的研究进展显示文摘胃癌是我国常见的消化系统恶性肿瘤之一,肿瘤发生的早期就容易发生淋巴转移和血液转移,肿瘤远处转移是胃癌患者主要死亡原因,但目前胃癌侵袭转移的分子机制尚未完全阐明。MicroRNA(miRNA)是一类内源性非编码小RNA,在转录后水平调控基因的表达。许多研究已经证实,miRNA在多种类型肿瘤中存在异常表达,miRNA通过与胃癌相关的调控基因或mRNA结合,使目的mRNA表达沉默或增强,导致胃癌细胞的侵袭和转移。本文就miRNA在胃癌细胞侵袭和转移中的作用机制的相关研究作一综述。 | 李厚雷 吴宏 | 2017 | 世界最新医学信息文摘2017，0，60： | 3 |
| 13 | 肠型胃癌组织异常表达miRNAs的鉴定显示文摘目的筛选肠型胃癌组织异常表达的miRNAs。方法采用miRCURYTM基因芯片(v.14.0)分析6例肠型胃癌组织和邻近非肿瘤组织之间差异表达的miRNA,设定平均上升或下降倍数大于2倍和P值小于0.01为显著性差异标准;选取部分基因芯片分析中呈异常表达的miRNAs,采用实时荧光定量PCR(RT-qPCR)方法在29例肠型胃癌组织和邻近非肿瘤组织中进一步检测,并对两者结果进行相关性分析。结果基因芯片结果显示,40个miRNAs呈显著表达上调,其中24个基因已证实在胃癌癌组织中表达升高;36个miRNAs呈显著表达下调,其中19个已报道在胃癌组织中异常表达降低。用RT-qPCR检测6个在基因芯片分析中表达上调的miRNAs和5个在基因芯片分析中表达下调的miRNAs,结果与基因芯片分析结果相一致,两种方法分析的结果呈高度正相关(P<0.01)。结论该研究鉴定了肠型胃癌组织中一系列新的异常表达的miRNAs,为进一步研究提供了基础。 | 李新华 张桂英 李乾 徐美华 冯德云 吴畏 | 2011 | 中国普通外科杂志2011，20，9： | 3 |
| 14 | Mitochondrial DNA alterations in the progression of gastric carcinomas:Unexplored issues and future research needs显示文摘Gastric cancer is the second most frequent cause of cancer death worldwide.Patients infected with Helicobacter pylori(H.pylori)are at increased risk of gastric cancer.H.pylori induces genomic instability in both nuclear and mitochondrial(mt)DNA of gastric epithelial cells.Changes in mt DNA represent an early event during gastric tumorigenesis,and thus may serve as potential biomarkers for early detection and prognosis in gastric carcinoma.This review article summarizes the mt DNA mutations that have been reported in gastric carcinomas and their precancerous conditions.Unexplored research topics,such as the role of mt DNA alterations in an alternative pathway of gastric carcinogenesis,are identified and directions for future research are suggested. | Luciana Rigoli Rosario Alberto Caruso | 2014 | World Journal of Gastroenterology2014，20，43： | 2 |
| 15 | 胃癌组织中HSP60和MMP9的表达及临床意义显示文摘[目的]探讨HSP60和MMP9在胃癌中的表达及与临床病理参数的关系,评价HSP60和MMP9在胃癌发生、发展中的作用及其意义。[方法]采用免疫组化方法检测HSP60和MMP9在65例胃癌患者手术标本中及在20例相应癌旁正常组织中的表达情况。[结果]HSP60和MMP9在胃癌组织中的阳性率分别为63.1%、67.8%,明显高于在正常组织中的表达,差异有统计学意义(P<0.01)。HSP60与胃癌的浸润深度、淋巴结转移和临床分期有关,MMP9与胃癌的浸润深度、分化程度、临床分期、淋巴结转移有显著的相关性,HSP60与MMP9的表达呈正相关(r=0.289 P=0.019)。[结论]HSP60和MMP9表达与胃癌的临床分期、淋巴结转移有关,二者的共同作用可能是促进胃癌恶性进展的重要因素,同时检测HSP60和MMP9的表达水平将有助于判断胃癌的恶性程度及其预后。 | 李桂青 王兵 | 2016 | 临床消化病杂志2016，28，1： | 2 |
| 16 | 胃癌及癌前病变微卫星不稳定的研究显示文摘目的 探讨胃癌及癌前病变微卫星不稳定(MSI)的存在与否及其作用.方法 利用激光显微切割技术分别收集胃癌、肠化、异型增生及正常胃黏膜,选择5个微卫星位点应用变性高效液相色谱技术检测MSI.结果 5个微卫星位点REF阳性表型中,肠化MSI为10.5%,异型增生MSI为21.12%,胃癌MSI为43.75%,正常胃黏膜无MSI.结论 激光捕获显微切割技术有效地解决了异质性问题.变性高效液相色谱技术检测MSI具有半自动化、快速、检出率高等优点.MSI从癌前到胃癌逐步增加,癌前MSI的检测对胃癌的早期诊断可能是一个潜在的预警指标. | 张伟 | 2013 | 肿瘤研究与临床2013，25，10： | 2 |
| 17 | KAI1/CD82在胃癌进展中的研究显示文摘目的 检测癌前病变和不同分化阶段胃癌标本中KAI1基因杂合性缺失(LOH)、微卫星不稳定(MSI)、mRNA和其蛋白表达情况.方法 分别应用PCR-单链构象多态(PCR-SCCP)、原位杂交和免疫组化等方法检测癌前病变与高、中、低分化胃癌、正常对照组相关指标.结果KAI1 mRNA和其蛋白表达分别与癌前病变和高、中、低分化胃癌有显著性相关(P〈0.05).60例胃癌标本D11S1344、D11S1326、D11S554位点的SSCP分析中,均未出现MSI或LOH.结论 KAI1/CD82 基因缺失或表达降低与胃癌的发生、发展密切相关,但是具体作用机制还需进一步深入研究. | 康丽霞 李闯 王献春 冯艳玲 | 2017 | 临床研究2017，25，5： | 1 |
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