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| 1 | Effect of Boschniakia rossica on expression of GSTP,p53 and p21^(ras)proteins in early stage of chemical hepatocarcinogenesis and its anti-inflammatory activities in rats显示文摘AIM To investigate the effect of Boschniakiarossica(BR)extract on expression of GST-P,p53 and p21rasproteins in early stage of chemicalhepatocarcinogenesis in rats and its anti-inflammatory activities.METHODS The expression of tumor marker-placental form glutathione S-transferase(GST-P),p53 and p21rasproteins were investigated byimmunohistochemical techniques and ABCmethod.Anti-inflammatory activities of BR werestudied by xylene and croton oil-induced mouseear edema,carrageenin,histamine and hotscald-induced rat pow edema,adjuvant-inducedrat arthritis and cotton pellet-induced mousegranuloma formation methods.RESULTS The 500 mg/kg of BR-H2O extractfractionated from BR-Methanol extract hadinhibitory effect on the formation of DEN-inducedGST-P-positive foci in rat liver(GST-P stainingwas 78% positive in DEN+AAF group vs 20%positive in DEN+AAF+BR group,P<0.05)andthe expression of mutant p53 and p21rasproteinwas lower than that of hepatic preneoplasticlesions(33% and 22% positive respectively inDEN+AAF group vs negative in DEN+AAF+BRgroup).Both CH2Cl2 and H2O extracts from BRhad anti-inflamatory effect in xylene and crotonoil-induced mouse ear edema(inhibitory rateswere 26%-29% and 35%-59%,respectively). BR-H2O extract exhibited inhibitory effect incarrageenin,histamine and hot scald-inducedhind paw edema and adjuvant-induced arthritis inrats and cotton pellet-induced granulomaformation in mice.CONCLUSION BR extract exhibited inhibitory effect on formation of preneoplastic hepatic foci in early stage of rat chemical hepato-carcinogenesis. Both CH2CI2 and H2O extracts from BR exerted anti-inflammatory effect in rats and mice. | Zong Zhu Yin Hai Ling Jin Xue Zhe Yin Tian Zhu Li Ji Shu Quan Zeng Nan Jin Institute for Cancer Research,Yanbian University College of Medicine,Yanji 133000,Jilin Province,China | 2000 | World Journal of Gastroenterology2000，6，6： | 33 |
| 2 | Heat and power load dispatching considering energy storage of district heating system and electric boilers显示文摘As one of promising clean and low-emission energy, wind power is being rapidly developed in China.However, it faces serious problem of wind curtailment,particularly in northeast China, where combined heat and power(CHP) units cover a large proportion of the district heat supply. Due to the inherent strong coupling between the power and the heat load, the operational flexibility of CHP units is severely restricted in winter to meet the heat supply demand, which imparts considerable stress on the wind power connection to the grid. To promote the integration of wind power and enhance the flexibility of CHP units, this paper presented a method of heat and power load dispatching by exploring the energy storage ability of electric heating boilers and district heating systems. The optimization results indicate that the proposed method can integrate additional wind power into the grid and reduce the coal consumption of CHP units over the optimized period. Furthermore, the thermal inertia of a district heating system is found to contribute more to the reduction of coal consumption, whereas the electric heating boilers contribute to lower wind curtailment. | Xianzheng HUANG Zhaofeng XU Yong SUN Yali XUE Zhe WANG Zhijun LIU Zhenyuan LI Weidou NI | 2018 | Journal of Modern Power Systems and Clean Energy2018，6，5： | 20 |
| 3 | Oligonucleotide-based targeted gene editing in C. elegans via the CRISPR/Cas9 system显示文摘 | Pei Zhao Zhe Zhang Hongmei Ke Yiren Yue Ding Xue | 2014 | Cell Research2014，24，2： | 17 |
| 4 | Predictive value of N-terminal pro-brain natriuretic peptide in combination with the sequential organ failure assessment score in sepsis显示文摘 | JU Min-jie ZHU Du-ming TU Guo-wei HE Yi-zhou XUE Zhang-gang LUO Zhe WU Zhao-guang | 2012 | Chinese Medical Journal2012，，11： | 11 |
| 5 | Cutting-balloon angioplasty before drug-eluting stent implantation for the treatment of severely calcified coronary lesions显示文摘BackgroundSeverely 钙化的冠的损害对汽球 angioplasty 糟糕作出回应,导致不完全、不均匀的 stent 扩大。因此,在 drug-eluting stent (DES ) 以前的足够的匾修正培植是为钙化的损害治疗的钥匙。这研究是评估为严重地钙化的冠的 lesions.MethodsNinety 切汽球 angioplasty 的安全和功效 -- 有严重地钙化的损害的二个连续病人(定义为钙弧 ≥180 °钙长度比率 ≥0.5 ) 在 DES 培植随机基于汽球类型被划分成二个组以前,与汽球膨胀对待:在常规汽球 angioplasty (BA ) 的 45 个病人组织,在切的汽球 angioplasty (CB ) 的 47 个病人组织。在 BA 组的七个盒子令人满意地没完成膨胀并且被变成 CB 组。 Intravascular 超声( IVUS )在汽球膨胀前并且在 stent 培植以后被执行获得质、量的损害特征并且评估 stent ,包括最小的腔代表性的区域( CSA ),钙化的弧和长度,最小的 stent CSA , stent 并置, stent 对称, stent 扩大,容器解剖,并且分叉容器监狱。在里面医院, 1 月、 6 月的主要不利心脏的事件(向) 是 reported.ResultsThere 不是在在二个组之间的临床的特征的统计差别,包括钙弧(222.2 °±22.2 °对 235.0 °±22.1 °, P = 0.570 ) ,钙长度比率(0.67 ±0.06 对 0.77 ±0.05, P = 0.130 ) ,并且在一种总线标准前的最小的腔 CSA (2.59 ±0.08 公里 2 对 2.52 ±0.08 公里 2, P = 0.550 ) 。在 stent 培植以后,最后的最小的 stent CSA (6.26 ±0.40 公里 2 对 5.03 ±0.33 公里 2 ;P = 0.031 ) 并且尖锐的腔获得(3.74 ±0.38 公里 2 对 2.44 ±0.29 公里 2, P = 0.015 ) 比 BA 组的在 CB 组是显著地更大的。没有统计上在在二个组之间的 stent 扩大, stent 对称,不完全的 stent 并置,容器解剖和分支容器监狱的差别。在在严重地钙化的损害的 DES 培植看起来是包括显著地更大的最后的 stent CSA 和更大的尖锐的腔获得的更多的功效以前, 30 天、 6 月的向率也不是 different.ConclusionsCutting 汽球 angioplasty,没有增加在操作和 MACE 率在期间的复杂并发症 6 月。 | Zhe TANG Jing BAI Shao-Ping SU Yu WANG Mo-Han LIU Qi-Cai BAI Jin-Wen TIAN Qiao XUE Lei GAO Chun-Xiu AN Xiao-Juan LIU | 2014 | Journal of Geriatric Cardiology2014，11，1： | 9 |
| 6 | Electrical stimulation of the vagus nerve protects against cerebral ischemic injury through an anti-inflammatory mechanism显示文摘Vagus nerve stimulation exerts protective effects against ischemic brain injury; however, the underlying mechanisms remain unclear. In this study, a rat model of focal cerebral ischemia was established using the occlusion method, and the right vagus nerve was given electrical stimulation(constant current of 0.5 m A; pulse width, 0.5 ms; frequency, 20 Hz; duration, 30 seconds; every 5 minutes for a total of 60 minutes) 30 minutes, 12 hours, and 1, 2, 3, 7 and 14 days after surgery. Electrical stimulation of the vagus nerve substantially reduced infarct volume, improved neurological function, and decreased the expression levels of tumor necrosis factor-α and interleukin-6 in rats with focal cerebral ischemia. The experimental findings indicate that the neuroprotective effect of vagus nerve stimulation following cerebral ischemia may be associated with the inhibition of tumor necrosis factor-α and interleukin-6 expression. | Yao-xian Xiang Wen-xin Wang Zhe Xue Lei Zhu Sheng-bao Wang Zheng-hui Sun | 2015 | Neural Regeneration Research2015，10，4： | 8 |
| 7 | Imatinib induces H2AX phosphorylation and apoptosis in chronic myelogenous leukemia cells in vitro via caspase-3/Mst1 pathway显示文摘目的: Histone H2AX 是新奇肿瘤 suppressor,它在 C 终点(Ser139 和 Tyr142 ) 的 phosphorylation 为肿瘤房间 apoptosis 被要求。现在的学习的目的是阐明在 vitro 在长期的骨髓内产生的白血病房间位于 H2AX 的导致 imatinib 的 C 终端 phosphorylation 下面的机制。 | Yan-jun ZHANG Cheng-rong LU Yan CAO Yuan LUO Rong-feng BAO Shu YAN Mei XUE Feng ZHU Zhe WANG Lian-ning DUAN | 2012 | Acta Pharmacologica Sinica2012，33，4： | 8 |
| 8 | Whole genome sequencing of 10K patients with acute ischaemic stroke or transient ischaemic attack: design, methods and baseline patient characteristics显示文摘Background and purpose Stroke is the second leading cause of death worldwide and the leading cause of mortality and long-term disability in China,but its underlying risk genes and pathways are far from being comprehensively understood.We here describe the design and methods of whole genome sequencing(WGS)for 10914 patients with acute ischaemic stroke or transient ischaemic attack from the Third China National Stroke Registry(CNSR-III).Methods Baseline clinical characteristics of the included patients in this study were reported.DNA was extracted from white blood cells of participants.Libraries are constructed using qualified DNA,and WGS is conducted on BGISEQ-500 platform.The average depth is intended to be greater than 30×for each subject.Afterwards,Sentieon software is applied to process the sequencing data under the Genome Analysis Toolkit best practice guidance to call genotypes of single nucleotide variants(SNVs)and insertion-deletions.For each included subject,21 fingerprint SNVs are genotyped by MassARRAY assays to verify that DNA sample and sequencing data originate from the same individual.The copy number variations and structural variations are also called for each patient.All of the genetic variants are annotated and predicted by bioinformatics software or by reviewing public databases.Results The average age of the included 10914 patients was 62.2±11.3 years,and 31.4%patients were women.Most of the baseline clinical characteristics of the 10914 and the excluded patients were balanced.Conclusions The WGS data together with abundant clinical and imaging data of CNSR-III could provide opportunity to elucidate the molecular mechanisms and discover novel therapeutic targets for stroke. | Si Cheng Zhe Xu Yang Liu Jinxi Lin Yong Jiang Yilong Wang Xia Meng Anxin Wang Xinying Huang Zhimin Wang Guohua Chen Songdi Wu Zhengchang Jia Yongming Chen Xuerong Qiu Jun Wu Binbin Song Weizhong Ji Zhongping An Wenjun Xue Lili Zhao Yu Geng Hongyan Li Hao Li Yongjun Wang | 2021 | Stroke & Vascular Neurology2021，6，2： | 8 |
| 9 | CD147 antibody specifically and effectively inhibits infection and cytokine storm of SARS-CoV-2 and its variants delta,alpha,beta,and gamma显示文摘SARS-CoV-2 mutations contribute to increased viral transmissibility and immune escape,compromising the effectiveness of existing vaccines and neutralizing antibodies.An in-depth investigation on COVID-19 pathogenesis is urgently needed to develop a strategy against SARS-CoV-2 variants.Here,we identified CD147 as a universal receptor for SARS-CoV-2 and its variants.Meanwhile,Meplazeumab,a humanized anti-CD147 antibody,could block cellular entry of SARS-CoV-2 and its variants-alpha,beta,gamma,and delta,with inhibition rates of 68.7,75.7,52.1,52.1,and 62.3%at 60μg/ml,respectively.Furthermore,humanized CD147 transgenic mice were susceptible to SARS-CoV-2 and its two variants,alpha and beta.When infected,these mice developed exudative alveolar pneumonia,featured by immune responses involving alveoli-infiltrated macrophages,neutrophils,and lymphocytes and activation of IL-17 signaling pathway.Mechanistically,we proposed that severe COVID-19-related cytokine storm is induced by a'spike protein-CD147-CyPA signaling axis':Infection of SARS-CoV-2 through CD147 initiated the JAK-STAT pathway,which further induced expression of cyclophilin A(CyPA);CyPA reciprocally bound to CD147 and triggered MAPK pathway.Consequently,the MAPK pathway regulated the expression of cytokines and chemokines,which promoted the development of cytokine storm.Importantly,Meplazumab could effectively inhibit viral entry and inflammation caused by SARS-CoV-2 and its variants.Therefore,our findings provided a new perspective for severe COVID-19-related pathogenesis.Furthermore,the validated universal receptor for SARS-CoV-2 and its variants can be targeted for COVID-19 treatment. | Jiejie Geng Liang Chen Yufeng Yuan Ke Wang Youchun Wang Chuan Qin Guizhen Wu Ruo Chen Zheng Zhang Ding Wei Peng Du Jun Zhang Peng Lin Kui Zhang Yongqiang Deng Ke Xu Jiangning Liu Xiuxuan Sun Ting Guo Xu Yang Jiao Wu Jianli Jiang Ling Li Kun Zhang Zhe Wang Jing Zhang Qingguo Yan Hua Zhu Zhaohui Zheng Jinlin Miao Xianghui Fu Fengfan Yang Xiaochun Chen Hao Tang Yang Zhang Ying Shi Yumeng Zhu Zhuo Pei Fei Huo Xue Liang Yatao Wang Qingyi Wang Wen Xie Yirong Li Mingyan Shi Huijie Bian Ping Zhu Zhi-Nan Chen | 2021 | Signal Transduction and Targeted Therapy2021，6，10： | 6 |
| 10 | Xiaoyaosan improves depressive-like behaviors by regulating the NLRP3 signaling pathway in the rat cerebral cortex显示文摘Objective:To observe changes in the molecular expression of the NLR Family Pyrin Domain Containing Protein 3(NLRP3)pathway in depressed rats after treatment with Xiaoyaosan,and identify the regulatory mechanism of this compound.Methods:Male SpragueeD awley rats were randomly divided into five groups with 12 rats in each group,including the control group,model group,Fluoxetine group,Xiaoyaosan group,and MCC950 group.A depression model was generated by chronic immobilization stress(induced by 3 h of restraint immobilization every day),and the drugs were administered at the same time in each group for 21 days.The effects of Xiaoyaosan on behavioral changes of depressed rats were observed through macroscopic characterization,body mass,open field experiments,and a sucrose preference test.The m RNA and protein expression of the NLRP3 signaling pathway was examined by fluorescence real-time quantitative PCR and Western blot assays.Results:The Xiaoyaosan group,Fluoxetine group,and MCC950 group rats showed improved depressive behavior and an increased weight of sucrose water consumption.The protein and mRNA expression levels of NLRP3,Caspase-1,and IL-1 b were also decreased in the Fluoxetine,Xiaoyaosan,and MCC950 groups.Conclusion:NLRP3,Caspase-1,and IL-1 b protein and mRNA expression levels were increased in the cortex of depressed rats,while Xiaoyaosan protected cortical tissue in these rats by decreasing NLRP3,Caspase-1,and IL-1 b protein and mRNA expression. | Cong Chen Rong Yu Zhe Xue Zhiyi Yan Qinlai Bian Yajing Hou Yunzhi Chen Yueyun Liu Jiaxu Chen | 2020 | Journal of Traditional Chinese Medical Sciences2020，7，3： | 5 |
| 11 | Metformin activates chaperone-mediated autophagy and improves disease pathologies in an Alzheimer disease mouse model显示文摘Chaperone-mediated autophagy(CMA)is a lysosome-dependent selective degradation pathway implicated in the pathogenesis of cancer and neurodegenerative diseases.However,the mechanisms that regulate CMA are not fully understood.Here,using unbiased drug screening approaches,we discover Metformin,a drug that is commonly the first medication prescribed for type 2 diabetes,can induce CMA.We delineate the mechanism of CMA induction by Metformin to be via activation of TAK1-IKKα/β signaling that leads to phosphorylation of Ser85 of the key mediator of CMA,Hsc70,and its activation.Notably,we find that amyloid-beta precursor protein(APP)is a CMA substrate and that it binds to Hsc70 in an IKKα/β-dependent manner.The inhibition of CMA-mediated degradation of APP enhances its cytotoxicity.Importantly,we find that in the APP/PS1 mouse model of Alzheimer's disease(AD),activation of CMA by Hsc70 overexpression or Metformin potently reduces the accumulated brain Aβplaque levels and reverses the molecular and behavioral AD phenotypes.Our study elucidates a novel mechanism of CMA regulation via Metformin-TAK1-IKKα/β-Hsc70 signaling and suggests Metformin as a new activator of CMA for diseases,such as AD,where such therapeutic intervention could be beneficial. | Xiaoyan Xu Yaqin Sun Xufeng Cen Bing Shan Qingwei Zhao Tingxue Xie Zhe Wang Tingjun Hou Yu Xue Mengmeng Zhang Di Peng Qiming Sun Cong Yi Ayaz Najafov Hongguang Xia | 2021 | Protein & Cell2021，12，10： | 5 |
| 12 | Molecular Fingerprints of Soil Organic Matter in a Typical Freshwater Wetland in Northeast China显示文摘Natural wetlands are known to store huge amounts of organic carbon in their soils. Despite the importance of this storage,uncertainties remain about the molecular characteristics of soil organic matter(SOM), a key factor governing the stability of soil organic carbon(SOC). In this study, the molecular fingerprints of SOM in a typical freshwater wetland in Northeast China were investigated using pyrolysis gas-chromatography/mass-spectrometry technology(Py-GC/MS). Results indicated that the SOC, total nitrogen(TN),and total sulfur contents of the cores varied between 16.88% and 45.83%, 0.93% and 2.82%, and 1.09% and 3.79%, respectively. The bulk δ^13C and δ^15N varied over a range of 9.85‰, between –26.85‰ and –17.00‰, and between –0.126‰ and 1.002‰, respectively. A total of 134 different pyrolytic products were identified, and they were grouped into alkyl(including n-alkanes(C:0) and n-alkenes(C:1),aliphatics(Al), aromatics(Ar), lignin(Lg), nitrogen-containing compounds(Nc), polycyclic aromatic hydrocarbons(PAHs), phenols(Phs), polysaccharides(Ps), and sulfur-containing compounds(Sc). On average, Phs moieties accounted for roughly 24.11% peak areas of the total pyrolysis products, followed by Lg(19.27%), alkyl(18.96%), other aliphatics(12.39%), Nc compounds(8.08%), Ps(6.49%), aromatics(6.32%), Sc(3.26%), and PAHs(1.12%). Soil organic matter from wetlands had more Phs and Lg and less Nc moieties in pyrolytic products than soil organic matters from forests, lake sediments, pastures, and farmland.δ^13 C distribution patterns implied more C3 plant-derived soil organic matter, but the vegetation was in succession to C4 plant from C3 plant. Significant negative correlations between Lg or Ps proportions and C3 plant proportions were observed. Multiple linear analyses implied that the Ar and Al components had negative effects on SOC. Alkyl and Ar could facilitate ratios between SOC and total nitrogen(C/N), while Al plays the opposite role. Al was positively related to the ratio of dissolved organic carbon(DOC) to SOC. In summary, SOM of wetlands might characterize by more Phs and lignin and less Nc moieties in pyrolytic products. The use of Pyrolysis gas-chromatography/mass-spectrometry(Py-GC/MS) technology provided detailed information on the molecular characteristics of SOM from a typical freshwater wetland. | LI Zhe ZHANG Zhongsheng XUE Zhenshan SONG Xiaolin ZHANG Hongri WU Haitao JIANG Ming LYU Xianguo | 2019 | Chinese Geographical Science2019，29，4： | 4 |
| 13 | TMN4 complex embedded graphene as bifunctional electrocatalysts for high efficiency OER/ORR显示文摘Developing highly active bifunctional electrocatalysts for oxygen evolution reaction(OER)and oxygen reduction reaction(ORR)is of great significance in energy conversion and storage technologies.In this study,we systematically investigated the OER/ORR electrocatalytic activity of TMN_(4)@G system by using density functional theory(DFT)calculations.Globally,IrN_(4)@G is a very promising bifunctional catalyst for both OER and ORR with the extremely low overpotentials of 0.30 and 0.26 V,respectively.Such outstanding electrocatalytic performance is mainly attributed to the synergistic effect of Ir and N.More importantly,by constructing 2D activity volcano plots,we obtained the limiting overpotentials of TMN_(4)@G system with the values of 0.26 V for OER and 0.24 V for ORR.These findings open up new opportunities for further exploring graphene-based materials for highly efficient OER/ORR electrocatalysts. | Zhe Xue Xinyu Zhang Jiaqian Qin Riping Liu | 2021 | Journal of Energy Chemistry2021，30，4： | 3 |
| 14 | SIZ1-Mediated SUMOylation of ROS1 Enhances Its Stability and Positively Regulates Active DNA Demethylation in Arabidopsis显示文摘The 5-methylcytosine DNA glycosylase/lyase REPRESSOR OF SILENCING 1(ROS1)-mediated active DNA demethylation is critical for shaping the genomic DNA methylation landscape in Arabidopsis.Whether and how the stability of ROS1 may be regulated by post-translational modifications is unknown.Using a methylation-sensitive PCR(CHOP-PCR)-based forward genetic screen forArabidopsis DNA hyper-methyl-ation mutants,we identified the SUMO E3 ligase SIZ1 as a critical regulator of active DNA demethylation.Dysfunction of SIZ1 leads to hyper-methylation at approximately 1000 genomic regions.SIZ1 physically in-teracts with ROS1 and mediates the SUMOylation of ROS1.The SUMOylation of ROS1 is reduced in siz1 mutant plants.Compared with that in wild-type plants,the protein level of ROS1 is significantly decreased,whereas there is an increased level of ROS1 transcripts in siz1 mutant plants.Our results suggest that SIZ1-mediated SUMOylation of ROS1 promotes its stability and positively regulates active DNA demethylation. | Xiangfeng Kong Yechun Hong Yi-Feng Hsu Huan Huang Xue Liu Zhe Song Jian-Kang Zhu | 2020 | Molecular Plant2020，13，12： | 3 |
| 15 | Oxidation of CO on Nanometer Metal Oxides at Low Temperatures显示文摘OxidationofCOonNanometerMetalOxidesatLowTemperatures*ZHANGWen-xiang**,JIAMing-jun,ZHAOJing-zhe,ZHANGChun-lei,WUTong-hao(Depar... | ZHANG Wen xiang ** , JIA Ming jun, ZHAO Jing zhe, ZHANG Chun lei, WU Tong hao (Department of Chemistry, Jilin University, Changchun, 130023) WANG Xue guang (Fushun Research Institute of Petroleum and Petrochemicals, Fushun, 113001) SUN Tie | 1997 | Chemical Research in Chinese Universities1997，13，4： | 3 |
| 16 | Thermal expansion behavior of a β-LiAlSiO_4/Cu composite显示文摘A copper matrix composite reinforced by β-LiAlSiO4 with negative thermal expansion coefficient was fabricated using vacuum hot-pressed sintering technique. The thermal expansion behavior of the composite was investigated, and the average residual stress in the matrix was analyzed by a simple model. The results indicate that the residual stress in the matrix affects the thermal expansion properties. After heat treatment, the coefficient of thermal expansion (CTE) of the composite decreases greatly. The CTE of the composite after thermal cycling between 50-350°C is the lowest. | WANG Lidong XUE Zongwei LIU Zhe FEI Weidong | 2009 | Rare Metals2009，28，1： | 3 |
| 17 | Characteristics of Long-term Nonprogressors and Viremia Controllers Infected with HIV-1 via Contaminated Blood Donations or Transfusions Conducted 20 Years Earlier显示文摘To characterize long‐term nonprogressors(LTNPs)and viremia controllers(VCs),infected with HIV‐1 through contaminated blood donation or transfusion between 1992 and 1996 in Henan,China.LTNPs and VCs were defined by CD4+T lymphocyte(CD4)count and viral load(VL).Of29,294 patients infected with HIV‐1 via contaminated blood donation or transfusion that had conducted for more than 20 years,92 were LTNPs/VCs.There were 70 LTNPs(0.24%),43 | LIU Jia FAN Pan Ying XUE Xiu Juan YAN Jiang Zhou SUN Guo Qing LIU Chun Hua TIAN Sui An LI Ning SUN Ding Yong ZHU Qian WANG Zhe | 2017 | Biomedical and Environmental Sciences2017，30，12： | 3 |
| 18 | Can arbuscular mycorrhiza and fertilizer management reduce phosphorus runoff from paddy fields?显示文摘Our study sought to assess how much phosphorus(P) runoff from paddy fields could be cut down by fertilizer management and inoculation with arbuscular mycorrhizal fungi. A field experiment was conducted in Lalin River basin, in the northeast China: six nitrogen–phosphorus–potassium fertilizer levels were provided(0, 20%, 40%, 60%, 80%, and 100% of the recommended fertilizer supply), with or without inoculation with Glomus mosseae. The volume and concentrations of particle P(PP) and dissolved P(DP) were measured for each runoff during the rice growing season. It was found that the seasonal P runoff, including DP and PP, under the local fertilization was 3.7 kg/ha, with PP, rather than DP, being the main form of P in runoff water. Additionally, the seasonal P runoff dropped only by 8.9% when fertilization decreased by 20%; rice yields decreased with declining fertilization. We also found that inoculation increased rice yields and decreased P runoff at each fertilizer level and these effects were lower under higher fertilization. Conclusively, while rice yields were guaranteed arbuscular mycorrhizal inoculation and fertilizer management would play a key role in reducing P runoff from paddy fields. | Shujuan Zhang Li Wang Fang Ma Xue Zhang Zhe Li Shiyang Li Xiaofeng Jiang | 2015 | Journal of Environmental Sciences2015，27，7： | 3 |
| 19 | Cytochrome b5 reductase 2 suppresses tumor formation in nasopharyngeal carcinoma by attenuating angiogenesis显示文摘Background:Cytochrome b5 reductase 2(CYB5R2) is a potential tumor suppressor that inhibits cell proliferation and motility in nasopharyngeal carcinoma(NPC).Inactivation of CYB5R2 is associated with lymph node metastasis in NPC.This study aimed to explore the mechanisms contributing to the anti-neoplastic effects of CYB5R2.Methods:Polymerase chain reaction(PCR) assays were used to analyze the transcription of 84 genes known to be involved in representative cancer pathways in the NPC cell line HONE1.NPC cell lines CNE2 and HONE1 were transiently transfected with CYB5R2,and data was validated by real-time PCR.A chick chorioallantoic membrane(CAM)embryo model was implanted with CYB5R2-expressing CNE2 and HONE1 cells to evaluate the effect of CYB5R2 on angiogenesis.An immunohistochemical assay of the CAM model was used to analyze the protein expression of vascular endothelial growth factor(VEGF).Results:In CYB5R2-transfected NPC cells,PCR assays revealed up-regulated mRNA levels of Fas cell surface death receptor(FAS),FBJ murine osteosarcoma viral oncogene homolog(FOS),phosphoinositide-3-kinase regulatory subunit 1(PIK3R1),integrin beta 3(ITGB3),metastasis suppressor 1(MTSSl),interferon beta 1(IFNB1),and cyclin-dependent kinase inhibitor 2A(CDKN2A) and down-regulated levels of integrin beta 5(ITGB5),insulin-like growth factor 1(IGF1),TEK tyrosine kinase(TEK),transforming growth factor beta receptor 1(TGFBRl),and VEGF.The angiogenesis in the CAM model implanted with CYB5R2-transfected NPC cells was inhibited.Down-regulation of VEGF by CYB5R2 in NPC cells was confirmed by immunohistochemical staining in the CAM model.Conclusion:CYB5R2 up-regulates the expression of genes that negatively modulate angiogenesis in NPC cells and down-regulates the expression of VEGF to reduce angiogenesis,thereby suppressing tumor formation. | Huixin Ming Ying Lan Feng He Xue Xiao Xiaoying Zhou Zhe Zhang Ping Li Guangwu Huang | 2015 | Chinese Journal of Cancer2015，34，10： | 3 |
| 20 | Involvement of acid-sensing ion channel 1a in gastric carcinoma cell migration and invasion显示文摘酸的微型环境,特别地酸察觉到的离子隧道 1a (ASIC1a ) ,被报导了象移植一样支持癌房间增长。在这研究,我们在胃的癌(GC ) 的移植和侵略上探索了 ASIC1a 的效果。ASIC1a 表示层次被 immunohistochemistry 从 16 个病人在配对的 GC 和邻近的正常纸巾检验。反向的抄写即时 PCR 和 immunoblotting 被进行与 ASIC1a 小发卡 RNA (shRNA ) 在 transfection 以后在 GC 房间线 AGS 估计 ASIC1a 表示层次。创伤愈合和 transwell 侵略试金被利用检测转移和侵略追随者 ASIC1a silencing。肿瘤形成被用来在 vivo 在 tumorigenicity 检测 ASIC1a 的角色。ASIC1a 表示水平在与非转移相比显示出手术后的转移的 GC 纸巾和非肿瘤纸巾是显著地更高的,这被发现。而且,有 shRNA 的 ASIC1a 的 silencing 显著地下面调整的 ASIC1a 表示和减少的 GC 房间移植和侵略。中等酸的细胞外的环境禁止了 GC 房间生存能力。而且, ASIC1a shRNA 在 vivo 引起了 tumorigenicity 的抑制。我们的学习是 attenuating 的第一份报告在 vitro 并且在由压制 ASIC1a 的 vivo 的 GC 的恶意的显型,并且建议一条新奇途径学习在 ASIC 和 GC 房间移植和侵略之间的关系。 | Xin Chen Xue Sun Zhe Wang Xiaojun Zhou Lu Xu Feng'e Li Xingding Zhang Ji'an Pan Lin Qi Haixin Qian Zhongqi Mao | 2018 | Acta Biochimica et Biophysica Sinica2018，50，5： | 3 |
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