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| 1 | The role of furin cleavage site in SARS-CoV-2 spike protein-mediated membrane fusion in the presence or absence of trypsin显示文摘Dear Editor,The rapid spread of SARS-CoV-2(also known as 2019-nCoV and HCoV-191),a novel lineage B betacoronavirus(βCoV),has caused a global pandemic of coronavirus disease(COVID-19).It has been speculated that RRAR,a unique furin-like cleavage site(FCS)in the spike protein(S),which is absent in other lineage BβCoVs,such as SARS-CoV,is responsible for its high infectivity and transmissibility. | Shuai Xia Qiaoshuai Lan Shan Su Xinling Wang Wei Xu Zezhong Liu Yun Zhu Qian Wang Lu Lu Shibo Jiang | 2020 | Signal Transduction and Targeted Therapy2020，5，1： | 8 |
| 2 | RBD-Fc-based COVID-19 vaccine candidate induces highly potent SARS-CoV-2 neutralizing antibody response显示文摘The pandemic of coronavirus disease 2019(COVID-19)caused by severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)has posed serious threats to global health and economy,thus calling for the development of safe and effective vaccines.The receptorbinding domain(RBD)in the spike protein of SARS-CoV-2 is responsible for its binding to angiotensin-converting enzyme 2(ACE2)receptor.It contains multiple dominant neutralizing epitopes and serves as an important antigen for the development of COVID-19 vaccines.Here,we showed that immunization of mice with a candidate subunit vaccine consisting of SARS-CoV-2 RBD and Fc fragment of human IgG,as an immunopotentiator,elicited high titer of RBD-specific antibodies with robust neutralizing activity against both pseudotyped and live SARS-CoV-2 infections.The mouse antisera could also effectively neutralize infection by pseudotyped SARS-CoV-2 with several natural mutations in RBD and the IgG extracted from the mouse antisera could also show neutralization against pseudotyped SARS-CoV and SARS-related coronavirus(SARSr-CoV).Vaccination of human ACE2 transgenic mice with RBD-Fc could effectively protect mice from the SARS-CoV-2 challenge.These results suggest that SARS-CoV-2 RBD-Fc has good potential to be further developed as an effective and broad-spectrum vaccine to prevent infection of the current SARS-CoV-2 and its mutants,as well as future emerging SARSr-CoVs and re-emerging SARS-CoV. | Zezhong Liu Wei Xu Shuai Xia Chenjian Gu Xinling Wang Qian Wang Jie Zhou Yanling Wu Xia Cai Di Qu Tianlei Ying Youhua Xie Lu Lu Zhenghong Yuan Shibo Jiang | 2020 | Signal Transduction and Targeted Therapy2020，5，1： | 7 |
| 3 | Altered Motor-Striatal Plasticity and Cortical Functioning in Patients with Schizophrenia显示文摘Patients with schizophrenia undergo changes in brain plasticity. In the present study, we characterized motor cortical-striatal plasticity in such patients. Compared with the potentiation following high-frequency repetitive transcranial magnetic stimulation in the control group, the patients demonstrated impaired plasticity of corticostriatal motor-evoked potentials recorded from hand muscles.Notably, the loss of cortical plasticity was correlated with impaired motor learning in a rotary pursuit task. Moreover,the loss of plasticity was correlated with the symptoms of schizophrenia. The results suggest that the progression of schizophrenia is accompanied by altered cortical plasticity and functioning. | Dongsheng Zhou Feng Pang Shiyan Liu Ying Shen Lingjiang Liu Zezhong Fang Chuang Wang Zhenyu Hu Ti-Fei Yuan | 2017 | Neuroscience Bulletin2017，33，3： | 5 |
| 4 | A novel STING agonist-adjuvanted pan-sarbecovirus vaccine elicits potent and durable neutralizing antibody and T cell responses in mice,rabbits and NHPs显示文摘The emergence of SARS-CoV-2 variants and potentially other highly pathogenic sarbecoviruses in the future highlights the need for pan-sarbecovirus vaccines.Here,we discovered a new STING agonist,CF501,and found that CF501-adjuvanted RBD-FC vaccine(CF501/RBD-FC)elicited significantly stronger neutralizing antibody(nAb)and T cell responses than Alum-and cGAMP-adjuvanted RBD-FC in mice.Vaccination of rabbits and rhesus macaques(nonhuman primates,NHPs)with CF501/RBD-FC elicited exceptionally potent nAb responses against SARS-CoV-2 and its nine variants and 41 S-mutants,SARS-CoV and bat SARSr-CoVs.CF501/RBD-FC-immunized hACE2-transgenic mice were almost completely protected against SARS-CoV-2 challenge,even 6 months after the initial immunization.NHPs immunized with a single dose of CF501/RBD-FC produced high titers of nAbs.The immunized macaques also exhibited durable humoral and cellular immune responses and showed remarkably reduced viral load in the upper and lower airways upon SARS-CoV-2 challenge even at 108 days post the final immunization.Thus,CF501/RBD-Fc can be further developed as a novel pan-sarbecovirus vaccine to combat current and future outbreaks of sarbecovirus diseases. | Zezhong Liu Jie Zhou Wei Xu Wei Deng Yanqun Wang Meiyu Wang Qian Wang Ming Hsieh Jingming Dong Xinling Wang Weijin Huang Lixiao Xing Miaoling He Chunlin Tao Youhua Xie Yilong Zhang Youchun Wang Jincun Zhao Zhenghong Yuan Chuan Qin Shibo Jiang Lu Lu | 2022 | Cell Research2022，32，3： | 5 |
| 5 | A highly potent and stable pan-coronavirus fusion inhibitor as a candidate prophylactic and therapeutic for COVID-19 and other coronavirus diseases显示文摘The development of broad-spectrum antivirals against human coronaviruses(HCoVs)is critical to combat the current coronavirus disease 2019(COVID-19)pandemic caused by severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)and its variants,as well as future outbreaks of emerging CoVs.We have previously identified a polyethylene glycol-conjugated(PEGylated)lipopeptide,EK1C4,with potent pan-CoV fusion inhibitory activity.However,PEG linkers in peptide or protein drugs may reduce stability or induce anti-PEG antibodies in vivo.Therefore,we herein report the design and synthesis of a series of dePEGylated lipopeptide-based pan-CoV fusion inhibitors featuring the replacement of the PEG linker with amino acids in the heptad repeat 2 C-terminal fragment(HR2-CF)of HCoV-OC43.Among these lipopeptides,EKL1C showed the most potent inhibitory activity against infection by SARS-CoV-2 and its spike(S)mutants,as well as other HCoVs and some bat SARS-related coronaviruses(SARSr-CoVs)tested.The dePEGylated lipopeptide EKL1C exhibited significantly stronger resistance to proteolytic enzymes,better metabolic stability in mouse serum,higher thermostability than the PEGylated lipopeptide EK1C4,suggesting that EKL1C could be further developed as a candidate prophylactic and therapeutic for COVID-19 and other coronavirus diseases. | Jie Zhou Wei Xu Zezhong Liu Chao Wang Shuai Xia Qiaoshuai Lan Yanxing Cai Shan Su Jing Pu Lixiao Xing Youhua Xie Lu Lu Shibo Jiang Qian Wang | 2022 | Acta Pharmaceutica Sinica B2022，12，4： | 5 |
| 6 | Retraction Note to:SARS-CoV-2 infects T lymphocytes through its spike protein-mediated membrane fusion显示文摘The authors have retracted this article1.After the publication of this article,it came to the authors attention that in order to support the conclusions of the study,the authors should have used primary T cells instead of T-cell lines.In addition,there are concerns that the flow cytometry methodology applied here was flawed.These points resulted in the conclusions being considered invalid. | Xinling Wang Wei Xu Gaowei Hu Shuai Xia Zhiping Sun Zezhong Liu Youhua Xie Rong Zhang Shibo Jiang Lu Lu | 2020 | Cellular & Molecular Immunology2020，17，8： | 5 |
| 7 | Petrology,Geochemistry,and Sr-Nd-S Isotopic Compositions of the Ore-Hosting Biotite Monzodiorite in the Luanjiahe Gold Deposit,Jiaodong Peninsula,China显示文摘The Jiaodong Peninsula is one of the most important Au ore provinces in China.There is an ongoing debate on the correlation between ore formation and magmatism in this province,because few intrusive rocks exhibit a clear association with ore deposits.A mineralized biotite monzodiorite(BM)stock,with disseminated ore,pervasive phyllic alteration,and no deformation,was found in a borehole in the footwall of the Zhaoping fault within the Luanjiahe Au deposit,which may shed light on this debate.The biotite monzodiorite contains explosion breccias,miarolitic cavities,skeletal and den-dritic quartz,and late-stage evolved aplite dikes,and the in-situδ34S values of the disseminated pyrite which is associated with Au mineralization are-1.7‰ to 7.3‰(mean=3.5‰),indicative of a magmatic-hydrothermal system.These findings,combined with the reported age of 123 Ma,show that the intru-sion has close spatial,temporal,and geochemical relationships with Au mineralization in the area.The biotite monzodiorite is metaluminous,high-K calc-alkaline and shoshonitic,with enrichment in light rare earth elements(REEs)and large-ion lithophile elements(LILEs),depletion in high-field-strength elements(HFSEs),and enriched Sr-Nd isotopic compositions.The intrusion may be the product of par-tial melting of enriched lithospheric mantle with a small lower crustal component.The hydrous,Au-bearing,enriched mantle source,and the strongly oxidized magma that was generated,created fa-vorable conditions for Au mineralization. | Xiaofeng Yao Zhizhong Cheng Zezhong Du Zhenshan Pang Yuquan Yang Kun Liu | 2021 | Journal of Earth Science2021，32，1： | 4 |
| 8 | A pan-sarbecovirus vaccine induces highly potent and durable neutralizing antibody responses in non-human primates against SARS-CoV-2 Omicron variant显示文摘Dear Editor,Since the outbreak of Coronavirus Disease 2019(COVID-19)caused by severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)infection,numerous SARS-CoV-2 variants of concern(VOCs),such as Alpha(B.1.1.7),Beta(B.1.351),Gamma(P.1),and Delta(B.1.617.2),have emerged.Recently,the newly identified SARS-CoV-2 VOC,Omicron(B.1.1.529),has rapidly spread in many countries1 and is expected to replace Delta as the dominant variant circulating in the world.Compared to other VOCs,Omicron contains at least 32 mutations in spike(S)protein,including 15 mutations in the receptor-binding domain(RBD),and these mutations are known to confer resistance to neutralizing antibodies(nAbs)and sera of convalescent patients and people who have received COVID-19 vaccines.2–5 Therefore,vaccines able to induce potent and durable neutralizing antibodies against Omicron and other VOCs are urgently needed. | Zezhong Liu Jasper Fuk-Woo Chan Jie Zhou Meiyu Wang Qian Wang Guangxu Zhang Wei Xu Kenn Ka-Heng Chik Yilong Zhang Youchun Wang Kwok-Yung Yuen Lu Lu Shibo Jiang | 2022 | Cell Research2022，32，5： | 4 |
| 9 | Nitrogen partitioning and microbial protein synthesis in lactating dairy cows with different phenotypic residual feed intake显示文摘Background: Residual feed intake(RFI) is an inheritable measure of feed efficiency that is independent on level of production. However, physiological and metabolic mechanisms underlying divergent RFI are not fully elucidated.This study was conducted to investigate dietary nitrogen(N) partitioning and microbial protein synthesis in lactating dairy cows divergent in phenotypic RFI.Results: Thirty Holstein dairy cows(milk yield = 35.3 ± 4.71 kg/d;milk protein yield = 1.18 ± 0.13 kg/d;mean ± standard deviation) were selected for the experiment to derive RFI. After the RFI measurement period of 50 d, the 10 lowest RFI cows and 8 highest RFI cows were selected. The low RFI cows had lower dry matter intake(DMI, P < 0.05) than the high RFI cows, but they produced similar energy-corrected milk. The ratios of milk to DMI(1.41 vs. 1.24, P < 0.01) and energy-corrected milk to DMI(1.48 vs. 1.36, P < 0.01) were greater in low RFI cows than those in the high RFI cows. The low RFI cows had lower milk urea nitrogen than that in the high RFI cows(P = 0.05). Apparent digestibility of nutrients did not differ between two groups(P > 0.10). Compared with high RFI animals, the low RFI cows had a lower retention of N(5.72 vs. 51.4 g/d, P < 0.05) and a higher partition of feed N to milk N(29.7% vs. 26.5%, P < 0.05).Conclusions: The results suggest that differences in N partition, synthesis of microbial protein, and utilization of metabolizable protein could be part of the mechanisms associated with variance in the RFI. | Yunyi Xie Zezhong Wu Diming Wang Jianxin Liu | 2019 | Journal of Animal Science and Biotechnology2019，10，4： | 4 |
| 10 | Inefficiency of Sera from Mice Treated with Pseudotyped SARS-CoV to Neutralize 2019-nCoV Infection显示文摘Dear Editor,An outbreak of unusual pneumonia in Wuhan, China recently was caused by infection of a novel type of coronavirus. The virus and disease were denoted as 2019-nCoV and COVID-19, respectively, by the World Health Organization(WHO). Most recently, 2019-nCoV was renamed SARS-CoV-2 by Coronaviridae Study Group(CSG) of the International Committee on Taxonomy of Viruses(ICTV)(Gorbalenya et al. 2020)。 | Zezhong Liu Shuai Xia Xinling Wang Qiaoshuai Lan Wei Xu Qian Wang Shibo Jiang Lu Lu | 2020 | Virologica Sinica2020，35，3： | 3 |
| 11 | Repurposing of a clinically used anti-HPV agent to prevent and treat SARS-CoV-2 infection as an intranasal formulation显示文摘Dear Editor,Fan et al.have shown that whey protein,such as that found in human,bovine,and goat milk,could effectively inhibit SARS-CoV-2 infection,but they did not identify the components in whey protein having this activity.1 Since p-lactoglobulin(P-LG)is the main component of whey protein,we herein tested the anti-SARS-CoV-2 activity of p-LG and 3-hydroxyphthalic anhydride(3HP)-modified P-LG(3HP-P-LG),an active component in anti-human papillomavirus(HPV)biological dressing(JB01-BD)that has been used in clinics since 2013 to block cervical infection of HPV. | Chen Hua Qinhai Ma Yun Zhu Shuai Xia Zezhong Liu Lin Li Lu Lu Nanshan Zhong Shuwen Liu Zifeng Yang Shibo Jiang | 2021 | Signal Transduction and Targeted Therapy2021，6，9： | 1 |
| 12 | Analysis of Paleoseismic Events of Dahuzhuang Trench at the Xiadian Fault显示文摘The Xiadian Fault is a very important concealed active fault in the Beijing Plain. It is the seismogenic fault of the Sanhe-Pinggu MS8. 0 earthquake in 1679. The ancient earthquake sequence in the long historical period is of great significance to understand accurately the activity characteristics of the fault and effectively reduce the earthquake disaster risk in Beijing. We have re-interpreted the Dahuzhuang trench,and identified three layers of buried paleosol,six collapsed wedges and one sand liquefaction event. Further,through the comparison with the landmark strata and paleo-earthquake events revealed by other trenches on the fault,an ancient earthquake sequence with a long historical period of the Xiadian Fault was established:since the 31 ka,the Xiadian Fault has 11 occurrences of earthquake events(including the 1679 earthquake),and the average recurrence interval is about 2. 8 ka. The paleo-seismic sequence also shows that there is an ancient earthquake cluster period from 25 ka to 15 ka,and there are 5 strong earthquakes in the cluster period.The average recurrence interval is about 2. 0 ka,which reflects the phase difference of the Xiadian Fault activity. | DENG Mei SHEN Jun LI Xi DAI Xunye LIU Zezhong LI Kechang JIAO Xuankai | 2019 | Earthquake Research in China2019，33，3： | 1 |
| 13 | Preparation of Titanium-based Polyoxometalatxiue-ionic Liquid Catalysts and Application in the Synthesis of Degradable Polyester显示文摘Three kinds of Keggin-type titanium-based polyoxometalate-ionic liquid catalysts were prepared,and the chemical structure,crystal structure and thermal stability of the three catalysts were analyzed and tested by infrared spectrometer,ultraviolet-visible spectrometer,X-ray diffractometer,and thermogravimetric analyzer,respectively.Afterwards,their performance in catalyzing the coupling reaction of ethylene carbonate(EC)and dimethyl succinate(DMSu)to synthesize polyethylene succinate(PES)was studied,and the effects of different metals on the catalytic performance and the mechanism of action were discussed.The optimal process parameters for the coupling reaction were explored.Finally,the chemical structure of PES was characterized by infrared spectrometer.The results show that under the same process parameters,the polyoxometalate-ionic liquid catalyst Bmim_(5)[Ti(H_(2)O)TiMo_(11)O_(39)]had the best catalytic effect.Under the optimal process parameters,Bmim_(5)[Ti(H_(2)O)TiMo_(11)O_(39)]had obvious inhibitory effect on side reactions,and the yield and selectivity of PES prepolymer were up to 72.23%and 88.64%. | Ran CHEN Changyuan YU Liying GUO Kun LIU Xiudan ZHAO Zezhong JIANG | 2022 | Meteorological and Environmental Research2022，13，4： | 1 |
| 14 | Double insult: flu bug enhances SARS-CoV-2 infectivity显示文摘The continuing COVID-19 pandemic and emergence of SARS-CoV-2 variants may cause this disease to transit into endemicity like seasonal flu.In a recent study,Bai et al.reported that preinfection of influenza A virus enhanced SARS-CoV-2 infectivity,calling for the development of a pan-flu-COVID-19 vaccine to combat infection of IAV,SARS-CoV-2,or their coinfection.The COVID-19 pandemic,caused by SARS-CoV-2,may become even more serious by the emergence of variants with altered transmissibility,pathogenesis,and virulence,or a combination,under evolutionary pressure. | Shan Su Zezhong Liu Shibo Jiang | 2021 | Cell Research2021，31，5： | 1 |
| 15 | The putative mechanism of lymphopenia in CoVID-19 patients显示文摘Severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)and its variants have caused>5oo million confirmed cases and>6 million deaths.Apart from the common clinical manifestations,63% of admitted coronavirus disease 2019(COvID-19)patients had lymphopenia,increasingto 85% in patients with severe disease(Huang et al.,2020). | Xinling Wang Zezhong Liu Lu Lu Shibo Jiang | 2022 | Journal of Molecular Cell Biology2022，14，5： | 1 |
| 16 | An ultrapotent pan-β-coronavirus lineage B(β-CoV-B)neutralizing antibody locks the receptor-binding domain in closed conformation by targeting its conserved epitope显示文摘New threats posed by the emerging circulating variants of SARS-CoV-2 highlight the need to find conserved neutralizing epitopes for therapeutic antibodies and efficient vaccine design.Here,we identified a receptorbinding domain(RBD)-binding antibody,XG014,which potently neutralizesβ-coronavirus lineage B(β-CoV-B),including SARS-CoV-2,its circulating variants,SARSCoV and bat SARSr-CoV WIV1.Interestingly,antibody family members competing with XG014 binding show reduced levels of cross-reactivity and induce antibodydependent SARS-CoV-2 spike(S)protein-mediated cellcell fusion,suggesting a unique mode of recognition by XG014.Structural analyses reveal that XG014 recognizes a conserved epitope outside the ACE2 binding site and completely locks RBD in the non-functional“down”conformation,while its family member XG005 directly competes with ACE2 binding and position the RBD“up”.Single administration of XG014 is effective in protection against and therapy of SARS-CoV-2 infection in vivo.Our findings suggest the potential to develop XG014 as pan-β-CoV-B therapeutics and the importance of the XG014 conserved antigenic epitope for designing broadly protective vaccines againstβ-CoV-B and newly emerging SARS-CoV-2 variants of concern. | Zezhong Liu Wei Xu Zhenguo Chen Wangjun Fu Wuqiang Zhan Yidan Gao Jie Zhou Yunjiao Zhou Jianbo Wu Qian Wang Xiang Zhang Aihua Hao Wei Wu Qianqian Zhang Yaming Li Kaiyue Fan Ruihong Chen Qiaochu Jiang Christian TMayer Till Schoofs Youhua Xie Shibo Jiang Yumei Wen Zhenghong Yuan Kang Wang Lu Lu Lei Sun Qiao Wang | 2022 | Protein & Cell2022，13，9： | 1 |
| 17 | Simplified parasitic capacitance extraction of shield in HVDC converter system with boundary element method显示文摘It is critical to build a wide-band circuit model to conduct research on the characteristics of the electromagnetic disturbance source during the localization of high voltage direct current(HVDC)technology.Parasitic capacitance is most essential for modeling the equivalent circuit,so a fast and accurate computation of capacitance parameters plays a vital role.Because of the large size and complex structure of the converter equipment,it is impossible to obtain capacitance parameters by means of measurement or simulating calculation with finite element software.In this paper,a simplified method of capacitance extraction based on boundary element method is proposed,which can provide an efficient means of establishing simulation models.In the method presented,simulation model of the shield may not be chamfered.Consequently,the edge and corner of the shield do not need to be handled with a sphere,cylinder and other curved surface model.The availability of this method is demonstrated by comparing the capacitance parameters of chamfered shield with that of non-chamfered shield. | Shili LIU Zezhong WANG Jing SUN | 2010 | Frontiers of Electrical and Electronic Engineering in China2010，5，4： | 0 |
| 18 | RETRACTED ARTICLE:SARS-CoV-2 infects T lymphocytes through its spike protein-mediated membrane fusion显示文摘The authors have retracted this article [1]. After the publication ofthis article, it came to the authors attention that in order tosupport the conclusions of the study, the authors should haveused primary T cells instead of T cell lines. Additionally, there areconcerns that the flow cytometry methodology applied here wasflawed. These points resulted in the conclusions being consideredinvalid. | Xinling Wang Wei Xu Gaowei Hu Shuai Xia Zhiping Sun Zezhong Liu Youhua Xie Rong Zhang Shibo Jiang Lu Lu | 2023 | Cellular & Molecular Immunology2023，20，5： | 0 |
| 19 | Hand Rearing Common Zebra (Equus quagga)显示文摘This paper documented a successful hand rearing process of a 43-day-old zebra which was raised for 174 d and weaned at 217 days of age.The hand-rearing condition was recorded from nursing techniques,medical care and animal husbandry,focusing on formulas and supplements.Relevant issues of hand rearing were also discussed. | Liu He Wu Zhaozheng Zhang Jinguo Wang Zezhong Wang Yue | 2014 | Animal Husbandry and Feed Science2014，6，1： | 0 |
| 20 | Thermo-Mechanical Analysis of the Sealing Performance of a Diesel-Engine Cylinder Gasket显示文摘Taking the combustor composite structure of a high-strength diesel engine as the main research object,dedicated tests have been conducted to verify the accuracy of three distinct cylinder gasket pressure simulation models.Using the measured cylinder gasket compression rebound curve,a gasket unit has been designed and manufactured.For this unit,the influence of the bolt pretension,cylinder body and cylinder head material on gasket sealing pressure has been investigated systematically in conditions of thermo-mechanical coupling.The results show that the bolt pretension force is one of the most important factors affecting the cylinder gasket sealing pressure.The change of the body material has little effect on this pressure.The cylinder gasket seal pressure decreases progressively with the reduction of the elastic module of the cylinder head material. | Xulan Wang Zezhong Chen Changzhen Liu Wei Ren Bo Wu Shude Ji Xiaofei Chen Shijie Wen Yonggang Hao | 2021 | Fluid Dynamics & Materials Processing2021，17，4： | 0 |
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