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| 1 | Acute kidney injury in acute-on-chronic liver failure is different from in decompensated cirrhosis显示文摘AIM To evaluate the differences in acute kidney injury(AKI) between acute-on-chronic liver failure(ACLF) and decompensated cirrhosis(DC) patients. METHODS During the period from December 2015 to July 2017, 280 patients with hepatitis B virus(HBV)-related ACLF(HBV-ACLF) and 132 patients with HBV-related DC(HBV-DC) who were admitted to our center were recruited consecutively into an observational study. Urine specimens were collected from all subjects and the levels of five urinary tubular injury biomarkers were detected,including neutrophil gelatinase-associated lipocalin(NGAL), interleukin-18(IL-18), liver-type fatty acid binding protein(L-FABP), cystatin C(CysC), and kidney injury molecule-1(KIM-1). Simultaneously, the patient demographics, occurrence and progression of AKI, and response to terlipressin therapy were recorded. All patients were followed up for 3 mo or until death after enrollment. RESULTS AKI occurred in 71 and 28 of HBV-ACLF and HBV-DC patients, respectively(25.4% vs 21.2%, P = 0.358). Among all patients, the levels of four urinary biomarkers(NGAL, CysC, L-FABP, IL-18) were significantly elevated in patients with HBV-ACLF and AKI(ACLF-AKI), compared with that in patients with HBV-DC and AKI(DC-AKI) or those without AKI. There was a higher proportion of patients with AKI progression in ACLF-AKI patients than in DC-AKI patients(49.3% vs 17.9%, P = 0.013). Fortythree patients with ACLF-AKI and 19 patients with DC-AKI were treated with terlipressin. The response rate of ACLFAKI patients was significantly lower than that of patients with DC-AKI(32.6% vs 57.9%, P = 0.018). Furthermore, patients with ACLF-AKI had the lowest 90 d survival rates among all groups(P < 0.001).CONCLUSION AKI in ACLF patients is more likely associated with structural kidney injury, and is more progressive, with a poorer response to terlipressin treatment and a worse prognosis than that in DC patients. | Qun-Qun Jiang Mei-Fang Han Ke Ma Guang Chen Xiao-Yang Wan Semvua Bukheti Kilonzo Wen-Yu Wu Yong-Li Wang Jie You Qin Ning | 2018 | World Journal of Gastroenterology2018，24，21： | 25 |
| 2 | Cystatin C is a biomarker for predicting acute kidney injury in patients with acute-on-chronic liver failure显示文摘AIM:To investigate serum cystatin C level as an early biomarker for predicting acute kidney injury(AKI)in patients with acute-on-chronic liver failure(ACLF).METHODS:Fifty-six consecutive patients with hepatitis B virus-related ACLF who had normal serum creatinine(Cr)level(<1.2 mg/dL in men,or<1.1 mg/dL in women)were enrolled in the Liver Failure Treatment and Research Center of Beijing 302 Hospital between August 2011 and October 2012.Thirty patients with chronic hepatitis B(CHB)and 30 healthy controls in the same study period were also included.Measurement of serum cystatin C(CysC)was performed by a particle-enhanced immunonephelometry assay using the BN Prospec nephelometer system.The ACLF patients were followed during their hospitalization period.RESULTS:In the ACLF group,serum level of CysC was 1.1±0.4 mg/L,which was significantly higher(P<0.01)than those in the healthy controls(0.6±0.3mg/L)and CHB patients(0.7±0.2 mg/L).During the hospitalization period,eight ACLF patients developed AKI.Logistic regression analysis indicated that CysC level was an independent risk factor for AKI development(odds ratio=1.8;95%CI:1.4-2.3,P=0.021).The cutoff value of serum CysC for prediction of AKI in ACLF patients was 1.21 mg/L.The baseline CysC-based estimated glomerular filtration rate(eGFR CysC)was significantly lower than the creatinine-based eGFR(eGFR CG and eGFR MDRD)in ACLF patients with AKI,suggesting that baseline eGFR CysC represented early renal function in ACLF patients while the Cr levels were still within the normal ranges.CONCLUSION:Serum CysC provides early prediction of renal dysfunction in ACLF patients with a normal serum Cr level. | Zhi-Hong Wan Jian-Jun Wang Shao-Li You Hong-Ling Liu Bing Zhu Hong Zang Chen Li Jing Chen Shao-Jie Xin | 2013 | World Journal of Gastroenterology2013，19，48： | 20 |
| 3 | Formaldehyde up-regulates TRPV1 through MAPK and PI3K signaling pathways in a rat model of bone cancer pain显示文摘Objective Our previous study showed that tumor tissue-derived formaldehyde at low concentrations plays an important role in bone cancer pain through activating transient receptor potential vanilloid subfamily member 1 (TRPV1). The present study further explored whether this tumor tissue-derived endogenous formaldehyde regulates TRPV1 expression in a rat model of bone cancer pain, and if so, what the possible signal pathways are during the development of this type of pain. Methods A rat model of bone cancer pain was established by injecting living MRMT-1 tumor cells into the tibia. The formaldehyde levels were determined by high performance liquid chromatography, and the expression of TRPV1 was examined with Western blot and RT-PCR. In primary cultured dorsal root ganglion (DRG) neurons, the expression of TRPV1 was assessed after treatment with 100 μmol/L formaldehyde with or without pre-addition of PD98059 [an inhibitor for extracellular signal-regulated kinase], SB203580 (a p38 inhibitor), SP600125 [an inhibitor for c-Jun N-terminal kinase], BIM [a protein kinase C (PKC) inhibitor] or LY294002 [a phosphatidylinositol 3-kinase (PI3K) inhibitor]. Results In the rat model of bone cancer pain, formaldehyde concentration increased in blood plasma, bone marrow and the spinal cord. TRPV1 protein expression was also increased in the DRG. In primary cultured DRG neurons, 100 μmol/L formaldehyde significantly increased the TRPV1 expression level. Pre-incubation with PD98059, SB203580, SP600125 or LY294002, but not BIM, inhibited the formaldehyde-induced increase of TRPV1 expression. Conclusion Formaldehyde at a very low concentration up-regulates TRPV1 expression through mitogen-activated protein kinase and PI3K, but not PKC, signaling pathways. These results further support our previous finding that TRPV1 in peripheral afferents plays a role in bone cancer pain. | Ying Han Yan Li Xlng Xlao Jia Liu Xiang-Ling Meng Feng-Yu Liu Guo-Gang Xing You Wan | 2012 | Neuroscience Bulletin2012，28，2： | 19 |
| 4 | Situation Awareness of Active Distribution Network:Roadmap,Technologies,and Bottlenecks显示文摘With the rapid development of local generation and demand response,the active distribution network(ADN),which aggregates and manages miscellaneous distributed resources,has moved from theory to practice.Secure and optimal operations now require an advanced situation awareness(SA)system so that operators are aware of operation states and potential risks.Current solutions in distribution supervisory control and data acquisition(DSCADA)as well as the distribution automation system(DAS)generally are not able to meet the technology requirements of SA.In this paper,the authors’participation in the project of developing an SA system as the basic component of a practical active distribution management system(ADMS)deployed in Beijing,China,is presented.This paper reviews the ADN’s development roadmap by illustrating the changes that are made in elements,topology,structure,and control scheme.Taking into consideration these hardware changes,a systematic framework is proposed for the main components and the functional hierarchy of an SA system for the ADN.The SA system’s implementation bottlenecks are also presented,including,but not limited to issues in big data platform,distribution forecasting,and security evaluation.Potential technology solutions are also provided. | Jin Lin Can Wan Yonghua Song Renle Huang Xiaoshuang Chen Wanfang Guo Yi Zong You Shi | 2016 | CSEE Journal of Power and Energy Systems2016，2，3： | 13 |
| 5 | Clinical characteristics and corticosteroid therapy in patients with autoimmune-hepatitis-induced liver failure显示文摘AIM:To investigate the clinical features,response to corticosteroids,and prognosis of autoimmune hepatitis(AIH)-induced liver failure in China.METHODS:A total of 22 patients(19 female and 3male;average age 51±15 years)with AIH-induced liver failure treated in our hospital from 2004 to 2012were retrospectively analyzed.Clinical,biochemical and pathological characteristics of the 22 patients and responses to corticosteroid treatment in seven patients were examined retrospectively.The patients were divided into survivor and non-survivor groups,and the clinical characteristics and prognosis were compared between the two groups.The t test was used for data analysis of all categorical variables,and overall survival was calculated by the Kaplan-Meier method.RESULTS:At the time of diagnosis,mean IgG was2473±983 mg/dL,with three(18.8%)patients showing normal levels.All of the patients had elevated serum levels of antinuclear antibody(≥1:640).Liver histology from one patient showed diagnostic pathological changes,including massive necrosis and plasma cell infiltration.Four patients survived(18.2%)and 18died(81.8%)without liver transplantation.The results showed that patients with low admission Model for End-Stage Liver Disease(MELD)scores(21.50±2.08vs 30.61±6.70,P<0.05)and corticosteroid therapy(100%vs 16.7%,P<0.05)had better prognosis.A total of seven patients received corticosteroid therapy,of whom,four responded and survived,and the other three died.Survivors showed young age,shorter duration from diagnosis to corticosteroid therapy,low MELD score,and absence of hepatic encephalopathy at the time of corticosteroid administration.Six patients who were administered corticosteroids acquired fungal infections but recovered after antifungal therapy.CONCLUSION:Early diagnosis and corticosteroid therapy are essential for improving the prognosis of patients with AIH-induced liver failure without liver transplantation. | Bing Zhu Shao-Li You Zhi-Hong Wan Hong-Ling Liu Yi-Hui Rong Hong Zang Shao-Jie Xin | 2014 | World Journal of Gastroenterology2014，20，23： | 10 |
| 6 | An experimental study of salt expansion in sodium saline soils under transient conditions显示文摘Salt expansion in sulfate saline soils that are widely distributed in northwestern China causes serious infrastructural damages under low-temperature conditions. However, the mechanism of salt expansion under low temperatures is not clear. In this study, we conducted a series of cooling experiments combined with salt crystallization to study this mechanism, and employed an ionic model to calculate the supersaturation ratio of the solution. During the experiments, the strength and the process of salt expansion were examined under different cooling rates and various crystal morphologies. The relationship between temperature and supersaturation ratio under transient conditions was also considered. Results indicate that the initial supersaturation ratio of a sodium sulfate solution is closely related to environmental conditions, and that this ratio decreases with slowing the cooling rates and stabilizing the crystal forms. Higher initial supersaturation ratios lead to an increased non-steady-state zone, resulting in less salt expansion. On the other hand, chloride ion content has a distinct influence on the crystallization supersaturation ratio of the sodium sulfate solution, and higher chloride ion content can inhibit salt expansion in sodium saline soils. These findings help explain salt expansion mechanisms in complex conditions such as seasonally frozen soils, and thus help search for improved methods of preventing salt expansion in sulfate saline soils. | WAN Xusheng YOU Zhemin WEN Haiyan William CROSSLEY | 2017 | Journal of Arid Land2017，9，6： | 9 |
| 7 | Involvement of hyperpolarization-activated,cyclic nucleotide-gated cation channels in dorsal root ganglion in neuropathic pain显示文摘Dorsal root ganglion(DRG)neurons have peripheral terminals in skin,muscle,and other peripheral tissues,andcentral | WAN You | 2008 | 生理学报2008，60，5： | 9 |
| 8 | Progress in ceramic materials and structure design toward advanced thermal barrier coatings显示文摘Thermal barrier coatings(TBCs)can effectively protect the alloy substrate of hot components in aeroengines or land-based gas turbines by the thermal insulation and corrosion/erosion resistance of the ceramic top coat.However,the continuous pursuit of a higher operating temperature leads to degradation,delamination,and premature failure of the top coat.Both new ceramic materials and new coating structures must be developed to meet the demand for future advanced TBC systems.In this paper,the latest progress of some new ceramic materials is first reviewed.Then,a comprehensive spalling mechanism of the ceramic top coat is summarized to understand the dependence of lifetime on various factors such as oxidation scale growth,ceramic sintering,erosion,and calcium–magnesium–aluminium–silicate(CMAS)molten salt corrosion.Finally,new structural design methods for high-performance TBCs are discussed from the perspectives of lamellar,columnar,and nanostructure inclusions.The latest developments of ceramic top coat will be presented in terms of material selection,structural design,and failure mechanism,and the comprehensive guidance will be provided for the development of next-generation advanced TBCs with higher temperature resistance,better thermal insulation,and longer lifetime. | Zhi-Yuan WEI Guo-Hui MENG Lin CHEN Guang-Rong LI Mei-Jun LIU Wei-Xu ZHANG Li-Na ZHAO Qiang ZHANG Xiao-Dong ZHANG Chun-Lei WAN Zhi-Xue QU Lin CHEN Jing FENG Ling LIU Hui DONG Ze-Bin BAO Xiao-Feng ZHAO Xiao-Feng ZHANG Lei GUO Liang WANG Bo CHENG Wei-Wei ZHANG Peng-Yun XU Guan-Jun YANG Hong-Neng CAI Hong CUI You WANG Fu-Xing YE Zhuang MA Wei PAN Min LIU Ke-Song ZHOU Chang-Jiu LI | 2022 | Journal of Advanced Ceramics2022，11，7： | 9 |
| 9 | Dislocations-induced precipitates and their effect on mechanical properties of Mg-Gd-Y-Zr alloy显示文摘Optical microscope(OM),scanning electron microscope(SEM),transmission electron microscope(TEM)and tensile machine were used to characterize the microstructures and mechanical properties of as-forged and aged Mg-9.5Gd-3.8Y-0.6Zr alloys.The results show that a novel kind of dislocation arrays,comprising parallel arranged dislocations,were obtained in the forged alloy.The arrays tend to extend parallel and are heterogeneously distributed with adjacent distances varying from 0.3μm to 1.4μm.After aging the alloy at 265°C,a large number of preferential-oriented phases were precipitated on the dislocation arrays,forming a structure of'precipitation chains'(PCs),which results in simultaneous increments of strength and ductility. | Chao You Churning Liu Yingchun Wan Bei Tang Bizheng Wang Yonghao Gao Xiuzhu Han | 2019 | Journal of Magnesium and Alloys2019，7，3： | 8 |
| 10 | A novel deletion mutation, c.1296delT in the BCOR gene, is associated with oculo-facio-cardio-dental syndrome显示文摘The purpose of the present study was to analyze the clinical phenotypes of a girl with oculo-facio-cardio-dental(OFCD)syndrome and to identify the potential pathogenic mutation responsible for her disease. The patient underwent detailed clinical examinations and phenotype data were collected over a follow-up period of 9 years. Mutation analysis of the candidate gene BCOR was performed with polymerase chain reaction and Sanger sequencing. BCOR of 60 unrelated normal individuals were also sequenced as a control group. Clinical phenotyping and follow-up study results indicate that this patient had multiple system anomalies including ocular, facial, cardiac, dental, and limb malformations. In addition, papilloma of the choroid plexus was identified, which represents the first report of this phenotype in an OFCD patient. A novel deletion mutation, c.1296 delT in exon4 of the BCOR gene, was identified in this patient and was not found in her parents or in 60 normal unrelated individuals. This deletion was a frameshift mutation and is proposed to encode a premature stop codon, thus producing a truncated protein. Our patient fitted the diagnostic criteria for OFCD syndrome and we report the first papilloma of the choroid plexus in an OFCD patient, expanding the recognized phenotypic spectrum of this disease. Meanwhile, we identified a novel deletion mutation that may cause OFCD syndrome. | Jingshang Zhang Hongyan Jia Jinda Wang Ying Xiong Jing Li Xiaoxia Li Jing Zhao Xiaohui Zhang Qisheng You Guyu Zhu Frank F.Tsai Mark Espina Xiuhua Wan | 2019 | Science China(Life Sciences)2019，62，1： | 6 |
| 11 | Elevated Neurosteroids in the Lateral Thalamus Relieve Neuropathic Pain in Rats with Spared Nerve Injury显示文摘Neurosteroids are synthesized in the nervous system from cholesterol or steroidal precursors imported from peripheral sources. These compounds are important allosteric modulators of c-aminobutyric acid A receptors(GABA_ARs), which play a vital role in pain modulation in the lateral thalamus, a main gate where somatosensory information enters the cerebral cortex. Using high-performance liquid chromatography/tandem mass spectrometry,we found increased levels of neurosteroids(pregnenolone,progesterone, deoxycorticosterone, allopregnanolone, and tetrahydrodeoxycorticosterone) in the chronic stage of neuropathic pain(28 days after spared nerve injury) in rats.The expression of the translocator protein TSPO, the upstream steroidogenesis rate-limiting enzyme, increased at the same time. In vivo stereotaxic microinjection of neurosteroids or the TSPO activator AC-5216 into the lateral thalamus(AP-3.0 mm, ML ±3.0 mm, DV 6.0 mm)alleviated the mechanical allodynia in neuropathic pain,while the TSPO inhibitor PK 11195 exacerbated it. The analgesic effects of AC-5216 and neurosteroids were significantly attenuated by the GABA_AR antagonist bicuculline. These results suggested that elevated neurosteroids in the lateral thalamus play a protective role in the chronic stage of neuropathic pain. | Meng Zhang Jia Liu Meng-Meng Zhou Honghai Wu Yanning Hou Yun-Feng Li Yuxin Yin Lemin Zheng Feng-Yu Liu Ming Yi You Wan | 2016 | Neuroscience Bulletin2016，32，4： | 6 |
| 12 | A gate size estimation algorithm for data association filters显示文摘The problem of forming validation regions or gates for new sensor measurements obtained when tracking targets in clutter is considered. Since the gate size is an integral part of the data association filter, this paper is intended to describe a way of estimating the gate size via the performance of the data association filter. That is, the gate size can be estimated by looking for the optimal performance of the data association filter. Simulations show that this estimation method of the gate size offers advantages over the common and classical estimation methods of the gate size, especially in a heavy clutter and/or false alarm environment. | WANG MingHui WAN Qun YOU ZhiSheng | 2008 | Science in China(Series F)2008，51，4： | 6 |
| 13 | Immune dysfunction leads to mortality and organ injury in patients with COVID-19 in China:insights from ERS-COVID-19 study显示文摘Dear Editor,A series of novel coronavirus disease 2019(COVID-19)caused by severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)since the end of 2019 is ongoing and triggering a global public health crisis.The estimated case fatality rate is approximately 3.4%in China.However,some patients experience dyspnea within 1 week and develop rapidly to organ injury and even death within 2 weeks after dyspnea.1 In addition,early organ injury could lead to higher risks of mortality.Thus,early identification of patients at risk of organ injury and death is crucial,which saves the patients from classified and invasive treatment,improving clinical outcome and prognosis.The human immune system plays significant roles in the resistance of foreign pathogens and the progress of pneumonia.Recent studies have mentioned that T cells were decreased in COVID-19 patients,excessive activated immune response was caused by pathogenic Th1 cells,and inflammatory CD14+CD16+monocytes may connect to pulmonary immunopathology,leading to deleterious clinical manifestations and even acute mortality after SARS-CoV-2 infections.2 SARS-CoV-2 might damage lymphocytes,especially T lymphocytes,and the immune system was impaired during the period of disease to cause tissue injury. | Dongze Li You Chen Hong Liu Yu Jia Fanghui Li Wei Wang Jiang Wu Zhi Wan Yu Cao Rui Zeng | 2020 | Signal Transduction and Targeted Therapy2020，5，1： | 5 |
| 14 | Formaldehyde increases intracellular calcium concentration in primary cultured hippocampal neurons partly through NMDA receptors and T-type calcium channels显示文摘在高集中的客观甲醛是一个贡献者让污染通风。它也是在房间的一个内长的新陈代谢的产品,并且什么时候在生理的集中以外,在神经原上有病理学的效果。甲醛在癌症疼痛模型导致外部 nociceptive 神经原的记忆功能,以及刺激的 neuronal 字形物蛋白质,海马趾的 neuronal apoptosis,认知缺陷和损失的合拢 mis 和聚集。细胞内部的钙([Ca2+] 我) 是一个重要细胞内部的送信人,并且在许多病理学的过程起一个关键作用。现在的学习试图调查甲醛的效果在上[Ca2+] i 和 N-methyl-D-aspartate 受体(NMDAR ) 和房间膜上的 T 类型 Ca2+ 隧道的可能的参与。把主要有教养的海马趾的神经原用作一个模型,的方法变化[Ca2+] 我被共焦的激光扫描面对在低集中的甲醛检测显微镜学。在近似加倍的 1 mmol/L 的结果甲醛[Ca2+] 我。( 2R ) -amino-5-phosphonopentanoate ( AP5 , 25 mol/L ,一个 NMDAR 对手)并且 mibefradil ( MIB , 1 mol/L ,T类型Ca2+隧道 blocker ),在甲醛灌注以后给 5 min ,部分各禁止了导致甲醛的增加[Ca2+]我,并且这禁止的效果被 AP5 和 MIB 的联合申请增强。什么时候在甲醛灌注前使用了 3 min, AP5 (甚至在 50 mol/L ) 没禁止导致甲醛的增加[Ca2+] 我,而是 MIB (1 mol/L ) 显著地在 70% 禁止了这增加。这些结果在低集中建议那甲醛的结论增加[Ca2+] 在有教养的海马趾的神经原的 i;NMDAR 和 T 类型 Ca2+ 隧道可以涉及这个过程。 | Ye-Nan Chi Xu Zhang Jie Cai Feng-Yu Liu Guo-Gang Xing You Wan | 2012 | Neuroscience Bulletin2012，28，6： | 4 |
| 15 | A Context-Based Analgesia Model in Rats: Involvement of Prefrontal Cortex显示文摘Cognition and pain share common neural substrates and interact reciprocally: chronic pain compromises cognitive performance, whereas cognitive processes modulate pain perception. In the present study, we established a non-drug-dependent rat model of context-based analgesia,where two different contexts(dark and bright) were matched with a high(52°C) or low(48°C) temperature in the hot-plate test during training. Before and after training,we set the temperature to the high level in both contexts.Rats showed longer paw licking latencies in trials with the context originally matched to a low temperature than those to a high temperature, indicating successful establishment of a context-based analgesic effect in rats. This effect was blocked by intraperitoneal injection of naloxone(an opioid receptor antagonist) before the probe. The context-based analgesic effect also disappeared after optogenetic activation or inhibition of the bilateral infralimbic or prelimbic sub-region of the prefrontal cortex. In brief, we established a context-based, non-drug dependent, placebo-like analgesia model in the rat. This model provides a new and useful tool for investigating the cognitive modulation of pain. | Lingchi Xu Yalan Wan Longyu Ma Jie Zheng Bingxuan Han Feng-Yu Liu Ming Yi You Wan | 2018 | Neuroscience Bulletin2018，34，6： | 4 |
| 16 | Peginterferon Alfa-2a Plus Ribavirin for the Treatment of Dual Chronic Infection With Hepatitis B and C Viruses显示文摘 | Chun–Jen Liu Wan–Long Chuang Chuan–Mo Lee Ming–Lung Yu Sheng–Nan Lu Shun–Sheng Wu Li–Ying Liao Chi–Ling Chen Hsing–Tao Kuo You–Chen Chao Shui–Yi Tung Sien–Sing Yang Jia–Horng Kao Chen–Hua Liu Wei–Wen Su Chih–Lin Lin Yung–Ming Jeng Pei–Jer Chen Ding–Shinn | 2009 | Gastroenterology2009，，2： | 3 |
| 17 | Nitric Oxide Inducing Function and Intracellular Movement of Chicken Interleukin-18 in Cultured Cells显示文摘To evaluate the characteristics of chicken interleukin-18 (ChIL-18) in different forms in vitro,the ChIL-18 full-length gene (ChIL-18-F) and the ChIL-18 presumed mature protein gene (ChIL-18-M) werecloned and inserted into the eukaryotic expression vector pCI,to construct recombinant pCI-ChIL-18-Fand pCI-ChIL-18-M.The recombinant plasmids were then transferred into chicken splenic lymphocytes(CSLs).Western blot showed that ChIL-18-F,with a molecular weight of 23.0 kDa,was produced in CSLstransfected by pCI-ChlL-18-F;ChIL-18-M,with a molecular weight of 19.5 kDa,was produced in CSLstransfected by pCI-ChIL-18-M.The nitric oxide (NO) level in the transfected CSLs and the culture mediumat different time points was further examined under confocal microscopy using 4.5-diaminofluoresceinstaining.The results showed that both pCI-ChIL-18-F and pCI-ChIL-18-M groups showed significantincrease in intracellular and extracellular NO production compared with pCI transfected control cells.Theseresults suggest that both ChIL-18-F and ChIL-18-M could stimulate NO secretion in CSLs.To characterizethe intracellular distribution of ChIL-18,ChIL-18-F and ChIL-18-M were each fused to the enhanced greenfluorescent protein gene,and expressed in Vero cells.The results showed that the ChIL-18-F tended to themembranous region in Vero cells,while ChIL-18-M did not.This indicates that the N-terminal 27 amino acidpeptide helped ChIL-18 target to Veto cell membranes. | Jian XU Tong-Le DENG Long LI Zhen-Qiang YOU Wang-Jun WAN Lian YU | 2005 | Acta Biochimica et Biophysica Sinica2005，37，10： | 3 |
| 18 | OsPKpa_1 encodes a plastidic pyruvate kinase that affects starch biosynthesis in the rice endosperm显示文摘Pyruvate kinase(PK) is a key enzyme in glycolysis and carbon metabolism. Here, we isolated a rice(Oryza sativa) mutant, w59, with a white-core floury endosperm. Map-based cloning of w59 identified a mutation in OsPKpa_1, which encodes a plastidic isoform of PK(PKp). OsPKpa_1 localizes to the amyloplast stroma in the developing endosperm, and the mutation of OsPKpa_1 in w59 decreases the plastidic PK activity, resulting in dramatic changes to the lipid biosynthesis in seeds. The w59 grains were also characterized by a marked decrease in starch content. Consistent with a decrease in number and size of the w59 amyloplasts, large empty spaces were observed in the central region of the w59 endosperm, atthe early grain-filling stage. Moreover, a phylogenetic analysis revealed four potential rice isoforms of OsPKp. We validated the in vitro PK activity of these OsPKps through reconstituting active PKp complexes derived from inactive individual OsPKps, revealing the heteromeric structure of rice PKps, which was further confirmed using a protein–protein interaction analysis. These findings suggest a functional connection between lipid and starch synthesis in rice endosperm amyloplasts. | Yue Cai Wenwei Zhang Jie Jin Xiaoming Yang Xiaoman You Haigang Yan Liang Wang Jie Chen Jiahuan Xu Weiwei Chen Xingang Chen Jing Ma Xiaojie Tang Fei Kong Xiaopin Zhu Guoxiang Wang Ling Jiang William Terzaghi Chunming Wang Jianmin Wan | 2018 | Journal of Integrative Plant Biology2018，60，11： | 3 |
| 19 | Calibration procedures for the CHASE/HIS science data显示文摘The Hα line is an important optical line in solar observations containing the information from the photosphere to the chromosphere. To study the mechanisms of solar eruptions and the plasma dynamics in the lower atmosphere, the Chinese Hα Solar Explorer(CHASE) was launched into a Sun-synchronous orbit on October 14, 2021. The scientific payload of the CHASE satellite is the Hα Imaging Spectrograph(HIS). The CHASE/HIS acquires, for the first time, seeing-free Hα spectroscopic observations with high spectral and temporal resolutions. It consists of two observational modes. The raster scanning mode provides full-Sun or region-of-interest spectra at Hα(6559.7-6565.9 ?) and Fe I(6567.8-6570.6 ?) wavebands. The continuum imaging mode obtains full-Sun photospheric images at around 6689 ?. In this paper, we present detailed calibration procedures for the CHASE/HIS science data, including the dark-field and flat-field correction, slit image curvature correction, wavelength and intensity calibration, and coordinate transformation. The higher-level data products can be directly used for scientific research. | Ye Qiu ShiHao Rao Chuan Li Cheng Fang MingDe Ding Zhen Li YiWei Ni WenBo Wang Jie Hong Qi Hao Yu Dai PengFei Chen XiaoSheng Wan Zhi Xu Wei You Yuan Yuan HongJiang Tao XianSheng Li YuKun He Qiang Liu | 2022 | Science China(Physics,Mechanics & Astronomy)2022，65，8： | 3 |
| 20 | Synthesis and antitumor activity of novel 10-amino acids ester homocamptothecin analogues显示文摘九外消旋的 homocamptothecin 衍生物被综合,在 vitro antitumor,活动被标准 MTT 方法评估。结果比 iritecan 显示出复合有的更高的 antitumor 活动的那一些。 | Liang You Wan Nian Zhang Zhen Yuan Miao Wei Guo Xiao Ying Che Wen Ya Wang Chun Quan Sheng Jiang Zhong Yao Ting Zhou | 2008 | Chinese Chemical Letters2008，19，7： | 3 |
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