|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Macrophage polarization is involved in liver fibrosis induced byβ_(1)-adrenoceptor autoantibody显示文摘Accumulating evidence suggests that liver injury can be induced by the over-expression ofβ_(1)-adrenergic receptors(β_(1)-ARs).High titers of autoantibodies specific toβ_(1)-adrenergic receptors(β_(1)-AA)are detected in the sera of heart failure patients,potentially playing agonist-like roles.However,the role ofβ_(1)-AA in liver function has not been characterized.In this study,we collect the sera of primary biliary cholangitis(PBC)patients,a condition which easily develops into liver fibrosis,and analyze the relationship between PBC andβ_(1)-AA.A passive immunization model is established to assess the effect ofβ_(1)-AA on the liver.Subsequently,the effect ofβ_(1)-AA on macrophages is investigated in vitro.Results show that PBC patients have a high titer and ratio ofβ_(1)-AA,compared to controls.Liver injury and fibrosis are induced byβ_(1)-AA.In vitro experiments with ROS probe demonstrate thatβ_(1)-AA induces macrophages to produce ROS and secrete TNFα.These effects can be partially reversed by metoprolol,a blocker forβ_(1)-AR.Results from the transwell and phagocytosis assays show thatβ_(1)-AA promotes macrophage migration and phagocytosis.FCM tests suggest thatβ_(1)-AA induces the alteration of M1 rather than M2 markers in macrophages.Finally,the Annexin V/PI assay indicates that macrophage culture supernatants stimulated byβ_(1)-AA cause hepatocyte apoptosis.Overall,these results suggest thatβ_(1)-AA is involved in PBC.Theβ_(1)-AA-induced activation,phagocytosis and phenotypic modification of macrophages may play an important role in the development of hepatic fibrosis and injury. | Ye Wu Xiongxiong Fan Haicun Yu Jingyi Liu Yanru Duan Suli Zhang Li Yan Yunhui Du Huirong Liu | 2022 | Acta Biochimica et Biophysica Sinica2022，54，8： | 1 |
| 2 | Deletion of large-conductance calcium-activated potassium channels promotes vascular remodelling through the CTRP7-mediated PI3K/Akt signaling pathway显示文摘The large-conductance calcium-activated potassium(BK)channel is a critical regulator and potential therapeutic target of vascular tone and architecture,and abnormal expression or dysfunction of this channel is linked to many vascular diseases.Vascular remodelling is the early pathological basis of severe vascular diseases.Delaying the progression of vascular remodelling can reduce cardiovascular events,but the pathogenesis remains unclear.To clarify the role of BK channels in vascular remodelling,we use rats with BK channelαsubunit knockout(BKα‒/‒).The results show that BKα‒/‒rats have smaller inner and outer diameters,thickened aortic walls,increased fibrosis,and disordered elastic fibers of the aortas compared with WT rats.When the expression and function of BKαare inhibited in human umbilical arterial smooth muscle cells(HUASMCs),the expressions of matrix metalloproteinase 2(MMP2),MMP9,and interleukin-6 are enhanced,while the expressions of smooth muscle cell contractile phenotype proteins are reduced.RNA sequencing,bioinformatics analysis and qPCR verification show that C1q/tumor necrosis factor-related protein 7(CTRP7)is the downstream target gene.Furthermore,except for that of MMPs,a similar pattern of IL-6,smooth muscle cell contractile phenotype proteins expression trend is observed after CTRP7 knockdown.Moreover,knockdown of both BKαand CTRP7 in HUASMCs activates PI3K/Akt signaling.Additionally,CTRP7 is expressed in vascular smooth muscle cells(VSMCs),and BKαdeficiency activates the PI3K/Akt pathway by reducing CTRP7 level.Therefore,we first show that BK channel deficiency leads to vascular remodelling.The BK channel and CTRP7 may serve as potential targets for the treatment of cardiovascular diseases. | Jing Bi Yanru Duan Meili Wang Chunyu He Xiaoyue Li Xi Zhang Yan Tao Yunhui Du Huirong Liu | 2022 | Acta Biochimica et Biophysica Sinica2022，54，12： | 0 |
| 3 | Decavanadate-based Transition Metal Hybrids as Bifunctional Catalysts for Sulfide Oxidation and C-C Bond Construction显示文摘The development of bifunctional catalysts has drawn much attention in realizing efficient and feasible catalytic systems to meet the diverse dema nd of pote ntial industrial applications.Desig n of stable and powerful bifun ctional catalysts for various catalysis systems is highly desirable yet largely unmet.Here,three kinds of decavanadate-based transition metal hybrids(DTMH)(i.e.,Co-DTMH,Ni-DTMH and Ag-DTMH)have been successfully synthesized through a pH tuning strategy and further characterized.Specifically,the rare M05N six-coordinated transition metal coordination modes have been detected in Co-DTMH and Ni-DTMH,while Ag atoms in Ag-DTMH exhibited three-and five-coordinated geometries with the tuning of specially selected imidazole ligands.Thus-obtained clusters can serve as powerful bifunctional catalysts for both sulfide oxidation and C-C bond construction.Remarkably,Ag-DTMH dem on st rated excellent heteroge ne ous bifunctional catalytic properties in the selective oxidati on of sulfides and construction of C-C bond(yields up to 99%),which enable successful recycling for three cycles with remained catalytic activities and structure stability.The newly designed decavanadate-based transition metal hybrids with bifunctional property hold high promise in the practical applications like continuous catalysis or flow bed reactions. | Xianqiang Huang Xiaoyu Gu Yuquan Qi Yanru Zhang Guodong Shen Bingchuan Yang Wenzeng Duan Shuwen Gong Zechun Xue Yifa Chen | 2021 | Chinese Journal of Chemistry2021，39，9： | 0 |
| 4 | Tie2-expressing monocytes/macrophages promote cerebral revascularization in peri-infarct lesions upon ischemic insult显示文摘Dear Editor,Strokes cause 5.8 million deaths each year.Among these victims,~30% are from China1.Acute ischemic stroke(AIS)is the most prevalent subtype of strokes.Although drugs can alleviate the symptoms,the recoveries of functional vessels within ischemic areas are the critical factor determining the prognosis of patients suffering from AIS2.Nevertheless,the mechanisms involved in cerebral revascularization remain largely unknown. | Yuqiao Sheng Xixi Duan Yanru Liu Feng Li Shengli Ma Xiaoping Shang Xiao Wang Yangyang Liu Rui Xue Zhihai Qin | 2021 | Signal Transduction and Targeted Therapy2021，6，9： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员