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| 1 | Homogeneous label-free fluorescent assay of small molecule-protein interactions using protein binding-inhibited transcription nanomachine显示文摘Quantitative analysis of interactions between small molecules and proteins is a central challenge in chemical genetics, molecular diagnostics and drug developments. Here, we developed a RNA transcription nanomachine by assembling T7 RNA polymerase on a small molecule-labeled DNA heteroduplex. The nanomachine, of which the RNA transcription activity can be quantitatively inhibited by protein binding, showed a great potential for small molecule-protein interaction assay. This finding enabled us to develop a novel homogeneous label-free strategy for assays of interactions between small molecules and their protein receptors. Three small molecule compounds and their protein receptors have been used to demonstrate the developed strategy. The results revealed that the protein-small molecule interaction assay strategy shows dynamic responses in the concentration range from 0.5 to 64 nM with a detection limit of 0.2 nM. Due to its label-free, homogeneous, and fluorescence-based detection format, besides its desirable sensitivity this technique could be greatly robust, cost-efficient and readily automated, implying that the developed small molecule-protein interaction assay strategy might create a new methodology for developing intrinsically robust, sensitive and selective platforms for homogeneous protein detection. | ZHOU DianMing, WU YiDan, LIU Pei, BAI HaoTian, TANG LiJuan, YU RuQin & JIANG JianHui State Key Laboratory of Chemo/Bio-Sensing and Chemometrics College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, China | 2011 | Science China Chemistry2011，54，8： | 4 |
| 2 | Genome-wide dissection of segregation distortion using multiple inter-subspecific crosses in rice显示文摘Mendelian inheritance can ensure equal segregation of alleles from parents to offspring, which provides fundamental basis for genetics and molecular biology. Segregation distortion(SD) leads to preferential transmission of certain alleles from generation to generation. Such violation of Mendelian genetic principle is often accompanied by reproductive isolation and eventually speciation. Although SD is observed in a wide range of species from plants to animals, genome-wide dissection of such biased transmission of gametes is rare. Using nine inter-subspecific rice crosses, a genome-wide screen for SD loci is performed, which reveals 61 single-locus quantitative trait loci and 194 digenic interactions showing distorted transmission ratio, among which 24 new SD loci are identified. Biased transmission of alleles is observed in all nine crosses, suggesting that SD exists extensively in rice populations. 72.13% distorted regions are repeatedly detected in multiple populations, and the most prevalent SD hotspot that observed in eight populations is mapped to chromosome 3. Xian alleles are transmitted at higher frequencies than geng alleles in inter-subspecific crosses, which change the genetic composition of the rice populations. Epistatic interaction contributes significantly to the deviation of Mendelian segregation at the whole-genome level in rice, which is distinct from that in animals. These results provide an extensive archive for investigating the genetic basis of SD in rice, which have significant implications in understanding the reproductive isolation and formation of inter-subspecific barriers during the evolution. | Guangwei Li Jiye Jin Yan Zhou Xufeng Bai Donghai Mao Cong Tan Gongwei Wang Yidan Ouyang | 2019 | Science China(Life Sciences)2019，62，4： | 4 |
| 3 | Degradable Hybrid CuS Nanoparticles for Imaging-Guided Synergistic Cancer Therapy via Low-Power NIR-II Light Excitation显示文摘Near-infrared(NIR)-II light-excitable photonic agents capable of generating tumor hyperthermia and cytotoxic free radicals are promising for synergistic phototherapy of tumors.However,the lack of NIR-II excitable agents makes it challenging to achieve combinational tumor phototherapy.Here,the authors have reported on a tumor-targeting and degradable hybrid copper sulfide(CuS)nanoparticle(AIBA@CuS-FA)via loading a hydrophilic Azo initiator(AIBA)into an amphiphilic lipid-encapsulating CuS nanoparticle.AIBA@CuS-FA shows high photothermal conversion efficiency(PCE≈47.5%)at 1064 nm,enabling heat production to trigger tumor hyperthermia and thermal decomposition of AIBA into cytotoxic free alkyl radicals upon irradiation with a 1064-nm laser under low-power density(0.5 W/cm2).Moreover,alkyl radicals can drive degradation of AIBA@CuS-FA and embedded CuS nanodisks,releasing Cu^(2+)ions that can catalyze a Fenton-like reaction for hydroxyl radical(•OH)production to promote tumor therapy.Findings demonstrate promise for combinational photothermal therapy(PTT),oxygen-independent alkyl radical therapy,and chemodynamic therapy(CDT)of tumors. | Yidan Sun Hua Shi Xiaoyang Cheng Luyan Wu Yuqi Wang Zhengyang Zhou Jian He Hong-Yuan Chen Deju Ye | 2021 | CCS Chemistry2021，3，5： | 2 |
| 4 | NUPR1 promotes the proliferation and metastasis of oral squamous cell carcinoma cells by activating TFE3-dependent autophagy显示文摘Oral squamous cell carcinoma(OSCC)is the most common type of oral malignancy,and metastasis accounts for the poor prognosis of OSCC.Autophagy is considered to facilitate OSCC development by mitigating various cellular stresses;nevertheless,the mechanisms of autophagy in OSCC cell proliferation and metastasis remain unknown.In our study,high-sensitivity label-free quantitative proteomics analysis revealed nuclear protein 1(NUPR1)as the most significantly upregulated protein in formalin-fixed paraffin-embedded tumour samples derived from OSCC patients with or without lymphatic metastasis. | Tengfei Fan Xiaoning Wang Sheng Zhang Ping Deng Yi Jiang Yidan Liang Sheng Jie Qing Wang Chuwen Li Guocai Tian Zhen Zhang Zhenhu Ren Bo Li Yanrong Chen Zhijing He Yan Luo Mingliang Chen Hanjiang Wu Zhengping Yu Huifeng Pi Zhou Zhou Zhiyuan Zhang | 2022 | Signal Transduction and Targeted Therapy2022，7，5： | 2 |
| 5 | Synthesis and properties of UV -curable hyperbranched polyurethane acrylate oligomers containing photoinitiator 显示文摘 | WENSONG HAN BAOPING LIN YIDAN ZHOU | 2012 | Polymer Bulletin2012，68，： | 1 |
| 6 | Synthesis and properties of UV-curable hyperbranched polyurethane acrylate oligomers containing photoinitiator显示文摘 | Wensong Han Baoping Lin Yidan Zhou Jiangang Song | 2012 | Polymer Bulletin2012，，3： | 1 |
| 7 | A high-strength self-healing nano-silica hydrogel with anisotropic differential conductivity显示文摘Soft nano electronic materials based on conductive hydrogels have attracted considerable attention due to their exceptional properties.Particle deposition and poor interface compatibility often diminish the mechanical strength and electron transport capabilities of the conductive hydrogel.Mechanical damage can severely impact the performance of the conductive hydrogel and can even damage electronic devices based on the conductive hydrogel.In the current study,a transparent nano-silica hydrogel is prepared by employing an extremely easy-to-operate method.This approach can preclude the deposition of particles via strong mechanical force.In addition,controlling the concentration of the reaction interface makes the hydrogel grow along the mechanical force in the direction with a special directional hole structure formed.The hydrogel is transparent,showing excellent self-healing properties—it can self-heal within 15 seconds.Remarkably,the hydrogel after self-healing maintains its performance.Moreover,it has excellent mechanical properties and can be stretched in length.Up to 1,200%of the original length,the tensile strength of the gel spline can reach 7 MPa.The viscosity of the hydrogel can reach 1.67×10^(8)(MPs).In addition,a large amount of Na+in this hydrogel endow it a conductivity of 389 ps/cm.The conductivity of this hydrogel is adjustable result from the special pore structure.Lastly,the difference between the horizontal and vertical conductivity of the same sample can reach 3-4 times,thus this hydrogel can be used in the field of nano conductive materials. | Xingyu Huang Xiaofan Zhou Hao Zhou Yidan Zhong Hui Luo Fan Zhang | 2021 | Nano Research2021，14，8： | 1 |
| 8 | Ti2AlC triggered in-situ ultrafine TiC/Inconel 718 composites:Microstructure and enhanced properties显示文摘In situ ultrafine TiC dispersion reinforced Inconel 718 alloy with enhanced mechanical properties was fabricated by the technique of reactive hot-press sintering Ti2AlC and In718 powders.The effect of Ti2AlC precursor additions(5 vol.%,10 vol.%,15 vol.%)on microstructure and mechanical properties of TiC/In718 composites were investigated.A relationship of microstructural characteristics,room and elevated temperature mechanical performance,and underlying strengthening mechanisms were analyzed.The results show that initial Ti2AlC precursor transformed completely into ultrafine TiC particulate(~230 nm)and distributed uniformly in the matrix after sintering 5 and 10 vol.%Ti2AlC/In718.However,TiC particulates tended to aggregate to stripes with the addition of Ti2AlC up to 15 vol.%,which,in adverse,weaken the properties of In718.The 5 vol.%Ti2AlC/In718 sample showed a higher tensile strength of 1404±13 MPa with a noticeable elongation of 9.8%at room temperature compared to the pure In718(ultimate tensile strength(UTS)=1310 MPa,elongation=21.5%).At 600℃,700℃,800℃and 900℃,tensile strength of the as-sintered 5 vol.%Ti2AlC/In718 composite was determined to be 1333±13 MPa,1010±10 MPa,685±25 MPa and 276±3 MPa,increased by 9.2%,14.6%,14.2%and 55%,respectively,compared with that of monolithic In718 alloy.The excellent tensile properties of TiC/In718 composite can be ascribed to the combined mechanisms in term of increased dislocation density,dispersive Orowan and load transfer mechanisms. | Wenqiang Hu Zhenying Huang Qun Yu Yuanbo Wang Yidan Jiao Cong Lei Leping Cai Hongxiang Zhai Yang Zhou | 2020 | Journal of Materials Science & Technology2020，47，16： | 1 |
| 9 | Synthesis and properties of UV-curable hyperbranched polyurethane acrylate oligomers containing photoinitiator 显示文摘 | Han Wensong Lin Baoping Zhou Yidan | 2012 | Polym Bull2012，68，3： | 1 |
| 10 | Synthesis and properties of UV-curable hyperbranched polyurethane acrylate oligomers containing photoinitiator显示文摘 | Wensong Han Baoping Lin Yidan Zhou Jiangang Song | 2012 | Polymer Bulletin2012，，3： | 1 |
| 11 | An ultrapotent pan-β-coronavirus lineage B(β-CoV-B)neutralizing antibody locks the receptor-binding domain in closed conformation by targeting its conserved epitope显示文摘New threats posed by the emerging circulating variants of SARS-CoV-2 highlight the need to find conserved neutralizing epitopes for therapeutic antibodies and efficient vaccine design.Here,we identified a receptorbinding domain(RBD)-binding antibody,XG014,which potently neutralizesβ-coronavirus lineage B(β-CoV-B),including SARS-CoV-2,its circulating variants,SARSCoV and bat SARSr-CoV WIV1.Interestingly,antibody family members competing with XG014 binding show reduced levels of cross-reactivity and induce antibodydependent SARS-CoV-2 spike(S)protein-mediated cellcell fusion,suggesting a unique mode of recognition by XG014.Structural analyses reveal that XG014 recognizes a conserved epitope outside the ACE2 binding site and completely locks RBD in the non-functional“down”conformation,while its family member XG005 directly competes with ACE2 binding and position the RBD“up”.Single administration of XG014 is effective in protection against and therapy of SARS-CoV-2 infection in vivo.Our findings suggest the potential to develop XG014 as pan-β-CoV-B therapeutics and the importance of the XG014 conserved antigenic epitope for designing broadly protective vaccines againstβ-CoV-B and newly emerging SARS-CoV-2 variants of concern. | Zezhong Liu Wei Xu Zhenguo Chen Wangjun Fu Wuqiang Zhan Yidan Gao Jie Zhou Yunjiao Zhou Jianbo Wu Qian Wang Xiang Zhang Aihua Hao Wei Wu Qianqian Zhang Yaming Li Kaiyue Fan Ruihong Chen Qiaochu Jiang Christian TMayer Till Schoofs Youhua Xie Shibo Jiang Yumei Wen Zhenghong Yuan Kang Wang Lu Lu Lei Sun Qiao Wang | 2022 | Protein & Cell2022，13，9： | 1 |
| 12 | minimal genome design to maximally guarantee fertile inter-subspecific hybrid rice显示文摘Hybrid rice has made considerable contributions to achieve the ambitious goal of food security for the world's population.Hybrid rice from indica/xian and japonica/geng subspecies shows much higher heter-osis and is thereby an important innovation in promoting rice production in the next decade.However,such inter-subspecific hybrid rice has long suffered from serious hybrid sterility,which is a major challenge that needs to be addressed.In this study,we performed a genome design strategy to produce fertile inter-subspecific hybrid by creation of wide compatibility varieties that are able to overcome hybrid sterility.Based on combined genetic analyses in two indica-japonica crosses,we determined that four hybrid ste-rility loci,S5,f5,pf12 and Sc,are the major QTLs controlling inter-subspecific hybrid sterility and thus the minimal targets that can be manipulated for breeding sub-specific hybrid rice.We then cloned the pf12 lo-cus,one of the most effective loci for hybrid male sterility,by map-based cloning,and showed that artificial disruption of pf12A gene at this locus could successfully rescue hybrid fertility.We further dissected the genetic basis of wide compatibility using three pairwise crosses from a wide-compatibility variety Dular and representative indica and japonica varieties.On this basis,we constructed and assembled different combinations of naturally compatible alleles of four loci,S5,Sc,pf12,and f5,and found that the improved lines could fully recover pollen and embryo sac fertility in test-crossed F,s,thereby completely fulfilling the demands of inter-subspecific hybrid spikelet fertility in agricultural production.This breeding scheme would facilitate redesign of future inter-subspecific hybrid rice with a higher yield potential. | Penghui Zhou Zhengji Wang Xingchen Zhu Yao Tang Liang Ye Huihui Yu Yating Li Ningke Zhang Ting Liu Tian Wang Yuying Wu Dengyun Cao Yuan Chen Xu Li Qinglu Zhang Jinghua Xiao Sibin Yu Qifa Zhang Jiaming Mi Yidan Ouyang | 2023 | Molecular Plant2023，16，4： | 0 |
| 13 | Photoelectrocatalytic hydrogen evolution and synchronous degradation of organic pollutants by pg-C_(3)N_(4)/β-FeOOH S-scheme heterojunction显示文摘Crafting photoelectrocatalytic materials with robust oxidation-reduction properties for simultaneous hydrogen evolution and pollutant degradation poses a formidable challenge.In this study,a pg-C_(3)N_(4)/β-FeOOH S-scheme heterostructure with a special energy band structure was developed by anchoring porous pg-C_(3)N_(4)on needle shapedβ-FeOOH.Functioning as a hole extraction layer,needle-leaf-likeβ-FeOOH can facilitate efficient hole migration and enhance charge transport.Remarkably,the optimized 0.2-pg-C_(3)N_(4)/β-FeOOH could degrade 78%of ofloxacin(OFLO)in 90 min.The organic pollutants could absorb a large number of holes,which prompted a greater proportion of photogenerated electrons to actively participate in the hydrogen evolution reaction at the cathode.Consequently,the hydrogen production of 0.2-pg-C_(3)N_(4)/β-FeOOH reached 1452.88μmol cm^(-2)h^(-1),exhibiting a notable increase of 61.81-165.12μmol cm^(-2)h^(-1)compared with that in the absence of pollutants.Experimental and theoretical calculation results underscore that this investigation is grounded in a distinctive electron and hole dual channel transfer mechanism.These findings offer novel insights for the future development of S-scheme heterojunction photoelectrocatalytic materials capable of concurrently degrading pollutants and promoting hydrogen evolution. | LI XiBao HAN Tao ZHOU YingTang XIE Yu LUO YiDan HUANG JunTong CHEN Zhi DENG Fang | 2024 | Science China(Technological Sciences)2024，67，4： | 0 |
| 14 | Waggle needling Yanglingquan(GB34) enhances cerebral blood flow and ameliorates condition of spastic paresis in rats with middle cerebral artery occlusion induced by surgical nylon suture显示文摘OBJECTIVE:To evaluate the therapeutic efficacy and the influence on cerebral blood supply of waggle needling Yanglingquan(GB34) on spastic paresis(SP) rats after middle cerebral artery occlusion(MCAO) induced and investigate its mechanism of relieving neurobehavior deficiency.METHODS:SP rat model was produced by permanent MCAO.Rats were divided into five groups:blank control group(Control),sham operation group(Sham),model group(Model),waggle needling group(WN) and perpendicular needling group(PN).SP rats were treated with acupuncture from day 3 after MCAO,once a day for 6 d.The modified neurological severity score(m NSS) and modified Ashworth scale(MAS) were conducted on days 0,1,3,5,7 and 9.Cerebral blood flow(CBF) in ischemic cortex was measured by laser speckle imaging 5 min pre ischemia,5 min post ischemia,and after intervention on day 9.All rats were sacrificed at day 9 and the protein and m RNA expressions of γ2 subunit of the γ-aminobutyric acid receptor A(GABAAγ2) and K+-Cl-cotransporter 2(KCC2) in the ischemic cortex and lumber enlargement was measured by Western blotting and real-time quantitative polymerase chain reaction.RESULTS:Both Control and Sham groups showed no changes in m NSS and MAS scores and in the regional CBF.Compared with Model group,both WN and PN treatments significantly ameliorated neurological deficit(P < 0.01),decreased muscle tone(P < 0.05),and enhanced CBF(P < 0.001) in SP rats;moreover,WN showed superior effects than PN(P < 0.001).In line with the improvement in neurobehavior,acupuncture interventions up-regulated the expressions of GABAAγ2 and KCC2 in the ischemic cortex as well as lumber enlargement(P < 0.01) in SP rats,and those changes were more obvious in WN(P < 0.05).CONCLUSIONS:Acupuncture at Yanglingquan(GB34) enhanced cerebral blood flow and ameliorated SP in permanent MCAO rats,while waggle needling was superior to regular perpendicular needling.Waggle needling Yanglingquan(GB34) would be a potential complementary therapy for SP. | ZHANG Zhou MU Jiedan MA Liangxiao YU Wenyan SUN Tianyi QIAN Xu TIAN Yuan ZHANG Yidan WANG Junxiang | 2023 | Journal of Traditional Chinese Medicine2023，43，3： | 0 |
| 15 | Correction to:NUPR1 promotes the proliferation and metastasis of oral squamous cell carcinoma cells by activating TFE3-dependent autophagy显示文摘In the process of checking the raw data1,the authors noticed several inadvertent mistakes occurring in Fig.5a,b,c,d,f that need to be corrected after online publication of the article.During the preparation of Fig.5,the representative image showing TFE3 overexpression antagonized the NUPR1 KD-induced inhibition of OSCC cell proliferation and metastasis,were pasted and placed by mistake.The correct results should be as shown below.The authors apologize for these oversights and declare that these corrections do not affect the description,interpretation,or conclusions detailed in the original manuscript. | Tengfei Fan Xiaoning Wang Sheng Zhang Ping Deng Yi Jiang Yidan Liang Sheng Jie Qing Wang Chuwen Li Guocai Tian Zhen Zhang Zhenhu Ren Bo Li Yanrong Chen Zhijing He Yan Luo Mingliang Chen Hanjiang Wu Zhengping Yu Huifeng Pi Zhou Zhou Zhiyuan Zhang | 2022 | Signal Transduction and Targeted Therapy2022，7，10： | 0 |
| 16 | Hybrid Optical-Electrical Perovskite Can Be a Ferroelastic Semiconductor显示文摘Organic–inorganic hybrid perovskites(OIHPs)have been a hot research topic due to their advanced structural and functional features that cover almost all the research fields of intelligent materials including ferroelectric,photovoltaic,fluorescent,and dielectric.However,the development of the construction of an OIHP ferroelastic semiconductor with optical-electrical response has been a huge challenge and infrequently reported.In this work,a rare and interesting hybrid perovskite ferroelastic semiconductor,[BFDA]PbBr_(3)(BFDA=benzyl-(2-fluoro-ethyl)-dimethyl-ammonium),was synthesized,which benefits from the structural advantage of a long tail BFDA to be balanced by the suitable inorganic framework.[BFDA]PbBr_(3) shows a high-temperature ferroelastic phase transition at 365 K and a direct band gap of 3.33 eV.In addition,it can emit charming orange-pink light under a 365 nm UV lamp.To combine this with the ferroelastic,optical,and dielectric properties,[BFDA]PbBr_(3) can be identified as a very rarely reported ferroelastic semiconductor.The above-mentioned synthesis strategy is also helpful for the enrichment and development of the hybrid perovskite family. | Changyuan Su Mengmeng Lun Yidan Chen Yichen Zhou Zhixu Zhang Ming Chen Peizhi Huang Dawei Fu Yi Zhang | 2022 | CCS Chemistry2022，4，6： | 0 |
| 17 | Selection and structural bases of potent broadly neutralizing antibodies from 3-dose vaccinees that are highly effective against diverse SARS-CoV-2 variants,including Omicron sublineages显示文摘Dear Editor,The severe acute respiratory syndrome coronavirus 2(SARS-CoV-2),belonging to the lineage B of the genus Betacoronavirus in the Coronaviridae family,is the causative agent of the C0VID19 pandemic,1 which has lasted for over two years,resulting in unprecedented public health and socioeconomic crisis.Several SARS-CoV-2 variants of interest(VOIs)as well as variants of concern(VOCs),including the Alpha(B.1.1.7),Beta(B.1.351),Gamma(P.l),Delta(B.1.617.2)and the most recently identified Omicron sublineages(BA.1,BA.1.1,BA.2 and BA.3),have emerged at different time points over the course of the pandemic,leading to the resurge nee of outbreaks across the world.Among these variants. | Lei Wang Wangjun Fu Linlin Bao Zijing Jia Yuxia Zhang Yunjiao Zhou Wei Wu Jianbo wu Qianqian Zhang Yidan Gao Kang Wang Qiao Wang Chuan Qin Xiangxi Wang | 2022 | Cell Research2022，32，7： | 0 |
| 18 | Theoretical investigation of loading Ni clusters on the α-Ga_2O_3 surfaces for photocatalytic hydrogen evolution显示文摘In the semiconductor-based photocatalysts for overall water splitting, loading proper cocatalysts play a crucial role in enhancing the photocatalytic activity. In this work, we have chosen Ni_n/α-Ga_2O_3 as a model and provided detailed density functional theory calculations to investigate the function of cocatalysts in hydrogen evolution reaction(HER). We have studied the formation and stability of Ni_n(n = 1–4) cluster on two stable surfaces of α-Ga_2O_3(001) and(012). In a Ni_n/α-Ga_2O_3 system, as the Ni 3d states well overlap with O and Ga states, the excited electrons transferred from Ga to Ni may participate in HER. We theoretically predict that introduction of Nincluster on(012) surface can elevate the Fermi level toward the conduction band, which is favorable for the occurrence of HER. Electrochemical computations are used to explore the mechanism of HER. It is found that, in most of Ni_n/α-Ga_2O_3 systems, the active sites of HER are on Ni_n clusters. Loading Ni_n clusters not only importantly reduces the Gibbs free energy of HER but also improves the reaction activity of surface O and Ga sites in HER. Our calculations reasonably explain the experimental observation on significant enhancement of activity for generating hydrogen after loading nickel oxide cocatalysts. | Jiaxin Zhang Yidan Wang Hao Dong Xin Zhou | 2019 | Journal of Energy Chemistry2019，28，3： | 0 |
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