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| 1 | In vitro and in vivo activity of D-serine in combination with β-lactam antibiotics against methicillin-resistant Staphylococcus aureus显示文摘As D-amino acids play important roles in the physiological metabolism of bacteria,combination of D-amino acids with antibiotics may provide synergistic antibacterial activity. The aim of the study was to evaluate in vitro and in vivo activity of D-serine alone and in combination withβ-lactams against methicillin-resistant Staphylococcus aureus(MRSA) strains, and to explore the possible sensitization mechanisms. The activity of D-serine, β-lactams alone and in combinations was evaluated both in vitro by standard MICs, time–kill curves and checkerboard assays, and in vivo by murine systemic infection model as well as neutropenic thigh infection model. An in vitro synergistic effect was demonstrated with the combination of D-serine and β-lactams against MRSA standard and clinical strains.Importantly, the combinations enhanced the therapeutic efficacy in the animal models as compared toβ-lactam alone groups. Initial mechanism study suggested possible revision of D-alanine-D-alanine residue to D-alanine-D-serine in peptidoglycan by adding of D-alanine in the medium, which may cause decreased affinity to PBPs during transpeptidation. In conclusion, D-serine had synergistic activity in combination with β-lactams against MRSA strains both in vitro and in vivo. Considering the relatively good safety of D-serine alone or in combination with β-lactams, D-serine is worth following up as new anti-MRSA infection strategies. | Qing Wang Yuemeng Lv Jing Pang Xue Li Xi Lu Xiukun Wang Xinxin Hu Tongying Nie Xinyi Yang Yan Q.Xiong Jiandong Jiang Congran Li Xuefu You | 2019 | Acta Pharmaceutica Sinica B2019，9，3： | 6 |
| 2 | Efficient generation of the mouse model with a defined point mutation through haploid cell-mediated gene editing显示文摘Generation of mouse models carrying a defined point mutation,especially disease-related point mutations,is of considerable interest for research in biology and medicine.The standard method based on embryonic stem cell(ESC)-mediated homologous recombination(HR)is time-and labor-consuming.CRISPR-Cas9 system has been rapidly developed recently and adopted for efficient gene-editing in different species(Mei et al.,2016).Remarkably。 | Leixin Wei Xiukun Wang Suming Yang Wen Yuan Jinsong Li | 2017 | Journal of Genetics and Genomics2017，44，9： | 6 |
| 3 | Gas sorption and non-Darcy flow in shale reservoirs显示文摘Gas sorption and non-Darcy flow are two important issues for shale gas reservoirs. The sorption consists of dissolution and adsorption. Dissolved gas and adsorbed gas are different. The former is dissolved in the shale matrix, while the latter is concentrated near the solid walls of pores. In this paper, the Langmuir equation is used to describe adsorption and Henry’s law is used to describe dissolution. The K coefficient in Henry’s law of 0.052 mmol/(MPa g TOC) is obtained by matching experimental data. The amount of dissolved gas increases linearly when pressure increases. Using only the Langmuir equation without considering dissolution can lead to a significant underestimation of the amount of sorbed gas in shales. For non-Darcy gas flow, the apparent permeability model for free gas is established by combining slip flow and Knudsen flow. For adsorbed gas, the surface diffusion effect is also considered in this model. The surface diffu- sion coefficient is suggested to be of the same scale as the gas self-diffusion coefficient, and the corresponding effective permeability is derived. When 1/ increases,k_(app)/ k_D increases, but the relationship is not linear as the Klinkenberg effect suggests. The effect of adsorption on the gas flow is significant in nanopores (r≤2 nm). Adsorption increases apparent permeability in shales at low pressures and decreases it at high pressures. | Xiukun Wang James Sheng | 2017 | Petroleum Science2017，14，4： | 3 |
| 4 | Genetic basis of high level aminoglycoside resistance in Acinetobacter baumannii from Beijing,China显示文摘The objective of this study was to investigate the genetic basis of high level aminoglycoside resistance in Acinetobacter baumannii clinical isolates from Beijing,China.173 A.baumannii clinical isolates from hospitals in Beijing from 2006 to 2009 were first subjected to high level aminoglycoside resistance(HLAR,MIC to gentamicin and amikacin>512 mg/mL)phenotype selection by broth microdilution method.The strains were then subjected to genetic basis analysis by PCR detection of the aminoglycoside modifying enzyme genes(aac(3)-Ⅰ,aac(3)-Ⅱc,aac(60)-Ⅰb,aac(60)-Ⅱ,aph(4)-Ⅰa,aph(30)-Ⅰ,aph(30)-Ⅱb,aph(30)-Ⅲa,aph(30)-Ⅵa,aph(2″)-Ⅰb,aph(2″)-Ⅰc,aph(2″)-Ⅰd,ant(2″)-Ⅰa,ant(3″)-Ⅰand ant(40)-Ⅰa)and the 16S rRNA methylase genes(armA,rmtB and rmtC).Correlation analysis between the presence of aminoglycoside resistance gene and HLAR phenotype were performed by SPSS.Totally 102(58.96%)HLAR isolates were selected.The HLAR rates for year 2006,2007,2008 and 2009 were 52.63%,65.22%,51.11%and 70.83%,respectively.Five modifying enzyme genes(aac(3)-Ⅰ,detection rate of 65.69%;aac(60)-Ⅰb,detection rate of 45.10%;aph(30)-Ⅰ,detection rate of 47.06%;aph(30)-Ⅱb,detection rate of 0.98%;ant(3″)-Ⅰ,detection rate of 95.10%)and one methylase gene(armA,detection rate of 98.04%)were detected in the 102 A.baumannii with aac(3)-Ⅰ+aac(60)-Ⅰ+þant(3″)-Ⅰ+armA(detection rate of 25.49%),aac(3)-Ⅰ+aph(30)-Ⅰ+ant(3″)-Ⅰ+armA(detection rate of 21.57%)and ant(3″)-Ⅰ+armA(detection rate of 12.75%)being the most prevalent gene profiles.The values of chi-square tests showed correlation of armA,ant(3″)-Ⅰ,aac(3)-Ⅰ,aph(30)-Ⅰand aac(60)-Ⅰb with HLAR.armA had significant correlation(contingency coefficient 0.685)and good contingency with HLAR(kappa 0.940).The high rates of HLAR may cause a serious problem for combination therapy of aminoglycoside with β-lactams against A.baumannii infections.As armA was reported to be able to cause high level aminoglycoside resistance to most of the clinical important aminoglycosides(gentamicin,amikacin,tobramycin,etc),the function of aminoglycoside modifying enzyme gene(s)in A.baumannii carrying armA deserves further investigation. | Lu Nie Yuemeng Lv Min Yuan Xinxin Hu Tongying Nie Xinyi Yang Guoqing Li Jing Pang Jingpu Zhang Congran Li Xiukun Wang Xuefu You | 2014 | Acta Pharmaceutica Sinica B2014，4，4： | 3 |
| 5 | NIR Chemical-Image Analysis Based on Adaptive Local Optimization PSO显示文摘Principal component analysis(PCA) combined with cluster analysis has become an effective approach for Near-infrared(NIR) chemical image analysis.Traditional cluster algorithms are sensitive to initial starting conditions and can be trapped into local optimal solutions. To overcome the drawbacks, we develop a new algorithm in this paper which improves Particle swarm optimization with Adaptive local optimization(ALO-PSO).Simulation experiments performed on NIR image of tablet verify the feasibility and effectiveness of the proposed algorithm. Experimental results of the clustering performances indicate that ALO-PSO algorithm offers an alternative approach for solving data clustering problems in NIR chemical image analysis. | YANG Xiukun ZHONG Mingliang JING Xiaojun WANG Jianli CHEN Xuqi | 2014 | Chinese Journal of Electronics2014，23，1： | 2 |
| 6 | New method for discretization of continuous attributes in rough set theory显示文摘A new method for discretization of continuous attributes is put forward to overcome the limitation of the traditional rough sets,which cannot deal with continuous attributes.The method is based on an improved algorithm to produce candidate cut points and an algorithm of reduction based on variable precision rough information entropy.With the guarantee of consistency of decision system,the method can reduce the number of cut points and improve efficiency of reduction.Adopting variable precision rough information entropy as measure criterion,it has a good tolerance to noise.Experiments show that the algorithm yields satisfying reduction results. | Rong Cong Xiukun Wang Kai Li Nanhai Yang | 2010 | Journal of Systems Engineering and Electronics2010，21，2： | 2 |
| 7 | Multi-Component and Multi-Point Trace Gas Sensing in Wavelength Modulation Spectroscopy Based on Wavelength Stabilization显示文摘Multi-component and multi-point trace gas sensing in the wavelength modulation spectroscopy is demonstrated based on the frequency-division multiplexing and time-division multiplexing technology.A reference photodetector is connected in series with a reference gas cell with the constant concentration to measure the second-harmonics peak of the components for wavelength stabilization in real time.The central wavelengths of the distributed feedback lasers are locked to the target gas absorption centers by the reference second-harmonics signal using a digital proportional-integral-derivative controller.The distributed feedback lasers with different wavelengths and modulation frequencies are injected into the gas cell to achieve multi-components gas measurement by the frequency-division multiplexing technology.In addition,multi-point trace gas sensing is achieved by the time-division multiplexing technology using a photoswitch and a relay unit.We use this scheme to detect methane(CH4)at 1650.9 nm and water vapor(H2O)at 1368.597 nm as a proof of principle with the gas cell path length of 10 cm.The minimum detection limits achieved for H2O and CH4 are 1.13 ppm and 11.85 ppm respectively,with three-point gas cell measurement;thus 10.5-fold and 10.1-fold improvements are achieved in comparison with the traditional wavelength modulation spectroscopy.Meanwhile,their excellent R-square values reach 0.9983 and 0.99564 for the concentration ranges of 500 ppm to 2000 ppm and 800 ppm to 2700 ppm,respectively. | Zongliang WANG Jun CHANG Huishan YU Cunwei TIAN Hao ZHANG Xiukun ZHANG Longfei TANG Qinduan ZHANG Yiwen FENG | 2019 | Photonic Sensors2019，9，4： | 2 |
| 8 | Silencing myostatin gene by RNAi in sheep embryos显示文摘 | Dayun Tang Huabin Zhu Jianmin Wu Hanzhong Chen Yan Zhang Xueming Zhao Xiaoliang Chen Weihua Du Dong Wang Xiukun Lin | 2012 | Journal of Biotechnology2012，，3： | 2 |
| 9 | An efficient papaya leaf distortion mosaic potyvirus vector for virus-induced gene silencing in papaya显示文摘Papaya(Carica papaya L.)is regarded as an excellent model for genomic studies of tropical trees because of its short generation time and its small genome that has been sequenced.However,functional genomic studies in papaya depend on laborious genetic transformations because no rapid tools exist for this species.Here,we developed a highly efficient virus-induced gene silencing(VIGS)vector for use in papaya by modifying an artificially attenuated infectious clone of papaya leaf distortion mosaic virus(PLDMV;genus:Potyvirus),PLDMV-E,into a stable Nimble Cloning(NC)-based PLDMV vector,pPLDMV-NC,in Escherichia coli.The target fragments for gene silencing can easily be cloned into pPLDMV-NC without multiple digestion and ligation steps.Using this PLDMV VIGS system,we silenced and characterized five endogenous genes in papaya,including two common VIGS marker genes,namely,phytoene desaturase,Mg-chelatase H subunit,putative GIBBERELLIN(GA)-INSENSITIVE DWARF1A and 1B encoding GA receptors;and the cytochrome P450 gene CYP83B1,which encodes a key enzyme involved in benzylglucosinolate biosynthesis.The results demonstrate that our newly developed PLDMV VIGS vector is a rapid and convenient tool for functional genomic studies in papaya. | Decai Tuo Pu Yan Guangyuan Zhao Hongguang Cui Guopeng Zhu Yang Liu Xiukun Yang He Wang Xiaoying Li Wentao Shen Peng Zhou | 2021 | Horticulture Research2021，8，1： | 2 |
| 10 | Polymyxin resistance caused by large-scale genomic inversion due to IS26 intramolecular translocation in Klebsiella pneumoniae显示文摘Polymyxin B and polymyxin E(colistin)are presently considered the last line of defense against human infections caused by multidrug-resistant Gram-negative organisms such as carbapenemase-producer Enterobacterales,Acinetobacter baumannii,and Klebsiella pneumoniae.Yet resistance to this last-line drugs is a major public health threat and is rapidly increasing.Polymyxin S2(S2)is a polymyxin B analogue previously synthesized in our institute with obviously high antibacterial activity and lower toxicity than polymyxin B and colistin.To predict the possible resistant mechanism of S2for wide clinical application,we experimentally induced bacterial resistant mutants and studied the preliminary resistance mechanisms.Mut-S,a resistant mutant of K.pneumoniae ATCC BAA-2146(Kpn2146)induced by S2,was analyzed by whole genome sequencing,transcriptomics,mass spectrometry and complementation experiment.Surprisingly,large-scale genomic inversion(LSGI)of approximately 1.1 Mbp in the chromosome caused by IS26mediated intramolecular transposition was found in Mut-S,which led to mgrB truncation,lipid A modification and hence S2resistance.The resistance can be complemented by plasmid carrying intact mgrB.The same mechanism was also found in polymyxin B and colistin induced drug-resistant mutants of Kpn2146(Mut-B and Mut-E,respectively).This is the first report of polymyxin resistance caused by IS26 intramolecular transposition mediated mgrB truncation in chromosome in K.pneumoniae.The findings broaden our scope of knowledge for polymyxin resistance and enriched our understanding of how bacteria can manage to survive in the presence of antibiotics. | Haibin Li Lang Sun Han Qiao Zongti Sun Penghe Wang Chunyang Xie Xinxin Hu Tongying Nie Xinyi Yang Guoqing Li Youwen Zhang Xiukun Wang Zhuorong Li Jiandong Jiang Congran Li Xuefu You | 2023 | Acta Pharmaceutica Sinica B2023，13，9： | 1 |
| 11 | Bacterial diversity in the sediment of Crescent Moon Spring,Kumtag Desert,Northwest China显示文摘Desert lake, a unique oasis in desert ecosystems, harbours different bacterial communities. Thus, it is considered as a hub of bacterial diversity. In this study, bacterial diversity in the sediment of Crescent Moon Spring, Kumtag Desert, Northwest China was analyzed using high-throughput amplicon pyrosequencing analysis. The sequences of the most abundant OUTs(Operational Taxonomic Units) in the sediment of Crescent Moon Spring were compared with the sequences of those most abundant OUTs of various origins from NCBI Gen Bank database to detect the origins of bacteria in the sediment of Crescent Moon Spring. Also, bacterial compositions between sediment of Crescent Moon Spring and other desert and lake ecosystems(including desert lakes) worldwide were compared using cluster analysis to determine the possible factors affecting bacterial compositions. In total, 11,855 sequences were obtained and 30 phyla were identified. At the phylum level, the dominant phylum was Proteobacteria with α-Proteobacteria being the first dominant class and the second dominant phylum was Planctomycetes. Our finding that α-Proteobacteria being the first dominant class of Proteobacteria and Planctomycetes being the second dominant phyla are somewhat contradictory with reports from other desert lake sediments. This difference could be resulted from water hydration and conductivity, as well as oligotrophic conditions of Crescent Moon Spring. At the genus level, Rhodobacter, Caldilinea, Planctomyces, and Porphyrobacter were the dominant genera in the sediment of Crescent Moon Spring. Comparisons on sequences of the most abundant OUTs(including OTU3615, OTU6535, and OTU6646) between sediment of Crescent Moon Spring and various origins from NCBI Gen Bank database indicate that the origins of bacteria in the sediment of Crescent Moon Spring are likely from the underground water. Furthermore, cluster analysis on comparisons of bacteria compositions between sediment of Crescent Moon Spring and other desert and lake ecosystems(including desert lakes) worldwide shows that at regional scales, bacterial compositions may be mainly affected by geographical patterns, precipitation amounts, and p H values. Collectively, our results provide new knowledge on the bacterial diversity in desert lake ecosystems. | ZHANG Wei ZHANG Gaosen WU Xiukun LIU Guangxiu DONG Zhibao QU Jianjun WANG Yun CHEN Tuo | 2017 | Journal of Arid Land2017，9，2： | 1 |
| 12 | A hybrid method for XMLclustering by structure and content显示文摘 | Piao Yong Wang Xiukun | 2011 | J of Software2011，6，12： | 1 |
| 13 | Silencing myostatin gene by RNAi in sheep embryos显示文摘 | Dayun Tang Huabin Zhu Jianmin Wu Hanzhong Chen Yan Zhang Xueming Zhao Xiaoliang Chen Weihua Du Dong Wang Xiukun Lin | 2012 | Journal of Biotechnology2012，，3： | 1 |
| 14 | Discussion of liquid threshold pressure gradient显示文摘Some authors believe that a minimum pressure gradient(called threshold pressure gradient(TPG))is required before a liquid starts to flow in a porous medium.In a tight or shale oil formation,this TPG phenomenon becomes more important,as it is more difficult for a fluid to flow.In this paper,experimental data on TPG published in the literature are carefully reviewed.What we found is that a very low flow velocity corresponding to a very low pressure gradient cannot be measured in the experiments.Experiments can only be done above some measurable flow velocities.If these flow velocities and their corresponding pressure gradients are plotted in an XY plot and extrapolated to zero velocity,a non-zero pressure gradient corresponds to this zero velocity.This non-zero pressure gradient is called threshold pressure gradient in the literature.However,in the regime of very low velocity and very low pressure gradient,the data gradually approach to the origin of the plot,demonstrating a non-linear relationship between the pressure gradient and the velocity.But the data do not approach to a point of zero velocity and a threshold pressure gradient.Therefore,the concept of threshold pressure gradient is a result of data misinterpretation of available experimental data.The correct interpretation is that there are two flow regimes:nonlinear flow regime(non-Darcy flow regime)when the pressure gradients are low,and linear flow regime(Darcy flow regime)when the pressure gradient is intermediate or high.The nonlinear flow regime starts from the origin point.As the pressure gradient is increased,the curve becomes a straight line demonstrating the linear flow regime.We have verified our views by first analyzing the causes of non-Darcy flow,and then systematically analyzed typical experimental data and correlations in the literature.We conclude that TPG does not exist.We also use several counter examples to support our conclusion. | Xiukun Wang James J.Sheng | 2017 | Petroleum2017，3，2： | 1 |
| 15 | Chaotic behavior in fractional-order memristor-based simplest chaotic circuit using fourth degree polynomial显示文摘 | Lin Teng Herbert H. C. Iu Xingyuan Wang Xiukun Wang | 2014 | Nonlinear Dynamics . 2014 (1-2)2014，，： | 1 |
| 16 | Chaotic behavior in fractional-order memristor-based simplest chaotic circuit using fourth degree polynomial显示文摘 | Lin Teng Herbert H. C. Iu Xingyuan Wang Xiukun Wang | 2014 | Nonlinear Dynamics2014，，1： | 1 |
| 17 | Oppositionbased Cooperative Co-evolutionary Differential Evolution Algorithm with Gaussian Mutation for Simplified Satellite Module Optimization显示文摘 | Wang Yuanhui Wang Xiukun Teng Hongfei | 2012 | Information Technology Journal2012，11，1： | 1 |
| 18 | Human embryos in a dish - modeling early embryonic development with pluripotent stem cells显示文摘Stem cell-based embryo models present new opportunities to study early embryonic development.In a recent study,Kagawa et al.identified an approach to create human pluripotent stem cell-based blastoids that resemble the human blastocysts.These blastoids efficiently generated analogs of the EPI,TE,PrE lineages with transcriptomes highly similar to those found in vivo.Furthermore,the formation of these lineages followed the same sequence and pace of blas-tocyst development,and was also dependent on the same pathways required for lineage specification.Finally,the blastoids were capable of attaching to stimulated endometrial cells to mimic the process of implantation.While more comprehensive analysis is needed to confirm its validity and usefulness,this new blastoid system presents the latest development in the attempt to model early human embryogenesis in vitro. | Xiukun Wang Guang Hu | 2022 | Cell Regeneration2022，11，1： | 0 |
| 19 | Pore scale numerical investigation of counter-current spontaneous imbibition in multi-scaled pore networks显示文摘The multi-scaled pore networks of shale or tight reservoirs are considerably different from the conventional sandstone reservoirs.After hydraulic fracturing treatment,the spontaneous imbibition process plays an important role in the productivity of the horizontal wells.Applying the color-gradient model of Lattice Boltzmann Method(LBM)accelerated with parallel computing,we studied the countercurrent spontaneous imbibition process in two kinds of pore structures with different interlacing distributions of large and small pores.The effect of geometry configuration of pore arrays with different pore-scale and the capillary number Ca on the mechanism of counter-current spontaneous imbibition as well as the corresponding oil recovery factor are studied.We found that the wetting phase tends to invade the small pore array under small Ca in both types of geometry configurations of different pore arrays of four pore arrays zones.The wetting phase also tends to invade the pore array near the inlet for injecting the wetting phase no matter if it is a large pore array or small pore array except for the situation when the Ca is large to a certain value.In this situation,the small pore arrays show resistance to the wetting phase,so the wetting phase doesn't invade the small pore near the inlet,but invades the large pore preferentially.Both the geometry configurations of different pore arrays and Ca have a significant effect on the oil recovery factor.This work will help to solve the doubt about the selectivity of the multi-scaled pores of the wetting phase and the role of pores with different sizes in imbibition and oil draining in countercurrent spontaneous imbibition processes. | Yuchen Wu Xiukun Wang Chaofan Zhang Chenggang Xian | 2023 | Petroleum2023，9，4： | 0 |
| 20 | Evolution and development of potent monobactam sulfonate candidate IMBZ18g as a dual inhibitor against MDR Gram-negative bacteria producing ESBLs显示文摘A series of new monobactam sulfonates is continuously synthesized and evaluated for their antimicrobial efficacies against Gram-negative bacteria.Compound 33a(IMBZ18G)is highly effective in vitro and in vivo against clinically intractable multi-drug-resistant(MDR)Gram-negative strains,with a highly druglike nature.The checkerboard assay reveals its significant synergistic effect withβ-lactamase inhibitor avibactam,and the MIC values against MDR enterobacteria were reduced up to 4—512folds.X-ray co-crystal and chemoproteomic assays indicate that the anti-MDR bacteria effect of 33a results from the dual inhibition of the common PBP3 and some class A and Cβ-lactamases.Accordingly,preclinical studies of 33a alone and 33a-avibactam combination as potential innovative candidates are actively going on,in the treatment ofβ-lactamase-producing MDR Gram-negative bacterial infections. | Zhiwen Li Zhihao Guo Xi Lu Xican Ma Xiukun Wang Rui Zhang Xinxin Hu Yanxiang Wang Jing Pang Tianyun Fan Yonghua Liu Sheng Tang Haigen Fu Jingpu Zhang Yinghong Li Xuefu You Danqing Song | 2023 | Acta Pharmaceutica Sinica B2023，13，7： | 0 |
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