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| 1 | Characterization and Fine Mapping of a Novel Rice Narrow Leaf Mutant nal9显示文摘A narrow leaf mutant was isolated from transgenic rice(Oryza sativa L.)lines carrying a T-DNA insertion.The mutant is characterized by narrow leaves during its whole growth period,and was named nal9(narrow leaf 9).The mutant also has other phenotypes,such as light green leaves at the seedling stage,reduced plant height,a small panicle and increased tillering.Genetic analysis revealed that the mutation is controlled by a single recessive gene.A hygromycin resistance assay showed that the mutation was not caused by T-DNA insertion,so a map-based cloning strategy was employed to isolate the nal9 gene.The mutant individuals from the F2generations of a cross between the nal9 mutant and Longtepu were used for mapping.With 24 F2mutants,the nal9 gene was preliminarily mapped near the marker RM156 on the chromosome 3.New INDEL markers were then designed based on the sequence differences between japonica and indica at the region near RM156.The nal9 gene was fnally located in a 69.3 kb region between the markers V239B and V239G within BAC OJ1212_C05 by chromosome walking.Sequence and expression analysis showed that an ATP-dependent Clp protease proteolytic subunit gene(ClpP)was most likely to be the nal9 gene.Furthermore,the nal9 mutation was rescued by transformation of the ClpP cDNA driven by the 35S promoter.Accordingly,the ClpP gene was identifed as the NAL9 gene.Our results provide a basis for functional studies of NAL9 in future work. | Wei Li Chao Wu Guocheng Hu Li Xing Wenjing Qian Huamin Si Zongxiu Sun Xingchun Wang Yaping Fu Wenzhen Liu | 2013 | Journal of Integrative Plant Biology2013，55，11： | 8 |
| 2 | nvolvement of collagen-binding heat shock protein 47 in scleroderma-associated fibrosis显示文摘 | Haiyan Chu Ting Wu Wenyu Wu Wenzhen Tu Shuai Jiang Sidi Chen Yanyun Ma Qingmei Liu Xiaodong Zhoua Li Jin Jiucun Wang | 2015 | Protein & Cell2015，6，8： | 5 |
| 3 | Numerical Simulation of Shrinkage Cavity Formation in Spheroidal Graphite Iron Castings Based on Micro Modeling显示文摘A micro-modeling method (MM) for the quantitative prediction of the shrinkage cavity formation in SGiron castings is proposed. The mathematical models describing the volume changes during the solidification ofspheroidal graphite cast iron are established based on the models of solidification kinetics. The shrinkage cavityformation of T-shaped SG iron castings is calculated with MM method. The calculated results are compared with theexperimental results. It is shown that the predicted size, shape and distribution of shrinkage cavity by MM methodare in good agreement with the measured results. | Wenzhen Li Baicheng Liu Shantong Jin (Metallurgy Engineering School, University of Science and Technology Beijing, Beijing 100083, China)(Department of Mechanical Engineering, Tsinghua University, Beijing 100084, China) | 1998 | International Journal of Minerals,Metallurgy and Materials1998，12，1： | 5 |
| 4 | High-Performance Solid-State Supercapacitors Fabricated by Pencil Drawing and Polypyrrole Depositing on Paper Substrate显示文摘A solid-state powerful supercapacitor(SC) is fabricated with a substrate of Xerox paper. Its current collector based on a foldable electronic circuit is developed by simply pencil drawing. Thin graphite sheets on paper provide effective channels for electron transmission with a low resistance of 95 X sq-1. The conductive organic material of polypyrrole coated on thin graphite sheets acts as the electrode material of the device. The as-fabricated SC exhibits a high specific capacitance of 52.9 F cm-3at a scan rate of 1 m V s-1. An energy storage unit fabricated by three full-charged series SCs can drive a commercial light-emitting diode robustly. This work demonstrated a simple, versatile and costeffective method for paper-based devices. | Jiayou Tao Wenzhen Ma Nishuang Liu Xiaoliang Ren Yuling Shi Jun Su Yihua Gao | 2015 | Nano-Micro Letters2015，7，3： | 4 |
| 5 | In-flight observations of electromagnetic interferences emitted by satellite显示文摘Using the data from STAFF/TC-1, this paper for the first time analyzes the electromagnetic interferences of Chinese scientific satellite. The electromagnetic interference of satellite exists mainly below 30 Hz, but can extend to 190 Hz with an obviously decreasing power spectral density. The electromagnetic interferences at frequencies below 190 Hz have good correlation with the solar aspect angle. The electromagnetic interferences at frequencies between 190 and 830 Hz have also correlation with solar as-pect angle. However, the electromagnetic interferences at frequencies above 830 Hz have no correlation with the solar aspect angle. The correlation coefficient between solar aspect angel and electromagnetic interferences is around 0.90. The larger the solar aspect angle, the stronger the satellite electromagnetic interference. When the solar aspect angle increases from 90.6° to 93.6°, the electromagnetic interferences at frequencies <10 Hz increase by 8 times and those at frequencies 190―830 Hz increase by 60%. This close association of electromagnetic interferences with the solar aspect angle indicates that the solar aspect angle is the main factor to determine the electromagnetic interferences. The electromagnetic interferences of satellite in sunlight are larger than those in eclipse. The electro-magnetic interference produced by solar panel occupies about 87% in the low frequency band (<100 Hz) and 94% in the high frequency band (>100 Hz) of the total electromagnetic interference produced by satellite. These in flight observations of electromagnetic radiation of satellites will be very helpful to the designs of future satellites of space sciences or earthquake sciences. | CAO JinBin YANG JunYing YUAN ShiGan SHEN XuHui LIU YuanMo YAN ChunXiao LI WenZhen CHEN Tao | 2009 | Science China(Technological Sciences)2009，52，7： | 4 |
| 6 | Increasing the Genetic Recombination Frequency by Partial Loss of Function of the Synaptonemal Complex in Rice显示文摘 | Wang, Kejian Wang, Chun Liu, Qing Liu, Wenzhen Fu, Yaping | 2015 | Molecular Plant2015，8，8： | 4 |
| 7 | Clinical assessment and genomic landscape of a consanguineous family with three Kallmann syndrome descendants显示文摘尽管引起 Kallmann 症候群(KS ) 的一些基因被传统的连接分析和候选人基因技术识别了,症候群是在遗体糟糕理解的病人的多数的分子的病原学。在这份报纸,我们在场对与三个 KS 后代一起的一个同宗的汉中国家庭的临床的评价。从一个染色体宽的观点理解 KS 的分子的病原学,我们用 Affymetrix 染色体宽的人的 SNP 数组在这个家庭调查了结构的变化的染色体宽的侧面 6.0 站台。结果表明三个影响个人在染色体 1p21.1, 2q32.2, 8q21.13, 14q21.2 和 Xp22.31 上有普通拷贝数字变体(microdeletions ) 。而且, Xp22.31 上的拷贝数字变体位于 KAL1 的 intron,它引起 X 连接 KS。二 PCR 试金在这些区域上被执行验证用薄片获得的结果。另外,在这个区域的 genomic microdeletions 在 29 个汉中国分散的 KS 案例之一和四个另外的家庭案例之一中,然而并非用 26 汉汉语被验证分散的 normosmic 自发的 hypogonadotropic 性腺机能减退案例和 100 无关的汉汉语正常控制。我们的结果提供新奇卓见进对 KS 的分子的病原学的某些拷贝数字变体的相对贡献并且为进一步的研究产生有趣的候选人区域的一张表。 | Shilin Zhang Yanping Tang Tao Wang Jun Yang Ke Rao Lingyun Zhao Wenzhen Zhu Xianghu Meng Shaogang Wang Jihong Liu Weimin Yang Zhangqun Ye | 2011 | Asian Journal of Andrology2011，13，1： | 3 |
| 8 | A potent PGK1 antagonist reveals PGK1 regulates the production of IL-1βand IL-6显示文摘Glycolytic metabolism enzymes have been implicated in the immunometabolism field through changes in metabolic status. PGK1 is a catalytic enzyme in the glycolytic pathway. Here, we set up a high-throughput screen platform to identify PGK1 inhibitors. DC-PGKI is an ATPcompetitive inhibitor of PGK1 with an affinity of Kd= 99.08 nmol/L. DC-PGKI stabilizes PGK1in vitro and in vivo, and suppresses both glycolytic activity and the kinase function of PGK1. In addition,DC-PGKI unveils that PGK1 regulates production of IL-1β and IL-6 in LPS-stimulated macrophages.Mechanistically, inhibition of PGK1 with DC-PGKI results in NRF2(nuclear factor-erythroid factor 2-related factor 2, NFE2L2) accumulation, then NRF2 translocates to the nucleus and binds to the proximity region of Il-1β and Il-6 genes, and inhibits LPS-induced expression of these genes. DC-PGKI ameliorates colitis in the dextran sulfate sodium(DSS)-induced colitis mouse model. These data support PGK1 as a regulator of macrophages and suggest potential utility of PGK1 inhibitors in the treatment of inflammatory bowel disease. | Liping Liao Wenzhen Dang Tingting Lin Jinghua Yu Tonghai Liu Wen Li Senhao Xiao Lei Feng Jing Huang Rong Fu Jiacheng Li Liping Liu Mingchen Wang Hongru Tao Hualiang Jiang Kaixian Chen Xingxing Diao Bing Zhou Xiaoyan Shen Cheng Luo | 2022 | Acta Pharmaceutica Sinica B2022，12，11： | 2 |
| 9 | Deoxyribozymes inhibit the expression of periodl gene in vitro显示文摘To investigate the effect of two deoxyribozymes targeting period1 (per1) mRNA in vitro for exploring a novel gene therapy approach about circadian rhythm diseases, the specific deoxyribozymes targeting per1 were designed and synthesized chemically following MFold analysis according to its mRNA secondary structure. per1 RNA fragments were prepared by in vitro transcription of pcDNA3.1(+)-per1164:256. The cleavage reactions containing deoxyribozymes and per1 RNA fragments were performed under certain conditions. With the transfection tech- nique mediated by LipofectAMINETM, pcDNA3-per1 and DRz164 or DRz256 were introduced into NIH3T3 cells. The effects of deoxyribozymes on per1 were studied by reverse tran- script-polymerase chain reaction (RT-PCR) and flow cytometry (FCM). When deoxyribozymes and RNA transcripts were incubated under the adopted conditions at 37℃ for 2 h, about 63% of per1164:256 RNA transcripts were cleaved by DRz164 and about 50.5% by DRz256. After co- transfecting pcDNA3-per1 with DRz164 or DRz256, the expression of per1 mRNA was de- creased, as indicated by RT-PCR semi-quantity analysis. FCM analysis showed that Per1 protein was inhibited. Both DRz164 and DRz256 targeting per1 have the specific cleavage activity to- ward per1 mRNA in vitro and can highly block the expression of per1 gene in cellular milieu. | ZHOU Wei WANG Yueqi LIU Yanyou PENG Wenzhen XIAO Jing ZHU Bin WANG Zhengrong | 2005 | Science China(Life Sciences)2005，48，3： | 1 |
| 10 | Cortical and Subcortical Grey Matter Abnormalities in White Matter Hyperintensities and Subsequent Cognitive Impairment显示文摘Grey matter(GM)alterations may contribute to cognitive decline in individuals with white matter hyperintensities(WMH)but no consensus has yet emerged.Here,we investigated cortical thickness and grey matter volume in 23 WMH patients with mild cognitive impairment(WMH-MCI),43 WMH patients without cognitive impairment,and 55 healthy controls.Both WMH groups showed GM atrophy in the bilateral thalamus,fronto-insular cortices,and several parietal-temporal regions,and the WMH-MCI group showed more extensive and severe GM atrophy.The GM atrophy in the thalamus and fronto-insular cortices was associated with cognitive decline in the WMH-MCI patients and may mediate the relationship between WMH and cognition in WMH patients.Furthermore,the main results were well replicated in an independent dataset from the Alzheimer's Disease Neuroimaging Initiative database and in other control analyses.These comprehensive results provide robust evidence of specific GM alterations underlying WMH and subsequent cognitive impairment. | Wenhao Zhu Hao Huang Shiqi Yang Xiang Luo Wenzhen Zhu Shabei Xu Qi Meng Chengchao Zuo Yong Liu Wei Wang Alzheimer’s Disease Neuroimaging Initiative | 2021 | Neuroscience Bulletin2021，37，6： | 1 |
| 11 | Astragaloside IV prevents acute kidney injury in two rodent models by inhibiting oxidative stress and apoptosis pathways显示文摘 | Dingkun Gui Jianhua Huang Wei Liu Yongping Guo Wenzhen Xiao Niansong Wang | 2013 | Apoptosis2013，，4： | 1 |
| 12 | Shrinkage Behaviour of Spheroidal Graphite Cast Iron in Green and Dry Sand Molds for the Benchmarking of Solidification Simulation显示文摘The effects of metallurgical and processing parameters on the formation of shrinkage cavities and porosities in spheroidal graphite cast iron have been studied, considering the parameters of carbon equivalent, inoculation, casting modulus, mold type (green or dry) and pouring temperature within specific ranges of these variables. Based on the orthogonal experiments, the metallurgical and processing parameters of the minimum casting shrinkage and the maximum casting shrinkage were obtained, and the effects of metallurgical and processing parameters on the formation of shrinkage cavities and porosities in spheroidal graphite cast iron castings were discussed. Finally, two regression equations relating these variables to the formation of shrinkage porosity were derived based upon the orthogonal experiments conducted. | Wenzhen LI and Baicheng LIU Department of Mechanical Engineering, Tsinghua University, Beijing 100084, China Jiarong LI Beijing Institute of Aeronautical Materials, Beijing 100095, China | 2001 | Journal of Materials Science & Technology2001，17，6： | 1 |
| 13 | The pressure melting of ice around a horizontal elliptical cylinder显示文摘 | Wenzhen Chen Haofeng Li Ming Gao Zhen Liu Fengrui Sun | 2005 | Heat and Mass Transfer2005，，2： | 1 |
| 14 | Structure characterization of a novel polysaccharide from dictyophora indusiata and its macrophage immunomodulatory activities显示文摘 | LIAO Wenzhen LUO Zhen LIU Dan | 2015 | Journal of Agricultural and Food Chemistry2015，63，2： | 1 |
| 15 | Beyond the Green Revolution: Improving crop productivity and sustainability by modulating plant growth-metabolic coordination显示文摘Rice is one of the most important staple food for over half the world's population.New evidence from the Food and Agriculture Organization shows that the number of hungry people in the world is growing,reaching more than 690 million in 2020.It is an urgent task to increase crop production to feed the people suffering from chronic hunger in the world.The Green Revolution of the 1960s boosted cereal yields through widespread adoption of semi-dwarf varieties and extensive use of inorganic fertilizers(Khush,2001).Today,the most productive Green Revolution varieties(GRVs)of rice and wheat in the world retain semi-dwarf phenotypes,which have several advantages over tall traditional varieties:(1)they have a lower center of gravity of the plant body,which decreases the risk of lodging and allows farmers to apply excessive fertilizers to obtain high yield;(2)they have an increased harvest index,which enables plants to allocate more energy and resources to seed production。 | Qian Liu Kun Wu Yunzhe Wu Wenzhen Song Shuoxun Wang Xiangdong Fu | 2022 | Molecular Plant2022，15，4： | 1 |
| 16 | Modulating the C-terminus of DEP1 synergistically enhances grain quality and yield in rice显示文摘Grain shape is a key breeding target that influences both grain yield and appearance quality in rice. Breeding for long-slender grains is an effective strategy to improve grain quality (Jain et al., 2004;Wang et al., 2012, 2015a). In the past two decades, several quantitative trait loci (QTLs) responsible for grain size and shape have been identified, including GS3 (Fan et al., 2006), GS5 (Li et al., 2011). | Haixiang Huang Yafeng Ye Wenzhen Song Qi Li Ruixi Han Chenchen Wu Shuoxun Wang Jianping Yu Xueying Liu Xiangdong Fu Qian Liu Kun Wu | 2022 | Journal of Genetics and Genomics2022，49，5： | 1 |
| 17 | Geant4 simulation of plastic scintillators for a prototype μSR spectrometer显示文摘The experimental muon source on China Spallation Neutron Source(CSNS) is expected to be a high intensity(105μ+/s) surface muon source with a small beam spot of 4-cm diameter.For a practical application of this muon source,we are devoting to develop the first pulsed μSR spectrometer in China.In this paper,the performance of plastic scintillators in the μSR spectrometer is studied by Monte Carlo simulation.The processes such as positron energy deposition,scintillation photons production,light propagation and photon-electron conversion are carefully considered.According to the results,an optimal dimension of the plastic scintillator is proposed using for our future spectrometer,which has a long-strip shape with the dimension variation range of 50–60 mm length,5–8 mm height,and 10–12 mm width.Finally,we can build a spectrometer with a count rate up to 104e+/s by 100–120 forward and backward segmental detectors in total.The simulation could serve as an important guide for spectrometer construction. | XU Wenzhen LIU Yanfen TAN Zongquan XIAO Ran KONG Wei YE Bangjiao | 2013 | Nuclear Science and Techniques2013，24，4： | 1 |
| 18 | Effect of nitrogen and phosphorus application on soil nitrogen morphological characteristics and grain yield of oil flax显示文摘In order to identify effects of nitrogen(N)and phosphorus(P)on soil nitrogen morphological characteristics and grain yield of oil flax,a two-factor experiment was conducted in a randomized complete block design in typical semi-arid and hilly-gully area of Loess Plateau with 3 replicates in 2013 and 2014.Two levels of N application included 150 kg/hm2(N2)and 75 kg/hm2(N1).P application included 150 kg P2 O5/hm2(P2)and 75 kg P2 O5/hm2(P1).Temporal and spatial variation of soil nitrate nitrogen(NO3-N)and ammonium nitrogen(NH4+-N)contents in 0-60 cm soil layer,and relationship between soil NO3-N accumulation(SNA)and grain yield of oil flax were analyzed.Results showed that SNA increased with evaluated N application rate in different soil layers(0-20 cm,20-40 cm and 40-60 cm).With the increased P application,SNA increased at N1 level but decreased at N2 level.SNA under N2 P1 treatment increased by 73.33%in 2013 and 74.97%in 2014 respectively,compared with control treatment(CK)at maturity stage.Grain yield of oil flax also increased by 44.27%in 2013 and56.55%in 2014,compared with CK under the same treatment.Correlation analysis showed that SNA in different soil layers were respectively positively correlated with grain yield.In conclusion,this research suggested that the optimal fertilizer application rate was 150 kg N/hm2 and 75 kg P2 O5/hm2 in the Northwest of China. | Dong Liu Zhengjun Cui Bin Yan Yuhong Gao Bin Wu Wenzhen Li Junyi Niu | 2020 | Oil Crop Science2020，5，2： | 0 |
| 19 | GB7 acetate,a galbulimima alkaloid from Galbulimima belgraveana,possesses anticancer effects in colorectal cancer cells显示文摘GB7 acetate is a galbulimima alkaloid obtained from Galbulimima belgraveana.However,information regarding its structure,biological activities,and related mechanisms is not entirely available.A series of spectroscopic analyses,structural degradation,interconversion,and crystallography were performed to identify the structure of GB7 acetate.The MTT assay was applied to measure cell proliferation on human colorectal cancer HCT 116 cells.The expressions of the related proteins were measured by Western blotting.Transmission electron microscopy(TEM),acridine orange(AO)and monodansylcadaverine(MDC)staining were used to detect the presence of autophagic vesicles and autolysosomes.A transwell assay was performed to demonstrate metastatic capabilities.Oxygen consumption rate(OCR)and extracellular acidification rate(ECAR)assays were performed to determine the mitochondrial oxidative phosphorylation(OXPHOS)and glycolysis activity of HCT 116 cells.The data showed that GB7 acetate suppressed the proliferation and colony-forming ability of HCT 116 cells.Pretreatment with GB7 acetate significantly induced the formation of autophagic vesicles and autolysosomes.GB7 acetate upregulated the expressions of LC3 and Thr172 phosphorylated adenosine 5'-monophosphate(AMP)-activated protein kinase a(p-AMPKa),which are key elements of autophagy.In addition,GB7 acetate suppressed the metastatic capabilities of HCT 116 cells.Additionally,the production of matrix metallo-proteinase-2(MMP-2)and MMP-9 was reduced,whereas the expression of E-cadherin(E-cad)was upregulated.Furthermore,GB7 acetate significantly reduced mitochondrial OXPHOS and glycolysis.In conclusion,the structure of the novel Galbulimima alkaloid GB7 acetate was identified.GB7 acetate was shown to have anti-proliferative,pro-autophagic,anti-metastatic,and anti-metabolite capabilities in HCT 116 cells.This study might provide new insights into cancer treatment efficacy and cancer chemoprevention. | Ziyin Li Lianzhi Mao Bin Yu Huahuan Liu Qiuyu Zhang Zhongbo Bian Xudong Zhang Wenzhen Liao Suxia Sun | 2022 | Journal of Pharmaceutical Analysis2022，12，2： | 0 |
| 20 | Mitochondria determine the sequential propagation of the calcium macrodomains revealed by the super-resolution calcium lantern imaging显示文摘Despite the wide application of super-resolution(SR)microscopy in biological studies of cells,the technology is rarely used to monitor functional changes in live cells.By combining fast spinning disc-confocal structured illumination microscopy(SD-SIM)with loading of cytosolic fluorescent Ca2+indicators,we have developed an SR method for visualization of regional Ca2+dynamics and related cellular organelle morphology and dynamics,termed SR calcium lantern imaging.In COS-7 cells stimulated with ATP,we have identified various calcium macrodomains characterized by different types of Ca2+release from endoplasmic reticulum(ER)stores.Finally,we demonstrated various roles of mitochondria in mediating calcium signals from different sources;while mitochondria can globally potentiate the Ca2+entry associated with store release,mitochondria also locally control Ca2+release from the neighboring ER stores and assist in their refilling processes. | Yulin Zhang Jianyong Wang Shijia Xing Liuju Li Shiqun Zhao Wenzhen Zhu Kuo Liang Yanmei Liu Liangyi Chen | 2020 | Science China(Life Sciences)2020，63，10： | 0 |
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