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| 1 | 水稻窄叶突变体nal10的鉴定与基因精细定位显示文摘利用EMS诱变粳稻品种武运粳7号获得一个新窄叶突变体nal10,该突变体在苗期表现出极窄叶和弱小的特征,在生殖期表现出矮化、窄叶、畸颖和不育的表型。组织解剖学分析表明,nal10的叶片窄化可能是由于叶片大脉和小脉数量减少造成的。遗传分析表明,该窄叶表型受一对隐性核基因控制。利用nal10与台中本地一号(TN1)构建的F2群体,通过混合分离法,在第1染色体上找到3个紧密连锁的SSR标记(RM1287、RM562和RM5638)。进一步利用新发展的分子标记,最终将该基因定位在STS标记PK83和PK78之间的55.2kb范围内,该区间共有8个开放阅读框。RT-PCR分析表明,NAL10的突变能够造成生长素生物合成、信号转导和运输基因转录水平的下降,因此NAL10是一个与生长素相关的窄叶新基因。这些结果为进一步克隆该基因、丰富水稻叶发育的遗传调控网络打下了基础。 | 方云霞 朱丽 潘江杰 余海平 薛大伟 饶玉春 王小虎 石珍源 张栋 祝阳舟 董国军 曾大力 张光恒 郭龙彪 胡江 钱前 | 2015 | 中国水稻科学2015，29，6： | 5 |
| 2 | 水稻叶色突变基因的遗传研究进展显示文摘介绍了水稻叶色突变体的类型及来源,综述了水稻叶色突变相关基因的发掘、定位和克隆进展情况,从细胞核遗传的突变、细胞质遗传的突变以及核质互作的突变等方面详细阐述了水稻叶色突变的遗传机理,并对其作为标记性状在育种上的应用提出了展望,旨在为水稻叶色突变体的研究与应用提供参考。 | 方希林 王悦 朱敏敏 邓跃军 张晶 李恩宇 | 2016 | 北方水稻2016，46，6： | 4 |
| 3 | 水稻叶形控制基因的挖掘及分析显示文摘叶片的形态结构和生理功能与水稻的产量关系密切,水稻叶片形态包括叶长、叶宽、叶面积、叶周长、叶厚、叶片卷曲度、叶倾角等性状.目前通过对水稻分离群体和突变体的深入研究,已定位和克隆到较多的叶形控制基因或数量性状位点(QTLs).根据水稻叶形控制基因或QTLs的最新研究进展情况,着重从叶长、叶宽、叶面积和叶卷曲度等性状,对已克隆叶形基因和QTLs的功能进行梳理和总结,以期为挖掘水稻叶形控制基因和理想株型育种提供参考,为进一步提高水稻的产量奠定理论基础. | 饶玉春 胡娟 金哲伦 胡锦春 邓琳杰 周纯 叶珺 | 2019 | 浙江师范大学学报(自然科学版)2019，42，3： | 3 |
| 4 | Development of Rice Leaves:How Histocytes Modulate Leaf Polarity Establishment显示文摘An ideal leaf shape is beneficial to the yield of rice.Molecular understanding of the leaf primordia and polarity establishment plays a significant role in exploring the genetic regulatory network of leaf morphogenesis.In recent years,researchers have cloned an array of coding genes and a few non-coding small RNAs involved in rice leaf development through regulating the development of leaf primordia,vascular bundles,sclerenchyma cells,bulliform cells,cell walls and epidermis cells.These genes and their interactions play critical roles in rice leaf development through the determination and regulatory role in gene expression,and their coordination with other genetic networks or signal pathways.But the relationship among these genes is poorly defined and the underlying network is still unclear.In this review,we introduced the regulatory pathways of leaf primordium development and leaf polarity establishment,mainly the relationship between cell development mechanism and leaf polarity establishment,focusing on how leaf tissue affects leaf shape.Hopefully,the regulation network reviewed here has immediate implications for future research and genomic design breeding. | Wang Jiajia Xu Jing Qian Qian Zhang Guangheng | 2020 | Rice science2020，27，6： | 2 |
| 5 | 水稻窄叶突变体nl(t)的遗传分析与基因定位显示文摘研究将小粒野生稻基因组DNA通过'穗茎注射法'导入恢复系RH78后产生变异,得到突变体材料nl(t)。遗传分析表明,突变体窄叶性状是由一对隐性核基因控制的,利用分子标记将nl(t)基因定位在水稻第4号染色体长臂上RM5511和RM3843标记之间,物理距离约570 kb的范围内。为进一步研究nl(t)的功能提供参考。 | 潘银林 毛毕刚 胡远艺 郭李勤 彭彦 韶也 阳和华 赵炳然 | 2015 | 生物学杂志2015，32，5： | 2 |
| 6 | 水稻窄叶突变体nal7-2(t)的遗传分析和基因定位显示文摘叶是水稻进行光合作用的主要器官,其形态特征直接影响水稻的株型,进而影响产量的形成。本研究利用60Co-γ辐射诱变籼稻9311获得了1份叶片宽度发育异常的窄叶突变体:nal7-2(t)。农艺性状调查显示,与野生型9311相比,nal7-2(t)有效分蘖极显著增加,叶宽、株高、千粒重和结实率极显著降低。组织切片和显微观察发现,nal7-2(t)叶形变窄是由于大维管束和小维管束的数目减少。遗传分析结果表明,nal7-2(t)的表型受一对隐性核基因控制。基因定位结果表明,nal7-2(t)位于第7号染色体长臂约370kb范围内。 | 余艳欢 仲崇元 李有光 郭旻 | 2020 | 农村经济与科技2020，31，10： | 0 |
| 7 | Identification and Characterization of a Novel Yellow Leaf Mutant yl1 in Rice显示文摘Leaf-color mutants play an important role in the study of chlorophyll metabolism,chloroplast development,and photosynthesis system.In this study,the yellow leaf 1(yl1)rice mutant was identified from the ethyl methane sulfonate-treated mutant progeny of Lailong,a glutinous japonica rice landrace cultivated in Guizhou Province,China.Results showed that yl1 exhibited yellow leaves with decreased chlorophyll content throughout the growth period.Chloroplast development in the yl1 mutant was disrupted,and the grana lamellae was loosely packed and disordered.RNA sequencing and real-time quantitative polymerase chain reaction(qRT-PCR)analysis revealed that the chlorophyll synthesis-related genes OsCHLH,OsCHLM,OsCHLG,PORB,and YGL8,as well as the chloroplast development-related genes FtsZ,OsRpoTp,and RbcL,were down-regulated in the yl1 mutant.Genetic analysis revealed that the yellow leaf phenotype of yl1 was controlled by recessive nuclear gene.By employing the MutMap method,the mutation responsible for the phenotype was mapped to a 6.17 Mb region between 17.34 and 23.51 Mb on chromosome 3.Two non-synonymous single-nucleotide polymorphisms(SNPs)located in the gene locus LOC_Os03g31210 and LOC_Os03g36760 were detected in this region.The two SNPs were further confirmed by PCR and Sanger sequencing.The expression patterns of the two candidate genes indicated that LOC_Os03g36760 showed greater potential for functional verification.Subcellular protein localization revealed that the encoded product of LOC_Os03g36760 was localized in the nucleus,cytoplasm,and plasma membrane.These results will be useful for further characterization and cloning of the yl1 gene,and for research on the molecular mechanisms controlling biogenesis and chloroplast biochemical processes. | Xiaofang Zeng Guangzheng Li Nu’an Liu Yan Li Jianrong Li Xiaozhen Huang Degang Zhao | 2022 | Phyton-International Journal of Experimental Botany2022，91，11： | 0 |
| 8 | 高粱窄叶突变体nal1表型鉴定及转录组分析显示文摘为解析高粱窄叶的分子调控机制,挖掘影响高粱叶片发育的关键差异表达基因。利用0.1%EMS化学诱变野生型BTX623获得高粱窄叶突变体nal1(narrow leaf1),以野生型植株BTX623和窄叶突变体nal1为材料,对不同发育时期的叶片长、叶片宽、株高、穗长等性状进行表型分析。结果表明:与野生型BTX623的叶片相比,2叶1心时,窄叶突变体nal1的叶片开始变窄;开花期植株和成熟期植株叶片宽和叶片长差异极显著;成熟期窄叶突变体nal1穗较松散,但植株株高差异不显著。开花期剑叶转录组分析结果表明:高粱窄叶突变体nal1和野生型BTX623开花期剑叶共筛选到差异表达基因1 520个,通过KEGG、GO富集分析得出,功能注释基因显著富集在植物激素信号转导、玉米素生物合成、光合作用天线蛋白、次级代谢产物生物合成等通路上;进一步研究发现参与调控生长素和玉米素信号转导的差异表达基因有17个,参与调控玉米素生物合成的差异表达基因为7个,这些基因在窄叶突变体nal1中差异表达,直接影响生长素、玉米素的信号转导和玉米素的生物合成。因此推测突变体nal1通过调节生长素、玉米素的信号转导和玉米素的生物合成影响窄叶突变体nal1叶片的发育,进而突变体nal1出现叶片变窄的性状。 | 刚爽 张世睿 陆晓春 高梽鑫 李金红 | 2023 | 沈阳农业大学学报2023，54，2： | 0 |
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