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| 1 | A 4-bp Insertion at ZmPLA 1 Encoding a Putative Phospholipase A Generates Haploid Induction in Maize显示文摘 | Chenxu Liu Xiang Li Dexuan Meng Yu Zhong Chen Chen Xin Dong Xiaowei Xu Baojian Chen Wei Li Liang Li Xiaolong Tian Haiming Zhao Weibin Song Haishan Luo Qinghua Zhang Jinsheng Lai Weiwei Jin Jianbing Yan Shaojiang Chen | 2017 | Molecular Plant2017，10，3： | 39 |
| 2 | Genetic dissection of maize seedling root system architecture traits using an ultra-high density bin-map and a recombinant inbred line population显示文摘Maize(Zea mays) root system architecture(RSA)mediates the key functions of plant anchorage and acquisition of nutrients and water. In this study,a set of 204 recombinant inbred lines(RILs) was derived from the widely adapted Chinese hybrid ZD958(Zheng58 Chang7-2),genotyped by sequencing(GBS) and evaluated as seedlings for 24 RSA related traits divided into primary,seminal and total root classes. Signi ficant differences between the means of the parental phenotypes were detected for 18 traits,and extensive transgressive segregation in the RIL population was observed for all traits. Moderate to strong relationships among the traits were discovered. A total of 62 quantitative trait loci(QTL) were identi fied that individually explained from1.6% to 11.6%(total root dry weight/total seedling shoot dry weight) of the phenotypic variation. Eighteen,24 and 20 QTL were identi fied for primary,seminal and total root classes of traits,respectively. We found hotspots of 5,3,4 and 12 QTL in maize chromosome bins 2.06,3.02-03,9.02-04,and 9.05-06,respectively,implicating the presence of root gene clusters or pleiotropic effects. These results characterized the phenotypic variation and genetic architecture of seedling RSA in a population derived from a successful maize hybrid. | Weibin Song Baobao Wang Andrew L Hauck Xiaomei Dong Jieping Li Jinsheng Lai | 2016 | Journal of Integrative Plant Biology2016，58，3： | 13 |
| 3 | Efficiency and Inheritance of Targeted Mutagenesis in Maize Using CRISPR-Cas9显示文摘CRISPR/Cas(clustered regularly interspaced short palindromic repeats/CRISPR-associated proteins) is an adaptive immune system in bacteria and archaea to defend against invasion from foreign DNA fragments. Recently, it has been developed as a powerful targeted genome editing tool for a wide variety of species. However, its application in maize has only been tested with transiently expressed somatic cells or with a limited number of stable transgenic T0 plants. The exact efficiency and specificity of the CRISPR/Cas system in the highly complex maize genome has not been documented yet. Here we report an extensive study of the well-studied type II CRISPR-Cas9 system for targeted genome editing in maize, with the codon-optimized Cas9 protein and the short non-coding guide RNA generated through a functional maize U6 sn RNA promoter. Targeted gene mutagenesis was detected for 90 loci by maize protoplast assay, with an average cleavage efficiency of 10.67%. Stable knockout transformants for maize phytoene synthase gene(PSY1) were obtained. Mutations occurred in germ cells can be stably inherited to the next generation. Moreover, no off-target effect was detected at the computationally predicted putative off-target loci. No significant difference between the transcriptomes of the Cas9 expressed and non-expressed lines was detected. Our results confirmed that the CRISPR-Cas9 could be successfully applied as a robust targeted genome editing system in maize. | Jinjie Zhu Ning Song Silong Sun Weilong Yang Haiming Zhao Weibin Song Jinsheng Lai | 2016 | Journal of Genetics and Genomics2016，43，1： | 12 |
| 4 | The RppC-AvrRppC NLR-effector interaction mediates the resistance to southern corn rust in maize显示文摘Southern corn rust(SCR),caused by the fungal pathogen Puccinia polysora,is a major threat to maize pro-duction worldwide.Efficient breeding and deployment of resistant hybrids are key to achieving durable control of SCR.Here,we report the molecular cloning and characterization of RppC,which encodes an NLR-type immune receptor and is responsible for a major SCR resistance quantitative trait locus.Further-more,we identified the corresponding avirulence effector,AvrRppC,which is secreted by P.polysora and triggers RppC-mediated resistance.Allelic variation of AvrRppC directly determines the effectiveness of RppC-mediated resistance,indicating that monitoring of AvrRppC variants in the field can guide the rational deployment of RppC-containing hybrids in maize production.Currently,RppC is the most frequently deployed SCR resistance gene in China,and a better understanding of its mode of action is crit-ical for extending its durability. | Ce Deng April Leonard James Cahill Meng Lv Yurong Li Shawn Thatcher Xueying Li Xiaodi Zhao Wenjie Du Zheng Li Huimin Li Victor Llaca Kevin Fengler Lisa Marshall Charlotte Harris Girma Tabor Zhimin Li Zhiqiang Tian Qinghua Yang Yanhui Chen Jihua Tang Xintao Wang Junjie Hao Jianbing Yan Zhibing Lai Xiaohong Fei Weibin Song Jinsheng Lai Xuecai Zhang Guoping Shu Yibo Wang Yuxiao Chang Weiling Zhu Wei Xiong Juan Sun Bailin Li Junqiang Ding | 2022 | Molecular Plant2022，15，5： | 6 |
| 5 | Defective Kernel 39 encodes a PPR protein required for seed development in maize显示文摘RNA editing is a posttranscriptional process that is important in mitochondria and plastids of higher plants. All RNA editing-specific trans-factors reported so far belong to PLS-class of pentatricopeptide repeat(PPR)proteins. Here, we report the map-based cloning and molecular characterization of a defective kernel mutant dek39 in maize. Loss of Dek39 function leads to delayed embryogenesis and endosperm development, reduced kernel size, and seedling lethality. Dek39 encodes an E subclass PPR protein that targets to both mitochondria and chloroplasts, and is involved in RNA editing in mitochondrial NADH dehydrogenase3(nad3) at nad3-247 and nad3-275. C-to-U editing of nad3-275 is not conserved and even lost in Arabidopsis, consistent with the idea that no close DEK39 homologs are present in Arabidopsis. However, the amino acids generated by editing nad3-247 and nad3-275 are highly conserved in many other plant species, and the reductions of editing at these two sites decrease the activity of mitochondria NADH dehydrogenase complex I,indicating that the alteration of amino acid sequence is necessary for Nad3 function. Our results indicate that Dek39 encodes an E sub-class PPR protein that is involved in RNA editing of multiple sites and is necessary for seed development of maize. | Xiaojie Li Wei Gu Silong Sun Zongliang Chen Jing Chen Weibin Song Haiming Zhao Jinsheng Lai | 2018 | Journal of Integrative Plant Biology2018，60，1： | 6 |
| 6 | Staggered Grid Scheme for the FFT-Based Methods显示文摘A staggered grid scheme is proposed to reduce both the total memory requirement and the CPU time of generating the corrected near matrix in the FFT- based methods. Two sets of Cartesian grids are used to project the source points and the field points, respectively. The proposed method does not lower the efficiency of computing far matrix-vector products, compared with the traditional uniform Cartesian grid scheme. Some numerical experiments are provided to demonstrate both the correctness and the efficiency of the proposed method. | XIE Jiaye KONG Weibin PANG Lili SONG Weiju HUANG Zhixiang WU Xianliang | 2019 | Chinese Journal of Electronics2019，28，5： | 4 |
| 7 | Maize WI5 encodes an endo-1,4-β-xylanase required for secondary cell wall synthesis and water transport in xylem显示文摘Water transport from roots to leaves through xylem is important for plant growth and development.Defects in water transport can cause drought stress,even when there is adequate water in the soil.Here,we identi-fied the maize(Zea mays)wilty5(wi5)mutant,which ex-hibits marked dwarfing and leaf wilting throughout most of its life cycle under normal growth conditions.wilty5 seed-lings exhibited lower xylem conductivity and wilted more rapidly under drought,NaCl,and high temperature treat-ments than wild-type plants.Map-based cloning revealed that WI5 encodes an active endo-1,4-β-xylanase from gly-cosyl dehydration family 10,which mainly functions in degrading and reorganizing cell wall xylan.Reverse-transcription polymerase chain reaction andβ-glucuronidase assays revealed that WI5 is highly expressed in stems,especially in internodes undergoing secondary wall as-sembly.RNA sequencing suggested that WI5 plays a unique role in internode growth.Immunohistochemistry and electron microscopy confirmed that wi5 is defective in xylan deposition and secondary cell wall thickening.Lignin deposition and xylan content were markedly re-duced in wi5 compared to the wild-type plants.Our results suggest that WI5 functions in xylem cell wall thickening through its xylanase activity and thereby regulates xylem water transport,the drought stress response,and plant growth in maize. | Xiaojiao Hu Yang Cui Xiaomin Lu Weibin Song Lei Lei Jinjie Zhu Jinsheng Lai Lizhu E Haiming Zhao | 2020 | Journal of Integrative Plant Biology2020，62，10： | 3 |
| 8 | Buckling-controlled two-way shape memory effect in a ring-shaped bilayer显示文摘Shape memory polymers(SMPs)usually have a one-way shape memory effect.In this paper,an easy-operating method to realize a two-way shape memory effect was demonstrated in a ring-shaped bilayer structure where the two layers are SMPs with different thermal transition temperatures.By designing specific thermomechanical processes,the mismatched deformation between the two layers leads to a morphology change of ring-shaped bilayer structures from a smooth ring to a gear-like buckling shape under cooling and a reversible recovery to the smooth shape under heating.Such a morphology change is ascribed to occurrence and recovery of thermoelastic buckling.This method was validated by finite element simulation.We experimentally investigated the influence of pre-strain on buckling,and it was found that both the buckling occurrence and recovery temperature vary with pre-strain.Furthermore,considering a ring-shaped SMP-SMP bilayer structure,finite element analysis was conducted to study the influence of film thickness and modulus ratio of two layers on buckling behavior.The results showed that the critical buckling wavelength was greatly influenced by film thickness and modulus ratio.W e made a theoretical analysis that accorded well with the numerical results. | Hao Li Xiaoyan Liang Weibin Song | 2019 | Acta Mechanica Sinica2019，35，6： | 3 |
| 9 | OS1 functions in the allocation of nutrients between the endosperm and embryo in maize seeds显示文摘Uncovering the genetic basis of seed develop-ment will provide useful tools for improving both crop yield and nutritional value, However, the genetic regulatory networks of maize (Zea mays) seed development remain largely unknown. The maize opaque endosperm and small germ 1 (osl) mutant has opaque endosperm and a small embryo,Here, we cloned OSi1 and show that it encodes a putative transcription factor containing an RWP-RK domain-Transcriptional analysis indicated that OSi1 expression is elevated in early endosperm development, especially in the basal endosperm transfer layer (BETL), conducting zone (CZ), and central starch endospcrm (CSE) cells. RNA sequencing (RNA-Seq) analysis of the osi mutant revealed sharp downregulation of certain genes in specific cell types, including ZmMRP-1 and Megl in BETL cells and a majority of zein-and starch-related genes in CSE cells. Using a haploid induction system, we show that wild-type endosperm could rescue the smaller size of osi embryo, which suggests that nutrients are allocated by tho wild type endosperm. Therefore, our dsta imply that the network regulated by 051 accomplishes a key step in nutrient allocation between endosperm and embryo within maize seeds. Identification of this network will help uncover the mechanisms regulating the nutritional balance between endosperm and embryo. | Weibin Song Jinjie Zhu Haiming Zhao Yingnan Li Jiangtao Liu Xiangbo Zhang Liangliang Huang Jinsheng Lai | 2019 | Journal of Integrative Plant Biology2019，61，6： | 3 |
| 10 | Life History Recorded in the Vagino-cervical Microbiome Along with Multi-omes显示文摘The vagina contains at least a billion microbial cells,dominated by lactobacilli.Here we perform metagenomic shotgun sequencing on cervical and fecal samples from a cohort of 516 Chinese women of reproductive age,as well as cervical,fecal,and salivary samples from a second cohort of 632 women.Factors such as pregnancy history,delivery history,cesarean section,and breastfeeding were all more important than menstrual cycle in shaping the microbiome,and such information would be necessary before trying to interpret differences between vagino-cervical microbiome data.Greater proportion of Bifidobacterium breve was seen with older age at sexual debut.The relative abundance of lactobacilli especially Lactobacillus crispatus was negatively associated with pregnancy history.Potential markers for lack of menstrual regularity,heavy flow,dysmenorrhea,and contraceptives were also identified.Lactobacilli were rare during breastfeeding or post-menopause.Other features such as mood fluctuations and facial speckles could potentially be predicted from the vagino-cervical microbiome.Gut and salivary microbiomes,plasma vitamins,metals,amino acids,and hormones showed associations with the vagino-cervical microbiome.Our results offer an unprecedented glimpse into the microbiota of the female reproductive tract and call for international collaborations to better understand its long-term health impact other than in the settings of infection or pre-term birth. | Zhuye Jie Chen Chen Lilan Hao Fei Li Liju Song Xiaowei Zhang Jie Zhu Liu Tian Xin Tong Kaiye Cai Zhe Zhang Yanmei Ju Xinlei Yu Ying Li Hongcheng Zhou Haorong Lu Xuemei Qiu Qiang Li Yunli Liao Dongsheng Zhou Heng Lian Yong Zuo Xiaomin Chen Weiqiao Rao Yan Ren Yuan Wang Jin Zi Rong Wang Na Liu Jinghua Wu Wei Zhang Xiao Liu Yang Zong Weibin Liu Liang Xiao Yong Hou Xun Xu Huanming Yang Jian Wang Karsten Kristiansen Huijue Jia | 2022 | Genomics, Proteomics & Bioinformatics2022，20，2： | 2 |
| 11 | MP3RNA-seq:Massively parallel 3’end RNA sequencing for high-throughput gene expression profiling and genotyping显示文摘Transcriptome deep sequencing(RNA‐seq)hasbecome a routine method for global geneexpression profiling.However,its application tolarge‐scale experiments remains limited by costand labor constraints.Here we describe amassively parallel 3′end RNA‐seq(MP3RNA‐seq)method that introduces unique samplebarcodes during reverse transcription to permitsample pooling immediately following this initialstep.MP3RNA‐seq allows for handling of hun-dreds of samples in a single experiment,at acost of about$6 per sample for libraryconstruction and sequencing.MP3RNA‐seq iseffective for not only high‐throughput geneexpression profiling,but also genotyping.Todemonstrate its utility,we applied MP3RNA‐seqto 477 double haploid lines of maize.We iden-tified 19,429 genes expressed in at least 50%ofthe lines and 35,836 high‐quality singlenucleotide polymorphisms for genotypinganalysis.Armed with these data,we performedexpression and agronomic trait quantitativetrait locus(QTL)mapping and identified 25,797expression QTLs for 15,335 genes and 21 QTLsfor plant height,ear height,and relative earheight.We conclude that MP3RNA‐seq is highlyreproducible,accurate,and sensitive forhigh‐throughput gene expression profiling andgenotyping,and should be generally applicableto most eukaryotic species. | Jian Chen Xiangbo Zhang Fei Yi Xiang Gao Weibin Song Haiming Zhao Jinsheng Lai | 2021 | Journal of Integrative Plant Biology2021，63，7： | 1 |
| 12 | Genome-wide Nucleosome Occupancy and Organization Modulates the Plasticity of Gene Transcriptional Status in Maize显示文摘Nucleosomes 是染色质的基本单位由 modulating DNA 可接近性在基因规定起关键作用。然而,他们在调整织物特定的基因抄写的角色 ? 糟糕被理解。这里,我们定序 micrococcal 核酸酶产生的玉米射击和内乳的现在的染色体宽的 nucleosome 地图消化了 nucleosomal DNA。在射击和内乳之间的基因 transcriptional 地位的变化被优先的 nucleosome 损失在 transcriptional 开始地点(+1 nucleosome ) 下游、 transcriptional 结束地点(1 nucleosome ) 在上游的第一 nucleosome 从倡导者和移动伴随。内在地编码 DNA 的 nucleosome 组织大部分与基因的能力被联系在不同纸巾之中改变它的 transcriptional 地位。与织物特定的基因相比,组成地表示了显示出的基因更多读了 5 和 3 个弄空 nucleosome 的区域 ? 象一样进一步 ?+1 nucleosome 到 transcriptional 开始地点和到 transcriptional 结束地点的 1 nucleosome。而且,当使用检验在时, nucleosome 组织更高度比与它的表达式水平与基因 transcriptional 地位的粘性被相关 ? vivo 和预言的 nucleosome 数据。另外,织物特定的基因的翻译效率看起来比组成地表示的基因的那些大。一起拿,我们的结果显示那个内在地编码 DNA 的 nucleosome 组织是重要的在它在调整基因表示层次的角色以外,在决定基因 transcriptional 的粘性地位。 | Jian Chen En Li Xiangbo Zhang Xiaomei Dong Lei Lei Weibin Song Haiming Zhao Jinsheng Lai | 2017 | Molecular Plant2017，10，7： | 1 |
| 13 | Molecular mapping and candidate gene analysis of the semi-dominant gene Vestigial glume1 in maize显示文摘The glume is an organ of the maize spikelet and plays important roles in anther and kernel development.Vestigial glume1(Vg1)is a classic mutant associated with ligule and glume development.Here we report the phenotypic characterization,fine mapping,and candidate gene analysis of the Vg1 mutant.Vg1 is a semi-dominant and pleiotropic gene,and also affects plant height,ear height,and tassel length.Vg1 ligule degeneration begins at the first leaf,and the Vg1 tassel and ear can be distinguished from those of wild-type plants when their lengths reach respectively 55 mm and 51 mm.Using a BC3 mapping population of 11,445 plants,we delimited the Vg1 functional site to an interval of 7.4 kb,flanked by the markers InDelLM and CRM6.A putative cyclopropane fatty-acid synthase gene(ZmCPA-FAS1)was hypothesized to underlie the mutant phenotype.We detected a Helitron insertion in the sixth intron of ZmCPAFAS1.Its presence caused abnormal alternative splicing of ZmCPA-FAS1 that conferred new characteristics on the Vg1 mutant.These findings are a basis for further discovery of the molecularmechanism underlying glume development and a potential guide formaize breeding of small-glume varieties,especially sweet corn breeding. | Chaoxian Liu Yuanzeng Zhao Yang Bai Xiaomin Lu Weibin Song Liping Qin Yilin Cai | 2019 | The Crop Journal2019，7，5： | 1 |
| 14 | Clinical practice guideline for body composition assessment based on upper abdominal magnetic resonance images annotated using artificial intelligence显示文摘Introduction Upper abdominal magnetic resonance(MR)imaging is appropriate for body composition analysis.111 Especially for individuals with obesity,it is of great value to quantify the hepatic proton density fat fraction(PDFF)and the amount of abdominal adipose tissue during clinical evaluation and for research on obesity-related risks.Analytical results may be used to determine the optimal choice of surgical procedure and evaluate treatment outcomes.Multiple artificial intelligence(Al)algorithms and systems have been developed for the automated measurement of body composition.The basis of Al development and application is to have uniform standards for clinical data acquisition and management.The uneven quality ofMR images is one ofthe major obstacles to Al system development and analytical results.A standardized process of MR scanning and clinical data management is urgently needed. | Han Lv Mengyi Li Zhenchang Wang Dawei Yang Hui Xu Juan Li Yang Liu Di Cao Yawen Liu Xinru Wu He Jin Peng Zhang Liqin Zhao Rixing Bai Yunlong Yue Bin Li Nengwei Zhang Mingzhu Zou Jinghai Song Weibin Yu Pin Zhang Weijun Tang Qiyuan Yao Liheng Liu Hui Yang Zhenghan Yang Zhongtao Zhang National Clinical Research Center for Digestive Diseases in Beijing Friendship Hospital,Capital Medical University Beijing Quality Control and Improvement Center of Medical Imaging National Health Commission Capacity Building and Continuing Education Center,Chinese College of Metabolic and Bariatric Surgeons Beijing Quality Control and Improvement Center of Metabolic and Bariatric Surgery | 2022 | Chinese Medical Journal2022，，6： | 1 |
| 15 | Survival after radical cystectomy for bladder cancer:Multicenter comparison between minimally invasive and open approaches显示文摘Objective:To investigate oncological outcomes in patients with bladder cancer who underwent minimally invasive radical cystectomy(MIRC)or open radical cystectomy(ORC).Methods:We identified patients with bladder cancer who underwent radical cystectomy(RC)in 13 centers of the Chinese Bladder Cancer Consortium(CBCC).Perioperative outcomes were compared between MIRC and ORC.The influence of surgical approaches on overall survival(OS)and cancer-specific survival(CSS)in the entire study group and subgroups classified according to pathologic stage or lymph node(LN)status was assessed with the log-rank test.Multivariable Cox proportional hazard models were used to evaluate the association among OS,CSS and risk factors of interest.Results:Of 2098 patients who underwent RC,1243 patients underwent MIRC(1087 laparoscopic RC and 156 robotic-assisted RC,respectively),while 855 patients underwent ORC.No significant differences were noted in positive surgical margin rate and 90-day postoperative mortality rate.MIRC was associated with less estimated blood loss,more LN yield,higher rate of neobladder diversion,longer operative time,and longer length of hospital stay.There was no significant difference in OS and CSS according to surgical approaches(pZ0.653,and 0.816,respectively).Subgroup analysis revealed that OS and CSS were not significantly different regardless of the status of extravesical involvement or LN involvement.Multivariable Cox regression analyses showed that the surgical approach was not a significant predictor of OS and CSS.Conclusions:Our study showed that MIRC was comparable to conventional ORC in terms of OS and CSS. | Weibin Xie Junming Bi Qiang Wei Ping Han Dongkui Song Lei Shi Dingwei Ye Yijun Shen Xin Gou Weiyang He Shaogang Wang Zheng Liu Jinhai Fan Kaijie Wu Zhiwen Chen Xiaozhou Zhou Chuize Kong Yang Liu Chunxiao Liu Abai Xu Baiye Jin Guanghou Fu Wei Xue Haige Chen Tiejun Pan Zhong Tu Tianxin Lin Jian Huang | 2020 | Asian Journal of Urology2020，7，3： | 1 |
| 16 | Small extracellular vesicles with nanomorphology memory promote osteogenesis显示文摘Nanotopographical cues endow biomaterials the ability to guide cell adhesion, proliferation, and differentiation. Cellular mechanical memory can maintain the cell status by retaining cellular information obtained from past mechanical microenvironments. Here, we propose a new concept “morphology memory of small extracellular vesicles (sEV)” for bone regeneration. We performed nanotopography on titanium plates through alkali and heat (Ti8) treatment to promote human mesenchymal stem cell (hMSC) differentiation. Next, we extracted the sEVs from the hMSC, which were cultured on the nanotopographical Ti plates for 21 days (Ti8-21-sEV). We demonstrated that Ti8-21-sEV had superior pro-osteogenesis ability in vitro and in vivo. RNA sequencing further confirmed that Ti8-21-sEV promote bone regeneration through osteogenic-related pathways, including the PI3K-AKT signaling pathway, MAPK signaling pathway, focal adhesion, and extracellular matrix-receptor interaction. Finally, we decorated the Ti8-21-sEV on a 3D printed porous polyetheretherketone scaffold. The femoral condyle defect model of rabbits was used to demonstrate that Ti8-21-sEV had the best bone ingrowth. In summary, our study demonstrated that the Ti8-21-sEV have memory function by copying the pro-osteogenesis information from the nanotopography. We expect that our study will encourage the discovery of other sEV with morphology memory for tissue regeneration. | Liang Ma Wencan Ke Zhiwei Liao Xiaobo Feng Jie Lei Kun Wang Bingjin Wang Gaocai Li Rongjin Luo Yunsong Shi Weifeng Zhang Yu Song Weibin Sheng Cao Yang | 2022 | Bioactive Materials2022，7，11： | 1 |
| 17 | HITAC-seq enables high-throughput cost-effective sequencing of plasmids and DNA fragments with identity显示文摘DNA sequencing is vital for many aspects of biological research and diagnostics. Despite the development of second and third generation sequencing technologies, Sanger sequencing has long been the only choice when required to precisely track each sequenced plasmids or DNA fragments. Here, we report a complete set of novel barcoding and assembling system, Highly-parallel Indexed Tagmentation-reads Assembled Consensus sequencing(HITAC-seq), that could massively sequence and track the identities of each individual sequencing sample. With the cost of much less than that of single read of Sanger sequencing,HITAC-seq can generate high-quality contiguous sequences of up to 10 kilobases or longer. The capability of HITAC-seq was confirmed through large-scale sequencing of thousands of plasmid clones and hundreds of amplicon fragments using approximately 100 pg of input DNAs. Due to its long synthetic length, HITACseq was effective in detecting relatively large structural variations, as demonstrated by the identification of a~1.3 kb Copia retrotransposon insertion in the upstream of a likely maize domestication gene. Besides being a practical alternative to traditional Sanger sequencing, HITAC-seq is suitable for many highthroughput sequencing and genotyping applications. | Xiang Gao Weipeng Mo Junpeng Shi Ning Song Pei Liang Jian Chen Yiting Shi Weilong Guo Xinchen Li Xiaohong Yang Beibei Xin Haiming Zhao Weibin Song Jinsheng Lai | 2021 | Journal of Genetics and Genomics2021，48，8： | 0 |
| 18 | Enhancing maize's nitrogen-fixing potential through ZmSBT3, a gene suppressing mucilage secretion∞显示文摘Maize(Zea mays)requires substantial amounts of nitrogen,posing a challenge for its cultivation.Recent work discovered that some ancient Mexican maize landraces harbored diazotrophic bacteria in mucilage secreted by their aerial roots.To see if this trait is retained in modern maize,we conducted a field study of aerial root mucilage(ARM)in 258 inbred lines.We observed that ARM secretion is common in modern maize,but the amount significantly varies,and only a few lines have retained the nitrogen‐fixing traits found in ancient landraces.The mucilage of the high‐ARM inbred line HN5‐724 had high nitrogen‐fixing enzyme activity and abundant diazotrophic bacteria.Our genome‐wide association study identified 17 candidate genes associated with ARM across three environments.Knockouts of one candidate gene,the subtilase family gene ZmSBT3,confirmed that it negatively regulates ARM secretion.Notably,the ZmSBT3 knockout lines had increased biomass and total nitrogen accumulation under nitrogen‐free culture conditions.High ARM was associated with three ZmSBT3 haplotypes that were gradually lost during maize domestication,being retained in only a few modern inbred lines such as HN5‐724.In summary,our results identify ZmSBT3 as a potential tool for enhancing ARM,and thus nitrogen fixation,in maize. | Jingyang Gao Peijiang Feng Jingli Zhang Chaopei Dong Zhao Wang Mingxiang Chen Zhongliang Yu Bowen Zhao Xin Hou Huijuan Wang Zhaokun Wu Razia Sultana Jemim Haidong Yu Doudou Sun Pei Jing Jiafa Chen Weibin Song Xuecai Zhang Zijian Zhou Jianyu Wu | 2023 | Journal of Integrative Plant Biology2023，65，12： | 0 |
| 19 | Object servoing of differential-drive service robots using switched control显示文摘Object servoing is becoming more and more important for service robots. Because of the nonholonomicconstraints of a differential-drive service robot and the possibly changed pose of amovable object, it is challenging to design an object servoing scheme for the differential-driveservice robot such that it can asymptotically park at a predefined relative pose to that of the movableobject. In this paper, a novel object servoing scheme is proposed for the differential-driveservice robots using switched control. Each relative online pose is first estimated by using featuresof the movable object, the estimated pose is an input of an object servoing friendly parkingcontroller. The linear velocity and angular speed are then determined by the proposed controller.Simulation results validate the performance of the proposed object servoing scheme. Due to itslow online computational cost, the proposed scheme can be applied for the real-time tasks ofdifferential-drive service robots to movable objects. | Weibin Jia Wenjie Zhao Zhihuan Song Zhengguo Li | 2023 | Journal of Control and Decision2023，10，3： | 0 |
| 20 | Stabilizing iridium sites via interface and reconstruction regulations for water oxidation in alkaline and acidic media显示文摘Exploring effective iridium(Ir)-based electrocatalysts with stable iridium centers is highly desirable for oxygen evolution reaction(OER).Herein,we regulated the incorporation manner of Ir in Co_(3)O_(4)support to stabilize the Ir sites for effective OER.When anchored on the surface of Co_(3)O_(4)in the form of Ir(OH)_6 species,the created Ir-OH-Co interface leads to a limited stability and poor acidic OER due to Ir leaching.When doped into Co_(3)O_(4)lattice,the analyses of X-ray absorption spectroscopy,in-situ Raman,and OER measurements show that the partially replacement of Co in Co_(3)O_(4)by Ir atoms inclines to cause strong electronic effect and activate lattice oxygen in the presence of Ir-O-Co interface,and simultaneously master the reconstruction effect to mitigate Ir dissolution,realizing the improved OER activity and stability in alkaline and acidic environments.As a result,Ir_(lat)@Co_(3)O_(4)with Ir loading of 3.67 wt%requires 294±4 mV/285±3 mV and 326±2 mV to deliver 10 mA cm^(-2)in alkaline(0.1 M KOH/1.0 M KOH)and acidic(0.5 M H_(2)SO_(4))solution,respectively,with good stability. | Weibin Chen Yanhui Song Lei Li Junjie Guo Zhan Lin | 2024 | Journal of Energy Chemistry2024，89，2： | 0 |
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