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| 1 | Ablation of gut microbiota alleviates obesity-induced hepatic steatosis and glucose intolerance by modulating bile acid metabolism in hamsters显示文摘Since metabolic process differs between humans and mice, studies were performed in hamsters, which are generally considered to be a more appropriate animal model for studies of obesityrelated metabolic disorders. The modulation of gut microbiota, bile acids and the farnesoid X receptor(FXR) axis is correlated with obesity-induced insulin resistance and hepatic steatosis in mice. However,the interactions among the gut microbiota, bile acids and FXR in metabolic disorders remained largely unexplored in hamsters. In the current study, hamsters fed a 60% high-fat diet(HFD) were administeredvehicle or an antibiotic cocktail by gavage twice a week for four weeks. Antibiotic treatment alleviated HFD-induced glucose intolerance, hepatic steatosis and inflammation accompanied with decreased hepatic lipogenesis and elevated thermogenesis in subcutaneous white adipose tissue(sWAT). In the livers of antibiotic-treated hamsters, cytochrome P450 family 7 subfamily B member 1(CYP7 B1) in the alternative bile acid synthesis pathway was upregulated, contributing to a more hydrophilic bile acid profile with increased tauro-β-muricholic acid(TβMCA). The intestinal FXR signaling was suppressed but remained unchanged in the liver. This study is of potential translational significance in determining the role of gut microbiota-mediated bile acid metabolism in modulating diet-induced glucose intolerance and hepatic steatosis in the hamster. | Lulu Sun Yuanyuan Pang Xuemei Wang Qing Wu Huiying Liu Bo Liu George Liu Min Ye Wei Kong Changtao Jiang | 2019 | Acta Pharmaceutica Sinica B2019，9，4： | 29 |
| 2 | Single-cell RNA-seq highlights intra-tumoral heterogeneity and malignant progression in pancreatic ductal adenocarcinoma显示文摘Pancreatic ductal adenocarcinoma (PDAC) is the most common type of pancreatic cancer featured with high intra-tumoral heterogeneity and poor prognosis. To comprehensively delineate the PDAC intra-tumoral heterogeneity and the underlying mechanism for PDAC progression, we employed single-cell RNA-seq (scRNA-seq) to acquire the transcriptomic atlas of 57,530 individual pancreatic cells from primary PDAC tumors and control pancreases, and identified diverse malignant and stromal cell types, including two ductal subtypes with abnormal and malignant gene expression profiles respectively, in PDAC. We found that the heterogenous malignant subtype was composed of several subpopulations with differential proliferative and migratory potentials. Cell trajectory analysis revealed that components of multiple tumor-related pathways and transcription factors (TFs) were differentially expressed along PDAC progression. Furthermore, we found a subset of ductal cells with unique proliferative features were associated with an inactivation state in tumor-infiltrating T cells, providing novel markers for the prediction of antitumor immune response. Together, our findings provide a valuable resource for deciphering the intra-tumoral heterogeneity in PDAC and uncover a connection between tumor intrinsic transcriptional state and T cell activation, suggesting potential biomarkers for anticancer treatment such as targeted therapy and immunotherapy. | Junya Peng Bao-Fa Sun Chuan-Yuan Chen Jia-Yi Zhou Yu-Sheng Chen Hao Chen Lulu Liu Dan Huang Jialin Jiang Guan-Shen Cui Ying Yang Wenze Wang Dan Guo Menghua Dai Junchao Guo Taiping Zhang Quan Liao Yi Liu Yong-Liang Zhao Da-Li Han Yupei Zhao Yun-Gui Yang Wenming Wu | 2019 | Cell Research2019，29，9： | 24 |
| 3 | Neutrophil extracellular traps mediate the crosstalk between glioma progression and the tumor microenvironment via the HMGB1/RAGE/IL-8 axis显示文摘Objective:Neutrophil extracellular traps(NETs)produced by tumor-infiltrating neutrophils(TINs)are associated with poor prognosis in patients with several types of cancer.However,the mechanisms underlying the involvement of NETs in glioma progression remain largely unknown.This study aimed to elucidate the roles of NETs in biological processes that drive the crosstalk between glioma progression and the tumor microenvironment.Methods:Neutrophil infiltration and NETs formation were investigated in glioma tissue through immunohistochemistry,and their relationships with clinicopathological features and outcomes were statistically evaluated.The effects of NETs on glioma cell progression were studied in a co-culture system.In vivo and in vitro experiments validated the reactive oxygen species activity and cytokine production of TINs,as well as the ERK signaling pathway activation and the metastasis of gliomas.Results:Neutrophil infiltration and NETs formation were induced in high-grade glioma compared with low-grade glioma.NETs induced by TINs were determined to be an oncogenic marker of high-grade gliomas and to be involved in cell proliferation and invasion.NETs overproduction promoted glioma cell proliferation,migration,and invasion.Furthermore,HMGB1 was found to bind to RAGE and activate the NF-κB signaling pathway in vitro.In addition,NETs stimulated the NF-κB signaling pathway,thus promoting IL-8 secretion in glioblastoma.Subsequently,IL-8 recruited neutrophils which in turn mediated NETs formation via the PI3 K/AKT/ROS axis in TINs.Conclusions:Our results suggest that NETs produced by TINs mediate the crosstalk between glioma progression and the tumor microenvironment by regulating the HMGB1/RAGE/IL-8 axis.Targeting NETs formation or IL-8 secretion may be an effective approach to inhibit glioma progression. | Caijun Zha Xiangqi Meng Lulu Li Shan Mi Da Qian Ziwei Li Pengfei Wu Shaoshan Hu Shihong Zhao Jinquan Cai Yanhong Liu | 2020 | Cancer Biology & Medicine2020，17，1： | 19 |
| 4 | Finite-time adaptive consensus of a class of multi-agent systems显示文摘Multi-agent systems(MASs) are ubiquitous in natural and artificial systems. This paper aims to establish the finite-time adaptive consensus criterion for a class of MASs with nonlinear dynamics. Traditionally, the finite-time consensus criterion is often established based on the prior information on Lipschitz constants and the eigenvalues of Laplacian matrix. However, it is difficult to acquire the above prior information for most real-world engineering systems. To overcome the above difficulty, this paper develops the finite-time consensus criteria for a class of MASs with nonlinear dynamics via adaptive technique. In detail, we design the finite-time distributed node-based and edge-based adaptive consensus protocols for a class of MASs with fixed and switching topologies. Numerical simulations are also given to validate the proposed finite-time adaptive consensus criterion. | LIU KeXin WU LuLu Lü JinHu ZHU HengHui | 2016 | Science China(Technological Sciences)2016，59，1： | 16 |
| 5 | The Belt and Road:Geographical pattern and regional risks显示文摘Building the Belt and Road is initiatives of China to promote win-win international cooperation in the new era,aiming at green,health,intellect and peace and the joint developme nt with people of the coun tries along the route.Systematic an alysis on envir on mental characteristics,evolutionary tendency and future risks are certainly the scie ntific fun dame ntals of sustainable development for the Belt and Road construction.Applied remote sensing monitoring,statistical analysis,this paper investigates the regional characteristics of climate,topography,soil,hydrology,vegetation cover and terrestrial ecosystems production,as well as socio-economic conditions.Based on the regional characteristics,the Belt and Road is divided into 9 sub-regions:Central and Eastern Europe sub-region with cold and humid climate,Mongolia and Russia sub-region with cold and arid climate,Central and West Asia arid sub-region,Southeast Asia sub-region with warm and humid climate,Pakistan arid sub-region,Bangladesh-India-Myanmar sub-region with warm and humid climate,Eastern China monsoon sub-region,Northwest China arid sub-region and Tibetan Plateau sub-region.Combining modeling simulation with seenario projections,natural disaster assessment methodology is used to assess the risk of extreme events including heat waves,droughts and floods in the coming 30 years(2021-2050).Results show that,on the basis of the regional framework,the western Eurasia would be a warming trend;both sides of Qinghai-Tibet Plateau in high temperature and heat waves risk;Central and Eastern Europe sub-region with cold and humid climate in high drought risk;Bangladesh-lndia-Myanmar sub-region with warm and humid climate as well as Eastern China in high risk of flooding. | WU Shaohong LIU Lulu LIU Yanhua GAO Jiangbo DAI Erfu FENG Aiqing WANG Wentao | 2019 | Journal of Geographical Sciences2019，29，4： | 11 |
| 6 | Molecular Characterization of a Leaf Senescence-Related Transcription Factor BrWRKY75 of Chinese Flowering Cabbage显示文摘WRKY is a plant-specific transcription factor(TF) involved in the regulation of many biological processes; however, its role in leaf senescence of leafy vegetables remains unknown. In the present work, a WRKY TF, termed Br WRKY75 was isolated from Chinese flowering cabbage [Brassica rapa L. ssp. chinensis(L.) Mokino var. utilis Tsen et Lee]. Analysis of deduced amino acid sequence and the phylogenetic tree showed that Br WRKY75 has high homology with WRKY75 from Brassica oleracea and Arabidopsis thaliana, and belongs to the II c sub-group. Sub-cellular localization and transcriptional activity analysis revealed that Br WRKY75 is a nuclear protein with transcriptional repression activity, and was up-regulated during leaf senescence. Electrophoretic mobility shift assay confirmed that Br WRKY75 directly bound to the W-box(TTGAC) cis-element. Collectively,these results provide a basis for further investigation of the transcriptional regulation of Chinese flowering cabbage leaf senescence. | TAN Xiaoli FAN Zhongqi LI Lulu WU Ya KUANG Jianfei LU Wangjin CHEN Jianye | 2016 | Horticultural Plant Journal2016，2，5： | 11 |
| 7 | Mechanics of rib spalling of high coal walls under fully-mechanized mining显示文摘为了解决高煤的肋骨 spalling 的问题,围在充分使机械化(HCWFM ) 矿,我们使用了损坏力学的原则分析煤墙肋骨 spalling。煤墙肋骨 spalling 是的结果表演到某个度,发展的宏表演微裂缝。我们造了一个机械模型模仿损坏到煤墙的前面,执行了损坏参数的理论计算,在煤墙肋骨 spalling 上分析了采矿高度,原来的裂缝,缝接强度,水平应力,煤墙的垂直排水量和另外的参数的效果,它与我们的地大小和数字模拟的结果一致很好。控制煤墙肋骨 spalling 的钥匙是在煤墙中控制裂缝的开发。加速推进工作的脸的速度,改进支持的设定的负担和警卫板的水平力量,加强煤围并且另外的技术措施能有效地在 HCWFM 脸减少煤墙和控制煤墙肋骨 spalling 的损坏的度。 | Yuan Yong Tu Shihao Wu Qi Ma Xiaotao Tu Hongsheng Sun Lulu | 2011 | Mining Science and Technology2011，21，1： | 9 |
| 8 | Correction to:Development of a Novel Reverse Transcription Loop-Mediated Isothermal Amplification Method for Rapid Detection of SARS-CoV-2显示文摘In the original version of this article,the legend to Figure 1 was incorrect.The corrected legend is given below.Fig.1 A Location of the primers in SARS-CoV-2 genome.B Sequence comparison among seven human coronaviruses(SARS-CoV-2,SARS-CoV,MERS-CoV,HCoVOC43,HCoV-HKU-1,HCoV-NL63 and HCoV-229E).C Cross-reactivity test of the novel SARS-CoV-2 RTLAMP assay to other common respiratory viruses.Tested common respiratory viruses include HCoV-HKU-1,HCoV-NL63,HCoV-OC43,HCoV-229E,influenza A,B,and C viruses,parainfluenza viruses type 1-3,enterovirus,respiratory syncytial virus A and B groups,human rhinovirus,human metapneumovirus,adenovirus and bocavirus. | Renfei Lu Xiuming Wu Zhenzhou Wan Yingxue Li Lulu Zuo Jianru Qin Xia Jin Chiyu Zhang | 2020 | Virologica Sinica2020，35，4： | 8 |
| 9 | Development of a Novel Reverse Transcription Loop-Mediated Isothermal Amplification Method for Rapid Detection of SARS-CoV-2显示文摘Dear Editor,Since early December 2019, a large outbreak of pneumonia caused by a novel coronavirus(COVID-19) had emerged in Wuhan, China(Wu et al. 2020 a, b;Zhou et al. 2020;Zhu et al. 2020;Jiang and Shi 2020). Similar to severe acute respiratory syndrome coronavirus(SARS-CoV) and Middle East respiratory syndrome coronavirus(MERS-CoV),the new coronavirus also belongs to Betacoronavirus。 | Renfei Lu Xiuming Wu Zhenzhou Wan Yingxue Li Lulu Zuo Jianru Qin Xia Jin Chiyu Zhang | 2020 | Virologica Sinica2020，35，3： | 7 |
| 10 | Metformin and metabolic diseases: a focus on hepatic aspects显示文摘Metformin 广泛地为类型 2 糖尿病(T2D ) 的处理被用作首要的反糖尿病的药。作为首先指向肝的药, metformin 压制肝的葡萄糖生产(HGP ) ,用作 metformin 由改进 T2D 的多糖症的主要机制。生物化学地, metformin 压制 gluconeogenesis 并且刺激 glycolysis。Metformin 也禁止 glycogenolysis,它是极其贡献提高的 HGP 的一条小径。当在多糖症上产生有益的效果时, metformin 也改进胰岛素抵抗并且与 T2D 在病人改正 dyslipidemia。metformin 的这些有益的效果含有为在管理非酒精的脂肪肝的 metformin 的一个角色疾病。是从人和动物研究由结果支持了, metformin 改进肝的脂肪变性并且压制肝发炎。机械学地,肝的方面上的 metformin 的有益的效果通过腺苷被调停激活 monophosphate 的蛋白质 kinase (AMPK ) 依赖者和 AMPK 独立的小径。另外, metformin 通常是安全的并且可以也与另外的长期的肝疾病有益于病人。 | Juan Zheng Shih-Lung Woo Xiang Hu Rachel Botchlett Lulu Chen Yuqing Huo Chaodong Wu | 2015 | Frontiers of Medicine2015，9，2： | 6 |
| 11 | Direct Ink Writing of Highly Conductive MXene Frames for Tunable Electromagnetic Interference Shielding and Electromagnetic Wave-Induced Thermochromism显示文摘The highly integrated and miniaturized next-generation electronic products call for high-performance electromagnetic interference(EMI)shielding materials to assure the normal operation of their closely assembled components.However,the most current techniques are not adequate for the fabrication of shielding materials with programmable structure and controllable shielding efficiency.Herein,we demonstrate the direct ink writing of robust and highly conductive Ti3C2Tx MXene frames with customizable structures by using MXene/AlOOH inks for tunable EMI shielding and electromagnetic wave-induced thermochromism applications.The as-printed frames are reinforced by immersing in AlCl_(3)/HCl solution to remove the electrically insulating AlOOH nanoparticles,as well as cross-link the MXene sheets and fuse the filament interfaces with aluminum ions.After freeze-drying,the resultant robust and porous MXene frames exhibit tunable EMI shielding efficiencies in the range of 25-80 dB with the highest electrical conductivity of 5323 S m−1.Furthermore,an electromagnetic wave-induced thermochromic MXene pattern is assembled by coating and curing with thermochromic polydimethylsiloxane on a printed MXene pattern,and its color can be changed from blue to red under the high-intensity electromagnetic irradiation.This work demonstrates a direct ink printing of customizable EMI frames and patterns for tuning EMI shielding efficiency and visualizing electromagnetic waves. | Xinyu Wu Tingxiang Tu Yang Dai Pingping Tang Yu Zhang Zhiming Deng Lulu Li Hao-Bin Zhang Zhong-Zhen Yu | 2021 | Nano-Micro Letters2021，13，10： | 3 |
| 12 | Reduced macular inner retinal thickness and microvascular density in the early stage of patients with dysthyroid optic neuropathy显示文摘Background:The goal was to investigate changes of the inner intra-retinal layer thicknesses and retinal capillary density(RCD)around the macula in thyroid-associated ophthalmopathy(TAO)patients with or without dysthyroid optic neuropathy(DON).Methods:Forty-four TAO patients including 23 non-DON and 21 DON patients,and 38 healthy participants were enrolled.Spectral domain optical coherence tomography equipped with Angiovue was used to obtain threedimensional retinal thickness maps and microvascular images of the superficial and deep retinal capillary layers(SRCL and DRCL,respectively)around the macula.Quantitative analyses were performed using a custom automated algorithm.Results:The thicknesses of the nerve fiber layer,ganglion cell layer+inner plexiform layer,and ganglion cell complex(GCC)as well as the RCDs in the SRCL and DRCL in both TAO groups were significantly decreased compared to the controls.In addition,the RCDs in DRCL of the DON group were further decreased compared to the non-DON group.GCC thickness in both TAO groups was positively correlated with the RCDs of the SRCL in the total annular zone and in the temporal,inferior,and nasal sectors.The areas under the receiver operating characteristic curves for the GCC thickness combined with the RCD were generally larger than those of each single indicator.Conclusions:Thinned inner intra-retinal layers and decreased RCDs in the TAO patients without DON revealed that morphological changes might precede visual dysfunction.The composite index of the retinal structure and the microvascular density might be valuable in the diagnosing,monitoring,and intervention for early DON. | Yufei Wu Yunhai Tu Chaoming Wu Lulu Bao Jianhua Wang Fan Lu Meixiao Shen Qi Chen | 2020 | Eye and Vision2020，7，1： | 3 |
| 13 | Tracking SARS-CoV-2 Omicron diverse spike gene mutations identifies multiple inter-variant recombination events显示文摘The current pandemic of COVID-19 is fueled by more infectious emergent Omicron variants.Ongoing concerns of emergent variants include possible recombinants,as genome recombination is an important evolutionary mechanism for the emergence and re-emergence of human viral pathogens.In this study,we identified diverse recombination events between two Omicron major subvariants(BA.1 and BA.2)and other variants of concern(VOCs)and variants of interest(VOIs),suggesting that co-infection and subsequent genome recombination play important roles in the ongoing evolution of SARS-CoV-2.Through scanning high-quality completed Omicron spike gene sequences. | Junxian Ou Wendong Lan Xiaowei Wu Tie Zhao Biyan Duan Peipei Yang Yi Ren Lulu Quan Wei Zhao Donald Seto James Chodosh Zhen Luo Jianguo Wu Qiwei Zhang | 2022 | Signal Transduction and Targeted Therapy2022，7，5： | 3 |
| 14 | Correlation between radiologic features on contrastenhanced CT and pathological tumor grades in pancreatic neuroendocrine neoplasms显示文摘Contrast-enhanced computed tomography(CT)contributes to the increasing detection of pancreatic neuroendocrine neoplasms(PNENs).Nevertheless,its value for differentiating pathological tumor grades is not well recognized.In this report,we have conducted a retrospective study on the relationship between the 2017 World Health Organization(WHO)classification and CT imaging features in 94 patients.Most of the investigated features eventually provided statistically significant indicators for discerning PNENs G3 from PNENs G1/G2,including tumor size,shape,margin,heterogeneity,intratumoral blood vessels,vascular invasion,enhancement pattern in both contrast phases,enhancement degree in both phases,tumor-to-pancreas contrast ratio in both phases,common bile duct dilatation,lymph node metastases,and liver metastases.Ill-defined tumor margin was an independent predictor for PNENs G3 with the highest area under the curve(AUC)of 0.906 in the multivariable logistic regression and receiver operating characteristic curve analysis.The portal enhancement ratio(PER)was shown the highest AUC of 0.855 in terms of quantitative features.Our data suggest that the traditional contrastenhanced CT still plays a vital role in differentiation of tumor grades and heterogeneity analysis prior to treatment. | Wenbin Xu Han Yan Lulu Xu Mingna Li Wentao Gao Kuirong Jiang Junli Wu Yi Miao | 2021 | The Journal of Biomedical Research2021，35，3： | 2 |
| 15 | Mechanics of rib spalling of high coal walls under fully-mechanized mining显示文摘 | Yuan Yong Tu Shihao Wu Qi Ma Xiaotao Tu Hongsheng Sun Lulu | 2011 | Mining Science and Technology (China)2011，，1： | 2 |
| 16 | Winter heat budget in the Huanghai Sea and the effect from Huanghai Warm Current (Yellow Sea Warm Current)显示文摘Four sources of surface heat flux (SHF) and the satellite remote sensing sea surface temperature (SST) data are combined to investigate the heat budget closure of the Huanghai Sea (HS) in winter.It is found that heat loss occurs all over the HS during winter and the area averaged heat content change decreases with a rate of-106 W/m 2.Comparing with the area averaged SHF of-150 W/m 2 from the four SHF data sets,it can be concluded that the SHF plays a dominant role in the HS heat budget during winter.In contrast,the heat advection transported by the Huanghai Warm Current (Yellow Sea Warm Current,HWC) accounted for up to 29% of the HS heat content change.Close correlation,especially in February,between the storm events and the SST increase demonstrates that the HWC behaves strongly as a wind-driven compensation current. | QIAO Lulu WANG Xiaohua WANG Yongzhi WU Dexing BAO Xianwen MU Lin | 2011 | Acta Oceanologica Sinica2011，30，5： | 2 |
| 17 | Hazards of extreme events in China under different global warming targets显示文摘Changes in the frequency and intensity of extreme events affect the risk pattern of risk-bearing bodies and the effectiveness of response measures.Based on future climate scenario data,in this study,we identify global warming periods of 1.5℃ and 2℃ above pre-industrial levels,and the temporal and spatial patterns of temperature and precipitation in China during the same period,propose a methodology for risk assessment and integrated analysis of extreme events,build a database of extreme events and their hazards in China in the context of climate change,and assess the hazards caused by extreme events(i.e.,heatwaves,floods,and droughts)in China under different warming periods.Under the RCP4.5 and RCP8.5 scenarios,global temperatures increase by 1.5℃ and 2℃ above preindustrial levels by 2029 and 2026 and by 2050 and 2040,respectively.The warming would be fast in the north and slow in the south,the warming of the Qinghai-Tibet Plateau would accelerate,and the precipitation would increase in the north and decrease in the south,but the trend of the precipitation reduction in the south would be more significant under the RCP8.5 scenario.For 2℃ of warming,the hazard index of severe heatwaves in the North China Plain,parts of South China,and the western part of Inner Mongolia would double that of 1.5℃ warming.Regional differences in extreme precipitation would increase,especially under the RCP8.5 scenario,the hazard index of severe floods in parts of East China would be about twice that of 1.5℃ warming.Under the joint influence of rising temperature and changing precipitation,the drought situation would be alleviated under the RCP4.5 scenario,but it is intensified under the RCP8.5 scenario.For 2℃ of warming,the hazard index of severe droughts in some areas would reach three times that of 1.5℃ warming.For the integrated hazard of the three types of extreme events,the areas of high and medium hazards would expand,while the areas of low hazard would decrease.For 2℃ of warming,the areas of high and medium hazards would be more than half the total area. | Jiangbo Gao Lulu Liu Shaohong Wu | 2020 | Big Earth Data2020，4，2： | 2 |
| 18 | Solid-state electrolytes for solid-state lithium-sulfur batteries:Comparisons,advances and prospects显示文摘Compared with other secondary batteries,lithium-sulfur batteries(LSBs)have unparalleled advantages such as high energy density,low cost,etc.In liquid LSB systems,it is extremely easy to cause severe‘‘shuttle effecto and safety issues.Hence,the development of solid-state LSBs(SSLSBs)has been attracting much more attention.As the most essential part of the SSLSBs,the solid-state electrolyte(SSE)has received significant attention from researchers.In this review,we concentrate on discussing the core of SSLSBs,which is the SSE.Moreover,we also highlight the differences in the properties of the different SSEs,which are polymer-based electrolytes and ceramic-based electrolytes.In addition,the challenges and advances in different types of SSEs are also compared and described systematically.Furthermore,the prospects for new SSE systems and the design of effective SSE structures to achieve highperformance SSLSBs are also discussed.Thus,this review is expected to give readers a comprehensive and systematic understanding of SSEs for SSLSBs. | Xin Liang Lulu Wang Xiaolong Wu Xuyong Feng Qiujie Wu Yi Sun Hongfa Xiang Jiazhao Wang | 2022 | Journal of Energy Chemistry2022，31，10： | 2 |
| 19 | Effects of Octreotide on Activated Pancreatic Stellate Cell-Induced Pancreas Graft Fibrosis in Rats显示文摘 | Dan Long Jun Lu Lei Luo Yingjia Guo Chengwen Li Wenqiao Wu Juan Shan LuLu Li Shengfu Li YouPing Li Tao Lin Li Feng | 2012 | Journal of Surgical Research2012，，1： | 1 |
| 20 | Cardioprotective effect of Yiqi Huoxue granule through regulation of mitophagy after myocardial infarction in rats显示文摘Objective:To investigate the cardioprotective effect of Yiqi Huoxue granule(YQHXG)in the regulation of autophagy in rats induced with myocardial infarction(MI).Methods:An acute MI animal model was established by ligation of the left anterior descending branch of the coronary artery in Sprague-Dawley rats.Besides,20 rats received sham operation were classified into a control group.The remaining 59 rats were randomly divided into MI model group(n=19),YQHXG group(n=20),and perindopril group(n=20).Relevant indicators on days 7 and 28 were observed in each group.Left ventricular function was determined by echocardiography.The structure and morphology of mitochondria,and the number of autophagic vesicles,were observed by transmission electron microscopy.The mRNA and protein expression levels of LC3,FUNDC1,Beclin-1,and BNIP3 were examined in the tissue of the MI marginal area.Results:Compared with the MI model group,YQHXG showed obvious improvements in cardiac functions.Observing the microscopic morphology of the heart tissue,myocardial tissue damage attenuated,autophagic signs of autophagosomes and autolysosomes reduced,vacuolization in mitochondria mitigated,and mitochondria arranged in order.YQHXG could reduce the degree of tissue lesion after MI and regulate the expression of autophagy-related molecules at different stages.On Day 7,YQHXG significantly downregulated the expression of Fundc1,Becn1,Bnip3 mRNA and reduced the levels of FUNDC1,Beclin-1,BNIP3,and LC3 B proteins expression(all P<.001).On Day 28,YQHXG could upregulate the expression of Becn1,Fundc1 and Bnip3 mRNA and increased the levels of the corresponding proteins expression(all P<.001).Besides,it also increased LC3 B protein expression level(P=.0344).Conclusion:YQHXG regulated the expression of mitochondrial autophagy-related factors in myocardial tissue and mitochondrial autophagic activity at different stages to protect the heart following MI. | Xi Chen Jiangong Wu Shuwen Guo Hanrong Mo Tianwei Zhan Fanghe Li Hui Wang Pengfei Feng Lulu Wei | 2020 | Journal of Traditional Chinese Medical Sciences2020，7，2： | 1 |
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