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3篇 您的检索式:作者名="Takuya Tsubota"
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1Gene expression analysis in the larval silk gland of the eri silkworm Samia ricini显示文摘昆虫为目的一个范围生产丝绸。在鳞翅目,丝绸为茧生产作为材料被利用并且服务保护幼虫免受不利环境条件或食肉动物的伤害。在 Saturniidae 家庭的种类例如,生产茧的一个特别宽的变化有许多小洞的大、金色的有颜色的茧和那些。尽管在国内蚕(Bombyx mori L.) 的丝绸腺的基因表示广泛地被学习了,更加,更少调查集中了于天蚕蛾家庭的成员。这里,我们从 eri 蚕(Samia ricini ) 的丝绸腺建立了表示顺序标签,天蚕蛾种,并且使用这些分析基因表示。尽管我们识别了丝心蛋白在确定的图书馆的重链基因,为另外的主要丝绸蛋白质的基因例如丝心蛋白光链和 fibrohexamerin,是不在的。这发现与这些后者蛋白质缺乏天蚕蛾丝绸的以前的报告一致。最近,一系列象 fibrohexamerin 一样基因在 Bombyx 染色体被识别。我们使用了这个信息进行这里建立的图书馆的详细分析。这分析识别了这些基因的通常认为的相当或相同事物。我们也发现几编码也是在另外的鳞翅目的丝绸的现在的小丝绸蛋白质分子的基因。基因表示模式在 eri 和国内蚕之间被比较,并且保存并且 nonconserved 表示模式为测试基因被识别。如此的微分基因表示可能是在在这些之间的丝绸性质的差别的主要原因之一种类。我们相信我们的学习能作为为丝绸腺基因表示的一个基本目录具有值,它将让步到丝绸进化的进一步的理解。Takuya Tsubota Kimiko Yamamoto Kazuei Mita Hideki Sezutsu 2016Insect Science2016,23,6:2
2Transcriptomic analysis of the bagworm moth silk gland reveals a number of silk genes conserved within Lepidoptera显示文摘Lepidopteran insects produce cocoons with unique properties.The cocoons are made of silk produced in the larval tissue silk gland and our understanding of the silk genes is still very limited.Here,we investigated silk genes in the bagworm moth Eumeta variegata,a species that has recently been found to produce extraordinarily strong and tough silk.Using short-read transcriptomic analysis,we identified a partial sequence of the fibroin heavy chain gene and its product was found to have a C-terminal structure that is conserved within nonsaturniid species.This is in accordance with the presence of fibroin light chain/fibrohexamerin genes and it is suggested that the bagworm moth is producing silk composed of fibroin ternary complex.This indicates that the fibroin structure has been evolutionarily conserved longer than previously thought.Other than fibroins we identified candidates for sericin genes,expressed strongly in the middle region of the silk gland and encoding serine-rich proteins,and other silk genes,that are structurally conserved with other lepidopteran homologues.The bagworm moth is thus considered to be producing conventional lepidopteran type of silk.We further found a number of genes expressed in a specific region of the silk gland and some genes showed conserved expression with Bombyx mori counterparts.This is the first study allowing comprehensive silk gene identification and expression analysis in the lepidopteran Psychidae family and should contribute to the understanding of silk gene evolution as well as to the development of novel types of silk.Takuya Tsubota Taiyo Yoshioka Akiya Jouraku Takao K.Suzuki Naoyuki Yonemura Kenji Yukuhiro Tsunenori Kameda Hideki Sezutsu 2021Insect Science2021,28,4:1
3Genomic and phylogenetic analysis of insect carboxyl/cholinesterase genes显示文摘TSUBOTA TAKUYA S MASARU O 2010Journal of Pesticide Science2010,35,3:1
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