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| 1 | The efficiency of pectinase scouring with agitation to improve cotton fabric wettability 显示文摘 | MICHEH E H L SANDRA K | 2000 | Textile Chem and Colorist & American Dyestuff Reporter2000，，8： | 1 |
| 2 | HP0953-hypothetical virulence factor overexpresion and localization during Helicobacter pylori infection of gastric epithelium显示文摘BACKGROUND The high prevalence and persistence of Helicobacter pylori(H. pylori) infection, as well as the diversity of pathologies related to it, suggest that the virulence factors used by this microorganism are varied. Moreover, as its proteome contains 340hypothetical proteins, it is important to investigate them to completely understand the mechanisms of its virulence and survival. We have previously reported that the hypothetical protein HP0953 is overexpressed during the first hours of adhesion to inert surfaces, under stress conditions, suggesting its role in the environmental survival of this bacterium and perhaps as a virulence factor.AIM To investigate the expression and localization of HP0953 during adhesion to an inert surface and against gastric(AGS) cells.METHODS Expression analysis was performed for HP0953 during H. pylori adhesion. HP0953 expression at 0,3, 12, 24, and 48 h was evaluated and compared using the Kruskal-Wallis equality-of-populations rank test. Recombinant protein was produced and used to obtain polyclonal antibodies for immunolocalization. Immunogold technique was performed on bacterial sections during adherence to inert surfaces and AGS cells, which was analyzed by transmission electron microscopy. HP0953 protein sequence was analyzed to predict the presence of a signal peptide and transmembrane helices, both provided by the ExPASy platform, and using the GLYCOPP platform for glycosylation sites. Different programs, via, I-TASSER, RaptorX, and HHalign-Kbest, were used to perform three-dimensional modeling.RESULTS HP0953 exhibited its maximum expression at 12 h of infection in gastric epithelium cells.Immunogold technique revealed HP0953 localization in the cytoplasm and accumulation in some peripheral areas of the bacterial body, with greater expression when it is close to AGS cells.Bioinformatics analysis revealed the presence of a signal peptide that interacts with the transmembrane region and then allows the release of the protein to the external environment. The programs also showed a similarity with the Tip-alpha protein of H. pylori. Tip-alpha is an exotoxin that penetrates cells and induces tumor necrosis factor alpha production, and HP0953 could have a similar function as posttranslational modification sites were found;modifications in turn require enzymes located in eukaryotic cells. Thus, to be functional, HP0953 may necessarily need to be translocated inside the cell where it can trigger different mechanisms producing cellular damage.CONCLUSION The location of HP0953 around infected cells, the probable posttranslational modifications, and its similarity to an exotoxin suggest that this protein is a virulence factor. | Nancy K Arteaga-Resendiz Gerardo E Rodea Rosa María Ribas-Aparicio Alma L Olivares-Cervantes Juan Arturo Castelán-Vega Joséde Jesús Olivares-Trejo Sandra Mendoza-Elizalde Edgar O López-Villegas Christian Colín Pamela Aguilar-Rodea Alfonso Reyes-López Marcela Salazar García Norma Velázquez-Guadarrama | 2022 | World Journal of Gastroenterology2022，28，29： | 1 |
| 3 | The Application of SHRIMP to Phanerozoic geochronology: a critical appraisal of four zircon standards显示文摘 | Lance P B Sandra L K Ian S Williams | 2003 | Chemical Geology2003，200，： | 1 |
| 4 | TEMORA 1: a new zircon standard for Phanerozoic U-Pb geochronology显示文摘 | Lance P B Sandra L K Charlotte M A | 2003 | Chemical Geology2003，200，： | 1 |
| 5 | IL-27, a Heterodimeric Cytokine Composed of EBI3 and p28 Protein, Induces Proliferation of Naive CD4 + T Cells显示文摘 | Stefan Pflanz Jackie C Timans Jeanne Cheung Rency Rosales Holger Kanzler Jonathan Gilbert Linda Hibbert Tatyana Churakova Marilyn Travis Elena Vaisberg Wendy M Blumenschein Jeanine D Mattson Janet L Wagner Wayne To Sandra Zurawski Terrill K McClanahan Dan | 2002 | Immunity2002，，6： | 1 |
| 6 | Homologous type Ⅱ collagen-induced arthritis in rats显示文摘 | Par L Sandra K Rikard H | 1990 | Arthritis and Rheumatism1990，33，5： | 1 |
| 7 | Determination of chlormequat residues in pears and pear concentrates by Benchtop LC-ESI-MS显示文摘 | ZHAO Y LAZOU K SCHELFAUT M DE REU L SANDRA P | 2000 | Chromatographia2000，51，3： | 1 |
| 8 | Immunogenic Pro- teins of Feline Rhinotraeheitis Virus显示文摘 | Roger K Maes Sandra L Fritsch Lori L | 1984 | Journal of Virology1984，7，： | 1 |
| 9 | TEMORA 1:a new zircon standard for Phanerozoic U-Pb geochronology显示文摘 | Lance P B Sandra L K Charlotte M A | 2003 | Chemical Geology2003，200，： | 1 |
| 10 | TEMORA 1: a new zircon standard for Phanerozoic U-Pb geochronology显示文摘 | Lance P 13 Sandra L K Charlotte M A | 2003 | Chemical Geology2003，200，： | 1 |
| 11 | TEMORA 1: a new zircon standard for Phanerozoic U-Pb geochronology显示文摘 | Lance P B Sandra L K Charlotte M A | 2003 | Chemical Geology2003，200，12： | 1 |
| 12 | Crucial role of stromal cell-derived factor-1α in neointima formation after vascular injury in apolipoprotein e-deficient mice显示文摘 | Andreas S Sandra K Michael L Elisa AL Christian W | 2003 | Circulation2003，108，： | 1 |
| 13 | Work Load, Tension, and Coping: Moderating Effects of Supervisor Support 显示文摘 | Sandra L K | 1988 | Personnel Psychology1988，41，1： | 1 |
| 14 | Molecular map- ping and identification of QTL's associated to oat crown rust par- tial resistance 显示文摘 | Barbosal M M Federizzi L C Sandra C K | 2006 | Euphytiea2006，150，12： | 1 |
| 15 | Chromium is the proximate clastogenic species for lead chromate-induced clastogenicity in human bronchial cells显示文摘 | SANDRA S W AMIE L H MICHAEL E K | 2004 | Mutation Research2004，560，： | 1 |
| 16 | CD73 promotesanthracycline resistance and poor prognosis in triple negativebreast cancer显示文摘 | Sherene L Sandra P Benjamin H K a/ | 2013 | Proc Natl Acad Sci USA2013，110，11： | 1 |
| 17 | Physical abuse during adolescence: Gender differences in the adolescents' perceptions of family functioning and parenting显示文摘 | Suzanne S Victor L Sandra K | 2008 | Child Abuse & Neglect2008，32，1： | 1 |
| 18 | Determination of chlormequat residues in pears and pear concentrates by benchtop LC-ESI-MS显示文摘 | ZHAO Y LAZOU K SCHELFAUT M DEREU L SANDRA P | 2000 | Chromatographia2000，51，9： | 1 |
| 19 | Determination of chlorm equat residues in pears and pear concentrates by bench top LC-ESI-MS显示文摘 | ZHAO Y LAZOU K SCHELFAUT M DEREU L SANDRA P | 2000 | Chromatograph ia2000，51，9： | 1 |
| 20 | TEMORA 1:a newzircon standard for Phanerozoic U-Pb geochronology显示文摘 | Lance P B Sandra L K Charlotte M A | 2003 | ChemicalGeology2003，200，： | 1 |
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