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| 1 | Effects of Danhong Injection(丹红注射液) and Its Main Components on Anticoagulation and Fibrinolysis in Cultured Vein Endothelial Cells显示文摘Objective: To observe the effects of Danhong Injection(丹红注射液) and its main components, including daiclzein and hydroxysafflor yellow A(HSYA), on the anticoagulation, fibrinolysis, anti-apoptosis in hypoxia model of vein endothelial cells(VECs). Methods: VECs were prepared and were put in a hypoxia environment, which consisted of mixed gas of 95% N_2 and 5% CO_2 mixed gas, when reached confluent culture. Five groups used different treatments, including normal control group, hypoxia group, daiclzein group, HSYA group and Danhong Injection group. The VECs were identified by fluorescence double labeling methods. The morphology was observed by a phase contrast microscopy. The effects of Danhong Injection, daiclzein and HSYA on 6 keto prostaglandin F1α(6-keto-PGF1α) level was measured by the method of radioimmunoassay(RIA). Superoxide dismutase(SOD) activity was tested by water soluble tetrazolium salt. The content of malondialdehyde(MDA) was measured by thiobarbituric acid. The activities of tissue-type plasminogen activator(t-PA) and plasminogen activator inhibitor(PAI) were measured by the method of chromogenic substrate. The contents of endothelin(ET) and nitric oxide(NO) were detected by non-equilibrium RIA and enzymelinked immunosorbent assay. Cells apoptosis rate was determined by flow cytometry. Results: Compared with the normal control group, the floating cells number, PAI activity, ET and MDA contents, and cells apoptosis rate in the culture solution of hypoxia group were all significantly increased, whereas the 6-keto-PGF1α and NO contents, and t-PA and SOD activities were decreased significantly(P<0.01). Compared with the hypoxia group, Danhong Injection markedly increased the 6-keto-PGF1α content and SOD activity, regulated PAI and t-PA activities, ET and NO contents, and decreased MDA content and cells apoptosis rate(P<0.05 or P<0.01). Conclusions: Danhong Injection and its main components played an important role in protecting primary VECs from hypoxic damage by regulating the secretion and vasomotor function of VECs. The function of Danhong Injection was most remarkable. | 张宇燕 周惠芬 杨洁红 何昱 陈小强 Katsuyoshi Nishinari 万浩芳 万海同 | 2016 | Chinese Journal of Integrative Medicine2016，22，4： | 14 |
| 2 | 共轭亚油酸水包油型乳液的物理化学稳定性显示文摘共轭亚油酸(conjugated linoleic acid,CLA)是一种具有多重生理功效的不饱和脂肪酸,其氧化稳定性较差,对光、热、氧气很不稳定。以亲水胶体为乳化剂制备CLA的水包油(O/W)型乳液可改善其氧化稳定性,扩大其在食品中的应用。该研究采用改性阿拉伯胶EM2为乳化剂、2种不同黏度的CLA为油相,通过测定乳液颗粒的粒径和粒径分布以及乳液在40℃贮存过程中的过氧化值和茴香胺值,研究了CLA的水包油(O/W)型乳液的物理化学稳定性。结果表明,高质量分数的EM2有利于形成粒径更小且分布均一的乳液颗粒。乳液的氧化稳定性很大程度上依赖于其物理稳定性。对于黏度较小的CLA,在各测试EM2质量分数下,CLA乳液具有较好的物理稳定性,且随着EM2质量分数的增大,乳液氧化稳定性提高。对于黏度较大的CLA,EM2质量分数为5%时,乳液具有较好的物理化学稳定性;增大EM2的质量分数,其稳定性下降。该研究可为今后研究基于乳液技术的功能性因子保护和增效提供参考,有利于CLA在食品行业中的推广应用。 | 徐琼 姚晓琳 王娜娜 田大志 方亚鹏 Glyn O.Phillips Katsuyoshi Nishinari | 2013 | 农业工程学报2013，29，11： | 10 |
| 3 | 钙离子诱导的海藻酸钠/黄原胶凝胶化临界行为研究显示文摘研究了海藻酸钠/黄原胶混合体系的相行为及其对海藻酸钠-钙离子凝胶化临界行为的影响.当海藻酸钠浓度为0.5 wt%时,随着黄原胶的添加,混合体系出现相容、相分离及液晶3种不同的相行为.与纯黄原胶溶液相比,海藻酸钠/黄原胶混合溶液在更低的黄原胶浓度下开始形成液晶,这是由于混合体系中相分离的发生导致了黄原胶有效浓度升高.利用葡萄糖酸内酯(GDL)在线酸化Ca-EDTA,释放钙离子,研究了不同钙离子引入量时(f=[Ca2+]/[COO-])混合体系的黏弹性.Winter-Chambon分析发现临界凝胶点(f gel)随黄原胶浓度的增加而降低.当相分离发生时,临界凝胶点急剧降低,当液晶结构形成后,临界凝胶点呈现上升趋势.通过对比Winter-Chambon方法和临界凝胶点模量松弛法所测得的松弛临界指数(nw和nr),发现黄原胶的添加使海藻酸钠临界凝胶失去结构自相似性.相分离的发生导致临界凝胶结构排列更加致密,而液晶的出现使临界凝胶结构排列相对疏松. | 张帆 毛鹏 孔慧玲 赵萌 方亚鹏 Katsuyoshi Nishinari Glyn O.Phillips | 2013 | 高分子学报2013，23，11： | 7 |
| 4 | Aggregation behaviour and stability of maize germ oil body suspension显示文摘 | Rujira Sukhotu Xiaodi Shi Qi Hu Katsuyoshi Nishinari Yapeng Fang Shuntang Guo | 2014 | Food Chemistry2014，，： | 2 |
| 5 | Gelation behaviour of konjac glucomannan with different molecular weights显示文摘 | Zhang H Yoshimura M Nishinari K et ai | 2001 | Biopolymers2001，59，1： | 1 |
| 6 | Dynamic viscoelastic study on the gelation of 7S globulin from soybeans 显示文摘 | Nagano T Hirotsuka M Mori H Kohyama K Nishinari K | 1992 | Journal of Agricultural and Food Chemistry1992，40，： | 1 |
| 7 | Rheological studies on mixtures of corn starch andkonjac-glucomannan显示文摘 | YOSHIMURA M TAKAYA T NISHINARI K | 1998 | Carbohydrate Polymers1998，35，12： | 1 |
| 8 | Konjac glucomannan显示文摘 | Nishinari K | 2000 | Developments in Food Science2000，，41： | 1 |
| 9 | Konjac glucomannan显示文摘 | NISHINARI K | 2000 | Developments in Food Science2000，41，： | 1 |
| 10 | Single - phase mixe gels of xyloglucan and gellan 显示文摘 | Shinya lkeda Yoko Nitta Bo Sook Kim Theeranan Ternsiripong Rungnaphar Pongsawatmanit Katsuyoshi Nishinari | 2004 | Food Hydrocolloids2004，18，： | 1 |
| 11 | Konjac glucomannan显示文摘 | Nishinari K | 2000 | Developments in Food Science2000，41，25： | 1 |
| 12 | Antimicrobial activities of meropenem against clinically isolated strains in 1997 显示文摘 | Suzuki Y Matsumoto Y Nishinari C | 1999 | Jpn J Antibiot1999，52，12： | 1 |
| 13 | Antimicrobial activities of meropenem against clinically isolated strains in 1997显示文摘 | Suzuki Y Matsumoto Y Nishinari C | 1999 | Jpn J Antibiot1999，52，12： | 1 |
| 14 | Rheological and thermal studies of gel-sol transition in Gellan gum aqueous solutions显示文摘 | Miyoshi E Takaya T Nishinari K | | 0，，2： | 1 |
| 15 | On solid-like rheological behaviors of globular protein solutions显示文摘 | S. Ikeda K. Nishinari | 2001 | Food Hydrocolloids2001，，4： | 1 |
| 16 | Review of the physico -chemical characteristics and properties of konjac mannan显示文摘 | Nishinari K Williams P A Phillips GO | 1992 | Food Hydrocolloids1992，6，： | 1 |
| 17 | Analytical properties of ultradiscrete Burgers equation and role-184 cellular automaton显示文摘 | Nishinari K Takahashi D | 1998 | Phys A1998，31，： | 1 |
| 18 | Totally implantable venous catheters for chemotherapy:experience in 500 patients显示文摘 | Wolosker N Yazbek G Nishinari K | | 0，，： | 1 |
| 19 | Simulation for pedestrian dynamics by real--coded cellular automata (RCA)显示文摘 | YAMAMOTO K KOKUBO S NISHINARI K | 2007 | Physica A: Statistical Mechanics and its ApplicationS2007，379，2： | 1 |
| 20 | Effect of deacetylation rate on gelation kinetics of konjac glucomannan显示文摘 | Gao S Nishinari K | 2004 | Colloids Surf B:Biointerfaces2004，38，34： | 1 |
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