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| 1 | Alkaloid profiling of the traditional Chinese medicine Rhizoma corydalis using high performance liquid chromatography-tandem quadrupole time-of-flight mass spectrometry显示文摘Since alkaloids are the major active constituents of Rhizoma corydalis(RC),a convenient and accurate analytical method is needed for their identification and characterization.Here we report a method to profile the alkaloids in RC based on liquid chromatography-tandem quadrupole time-of-flight mass spectrometry(LC–Q-TOF-MS/MS).A total of 16 alkaloids belonging to four different classes were identified by comparison with authentic standards.The fragmentation pathway of each class of alkaloid was clarified and their differences were elucidated.Furthermore,based on an analysis of fragmentation pathways and alkaloid profiling,a rapid and accurate method for the identification of unknown alkaloids in RC is proposed.The method could also be useful for the quality control of RC. | Mingqian Sun Jianxun Liu Chengren Lin Lan Miao Li Lin | 2014 | Acta Pharmaceutica Sinica B2014，4，3： | 14 |
| 2 | Transcriptome sequencing and characterization of ungerminated and germinated spores of Nosema bombycis显示文摘Nosema bombycis 是一 obligate 利用特殊机制感染 Bombyx mori 的细胞内部的寄生真菌。萌芽,通过哪个 microsporidia 感染主人房间的一个 indispensible 过程,从休眠状态为 microsporidia 被认为是一个关键发展拐弯处点到繁殖状态。因此,阐明在萌芽为寄生虫控制是关键的前后, transcriptome 变化。然而, microsporidia 的萌芽的分子的基础仍然保持未知。调查这个萌芽过程, N 的 transcriptome。bombycis ungerminated 孢子和发芽孢子被定序并且分析。超过 6000 万个高质量的抄本读用 RNA-Seq 技术从这二个组被产生。在汇编以后,分别地, 2756 和 2690 unigenes 被识别并且随后基于已知的蛋白质注解了。在差别的分析表示了基因以后, 66 基因被识别是在这二个组之间的表示的差别(P 0.05 ) 。蛋白质联系磷酸酶的基因是由 RNA-Seq 和 immunoblot 分析决定了首先被识别显著地起来调整,显示那 dephosphorylation 可能潜在地贡献 microsporidia 萌芽。编码涉及 glycometabolism,孢子墙蛋白质和 N 的篦麻毒素 B lectin 的蛋白质的 DEG。bombycis 也被分析。基因和染色体分析的基因本体论和京都百科全书揭示了为一些特定的生物函数和进程负责的基因。在这研究产生的数据集在 N 提供 transcriptome 变化的基本描述。bombycis 在萌芽期间。transcriptome 数据的分析和是与萌芽有关的柔韧的候选人基因的某些功能的基因的鉴定将帮助提供孢子萌芽和侵略的深理解。 | Han Liu Mingqian Li Xinyi He Shunfeng Cai Xiangkang He Xingmeng Lu | 2016 | Acta Biochimica et Biophysica Sinica2016，48，3： | 10 |
| 3 | Internalization of NK cells into tumor cells requires ezrin and leads to programmed cell-in-cell death显示文摘细胞毒素的淋巴细胞是在有缺点的房间的有免疫力的反应和消除的组织的关键播放器。我们以前报导了生来的杀手(NK ) 房间进入目标肿瘤房间,导致在肿瘤房间以内的任何一个目标房间死亡或自我毁灭。然而,它留下了至于在成为主观以后的 NK 房间的命运逃犯并且 heterotypic cell-in-cell 过程最近是否与同型的 cell-in-cell 事件的不同,说出 entosis。这里,我们证明 NK 房间在肿瘤房间以内与 apoptosis 的最终的命运经历一个 cell-in-cell 过程并且表明成为主观过程要求肌动朊细胞骨架管理者, ezrin。设想 NK 房间怎么进入肿瘤房间,我们执行了 NK 房间成为主观的即时双颜色成像分析进肿瘤房间。令人惊讶地,大多数 NK 房间在他们的入口以后承诺规划房间死亡进肿瘤房间,它与在同型的 cell-in-cell 过程观察的 entosis 区别地不同。使内在化的 NK 房间的 apoptotic 房间死亡由 caspase 的激活是明显的 3 并且 DNA 破碎。而且,在成为主观以后的 NK 房间死亡被 caspase 禁止者稀释, Z-VAD-FMK,在肿瘤房间以内作为 NK 房间死亡的模式证实 apoptosis。决定为 NK 房间的入口必要的蛋白质因素进肿瘤房间,我们执行了基于 siRNA 的击倒的分析并且在 NK 房间成为主观发现了 ezrin 的一个关键角色。重要地, ezrin 的调停 PKA 的 phosphorylation 支持 NK 房间成为主观过程。我们的调查结果建议 ezrin 由管理 NK 房间成为主观进肿瘤房间的新奇规章的机制。 | Shan Wang Zhen Guo Peng Xia Tingting Liu Jufang Wang Shan Li Lihua Sun Jianxin Lu Qian Wen Mingqian Zhou Li Ma Xia Ding Xiaoning Wang Xuebiao Yao | 2009 | Cell Research2009，19，12： | 6 |
| 4 | Ricin-B-lectin enhances microsporidia Nosema bombycis infection in BmN cells from silkworm Bombyx mori显示文摘 | Han Liu Mingqian Li Shunfeng Cai Xinyi He Yongqi Shao Xingmeng Lu | 2016 | Acta Biochimica et Biophysica Sinica2016，48，11： | 6 |
| 5 | In vitro testicular organogenesis from human fetal gonads produces fertilization-competent spermatids显示文摘Unlike most organs that mature during the fetal period,the male reproductive system reaches maturity only at puberty with the commencement of spermatogenesis.Robust modelling of human testicular organogenesis in vitro would facilitate research into mechanisms of and factors affecting human spermatogenic failure and male fertility preservation in prepubertal tumor patients.Here,we report successful recapitulation of human testicular organogenesis in vitro from fetal gonadal ridge.Our model displayed the formation of mature seminiferous epithelium and self-renewing spermatogonia.Remarkably,in vitro-derived haploid spermatids have undergone meiotic recombination,and showed increased genetic diversity as indicated by genetic analysis.Moreover,these spermatids were able to fertilize oocytes and support subsequent blastocyst formation.The in vitro testicular organogenesis system described here will play an important role in elucidating the regulation of human testis development and maintaining male fertility in prepubertal cancer patients. | Yan Yuan Laihua Li Qing Cheng Feiyang Diao Qiao Zeng Xiaoyu Yang Yibo Wu Hao Zhang Mingqian Huang Junqing Chen Quan Zhou Yunfei Zhu Rong Hua Jianyu Tian Xin Wang Zuomin Zhou Jie Hao Jinjin Yu Dong Hua Jiayin Liu Xuejiang Guo Qi Zhou Jiahao Sha | 2020 | Cell Research2020，30，3： | 6 |
| 6 | Proteomic analysis of BmN cells (Bombyx morl) in response to infection with Nosema bombycis显示文摘Nosema bombycis (N。bombycis, Nb ) 一 obligate 细胞内部的寄生虫,它能在蚕引起蚕胞子虫病疾病。调查 N 的效果。主人房间上的 bombycis 感染,从没感染 N 的 BmN 房间的 proteomes。在不同感染阶段的 bombycis 与二维的胶化电气泳动和 MALDI-TOF/TOF 集体 spectrometry 被描绘,它鉴别 24 差别与重要紧张差别表示了主人蛋白质(P < 0.05 ) 同时至少指在嘲笑 -- 并且 N。bombycis 感染了房间。尤其是,基因本体论分析证明这些蛋白质涉及许多重要生物反应。在感染阶段期间,涉及精力新陈代谢和氧化应力的蛋白质有起来调整的表示。二蛋白质参予了 ubiquitin 依赖的蛋白质分解代谢的过程有的下面调整的表示。量的即时聚合酶链反应被用来分析这些识别蛋白质的 transcriptional 侧面。一起拿,在为识别蛋白质的生物反应的丰富变化,通常认为的功能,和参予在 N 生产一个主人应答的蛋白质模型。感染 bombycis 的 BmN 房间。这些调查结果在宿主细胞上关于精力缺点寄生虫的效果推进我们的知识。 | Xinyi He Xiangkang He Han Liu Mingqian Li Shunfeng Cai Zhangwuke Fu Xingmeng Lu | 2014 | Acta Biochimica et Biophysica Sinica2014，46，11： | 5 |
| 7 | Nosema bombycis (Microsporidia) suppresses apoptosis in BmN cells (Bombyx mori)显示文摘Nosema bombycis (N。bombycis, Nb ) 一真菌相关并且要求在蚕引起长期的蚕胞子虫病疾病的细胞内部的寄生虫。在感染主人以后,孢子从主人房间获得精力并且幸存几天。在病原体和宿主细胞之间的这共生建议那 N。bombycis 阻止 apoptosis 和宿主细胞的反应的氧种(ROS ) 生产为它的生长和发展创造最佳的环境条件。在这研究,不同方法被用来证明那 N。bombycis 在 BmN 房间压制了 apoptosis。流动 cytometry 分析结果证明孢子在感染以后在 2 和 5 天压制了 BmN 房间的 apoptosis (P < 0.05 ) 。与放线菌素 D (ActD ) 处理相比, BmN 房间的 apoptosis 显然在孢子感染以后被减少(P < 0.01 ) 。在感染以后的 48 个小时, BmN 房间的 ROS 生产在为 6 h 的 ActD 处理以后与那相比是下面调整的。而且, N。bombycis 阻止了 apoptosomes 的形成由下面调整 apaf-1 和细胞色素 C 的表示。另外, N。bombycis 也起来调整 buffy 的表示。西方的污点分析证明孢子减少了在 48 和 98 h 柱子感染的主人细胞色素 C 的水平。因此,我们的结果建议了那 N。bombycis 禁止了主人房间的 mitochondrial apoptotic 小径为它的自己的幸存创造最佳的环境。 | Xinyi He Zhangwuke Fu Mingqian Li Han Liu Shunfeng Cai Nana Man Xingmeng Lu | 2015 | Acta Biochimica et Biophysica Sinica2015，47，9： | 4 |
| 8 | Analysis of the Conservation of T Cell Receptor Alpha and Beta Chain Variable Regions Gene in pp65 Peptide-Specific HLA-A~*0201-Restricted CD8^+ T Cells显示文摘Many viral epitope specific T cell receptors (TCRs) in MHC-matched individuals have been demonstrated to involve conserved amino acid motifs in β chain complementarity-determining region 3 (CDR3). However, it is not sure whether the conserved motifs can also be found in TCR α chain. In previous studies, we developed a modified method to enlarge the percentage of cytomegalovirus (CMV) pp65 peptide-specific CD8+ T cells in PBMC by continuous peptide stimulation in vitro, which provides sufficient number of specific T cells for detection. In this study, we further analyzed the restrictive usage of TCR Vα and Vβ gene families and investigated the CDR3 gene sequence of pp65 peptide-specific CD8+ T cells. Analysis of CDR3 spectratypes suggested a restricted usage of TCR α chain AV8, AV12, AV21, AV31 families and TCR β chain BV3, BV14, BV21, BV23, BV11 families in donor CD8+ T cells stimulated by pp65 peptide. The sequences of these T cells involved similar sequence (TX) G (X) A in CDR3 region of TCR α chain and L (XT) G (X) A in TCR β chain. | Wei Luo Li Ma Qian Wen Mingqian Zhou Xiaoning Wang | 2009 | Cellular & Molecular Immunology2009，6，2： | 3 |
| 9 | A Survey: FPGA-Based Dynamic Scheduling of Hardware Tasks显示文摘To meet the increasing computing needs of various application fields, Field programmable gate array(FPGA) has been widely deployed. In FPGA-based processing, hardware tasks can be better accelerated by allocating appropriate computing resources. Therefore,FPGA-based hardware task scheduling has become one of the mainstream research directions in academia and industry. However, the optimization objectives of existing FPGA-based hardware task scheduling methods are relatively scattered. In this regard, this paper summarizes the research status of hardware task dynamic scheduling from the three essential elements of FPGA processing: time, resources, and power consumption.This paper analyzes, sorts out, categorizes the ideas and implementations of various scheduling methods and analyzes and evaluates optimization effects of various scheduling methods from multiple dimensions. Then, the shortcomings of the existing methods are summarized and some practical applications are introduced. Finally, the research direction of task scheduling based on FPGA is prospected and summarized. | LI Tianyang ZHANG Fan GUO Wei SUN Mingqian CHEN Li | 2021 | Chinese Journal of Electronics2021，30，6： | 2 |
| 10 | Transferrin plays a central role in coagulation balance by interacting with clotting factors显示文摘Coagulation balance is maintained through fine-tuned interactions among clotting factors,whose physiological concentrations vary substantially.In particular,the concentrations of coagulation proteases(pM to nM)are much lower than their natural inactivator antithrombin(AT,〜3μM),suggesting the existence of other coordinators.In the current study,we found that transferrin(norm plasma concentration〜40 μM)interacts with fibrinogen,thrombin,factor Xlla(FXlla),and AT with different affinity to maintain coagulation balance.Normally,transferrin is sequestered by binding with fibrinogen(normal plasma concentration〜10 μM)at a molar ratio of 4:1.In atherosclerosis,abnormally up-regulated transferrin interacts with and potentiates thrombin/FXlla and blocks ATs inactivation effect on coagulation proteases by binding to AT,thus inducing hypercoagulability.In the mouse model,transferrin overexpression aggravated atherosclerosis,whereas transferrin inhibition via shRNA knockdown or treatment with antitransferrin antibody or designed peptides interfering with transferrin-thrombin/FXlla interactions alleviated atherosclerosis.Collectively,these findings identify that transferrin is an important clotting regulator and an adjuster in the maintenance of coagulation balance and modifies the coagulation cascade. | Xiaopeng Tang Zhiye Zhang Mingqian Fang Yajun Han Gan Wang Sheng Wang Min Xue Yaxiong Li Li Zhang Jian Wu Biqing Yang James Mwangi Qiumin Lu Xiaoping Du Ren Lai | 2020 | Cell Research2020，30，2： | 2 |
| 11 | Superoxide dismutase mimetic ability of Mn-doped ZnS QDs显示文摘Superoxide dismutase(SOD) is an important antioxidant enzyme in the body. SOD has special physiological activity and is the primary substance for scavenging free radicals in living organisms.However, the expensive and complex extraction processes, low SOD yield, as well as difficult to store at room temperature have seriously hindered its application pace. Herein, the enzyme mimetic function of Mn-doped ZnS quantum dots(QDs) was discovered. The improved Marklund and McCord method both showed that Mn-doped ZnS QDs possess intrinsic SOD-like activity. The effects of temperature and pH on the mimetic enzyme activity of Mn-doped ZnS QDs have been investigated compared with SOD enzymes.The low cost and easy to synthesize white Mn-doped ZnS QDs with good biocompatibility are expected to be used as a new type of SOD nanozymes in the biology-relevant fields. | Xiangling Ren Mingqian Wang Xing He Zheng Li Jing Zhang Wei Zhang Xudong Chen Hong Ren Xianwei Meng | 2018 | Chinese Chemical Letters2018，29，12： | 2 |
| 12 | Intelligent Passive Detection of Aerial Target in Space-Air-Ground Integrated Networks显示文摘Passive detection of moving target is an important part of intelligent surveillance. Satellite has the potential to play a key role in many applications of space-air-ground integrated networks(SAGIN). In this paper, we propose a novel intelligent passive detection method for aerial target based on reservoir computing networks. Specifically, delayed feedback networks are utilized to refine the direct signals from the satellite in the reference channels. In addition, the satellite direct wave interference in the monitoring channels adopts adaptive interference suppression using the minimum mean square error filter. Furthermore, we employ decoupling echo state networks to predict the clutter interference in the monitoring channels and construct the detection statistics accordingly. Finally, a multilayer perceptron is adopted to detect the echo signal after interference suppression. Extensive simulations is conducted to evaluate the performance of our proposed method. Results show that the detection probability is almost 100% when the signal-to-interference ratio of echo signal is-36 dB, which demonstrates that our proposed method achieves efficient passive detection for aerial targets in typical SAGIN scenarios. | Mingqian Liu Chunheng Liu Ming Li Yunfei Chen Shifei Zheng Nan Zhao | 2022 | China Communications2022，19，1： | 1 |
| 13 | Electro-hydraulic proportional control of twin-cylinder hydraulic elevators显示文摘 | Li Ke Mannan M A Xu Mingqian | 2001 | Control Engineering Practice2001，9，4： | 1 |
| 14 | Electro- hydraulic proportional control of twin-cyllnder hy- draulic elevator 显示文摘 | Li Ke Mannan M A Xu Mingqian | 2001 | Control Engineering Practice2001，，9： | 1 |
| 15 | Fractional Frequency Offset Estimation for OFDM Systems in Non-Cooperative Communication显示文摘The traditional fractional frequency offset(FFO) estimation schemes for orthogonal frequency division multiplexing(OFDM) in non-cooperative communication have the problems of susceptible performance with the frequency offset values and the number of OFDM symbols,a novel fractional frequency offset blind estimation scheme based on EKF for OFDM systems is conceived.The nonlinear function of the frequency offset is calculated by employing the correlation.And then the frequency offset is estimated by means of the iterative algorithm of EKF.The finally fractional frequency offset is estimated by adopting repeated the above process.Simulation results demonstrate that the proposed scheme is robust to the frequency offset values without any requirements of a prior knowledge. | Mingqian Liu Jian Chen Bingbing Li Junfang Li | 2016 | China Communications2016，13，9： | 1 |
| 16 | Electro-hydraulic proportional control of twin-cylinder hydraulic elevators显示文摘 | Li Ke Mannan M A Xu Mingqian etc | 2001 | Control Engineering Practice (S0967-0661)2001，9，： | 1 |
| 17 | Cell Cluste- ring and Resource Allocation Scheme for CoMP SU-MI- MO 显示文摘 | LIU Mingqian LI Bingbing TONG Zhao | 2013 | Wireless Personal Communications2013，，69： | 1 |
| 18 | A Modified Peptide Stimulation Method for Efficient Amplification of Cytomegalovirus (CMV)-Specific CTLs显示文摘CMV-specific immunity is essential for control of human cytomegalovirus (HCMV) infection. Stem cell trans-plantation is used widely in the management of a range of diseases of the hemopoietic system. Patients are immunosuppressed profoundly in the early posttransplant period, and reactivation of cytomegalovirus (CMV) remains a significant cause of morbidity and mortality. Adoptive transfer of CMV-specific CD8+ T cell clones has been shown to reduce the rate of viral reactivation; however, the ex vivo production of cells for adoptive transfer is labor intensive and expensive. We report here a modified peptide stimulation method using CMV-specific epitope peptides to stimulate PBMCs for generation of CMV-specific CTLs. This method permits efficient amplification of CMV-specific CTLs and provides a large number of cells for FACS analysis from a single blood sample. Significantly, it achieves high frequencies of tetramer staining of CD8+ T cells allowing the data of different individuals to be easily compared and sequentially evaluated. Thus, this approach expands and selects HLA-restricted CMV-pp65-reactive T-cell lines of high specificity for potential adoptive immunotherapy. | Guangping Ruan Li Ma Qian Wen Wei Luo Mingqian Zhou Xiaoning Wang | 2008 | Cellular & Molecular Immunology2008，5，3： | 1 |
| 19 | Responses of dissolved organic carbon and dissolved nitrogen in surface water and soil to CO2enrichment in paddy field显示文摘 | Guo Jia Zhang Mingqian Zhang Li | 2011 | Agriculture Ecosystems and Environment2011，140，： | 1 |
| 20 | Responses of dissolved organic carbon and dissolved nitrogen in surface water and soil to CO 2 enrichment in paddy field显示文摘 | Jia Guo Mingqian Zhang Li Zhang Aixing Deng Xinmin Bian Jianguo Zhu Weijian Zhang | 2010 | Agriculture Ecosystems and Environment2010，，1： | 1 |
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