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| 1 | Pilose antler aqueous extract promotes the proliferation and osteogenic differentiation of bone marrow mesenchymal stem cells by stimulating the BMP-2/Smad1, 5/Runx2 signaling pathway显示文摘Peptides from Pilose antler aqueous extract(PAAE) have been shown to stimulate the proliferation and differentiation of bone marrow mesenchymal stem cells(BMSCs). However, the underlying molecular mechanisms are not well understood. Here, PAAE was isolated and purified to explore the molecular mechanisms underlying PAAE’s effects on BMSCs as well as its osteoprotective effects in ovariectomized rats. Our results showed that PAAE promoted proliferation and differentiation of BMSCs to become osteoblasts by enhancing ALP activity and increasing extracellular matrix mineralization. The trabecular microarchitecture of ovariectomized rats was also found to be protected by PAAE. Quantitative reverse transcription-polymerase chain reaction(Quantitative RT-PCR) results suggest that PAAE also increased the expression of osteogenic markers including, alkaline phosphatase(ALP), runt-related transcription factor 2(Runx2), osteocalcin(OCN), bone morphogenetic protein-2(BMP-2), and collagen I(COL-I). Immunoblotting results indicated that PAAE upregulated the levels of BMP-2 and Runx2 and was associated with Smad1/5 phosphorylation. PAAE A at the concentration of 200μg·mL^-1 showed the strongest effect on proliferation and osteogenic differentiation of BMSCs after 48 h. Using matrix-assisted laser desorption/ionization time of flight mass spectrometry(MALDI-TOF MS), we identified the molecular weight of PAAE A and found that it is less than 3000 Da and showed several significant peaks. In conclusion, PAAE activates the BMP-2/Smad1, 5/Runx2 pathway to induce osteoblastic differentiation and mineralization in BMSCs and can inhibit OVX-induced bone loss. These mechanisms are likely responsible for its therapeutic effect on postmenopausal osteoporosis. | REN Cong GONG Wei LI Feng XIE Ming | 2019 | Chinese Journal of Natural Medicines2019，17，10： | 25 |
| 2 | TANDEM and GMAW Twin Wire Welding of Q690 Steel Used in Hydraulic Support显示文摘Compared with using semi-automatic gas shielded arc welding, using automatic TANDEM twin wire welding and twin wire gas metal arc welding (GMAW) to weld Q690 steel, a low-alloy high-strength structural steel used in the hydraulic support in the fully-mechanized mining face, the welding speed, deposition rate, production environment and welding quality can be obviously improved. Compared with GMAW twin wire welding, a refined microstructure in the weld and heat-affected zone (HAZ), narrow HAZ and improved joint strength were achieved with TANDEM on Q690. Also, due to the push-pull pulsed way in TANDEM welding, the droplet transfer, distribution on heat flow and interaction between two arcs were completely different from those in GMAW twin wire system. The heat input of TANDEM is only about 76.6% of GMAW, and correspondingly, the welding speed and welding seam can be obviously improved. The complete oscillation caused by TANDEM pulsed current occurred in the welding pool, which refined the grains in the microstructure. The results show that TANDEM twin wire welding is very suitable in the welding of Q690 used in the hydraulic support. | FANG Chen-fu MENG Xiao-hui HU Qing-xian WANG Feng-jiang REN He WANG Hai-song GUO Yu MAO Ming | 2012 | Journal of Iron and Steel Research(International)2012，19，5： | 22 |
| 3 | 亚太地区胃食管反流病的处理共识:更新版显示文摘背景与目的:自从2004年亚太地区胃食管反流病(GERD)共识发表以来,更多关于GERD流行病学和处理的文献资料相继出现。有必要对这些资料进行循证综述,对共识作出更新。方法:由多学科专家组应用德尔菲(Delphi)法制定共识条文,提呈相关资料,并对证据质量、推荐力度和共识水平进行分级。结果:亚洲GERD发生率日益增加。其危险因素包括老年、男性、种族、家族史、社会经济地位高、体重指数增加和吸烟。对于有典型症状而无报警症状的患者,对质子泵抑制剂(PPI)试验有症状应答具有诊断意义。如PPI试验失败,停止治疗后pH监测结果阴性可排除GERD。窄带成像、胶囊内镜检查和无线pH监测的作用尚未明确。亚洲诊断策略的制定须考虑到并存的胃癌和消化性溃疡。减轻体质量和抬高床头可改善反流症状。PPIs是最有效的内科治疗手段。对于非糜烂性反流病(NERD)患者,按需治疗较为适宜。有慢性咳嗽、喉炎和典型GERD症状的患者在排除非GERD病因后,应予PPI每天两次治疗。如有经验丰富的外科医师,GERD患者可行胃底折叠术。除临床试验外,GERD不应采用内镜治疗。结论:新的诊断方法和内镜治疗的作用有待进一步研究阐明。亚洲GERD诊断策略的制定须考虑到并存的胃癌和消化性溃疡。PPIs仍为治疗的基石。 | Kwong Ming Fock Nicholas J Talley Ronnie Fass Khean Lee Goh Peter Katelaris Richard Hunt Michio Hongo Tiing Leong Ang Gerald Holtmann Sanjay Nandurkar San Ren Lin Benjamin CY Wong Francis KL Chan Abdul Aziz Rani Young-Tae Bak Jose Sollano Khek Yu Ho Sathoporn Manatsathit 钱本余 | 2008 | 胃肠病学2008，13，7： | 21 |
| 4 | Expression of insulin-like growth factor Ⅱ and its receptor in liver cells of chronic liver diseases显示文摘ExpressionofinsulinlikegrowthfactorⅡanditsreceptorinlivercelsofchronicliverdiseasesYANGDongHua1,XIUChong1,YANGBo1,GUJianR... | YANG Dong Hua 1, XIU Chong 1, YANG Bo 1, GU Jian Ren 2, QIAN Lian Fang 2 and QU Shu Ming 2 | 1997 | World Journal of Gastroenterology1997，3，2： | 21 |
| 5 | Sequencing of PCR amplified HBV DNA pre-c and c regions in 2.2.15 cells and antiviral action by targeted antisense oligonucleotide directed against sequence显示文摘INTRODUCTIONManystudieshaveshownthatantisenseoligonucleotides(ASONs)canefficientlyinhibitHBVDNAreplicationandexpresioninvitro... | ZHOUG Sen 1, WEN Shou Ming 2, ZHANG Ding Feng 3, WANG Quan Li 4, WANG Seng Qi 4 and REN Hong 3 | 1998 | World Journal of Gastroenterology1998，4，5： | 16 |
| 6 | Paleogene Tectonic Evolution Controls on Sequence Stratigraphic Patterns in the Central Part of Deepwater Area of Qiongdongnan Basin, Northern South China Sea显示文摘In active rift basins, tectonism is extremely important for sequence stratigraphic patterns, affecting both the sequence architecture and internal makeup. Sequence stratigraphic framework of a Paleogene rift succession in Qiongdongnan Basin, northern South China Sea, was built using seismic profiles, complemented by well logs and cores. One first-order and three second-order sequences were identified on the basis of basin-scale unconformities, and seven third-order sequences are defined by unconformities along the basin margins and correlative conformities within the central basin. Through unconformity analysis and backstripping procedure, the Paleogene synrift tectonic evolution of deepwater area of Qiongdongnan Basin was proved to be episodic, which can be divided into rifting stage-I, rifting stage-II and rifting stage-III. Episodic rifting resulted in the formation of various types of structural slope break belts, which controlled different architectures and internal makeup of sequences. This study enhances the understanding of the control of tectonic evolution on sequence stratigraphic patterns and establishes relevant patterns in a typical rift basin, and further proposes the favorable sandstone reservoirs developing in different sequence stratigraphic patterns, which will be pretty helpful for subtle pool exploration in deepwater area of petroliferous basins. | Guangzeng Song Hua Wang Huajun Gan Zhipeng Sun Xiaolong Liu Meng Xu Jinfeng Ren Ming Sun Di Sun | 2014 | Journal of Earth Science2014，25，2： | 16 |
| 7 | Non-invasive ventilation improves hemorheology status in hypoxemic patients with acute myocardial infarction after PCI显示文摘血氧不足有时在经皮的冠的干预(一种总线标准) 以后与尖锐心肌的梗塞(AMI ) 在病人发生在紧急情况房间里,甚至在那些与常规高流动的氧吸入的管理。现在的学习是在与 AMI 和 hypoxemia.MethodsThis 在病人改进血氧内容和 hemorheology 评估非侵略的通风(NIV ) 的有效性的 of 未来的学习与 AMI 注册了 50 个连续合格病人(72.3 ??? 折?? | Xi-Fu WANG Ming YE Dong YAN Hui-Min ZHANG Ping JIA Xue-Jun REN Yu-Jie ZENG | 2017 | Journal of Geriatric Cardiology2017，14，4： | 15 |
| 8 | Copper-chelating therapeutic effect in Wilson disease with different clinical phenotypes and polymorphisms of ATP7B gene显示文摘CopperchelatingtherapeuticefectinWilsondiseasewithdiferentclinicalphenotypesandpolymorphismsofATP7BgeneRENMingShan1,HUWen?.. | REN MingShan1, HU WenBin1, ZHANG Zhi1, JU ShuangWu1, FAN YuXin2, WANG GongQiang1 and YANG RenMin1Keywords Wilson disease/therapy copper chelating agents ATP7B gene mutations | 1998 | World Journal of Gastroenterology1998，4，4： | 15 |
| 9 | Polysaccharides from Tricholoma matsutake and Lentinus edodes enhance 5-fluorouracil-mediated H22 cell growth inhibition显示文摘OBJECTIVE: Few studies have investigated the effects produced by combinations of polysaccharides and chemotherapeutic drugs in cancer treatment.We hypothesized that a combinationo fpolysaccha rides(COP) from Lentinus edodes and Tricholoma matsutake would improve the efficacy of 5-fluorouracil(5-FU)-mediated inhibition of H22 cell growth.METHODS: Mice were injected H22 cells and then treated with either 5-FU, polysaccharides from Tricholoma matsutake(PTM), polysaccharides from Lentinus edodes(PL), PTM+PL, 5-FU+PTM, 5-FU+PL, or 5-FU+COP. The tumor weight and volume,and splenic CD4 + and CD8 + T cell frequencies,were determined. Additionally, splenic natural killer(NK) cell and cytotoxic Tlymphocyte (CTL) activities were assessed and the serum levels of tumor necrosis factor-α(TNF-α),Interleukin-2(IL-2),and Interferon-γ (IFN-γ) were measured. 5-FU, PL, PTM, PTM+PL, 5-FU+PL, and 5-FU+PTM groups, mice treated with 5-FU+COP showed:(a)significantly reduced tumor weight and volume(P<0.05);(b) significantly higher serum levels ofTNF-α,IL-2, and IFN-γ(P<0.05);(c) significantly increased CD4+andCD8+Tcell frequencies in the spleen(P<0.05); and(d) significantly increased splenic NK cell and CTL activities(P<0.05). The tumor weight and volume in mice treated with 5-FU+PL or 5-FU+PTM were significantly reduced compared with mice treated with 5-FU alone(P<0.05). Serum levels of TNF-α, IL-2, and IFN-γ, frequencies of CD4 + and CD8+T cells in the spleen, and splenic NK and CTL activities were also significantly increased in mice treated with 5-FU+PL or 5-FU+PTM compared with mice treated with 5-FU alone (P<0.05).CONCLUSION: Polysaccharides from Lentinus edodes and Tricholoma matsutake could enhance the efficacy of 5-FU-mediated H22 cell growth inhibition. | Ming Ren Lingyan Ye Xiaoshi Hao Zhixing Ren Shuping Ren Kun Xu Juan Li | 2014 | Journal of Traditional Chinese Medicine2014，34，3： | 15 |
| 10 | Biomass-derived nitrogen-doped hierarchical porous carbon as efficient sulfur host for lithium–sulfur batteries显示文摘Lithium-sulfur(Li-S) battery is a potential energy storage technology with high energy density and low cost. However, the gap between theoretical expectation and practical performance limits its wide implementation. Herein, we report a nitrogen-doped porous carbon derived from biomass pomelo peel as sulfur host material for Li-S batteries. The hierarchical porous architecture and the polar surface introduced by N-doping render a favorable combination of physical and chemical sulfur confinements as well as an expedite electron/ion transfer, thus contributing to a facilitated and stabilized sulfur electrochemistry. As a result, the corresponding sulfur composite electrodes exhibit an ultrahigh initial capacity of 1534.6 mAh g^-1, high coulombic efficiency over 98% upon 300 cycles, and decent rate capability up to 2 C. This work provides an economical and effective strategy for the fabrication of advanced carbonaceous sulfur host material as well as the significant improvement of Li-S battery performance. | Qinghuiqiang Xiao Gaoran Li Minjie Li Ruiping Liu Haibo Li Pengfei Ren Yue Dong Ming Feng Zhongwei Chen | 2020 | Journal of Energy Chemistry2020，29，5： | 15 |
| 11 | The ATP-binding Cassette Transporter OsABCG15 is Required for Anther Development and Pollen Fertility in Rice显示文摘植物男性繁殖开发是复杂生物进程,但是内在的机制不好理解。这里,我们描绘了米饭(Oryza sativa L.) 男无菌的异种。基于基于地图的克隆和顺序分析,我们在腺苷 triphosphate (ATP ) 识别了 1,459-bp 删除有约束力的盒子(ABC ) transporter 基因, OsABCG15,引起的反常花药和男绝育。因此,我们说出这变异的 osabcg15。表示分析证明 OsABCG15 从舞台 8 在发展花药明确地被表示(成熟分裂 II 阶段) 上演 10 (迟了的小孢子阶段) 。二基因 CYP704B2 和 WDA1,为花药表皮和花粉 exine 的建立在 very-long-chain 的生合成包含了丰满的酸,是在 osabcg15 异种的 downregulated,建议那 OsABCG15 可以从 tapetal 房间在与 sporopollenin 生合成或 sporopollenin 转移有关的过程玩关键功能到花药小腔。一致地,组织学的分析证明 osabcg15 异种在 postmeiotic tapetum 退化开发了明显的反常,导致年轻小孢子的快速的 degredation。结果建议 OsABCG15 在 exine 形成和花粉开发起一个关键作用,类似于在 Arabidopsis 的 AtABCG26 的相应基因。这个工作是有用的在米饭花药开发理解规章的网络。 | Bai-Xiao Niu Fu-Rong He Ming He Ding Ren Le-Tian Chen Yao-Guang Liu | 2013 | Journal of Integrative Plant Biology2013，55，8： | 14 |
| 12 | Micro RNA-1290 promotes esophageal squamous cell carcinoma cell proliferation and metastasis显示文摘AIM:To investigate the biological role of mi R-1290 in esophageal squamous cell carcinoma(ESCC) progression and invasion and the underlying mechanism.METHODS:Quantitative real-time polymerase chain reaction(q RT-PCR) was performed to evaluate mi R-1290 expression in ESCC tissue samples.The roles of mi R-1290 in cell proliferation,migration and invasion were identified using mi R-1290 mimic-transfected cells.In addition,the regulatory effect of mi R-1290 on suppressor of cancer cell invasion(SCAI) was evaluated using q RT-PCR,Western blot analysis and a dual luciferase reporter assay.RESULTS:mi R-1290 was significantly upregulated in ESCC tissue samples compared with normal adjacent tissues(9.213 ± 1.150 vs 1.000 ± 0.0),(P < 0.01).Upregulation of mi R-1290 was associated with tumor differentiation(P = 0.021),N classification(P = 0.006) and tumor-node-metastasis stage(P = 0.021) in ESCC patients.Moreover,ectopic mi R-1290 expression potently promoted ESCC cell growth(P < 0.01),migration(P < 0.01) and invasion(P < 0.01) in vitro.mi R-1290 overexpression in ESCC cell lines decreased SCAI expression at the translational level and reduced SCAI-driven luciferase-reporter activity(P < 0.01).CONCLUSION:Our findings suggested that mi R-1290 may play an oncogenic role in cellular processes of ESCC. | Ming Li Xiao-Yan He Zhi-Mei Zhang Shuo Li Li-Hua Ren Ri-Sheng Cao Ya-Dong Feng Yin-Lin Ji Ye Zhao Rui-Hua Shi | 2015 | World Journal of Gastroenterology2015，21，11： | 13 |
| 13 | Vagus Nerve Stimulation for Pediatric and Adult Patients with Pharmaco-resistant Epilepsy显示文摘 | Fan-Gang Meng Fu-Min Jia Xiao-Hui Ren Yan Ge Kai-Liang Wang Yan-Shan Ma Ming Ge Kai Zhang Wen-Han HU Xin Zhang Wei Hu Jian-Guo Zhang | 2015 | Chinese Medical Journal2015，，19： | 13 |
| 14 | Uptake of HIV Self-testing among Men Who have Sex with Men in Beijing, China: a Cross-sectional Study显示文摘Objective To examine HIV self-testing uptake and its determinates among men who have sex with men(MSM) in Beijing, China. Methods A cross-sectional online survey was conducted in Beijing, China in 2016. Participants were users of a popular Chinese gay networking application and had an unknown or negative HIV status. Univariate and multivariate logistic regression analyses were conducted to examine factors associated with HIV self-testing based on adjusted odds ratio(AOR) and 95% confidence interval(CI). Results Among the 5,996 MSM included in the study, 2,383(39.7%) reported to have used HIV self-testing kits. Willingness to use an HIV self-test kit in the future was expressed by 92% of the participants. High monthly income(AOR = 1.49; CI = 1.10-2.02; P = 0.010), large number of male sex partners(≥ 2: AOR = 1.24; CI = 1.09-1.43; P = 0.002), sexual activity with commercial male sex partners(≥ 2: AOR = 1.94; CI = 1.34-2.82; P = 0.001), long-term drug use(AOR = 1.42; CI = 1.23-1.62; P < 0.001), and long-term HIV voluntary counseling and testing(VCT) attendance(AOR = 3.62; CI = 3.11-4.22; P < 0.001) were all associated with increased odds of HIV self-testing uptake. Conclusion The nearly 40% rate of HIV self-testing uptake among MSM in our sample was high. In addition, an over 90% willingness to use kits in the future was encouraging. HIV self-testing could be an important solution to help China achieve the global target of having 90% of all people living with HIV diagnosed by 2020. | REN Xian Long WU Zun You MI Guo Dong MCGOOGAN Jennifer ROU Ke Ming ZHAO Yan | 2017 | Biomedical and Environmental Sciences2017，30，6： | 13 |
| 15 | Prostate cancer in Asia:A collaborative report显示文摘The incidence of prostate cancer(PCa)within Asian population used to be much lower than in the Western population;however,in recent years the incidence and mortality rate of PCa in some Asian countries have grown rapidly.This collaborative report summarized the latest epidemiology information,risk factors,and racial differences in PCa diagnosis,current status and new trends in surgery management and novel agents for castration-resistant prostate cancer.We believe such information would be helpful in clinical decision making for urologists and oncologists,health-care ministries and medical researchers. | Rui Chen Shancheng Ren 无 Ming Kwong Yiu Ng Chi Fai Wai Sam Cheng Lap Hong Ian Seiji Naito Tadashi Matsuda Elijah Kehinde Ali Kural Jason Yichun Chiu Rainy Umbas Qiang Wei Xiaolei Shi Liqun Zhou Jian Huang Yiran Huang Liping Xie Lulin Ma Changjun Yin Danfeng Xu Kexin Xu Zhangqun Ye Chunxiao Liu Dingwei Ye Xin Gao Qiang Fu Jianquan Hou Jianlin Yuan Dalin He Tiejun Pan Qiang Ding Fengshuo Jin Benkang Shi Gongxian Wang Xiuheng Liu Dongwen Wang Zhoujun Shen Xiangbo Kong Wanhai Xu Yaoliang Deng Haibo Xia Alexa NCohen Xu Gao Chuanliang Xu Yinghao Sun | 2014 | Asian Journal of Urology2014，1，1： | 13 |
| 16 | Enhanced invasion in vitro and the distribution patterns in vivo of CD133+ glioma stem cells显示文摘背景最近的研究建议了癌症干细胞基于他们的电阻引起肿瘤复发到放射疗法和 chemotherapy.Although glioblastoma 房间的高度侵略的性质也在当前的治疗的失败被含有,癌症干细胞是否涉及学习试图估计在 Sprague-Dawley ( SD )老鼠大脑肿瘤从 C6 glioma 房间线和 GSC 的分发模式导出的 glioma 干细胞( GSC )的侵略能力的 invasiveness.This 。 | YU Sheng-ping YANG Xue-jun ZHANG Bin MING Hao-lang CHEN Cong REN Bing-cheng LIU Zhi-feng LIU Bin | 2011 | Chinese Medical Journal2011，，17： | 12 |
| 17 | Large-field high-resolution two-photon digital scanned light-sheet microscopy显示文摘 | Weijian Zong Jia Zhao Xuanyang Chen Yuan Lin Huixia Ren Yunfeng Zhang Ming Fan Zhuan Zhou Heping Cheng Yujie Sun Liangyi Chen | 2015 | Cell Research2015，25，2： | 12 |
| 18 | Q-switched and mode-locked Er-doped fiber laser using PtSe2 as a saturable absorber显示文摘We report a passively Q-switched and mode-locked erbium-doped fiber laser(EDFL) based on PtSe_2, a new two-dimensional material, as a saturable absorber(SA). Self-started Q-switching at 1560 nm in the EDFL was achieved at a threshold pump power of 65 mW, and at the maximum pump power of 450 m W, the maximum single Q-switched pulse energy is 143.2 n J. Due to the polarization-dependent characteristics of the PtSe_2-based SA, the laser can be switched from the Q-switched state to the mode-locked state by adjusting the polarization state. A mode-locked pulse train with a repetition rate of 23.3 MHz and a pulse width of 1.02 ps can be generated when the pump power increases to about 80 m W, and the stable mode-locked state is maintained until the pump power reaches its maximum 450 m W. The maximum single mode-locked pulse energy is0.53 nJ. This is the first time to our knowledge that successful generation of stable Q-switched and mode-locked pulses in an Er-doped fiber laser has been obtained by using PtSe_2 as a saturable absorber. | KANG ZHANG MING FENG YANGYANG REN FANG LIu XINGSHUO CHEN JIE YANG XIAO-QING VAN FENG SONG JIANGUO TIAN | 2018 | Photonics Research2018，6，9： | 10 |
| 19 | ABNORMAL FLOWER AND GRAIN 1 encodes OsMADS6 and determines palea identity and affects rice grain yield and quality显示文摘The palea and lemma are floral organ structures unique to grasses;these structures form the hull and directly affect grain size and quality. However, the molecular mechanisms controlling the development of the hull are not well understood. In this study, we characterized the rice(Oryza sativa) abnormal flower and grain1(afg1) mutant, a new allele of OsMADS6. Similar to previously characterized osmads6 alleles, in the afg1 floret, the palea lost its marginal region and acquired the lemma identity. However, in contrast to other osmads6 alleles, the afg1 mutant showed altered grain size and grain quality, with decreased total starch and amylose contents, and increased protein and soluble sugar contents. The analysis of transcriptional activity suggested that AFG1 is a transcriptional activator and may affect grain size by regulating the expression levels of several genes related to cell expansion and proliferation in the afg1 mutant. These results revealed that AFG1 plays an important role in determining palea identity and affecting grain yield and quality in rice. | Xiaoqi Yu Saisai Xia Qiankun Xu Yuanjiang Cui Ming Gong Dali Zeng Qiang Zhang Lan Shen Guiai Jiao Zhenyu Gao Jiang Hu Guangheng Zhang Li Zhu Longbiao Guo Deyong Ren Qian Qian | 2020 | Science China(Life Sciences)2020，63，2： | 10 |
| 20 | Ganoderma lucidum extract ameliorates MPTP-induced parkinsonism and protects dopaminergic neurons from oxidative stress via regulating mitochondrial function, autophagy, and apoptosis显示文摘Neuroprotection targeting mitochondrial dysfunction has been proposed as an important therapeutic strategy for Parkinson's disease. Ganoderma lucidum (GL) has emerged as a novel age nt that protects neurons from oxidative stress. However, the detailed mechanisms underlying GL-induced neuroprotection have not been documented. In this study, we investigated the neuroprotective effects of GL extract (GLE) and the underlying mechanisms in the classic MPTP(1 -methyl-4-phenyl-1,2,3,6- tetrahydropyridine)-induced mouse model of PD. Mice were injected with MPTP to in duce parkins on ism. The n the mice were administered GLE (400 mg kg^-1 d^-1,ig) for 4 weeks. We observed that GLE administration significantly improved locomotor performanee and increased tyrosine hydroxylase expression in the substantia nigra pars compact (SNpc) of MPTP-treated mice. In in vitro study, treatment of neuroblastoma neuro-2a cells with 1 -methyl-4-phenyIpyridinium (MPP^+, 1 mmol/L) caused mitochondrial membrane potential collapse, radical oxygen species accumulation, and ATP depletion. Application of GLE (800 pg/mL) protected n euroblastoma neu ro-2a cells agai nst MPP in suit. Application of GLE also improved mitochondrial movement dysfunction in cultured primary mesencephalic neurons. In addition, GLE counteracted the decline in NIX (also called BNIP3L) expression and increase in the LC3-II/LC3-I ratio evoked by MPP I Moreover, GLE reactivated MPP^+-inhibited AMPK, mTOR, and ULK1. Similarly, GLE was sufficient to counteract MPP1 -induced inhibition of PINK1 and Parkin expression. GLE suppressed MPP^+-induced cytochrome C release and activation of caspase-3 and caspase-9. In summary, our results provide evidence that GLE ameliorates parkinsonism pathology via regulating mitochondrial function, autophagy, and apoptosis, which may involve the activation of both the AMPK/mTOR and PINK1/Parkin signaling pathway. | Zhi-li Ren Chao-dong Wang Tao Wang Hui Ding Ming Zhou Nan Yang Yan-yong Liu Piu Chan | 2019 | Acta Pharmacologica Sinica2019，40，4： | 10 |
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