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| 1 | In vitro assay of cytoskeleton nanomechanics as a tool for screening potential anticancer effects of natural plant extract, tubeimoside I on human hepatoma (HepG2) cells显示文摘Cytoskeleton nanomechanics characterizes cancer cell's physical behaviors such as how it spread and invade. For anticancer drug, cytoskeleton nanomechanics may be a target to inhibit invasiveness and metastasis of cancer cells. Therefore, in vitro assay of cytoskeleton nanomechanics may be used to evaluate the effects of potential anticancer drug on various cancer types. Here, we investigated the effects of tubeimoside I (TBMS I) on human hepatoma (HepG2) cells by using optical magnetic twisting cytometry, a well-established technique for measuring nanomechanics of the Factin cytoskeleton. TBMS I is a natural compound extracted from a traditional Chinese herbal medicine, and is reported with antitumor effect. In this study, we demonstrated that the cytoskeleton stiffness (G) of HepG2 cells was affected by TBMS I. G′ exhibited a typical power law with respect to the loading frequency (f), i.e. G^f . The magnitude of G′ and the value of exponent (α) of the HepG2 cells decreased consistently with the increase of concentration for TBMS I exposure. In addition, the HepG2 cells responded to TBMS I much faster than the normal liver (L-02) cells. Such alteration of cytoskeleton nanomechanics induced by TBMS I was reported for the first time, which was further corroborated by assays of Factin cytoskeleton structure and cell migration. Taken together, these results suggest that in vitro assay of cytoskeleton nanomechanics may have a great potential as an additional tool in screening of anticancer drug candidates. | ZHAO HongBo WANG YaShu JIANG XueMei SHI XiaoHao ZHONG HongZhe WANG YaJie CHEN Jun DENG LinHong | 2013 | Chinese Science Bulletin2013，58，21： | 2 |
| 2 | Experimental research on oxygen-enriched gasification of straw in an entrained-flow gasifier 显示文摘 | Yu Haimiao Li Zeshen Yang Xianyu Jiang Linhong Zhang Ze Chen Dezhen | 2013 | Journal of Renewable and Sustainable Energy2013，5，05： | 1 |
| 3 | Soluble ADAM33 determines mechanical behaviors of airway smooth muscle cells显示文摘Introduction A disintegrin and metalloproteinase 33(ADAM33)has been identified as a susceptibility gene for asthma[1],In particular,ADAM33 mRNA is highly expressed in airway smooth muscle cells(ASMCs)and the level of soluble ADAM33(sADAM33)is known to inversely correlate with lung function such as bronchial hyperresponsiveness(BHR)[2]that is ultimately determined by the mechanical behaviors of ASMCs.However,few have studied how sADAM33would affect ASMC mechanics.Here,we manipulated the expression of SADAM33 in | Yiyuan Duan Jiaoyue Long Feng Lin Jun Chen Rong Xu Xuemei Jiang Linhong Deng | 2013 | 医用生物力学2013，28，S1： | 1 |
| 4 | The Application of Virtual Reality Technology in Constructing Virtual Machining Workshop显示文摘 | Linhong Xu Zhengfeng Jiang Hong Lu | 2008 | Lecture Notes in Computer Science2008，，： | 1 |
| 5 | Geographic Variation in Cardiovascular Health as Analyzed from the China Cardiovascular Health Index Study——31 PLADs,China,2017-2021显示文摘Summary What is already known about this topic?The prevalence of cardiovascular disease(CVD)in China is high,while effective prevention and proper management is lacking.No available indicators were found before 2016 that could comprehensively evaluate different aspects of CVD prevention and treatment. | Fan Mao Yingying Jiang Jing Liu Yan Zhang Yong Jiang Linhong Wang Dong Zhao Yong Huo Junbo Ge Maigeng Zhou | 2022 | China CDC weekly2022，4，13： | 1 |
| 6 | Morphine Re-arranges Chromatin Spatial Architecture of Primate Cortical Neurons显示文摘The expression of linear DNA sequence is precisely regulated by the three-dimensional(3D)architecture of chromatin.Morphine-induced aberrant gene networks of neurons have been extensively investigated;however,how morphine impacts the 3D genomic architecture of neurons is still unknown.Here,we applied digestion-ligation-only high-throughput chromosome conformation capture(DLO Hi-C)technology to investigate the effects of morphine on the 3D chromatin architecture of primate cortical neurons.After receiving continuous morphine administration for 90 days on rhesus monkeys,we discovered that morphine re-arranged chromosome territories,with a total of 391 segmented compartments being switched.Morphine altered over half of the detected topologically associated domains(TADs),most of which exhibited a variety of shifts,followed by separating and fusing types.Analysis of the looping events at kilobase-scale resolution revealed that morphine increased not only the number but also the length of differential loops.Moreover,all identified differentially expressed genes from the RNA sequencing data were mapped to the specific TAD boundaries or differential loops,and were further validated for changed expression.Collectively,an altered 3D genomic architecture of cortical neurons may regulate the gene networks associated with morphine effects.Our finding provides critical hubs connecting chromosome spatial organization and gene networks associated with the morphine effects in humans. | Liang Wang Xiaojie Wang Chunqi Liu Wei Xu Weihong Kuang Qian Bu Hongchun Li Ying Zhao Linhong Jiang Yaxing Chen Feng Qin Shu Li Qinfan Wei Xiaocong Liu Bin Liu Yuanyuan Chen Yanping Dai Hongbo Wang Jingwei Tian Gang Cao Yinglan Zhao Xiaobo Cen | 2023 | Genomics, Proteomics & Bioinformatics2023，21，3： | 0 |
| 7 | Simvastatin Blocks Reinstatement of Cocaine-induced Conditioned Place Preference in Male Mice with Brain Lipidome Remodeling显示文摘Drug-associated reward memories are conducive to intense craving and often trigger relapse.Simvastatin has been shown to regulate lipids that are involved in memory formation but its influence on other cognitive processes is elusive.Here,we used a mass spectrometry-based lipidomic method to evaluate the impact of simvastatin on the mouse brain in a cocaine-induced reinstatement paradigm.We found that simvastatin blocked the reinstatement of cocaine-induced conditioned place preference(CPP)without affecting CPP acquisition.Specifically,only simvastatin administered during extinction prevented cocaine-primed reinstatement.Global lipidome analysis showed that the nucleus accumbens was the region with the greatest degree of change caused by simvastatin.The metabolism of fatty-acids,phospholipids,and triacylglycerol was profoundly affected.Simvastatin reversed most of the effects on phospholipids induced by cocaine.The correlation matrix showed that cocaine and simvastatin significantly reshaped the lipid metabolic pathways in specific brain regions.Furthermore,simvastatin almost reversed all changes in the fatty acyl profile and unsaturation caused by cocaine.In summary,pre-extinction treatment with simvastatin facilitates cocaine extinction and prevents cocaine relapse with brain lipidome remodeling. | Wei Xu Yuman He Jiamei Zhang Hongchun Li Xuemei Wan Menglu Li Yonghai Wang Rui Xu Haoluo Zhang Yanping Dai Haxiaoyu Liu Linhong Jiang Ying Zhao Xiaobo Cen | 2021 | Neuroscience Bulletin2021，37，12： | 0 |
| 8 | REAL-t1, an Effective Approach for t1-Noise Suppression in NMR Spectroscopy Based on Resampling Algorithm显示文摘Summaryof main observation and conclusionIn multidimensional(nD)NMR spectroscopy,t1noise usually appears as ridges along indirect dimen-sions,and affects observation of weak signals.The main source of t1noise is instrumental instability,which causes random variation of FID amplitude during data acquisitions and introduces random noise-like peaks into spectrum after Fourier transformation.A number of efforts have been devoted,in order to develop new method or to improve existing approaches for suppressing t1noise.Herein,we propose a novel t1noise suppression method based on resampling algorithm for data processing,shortenedas REAL-t1.The method was verified using simulated 2D spectra,and NOESY spectra of sucrose and protein GB1,showing that the spectral quality was improved in all cases.The performance of REAL-t1was also compared with another recently pro-posed method,which showed that these two methods provided similar performance while REAL-t1cost much shorter experimental time. | Linhong Song Jiannan Wang Xuncheng Su Xu Zhang Conggang Li Xin Zhou Daiwen Yang Bin Jiang Maili Liu | 2020 | Chinese Journal of Chemistry2020，38，1： | 0 |
| 9 | OPTICALLY TRACKING THE MOTION OF MICROBEADS TO STUDY PHYSICAL BEHAVIORS OF THE LIVING CELL IN RESPONSE TO TRANSIENT STRETCH OR COMPRESSION显示文摘Optical magnetic twisting cytometry and traction force microscopy are two advanced cell mechanics research tools that employ optical methods to track the motion of microbeads that are either bound to the surface or embedded in the substrate underneath the cell.The former measures rheological properties of the cell such as cell stiffness,and the latter measures cell traction force dynamics.Here we describe the principles of these two cell mechanics research tools and an example of using them to study physical behaviors of the living cell in response to transient stretch or compression.We demonstrate that,when subjected to a stretchunstretch manipulation,both the stiffness and traction force of adherent cells promptly reduced,and then gradually recover up to the level prior to the stretch.Immunofluorescent staining and Western blotting results indicate that the actin cytoskeleton of the cells underwent a corresponding disruption and reassembly process almost in step with the changes of cell mechanics.Interestingly,when subjected to compression,the cells did not show such particular behaviors.Taken together,we conclude that adherent cells are very sensitive to the transient stretch but not transient compression,and the stretch-induced cell response is due to the dynamics of actin polymerization. | LINHONG DENG XUEMEI JIANG CHENG CHEN AIJING SONG FENG LIN | 2011 | Journal of Innovative Optical Health Sciences2011，4，2： | 0 |
| 10 | Phenotypic plasticity and taxonomy of Cladophora gracilis(Griffiths)Kützing(Cladophorales,Chlorophyta)in the western Yellow Sea with the implication of its DNA barcode显示文摘Due to extensive morphological plasticity,the taxonomy of Cladophora species remains unclear.As one of the widely distributed species,C.gracilis was reported to hold many varieties,which make it difficult to identify the species.This study explored the morphology diversity of nine C.gracilis samples collected along the coast of western Yellow Sea.Some samples showed extremely varied characteristics,by which one cannot classify them correctly.Hence,18S rDNA and nuclear ribosomal DNA internal transcribed spacer regions(ITS)sequences were employed to delimit species.For 18S rDNA,sequence similarity ranged from 99.6%to 100%.For ITS region,the similarity ranged from 98.7%to 100%.Molecular data strongly suggested that the morphologically heterogeneous samples were actually the same species.Characteristics comparison of the samples revealed that the taxonomy criteria including branching pattern and density,thallus color,height and texture varied widely,influenced by environmental conditions and age of alga.Besides,cell dimensions,as the relatively stable criterion,also exhibited intraspecific variance.Successful application of 18S rDNA and ITS sequences indicated that molecular method can be a powerful assistant as DNA barcodes to traditional morphology taxonomy. | Bingxin Huang Linhong Teng Jingjing Jiang Lanping Ding | 2020 | Acta Oceanologica Sinica2020，39，10： | 0 |
| 11 | The effect of inhibitor peptide for ADAM8 in attenuating ovalbumin-induced murine asthma显示文摘Targeted peptides have been identified as showing great promise for treatment of various diseases including asthma.Asthma is considered of difficuIt-to-treat due to its unclear etiology,thus usually requiring life-long treatment.Current strategies for asthma therapy are hampered with undesirable side effects,poor targeting and failure in modulating airway hyperresponsiveness,leading to pressing need of developing more targeted and effective therapeutic sites for asthma.Recently,a disintegrin and metalloproteinase 8(ADAM8)have been shown to over- | Jun Chen Xuemei Jiang Yiyuan Duan Jiaoyue Long Feng Lin Rong Xu Xiaojuan Zhang Linhong Deng | 2013 | 医用生物力学2013，28，S1： | 0 |
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