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| 1 | Positioning accuracy assessment for the 4GEO/5IGSO/2MEO constellation of COMPASS显示文摘Determined to become a new member of the well-established GNSS family,COMPASS(or BeiDou-2) is developing its capabilities to provide high accuracy positioning services.Two positioning modes are investigated in this study to assess the positioning accuracy of COMPASS' 4GEO/5IGSO/2MEO constellation.Precise Point Positioning(PPP) for geodetic users and real-time positioning for common navigation users are utilized.To evaluate PPP accuracy,coordinate time series repeatability and discrepancies with GPS' precise positioning are computed.Experiments show that COMPASS PPP repeatability for the east,north and up components of a receiver within China's Mainland is better than 2 cm,2 cm and 5 cm,respectively.Apparent systematic offsets of several centimeters exist between COMPASS precise positioning and GPS precise positioning,indicating errors remaining in the treatments of COMPASS measurement and dynamic models and reference frame differences existing between two systems.For common positioning users,COMPASS provides both open and authorized services with rapid differential corrections and integrity information available to authorized users.Our assessment shows that in open service positioning accuracy of dual-frequency and single-frequency users is about 5 m and 6 m(RMS),respectively,which may be improved to about 3 m and 4 m(RMS) with the addition of differential corrections.Less accurate Signal In Space User Ranging Error(SIS URE) and Geometric Dilution of Precision(GDOP) contribute to the relatively inferior accuracy of COMPASS as compared to GPS.Since the deployment of the remaining 1 GEO and 2 MEO is not able to significantly improve GDOP,the performance gap could only be overcome either by the use of differential corrections or improvement of the SIS URE,or both. | ZHOU ShanShi CAO YueLing ZHOU JianHua HU XiaoGong TANG ChengPan LIU Li GUO Rui HE Feng CHEN JunPing WU Bin | 2012 | Science China(Physics,Mechanics & Astronomy)2012，55，12： | 31 |
| 2 | Mutation profile and its correlation with clinicopathology in Chinese hepatocellular carcinoma patients显示文摘Background:Hepatocellular carcinoma(HCC)is one of the most common causes of cancer worldwide.Although many studies have focused on oncogene characteristics,the genomic landscape of Chinese HCC patients has not been fully clarified.Methods:A total of 165 HCC patients,including 146 males and 19 females,were enrolled.The median age was 55 years(range,27-78 years).Corresponding clinical and pathological information was collected for further analysis.A total of 168 tumor tissues from these patients were selected for next-generation sequencing(NGS)-based 450 panel gene sequencing.Genomic alterations including single nucleotide variations(SNV),short and long insertions and deletions(InDels),copy number variations,and gene rearrangements were analyzed.Tumor mutational burden(TMB)was measured by an algorithm developed in-house.The top quartile of HCC was classified as TMB high.Results:A total of 1,004 genomic alterations were detected from 258 genes in 168 HCC tissues.TMB values were identified in 160 HCC specimens,with a median TMB of 5.4 Muts/Mb(range,0-28.4 Muts/Mb)and a 75%TMB of 7.7 Muts/Mb.The most commonly mutated genes were TP53,TERT,CTNNB1,AXIN1,RB1,TSC2,CCND1,ARID1A,and FGF19.SNV was the most common mutation type and C:G>T:A and guanine transformation were the most common SNVs.Compared to wild-type patients,the proportion of Edmondson grade III-IV and microvascular invasion was significantly higher in TP53 mutated patients(P<0.05).The proportion of tumors invading the hepatic capsule was significantly higher in TERT mutated patients(P<0.05).The proportion of Edmondson grade I-II,alpha fetoprotein(AFP)<25μmg/L,and those without a history of hepatitis B was significantly higher in CTNNB1 mutated patients(P<0.05).CTNNB1 mutations were associated with TMB high in HCC patients(P<0.05).Based on correlation analysis,the mutation of TP53 was independently correlated with microvascular invasion(P=0.002,OR=3.096)and Edmondson grade III-IV(P=0.008,OR=2.613).The mutation of TERT was independently correlated with tumor invasion of the liver capsule(P=0.001,OR=3.030),and the mutation of CTNNB1 was independently correlated with AFP(<25μmg/L)(P=0.009,OR=3.414).Conclusions:The most frequently mutated genes of HCC patients in China were TP53,TERT,and CTNNB1,which mainly lead to the occurrence and development of HCC by regulating the P53 pathway,Wnt pathway,and telomere repair pathway.There were more patients with microvascular invasion and Edmondson III-IV grade in TP53 mutated patients and more patients with hepatic capsule invasion in TERT mutated patients,while in CTNNB1 mutated patients,there were more patients with Edmondson I-II grade,AFP<25μmg/L,and a non-hepatitis B background.Also,the TMB values were significantly higher in CTNNB1 mutated patients than in wild type patients. | Shuo Wang Huasheng Shi Tao Liu Manjiang Li Sanshun Zhou Xuan Qiu Zusen Wang Weiyu Hu Weidong Guo Xiaoqian Chen Honglin Guo Xiaoliang Shi Junping Shi Yunjin Zang Jingyu Cao Liqun Wu | 2021 | Hepatobiliary Surgery and Nutrition2021，10，2： | 8 |
| 3 | Intrastriatal glial cell line-derived neurotrophic factors for protecting dopaminergic neurons in the substantia nigra of mice with Parkinson disease显示文摘BACKGROUND:Substantia nigra is deep in position and limited in range,the glial cell line-derived neurotrophic factor(GDNF)injection directly into substantia nigra has relatively greater damages with higher difficulty.GDNF injection into striatum,the target area of dopaminergic neuron,may protect the dopaminergic neurons in the compact part of substantia nigra through retrograde transport.OBJECTIVE:To investigate the protective effect of intrastriatal GDNF on dopaminergic neurons in the substantia nigra of mice with Parkinson disease(PD),and analyze the action pathway.DESIGN:A controlled observation.SETTING:Neurobiological Laboratory of Xuzhou Medical College.MATERIALS:Twenty-four male Kunming mice of 7-8 weeks old were used.GDNF,1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine(MPTP)were purchased from Sigma Company(USA);LEICAQWin image processing and analytical system.METHODS:The experiments were carried out in the Neurobiological Laboratory of Xuzhou Medical College from September 2005 to October 2006.The PD models were established in adult KunMing mice by intraperitoneal injection of MPTP.The model mice were were randomly divided into four groups with 6 mice in each group:GDNF 4-day group,phosphate buffer solution(PSB)4-day group,GDNF 6-day group and PSB 6-day group.Mice in the GDNF 4 and 6-day groups were administrated with 1 μL GDNF solution(20 μg/L,dispensed with 0.01 mol/L PBS)injected into right striatum at 4 and 6 days after model establishment.Mice in the PSB 4 and 6-day groups were administrated with 0.01 mol/L PBS of the same volume to the same injection at corresponding time points.② On the 12th day after model establishment,the midbrain tissue section of each mice was divided into 3 areas from rostral to caudal sides.The positive neurons of tyroxine hydroxylase(TH)and calcium binding protein(CB)with obvious nucleolus and clear outline were randomly selected for the measurement,and the number of positive neurons in unit area was counted.MAIN OUTCOME MEASURES:Number of positive neurons of TH and CB in midbrain substantia nigra of mice in each group.RESULTS:All the 24 mice were involved in the analysis of results.The numbers of TH+ and CB+ neurons in the GDNF 4-day group(54.33±6.92,46.33±5.54)were obviously more than those in the PBS 4-day group(27.67±5.01,21.50±5.96,P < 0.01).The numbers of TH+ and CB+ neurons in the GDNF 6-day group(75.67±5.39,69.67±8.69)were obviously more than those in the PBS 6-day group(27.17±4.50,21.33±5.72,P < 0.01)and those in the GDNF 4-day group(P < 0.01).CONCLUSION:Intrastriatal GDNF can protect dopaminergic neurons in substantia nigra of PD mice,and it may be related to the increase of CB expression. | Chenghua Xiao Yanqiang Wang Hongmei Liu Hongjun Wang Junping Cao Dianshuai Gao | 2007 | Neural Regeneration Research2007，2，4： | 4 |
| 4 | Investigation on Infection of PCV2 in Taizhou Areas of Jiangsu Province显示文摘In order to investigate infection and harm extent of swine porcine circovirus 2( PCV2) in pigs in Taizhou of Jiangsu,and to control the prevalence of PCV2 in Taizhou areas. A total of 116 PCV2 clinical samples came from six different cities( districts) from 2011 to 2012 were tested in etiologic by the method of PCR. The test results showed that the total positive rate of PCV2 clinical samples was 53. 45%,and PCV2 existed in all the six places. The positive rate of the scale pig farms was 48. 33%,the positive rate of free-range farmers was 58. 93%. Thus,PCV2 was prevalent in pigs in Taizhou areas,and which should be paid more attention to. | Cao Junping Guo Guangfu Wu Yantao Lu Guiping Zhu Aiping | 2015 | Animal Husbandry and Feed Science2015，7，4： | 2 |
| 5 | Physiological and anatomical changes in two rapeseed (Brassica napus L.) genotypes under drought stress conditions显示文摘Understanding the mechanisms of drought resistance in crop species is crucial for the selection and breeding of tolerant rapeseed(Brassica napus L.)varieties.The present study aimed to assess the physiological and anatomical responses of two rapeseed genotypes,P287(drought-tolerant)and T88(drought-sensitive)under three intensities of drought stress.All physiological and anatomical parameters related to drought acclimation were significantly altered in both genotypes under stress conditions.At the fourth-leaf stage,the relative water content,chlorophyll content,protein content,malondialdehyde content,and the activities of peroxidase and catalase in P287 were significantly higher than those in T88,particularly under severe drought conditions.After rehydration,all physiological indexes recovered rapidly,especially in P287.In addition,under drought stress,compared with T88,P287 had thicker palisade tissue,thinner spongy tissue,higher ratio of chloroplast length to chloroplast width,higher stomatal density and stomatal closure rate.Overall,the interaction between physiological and anatomical features improved the drought tolerance of P287 under drought stress conditions. | Jiacheng Zhu Dongfang Cai Jianping Wang Jinhua Cao Yancheng Wen Junping He Lei Zhao Dongguo Wang Shufen Zhang | 2021 | Oil Crop Science2021，6，2： | 2 |
| 6 | Comprehensive two-dimensional HepG2/cell membrane chromatography/monolithic column/time-of-flight mass spectrometry system for screening anti-tumor components from herbal medicines显示文摘 | Xiaofei Chen Yan Cao Diya Lv Zhenyu Zhu Junping Zhang Yifeng Chai | 2012 | Journal of Chromatography A2012，，： | 1 |
| 7 | Isolation and Identification of a Porcine Pseudorabies Virus Strain in Taizhou City显示文摘In this study,the liver,kidney and spleen tissues were collected from pigs with suspected PR in a pig farm in Jiangyan District,Taizhou City for virus isolation and identification. The isolated virus was inoculated onto PK15 monolayer cells. The virus culture was collected to extract genomic DNA for PCR assay and indirect immunoinfluscent assay. The results showed that the isolated virus was porcine pseudorabies virus,which was named TAIZ130417. The growth titer of the isolated virus reached 108. 12 TCID50/ml on PK15 cells. Rabbits inoculated with the isolated virus soon exhibited pseudorabies symptoms such as itching and eventually died. The results provided reference for in-depth research and scientific prevention and control of pseudorabies. | Guangfu GUO Aiping ZHU Junping CAO Cailian JIN Lihong DAI | 2018 | Agricultural Biotechnology2018，7，5： | 1 |
| 8 | Type2d Iabetesandsyndromecharacter Ist Icsof显示文摘 | Wei Junp Ing Zhou LIbo LIu Fang Zhong Zhen LIu Ya jun Cao A Im ei Wang XIu zhi Zhu Yan Yu Bo LIu XIa Zhang Yanni To ng XIaol In | | 0，，12： | 1 |
| 9 | Sup- port vector machine based battery model for electric vehi- cles 显示文摘 | Wang Junping Chen Quanshi Cao Binggang | 2006 | Energy Conversion and Management2006，47，： | 1 |
| 10 | Glial Cell-Derived Neurotrophic Factor Attenuates Neuropathic Pain in a Mouse Model of Chronic Constriction Injury: Possible Involvement of E-cadherin/p120ctn Signaling显示文摘 | Cunjin Wang Hongjun Wang Jun Pang Li Li Suming Zhang Ge Song Na Li Junping Cao Licai Zhang | 2014 | Journal of Molecular Neuroscience2014，，2： | 1 |
| 11 | A Comparative Study of Supervised Learning as Applied to Acronym Expansion in Clinical Repo 显示文摘 | Mou Shumin Wei Junping Liu Fang Wan Xiuzhi Cao Aimei Liu Yajun Wang Jia Chen Liang Liu Do | 2008 | Sichuan2008，，11： | 1 |
| 12 | The clinical analysis of GAS:195 patients with digestive tract disease显示文摘 | Junping Ma Runlin Cao | | 0，，01： | 1 |
| 13 | Combined state of charge estimator for electric vehicle battery pack显示文摘 | WANG Junping CAO Binggang CHEN Quanshi | 2007 | Control Engineering Practice2007，15，12： | 1 |
| 14 | The clinical analysis of GAS:195patients with digestive tract disease显示文摘 | Junping Ma Runlin Cao | 2011 | Journal of ShanxiMedical College for Continuing Education2011，21，1： | 1 |
| 15 | The approximate age of the planation surface and the incision of the Yellow River显示文摘 | Baotian Pan Zhenbo Hu Junping Wang Jef Vandenberghe Xiaofei Hu Yuhua Wen Qiong Li Bo Cao | 2010 | Palaeogeography Palaeoclimatology Palaeoecology2010，，： | 1 |
| 16 | Optimum Application Time and Application Rate of Nitrogen Fertilizer in Brassica napus L.显示文摘To find proper nitrogen fertilizer application mode of rapeseed( Brassica napus L.),promote the high and stable yield of rapeseed,field experiment was carried out to study the effect of nitrogen fertilizer application modes on the yield and economic traits of Fengyou 10 rapeseed planted in two different fertility soils,and characteristics and differences of responses of different fertility soils to nitrogen fertilizer application modes were compared and analyzed. The results showed that the yield and related economic traits of rapeseed treated by nitrogen fertilizer were higher than the control group; in the nitrogen fertilizer treatment group,when the nitrogen fertilizer was applied as the base fertilizer and wintering fertilizer at the ratio of 7∶ 3,the rapeseed yield was higher than the control group and reached the highest; when the nitrogen fertilizer was applied as the base fertilizer,wintering fertilizer,and bud fertilizer at the ratio of 5∶ 3∶ 2,the rapeseed yield was significantly higher than the control group but was the lowest. These indicate that sufficient nitrogen supply is the basis for normal growth and development of rape seedlings. When the pure nitrogen fertilizer was at 192 kg/ha and the ratio of base fertilizer and wintering fertilizer was 7∶ 3,it can better coordinate the high yield of rapeseed and overall use of nitrogen fertilizer,and obtain higher rapeseed yield. | Junping HE Shufen ZHANG Jiacheng ZHU Jinhua CAO Yancheng WEN Dongfang CAI Lei ZHAO Dongguo WANG Jianping WANG | 2018 | Asian Agricultural Research2018，10，5： | 0 |
| 17 | Electromagnetic Radiation Mechanism Analysis and Field Strength Prediction of an Elementary Digital Inverter with Cables显示文摘In this paper,common mode(CM)and differential mode(DM)far-field radiation models of a typical digital inverter with cables are built up to predict electromagnetic field strength and analyze field characteristics.The CM current model and its analyses are based on the imbalance difference method.It is found out that the voltage between the drain and the source electrodes of upper transistor is the key equivalent source of electromagnetic interference(EMI).Far-field radiation strength of the digital inverter in free space is predicted by using the asymmetrical antenna radiation method and current loop radiation method.The accuracy of these methods is verified by the CST electromagnetic simulation results in the frequency range from 1 MHz to 400 MHz.Furthermore,the radiation models are improved by using the mirror method,which enables to include the reflection effect of the metal ground plane at the electromagnetic compatibility(EMC)test site.Both the results of measurements in a semi-anechoic chamber and the simulation results confirm the proposed electromagnetic radiation prediction method. | Junping He Xin Li Sili Tao Lingling Cao Xi Wang | 2022 | Chinese Journal of Electrical Engineering2022，8，3： | 0 |
| 18 | Stem cell properties and neural differentiation of sheep amniotic epithelial cells显示文摘This study was designed to verify the stem cell properties of sheep amniotic epithelial cells and their capacity for neural differentiation. Immunofluorescence microscopy and reverse transcription-PCR revealed that the sheep amniotic epithelial cells were positive for the embryonic stem cell marker proteins SSEA-1, SSEA-3, SSEA-4, TRA-1-60 and TRA-1-81, and the totipotency-associated genes Oct-4, Sox-2 and Rex-1, but negative for Nanog. Amniotic epithelial cells expressed β-III-tubulin, glial fibrillary acidic protein, nestin and microtubule-associated protein-2 at 28 days after induction with serum-free neurobasal-A medium containing B-27. Thus, sheep amniotic epithelial cells could differentiate into neurons expressing β-III-tubulin and microtubule-associated protein-2, and glial-like cells expressing glial fibrillary acidic protein, under specific conditions. | Xuemin Zhu Xiumei Wang Guifang Cao Fengjun Liu Yinfeng Yang Xiaonan Li Yuling Zhang Yan Mi Junping Liu Lingli Zhang | 2013 | Neural Regeneration Research2013，8，13： | 0 |
| 19 | N-cadherin expression in the midbrain substantia nigra of perinatal rats显示文摘BACKGROUND: Cellular adhesion molecule mediates the effects of glial cell line-derived neurotrophic factor in dopaminergic neurons during nervous system development. It is assumed that cellular adhesion molecule plays an important role in dopaminergic neuron development. OBJECTIVE: To investigate N-cadherin expression in the midbrain substantia nigra during rat development. DESIGN, TIME AND SETTING: The developmental biology, controlled study was performed at the Laboratory of Research Center of Neurobiology, Xuzhou Medical College, China from April to October 2007. MATERIALS: Sprague Dawley rats (embryonic days 16, 18, and 20, as well as postnatal days 1, 3, 7, 14, and 21) were selected for this study. N-cadherin antibody was purchased from Santa Cruz, USA. METHODS: N-cadherin expression in the midbrain substantia nigra of perinatal rats was detected using reverse transcription polymerase chain reaction, Western blot, and immunohistochemistry methods. N-cadherin-positive cells were counted. MAIN OUTCOME MEASURES: N-cadherin mRNA, protein, and positive cells in the rat midbrain substantia nigra were quantified. RESULTS: The results of reverse transcription polymerase chain reaction and Western blot demonstrated that altered N-cadherin mRNA levels were similar to protein levels in the midbrain substantia nigra. N-cadherin mRNA and protein expression was low in the midbrain substantia nigra of embryonic day 16 rats, but gradually increased, and reached a peak at postnatal day 1. N-cadherin mRNA and protein levels were still high at postnatal days 3 and 7, but significantly decreased at postnatal day 14. There was no significant difference in N-cadherin mRNA and protein expression between postnatal days 14 and 21 (P > 0.05). The immunohistochemistry results demonstrated that there was no significant difference in the quantity of N-cadherin-positive cells per area in rats from embryonic day 16 to postnatal day 7 (P > 0.05). The quantity of N-cadherin-positive cells per area was significantly decreased in rats at postnatal day 14. There was no significant difference in the number of N-cadherin-positive cells per area between postnatal days 14 and 21 (P > 0.05). CONCLUSION: N-cadherin expression was reduced during embryonic stages, but reached a peak at postnatal days 1-7, and then gradually decreased in the rat midbrain substantia nigra. | Junping Cao Yu Sun Xia Du Dianshuai Gao | 2008 | Neural Regeneration Research2008，3，10： | 0 |
| 20 | Isolation and Identification of Porcine Circovirus Type 2 in Taizhou显示文摘In September 2011,an infectious disease suspected to be postweaning multisystemic wasting syndrome( PMWS) broke out in some pig farm in Taizhou.The inguinal lymph node,liver and lung tissues were collected and grinded into tissue suspension. The suspension was subjected to PCR detection,and the positive product was sequenced. The suspension of positive samples was filtered with 0. 22 μm filter membrane,and the filtrate was inoculated onto PK15 cells. After five generations of blind passages,the cell viral liquid was collected and extracted for DNA,which was subjected to PCR detection and indirect immunofluorescence.The results showed that the isolate was porcine circovirus type 2( PCV2) and designated as TAIZ110926. The target sequence was committed to NCBI with a serial number: KF039888. | Guangfu GUO Aiping ZHU Junping CAO Cailian JIN Lihong DAI Hongwei TAO | 2018 | Agricultural Biotechnology2018，7，4： | 0 |
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