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| 1 | Application of next-generation sequencing technology to precision medicine in cancer: joint consensus of the Tumor Biomarker Committee of the Chinese Society of Clinical Oncology显示文摘Next-generation sequencing(NGS) technology is capable of sequencing millions or billions of DNA molecules simultaneously.Therefore, it represents a promising tool for the analysis of molecular targets for the initial diagnosis of disease, monitoring of disease progression, and identifying the mechanism of drug resistance. On behalf of the Tumor Biomarker Committee of the Chinese Society of Clinical Oncology(CSCO) and the China Actionable Genome Consortium(CAGC), the present expert group hereby proposes advisory guidelines on clinical applications of NGS technology for the analysis of cancer driver genes for precision cancer therapy. This group comprises an assembly of laboratory cancer geneticists, clinical oncologists, bioinformaticians,pathologists, and other professionals. After multiple rounds of discussions and revisions, the expert group has reached a preliminary consensus on the need of NGS in clinical diagnosis, its regulation, and compliance standards in clinical sample collection. Moreover, it has prepared NGS criteria, the sequencing standard operation procedure(SOP), data analysis, report, and NGS platform certification and validation. | Xuchao Zhang Zhiyong Liang Shengyue Wang Shun Lu Yong Song Ying Cheng Jianming Ying Weiping Liu Yingyong Hou Yangqiu Li Yi Liu Jun Hou Xiufeng Liu Jianyong Shao Yanhong Tai Zheng Wang Li Fu Hui Li Xiaojun Zhou Hua Bai Mengzhao Wang You Lu Jinji Yang Wenzhao Zhong Qing Zhou Xuening Yang Jie Wang Cheng Huang Xiaoqing Liu Xiaoyan Zhou Shirong Zhang Hongxia Tian Yu Chen Ruibao Ren Ning Liao Chunyan Wu Zhongzheng Zhu Hongming Pan Yanhong Gu Liwei Wang Yunpeng Liu Suzhan Zhang Tianshu Liu Gong Chen Zhimin Shao Binghe Xu Qingyuan Zhang Ruihua Xu Lin Shen Yilong Wu | 2019 | Cancer Biology & Medicine2019，16，1： | 14 |
| 2 | Bevacizumab biosimilar LY01008 compared with bevacizumab(Avastin)as first-line treatment for Chinese patients with unresectable,metastatic,or recurrent non-squamous non-small-cell lung cancer:A multicenter,randomized,double-blinded,phase Ⅲ trial显示文摘Background:Previous studies have demonstrated the preclinical pharmacological and toxicological consistency,and clinical pharmacokinetic equivalence of bevacizumab biosimilar LY01008 with reference bevacizumab(Avastin).This randomized controlled trial aimed to compare the efficacy and safety of LY01008 with Avastin in first-line treatment of Chinese patients with advanced or recurrent non-squamous non-small cell lung cancer(NSCLC).Methods:StageⅢB-ⅣNSCLC patients with evaluable lesions,good physical status,and adequate organ functions from 67 centers across China were randomized in a ratio of 1:1 to receive LY01008 or Avastin 15 mg/kg intravenously in combination with paclitaxel/carboplatin(combined treatment)for 4-6 cycles,followed by maintenance monotherapy with LY01008 until disease progression,intolerable toxicity,or death.The primary endpoint was objective response rate(ORR)in accordance with Response Evaluation Criteria in Solid Tumors(RECIST)version 1.1 confirmed by independent radiological review committees(IRRC).Secondary endpoints included disease control rate(DCR),duration of response(DoR),progression-free survival(PFS),overall survival(OS),and safety.This study was registered in Clinical Trials.gov(NCT03533127).Results:Between December 15^(th),2017,and May 15^(th),2019,a total of 649 patients were randomized to the LY01008(n=324)or Avastin(n=325)group.As of September 25th,2019 for primary endpoint analysis,589 patients received ORR evaluation,with a median number of combined treatment cycles of 5(range 1-6)andmedian duration of treatment of 3.0(range 0.0-5.1)months.ORRof responseevaluable patients in the LY01008 and Avastin groups were 48.5% and 53.0%,respectively.The stratified ORR ratio was 0.91(90%CI 0.80-1.04,within the prespecified equivalence margin of 0.75-1.33).Up to May 15^(th),2020,with a median follow-up of 13.6(range 0.8-28.4)months,no notable differences in DCR,median DoR,median PFS,median OS,and 1-year OS rate were observed between the LY01008 and Avastin groups.There were no clinically meaningful differences in safety and immunogenicity across treatment groups.Conclusions:LY01008 demonstrated similarity to Avastin in terms of efficacy and safety in Chinese patients with advanced or recurrent non-squamous NSCLC.LY01008 combined with paclitaxel/carboplatin is expected to become a new treatment option for unresectable,metastatic,LY01008 and Avastin groups.There were no clinically meaningful differences in safety and immunogenicity across treatment groups.Conclusions:LY01008 demonstrated similarity to Avastin in terms of efficacy and safety in Chinese patients with advanced or recurrent non-squamous NSCLC.LY01008 combined with paclitaxel/carboplatin is expected to become a new treatment option for unresectable,metastatic,or recurrent non-squamous NSCLC patients in the first-line setting. | Yuankai Shi Kaijian Lei Yuming Jia Bingqiang Ni Zhiyong He Minghong Bi Xicheng Wang Jianhua Shi Ming Zhou Qian Sun Guolei Wang Dongji Chen Yongqian Shu Lianke Liu Zhongliang Guo Yong Liu Junquan Yang Ke Wang Ke Xiao LinWu Tienan Yi Debin Sun Mafei Kang Tianjiang Ma Yimin Mao Jinsheng Shi Tiegang Tang Yan Wang Puyuan Xing Dongqing Lv Wangjun Liao Zhiguo Luo Bin Wang Xiaohong Wu Xiaoli Zhu Shuhua Han Qisen Guo Rongyu Liu Zhiwei Lu Jianyong Zhang Jian Fang Changlu Hu Yinghua Ji Guolong Liu Hong Lu Dedong Wu Junhong Zhang Shuyang Zhu Zheng Liu Wensheng Qiu Feng Ye Yan Yu Yanqiu Zhao Qinhong Zheng Jun Chen Zhanyu Pan Yiping Zhang Wenjuan Lian Bo Jiang Bo Qiu Guojun Zhang Hua Zhang Yanju Chen Yuan Chen Hongbing Duan Manxiang Li Shengming Liu Lijun Ma Hongming Pan Xia Yuan Xueli Yuan Yulong Zheng Emei Gao Li Zhao Shumin Wang Can Wu | 2021 | Cancer Communications2021，41，9： | 5 |
| 3 | Gut Microbiota Dysbiosis Strengthens Kupffer Cell-mediated Hepatitis B Virus Persistence through Inducing Endotoxemia in Mice显示文摘Background and Aims:Change of gut microbiota com-position is associated with the outcome of hepatitis B virus(HBV)infection,yet the related mechanisms are not fully characterized.The objective of this study was to investigate the immune mechanism associated with HBV persistence induced by gut microbiota dysbiosis.Methods:C57BL/6 mice were sterilized for gut-microbiota by using an antibi-otic(ABX)mixture protocol,and were monitored for their serum endotoxin(lipopolysaccharide[LPS])levels.An HBV-replicating mouse model was established by performing HBV-expressing plasmid pAAV/HBV1.2 hydrodynamic injec-tion(HDI)with or without LPS,and was monitored for se-rum hepatitis B surface antigen,hepatitis B e antigen,HBV DNA,and cytokine levels.Kupffer cells(KCs)were purified from antibiotic-treated mice and HBV-replicating mice and analyzed for IL-10 production and T cell suppression ability.Results:ABX treatment resulted in increased serum LPS levels in mice.The KCs separated from both ABX-treated and LPS-treated HBV-replicating mice showed significantly increased IL-10 production and enhanced ability to suppress IFN-γproduction of TCR-activated T cells than the KCs sep-arated from their counterpart controls.HDI of pAAV/HBV1.2 in combination with LPS in mice led to a delayed HBV clear-ance and early elevation of serum IL-10 levels compared to pAAV/HBV1.2 HDI alone.Moreover,IL-10 function blockade or KC depletion led to accelerated HBV clearance in LPS-treated HBV-replicating mice.Conclusions:Our results suggest that dysbiosis of the gut microbiota in mice leads to endotoxemia,which induces KC IL-10 production and strengthens KC-mediated T cell suppression,and thus fa-cilitates HBV persistence. | Wenqing Zhou Jinzhuo Luo Xiaohong Xie Shangqing Yang Dan Zhu Hongming Huang Dongliang Yang Jia Liu | 2022 | Journal of Clinical and Translational Hepatology2022，10，1： | 4 |
| 4 | Peniazaphilones A—I,Produced by Co-culturing of Mangrove Endophytic Fungi,Penicillium sclerotiorum THSH-4 and Penicillium sclerotiorum ZJHJJ-18显示文摘Co-culturing the mangrove endophytic fungus Penicillium sclerotiorum THSH-4 with Penicillium sclerotiorum ZJHJJ-18 in PDB medium resulted in the production of nine new azaphilones derivatives,peniazaphilones A—I(1-9),and six known azaphilones derivatives(10-15).Their structures were elucidated by analysis of detailed spectroscopic data,single-crystal X-ray diffraction,ECD spectra,^(13)C NMR calculation and DP4+analysis.It is noteworthy that compound 1 with an isoquinoline quinone moiety,was isolated from natural source for the first time.Besides,compound 2 is the third reported phenylhydrazone derivative from fungi.In the bioactivity assays,compounds 1,12,14 and 15(IC_(50)=4.71-17.64μmol/L)exhibited potent inhibition of LPS-induced NO release from RAW264.7 without obvious cytotoxicity within 50μmol/L compared to the positive control indomethacin(IC_(50)=35.27μmol/L).Moreover,compounds 1 and 13 displayed moderate to strong cytotoxicity to A549 and MDA-MB-435,with IC_(50) values in the range of 6.84-14.63μmol/L.Together compound 1 showed moderate broad-spectrum antibacterial activity. | Wencong Yang Jie Yuan Qi Tan Yan Chen Yujia Zhu Hongming Jiang Ge Zou Zhenming Zang Bo Wang Zhigang She | 2021 | Chinese Journal of Chemistry2021，39，12： | 3 |
| 5 | Analysis of strand breakage caused by conductor vibration in transmission lines显示文摘I. Introduction A 31.2 km 110 kV transmission line is designed to operateunder 35 kV with conductor model AC-185 (aluminium strand-- 28 x 2.87 mm^2, steel core -- 7 x 2.50 mm^2) at average oper-ating stress of 24. 2%, and overhead ground wire model C-50(steel strand -- 7 x 2.97 mm^2) at average operating stress of20.6%. The whole line has 130 pole-towers, most of which are ofprestressed concrete double-pole structure, as shown in Fig.1. | Zhu Zhongming Suzhou Thermal Power Research Institute Shen Hongming | 1992 | Electricity1992，，2： | 2 |
| 6 | Neural mechanism of proposer's decision-making in the ultimatum and dictator games显示文摘Previous studies have demonstrated that reactions to unfair offers in the ultimatum game are correlated with negative emotion.However,little is known about the difference in neural activity between a proposer's decision-making in the ultimatum game compared with the dictator game.The present functional magnetic resonance imaging study revealed that proposing fair offers in the dictator game elicited greater activation in the right supramarginal gyrus,right medial frontal gyrus and left anterior cingulate cortex compared with proposing fair offers in the ultimatum game in 23 Chinese undergraduate and graduate students from Beijing Normal University in China.However,greater activation was found in the right superior temporal gyrus and left cingulate gyrus for the reverse contrast.The results indicate that proposing fair offers in the dictator game is more strongly associated with cognitive control and conflicting information processing compared with proposing fair offers in the ultimatum game. | Hongming Zheng Liqi Zhu | 2013 | Neural Regeneration Research2013，8，4： | 2 |
| 7 | Advances in antibiotic therapy for infection after the surgical installation of implants to treat internal fractures显示文摘To summarize the advances in antibiotic therapy for infection after the surgical installation of implants to treat internal fractures. Recent studies on antibiotic therapy for infection after the surgical installation of implants to internal fractures were reviewed and analyzed.In general, systematic antibiotics are selected based on the results of bacterial culture. The duration of antibiotic treatment lasts for no more than 4 to 6 weeks. Orally administered and intravenously injected antibiotics have similar efficacies. Orally administered antibiotics exhibit a lower incidence of complications and are less costly than intravenously injected antibiotics. In addition, the efficacy of daptomycin in the treatment of bone infection is problematic. Rifampicin or fluoroquinolone antibiotics should be jointly administered when infection with bacterial biofilms is likely to occur. Calcium sulfate is a typical topically applied antibiotic delivery vehicle that can be completely degraded, with good biocompatibility, bone conduction, and drug release. The rational, systematic, and combined topical application of antibiotics can effectively decrease the recurrence rates of infection after the surgical installation of implants to treat internal fractures and can improve the quality of life of patients. | Yue Sun Ying Liu Hongming Zhu | 2016 | 国际感染病学(电子版)2016，5，3： | 2 |
| 8 | Therapeutic silencing miR-146b-5p improves cardiac remodeling in a porcine model of myocardial infarction by modulating the wound reparative phenotype显示文摘Fibrotic remodeling is an adverse consequence of immune response-driven phenotypic modulation of cardiac cells following myocardial infarction(Ml).MicroRNA-146b(miR-146b)is an active regulator of immunomodulation,but its function in the cardiac inflammatory cascade and its clinical implication in fibrotic remodeling following Ml remain largely unknown.Herein,miR-146b-5p was found to be upregulated in the infarcted myocardium of mice and the serum of myocardial ischemia patients.Gain-and loss-of-function experiments demonstrated that miR-146b-5p was a hypoxia-induced regulator that governed the pro-fibrotic phenotype transition of cardiac cells.Overexpression of miR-146b-5p activated fibroblast proliferation,migration,and fibroblast-to-myofibroblast transition,impaired endothelial cell function and stress survival,and disturbed macrophage paracrine signaling.Interestingly,the opposite effects were observed when miR-146b-5p expression was inhibited.Luciferase assays and rescue studies demonstrated that the miR-146b-5p target genes mediating the above phenotypic modulations included interleukin 1 receptor associated kinase 1(IRAKI)and carcinoembryonic antigen related cell adhesion molecule 1(CEACAM1).Local delivery of a miR-146b-5p antagomir significantly reduced fibrosis and cell death,and upregulated capillary and reparative macrophages in the infarcted myocardium to restore cardiac remodeling and function in both mouse and porcine Ml models.Local inhibition of miR-146b-5p may represent a novel therapeutic approach to treat cardiac fibrotic remodeling and dysfunction following Ml. | Yiteng Liao Hao Li Hao Cao Yun Dong Lei Gao Zhongmin Liu Junbo Ge Hongming Zhu | 2021 | Protein & Cell2021，12，3： | 1 |
| 9 | Intercalated combination of chemotherapy and erlotinib for patients with advanced stage non-small-cell lung cancer (FASTACT-2): a randomised, double-blind trial显示文摘 | Yi-Long Wu Jin Soo Lee Sumitra Thongprasert Chong-Jen Yu Li Zhang Guia Ladrera Vichien Srimuninnimit Virote Sriuranpong Jennifer Sandoval-Tan Yunzhong Zhu Meilin Liao Caicun Zhou Hongming Pan Victor Lee Yuh-Min Chen Yan Sun Benjamin Margono Fatima Fuerte | 2013 | Lancet Oncology2013，，8： | 1 |
| 10 | Intercalated combination of chemotherapy and erlotinib for patients with advanced stage non-small-cell lung cancer (FASTACT-2): a randomised, double-blind trial显示文摘 | Yi-Long Wu Jin Soo Lee Sumitra Thongprasert Chong-Jen Yu Li Zhang Guia Ladrera Vichien Srimuninnimit Virote Sriuranpong Jennifer Sandoval-Tan Yunzhong Zhu Meilin Liao Caicun Zhou Hongming Pan Victor Lee Yuh-Min Chen Yan Sun Benjamin Margono Fatima Fuerte | 2013 | Lancet Oncology2013，，8： | 1 |
| 11 | SNP Cutter: a comprehensive tool for SNP PCR-RFLP assay de- sign显示文摘 | Zhang Ruifang Zhu Zanhua Zhu Hongming | 2005 | Nucleic Acids Res2005，33，2： | 1 |
| 12 | Intercalated combination of chemotherapy and erlotinib for patients with advanced stage non-small-cell lung cancer (FASTACT-2): a randomised, double-blind trial显示文摘 | Yi-Long Wu Jin Soo Lee Sumitra Thongprasert Chong-Jen Yu Li Zhang Guia Ladrera Vichien Srimuninnimit Virote Sriuranpong Jennifer Sandoval-Tan Yunzhong Zhu Meilin Liao Caicun Zhou Hongming Pan Victor Lee Yuh-Min Chen Yan Sun Benjamin Margono Fatima Fuerte | 2013 | Lancet Oncology2013，，8： | 1 |
| 13 | Macrophage membrane-biomimetic adhesive polycaprolactone nanocamptothecin for improving cancer-targeting efficiency and impairing metastasis显示文摘The recent remarkable success and safety of mRNA lipid nanoparticle technology for producing severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)vaccines has stimulated intensive efforts to expand nanoparticle strategies to treat various diseases.Numerous synthetic nanoparticles have been developed for pharmaceutical delivery and cancer treatment.However,only a limited number of nanotherapies have enter clinical trials or are clinically approved.Systemically administered nanotherapies are likely to be sequestered by host mononuclear phagocyte system(MPS),resulting in suboptimal pharmacokinetics and insufficient drug concentrations in tumors.Bioinspired drug-delivery formulations have emerged as an alternative approach to evade the MPS and show potential to improve drug therapeutic efficacy.Here we developed a biodegradable polymer-conjugated camptothecin prodrug encapsulated in the plasma membrane of lipopolysaccharide-stimulated macrophages.Polymer conjugation revived the parent camptothecin agent(e.g.,7-ethyl-10-hydroxy-camptothecin),enabling lipid nanoparticle encapsulation.Furthermore,macrophage membrane cloaking transformed the nonadhesive lipid nanoparticles into bioadhesive nanocamptothecin,increasing the cellular uptake and tumor-tropic effects of this biomimetic therapy.When tested in a preclinical murine model of breast cancer,macrophage-camouflaged nanocamptothecin exhibited a higher level of tumor accumulation than uncoated nanoparticles.Furthermore,intravenous administration of the therapy effectively suppressed tumor growth and the metastatic burden without causing systematic toxicity.Our study describes a combinatorial strategy that uses polymeric prodrug design and cell membrane cloaking to achieve therapeutics with high efficacy and low toxicity.This approach might also be generally applicable to formulate other therapeutic candidates that are not compatible or miscible with biomimetic delivery carriers. | Kangkang Ying Yifeng Zhu Jianqin Wan Chenyue Zhan Yuchen Wang Binbin Xie Peirong Xu Hongming Pan Hangxiang Wang | 2023 | Bioactive Materials2023，，2： | 1 |
| 14 | Topological phases in pyrochlore thallium niobate Tl_(2)Nb_(2)O_(6+x)显示文摘The discovery of new topological electronic materials brings a chance to uncover new physics.Up to now,many materials have been theoretically proposed and experimentally proved to host different kinds of topological states.Unfortunately,there is little convincing experimental evidence for the existence of topological oxides.The reason is that oxidation of oxygen leads to ionic crystal in general and makes band inversion unlikely.In addition,the realization of different topological states in a single material is quite difficult,but strongly needed for exploring topological phase transitions. | Wei Zhang Kaifa Luo Zhendong Chen Ziming Zhu Rui Yu Chen Fang Hongming Weng | 2019 | npj Computational Materials2019，，1： | 0 |
| 15 | Elementary introduction to the relevant elements and measures of green building design显示文摘With the continuous development of the concept of green architecture,the integration of a large number of environmental protection concepts in the architectural design is the focus of green architecture design.Saving natural resources can also promote the harmonious development of man and nature.Green building design concept to meet the needs of people at the same time,promote the harmonious development of man and nature.Therefore,the concept of green architecture should be fully used in the design of architecture. | Junguo Yang Hongming Zhu Shulong Zhang Suju Xia Li Liu | 2018 | Smart Construction Research2018，2，1： | 0 |
| 16 | Meta Ordinal Regression Forest for Medical Image Classification With Ordinal Labels显示文摘The performance of medical image classification has been enhanced by deep convolutional neural networks(CNNs),which are typically trained with cross-entropy(CE)loss.However,when the label presents an intrinsic ordinal property in nature,e.g.,the development from benign to malignant tumor,CE loss cannot take into account such ordinal information to allow for better generalization.To improve model generalization with ordinal information,we propose a novel meta ordinal regression forest(MORF)method for medical image classification with ordinal labels,which learns the ordinal relationship through the combination of convolutional neural network and differential forest in a meta-learning framework.The merits of the proposed MORF come from the following two components:A tree-wise weighting net(TWW-Net)and a grouped feature selection(GFS)module.First,the TWW-Net assigns each tree in the forest with a specific weight that is mapped from the classification loss of the corresponding tree.Hence,all the trees possess varying weights,which is helpful for alleviating the tree-wise prediction variance.Second,the GFS module enables a dynamic forest rather than a fixed one that was previously used,allowing for random feature perturbation.During training,we alternatively optimize the parameters of the CNN backbone and TWW-Net in the meta-learning framework through calculating the Hessian matrix.Experimental results on two medical image classification datasets with ordinal labels,i.e.,LIDC-IDRI and Breast Ultrasound datasets,demonstrate the superior performances of our MORF method over existing state-of-the-art methods. | Yiming Lei Haiping Zhu Junping Zhang Hongming Shan | 2022 | IEEE/CAA Journal of Automatica Sinica2022，9，7： | 0 |
| 17 | Rapid identification of diarrheagenic Escherichia coli based on barcoded magnetic bead hybridization显示文摘Diarrhea,as a global public health problem,causes a large number of infections and deaths every year.Although Escherichia coli(E.coli)is one of the normal flo ra microorganisms in the human intestinal tract,it has five pathogenic bacteria types that can cause human diarrhea,known as diarrheagenic E.coli.When people are infected,rapid and accurate diagnosis,along with timely treatment,are especially important.Here,we introduce a new method to identify and analyze a large number of pathogenic strains in E.coli by multiplex PCR and barcoded magnetic bead hybridization.Results show that the detection sensitivities of enterohemorrhagic E.coli,enterotoxigenic E.coli,enteropathogenic E.coli,enteroinvasive E.coli and enteroaggregative E.coli were 1.3×10^3 CFU/mL,2×10^4 CFU/mL,4×10^4 CFU/mL,7.2×10^4 CFU/mL and 1.7 CFU/mL respectively.This method has strong specificity and high sensitivity and detects multiple target sequences in one experiment.Compared with other methods,BMB array has great application potential. | Hongming Dong Congli Tang Ziyu He Hongmei Liu Yuyue Xu Hao Huang Gaojian Yang Ziqi Xiao Song Li Yan Deng Zhu Chen Hui Chen Zuodong Qin Yasser Perera Negrin Nongyue He | 2020 | Chinese Chemical Letters2020，31，7： | 0 |
| 18 | Three Dimensional Metal-Surface Processing Parameter Generation Through Machine Learning-Based Nonlinear Mapping显示文摘The accuracy and efficiency of three-dimensional(3D)surface forming,which directly affects the cycle and quality of production,is important in manufacturing.In practice,given the uncertainty of metal plate springback,an error exists between the actual plate and the target surface,which creates a nonlinear mapping from computer aided design models to bending surfaces.Technicians need to reconfigure parameters and process a surface multiple times to delicately control springback,which greatly wastes human and material resources.This study aims to address the springback control problem to improve the efficiency and accuracy of sheet metal forming.A basic computation approach is proposed based on the DeepFit model to calculate the springback value in 3D surface bending.To address the sample data shortage problem,we put forward an advanced approach by combining a deep learning model with case-based reasoning(CBR).Next,a multi-model fused bending parameter generation framework is devised to implement the advanced springback computation approach through surface data preprocessing,CBR-based model matching,convolution neural network-based machining surface generation,and bending parameter generation with a series of model transformations.Moreover,the proposed approaches and the framework are verified by considering saddle surface processing as an example.Overall,this study provides a new idea to improve the accuracy and efficiency of surface processing. | Min Zhu Yanjun Dong Bingqing Shen Haiyan Yu Lihong Jiang Hongming Cai | 2023 | Tsinghua Science and Technology2023，28，4： | 0 |
| 19 | Predictive values of plasma TNFαand IL-8 for intracranial hemorrhage in patients with acute promyelocytic leukemia显示文摘In patients with acute promyelocytic leukemia(APL),intracranial hemorrhage(ICH),if not identified promptly,could be fatal.It is the leading cause of failure of induction and early death.Thus,biomarkers that could promptly predict severe complications are critical.Here,cytokine differences between patients with APL with and without ICH were investigated to develop predictive models for this complication.The initial cytokine profiling using plasma samples from 39 patients and 18 healthy donors found a series of cytokines that were remarkedly different between patients with APL and healthy controls.The APL patients were subsequently divided into high and low white blood cell count groups.Results showed that tumor necrosis factor a and interleukin 8(IL-8)were vital in distinguishing patients with APL who did or did not develop ICH.In addition,verification in 81 patients with APL demonstrated that the two cytokines were positively correlated with the cumulative incidence of ICH.Finally,in-vitro and in-vivo experimental evidence were provided to show that IL-8 influenced the migration of APL-derived NB4 cells and impaired the blood-brain barrier in PML/RARαpositive blast-transplanted FVB/NJ mice.These assessments may facilitate the early warning of ICH and reduce future mortality levels in APL. | Fangyi Dong Li Chen Chaoxian Zhao Xiaoyang Li Yun Tan Huan Song Wen Jin Hongming Zhu Yunxiang Zhang Kai Xue Junmin Li Kankan Wang | 2022 | Frontiers of Medicine2022，16，6： | 0 |
| 20 | Extracellular vesicles from human urine-derived stem cells delay aging through the transfer of PLAU and TIMP1显示文摘Aging increases the risks of various diseases and the vulnerability to death.Cellular senescence is a hallmark of aging that contributes greatly to aging and aging-related diseases.This study demonstrates that extracellular vesicles from human urine-derived stem cells(USC-EVs)efficiently inhibit cellular senescence in vitro and in vivo.The intravenous injection of USC-EVs improves cognitive function,increases physical fitness and bone quality,and alleviates aging-related structural changes in different organs of senescence-accelerated mice and natural aging mice.The anti-aging effects of USC-EVs are not obviously affected by the USC donors’ages,genders,or health status.Proteomic analysis reveals that USC-EVs are enriched with plasminogen activator urokinase(PLAU)and tissue inhibitor of metalloproteinases 1(TIMP1).These two proteins contribute importantly to the anti-senescent effects of USC-EVs associated with the inhibition of matrix metalloproteinases,cyclin-dependent kinase inhibitor 2A(P16INK4a),and cyclin-dependent kinase inhibitor 1A(P21cip1).These findings suggest a great potential of autologous USC-EVs as a promising anti-aging agent by transferring PLAU and TIMP1 proteins. | Shanshan Rao Zehui He Zun Wang Hao Yin Xiongke Hu Yijuan Tan Tengfei Wan Hao Zhu Yi Luo Xin Wang Hongming Li Zhenxing Wang Xinyue Hu Chungu Hong Yiyi Wang Mingjie Luo Wei Du Yuxuan Qian Siyuan Tang Hui Xie Chunyuan Chen | 2024 | Acta Pharmaceutica Sinica B2024，14，3： | 0 |
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