|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Effect of acetyl-L-carnitine on hypersensitivity in acute recurrent caerulein-induced pancreatitis and microglial activation along the brain’s pain circuitry显示文摘BACKGROUND Acute pancreatitis(AP)and recurring AP are serious health care problems causing excruciating pain and potentially lethal outcomes due to sepsis.The validated caerulein-(CAE)induced mouse model of acute/recurring AP produces secondary persistent hypersensitivity and anxiety-like behavioral changes for study.AIM To determine efficacy of acetyl-L-carnitine(ALC)to reduce pain-related behaviors and brain microglial activation along the pain circuitry in CAE-pancreatitis.METHODS Pancreatitis was induced with 6 hly intraperitoneal(i.p.)injections of CAE(50μg/kg),3 d a week for 6 wk in male C57BL/6J mice.Starting in week 4,mice received either vehicle or ALC until experiment’s end.Mechanical hypersensitivity was assessed with von Frey filaments.Heat hypersensitivity was determined with the hotplate test.Anxiety-like behavior was tested in week 6 using elevated plus maze and open field tests.Microglial activation in brain was quantified histologically by immunostaining for ionized calcium-binding adaptor molecule 1(Iba1).RESULTS Mice with CAE-induced pancreatitis had significantly reduced mechanical withdrawal thresholds and heat response latencies,indicating ongoing pain.Treatment with ALC attenuated inflammation-induced hypersensitivity,but hypersensitivity due to abdominal wall injury caused by repeated intraperitoneal injections persisted.Animals with pancreatitis displayed spontaneous anxiety-like behavior in the elevated plus maze compared to controls.Treatment with ALC resulted in increased numbers of rearing activity events,but time spent in“safety”was not changed.After all the abdominal injections,pancreata were translucent if excised at experiment’s end and opaque if excised on the subsequent day,indicative of spontaneous healing.Post mortem histopathological analysis performed on pancreas sections stained with Sirius Red and Fast Green identified wide-spread fibrosis and acinar cell atrophy in sections from mice with CAE-induced pancreatitis that was not rescued by treatment with ALC.Microglial Iba1 immunostaining was significantly increased in hippocampus,thalamus(intralaminar nuclei),hypothalamus,and amygdala of mice with CAE-induced pancreatitis compared to naïve controls but unchanged in the primary somatosensory cortex compared to naïves.CONCLUSION CAE-induced pancreatitis caused increased pain-related behaviors,pancreatic fibrosis,and brain microglial changes.ALC alleviated CAE-induced mechanical and heat hypersensitivity but not abdominal wall injury-induced hypersensitivity caused by the repeated injections. | Sabrina L McIlwrath Marlene E Starr Abigail E High Hiroshi Saito Karin N Westlund | 2021 | World Journal of Gastroenterology2021，27，9： | 2 |
| 2 | Antitumour triterpenes of Maytenus diversifolia 显示文摘 | Hiroshi N Hideyo S Teruhisa H | 1986 | Phytochemistry1986，25，2： | 1 |
| 3 | Introduction on the protective gas of the new GWP≈1 magnesium alloy显示文摘 | Hiroshi S Yuuji N Masakazu K | 2004 | Materia2004，43，4： | 1 |
| 4 | Antiatherogenic activity of fungal beauveriolides,inhibitors of lipid droplet accumulation in macrophages显示文摘 | Ichiji N Hiroshi T Shun I | 2004 | Biochemistry2004，101，3： | 1 |
| 5 | Restrictive versus liberal blood transfusion policy for hepatectomies in cirrhotic patients显示文摘 | Masatoshi Makuuchi M.D. Tadatoshi Takayama M.D. Peter Gunvén M.D. Tomoo Kosuge M.D. Susumu Yamazaki M.D. Hiroshi Hasegawa M.D | 1989 | World Journal of Surgery1989，，5： | 1 |
| 6 | Inflammatory pseudotumor of the liver in a patient with early gastric cancer: CT-histopathological correlation 显示文摘 | Rieko N Hiroshi M Norihiro T | 2005 | Jpn J Clin Oneol2005，35，4： | 1 |
| 7 | Behavior of Balls in a Standard Ball Reducer显示文摘 | Hiroyuki O Yusuke M Hiroshi N | 2002 | Proceedings of the Japan Society of Mechanical Engineers2002，674，68： | 1 |
| 8 | RANKL-RANK signaling in osteoclastogenesis and bone disease显示文摘 | Wada T Nakashima T Hiroshi N | | 0，，01： | 1 |
| 9 | Decomposition mechanism of organic compounds by DC water plasmas at atmospheric pressure显示文摘 | HIROSHI N HIRONORI S TAKAYUKI W | 2009 | Thin solid films2009，，518： | 1 |
| 10 | Methylation status of breast cancer resistance protein detected by methylation-specific polymerase chain reaction analysis is correlated inversely with its expression in drug resistant lung cancer cells显示文摘 | Hirofumi N Yoichi N Hiroshi S | 2008 | Cancer2008，112，5： | 1 |
| 11 | RANKL-RANK signaling in osteoclastogenesis and bone disease显示文摘 | Wada T Nakashima T Hiroshi N | 2006 | Trends Mol Med2006，12，1： | 1 |
| 12 | Comparison of lasing performance of Tm,Ho:YLF laser by use of single and double cavities显示文摘 | JUN I HAYATO N HIROSHI H | 2000 | Appl Opt2000，39，15： | 1 |
| 13 | Mulberry latex rich in antidiabetic sugar-mimic alkaloids forces dieting on caterpillars显示文摘 | Kotaro K Hiroshi O Masatoshi N | 2006 | Proc Natl Acad Sci USA2006，103，5： | 1 |
| 14 | Characteristics of ion beam magnesium oxide films显示文摘 | NOBUTO Y HIROSHI N ARI I | 2004 | Thin Solid Film2004，，447448： | 1 |
| 15 | Inhibitory effects of Chrysanthemum species extracts on formation of advancedg|ycation end products显示文摘 | Kentaro T N Hiroshi S Miyuki H | 2009 | Food Chemistry2009，116，5： | 1 |
| 16 | Hemodynamic compromiseas a cause of Internal border-zone infarction and cortical neuronaldamage in,atherosclerotic middle cerebral artery disease 显示文摘 | Hiroshi Y Ryuichi N Tatsuya H | 2009 | Stroke2009，40，： | 1 |
| 17 | RANKL-RANK signaling in osteoclastogenesis and bone disease显示文摘 | Wada T Nakashima T Hiroshi N | | 0，，01： | 1 |
| 18 | HRCT findings of bronchiolitis obliterans organizing pneumonia显示文摘 | Kunihiro Y Yasuyuki K Hiroshi N | 2001 | The Radiologist2001，8，4： | 1 |
| 19 | Implication of cardiac remodeling in heart falure: mochanismsand therapeutic strategies显示文摘 | Hiroyuki T Hiroshi H Toshio N | 2003 | Internal Medicine2003，42，： | 1 |
| 20 | Squalene as novel food factor显示文摘 | Hari N B Naoto T Hiroshi N | 2010 | Curr Pharm Biotechnol2010，9，： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员