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| 1 | Endostatin enhances antitumor effect of tumor antigen-pulsed dendritic cell therapy in mouse xenograft model of lung carcinoma显示文摘Objective: To investigate the antitumor effect of endostatin combined with tumor antigen-pulsed dendritic cell(DC)-T cell therapy on lung cancer.Methods: Transplanted Lewis lung cancer(LLC) models of C57BL/6 mice were established by subcutaneous injection of LLC cells in left extremity axillary. Tumor antigen-pulsed DC-T cells from spleen cells and bone of mice were cultured in vitro. Tumor-bearing mice were randomly divided into three groups, including DCT+endostatin group, DC-T group, and phosphate-buffered saline(PBS) control group. Microvessel density(MVD) of tumor tissue in tumor-bearing mice was determined by immunohistochemistry(IHC). The expressions of vascular endothelial growth factor(VEGF) and hypoxia-inducible factor-1α(HIF-1α) were determined by Western blotting and IHC staining. The proportions of CD8+ T cells, mature dendritic cells(m DC), tumor-associated macrophages [TAM(M1/M2)], and myeloid-derived suppressor cells(MDSC) in suspended cells of tumor tissue were determined by flow cytometry. The expressions of interleukin(IL)-6, IL-10, IL-17, transforming growth factor-β(TGF-β) and interferon-γ(IFN-γ) in suspended cells of tumor tissue were detected by enzyme-linked immune sorbent assay(ELISA).Results: DC-T cells combined with endostatin remarkably suppressed tumor growth. MVD of mice in DCT+endostatin group was significantly lower than that of the control group and DC-T monotherapy group. The expressions of VEGF, IL-6 and IL-17 in tumors were markedly decreased, but IFN-γ and HIF-1α increased after treating with DC-T cells combined with endostatin, compared to control group and DC-T group. In the DCT+endostatin group, the proportions of MDSC and TAM(M2 type) were significantly decreased, m DC and TAM(M1 type) were up-regulated, and CD8+ T cells were recruited to infiltrate tumors, in contrast to PBS control and DC-T monotherapy. DC-T cells combined with endostatin potently reduced the expressions of IL-6, IL-10, TGF-β and IL-17 in tumor tissue, and enhanced the expression of IFN-γ.Conclusions: The study indicated the synergic antitumor effects between endostatin and tumor antigen-pulsed DC-T cells, which may be a prospective therapy strategy to achieve potent antitumor effects on lung cancer. | ring Liang Xiaolin Liu Qi Xie Guoling Chen Xingyu Li Yanrui Jia Beibei Yin Xun Qu Yan Li | 2016 | Chinese Journal of Cancer Research2016，28，4： | 8 |
| 2 | Description of a New Species of the Genus Brachytarsophrys Tian and Hu, 1983(Amphibia: Anura: Megophryidae) from Southern China Based on Molecular and Morphological Data显示文摘A new species, Brachytarsophrys popei sp. nov., is described based on a series of specimens collected from Mount Jinggang, Jiangxi Province, Taoyuandong Nature Reserve, Hunan Province and Nanling Nature Reserve, Guangdong Province, China. The new species can be easily distinguished from other known congeners by morphology, morphometrics and molecular data of the mitochondrial 16 S rRNA gene. It is characterized by its relatively small size with 86.2 mm in snout-vent length in adult female and 70.7 mm–83.5 mm in males; vomerine teeth bearing on two markedly elevated ridges, which projecting behind far beyond the posterior level of the choanae, widely separated by a distance nearly 1.5 times length of one; margin of tongue deeply notched behind; toes about one-third to two-thirds webbed in males, at most one-third webbed in female; the webs extending as a wide fringes along either side of toes; upper eyelid with tubercles, one of which is enlarged and becoming a remarkably prominent, bluntly conical lightyellow horn; black tiny nuptial spines on the dorsal surface of the first finger and second finger base, single vocal sac in males; gravid females bear pure yellowish oocytes; tadpoles with a transverse white stripe on ventral surface and two longitudinal white stripes along the sides of body. The new species represents the fifth known Brachytarsophrys species. | Jian ZHAO Jianhuan YANG Guoling CHEN Chunquan CHEN Yingyong WANG | 2014 | Asian Herpetological Research2014，5，3： | 5 |
| 3 | Loading necrostatin-1 composite bone cement inhibits necroptosis of bone tissue in rabbit显示文摘Bone necrosis after injecting of polymethylmethacrylate(PMMA)bone cement will lead to re-fracture of bone tissue.As a new type of necrosis,there is little research related to the necroptosis of surrounding bone tissue near the bone cement.The purpose of our study was to(i)investigate the presence of necroptosis in vivo and,(ii)established as a new type of bone cement containing PMMA,calcium phosphate cement(CPC)and Necrostatin-1(Nec-1)to inhibit necroptosis of bone tissue.A total of 12 Japanese rabbits were used to establish the animal model and randomly divided into 4 groups signed as a control group,PMMA group,PMMA–CPC group and PMMA–CPC–Nec-1 group,respectively.We used scanning electron microscope to observe the structure of the samples,used HE staining to detect the necrosis,and used western blotting as well as ELISA test to examine the iconic molecule receptor interacting protein kinase-3(RIP 3)protein and tumor necrosis factor a(TNF-a).After analyzing the results of our study,we found that the structure in both PMMA bone cement group and composite bone cement group was damaged and there was an evidence of necrosis,but it was absent in control group.Through molecule detection,the RIP 3 protein expression was decreased in PMMA–CPC–Nec-1(P<0.05).TNF-a expression was increased in bone cement groups with and without CPC(P<0.05),but was inhibited in PMMA–CPC–Nec-1 group.We have concluded that the necroptosis could be confirmed in bone tissue necrosis induced by TNF-a after bone cement injection and also could be inhibited by composite bone cement with Nec-1. | Xiang Ji Feng Xu Guoling Dong Chongzhe Jia Pu Jia Hao Chen Hai Tang | 2019 | Regenerative Biomaterials2019，6，2： | 4 |
| 4 | Fabrication of high-quality all-graphene devices with low contact resistances显示文摘All-graphene 设备是有简单布局和低接触电阻的 graphene 设备的新班。这里,我们为 all-graphene 设备报导干净制造策略经由一帮助缺点的各向异性的蚀刻。同样制作的 graphene 没有污染并且保留太古的 graphene 的质量。在在一条 bilayer graphene 隧道和一个多层的 graphene 电极之间的房间温度(RT ) 的接触抵抗能象 5 | Rong Yang Shuang Wu Duoming Wang Guibai xie Meng Cheng Guole Wang Wei Yang Peng Chen Dongxia Shi Guangyu Zhang | 2014 | Nano Research2014，7，10： | 2 |
| 5 | Development of superconducting microcalorimeters for the HUBS mission显示文摘Hot Universe Baryon Surveyor(HUBS)is a proposed space‐borne observatory for X‐ray astronomy.The primary scientific objectives of the mission are to fill a void in probing the ecosystem of galaxies and thus to advance our understanding of galaxy formation and evolution,which is of fundamental importance in cosmology.More specifically,HUBS aims at directly detecting soft X‐ray emission from diffuse gas of temperature exceeding 106 K,which is theoretically postulated to permeate the large structures in the cosmic web and also fill the extended halo of galaxies.However,although some indirect evidence exists,the presence of such hot gas has yet to be well established observationally,due to the lack of effective tools to probe it.In this paper,we describe the design of HUBS,focusing on its scientific payload,which employs superconducting technologies in the detector system,and particularly on progress in the development of superconducting microcalorimeters. | Sifan Wang Guole Wang Naihui Chen Yanling Chen Wei Cui Jiao Ding Fajun Li Yajie Liang Qian Wang Yeru Wang | 2022 | Superconductivity2022，，4： | 2 |
| 6 | Taxonomy of the White-browed Shortwing(Brachypteryx montana)complex on mainland Asia and Taiwan:an integrative approach supports recognition of three instead of one species显示文摘Background: The White-browed Shortwing(Brachypteryx montana) is widespread from the central Himalayas to the southeast Chinese mainland and the island of Taiwan, the Philippines and Indonesia. Multiple subspecies are recognised, and several of these have recently been suggested to be treated as separate species based on differences in morphology and songs.Methods: We here analyse plumage, morphometrics, songs, two mitochondrial and two nuclear markers, and geographical distributions of the two mainland Asian taxa B. m. cruralis and B. m. sinensis and the Taiwan Residents B. m. goodfellowi.Results: We conclude that these differ congruently in morphology, songs and DNA. Male B. m. goodfellowi is the most divergent in plumage(sexually monomorphic, unlike the two others; male similar to female), and B. m. cruralis and B. m. sinensis differ in male plumage maturation. The song of B. m. cruralis is strongly divergent from the others, whereas the songs of B. m. sinensis and B. m. goodfellowi are more similar to each other. Brachypteryx m. sinensis and B. m. goodfellowi are sisters, with an estimated divergence time 4.1 million years ago(mya; 95% highest posterior distribution [HPD] 2.8–5.5 mya), and B. m. cruralis separated from these two 5.8 mya(95% HPD 4.1–7.5 mya). We also report notable range extensions of B. m. sinensis as well as sympatry between this taxon and B. m. cruralis in Sichuan Province, China. Brachypteryx m. montana from Java is found to be more closely related to Lesser Shortwing(B. leucophris) and Rusty-bellied Shortwing(B. hyperythra) than to the mainland Asian and Taiwan Residents taxa.Conclusion: Our data support a recent proposal to treat the three mainland Asian and Taiwan Residents taxa as three species, separate from B. montana sensu stricto: B. cruralis(central Himalayas to south central China and south Vietnam), B. sinensis(north central to southeastern part of China's Mainland) and B. goodfellowi(Taiwan Island). | Per Alstrom Pamela C.Rasmussen Canwei Xia Magnus Gelang Yang Liu Guoling Chen Min Zhao Yan Hao Chao Zhao Jian Zhao Chengte Yao James A.Eaton Robert Hutchinson Fumin Lei Urban Olsson | 2018 | Avian Research2018，9，4： | 1 |
| 7 | Differential Buck DC/DCChopper Mode Inverters With High -frequency Link 显示文摘 | Daolian Chen Guoling Wang | 2011 | IEEE Trans on Power Electronics2011，26，5： | 1 |
| 8 | A Lifting Method for Fokker-Planck Equations with Drift-Admitting Jumps显示文摘We develop in this paper a lifting method for Fokker-Planck equations with drift-admitting jumps,such that high-order finite difference schemes.can be constructed directly based on grids with pure solution points.To illustrate the idea,we present as an example the construction of a fifth-order finite difference scheme.The validity of the scheme is demonstrated by conducting numerical experiments for the cases with drift admitting one jump and two jumps,respectively.Additionally,by introducing a splitting technique,we show that the lifting method can be extended to high dimensions.In particular,a two-dimensional case is studied in details to show the effectiveness of the extension. | Ningbo Guol Yaming Chen Xiaogang Deng | 2022 | Communications in Mathematical Research2022，38，3： | 1 |
| 9 | High performance work systems and corporate performance: the influence of entrepreneurial orientation and organizational learning显示文摘这研究调查高效工作系统(HPWS ) 怎么影响组织上的性能的工作机制。我们建议(HPWS ) 那能断然通过企业的取向的调停的角色影响组织上的性能。如果它享受组织上的学习的高水平,有高效工作系统的一个组织能更好表现。我们从生产和服务工业设计并且管理并且收到 176 有效回答一张调查问询表到公司的高级行政官或创始人。实验数据的结果显示在高效工作系统和社团的性能之间的关系是更确定的组织上的学习什么时候更强壮。企业的取向部分调停在高效工作系统和组织上的性能之间的关系。这研究由扩大并且合并 HPWS 和企业的取向的解释和预言打开新研究大街,大部分独立于对方被考虑了的二个区域。为实践的含意和为未来研究的方向被提供。 | Chunling Zhu Anqi Liu Guoling Chen | 2018 | Frontiers of Business Research in China2018，12，2： | 1 |
| 10 | Convergence analysis of the preconditioned Gauss-Seidel method for H- matrices显示文摘 | QINGBING LIU GUOLING CHEN JING CAI | 2008 | Computers and Mathematics with Applications2008，56，： | 1 |
| 11 | Differential buck DC- DC chopper mode inverters with high-frequency link显示文摘 | Chen Daolian Wang Guoling | 2011 | IEEE Transactions on Power Electronics2011，26，5： | 1 |
| 12 | enOsCas12f1-mediated exon skipping for Duchenne muscular dystrophy therapy in humanized mouse model显示文摘Duchenne muscular dystrophy(DMD)is a severe neuromuscular disorder caused by mutations in the dystrophin gene,which encodes the essential protein dystrophin.This genetic condition,affecting approximately 1 in 5000 male births worldwide(Birnkrant et al.,2018),is characterized by progressive muscle weakness and wasting,leading to premature death in the second or third decade of life(Farini et al.,2016).The absence of dystrophin protein causes instability in the sarcolemma,which predisposes individuals to myonecrosis and activation of inflammatory signaling cascades.Unfortunately,there is currently no effective cure for DMD,and glucocorticoid steroids are commonly used in clinical settings to delay symptom development(Merlini et al.,2003). | Ming Jin Jiajia Lin Yu Zhang Qingquan Xiao Xiangfeng Kong Xiumei Zhang Zhurui Shao Yin Wang Yuyang Yu Jinjing Li Wan-Jin Chen Guoling Li Hui Yang Ning Wang | 2024 | Journal of Genetics and Genomics2024，51，2： | 0 |
| 13 | Construction of Chaoshan Cuisine Culture Space显示文摘Chaoshan cuisine originates from the unique Chaoshan regional culture and has undergonethousands of years of sedimentation and development. Chaoshan cuisine has a wide range of ingredients,exquisite materials, emphasis on knife cutting, and a focus on freshness. It emphasizes the original taste anda clear and mellow taste, which is extremely characterized by Lingnan culture. In this paper, research dataare collected from social media and online comments, supplemented by targeted interviews, questionnaires,and other methods. It uses grounded theory for data analysis. Through the induction and summary ofopen coding, axial coding, and selective coding based on grounded theory, it summarizes the physical space,cultural space, spiritual cultural space, and communication space of Chaoshan cuisine culture. | CHEN Haiming HUANG Jieli WU Jiayi WANG Guoling LV Canyu WANG Yuping LIU Yifei | 2023 | Journal of Landscape Research2023，15，5： | 0 |
| 14 | Synergistic combination of Pd nanosheets and porous Bi(OH)_(3) boosts activity and durability for ethanol oxidation reaction显示文摘Highly active and durable Pd-based electrocatalysts for ethanol oxidation reaction(EOR)play a crucial role in the commercialization of direct ethanol fuel cells(DEFCs).However,the poisonous intermediates(especially adsorbed CO species(COad))formed during the EOR process can easily adsorb and block the active sites on Pd electrodes,which in turn limits the catalytic efficiency.Hence,we present a series of Pd-based composites with a strong coupling interface consisting of Pd nanosheets and amorphous Bi(OH)_(3)species.The incorporation of Bi(OH)3 can induce an electron-rich state adjacent to the Pd sites and effectively separate the Pd ensemble,leading to excellent CO tolerance.The optimal Pd-Bi(OH)_(3)NSs catalyst manifests a mass activity of 2.2 A·mgPd^(-1),which is 5.7 and 2.0 times higher than that of Pd NSs and commercial Pd/C catalyst,respectively.Further CO-stripping experiments and CO-DRIFTS tests confirm the excellent CO tolerance on Pd-Bi(OH)3 NSs electrode,leading to the enhanced EOR durability. | Mingyu Chu Jialu Huang Jin Gong Yi Qu Guoling Chen Hu Yang Xuchun Wang Qixuan Zhong Chengwei Deng Muhan Cao Jinxing Chen Xiaolei Yuan Qiao Zhang | 2022 | Nano Research2022，15，5： | 0 |
| 15 | DELIBERATlON TO THE DEFINITION OF TOOTH THICKNESS FOR INVOLUTE HELICAL GEAR IN INTERNATIONAL STANDARD ISO1122/1显示文摘DELIBERATlONTOTHEDEFINITIONOFTOOTHTHICKNESSFORINVOLUTEHELICALGEARININTERNATIONALSTANDARDISO1122/1DELIBERATIONTOTHEDEFINITIONO... | Yang Guoling Chen MinLi Xianming Hu Guangxing Feng Shenghua Li Baosong(Shenyang Institute of Gold Technology)( Shenyang Centre of Robot) | 1995 | Chinese Journal of Mechanical Engineering1995，8，3： | 0 |
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