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| 1 | Identification and characterization of microRNAs in raw milk during different periods of lactation, commercial fluid, and powdered milk products显示文摘最近的婴儿的公式奶粉污染事件证明了在牛奶质量控制的经典标记或标准在识别鈥渕a nipulated 鈥 ? 是不够的差质量的牛奶。在现在的学习,我们第一次证明奶牛牛奶包含大量 microRNAs ( miRNAs )并且牛奶特定的 miRNAs 的唯一的表示侧面能为未加工的牛奶和牛奶相关的商业产品的质量控制用作新奇指示物和可能的新标准,例如液体牛奶和粉状的公式牛奶。用定序的 Solexa,首先,我们系统地在未加工的牛奶屏蔽了 miRNA 表示并且在未加工的牛奶识别了 245 miRNAs 的一个总数。不同于层次在哺乳的不同时期是其表达式将近相同的另外的经典 biomarkers,单个 miRNAs 能显著地在哺乳过程期间被改变,含有那 miRNAs 可以是更精确的指示物反映牛奶的优秀改变。第二,使用 TaqMan 基于探查的 miRNA 量的 RT-PCR ,我们进一步识别了七 miRNAs 重要地在整个哺乳过程,和更多有相对一致的表情,这七牛奶特定的 miRNAs 的表示侧面能为区别用作理想的 biomarker 差质量或从纯未加工的牛奶的 鈥渕a nipulated 鈥?牛奶,象为商业乳制品的质量控制一样,例如液体,牛奶和粉状的公式挤。一起,我们的调查结果为为决定未加工的牛奶或牛奶相关的商业产品的质量理解牛奶 miRNAs 和一个新潜在的标准的生理的角色提供一个基础。 | Xi Chen Chao Gao Haijin Li Lei Huang Qi Sun Yanye Dong Chunliang Tian Shengpu Gao Hailin Dong Danping Guan Xiaoyun Hu Shujian Zhao Liang Li Lin Zhu Qiao Yan Junfeng Zhang Ke Zen Chen-Yu Zhang | 2010 | Cell Research2010，20，10： | 29 |
| 2 | Coinfection with influenza A virus enhances SARS-CoV-2 infectivity显示文摘The upcoming flu season in the Northern Hemisphere merging with the current COVID-19 pandemic raises a potentially severe threat to public health.Through experimental coinfection with influenza A virus(IAV)and either pseudotyped or live SARS-CoV-2 virus,we found that IAV preinfection significantly promoted the infectivity of SARS-CoV-2 in a broad range of cell types.Remarkably,in vivo,increased SARS-CoV-2 viral load and more severe lung damage were observed in mice coinfected with IAV.Moreover,such enhancement of SARS-CoV-2 infectivity was not observed with several other respiratory viruses,likely due to a unique feature of IAV to elevate ACE2 expression.This study illustrates that IAV has a unique ability to aggravate SARS-CoV-2 infection,and thus,prevention of IAV infection is of great significance during the COVID-19 pandemic. | Lei Bai Yongliang Zhao Jiazhen Dong Simeng Liang Ming Guo Xinjin Liu Xin Wang Zhixiang Huang Xiaoyi Sun Zhen Zhang Lianghui Dong Qianyun Liu Yucheng Zheng Danping Niu Min Xiang Kun Song Jiajie Ye Wenchao Zheng Zhidong Tang Mingliang Tang Yu Zhou Chao Shen Li Zhou Yu Chen Huan Yan Ke Lan Ke Xu | 2021 | Cell Research2021，31，4： | 16 |
| 3 | Toric Intraocular Lens vs. Peripheral Corneal Relaxing Incisions to Correct Astigmatism in Eyes Undergoing Cataract Surgery显示文摘Purpose:.To compare toric intraocular lens implantation(ToricIOL).with peripheral corneal relaxing incisions(PCRIs) for astigmatism correction in patients undergoing cataract surgery.Methods: 54 patients(54 eyes) with more than 0.75 diopter(D).of preexisting corneal astigmatism were classified as group A(0.75-1.50D) or group B(1.75-2.50D). The patients were randomized to undergo Toric-IOL or PCRIs in the steep axis with spherical IOL implantation..Log MAR uncorrected visual acuity(Log MAR UCVA), Log MAR best corrected vi sual acuity.(Log MAR BCVA),.error of vector(|EV|), surgery induced refraction correction.(|SIRC |),.and correction rates(CR) were measured 1 month and 6 months postoperatively.Results: At 6 months postoperatively, all 54 eyes had LogMAR BCVA≤0.2. Patients who underwent PCRIs and ToricIOL with Log MAR BCVA≤0.1 showed no significant differences in group A(P=1.00) or in group B(P=0.59). Group A showed no significant differences in Log MAR UCVA(P =0.70), |EV|(P=0.13), |SIRC|(P=0.71), and CR(P=0.56)in patients underwent PCRIs and Toric-IOL. However, group B showed significant differences in Log MAR UCVA(P <0.01), |EV|(P<0.01)), |SIRC|(P<0.01), and CR(P<0.01).The Log MAR UCVA and |EV | between 1 and 6 months showed no significant differences in patients in group A. However, in group B, they are significant differences.Conclusion:.The efficacy and stability of Toric-IOL and PCRIs were equal in low astigmatic patients..Toric-IOL achieved an enhanced effect over PCRIs in higher astigmatic patients.PCRIs had the more refractive regression than Toric-IOL in 6months. | Zhiping Liu Xiangyin Sha Xuanwei Liang Zhonghao Wang Jingbo Liu Danping Huang | 2014 | Eye Science2014，29，4： | 9 |
| 4 | Ultraflat, broadband, and highly coherent supercontinuum generation in all-solid microstructured optical fibers with all-normal dispersion显示文摘High flatness, wide bandwidth, and high-coherence properties of supercontinuum(SC) generation in fibers are crucial in many applications. It is challenging to achieve SC spectra in a combination of the properties, since special dispersion profiles are required, especially when pump pulses with duration over 100 fs are employed. We propose an all-solid microstructured fiber composed only of hexagonal glass elements. The optimized fiber possesses an ultraflat all-normal dispersion profile, covering a wide wavelength interval of approximately 1.55 μm. An SC spectrum spanning from approximately 1030 to 2030 nm(corresponding to nearly one octave) with flatness<3 dB is numerically generated in the fiber with 200 fs pump pulses at 1.55 μm. The results indicate that the broadband ultraflat SC sources can be all-fiber and miniaturized due to commercially achievable 200-fs fiber lasers. Moreover, the SC pulses feature high coherence and a single pulse in the time domain, which can be compressed to 13.9-fs pulses with high quality even for simple linear chirp compensation. The Fourier-limited pulse duration of the spectrum is 3.19 fs, corresponding to only 0.62 optical cycles. | CHUNLEI HUANG MEISONG LIAO WANJUN BI XIA LI LILI HU LONG ZHANG LONGFEI WANG GUANSHI QIN TIANFENG XUE DANPING CHEN WEIQING GAO | 2018 | Photonics Research2018，6，6： | 4 |
| 5 | Ocular Surface Characteristics and Impression Cytology in Patients with Active versus Inactive Thyroid Eye Disease显示文摘Purpose:To compare the clinical findings, tear film function and impression cytology between patients with active and inactive Thyroid Eye Disease (TED). Methods:.A total of 56 patients with TED and 30 controls were recruited in this prospective observational cohort study. TED patients were divided into active TED and inactive TED types according to a seven-point modified formulation of the Clinical Activity Score (CAS). All participants underwent full eye examinations including Ocular Surface Disease Index (OSDI) score, tear film break-up time (TBUT), fluorescein staining and Schirmer I test. Thirty nine patients with thyroid-associated orbitopathy.(TAO) received Nelson's grade with conjunctival impression cytology. Proptosis, palpebral fissure width and lagophthalmos were assessed. Results:.Ocular surface parameters including proptosis, palpebral fissure width and lagophthalmos did not differ between active and inactive TED patients(P>0.05). Both active and inactive TED patients obtained higher fluorescein staining scores, lower TBUT scores and significantly lower Schirmer test scores than those of controls (P<0.001 for all). Additionally, the TBUT score was significantly lower and the OSDI score significantly higher in the active TED group compared with those in the inactive TED group (P<0.001 for both). Impression cytology revealed a higher proportion of grade 2-3 changes in the active TED group compared with the inactive TED group(P<0.001). Conclusion:.Orbital inflammation in TED patients may lead to decreased tear film stability and ocular surface squamous metaplasia. | Nuo Xu Danping Huang Huasheng Yang Zhaoguang Lai Quan Luo | 2012 | Eye Science2012，27，2： | 3 |
| 6 | The application of label-free imaging technologies in transdermal research for deeper mechanism revealing显示文摘The penetration behavior of topical substances in the skin not only relates to the transdermal delivery efficiency but also involves the safety and therapeutic effect of topical products,such as sunscreen and hair growth products.Researchers have tried to illustrate the transdermal process with diversified theories and technologies.Directly observing the distribution of topical substances on skin by characteristic imaging is the most convincing approach.Unfortunately,fluorescence labeling imaging,which is commonly used in biochemical research,is limited for transdermal research for most topical substances with a molecular mass less than 500 Da.Label-free imaging technologies possess the advantages of not requiring any macromolecular dyes,no tissue destruction and an extensive substance detection capability,which has enabled rapid development of such technologies in recent years and their introduction to biological tissue analysis,such as skin samples.Through the specific identification of topical substances and endogenous tissue components,label-free imaging technologies can provide abundant tissue distribution information,enrich theoretical and practical guidance for transdermal drug delivery systems.In this review,we expound the mechanisms and applications of the most popular label-free imaging technologies in transdermal research at present,compare their advantages and disadvantages,and forecast development prospects. | Danping Zhang Qiong Bian Yi Zhou Qiaoling Huang Jianqing Gao | 2021 | Asian Journal of Pharmaceutical Sciences2021，16，3： | 2 |
| 7 | Vasorelaxant effect of osthole on isolated thoracic aortic rings in rats显示文摘OBJECTIVE: To investigate the effect of osthole on isolated thoracic aortic rings, and to determine the potential mechanism of action.METHODS: Thoracic aortas were isolated from Wistar rats, and were suspended in tissue organ chambers for vascular tension measurement. The effect of cumulative osthole(10-9, 10-8, 10-7, 10-6, and 10-5 mol/L) on endothelium-intact and endothelium-denuded thoracic aortic rings pre-contracted with phenylephrine(PE, 10-6 mol/L) or KCl(6 × 10-2 mol/L) was recorded. Histomorphological changes of thoracic aorta were analyzed by hematoxylin-eosin. The effects of different osthole concentrations on endothelium-intact aortic rings, which were pre-inhibited with the non-selective nitric oxide synthase inhibitor L-Arg(NO2)-OMe·HCl(3 × 10-4 mol/L), endothelium-derived nitric oxide synthase inhibitor Nω-nitro-L-arginine(3 × 10-4 mol/L), guanylate cyclase inhibitor 1 H-[1,2,4] oxadiazolo [4,3-α]quinoxaline-1-one(10-5 mol/L), cyclooxygenase inhibitor indometacin(10-5 mol/L), and the Ca2+-activated potassium channel inhibitor tetraethylammonium nitrate(10-5 mol/L), and then contracted with PE, were examined. Aortic rings incubated with osthole(10-5 mol/L), phentolamine(10-5 mol/L), or verapamil(10-5 mol/L) in Ca2+-free Krebs-Henseleit solution(KHS) were stimulated with PE or KCl.RESULTS: There was a dose-dependent increase in vasorelaxation of isolated thoracic aortic rings(both with and without endothelium) with increasing osthole concentration. Hematoxylin-eosin staining showed that osthole significantly improved thoracic aorta ring morphology. Compared with the control group, there were also significant differences after incubation with L-Arg(NO2)-OMe ω-nitro-L-arginine, and 1 H-[1,2,4] oxadiazo·lo HCl,N [4,3-α] quinoxaline-1-one(P < 0.05 for all). The relaxation rate of the rings in the osthole group incubated with indometacin and tetraethylammonium nitrate were similar to controls. In Ca2+-free KHS, the PE-induced contraction was similar between the osthole(4.37% ± 0.41%) and control(4.21% ± 1.33%)groups. However, after cumulative CaCl2(0.5, 1, 1.5,2, 2.5, and 3 mmol/L), the Ca2+-induced contraction was significantly inhibited in the osthole and phentolamine groups compared with controls(P < 0.05).After cumulative CaCl2 was added to Ca2+-free KHS(high K+ concentration), the contraction rate was significantly higher than both of the control and the osthole groups(P < 0.05). The contraction rate in the osthole group was higher than the verapamil group(P < 0.05).CONCLUSION: Osthole has a vasorelaxant effect on isolated rat thoracic aortic rings, via inhibition of both receptor-operated and voltage-dependent Ca2+channels in arterial smooth muscle, leading to decreased Ca2+ influx, and via inhibition of nitric oxide release on arterial endothelial cells. | Huang Ying Kong Liting Wang Yanping Sun Mingjie Cui Haifeng Zhai Huaqiang LüCheng Fan Danping | 2019 | Journal of Traditional Chinese Medicine2019，39，4： | 1 |
| 8 | Inhibition on Telomerase Activity and Cytotoxic Effects by Cisplatin in Cultured Human Choroidal Melanoma Cells显示文摘Purpose: To study the changes of telomerase activity and cytotoxic effects by Cisplatin;cis-dichlorodiamine platinum (CDDP) in cultured human choroidal melanoma.Material and Methods: The primary cultured human choroidal melanoma cells werecultured in the presence and absence of CDDP with different concentration and timerespectively. The toxic effects were evaluated by MTT and the level of telormarse wasdetected by PCR-ELISA assay. And the relationship between telomerase activity andcytotoxic effects were analyzed by a correlation analysis.Results: Following the increase of the concentration and the time of CDDP, graduallyrepressed telomerase activity was detected in cultured cells. Meanwhile, the restrain rateof the cells increased. The telomerase activity at 24h and 1μg/ml was repressedsignificantly compared with the control cells. However, the appearance of cell deathlagged behind the decreasing of telomerase.Conclusions: CDDP is an effective telomerase inhibitor in cultured choroidal melanomacells of human eyes, which presents concentration and time dependency and can causethe death of cultured cells. | Hao Cheng, Zhongyao Wu, Jianliang Zheng, Guilan Lu, Jianhua Yan Min Liu, Danping Huang, Jianxian LinZhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou 510060, China | 2003 | Eye Science2003，19，1： | 1 |
| 9 | Maternal butyrate supplementation affects the lipid metabolism and fatty acid composition in the skeletal muscle of offspring piglets显示文摘Maternal sodium butyrate(SB)intake has important effects on offspring growth and development.This study aimed to investigate the impacts of maternal SB supplementation during gestation and lactation on fatty acid composition and lipid metabolism in the offspring skeletal muscle of pigs.Twenty sows(Yorkshire,parity 2 to 3)were assigned to the control group(diets without SB,n=10)and SB group(diets with 0.1%SB,n=10).The results showed maternal SB supplementation throughout gestation and lactation increased(P<0.05)body weight of offspring piglets at weaning.The concentrations of triglyceride in plasma and milk were enhanced(P<0.05).Maternal SB induced(P<0.05)lipid accumulation with increased expression of peroxisome proliferator activated receptorγ(PPARγ)by enrichment of the acetylation of H3 acetylation K27(H3 K27)in offspring skeletal muscle.Meanwhile,the concentrations of C18:2 n-6,C18:3 n-3,total polyunsaturated fatty acid(PUFA),n-6 PUFA and n-3 PUFA decreased(P<0.05)in skeletal muscle of weaning piglets derived from SB sows.Together,these results showed that maternal SB supplementation could influence offspring growth performance,lipid metabolism and fatty acid composition of the skeletal muscle. | Yongsen Zhao Danping Wang Yanping Huang Dangdang Wu Xiaoming Ji Xiaobing Zhou Dong Xia Xiaojing Yang | 2021 | Animal Nutrition2021，，4： | 1 |
| 10 | A Modified Evisceration Technique with Scleral Quadrisection and Porous Polyethylene Implantation显示文摘 | Danping Huang Yangyang Yu Rong Lu Huasheng Yang Jianhao Cai | 2009 | American Journal of Ophthalmology2009，，5： | 1 |
| 11 | The effect of lengths of branched-chain fatty alcohols on the efficacy and safety of docetaxel-prodrug nanoassemblies显示文摘The self-assembly prodrugs are usually consisted of drug modules,activation modules,and assembly modules.Keeping the balance between efficacy and safety by selecting suitable modules remains a challenge for developing prodrug nanoassemblies.This study designed four docetaxel(DTX)prodrugs using disulfide bonds as activation modules and different lengths of branched-chain fatty alcohols as assembly modules(C_(16),C_(18),C_(20),and C_(24)).The lengths of the assembly modules determined the self-assembly ability of prodrugs and affected the activation modules’sensitivity.The extension of the carbon chains improved the prodrugs’self-assembly ability and pharmacokinetic behavior while reducing the cytotoxicity and increased cumulative toxicity.The use of C_(20) can balance efficacy and safety.These results provide a great reference for the rational design of prodrug nanoassemblies. | Shuo Wang Tian Liu Yuetong Huang Chaoying Du Danping Wang Xiyan Wang Qingzhi Lv Zhonggui He Yinglei Zhai Bingjun Sun Jin Sun | 2024 | Acta Pharmaceutica Sinica B2024，14，3： | 0 |
| 12 | Recommendation on data collection and annotation of ocular appearance images in ptosis显示文摘Ptosis is a common ophthalmologic condition,and the diagnosis is primarily based on ocular appearance.Thediagnosis of such conditions can be improved using emerging technology such as artificial intelligence-basedmethods.However,unified data collection and labeling standards have not yet been established.This directlyimpacts the accuracy of ptosis diagnosis based on appearance alone.Therefore,in the present study,we aimedto establish a procedure to obtain and label images to devise a recommendation system for optimal recognitionof ptosis based on ocular appearances.This would help to standardize and facilitate data sharing and serve as aguideline for the development and improvisation of algorithms in artificial intelligence for ptosis. | Jie Meng Binying Lin Dongmei Li Shiqi Hui Xuanwei Liang Xianchai Lin Zhen Mao Xingyi Li Zuohong Li Rongxin Chen Yahan Yang Ruiyang Li Anqi Yan Haotian Lin Danping Huang Chinese Association of Artificial Intelligence,Medical Artificial IntelligenceBranch of Guangdong Medical Association | 2023 | Intelligent Medicine2023，3，4： | 0 |
| 13 | Effectiveness and safety of red yeast rice predominated by monacolin Kβ-hydroxy acid form for hyperlipidemia treatment and management显示文摘OBJECTIVE:To assess the lipid-lowering activity and safety of a dietary supplement containing monacolin Kβ-hydroxy acid form(MKA),Heye(Folium Nelumbinis),and Cangzhu(Rhizoma Atractylodis Lanceae),compared to lifestyle modifications.METHODS:Totally 117 subjects with moderate to severe dyslipidemia(according to Chinese guidelines)and low CV risk were randomly assigned into three treatment groups:lifestyle modification(LM),LM plus a low dosage of MKA,LM plus a high dosage of MKA,and treated for 60 d.The primary endpoint was the reduction of low density lipoprotein cholesterol(LDL-C)and total cholesterol(TC).Safeties along with Traditional Chinese Medicine Syndromes were assessed through the study.RESULTS:A low dosage of MKA along with lifestyle modifications caused a significant decrease in LDL-C by 15.6%on average(95%CI,9.6%to 21%)with,a decrease in TC by 15.3%on average(95%CI,9.26%to 21.4%),and a decrease in non-HDL-C by 35.4%(95%CI,25.76%to 41.34%).Weak evidence of a reduction of triglycerides but an increment of HDL-C was observed in patients with severe hyperlipidemia.No severe adverse events occurred during the study.CONCLUSION:Our results confirm the LDL-C and TC lowering properties of MKA is clinically meaningful.It also produces a significant reduction of non-HDL-C,and slightly effects on TG and HDL-C as well. | CHEN Benjian HUANG Xiaodan PENG Huiting LI Yishi CAO Yongtao WU Huanlin XU Danping | 2022 | Journal of Traditional Chinese Medicine2022，42，2： | 0 |
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