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| 1 | Effects of different biochars on antibiotic resistance genes during swine manure thermophilic composting显示文摘Elimination of antibiotic resistance genes(ARGs)in animal manure from concentrated animal feeding operations by thermophilic composting has drawn increasing attention.This study investigated the effects of sawdust biochar,corn stover biochar and peanut hull biochar with three spiked levels on ARGs in swine manure during thermophilic composting.Thirteen ARGs corresponding to four classes of antibiotics(tetracyclines,sulfonamides,macrolides and quinolones)were determined in the composting piles.Results indicated that the ten tested composting groups became fully mature after 30-day thermophilic composting process.tetM,tetO and ermB were reduced in all tested groups;tetC and tetG,tetX,sul1,sul2,ermF,qnrD and aac(6’)-Ib were mostly reduced under low level biochar addition but increased under higher level biochar addition;gyrA increased under medium biochar addition and reduced in other groups;oqxB remained comparatively stable throughout the composting process.The addition levels of spiked biochar are more important than types of spiked biochar on the removal of ARGs in the composting pile.The average removal rates of ARGs in the control group,low,medium and high level biochar addition groups were 0.24 logs,0.52-0.72 logs,-0.52-0.18 logs and-0.19-0.21 logs,respectively.In summary,low level biochar addition could enhance the elimination of studied ARGs in swine manure during the composting process,while medium level biochar addition to the composting piles would increase the risk of ARGs’propagation. | Jian Wang Bin Sui Yujun Shen Haibo Meng Lixin Zhao Haibin Zhou Ran Li Jingtao Ding Hongsheng Cheng Xi Zhang | 2018 | International Journal of Agricultural and Biological Engineering2018，11，6： | 5 |
| 2 | Adenosine monophosphate-activated protein kinase activation enhances embryonic neural stem cell apoptosis in a mouse model of amyotrophic lateral sclerosis显示文摘Alterations in embryonic neural stem cells play crucial roles in the pathogenesis of amyotrophic lateral sclerosis. We hypothesized that embryonic neural stem cells from SOD1G93 A individuals might be more susceptible to oxidative injury, resulting in a propensity for neurodegeneration at later stages. In this study, embryonic neural stem cells obtained from human superoxide dismutase 1 mutant(SOD1G93A) and wild-type(SOD1WT) mouse models were exposed to H2O2. We assayed cell viability with mitochondrial succinic dehydrogenase colorimetric reagent, and measured cell apoptosis by flow cytometry. Moreover, we evaluated the expression of the adenosine monophosphate-activated protein kinase(AMPK) α-subunit, paired box 3(Pax3) protein, and p53 in western blot analyses. Compared with SOD1 WT cells, SOD1G93 A embryonic neural stem cells were more likely to undergo H2O2-induced apoptosis. Phosphorylation of AMPKα in SOD1G93 A cells was higher than that in SOD1 WT cells. Pax3 expression was inversely correlated with the phosphorylation levels of AMPKα. p53 protein levels were also correlated with AMPKαphosphorylation levels. Compound C, an inhibitor of AMPKα, attenuated the effects of H2O2. These results suggest that embryonic neural stem cells from SOD1G93 A mice are more susceptible to apoptosis in the presence of oxidative stress compared with those from wild-type controls, and the effects are mainly mediated by Pax3 and p53 in the AMPKα pathway. | Yanling Sui Zichun Zhao Rong Liu Bin Cai Dongsheng Fan | 2014 | Neural Regeneration Research2014，9，19： | 3 |
| 3 | Investigation and analysis of the linkage mechanism and whole process cost of livestock manure organic fertilizer显示文摘In recent years,the utilization of manure resources for livestock and poultry farming has attracted a widespread attention,and manure resources utilization models suitable for different regional characteristics have formed gradually.Among them,the production of organic fertilizer from animal manure is a vital utilization method.However,there are still some problems such as high production costs,difficult sales,and the unwillingness of farmers to use organic fertilizers which have affected the breeding cycle and the sustainability of manure treatment in livestock and poultry breeding.This article selected 371 organic fertilizer plants,related farms and farmers in China,focusing on the main links of the entire process of livestock manure-organic fertilizer-farm application,and studied the mode of animal manure collection by organic fertilizer plants.The costs of organic fertilizer production and farmland application were discussed.Moreover,suggestions were made for the promotion and implementation of large-scale organic fertilizers to make good utilization of manure resources in livestock and poultry farming. | Shanshan Dong Bin Sui Yujun Shen Haibo Meng Lixin Zhao Jingtao Ding Haibin Zhou Xi Zhang Hongsheng Cheng Jian Wang Ran Li Liqiu Song Pengxiang Xu Ji Li | 2020 | International Journal of Agricultural and Biological Engineering2020，13，2： | 2 |
| 4 | Diamond wheel grinding characteristics of 3D orthogonal quartz fiber reinforced silica ceramic matrix composite显示文摘Fiber-reinforced silica ceramic matrix composites(SiO_(2) f/SiO_(2))have gained extensive attention in recent years for its applications in aeronautics field such as radar radome and window.However,the machining properties and mechanism of the material remain unclear.The features and mechanical properties of the material itself have a significant influence on both its machining characteristics and surface integrity.Thus,a full-factor grinding experiment is conducted using a 3 D orthogonal SiO_(2) f/SiO_(2) aiming to obtain its machining characteristics.The effects of grinding parameters and tools on the grinding force,surface roughness,and material damage type are investigated using a dynamometer,Scanning Electron Microscope(SEM),and Acoustic Emission(AE)analysis.The AE frequency band is analyzed,and a semi-analytical force model is established to study the difference between a single grain and wheel grinding.It was found that the changes in surface roughness correlate with the changes in grinding force,with fiber fracture being the main reason behind the increase in grinding force.Finally,the material removal mechanism was studied based on the AE analysis.It was found that the removal mechanism is fiber fracture dominated with matrix crack and debonding,and the primary sources of energy consumption are fiber fracture and friction. | Bin LIN Haoji WANG Jinhua WEI Tianyi SUI | 2021 | Chinese Journal of Aeronautics2021，34，5： | 2 |
| 5 | PCR-based approaches for identification of multi-copy transgene integration sites in mouse genome显示文摘Generation of transgenic mice by DNA microinjection has become one of the most important technologies in studying gene function in vivo. Ran- dom integration of transgene often results in inser- tional mutation which may complicate phenotype analysis of transgenic mice and/or create a good opportunity to study the function of endogenous gene. However,the utilization of these potentially valuable resources is hampered due to lack of efficient ap- proaches for rapid identification of multi-copy trans- gene integration sites. Here we propose two PCR-based approaches to identifying trans- gene/genome junction sequences. The efficiency of these two strategies was tested in 9 independent transgenic mouse lines. Among them,the transgene in 8 mouse lines was clearly localized to certain chromosome regions. These rapid and efficient ap- proaches will greatly facilitate the study of insertional mutation due to transgene integration. | ZHAO Xudong DANG Suying LIANG Bin LEI Xia CHEN Zheng WANG Long YAN Lanzhen SUN Hantang FU Jiliang FEI Jian WANG Zhugang | 2006 | Chinese Science Bulletin2006，51，18： | 2 |
| 6 | Ultralight aerogel sphere composed of nanocellulose-derived carbon nanofiber and graphene for excellent electromagnetic wave absorption显示文摘A novel type of three-dimensional ultralight aerogel sphere,consisting of one-dimensional nanocellulose-derived carbon fibers and two-dimensional graphene layers,was prepared based on a developed drop-freeze-drying followed by carbonization approach.The nanofibrous carbon efficiently prevents the agglomeration of the graphene layers,which,in turn,reduces the shrinkage and maintains the structural stability of the hybrid carbon aerogel spheres.Consequently,the aerogel spheres showing an ultralow-density of 2.8 mg/cm^(3) and a porosity of 99.98%accomplish the tunable dielectric property and electromagnetic wave(EMW)absorption performance.The high-efficiency utilization of biomass-derived fibrous nanocarbon,graphene,the porous structure of the hybrid aerogel spheres leads to the excellent EMW absorption performance.The aerogel spheres display an effective absorption bandwidth of 6.16 GHz and a minimum reflection loss of−70.44 dB even at a filler loading of merely 3 wt.%,significantly outperforming that of other biomass-derived carbon-based EMW absorbing materials.This work offers a feasible,facile,scalable approach for fabricating high-performance and sustainable biomass-based aerogels,suggesting a tremendous application potential in EMW absorption and aerospace. | Runa Zhang Bin Li Yunfei Yang Na Wu Zhuyin Sui Qingfu Ban Lili Wu Wei Liu Jiurong Liu Zhihui Zeng | 2023 | Nano Research2023，16，5： | 2 |
| 7 | Antisense expression of PKCα improved sensitivity of SGC7901/VCR cells to doxorubicin显示文摘AIM:To explore whether antisense blocking of protein kinase C alpha(PKCα)would reverse multi-drug resistance(MDR)in the vincristine(VCR)-resistant human gastric cancer cell line SGC7901/VCR. METHODS:SGC7901/VCR cells expressing antisense PKCα,SGC7901/VCR/aPKC,were established by transfection with a recombinant plasmid reversely inserted with PKCαcDNA.Empty vector(PCI-neo)transfected cell clones,SGC7901/VCR/neo,served as the control.Western blot method was used to detect PKCαcontent in SGC7901,SGC7901/VCR,SGC7901/ VCR/neo and SGC7901/VCR/aPKC cells,using PKCα-specific antibody.The sensitivity of SGC7901,SGC7901/ VCR,SGC7901/VCR/neo and SGC7901/VCR/aPKC cells to doxorubicin(DOX)in vitro was determined by MTT assay.The uptake of DOX in these cells was detected with fluorescence spectrophotometer.RESULTS:Western blot analysis showed that the PKCαprotein level was about 8.7-fold higher in SGC7901/ VCR cells than that in SGC7901 cells,whereas the protein expression of PKCαwas reduced by 78%in SGC7901/VCR/aPKC cells when compared with the SGC7901/VCR cells.SGC7901/VCR/aPKC cells had a 4.2-fold increase in DOX cytotoxicity,accompanied by a 1.7-fold increase of DOX accumulation in comparison with SGC7901/VCR cells. CONCLUSION:PKCαpositively regulates MDR in SGC7901 cells,and inhibition of PKCαcan partially attenuate MDR in human gastric cancer cells. | Da-Long Wu Feng-Ying Sui Cheng Du Cheng-Wen Zhang Bin Hui Shui-Ling Xu Huan-Zhang Lu Guo-Jie Song | 2009 | World Journal of Gastroenterology2009，15，10： | 2 |
| 8 | A haplotype map of genomic variations and genome-wide association studies of agronomic traits in foxtail millet(Setaria italica)显示文摘 | Guanqing Jia Xuehui Huang Hui Zhi Yan Zhao QiangZhao Wenjun Li Yang Chai Lifang Yang Kunyan Liu Hengyun Lu Chuanrang Zhu Yiqi Lu Congcong Zhou Danlin Fan Qijun Weng Yunli Guo Tao Huang LeiZhang Tingting Lu Qi Feng Hangfei Hao HongkuanLiu Ping Lu Ning Zhang Yuhui Li Erhu Guo ShujunWang Suying Wang Jinrong Liu Wenfei Zhang GuoqiuChen Baojin Zhang Wei Li Yongfang Wang HaiquanLi Baohua Zhao Jiayang Li Xianmin Diao Bin HanJia | 2013 | Nature Genetics2013，45，8： | 1 |
| 9 | Revealing the residual stress distribution in laser welded Eurofer97 steel by neutron diffraction and Bragg edge imaging显示文摘Eurofer97 steel is a primary structural material for applications in fusion reactors. Laser welding is a promising technique to join Eurofer97 plasma-facing components and overcome remote handling and maintenance challenges. The interaction of the induced residual stress and the heterogeneous microstructure degrades the mechanical performance of such fusion components. The present study investigates the distribution of residual stress in as-welded and post-heat treated Eurofer97 joints. The mechanistic connections between microstructure, material properties, and residual stress are also studied. Neutron diffraction is used to study the through-thickness residual stress distribution in three directions,and neutron Bragg edge imaging(NBEI) is applied to study the residual strain in high spatial resolution.The microstructures and micro-hardness are characterised by electron backscatter diffraction and nanoindentation, respectively. The M-shaped residual stress distribution through the thickness of the as-welded weldment is observed by neutron diffraction line scans over a region of 1.41 × 10 mm^(2). These profiles are cross-validated over a larger area(∼56 × 40 mm^(2)) with the higher spatial resolution by NBEI. The micro-hardness value in the fusion zone of the as-welded sample almost doubles from 2.75 ± 0.09 GPa to 5.06 ± 0.29 GPa due to a combination of residual stress and cooling-induced martensite. Conventional post weld heat treatment(PWHT) is shown to release ∼90% of the residual stress but not fully restore the microstructure. By comparing its hardness with that of stress-free samples, it is found that the microstructure is the primary contribution to the hardening. This study provides insight into the prediction of structural integrity for critical structural components of fusion reactors. | Bin Zhu Nathanael Leung Winfried Kockelmann Saurabh Kabra Andrew JLondon Michael Gorley Mark JWhiting Yiqiang Wang Tan Sui | 2022 | Journal of Materials Science & Technology2022，，19： | 1 |
| 10 | Influence of particle size and ionic strength on the freeze-thaw stability of emulsions stabilized by whey protein isolate显示文摘The influence of particle size and ionic strength on the freeze-thaw(FT) stability of emulsions stabilized by whey protein isolate(WPI) was investigated in this study. The destabilization of emulsions during the FT process could be suppressed in a way by decreasing the particle size of the initial emulsions, which was the result of retarding the coalescence between oil droplets. To further improve the FT stability of emulsions, different amounts of Na Cl were added before emulsification. The emulsions with the ionic strength at 30–50 mmol/L exhibited good FT stability. Notably, the ionic strength in this range would not lower the freezing point of emulsions below the freezing temperature used in this study. Salt addition could improve the structural properties of proteins, which was available to strengthen the rigidity and thickness of interfacial layers, sequentially building up the resistance that the destruction of ice crystals to emulsions. Moreover, stronger flocculation between emulsion droplets could promote the formation of a gel-like network structure dominated by elasticity in the emulsion system, which might effectively inhibit the movement of droplets, and improve the FT stability of emulsions eventually. The result was of great significance for the preparation of emulsion-based foods with improved FT stability. | Hao Lai Fuchao Zhan Yujie Wei Abel W.S.Zongo Sha Jiang Haomin Sui Bin Li Jing Li | 2022 | Food Science and Human Wellness2022，11，4： | 1 |
| 11 | Immune characterization of mesenchymal stem cells in human umbilical cord Wharton’s jelly and derived cartilage cells显示文摘 | Shuyun Liu Mei Yuan Kedong Hou Li Zhang Xifu Zheng Bin Zhao Xiang Sui Wenjing Xu Shibi Lu Quanyi Guo | 2012 | Cellular Immunology (-)2012，，1： | 1 |
| 12 | What is the choice for supercapacitors: graphene or graphene oxide显示文摘 | Xu Bin Yue Shu-fang Sui Zhu-yin | 2011 | Energy & Environmental Science2011，4，8： | 1 |
| 13 | MCM 7 expression predicts post‐operative prognosis for hepatocellular carcinoma显示文摘 | Yan‐Ming Zhou Xiao‐Feng Zhang Lu Cao Bin Li Cheng‐Jun Sui Yu‐Min Li Zheng‐Feng Yin | 2012 | Liver Int2012，，10： | 1 |
| 14 | 华南地区煅烧粘土-石灰石复合水泥(LC^3)混凝土的研发及其与钢筋的粘结性能显示文摘目的:采用华南地区的原材料研发一种LC3水泥胶凝材料,并研究其水化反应、微观结构的发展、工作性能、强度性能的发展以及混凝土和钢筋之间的粘结-滑移行为,为推广LC3水泥胶凝材料应用于滨海结构混凝土提供基础信息和参考。创新点:1.鉴于目前对LC3水泥胶凝材料在混凝土技术和结构混凝土中的应用研究较少,本文对采用华南地区原材料制备的LC3混凝土进行了技术研究和应用分析;2.试验结果发现,LC3混凝土的抗折和劈裂性能优于相同抗压强度的普通混凝土;3.采用拔出试验研究LC3混凝土与钢筋之间的界面粘结-滑移行为,证明了新型LC3水泥胶凝材料在钢筋混凝土结构中的适用性。方法:1.开展一系列基于LC3水泥的净浆、砂浆和混凝土的实验研究,并使用原始材料进行微观结构分析(水化产物表征和孔结构分析)和宏观测试(工作性能和力学性能测试)。2.研究LC3混凝土与钢筋之间的粘结-滑移行为。结论:1.与普通混凝土(OPC)相比,钙矾石、单碳铝酸盐(MC)和半碳铝酸盐(HC)是LC3样品中的主要晶体水合产物;由于熟料减少50%,且LC3中发生的二次水化反应消耗了大量OPC熟料水化生成的氢氧化钙(CH),因此在LC3的水合产物中几乎没有残留六角形薄片CH。2.与OPC砂浆和混凝土相比,LC3砂浆和混凝土的早期强度较低;由于在煅烧粘土中无定形二氧化硅和氧化铝(与石灰石结合)二次反应形成了更多的水化硅酸钙和碳铝酸钙水合物,LC3砂浆和混凝土的后期强度(7天后)迅速增加至与OPC砂浆和混凝土相当的强度水平。3.在与OPC同类材料具有相同抗压强度的情况下,LC3水泥胶凝材料具有更高的抗折和劈裂强度,因此更有韧性。4.对于LC3水泥胶凝材料,颗粒更细的石灰石不一定会带来更好的力学性能。5.对于混凝土与钢筋之间的界面粘结-滑移行为,LC3的破坏模式与OPC大致相同,且通常有两种破坏模式,即钢筋从混凝土中拔出和混凝土劈裂破坏;在具有相同等级的抗压强度时,LC3混凝土的粘结强度与OPC混凝土相当,但其粘结-滑移刚度更大。 | Zhen-yu HUANG You-shuo HUANG Wen-yu LIAO Ning-xu HAN Ying-wu ZHOU Feng XING Tong-bo SUI Bin WANG Hong-yan MA | 2020 | Journal of Zhejiang University-Science A(Applied Physics & Engineering)2020，21，11： | 1 |
| 15 | Preparation and magnetic properties of anisotropic Nd2Fe14B/Sm2Co17 hybrid-bonded magnets显示文摘In the present work, anisotropic Nd2 Fe14 B/Sm2 Co17 hybrid-bonded magnets were prepared with different Nd-Fe-B contents. It is found that the particle distributions and ratios between the two magnetic phases have important roles in the magnetic properties, microstructures and thermal stability of the magnets. With increase of Nd-Fe-B content, the saturation magnetization of the anisotropic hybrid magnet increases significantly, however, coercivity decreases, and the demagnetization curves show magnetically single-phase behavior. The anisotropic Nd2 Fe14 B/Sm2 Co17 hybrid-bonded magnets exhibit a maximum energy product and remanence of 14.15 MGOe and 99.53 A·m2/kg, respectively, when the NdFe-B content is 70 wt% at room temperature. Furthermore, the hybrid magnets also have better thermal stability at elevated temperatures due to the interaction between the two magnetic particles. | Xueguo Yin Yue Sui Qiqi Yang Qian Shen Yilong Ma Bin Shao Jianchun Sun Donglin Guo | 2019 | Journal of Rare Earths2019，37，10： | 1 |
| 16 | Probabilistic characterization and experimental study for variation of load spectrum in a fleet显示文摘 | He Xiaofan Sui Fucheng Zhai Bin Liu Wenting | 2013 | Engineering Failure Analysis2013，33，41： | 1 |
| 17 | An improved water-use efficiency for winter wheat grown under reduced irrigation显示文摘 | Jianhua Zhang Xiangzhen Sui Bin Lin | 1998 | Field Crop Research1998，59，： | 1 |
| 18 | Mechanically strong and highly conductive graphene aerogel and its use as electrodes for electrochemical power sources显示文摘 | ZHANG Xuetong SUI Zhuyin XU Bin | | 0，，： | 1 |
| 19 | IABC: An iteratively automatic machine learning boosted hand-eye calibration method for laser displacement sensor显示文摘An on-machine measuring(OMM)system with a laser displacement sensor(LDS)is designed for measuring free-form surfaces of hypersonic aircraft’s radomes.To improve the measurement accuracy of the OMM system,a novel Iteratively Automatic machine learning Boosted hand-eye Calibration(IABC)method is proposed.Both the hand-eye relationship and LDS measurement errors can be calibrated in one calibration process without any hardware changes via IABC.Firstly,a new objective function is derived,containing analytical parameters of the handeye relationship and LDS errors.Then,a hybrid calibration model composed of two kernels is proposed to solve the objective function.One kernel is the analytical kernel designed for solving analytical parameters.Another kernel is the automatic machine learning(AutoML)kernel designed to model LDS errors.The two kernels are connected with stepwise iterations to find the best calibration results.Compared with traditional methods,hand-eye experiments show that IABC reduces the calibration RMSE by about 50%.Verification experiments show that IABC reduces the measurement deviations by about 25%-50%and RMSEs within 40%.Even when the training data are obviously less than the test data,IABC performs well.Experiments demonstrate that IABC is more accurate than traditional hand-eye methods. | Helin LI Bin LIN Tianyi SUI Liang XU Chen ZHANG Shuai YAN Yang WANG Xinquan HAO Deyu LOU | 2023 | Chinese Journal of Aeronautics2023，36，8： | 0 |
| 20 | Carbon fiber cannot always reduce the wear of PEEK for orthopedic implants under DPPC lubrication显示文摘Excellent wear resistance is an important feature of orthopedic implants.However,although pure polyetheretherketone(PEEK)is outperformed by carbon fiber-reinforced PEEK(CF-PEEK)for stability and durability under laboratory conditions,it is not clear whether CF-PEEK should be preferred in all real-world applications.Results indicate that,under dipalmitoylphosphatidylcholine(DPPC)lubrication,the wear rates of PEEK are 35%-80%lower than the wear rates of CF-PEEK for different implant materials,speeds,loadings,and DPPC concentrations.Molecular dynamics calculations confirm that DPPC self-assembles on the PEEK surface to form an easily adsorbed continuous phospholipid lubricating film.In contrast,the carbon fibers on the CF-PEEK surface hinder the formation of the protective DPPC film and the CF-PEEK surface is thus subject to faster wear. | Shuai YAN Shichao MEN Hongbo ZOU Haoji WANG Zhongjiang ZHANG Chunshen WANG Tianyi SUI Bin LIN | 2023 | Friction2023，11，3： | 0 |
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