Role of neurotrophic factors in enhancing linear axonal growth of ganglionic sensory neurons in vitro

查看全文 作　　者：Michele [1]Fornaro;Alessia [2]Giovannelli;Angelica [3]Foggetti;Luisa [2,4]Muratori;Stefano [2,4]Geuna;Giorgia [5]Novajra;Isabelle [2,4]Perroteau

机构地区：[1]Department of Anatomy,College of Graduates Studies(CGS),Chicago College of Osteopathic Medicine(CCOM),Midwestern University,Downers Grove,IL,USA;[2]Department of Clinical and Biological Sciences,University of Turin,Torino,Italy;[3]Institute of Physiology,Christian-Albrechts-University Kiel,Kiel,Germany;[4]Neuroscience Institute Cavalieri Ottolenghi(NICO),Torino,Italy;[5]Department of Applied Science and Technology,Politecnico di Torino,Torino,Italy

出　　处：《Neural Regeneration Research》索引2020年第15卷第9期,共8页

基　　金：supported by the research start-up and the MWU’s intramural grant(to MF);the Italian MURST-MIUR foundation(to SG and IP) 摘　　要：Neurotrophins play a major role in the regulation of neuronal growth such as neurite sprouting or regeneration in response to nerve injuries. The role of nerve growth factor, neurotrophin-3, and brain-derived neurotrophic factor in maintaining the survival of peripheral neurons remains poorly understood. In regenerative medicine, different modalities have been investigated for the delivery of growth factors to the injured neurons, in search of a suitable system for clinical applications. This study was to investigate the influence of nerve growth factor, neurotrophin-3 and brain-derived neurotrophic factor on the growth of neurites using two in vitro models of dorsal root ganglia explants and dorsal root ganglia-derived primary cell dissociated cultures. Quantitative data showed that the total neurite length and tortuosity were differently influenced by trophic factors. Nerve growth factor and, indirectly, brain-derived neurotrophic factor stimulate the tortuous growth of sensory fibers and the formation of cell clusters. Neurotrophin-3, however, enhances neurite growth in terms of length and linearity allowing for a more organized and directed axonal elongation towards a peripheral target compared to the other growth factors. These findings could be of considerable importance for any clinical application of neurotrophic factors in peripheral nerve regeneration. Ethical approval was obtained from the Regione Piemonte Animal Ethics Committee ASLTO1(file # 864/2016-PR) on September 14, 2016. 关键词：brain-derived neurotrophic factor DIRECTIONALITY dorsal root ganglia explant nerve growth factor nerve regeneration neurite growth enhancement neurotrophic factors NEUROTROPHIN-3 sensory neurons TORTUOSITY

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Role of neurotrophic factors in enhancing linear axonal growth of ganglionic sensory neurons in vitro

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