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| 1 | 胸腺肽α1对慢性乙型肝炎患者免疫系统的影响显示文摘目的:了解胸腺肽α 1(Tα1)对慢性乙型肝炎患者免疫系统的影响,评价Tα1对慢性乙型病毒性肝炎的疗效。方法:选择60例慢性乙型肝炎患者,其中Tα1组25例,给予Tα1(1.6mg,sc,2次/wk,3-6 mo)治疗,联合组35例,给予Tα1联合贺普丁(0.1g,po,1次/d,3-6 mo)治疗,另选60例作为对照组,仅给予保肝治疗。应用双抗体酶联分析法检测治疗前后血清中IFN-γ和IL-4的浓度,以IFN-γ代表Th1,IL-4代表Th2,从而计算Th1/Th2比值;采用流式细胞仪观察外周血CD_4^+T,CD_s^+T细胞亚群。结果:联合组与对照组比,治疗后肝功能改善显著(P<0.01);联合组乙型肝炎病毒标志物HBsAg或HBeAg阴转(9例)及HBV-DNA定量指标降低(14例),与对照组比,均具有统计学差异(P<0.01;P<0.05).但Tα1组分别与对照组、联合组比,无统计学差异(P>0.05)。Tα1组治疗后CD_4^+T细胞增高(由31.3±2.4增高到36.1±2.5)具有统计学意义(P<0.01),CD_8^+T细胞稍高且CD4^+/CD8^+无明显变化,相差无统计学意义(P>0.05);IFN-γ浓度(由71.3±21.0增高到83.7±21.4)及Th1/Th2比值升高(由0.79±0.2增高到0.98±0.3)均有统计学意义(P均<0.05),但IL-4表达水平变化无统计学意义。结论:Tα1能提高机体的细胞免疫功能,有利于病毒清除和疾病的恢复。 | 段国荣 聂青和 周永兴 王全楚 田长印 刘拉羊 薛红安 | 2003 | 世界华人消化杂志2003，11，6： | 12 |
| 2 | 终末期肝病模型在评估肝硬化患者预后中的价值显示文摘目的:评估终末期肝病模型(model for end stage liver disease, MELD)对肝硬化患者短期和中期生存预测的价值. 方法:对199例肝硬化患者的生存率进行回顾性评估,随访1 a.计算每例患者入院当天的MELD评分与Child-Pugh评分,以受试者特征曲线(ROC)下的面积衡量各评分系统预测患者预后的能力,依据ROC曲线截断值绘制Kaplan-Meier 生存曲线,分析MELD评分与Child-Pugh评分的相关性. 结果:随访3 mo内有37例患者死亡,死亡组MELD与Child-Pugh评分(23.4±9.90,10.8±2.29)与生存组(14.3±4.66,8.68 ±2.21)比较有显著差异(P<0.001);随访第1 a 内有59例患者死亡,死亡组MELD与Child-Pugh评分(20.3 ±9.31,10.3±2.32)与生存组(14.0 ±5.11,8.43±2.23)比较有显著差异(P<0.001);MELD评分与Child-Pugh 评分对3mo预后评估的ROC曲线面积分别为0.826,0.745 (P<0.05),对1 a预后评估的ROC曲线下的面积分别为0.758,0.724(P>0.05);生存分析表明两评分系统在截断值均可有效地分辨3mo或1a内可能生存及死亡的患者(P<0.001); MELD评分与Child-Pugh评分呈显著相关(r=0.69,P<0.001). 结论:MELD评分Child-Pugh评分均可有效地预测肝硬化患者的短期和中期预后,MELD评分短期评估效率优于Child-Pugh分级,能更准确反应病情危急程度,值得临床应用. | 熊伍军 刘菲 赵中辛 邱德凯 | 2004 | 世界华人消化杂志2004，12，5： | 11 |
| 3 | Angiogenesis effect on rat liver after administration of expression vector encoding vascular endothelial growth factor D显示文摘AIM: To verify the expressing efficiency and angiogenesiseffect after administration of expression vector encodingfor vascular endothelial growth factor D in normal andischemic rat liver.METHODS: Ten female S-D rats were administrated withliver tissue dot injection of naked PCHO/hVEGF-D, 50 μg/dot, three dots for each. The same amount of physiologicalsaline was used as control in the neighboring lobe. ForteenS-D rats, using inflow occlusion of left lateral lobe, were dividedinto two groups, seven rats in each group. One was ischemicplasmid group, which received naked plasmid PCHO/hVEGF-D injection of 150 μg. The other received the equal amountof natural saline injection and designed as control. Theexpressions of hVEGF-D in mRNA and protein levels wereidentified by in situ hybridyzation and immunohistochemistry,respectively. Endothelial cells were labeled by the factor VIIIimmunohistochemistrically. The average number of peri-sinusoidal capillaries of each group was calculated andcompared statistically 8 days after injection.RESULTS: A large amount of hVEGF-D in mRNA level wasfound in both normal and ischemic plasmid groups and butnone in their corresponding control groups. The protein ofhVEGF was also highly expressed in both normal and ischemicplasmid groups than in the controls. The mean number ofcapillaries under microscopy (×200) of the plasmid group andcontrol was 10.2±2.78 vs7.1±2.02 (P<0.05), and those ofischemic plasmid group and ischemic control were 7.43+1.72vs 4.71± 1.11 with statistical difference (P<0.05).CONCLUSION; The naked PCHO/hVEGF-D dot injection tonormal, ischemic rat liver can produce comparatively highexpression of hVEGF in both protein and mRNA levels, andprominently increase the number of new capillaries aroundhepatic sinuses. Therefore, it could be another ideal choicefor the treatment of ischemic liver diseases. | Bao-MinShi Xiu-YanWang Qing-LingMu Tai-HuangWu Hong-JunLiu ZhenYang | 2003 | World Journal of Gastroenterology2003，9，2： | 6 |
| 4 | 胰岛素样生长因子测定的临床意义显示文摘胰岛素样生长因子(又称类胰岛素生长因子,简称IGFs)是人体最重要的一类生长因子,参与了一些疾病的病理生理过程,并可作为诊断的参考指标。本文就IGFs的临床应用作一综述。 | 潘爱武 叶剑 于雅杰 杨萍 林星 | 2005 | 现代诊断与治疗2005，16，B12： | 1 |
| 5 | 基因芯片筛选肝硬化相关基因显示文摘目的:应用基因芯片技术研究肝硬化相关基因的基因表达谱. 方法:乙肝肝硬化组织为实验组,以正常肝组织为对照. 按一步法抽提实验组和对照组标本的总RNA并纯化mRNA;将等量的实验组和对照组标本mRNA分别逆转录合成荧光分子掺入的cDNA做探针,混合后杂交1 568点BioDoorChipLiver-16S芯片.经严格洗片后用ScanArray3000 扫描仪扫描芯片获取荧光信号图像,计算机分析后比较两种组织中差异表达的基因. 结果:在1 568种基因中,肝硬化组织与正常肝组织间存在差异表达的基因.所检测的3例临床标本中,2例共有的差异表达基因66-76条(4.2-4.8%),3例共有的差异表达基因28条(1.8%).其中包含有与细胞外基质降解相关的基因,与细胞生长因子有关的基因,与信号传导有关的基因等. 结论:应用基因芯片技术筛选肝硬化相关基因有助于阐明肝硬化发展机制. | 黄海涛 林菊生 余爽 马昕 | 2004 | 世界华人消化杂志2004，12，8： | 0 |
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