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| 1 | One-step generation of complete gene knockout mice and monkeys by CRISPR/Cas9-mediated gene editing with multiple sgRNAs显示文摘CRISPR/Cas9 系统是一个有效编辑基因的方法,但是编辑基因的动物的多数显示出 mosaicism,与编辑仅仅在房间的部分发生。这里,我们证明那单个基因或多重基因能被 Cas9 mRNA 和多重邻近的单个指南的 RNA 的 zygotic 注射完全在老鼠和猴子胚胎击倒(spaced 10-200 bp 分开) 指向仅仅一把单个钥匙每基因的 exon。个别地在 Y 染色体上跟随八基因的指向的删除的 F0 老鼠的 Phenotypic 分析在产生猛烈老鼠表明了这条途径的坚韧性。重要地,这条途径在高效率交付完全的基因大美人(Arntl 上的 100% 并且 91% 在 Prrt2 上) 在猴子胚胎。最后,我们能在一个单个步骤产生一只完全的 Prrt2 猛烈猴子,表明在很快建立编辑基因的猴子模型的这条途径的实用性。 | Erwei Zuo Yi-Jun Cai Kui Li Yu Wei Bang-An Wang Yidi Sun Zhen Liu Jiwei Liu Xinde Hu Wei Wei Xiaona Huo Linyu Shi Cheng Tang Dan Liang Yan Wang Yan-Hong Nie Chen-Chen Zhang Xuan Yao Xing Wang Changyang Zhou Wenqin Ying Qifang Wang Ren-Chao Chen Qi Shen Guo-Liang Xu Jinsong Li Qiang Sun Zhi-Qi Xiong Hui Yang | 2017 | Cell Research2017，27，7： | 21 |
| 2 | Thiolactone copolymer donor gifts organic solar cells a 16.72% efficiency显示文摘Since 1995,bulk-heterojunction organic solar cells consisting of one or two organic donors and one or two organic acceptors have been fighting for high power conversion efficiencies(PCEs)and good stability[1].Until recent years,this next-generation photovoltaic technology starts to offer decent PCEs,shedding the light on commercialization,and attracting great attention again[2-14].Compared w让h the continuously emerging highperformance nonfullerene acceptors,high-performance donors are rare. | Ji Xiong Ke Jin Yufan Jiang Jianqiang Qin Tan Wang Jinfeng Liu Qishi Liu Haili Peng Xiongfeng Li Anxin Sun Xianyi Meng Lixiu Zhang Ling Liu Wenting Li Zhimin Fang Xue Jia Zuo Xiao Yaqing Feng Xiaotao Zhang Kuan Sun Shangfeng Yang Shengwei Shi Liming Ding | 2019 | Science Bulletin2019，64，21： | 17 |
| 3 | Microwave Exposure Impairs Synaptic Plasticity in the Rat Hippocampus and PC12 Cells through Over-activation of the NMDA Receptor Signaling Pathway显示文摘Objective The aim of this study is to investigate whether microwave exposure would affect the N-methyl-D-aspartate receptor(NMDAR) signaling pathway to establish whether this plays a role in synaptic plasticity impairment. Methods 48 male Wistar rats were exposed to 30 m W/cm2 microwave for 10 min every other day for three times. Hippocampal structure was observed through H&E staining and transmission electron microscope. PC12 cells were exposed to 30 m W/cm2 microwave for 5 min and the synapse morphology was visualized with scanning electron microscope and atomic force microscope. The release of amino acid neurotransmitters and calcium influx were detected. The expressions of several key NMDAR signaling molecules were evaluated. Results Microwave exposure caused injury in rat hippocampal structure and PC12 cells, especially the structure and quantity of synapses. The ratio of glutamic acid and gamma-aminobutyric acid neurotransmitters was increased and the intracellular calcium level was elevated in PC12 cells. A significant change in NMDAR subunits(NR1, NR2 A, and NR2B) and related signaling molecules(Ca2+/calmodulin-dependent kinase II gamma and phosphorylated c AMP-response element binding protein) were examined. Conclusion 30 m W/cm2 microwave exposure resulted in alterations of synaptic structure, amino acid neurotransmitter release and calcium influx. NMDAR signaling molecules were closely associated with impaired synaptic plasticity. | XIONG Lu SUN Cheng Feng ZHANG Jing GAO Ya Bing WANG Li Feng ZUO Hong Yan WANG Shui Ming ZHOU Hong Mei XU Xin Ping DONG Ji YAO Bin Wei ZHAO Li PENG Rui Yun | 2015 | Biomedical and Environmental Sciences2015，28，1： | 16 |
| 4 | Cloning and characterization of a potato StAN11 gene involved in anthocyanin biosynthesis regulation显示文摘Anthocyanins are a class of products of plant secondary metabolism and are responsible for tubers color in potato.The biosynthesis of anthocyanins is a complex Researchbiological process,in which multiple genes are involved including structural genes and regulatory genes.In this study,StAN11,a WD40-repeat gene,was cloned from potato cultivar Chieftain(Solanum tuberosum L.).StAN11(HQ599506)contained no intron and its open reading frame(ORF)was 1,029 bp long,encoding a putative protein of 342 amino acids.In order to verify its role in anthocyanin biosynthesis,StAN11 was inserted behind the CaMV-35S promoter of pCMBIA1304 and the recombination vector was introduced into the potato cultivar Désirée plants by Agrobacterium-mediated transformation.The color of transgenic tuber skin was significantly deepened,compared to the wild-type control,which was highly consistent with the accumulation of anthocyanin and expression of StAN11 in transgenic lines tuber skin.Further analysis on the expression of Flavonone-3-hydroxylase(F3H),Dihydroflavonol reductase(DFR),Anthocyanidin synthase(ANS),and Flavonoid 3-O-glucosyl transferase(3GT)in transgenic plants revealed that only DFR was upregulated.This result suggested that StAN11 regulated anthocyanin biosynthesis in potato by controlling DFR expression and accumulation of anthocyanin could be increased through overexpression of StAN11 in the tubers with the genetic background of anthocyanin biosynthesis. | Wang Li Bing Wang Man Wang Min Chen Jing-Ming Yin Ghullam Murtaza Kaleri Rui-Jie Zhang Tie-Niu Zuo Xiong You Qing Yang | 2014 | Journal of Integrative Plant Biology2014，56，4： | 13 |
| 5 | Deficient plastidic fatty acid synthesis triggers cell death by modulating mitochondrial reactive oxygen species显示文摘 | Jian Wu Yuefeng Sun Yannan Zhao Jian Zhang Lilan Luo Meng Li Jinlong Wang Hong Yu Guifu Liu Liusha Yang Guosheng Xiong Jian-Min Zhou Jianru Zuo Yonghong Wang Jiayang Li | 2015 | Cell Research2015，25，5： | 13 |
| 6 | Defined tumor antigen-specific T cells potentiate personalized TCR-T cell therapy and prediction of immunotherapy response显示文摘Personalized immunotherapy targeting tumor-specific antigens(TSAs)could generate efficient and safe antitumor immune response without damaging normal tissues.Although neoantigen vaccines have shown therapeutic effect in clinic trials,precise prediction of neoantigens from tumor mutations is still challenging.The host antitumor immune response selects and activates T cells recognizing tumor antigens.Hence,T cells engineered with T-cell receptors(TCRs)from these naturally occurring tumor antigen-specific T(Tas)cells in a patient will target personal TSAs in his/her tumor.To establish such a personalized TCR-T cell therapy,we comprehensively characterized T cells in tumor and its adjacent tissues by single-cell mRNA sequencing(scRNA-seq),TCR sequencing(TCR-seq)and in vitro neoantigen stimulation.Compared to bystander T cells circulating among tissues,Tas cells were characterized by tumor enrichment,tumor-specific clonal expansion and neoantigen specificity.We found that CXCL13 is a unique marker for both CD4^(+)and CD8^(+)Tas cells.Importantly,TCR-T cells expressing TCRs from Tas cells showed significant therapeutic effects on autologous patient-derived xenograft(PDX)tumors.Intratumoral Tas cell levels measured by CXCL13 expression precisely predicted the response to immune checkpoint blockade,indicating a critical role of Tas cells in the antitumor immunity.We further identified CD200 and ENTPD1 as surface markers for CD4^(^(+))and CD8^(^(+))Tas cells respectively,which enabled the isolation of Tas cells from tumor by Fluorescence Activating Cell Sorter(FACS)sorting.Overall,our results suggest that TCR-T cells engineered with Tas TCRs are a promising agent for personalized immunotherapy,and intratumoral Tas cell levels determine the response to immunotherapy. | Jingjing He Xinxin Xiong Han Yang Dandan Li Xuefei Liu Shuo Li Shuangye Liao Siyu Chen Xizhi Wen Kuai Yu Lingyi Fu Xingjun Dong Kaiyu Zhu Xiaojun Xia Tiebang Kang Chaochao Bian Xiang Li Haiping Liu Peirong Ding Xiaoshi Zhang Zhenjiang Liu Wende Li Zhixiang Zuo Penghui Zhou | 2022 | Cell Research2022，32，6： | 10 |
| 7 | Changes of microstructure of different quench sensitivity 7,000 aluminum alloy after end quenching显示文摘The quench sensitivity and their influential factors of 7,021, 7,085, and 7,050 alloys were investigated by the end quenching test method and the measurement of electrical conductivity, hardness, and microstructure after aging. The results indicate that 7,050 alloy has the largest changes with hardness decreasing from HV 199 to HV 167,and electrical conductivity increases from 16.6 to18.2 MS m-1when the distance from quenched end increases from 2 to 100 mm. Alloys 7,085 and 7,021 have relatively smaller changes. According to the relationship between the hardness and electrical conductivity of a supersaturated solid solution, 7,050 alloy has higher quench sensitivity than 7,085 and 7,021 alloys. The microstructure of 7,050 alloy with higher major alloy element(Zn + Mg + Cu) addition and Cu element addition is mostly affected by the changes of distance from quenched end. In 7,050 alloy, the size of intragranular precipitates is from about 10–200 nm, and the(sub) grain boundary precipitates are about 20–300 nm. Alloy 7,085 with lower Cu content is moderately affected, while 7,021 is least affected. It is found that with the increase of distance from quenched end, quenched-induced precipitate preferentially nucleates and grows in the(sub) grain boundary and then on the pre-existing Al3 Zr particles. | Zhi-Hui Zhang Bai-Qing Xiong Shu-Feng Liu Bao-Hong Zhu Yu-Ting Zuo | 2014 | Rare Metals2014，33，3： | 10 |
| 8 | The localization of type 2 diabetes susceptibility gene loci in northern Chinese Han families显示文摘We conducted a genome-wide scan, in which 358 well distributed fluorescent dye-labeled microsatellite marker sets were applied in 32 Chinese Han type 2 diabetes families from Northern China to search for the susceptibility gene loci. The data collected from screening all the chromosomes of genome were genotyped by using genescan and genotyping software, then, parametric and non-parametric multipoint test, and affected sib-pair analysis as well, were used to analyze the data. We identified some susceptibility gene loci residing in chromosomes 1,12,18,20, respectively, or precisely, located around D1S214, D1S207, D1S218, D1S235, D12S336, D18S61 and D20S118. The comparison of this result with those from other regions and races reflected the complexity and heterogeneity of type 2 diabetes. | ZHAO Jinying WANG Heng XIONG Momiao HUANG Wei ZUO Jin CHEN Zhu QIANG Boqin SUN Qi LI Yuxiu LIU Qiuying DU Weinan CHEN Jialun DING Wei YUAN Wentao ZHAO Yang XU Hongyan JIN Li FANG Fude | 2000 | Chinese Science Bulletin2000，45，19： | 8 |
| 9 | A comparative study of fuzzy weights of evidence and random forests for mapping mineral prospectivity for skarn-type Fe deposits in the southwestern Fujian metallogenic belt, China显示文摘Recent studies have pointed out that the widespread iron deposits in southwestern Fujian metallogenic belt(SFMB)(China) are skarn-type deposits associated with the Yanshanian granites. There is still excellent potential for mineral exploration because large areas in this belt are covered by forest. A new predictive model for mapping skarn-type Fe deposit prospectivity in this belt was developed and focused on in this study, using five criteria as evidence:(1) the contact zones of Yanshanian granites(GRANITE);(2) the contact zones within the late Paleozoic marine sedimentary rocks and the carbonate formations(FORMATION);(3) the NE-NNE-trending faults(FAULT);(4) the zones of skarn alterations(SKARN); and(5) the aeromagnetic anomaly(AEROMAGNETIC). The fuzzy weights of evidence(FWof E) method, developed from the classical weights of evidence(Wof E) and based on fuzzy sets and fuzzy probabilities, could provide smaller variances and more accurate posterior probabilities and could effectively minimize the uncertainty caused by omitted or wrongly assigned data and be more flexible than the Wof E. It is an efficient and widely used method for mineral potential mapping. Random forests(RF) is a new and useful method for data-driven predictive mapping of mineral prospectivity method, and needs further scrutiny. Both prospectivity results respectively using the FWof E and RF methods reveal that the prediction model for the skarn-type Fe deposits in the SFMB is successful and efficient. Both methods suggested that the GRANITE and FORMATION are the most valuable evidence maps, followed by SKARN, AEROMAGNETIC, and FAULT. This is coincident with the skarn-type Fe deposit mineral model in the SFMB. The unstable performance experienced when FORMATION was omitted might indicate that the highest uncertainty and risk in follow-up exploration is related to the sequences. In addition, the performance of the RF method for the skarn-type Fe deposits prospectivity in the SFMB is better than the FWof E; therefore, it could be used to guide further exploration of skarn-type Fe prospects in the SFMB. | ZHANG Zhen Jie ZUO Ren Guang XIONG Yi Hui | 2016 | Science China Earth Sciences2016，59，3： | 7 |
| 10 | Prevalence of late-onset hypogonadism among middle-aged and elderly males in China:results from a national survey显示文摘This study aimed to propose an operational definition of late-onset hypogonadism(LOH)that incorporates both clinical symptoms and serum testosterone measurements to evaluate the prevalence of LOH in aging males in China.A population-based sample of 6296 men aged 40 years-79 years old was enrolled from six representative provinces in China.Serum total testosterone(TT),sex hormone-binding globulin(SHBG),and luteinizing hormone(LH)were measured and free testosterone(cFT)was calculated.The Aging Males’Symptoms(AMS)scale was used to evaluate the LOH symptoms.Finally,5078 men were included in this analysis.The TT levels did not decrease with age(P=0.59),and had no relationship with AMS symptoms(P=0.87 for AMS total score,P=0.74 for≥3 sexual symptoms).The cFT levels decreased significantly with age(P<0.01)and showed a negative association with the presence of≥3 sexual symptoms(P=0.03).The overall estimated prevalence of LOH was 7.8%(395/5078)if a cFT level<210 pmol l−1 combined with the presence of≥3 sexual symptoms was used as the criterion of LOH.Among them,26.1%(103/395)and 73.9%(292/395)had primary and secondary hypogonadism,respectively.After adjustment for confounding factors,primary and secondary hypogonadism was positively related to age and comorbidities.Body mass index was an independent risk factor for secondary hypogonadism.The results suggest that the AMS total score is not an appropriate indicator for decreased testosterone,and that the cFT level is more reliable than TT for LOH diagnosis.Secondary hypogonadism is the most common form of LOH. | Yi-Jun Liu Xu-Bo Shen Na Yu Xue-Jun Shang Yi-Qun Gu Lian-Dong Zuo Cheng-Liang Xiong Zhen Ye Yuan-Zhong Zhou | 2021 | Asian Journal of Andrology2021，23，2： | 6 |
| 11 | A transient expression system in soybean mesophyll protoplasts reveals the formation of cytoplasmic GmCRY1 photobody-like structures显示文摘Soybean(Glycine max(L.) Merr.), grown for its plant oils and proteins, is one of the most important crops throughout the world.Generating stable and heritable transgenic soybeans is relatively inefficient;therefore, there is an urgent need for a simple and high-efficient transient transformation method by which to enable the investigation of gene functions in soybeans, which will facilitate the elucidation and improvement of the molecular mechanisms regulating the associated agronomic traits. We established a system of transient expression in soybean mesophyll protoplasts and obtained a high level of protoplast transfection efficiency(up to 83.5%). The subcellular activity of the protoplasts was well preserved, as demonstrated by the dynamic formation of GmCRY nucleus photobodies(NPs) and/or cytoplasmic photobody-like structures(CPs) in response to blue light.In addition, we showed that GmCRY1b CPs colocalized with GmCOP1b, a co-ortholog of Arabidopsis thaliana CONSTITUTIVE PHOTOMORPHOGENIC 1(COP1), which provided new insight into the potential roles of GmCRY1s in the cytoplasm. | Lu Xiong Cong Li Hongyu Li Xiangguang Lyu Tao Zhao Jun Liu Zecheng Zuo Bin Liu | 2019 | Science China(Life Sciences)2019，62，8： | 6 |
| 12 | Low-strain TiP_(2)O_(7) withthree-dimensionalionchannelsas long-life and high-rate anode material for Mg-ion batteries显示文摘Rechargeable magnesium batteries are identified as a promising next-generation energy storage system,but their development is hindered by the anode−electrolyte−cathode incompatibilities and passivation of magnesium metal anode.To avoid or alleviate these problems,the exploitation of alternative anode materials is a promising choice.Herein,we present titanium pyrophosphate(TiP_(2)O_(7))as anode materials for magnesium-ion batteries(MIBs)and investigate the effect of the crystal phase on its magnesium storage performance.Compared with the me-tastable layered TiP_(2)O_(7),the thermodynamically stable cubic TiP_(2)O_(7) displays a better rate capability of 72 mAh g^(−1) at 5000 mA g^(−1).Moreover,cubic TiP_(2)O_(7) exhibits excellent cycling stability with the capacity of 60 mAh g^(−1) after 5000 cycles at 1000 mA g^(−1),which are better than pre-viously reported Ti-based anode materials for MIBs.In situ X-ray diffraction technology confirms the single-phase magnesiumion inter-calation/deintercalation reaction mechanism of cubic TiP_(2)O_(7) with a low volume change of 3.2%.In addition,the density functional theory calcu-lation results demonstrate that three-dimensional magnesiumion diffu-sion can be allowed in cubic TiP_(2)O_(7) with a low migration energy barrier of 0.62 eV.Our work demonstrates the promise of TiP_(2)O_(7) as high-rate and long-life anode materials for MIBs and may pave the way for further development of MIBs. | Fangyu Xiong Yalong Jiang Li Cheng Ruohan Yu Shuangshuang Tan Chen Tang Chunli Zuo Qinyou An Yunlong Zhao Jean-Jacques Gaumet Liqiang Mai | 2022 | Interdisciplinary Materials2022，1，1： | 3 |
| 13 | Maternal sleep deprivation induces gut microbial dysbiosis and neuroinflammation in offspring rats显示文摘Maternal sleep deprivation(MSD)is a global public health problem that affects the physical and mental development of pregnant women and their newborns.The latest research suggests that sleep deprivation(SD)disrupts the gut microbiota,leading to neuroinflammation and psychological disturbances.However,it is unclear whether MSD affects the establishment of gut microbiota and neuroinflammation in the newborns.In the present study,MSD was performed on pregnant SpragueDawley rats in the third trimester of pregnancy(gestational days 15-21),after which intestinal contents and brain tissues were collected from offspring at different postnatal days(P1,P7,P14,and P56).Based on microbial profiling,microbial diversity and richness increased in pregnant rats subjected to MSD,as reflected by the significant increase in the phylum Firmicutes.In addition,microbial dysbiosis marked by abundant Firmicutes bacteria was observed in the MSD offspring.Furthermore,quantitative real-time polymerase chain reaction(q RT-PCR)and enzyme-linked immunosorbent assay(ELISA)showed that the expression levels of proinflammatory cytokines interleukin 1β(IL-1β)and tumor necrosis factorα(TNF-α)were significantly higher in the MSD offspring at adulthood(P56)than in the control group.Through Spearman correlation analysis,IL-1βand TNF-αwere also shown to be positively correlated with Ruminococcus_1 and Ruminococcaceae_UCG-005 at P56,which may determine the microbiota-host interactions in MSDrelated neuroinflammation.Collectively,these results indicate that MSD changes maternal gut microbiota and affects the establishment of neonatal gut microbiota,leading to neuroinflammation in MSD offspring.Therefore,understanding the role of gut microbiota during physiological development may provide potential interventions for cognitive dysfunction in MSD-impacted offspring. | Zheng-Yu Yao Xiao-Huan Li Li Zuo Qian Xiong Wen-Ting He Dong-Xu Li Zhi-Fang Dong | 2022 | Zoological Research2022，43，3： | 3 |
| 14 | Chelerythrine chloride binding of guanosine in aqueous solution显示文摘在这个工作,在 chelerythrine (CHE ) 和海鸟粪正弦之间的相互作用用 UV– 被学习;在各种各样的温度的力和荧光大小。UV–力系列证明增加的海鸟粪正弦集中导致减少的吸收紧张和 CHE E 吸收乐队(267 nm ) 的红移动。荧光系列被适合到线性分析,产出 1.04 × 的一个有约束力的常数;在有海鸟粪的 CHE 的 298.15 K 的 10 4 L/mol 正弦。而且,与,,并且相互作用应该是熵驱动的并且 exterothermic。 | Fan Liu Jian Bin Zhang Zheng Fu Zhang Xiao Yan Zhang Zuo Peng Du Xiong Hui Wei | 2009 | Chinese Chemical Letters2009，20，1： | 3 |
| 15 | Statistical Characteristics of Metastable Pitting of 316 Stainless Steel显示文摘The statistical characteristics of the Current fluctuations during metastable pitting of 316L stain- less steel in NaCI solution were studied using potentiostatic method. The growth rates and peak currents of metastable pits followed log-normal distributions. As potential and chloride concentration increased, both growth rate and peak current of metastable pits increased. The lifetime of metastable pits also followed log-normal distribution. and was almost not affected by potential. Higher growth rates did not definitely result in larger metastable pits. A certain propagation rate range was found beneficial for the micropit to remain growth. At constant potential, the maximum peak currents in different time intervals during a potentiostatic test followed extreme distribution. The statistical characteristics of metastable pitting may be used to predict the tendency of pitting corrosion. | Yu ZUO, Haiou DU and Jinping XIONG (Dept. of Applied Chemistry, Beijing University of Chemical Technology, Beijing 100029, China) | 2000 | Journal of Materials Science & Technology2000，16，3： | 3 |
| 16 | Sequencing, Polymorphism and Expression Profile Analysis of Porcine HexokinaseⅡ(HK2) Gene显示文摘Hexokinase Ⅱ has been demonstrated to play the role of a key enzyme member in the glycolysis reaction. It catalyzes theconversion of glucose to glucose-6-phosphate, thus committing glucose to the glycolytic pathway. In this paper, thepartial exons and introns 10, 11, 13 and 14 of the porcine HK2 gene were cloned and sequenced by comparative genomics.Comparative sequencing of three pig breeds revealed ten putative single-nucleotide polymorphisms (SNPs), one of whichin intron 10 with differing bases (G981A ) is within the restriction site for enzyme Msp Ⅰ. Distribution of Msp Ⅰ -RFLPgenotype and allele frequencies among different pig breeds were studied. By association analysis between Msp Ⅰ PCR-RFLP polymorphism (AA, AB, BB genotypes of HK2 gene intron 10) and some meat quality and carcass traits in F2 group,which was constructed by our laboratory, a significant difference of pig average backfat at rump was found between ABand BB genotypes (P < 0 05) in F2 group. In addition, the pattern of expression of HK2 in a variety of tissues in pig was alsodetermined using semi-quantitative RT-PCR. The expression of HK2 mRNA was detected only in pig skeletal muscle. | WANG Jun DENG Chang-yan XIONG Yuan-zhu ZUO Bo CHENG Huan-chen LI Feng-e LEI Ming-gang ZHENG Rong JIANG Si-wen | 2006 | Agricultural Sciences in China2006，5，5： | 3 |
| 17 | Numerical flow simulation in the post-endoscopic sinus surgery nasal cavity显示文摘 | Guanxia Xiong Jiemin Zhan Kejun Zuo Jianfeng Li Liangwan Rong Geng Xu | 2008 | Medical & Biological Engineering & Computing2008，，11： | 2 |
| 18 | Intrauterine growth restriction alters nutrient metabolism in the intestine of porcine offspring显示文摘Background:Intrauterine growth restriction(IUGR)has negative impacts on the postnatal survival,growth and development of humans and animals,with not only on newborns but also adulthood.However,the characteristics for nutrient digestion and absorption in IUGR offspring are still largely unknown.Therefore,the normal birth weight(NBW)and IUGR growing pigs were used in this study to investigate their differences in nutrient utilization,with an expectition for further nutritional optimization of the IUGR offspring during their later life.Methods:Twelve IUGR and 12 NBW growing pigs were fitted with catheters in their portal vein to measure blood flow rate as well as nutrients and metabolites in plasma.The digestibilities of nutrients in different intestinal segments,and bacterial fermentation in the large intestine were examined to reveal the characteristics of nutrients utilization in IUGR versus NBW pigs.Results:The rate of portal venous blood flow did not differ beween IUGR and NBW pigs.Plasma concentrations of total cholesterol,triglycerides and glucose were much lower but those of urea were higher in the portal vein of IUGR pigs,compared with the NBW pigs.The ileal digestibility of dry matter,gross energy and starch were lower in IUGR pigs than in NBW pigs.IUGR increased hindgut microbial diversity and bacterial fermentation activity in the caecum.In vitro cross-fermentation of ileal digesta by caecal microbes of NBW and IUGR pigs showed that gas production was much higher for IUGR ileal digesta regardless of the source of caecal inocula.Conclusion:IUGR impairs the nutrient digestion and absorption in small intestine,reduces caecal microbial diversity and promotes bacterial fermentation in the large intestine during the growing phase.These findings aid in our understanding of nutrient metabolism in IUGR pigs and provide the basis for future nutritional interventions. | Tiantian Li Shimeng Huang Long Lei Shiyu Tao Yi Xiong Guoyao Wu Jie Hu Xiongkun Yuan Shengjun Zhao Bin Zuo Hongjian Yang Yingping Xiao Gang Lin Junjun Wang | 2021 | Journal of Animal Science and Biotechnology2021，12，2： | 2 |
| 19 | cDNA Cloning,Sequence Analysis of the Porcine LIM and Cysteine-rich Domain 1 Gene显示文摘秘鲁利玛机场之代号领域蛋白质是在房间生长的重要管理者,房间命运决心,细胞分化并且由他们和各种各样的结构的蛋白质,家族 ases 和 transcriptional 的相互作用房间细胞骨架改变管理者。使用分子生物学与结合了在克隆的 silico,我们克隆猪秘鲁利玛机场之代号和充满半胱氨酸的领域的完全的编码顺序编码 363 氨基酸蛋白质的 1 基因(LMCD1 ) 。LMCD1 蛋白质的估计的分子量是有 8.39 的 pI 的 40,788 Da。高度在骨胳的肌肉和心肌被表示被发现。排列分析表明推出的蛋白质顺序分别地与它的人,老鼠和老鼠对应物的分享 86% , 91% 和 93% 相同。 LMCD1 蛋白质被生物信息学软件预言在N终端区域包含新奇的充满半胱氨酸的域,在C终端区域的二秘鲁利玛机场之代号域,九个潜在的蛋白激酶 C 磷酸化地点,七个酷蛋白 kinase II 磷酸化地点,一个酷氨酸 kinase 磷酸化地点,七个 N-glycosylation 和 N-myristoylation 地点和一个单个潜在的 N-glycosylation 地点,它类似于蛋白质的人的对应物。系统树被从不同种类排列 LIM 领域的氨基酸顺序构造。另外,四个基础变化被中国 Meishan 猪把大白猪的序列与那些作比较检测。G294A 变化地点被聚合酶链 reaction-single-strand 符合构造多型性分析证实。它的等位基因频率在五个猪品种被学习。 | Jun WANG Chang-Yan DENG Yuan-Zhu XIONG Bo ZUO Lei XING Feng-E LI Ming-Gang LEI Rong ZHENG Si-Wen JIANG | 2005 | Acta Biochimica et Biophysica Sinica2005，37，12： | 2 |
| 20 | Neuroprotective effects of olanzapine against rotenone-induced toxicity in PC12 cells显示文摘Olanzapine is an antipsychotic drug used to treat patients with schizophrenia due to its lower incidence of extrapyramidal symptoms.Previous studies have shown that olanzapine activates AMP-activated protein kinase(AMPK),and induce autophagy in SH-SY5Y cell line.In this study,we investigated whether olanzapine protected against rotenone-induced neurotoxicity in PC12 cells.We showed that treatment with olanzapine increased the phosphorylation of AMPK in both dose-and time-dependent manners in PC12 cells.In addition,olanzapine activated autophagy and increased autophagic vacuoles.Furthermore,olanzapine pretreatment could protect PC12 cells from rotenone-induced apoptosis.Besides,olanzapine pretreatment could suppress the rotenone-induced depolarization of mitochondrial potential and thus protect the cells.Moreover,pretreatment with speci?c AMPK inhibitor compound C or with autophagy inhibitor 3-methyladenine impaired the protective effect of olanzapine on rotenone-treated PC12 cells.In summary,our results show for the?rst time that olanzapine ameliorates rotenone-induced injury by activating autophagy through AMPK pathway. | Ya-jie Xiong Yun-zhen Song Ying Zhu Wen-qing Zuo Yi-fan Zhao Xiao Shen Wen-juan Wang Ya-li Liu Jun-chao Wu Zhong-qin Liang | 2020 | Acta Pharmacologica Sinica2020，41，4： | 2 |
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