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| 1 | Growth performance and physiological parameters of the second generation selected and control groups of Pinctada martensii显示文摘In May of 2007,the second generation selected (SS) and control (SC) groups of pearl oyster Pinctada martensii were established by selecting 10% breeders with the largest and mean shell length,respectively,from the first generation selected group.Growth performance of the SS and SC groups were compared on the basis of measurement data at Days 8,18,60,95,195 and 365.On Day 365,100 individuals (60.0–75.0 mm at shell length) were sampled from each group and then subjected to the experiment where physiological parameters such as filtrate rate,oxygen consumption and ammonia excretion were measured at 15,20,25 and 30°C.The results show that the SS group had significantly larger mean shell length and shell height than the SC group at Days 8,18,60,95,195 and 365 (P <0.05).The genetic gains at different ages varied from 6.0% to 17.0% for shell length and 5.7% to 14.6% for shell height,respectively.At 15,20,25 and 30 ° C,the SS groups had significantly larger filtrate rate than the SC group (P <0.05).At 15 and 25 °C,the differences in oxygen consumption rate between the SS and SC groups were not significant (P >0.05).At 20 and 30 °C,however,the oxygen consumption rate of the SS group was significantly larger than the SC group (P <0.05).At 15,20,25 and 30 °C,there were no significant differences in ammonia excretion rate between the SS and SC groups (P >0.05).The present results indicate that there existed considerable genetic variability in the base population and a further selection could be likely fruitful.Mass selection for faster growth might produce animals that had higher intake of metabolizable energy by virtue of faster filtrating behavior. | DENG Yuewen YU Ziniu DU Xiaodong WANG Qingheng FU Shao | 2011 | Acta Oceanologica Sinica2011，30，2： | 10 |
| 2 | Genomic landscape and genetic manipulation of the black soldier fly Hermetia illucens,a natural waste recycler显示文摘The black soldier fly(BSF),Hermetia illucens(Diptera:Stratiomyidae),is renowned for its bioconversion of organic waste into a sustainable source of animal feed.We report a high-quality genome of 1.1 Gb and a consensus set of 16,770 gene models for this beneficial species.Compared to those of other dipteran species,the BSF genome has undergone a substantial expansion in functional modules related to septic adaptation,including immune system factors,olfactory receptors,and cytochrome P450s.We further profiled midgut transcriptomes and associated microbiomes of BSF larvae fed with representative types of organic waste.We find that the pathways related to digestive system and fighting infection are commonly enriched and that Firmicutes bacteria dominate the microbial community in BSF across all diets.To extend its potential practical applications,we further developed an efficient CRISPR/Cas9-based gene editing approach and implemented this to yield flightless and enhanced feeding capacity phenotypes,both of which could expand BSF production capabilities.Our study provides valuable genomic and technical resources for optimizing BSF lines for industrialization. | Shuai Zhan Gangqi Fang Minmin Cai Zongqing Kou Jun Xu Yanghui Cao Liang Bai Yixiang Zhang Yongmao Jiang Xingyu Luo Jian Xu Xia Xu Longyu Zheng Ziniu Yu Hong Yang Zhijian Zhang Sibao Wang Jeffery K.Tomberlin Jibin Zhang Yongping Huang | 2020 | Cell Research2020，30，1： | 9 |
| 3 | The role of β18-β19 loop structure in insecticidal activity of Cry1Ac toxin from Bacillus thuringiensis显示文摘The β18-β19 loop in domain III of Cry1Ac toxin is unique among Bacillus thuringiensis Cry proteins. In this study, the role of the loop structure in insecticidal activity of Cry1Ac toxin was investigated. Alanine scanning mutations within the loop were initially generated and most mutants were over-expressed and reduced toxicity at different degrees, except mutant N546A that showed almost 2 times enhanced toxicity against Helicoverpa armigera larvae. Further mutagenic analysis of N546 revealed that a charged amino acid in this position would cause very unfavorable influence on insecticidal activity. In addition, the deletion of N546 led to protein instability because of destruction of the loop integrity. Besides, mutant W544F was much more toxic than W544Y, indicating that hydrophobic nature of the position was important for maintaining the stability and activity of Cry1Ac protein. These findings are the first biological evidence for a structural function of β18-β19 loop in insecticidal activity of the Cry1Ac toxin. | XIA LiQiu WANG FaXiang DING XueZhi ZHAO XinMin FU ZuJiao QUAN MeiFang YU ZiNiu | 2008 | Chinese Science Bulletin2008，53，20： | 4 |
| 4 | Analysis of the efficacy of transcatheter arterial infusion chemotherapy in the treatment of pancreatic carcinoma显示文摘Objective:To evaluate the clinical efficacy of infusion of gemcitabine(GEM) and fluorouracil(5-FU)through the celiac artery and superior mesenteric artery in the treatment of pancreatic carcinoma(PC).Methods:We analyzed 20 patients diagnosed clinically or pathologically with PC,without metastases,who had an estimated survival duration of>3 months in our department from May 2009 to December 2014.Nine patients were treated directly without surgical resection of the tumor,while the other 11 patients were treated after surgery.In all patients,the femoral artery was punctured using the Seldinger technique,and a catheter was placed in the opening of the celiac artery or the superior mesenteric artery.We administered 500 mg/m2 GEM and 500 mg/m2 5-FU.Observational data included data on clinical efficacy and survival rates during the follow-up period of 3-72 months.Results:Twenty patients were treated 85 times with transcatheter arterial infusion chemotherapy(TAI).The survival rates were 80%,40%,35%,20%,10%,and 5% at 3,6,12,24,and 72 months,respectively.Conclusion: TAI chemotherapy with GEM and 5-FU may be a therapeutic option for the treatment of PC. | Chunhui Nie Yuelin Zhang Guanhui Zhou Tanyang Zhou Tongyin Zhu Baoquan Wang Shengqun Chen Hongliang Wang Ziniu Yu Li Jing Junhui Sun | 2021 | Journal of Interventional Medicine2021，4，1： | 4 |
| 5 | Heterotrophic nitrification and aerobic denitrification by the bacterium Pseudomonas stutzeri YZN-001显示文摘 | Jibin Zhang Pengxia Wu Bo Hao Ziniu Yu | 2011 | Bioresource Technology2011，，21： | 2 |
| 6 | 澜沧江-湄公河流域的温度变化及极端温度事件的未来预估显示文摘研究目的:澜沧江-湄公河流域以农业生产为主要经济来源,气候变化会对该地区人民的生产生活及生命财产安全造成严重的影响,对此流域21世纪的温度和极端温度事件进行预估,可以为灾害防控和政策制定提供科学性参考。创新要点:利用分位数订正法对WRF降尺度数据进行订正,对澜沧江-湄公河流域21世纪的温度变化进行预估。研究方法:运用分位数订正法对模拟和预估数据进行订正,与观测数据进行对比评估,在此基础上对2016–35, 2046–65和2080–99的温度进行预估。重要结论:21世纪澜沧江-湄公河流域温度呈现持续升高的趋势。干季时,北部高海拔地区升温速度较快;湿季时,下游地区升温明显。干湿季最主要的空间模态为全区一致型;干季的第二空间模态为东西反向变化,湿季为南北反向变化。极端高温日数增加,流域下游增加较快;极端低温日数减少,2016–35年的减少最为明显。 | SUN Chang XIAO Ziniu SUN Jianqi YU Entao | 2020 | Atmospheric and Oceanic Science Letters2020，13，1： | 2 |
| 7 | Comparison of Three Microwave/Chemical Pretreatment Processes for Enzymatic Hydrolysis of Rice Straw显示文摘 | Shengdong Zhu Yuanxin Wu Ziniu Yu Cunwen Wang Faquan Yu Siwei Jin Yigang Ding Ru’an Chi Jintao Liao Yan Zhang | 2005 | Biosystems Engineering2005，，3： | 1 |
| 8 | Comparison of three microwave/chemical pretreatment processes for enzymatic hydrolysis of rice straw显示文摘 | Zhu Shengdong Wu Yuanxin Yu Ziniu | 2006 | Biosystems Engineering2006，93，30： | 1 |
| 9 | Pretreatment by microwave/alkali of rice straw and its enzymic hydrolysis显示文摘 | Shengdong Zhu Yuanxin Wu Ziniu Yu Jintao Liao Yan Zhang | 2005 | Process Biochemistry2005，，9： | 1 |
| 10 | Pretreatment by microwave/alkali of rice straw and its enzymic hydrolysis显示文摘 | Shengdong Zhu Yuanxin Wu Ziniu Yu | 2005 | Process Biochemistry2005，40，： | 1 |
| 11 | Pretreatment by microwave/alkali of rice straw and its enzymic hydrolysis显示文摘 | Shengdong Zhu Yuanxin Wu Ziniu Yu | 2005 | Process Biochemistry2005，40，： | 1 |
| 12 | Soil microbial activity measured by microcalorimetry in response to long-term fertilization regimes and available phosphorous on heat evolution显示文摘 | Shixue Zheng Junli Hu Ke Chen Jun Yao Ziniu Yu Xiangui Lin | 2009 | Soil Biology and Biochemistry2009，，： | 1 |
| 13 | Optimization of process parameters for poly-γ-glutamate production under solid state fermentation from Bacillus subtilis CCTCC202048 显示文摘 | Xu Jian Chen Shouwen Yu Ziniu | 2005 | Process Biochemistry2005，40，： | 1 |
| 14 | Elevated CO 2 alters larval proteome and its phosphorylation status in the commercial oyster, Crassostrea hongkongensis显示文摘 | R. Dineshram V. Thiyagarajan Ackley Lane Yu Ziniu Shu Xiao Priscilla T. Y. Leung | 2013 | Marine Biology2013，，8： | 1 |
| 15 | Genetic analysis of selected strains of eastern oyster ( Crassostrea virginica Gmelin) using AFLP and microsatellite markers 显示文摘 | Yu Ziniu Guo Ximing | 2005 | Marine Biotechnology2005，6，6： | 1 |
| 16 | Comparison of three microwave/chemical pretreatment processes for enzymatic hydrolysis of rice straw 显示文摘 | Zhu Shengdong Wu Yuanxin Yu Ziniu | 2006 | Biosystems Engineering2006，93，3： | 1 |
| 17 | Tetrapioid induction of Crassostrea hongkongensis and C.sikamea by inhibiting the polar body 1 release in diploid fertilized eggs显示文摘The production of an all-triploid population by mating tetrapioid males with diploid females is the best and most fundamental method for the large-scale production of triploid oysters.Obtaining a stable tetrapioid population is essential for guaranteed production in industrialized triploid cultivation.C.hongkongensis and C.sikamea are important oyster breeding species in southern China,and have great economic value.However,there are not any published data on inducing tetrapioid C.hongkongensis or C.sikamea.Therefore,we investigated tetrapioid induction in these two oyster species by inhibiting the PB1 release in diploid fertilized eggs using Cytochalasin B(CB)under 31℃,15%o salinity.The results confirmed that the optimal tetrapioid induction conditions for C.hongkongensis were a CB concentration of 0.50 mg/L with induction starting at 9.0 min after fertilization,and stopping at 21.0 min after fertilization;the induction efficiency index reached 0.123 under these conditions.The optimal tetrapioid induction conditions for C.sikamea were a CB concentration of 0.50 mg/L,with induction starting at 7.5 min after fertilization and stopping at 18 min after fertilization;the induction efficiency index could be as high as 0.281 under these conditions.However,we confirmed that the tetrapioid rate decreased with larval growth,and no tetrapioids were detected in the juvenile period of either C.hongkongensis or C.sikamea.This may be attributed to the very low survival of the tetrapioid larvae induced by this method,especially as most tetrapioid larvae died during the first three days.In summary,it is simple to directly induce tetrapioid C.hongkongensis and C.sikamea larvae by inhibiting the PB1 release of diploid zygotes,but the low survival rate makes it challenging to obtain viable juvenile tetrapioids. | Yanping Qin Zohaib Noor Xingyou Li Yuehuan Zhang Ziniu Yu Haitao Ma Jun Li Yinyin Zhou Riguan Mo | 2021 | Marine Life Science & Technology2021，3，4： | 1 |
| 18 | Heterotrophic nitrification and aerobic denitrification by the bacterium Pseudomonas stutzeri YZN-001显示文摘 | Jibin Zhang Pengxia Wu Bo Hao Ziniu Yu | 2011 | Bioresource Technology2011，，21： | 1 |
| 19 | Syn- thesis and solution properties of novel comb-shaped acrylamide copolymers 显示文摘 | Zhou ChenNun Yang Weimin Yu Ziniu | 2011 | Polymer Bulletin2011，66，3： | 1 |
| 20 | Pretreatment by microwave/alkali office straw and its enzymic hydrolysis显示文摘 | Zhu Shengdong Wu Yuanxin Yu Ziniu | 2005 | Process Biochemistry2005，40，9： | 1 |
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