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| 1 | Evaluation of three-dimensional biofilms on antibacterial bonding agents containing novel quaternary ammonium methacrylates显示文摘Antibacterial adhesives are promising to inhibit biofilms and secondary caries.The objectives of this study were to synthesize and incorporate quaternary ammonium methacrylates into adhesives,and investigate the alkyl chain length effects on three-dimensional biofilms adherent on adhesives for the first time.Six quaternary ammonium methacrylates with chain lengths of 3,6,9,12,16 and 18were synthesized and incorporated into Scotchbond Multi-Purpose.Streptococcus mutans bacteria were cultured on resin to form biofilms.Confocal laser scanning microscopy was used to measure biofilm thickness,live/dead volumes and live-bacteria percentage vs.distance from resin surface.Biofilm thickness was the greatest for Scotchbond control;it decreased with increasing chain length,reaching a minimum at chain length 16.Live-biofilm volume had a similar trend.Dead-biofilm volume increased with increasing chain length.The adhesive with chain length 9 had 37%live bacteria near resin surface,but close to 100%live bacteria in the biofilm top section.For chain length 16,there were nearly 0%live bacteria throughout the three-dimensional biofilm.In conclusion,strong antibacterial activity was achieved by adding quaternary ammonium into adhesive,with biofilm thickness and live-biofilm volume decreasing as chain length was increased from 3 to 16.Antibacterial adhesives typically only inhibited bacteria close to its surface;however,adhesive with chain length 16 had mostly dead bacteria in the entire three-dimensional biofilm.Antibacterial adhesive with chain length 16 is promising to inhibit biofilms at the margins and combat secondary caries. | Han Zhou Michael D Weir Joseph M Antonucci Gary E Schumacher Xue-Dong Zhou Hockin H K Xu | 2014 | International Journal of Oral Science2014，6，2： | 5 |
| 2 | In vitro Ruminal Gas Production Kinetics of Four Fodder Trees Ensiled With or Without Molasses and Urea显示文摘This study investigated if the addition of urea (U), molasses (M) or their 1:1 (v/v) mixture during ensiling increases the nutritional value of forage from four fodder trees (Prunus persica, Leucaena esculenta, Acacia farnesiana, and Prunus domestica). Forage samples of fodder trees were collected in triplicate (three individual samples of each species) and subjected to an in vitro gas production (GP) procedure. Fermentation at 24 h (GP 24), short-chain volatile fatty acids (SCFA), and microbial crude protein production (MCP), in vitro organic matter digestibility (OMD), metabolizable energy (ME) and dry matter degradability (DMD) were estimated. Forage samples were incubated for 72 h in an incubator at 39oC and the volume of GP was recorded at 2, 4, 6, 8, 10, 12, 24, 48, and 72 h of incubation using the reading pressure technique. The rumen fermentation profiles were highest for P. persica, which showed the highest (P<0.0001) DMD, ME, OMD, SCFA, GP 24 and MCP. On the other hand L. esculenta had the lowest (P<0.0001) DMD, SCFA, MCP; P. domestica had the lowest (P<0.0001) OMD. The addition of M to silage increased (P<0.0001) ME and OMD, as well as GP. However, the addition of U and the mixture of U and M reduced (P<0.0001) DMD, ME, OMD, SCFA, GY 24 and MCP. These results show that P. persica has the highest nutritive value and L. esculenta the lowest for ruminants. Additionally, the addition of M to forage from fodder trees increases rumen GP and fermentation, which may improve nutrient utilization in ruminants. | Abdelfattah Z M Salem ZHOU Chuan-she TAN Zhi-liang Miguel Mellado Moises Cipriano Salazar Mona M M Y Elghandopur Nicholas E Odongo | 2013 | Journal of Integrative Agriculture2013，12，7： | 3 |
| 3 | Frequent amplification of a chr19q13.41 microRNA polycistron in aggressive primitive neuroectodermal brain tumors显示文摘 | Li M Lee KF Lu Y Clarke I Shih D Eberhart C Collins VP Van Meter T Picard D Zhou L Boutros PC Modena P Liang ML Scherer SW Bouffet E Rutka JT Pomeroy SL Lau CC Taylor MD Gajjar A Dirks PB Hawkins CE Huang A. | 2009 | 中国神经肿瘤杂志2009，7，4： | 3 |
| 4 | Genetic transformation of wheat mediated by Agrobacterium tumefaciens显示文摘 | Cheng M Fry J E Pang S Zhou H Hironaka C M Duncan D R Conner T W Wan Y | 1997 | Plant Physiol1997，，： | 2 |
| 5 | Localizatian and activation of substance P receptors in unmyelinated axons of rat glabrous skin显示文摘 | Carlton S M Zhou S Coggeshall R E | 1996 | Brain Res1996，734，12： | 1 |
| 6 | Evaluation of phosphorus bioavailability in sediments of three contrasting lakes in China and the UK显示文摘 | Zhou Q X Gibson C E Zhu Y M | 2001 | Chemosphere2001，42，： | 1 |
| 7 | Nanodeliv- ery of MRI contrast agent enhances sensitivity of de- tection of lung cancer metastases显示文摘 | Freedman M Chang E H Zhou Q | 2009 | Acad Radiol2009，16，5： | 1 |
| 8 | An Investigation of Surface Damage in the High Speed Turning of In- conel 718 with Use of Whisker Reinforced Ceramic Tools显示文摘 | Zhou J M Bushlya V Stahl J E | 2012 | Journal of Materials Processing Tcchnol ogy2012，212，2： | 1 |
| 9 | Hybrid approach to complex thoracic aortic aneurysms in bighrisk patients: Surgical challenges and clinical outcomes显示文摘 | Zhou W Reardon M Peden E K | 2006 | J Vasc Surg2006，44，： | 1 |
| 10 | Development of sulfonamide AKT PH domain inhibitors 显示文摘 | Ahad A M Zhou L L Mash E A | 2011 | Bioorg Med Chem2011，19，6： | 1 |
| 11 | Telomerase reverse tran- scriptase promoter mutations in glandular lesions of the urinary bladder显示文摘 | Vail E Zheng X Zhou M | 2015 | Ann Diagn Pathol2015，19，5： | 1 |
| 12 | Generation of internal image monoclonal anti-idiotypic antibodies against idiotypic an- tibodies to GP5 antigen of porcine reproductive and respiratory syndrome virus 显示文摘 | Zhou E M Xiao Y Shi Y | 2008 | Journal of virological methods2008，149，2： | 1 |
| 13 | The degradation of apolipoprotein B100 is mediated by the ubiquitin-proteasome pathway and involves heat shock protein 70显示文摘 | Fisher E A Zhou M Mitchell D M | 1997 | The Journal of Biological Chemistry1997，272，20： | 1 |
| 14 | Molecular Weight Distribution of Hyperbranched Polymers Generated by Self-Condensing Vinyl Polymerization in Presence of a Multifunctional Initiator显示文摘 | Yan D Zhou Z M ü ller A H E | 1999 | Macromolecules1999，32，： | 1 |
| 15 | Universal cloning method by TA strategy显示文摘 | Zhou M Y Clerk S E Gonlez-Sanchez C E | 1995 | Biotechniques1995，19，： | 1 |
| 16 | Studying the vegetation response to simulated leakage of sequestered CO2 using spectral vegetation indices显示文摘 | Lakkaraju V R Zhou xiaobing Apple M E | 2010 | Ecological Informatics2010，5，5： | 1 |
| 17 | Glyphosate-tolerant CP4 and GOX genes as a selectable marker in wheat transformation显示文摘 | ZHOU H Arrowsmith J W Fromm M E | 1995 | Plant Cell Rep1995，，15： | 1 |
| 18 | Multilayer model of photon diffusion in skin显示文摘 | Schmitt J J M Zhou G X Walker E C | 1990 | J Opt Soc Am1990，7，： | 1 |
| 19 | Evaluation of phosphorus bioavailability in sediments of three contrasting lakes in China and the UK显示文摘 | Zhou Q X Gibson C E Zhu Y M | 2001 | Chemosphere2001，42，： | 1 |
| 20 | Genetic and experimental comparison of porcine circovirus type 2 (PCV2) isolates from cases with and without PCV2-associated lesions provides evidence for differences in virulence 显示文摘 | Opriessnig T McKeown N E Zhou E M | 2006 | Journal of General Virology2006，87，： | 1 |
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