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| 1 | Effect of Poria cocos Hydroethanolic Extract on Treating Adriamycin-Induced Rat Model of Nephrotic Syndrome显示文摘Objective: To evaluate the effect of Poria cocos(Schw.) Wolf hydroethanolic extract(PHE) against nephrotic syndrome(NS) in rats and to identify the potential active components from PHE. Methods: The high content compounds were isolated and purified by using column chromatography followed by preparative highperformance liquid chromatography(p-HPLC). Forty male Wistar rats with adriamycin(ADR)-induced NS were randomly divided into 5 groups, 8 in each group: model control group, positive control group(with prednisone treatment), PHE low-dose group, PHE middle-dose group and PHE high-dose group. Another 8 rats were recruited as vehicle control group. All rats received the intragastric administration of corresponding drugs or saline for 30 days. During the experimental period, rats' behavior and appearance were observed and recorded daily, and their body weights were recorded weekly. After treatment, 24-h urine samples were collected to evaluate the urine protein and urine creatinine(Ucr); then the rats were sacrificed to collect carotid blood and to determine the levels of serum total protein(TP), albumin(Alb), globulin(Glo), total cholesterol(TC) and cytokine interlukin-4(IL-4). Results: Six acidic components were isolated and identified from the PHE section: pachymic acid, 15α-hydroxydehydrotumulosic acid, trametenolic acid, dehydropachymic acid, 3β-hydroxy-lanosta-7,9(11), 24-trien-21-oic-acid and dehydroeburicoic acid. Compared with the model control group, the urine protein content were significantly decreased in the PHE treatment groups and positive control group(P<0.05), especially PHE middle-dose group(P<0.01). The Ucr values and serum levels of TP, Glo, TC and IL-4 in PHE low-and middle-dose groups were also presented obvious recover tendency as compared with the model control group(P<0.05 or P<0.01). However, positive control group and all PHE groups indicated no significant therapeutic effect on raising Alb value, although PHE low-and middle-dose treatment groups showed better outcomes than positive control group(P>0.05). Conclusions: PHE showed an encouraging therapeutic effect against ADR-induced NS in a rat model. PHE might be a group of effective substances for the treatment of NS. | ZAN Jun-feng SHEN Chan-juan ZHANG Li-ping LIU Yan-wen | 2017 | Chinese Journal of Integrative Medicine2017，23，12： | 15 |
| 2 | Bufalin suppresses the migration and invasion of prostate cancer cells through HOTAIR, the sponge of miR-520b显示文摘Bufalin, the major active component of the traditional Chinese medicine ChanSu obtained from the skin and parotid venom glands of toads, has long been known as an anticancer agent. Recent studies show that microRNAs (miRs) are involved in the anticancer activities of bufalin, while long non-coding RNAs (lncRNAs) are known to interact with miRNAs to regulate various biological functions. In this paper, we investigated the possible network related to the antimetastatic effect of bufalin in prostate cancer (PCa) cells. We demonstrated that bufalin (0.05?10 μM) dose-dependently suppressed the proliferation of prostate cancer DU145 and PC3 cells with IC50 values of 0.89 and 1.28 μM, respectively. Furthermore, bufalin treatment significantly suppressed the cell migration and invasion. To explore the role of lncRNAs in the antimetastatic activity of bufalin, we used an lncRNA microarray and found that HOX transcript antisense RNA (HOTAIR) was the most markedly downregulated lncRNA in bufalin-treated PCa cells. Overexpression of HOTAIR counteracted the suppressing effects of bufalin on DU145 and PC3 cells. We then predicted and verified that HOTAIR upregulated FGFR1 expression by sponging miR-520b in PCa cells. In 40 patients with PCa bone metastasis, we used in situ hybridization or immunohistochemical assay to assess the HOTAIR and FGFR1 expression, which revealed that both HOTAIR and FGFR1 expression were significantly higher in bone metastasis tissues than in the primary PCa tissues. In addition, the level of serum HOTAIR was positively associated with the levels of serum bone metabolic markers (CTx, OST, B-ALP and PINP) and may serve as a reasonable biomarker for PCa bone metastasis. Taken together, this is the first study revealing that HOTAIR promotes PCa bone metastasis, and bufalin may be a promising candidate for the treatment of this disease. | Jian-jun Zhang Xiao-hui Zhou Yan Zhou Yong-gang Wang Bin-zhi Qian Ai-na He Zan Shen Hai-yan Hu Yang Yao | 2019 | Acta Pharmacologica Sinica2019，40，9： | 13 |
| 3 | Pirarubicin inhibits multidrug-resistant osteosarcoma cell proliferation through induction of G~/M phase cell cycle arrest显示文摘 | Shui-er ZHENG Sang XIONG Feng LIN Guang-lei QIAO Tao FENG Zan SHEN Da-liu MIN Chun-ling ZHANG Yang YAO | 2012 | Acta Pharmacologica Sinica2012，33，6： | 9 |
| 4 | Existence of Solutions of Boundary Value Problem Differential a Nonlinear Three-Point for Third-Order Ordinary Equations显示文摘In this paper,existence of solutions of third-order differential equation ■with nonlinear three-point boundary condition ■is obtained by embedding Leray-Schauder degree theory in upper and lower solutions method,where a,b,c ∈ R,a < b < c;f :[a,c]×R3 → R,g :R3 → R,h :R2 → R and I :R3 → R are continuous functions.The existence result is obtained by defining the suitable upper and lower solutions and introducing an appropriate auxiliary boundary value problem.As an application,an example with an explicit solution is given to demonstrate the validity of the results in this paper. | SHEN Jian He ZHOU Zhe Yan YU Zan Pin | 2009 | Journal of Mathematical Research and Exposition2009，29，1： | 6 |
| 5 | Cancer gene therapy targeting angiogenesis:An updated review显示文摘Since the relationship between angiogenesis and tumor growth was established by Folkman in 1971, scientists have made efforts exploring the possibilities in treating cancer by targeting angiogenesis. Inhibition of angiogenesis growth factors and administration of angiogenesis inhibitors are the basics of anti- angiogenesis therapy. Transfer of anti-angiogenesis genes has received attention recently not only because of the advancement of recombinant vectors, but also because of the localized and sustained expression of therapeutic gene product inside the tumor after gene transfer. This review provides the up-to-date information about the strategies and the vectors studied in the field of anti-angiogenesis cancer gene therapy. | Ching-Chiu Liu Zan Shen Hsiang-Fu Kung Marie CM Lin | 2006 | World Journal of Gastroenterology2006，12，43： | 4 |
| 6 | 骨转换标志物在非小细胞肺癌骨转移临床应用价值的研究(英文)显示文摘Objective:The purpose of this study was to assess the clinical application value of bone turnover markers in non-small-cell lung cancer(NSCLC) patients with bone metastases.Including diagnosing bone metastases,detecting bone metastatic spread.Methods:Alkaline phosphatase(AKP),β-C-terminal telopeptide of type I collagen(β-CTx),osteocalcin(OST) and bone alkaline phosphatase(BALP) were measured in 76 patients with bone metastases from NSCLC and 44 normal people.Results:The level of AKP,β-CTx and BALP in patients with bone metastasis was significantly higher than in the normal people.Significant correlation was observed among bone turnover markers.The levels of BALP and OST were significantly correlated with the extent of bone metastasis.The patients with high-level CTx and low-level BALP had higher risk of pathologic fracture.Conclusion:In NSCLC patients with bone metastases,bone turnover markers can help to make diagnosis and evaluate the severity.It will have a wide range of use in clinical practice. | Zhiyu Wang Chen Yang Yumei Yang Zan Shen Hui Zhao Yang Yao | 2011 | The Chinese-German Journal of Clinical Oncology2011，10，2： | 3 |
| 7 | Brain-derived neurotrophic factor genes transfect rat bone marrow mesenchymal stem cells based on cationic polymer vector显示文摘BACKGROUND: Gene therapy is an effective expression of genes within target cells after transferring exogenous target genes. Both vector selection and transfection method are important factors for gene transfection. An ideal gene vector is required for a high transfusion of target gene and an exact introduction of target gene into specific target cells so as to express gene products. OBJECTIVE: To study the expression of mRNA and protein after transfecting rat bone marrow mesenchymal stem cells (BMSCs) with brain-derived neurotrophic factor (BDNF) genes based on cationic polymer vector. DESIGN, TIME AND SETTING: A randomized, controlled in vitro study using gene engineering, performed at the Neurobiology Laboratory, Xuzhou Medical College between October 2007 and April 2008. MATERIALS: PcDNA3.1 BDNF was obtained from Youbiai Biotechnological Company, Beijing and cationic polymer vector used was the SofastTM gene transfection reagent that was made by Taiyangma Biotechnological Co., Ltd., Xiamen. METHODS: BMSCs extracted from six Sprague Dawley (SD) rats aged 1 month were isolated and cultured in vitro. Third passage BMSCs were inoculated on a 6-well culture plate at the density of 1×106 cells/L. At about 80% confluence, BMSCs were transfected with PcDNA3.1-BDNF (2 μg) combined with SofastTM gene transfection reagent (6 μg) (BDNF group) or with PcDNA3.1 (2 μg) combined with SofastTM gene transfection reagent (6 μg) (blank vector group). Cells that were not transfected with any reagents but still cultured under primary culture conditions were used as a non-transfection group. MAIN OUTCOME MEASURES: Enzyme linked immunosorbent assay was used to measure time efficiency of BMSC-secreted BDNF protein. Twenty-four hours after gene transfection, RT-PCR was used to detect expression of BDNF mRNA in the BMSCs. Immunohistochemistry was used to determine expression of BDNF protein in the BMSCs. RESULTS: BDNF protein expression was detected at day 1 after gene transfection, rapidly increased after 5–9 days and gradually increased after 11–15 days in the BDNF group; moreover, BDNF protein expression was higher than that in the non-transfection group and the blank vector group at different time points (P < 0.01). Additionally, BDNF mRNA expression in the BDNF group was higher than that in the blank vector group and the non-transfection group (P < 0.01). CONCLUSION: A cationic polymer vector can effectively mediate the BDNF gene to transfect BMSCs; genetically modified BMSCs can express BDNF protein effectively for a long term. | Zunsheng Zhang Kun Zan Yonghai Liu Xia Shen | 2009 | Neural Regeneration Research2009，4，1： | 2 |
| 8 | Effects of Channel Width on Electrical Characteristics of Polysilicon TFTs with Multiple Nanowire Channels 显示文摘 | Wu Yung-Chun Chang Ting-Chang Liu Po-Tsun Chen Chi- Shen Tu Chun-Hao Zan Hsiao-Wen Tai Ya-Hsiang Chang Chun-Yen | 2005 | Electron Devices IEEE Transactions on2005，52，10： | 1 |
| 9 | Med19 Promotes Osteosarcoma Growth and Metastasis and Associates with Prognosis显示文摘 | Wenxi Yu Zhang Zhichang Min Daliu Yang Qingcheng Du Xuefei Tang Lina Lin Feng Sun Yuanjue Zhao Hui Zheng Shuier He Aina Li Hongtao Yao Yang Zan Shen | 2014 | European Journal of Cancer2014，，： | 1 |
| 10 | Expres- sion of Ezrin correlates with lung metastasis in Chinese patients with osteosarcoma显示文摘 | SHEN Xu- dong SHEN Zan ZHENG Shui -er | 2009 | Clin Invest Med2009，32，2： | 1 |
| 11 | Combined inhibition of insulin-like growth factor-1 receptor enhancesthe effects of gefitinib in a human non-small cell lung cancer resistant cellline显示文摘 | Hui wei Qi Zan Shen Li hong Fan | 2011 | Experimental and Therapeutic Medicine2011，，6： | 1 |
| 12 | New insights on the age of the Mengyejing Formation in the Simao Basin,SE Tethyan domain and its geological implications显示文摘Knowledge on the origin and mechanism of the large potash formation in the southeast Tethyan tectonic domain are still under debate,one of which mainly due to the controversial ages of the potash-bearing strata.Here we present a detailed detrital zircon U-Pb geochronologic-magnetostratigraphic study of the potash-bearing Mengyejing(MYJ)Formation(Fm)from the Simao Basin.948 paleomagnetic cores and 4 detrital zircon U-Pb samples were collected from a 932-m thick Jiangcheng section.Thermal demagnetization isolated 857 primary characteristic remnant magnetization(ChRM)directions,indicated by positive reversals and fold tests and petrologic examinations.Seven normal and seven reversed polarity zones were identified and correlated well with chrons C27r to C34n of the geomagnetic polarity timescale(GPTS2012),yielding the first known age sequence of>112 to~63 Ma for the MYJ Fm.The ages for the potash bearing strata in the Simao commenced~20 Ma earlier than those in the adjacent Khorat basins,opposite to the traditional viewpoint that the former would rather correspond to the middle and lower parts of the latter.Given the paleogeographic reconstruction of likely a pan-Simao-Khorat basin during the Cretaceous,~85 Ma would be another important potential period for future potash exploration in the Simao Basin. | Maodu YAN Dawen ZHANG Xiaomin FANG Weilin ZHANG Chunhui SONG Chenglin LIU Jinbo ZAN Miaomiao SHEN | 2021 | Science China Earth Sciences2021，64，2： | 1 |
| 13 | Meta-analysis of docetaxel-based doublet versus docetaxel alone as second-line treatment for advanced non-small-cell lung cancer显示文摘 | Wei-Xiang Qi Zan Shen Yang Yao | 2012 | Cancer Chemotherapy and Pharmacology2012，，1： | 1 |
| 14 | 骨肉瘤血行转移与CXCR4、VEGF相关性研究(英文)显示文摘Objective:We determine whether chemokine receptor CXCR4 and vascular endothelial growth factor(VEGF) expression related to the metastasis and survival outcome of patients with osteosarcoma.Methods:Tissue microarray(TMA) was used to detect the expression of CXCR4 and VEGF in 56 osteosarcoma patient samples.Two-year follow-up was performed to observe the metastatic behavior and overall survival of osteosarcoma patients.Results:There was a significant correlation between the expression levels of CXCR4 and VEGF in 56 osteosarcoma patient samples(P = 0.002).Univariate analysis revealed the expression of CXCR4 and VEGF was not associated with age, gender and the level of ALP but associated with clinical stage.Conclusion:These data raises the possibility that VEGF could regulate the levels of CXCR4 to promote the migration of tumor cells to target organs.CXCR4 and VEGF expression are highly correlated with metastatic progression in patients with osteosarcoma and their immunohistochemical expression have predictive value for the metastatic development. | Feng Lin Shuier Zheng Zan Shen Lina Tang Ping Chen Yuanjue Sun Hui Zhao Yang Yao | 2009 | The Chinese-German Journal of Clinical Oncology2009，8，5： | 1 |
| 15 | The affordability of owner occupied housing in Beijing 显示文摘 | Zan Yang Yue Shen | 2008 | Journal of Housing and the Built Environment2008，23，4： | 1 |
| 16 | The Affordability of Owner Occupied Housing in Beijing 显示文摘 | Zan Yang Yue Shen | 2008 | Journal of Housing and the Built Environment2008，23，4： | 1 |
| 17 | Combined inhibition of insulin-like growth factor-1 receptor enhancesthe effects of gefitinib in a human non-small cell lung cancer resistant cellline显示文摘 | Hui wei Qi Zan Shen Li hong Fan | 2011 | Experimental and Therapeutic Medicine2011，，6： | 1 |
| 18 | Crowd Counting for Real Monitoring Scene显示文摘Crowd counting is a challenging task in computer vision as realistic scenes are al?ways filled with unfavourable factors such as severe occlusions, perspective distortions and di?verse distributions. Recent state-of-the-art methods based on convolutional neural network (CNN) weaken these factors via multi-scale feature fusion or optimal feature selection through a front switch-net. L2 regression is used to regress the density map of the crowd, which is known to lead to an average and blurry result, and affects the accuracy of crowd count and po?sition distribution. To tackle these problems, we take full advantage of the application of gen?erative adversarial networks (GANs) in image generation and propose a novel crowd counting model based on conditional GANs to predict high-quality density maps from crowd images. Furthermore, we innovatively put forward a new regularizer so as to help boost the accuracy of processing extremely crowded scenes. Extensive experiments on four major crowd counting datasets are conducted to demonstrate the better performance of the proposed approach com?pared with recent state-of-the-art methods. | LI Yiming LI Weihua SHEN Zan NI Bingbing | 2020 | ZTE Communications2020，18，2： | 0 |
| 19 | 中国儿童骨肉瘤与青少年骨肉瘤患者的相互比较分析(英文)显示文摘Objective:The aim of this study was to assess the differences in clinical appearance and prognosis of osteosarcoma between preadolescent and adolescent in Chinese patients and investigate whether age at diagnosis is a prognostic indicator.Methods:Between May 2002 and May 2009,ninety-six children with high-grade osteosarcoma treated at our institute were stratified according to the age of 10.There were 19 preadolescents(≤ 10 years) and 77 adolescents(10 years < age ≤ 19 years),and their medical records were reviewed and compared using Fisher exact tests.Overall survival and disease-free survival was estimated by Kaplan-Meier methods and compared using log-rank tests.The prognostic significance of the various factors on survival was examined by Cox regression analysis.Results:There were no significant differences in terms of Karnofsky Performance Status(KPS) score,Enneking stage,tumor location,histologic type,pathologic fracture,tumor necrosis rate,tumor size,relapse and frequencies of adjuvant chemotherapy between the two groups.A high proportion of preadolescent patients was treated with amputation(78.9% vs.44.2%;P = 0.01).The 5-year survival of the preadolescent and adolescent groups was(38 ± 14)% and(33 ± 10)%,and the 2-year disease free survival for each group was(45 ± 12)% and(44.4 ± 6.3)%,respectively.Our study showed that age did not have any statistical significance for survival(P = 0.803).Univariate analysis indicated that KPS score;frequencies of adjuvant chemotherapy,tumor size and relapse were significantly related to overall survival.Multivariate Cox regression analysis revealed that both frequencies of adjuvant chemotherapy and relapse were independently prognostic factors for survival.Conclusion:Clinical characteristics and survival between the preadolescents and adolescents with osteosarcoma in China were compatible.So we suggested that there did not need to treat preadolescents patients by alternative and/or aggressive therapies compared with adolescent patients. | Weixiang Qi Zan Shen Lina Tang Aina He Yumei Yang Feng Lin Yang Yao | 2012 | The Chinese-German Journal of Clinical Oncology2012，11，5： | 0 |
| 20 | Efficient production of 14α‑OH‑AD by engineered Mycolicibacterium neoaurum via coupled cofactor and reconstructed electron transport system显示文摘14α-hydroxy-androst-4-ene-3,17-dione(14α-OH-AD)is an important precursor for the synthesis of steroid drugs with anticancer and carcinolytic activity.Initially,14α-OH-AD was mostly synthesized by whole-cell fermentation of mold fungi using androstenedione(AD)as a substrate,which had difficulties in product isolation and purification as well as problems of high production cost.In this study,the source of the 14α-hydroxylase gene was expanded.And 14α-hydroxylase genes were heterologously expressed in Mycolicibacterium neoaurum(MNR)M3ΔksdD,which enabled the one-step biotransformation from the cheap substrate phytosterols(PS)to 14α-OH-AD,reducing the difficulty of product purification and production cost.What is more,to alleviate the problem of poor activity of 14α-hydroxylase,the 14α-hydroxylase gene was co-expressed with the electron transport chain element genes and the coenzyme regeneration genes,and a superior engineered strain MNR M3ΔksdD/pMV261-14α-G6PDH was obtained.Finally,the transformation conditions were optimized for the transformation of PS by the engineered strain.The molar yield of 14α-OH-AD reached to 60.4±2.3%(about 0.22 g/L productivity).This study investigated for the first time the effects of the tandem electron transport chain element genes and the tandem coenzyme regeneration genes on the 14α-hydroxylation reaction,providing a theoretical basis for the industrial production of 14α-OH-AD. | Chenwei Zhang Yanbing Shen Yuanyuan Gao Zehui Zan Min Wang | 2023 | Systems Microbiology and Biomanufacturing2023，3，2： | 0 |
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