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您的检索式：作者名="ZHUHuabing"
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| 1 | Production of transgenic calves by somatic cell nuclear transfer显示文摘Bovine fetal oviduct epithelial cells were transfected with constructed double marker selective vector(pCE-EGFP-IRES-Neo-dNdB) containing the enhanced green fluorescent protein (EGFP) and neomycin-resistant(Neo^r) genes by electroporation, and a transgenic cell line was obtained. Somatic cell nuclear transfer (SCNT) was cartied out using the transgenic cells as nuclei donor. A total of 424 SCNT embryos were reconstructed and 208 (49.1%) of them developed to blastocyst stage. 17 blastocysts on D 7 after reconstruction were transferred to 17 surrogate calves,and 5 (29.4%) recipients were found to be pregnant. Three of them maintained to term and delivered three cloned calves.PCR and Southern blot analysis confirmed the integration of transgene in all of the three cloned calves. In addition, expression of EGFP was detected in biopsy isolated from the transgenic cloned calves and fibroblasts derived from the biopsy. Our results suggest that transgenic calves could be efficiently produced by SCNT using transgenic cells as nuclei donor. Furthermore, all cloned animals could be ensured to be transgenic by efficiently pre-screening transgenic cells and SCNT embryos using the constructed double marker selective vector. | GONGGuochun DAIYunping FANBaoliang ZHUHuabing WANGLili WANGHaiping TANGBo LIUYing LIRong WANGRong HUANGYinghua LINing | 2004 | Chinese Science Bulletin2004，49，2： | 7 |
| 2 | Research on the computer monitoring and control system of diesel motor显示文摘The working conditions of diesel motors used in somespecial condition are complex. By using microcomputer control,A/D conversion and digital tele-communication techniques , au-tocontrol of the working process , digital sampling of parameters,tele-communication concentrative monitoring and control are real-ized . When the signal sampled goes beyond the limit, the sta-tion alarms, reacts automatically and stops automatically if neces-sary. | CaiFuqing ZhuHuabing WangShuzong | 2003 | 微计算机信息2003，19，6： | 0 |
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