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| 1 | XA23 Is an Executor R Protein and Confers Broad-Spectrum Disease Resistance in Rice显示文摘 | Chunlian Wang Xiaoping Zhang Yinglun Fan Ying Gao Qinlong Zhu Chongke Zheng Tengfei Qin Yanqiang Li Jinying Che Mingwei Zhang Bing Yang Yaoguang Liu Kaijun Zhao | 2015 | Molecular Plant2015，8，2： | 55 |
| 2 | HrcQ is necessary for Xanthomonas oryzae pv. oryzae HR-induction in non-host tobacco and pathogenicity in host rice显示文摘Bacterial blight, caused by Xanthomonas oryzae pv. oryzae(Xoo), is one of the most destructive diseases of rice(Oryza sativa L.) worldwide. The type III secretion system(T3SS) of Xoo, encoded by the hrp(hypersensitive response and pathogenicity) genes, plays critical roles in conferring pathogenicity in host rice and triggering a hypersensitive response(HR) in non-host plants. To investigate the major genes conferring the pathogenicity and avirulence of Xoo, we previously constructed a random Tn5-insertion mutant library of Xoo strain PXO99A. We report here the isolation and characterization of a Tn5-insertion mutant PXM69. Tn5-insertion mutants were screened on indica rice JG30, which is highly susceptible to PXO99A, by leaf-cutting inoculation.Four mutants with reduced virulence were obtained after two rounds of screening. Among them, the mutant PXM69 had completely lost virulence to the rice host and ability to elicit HR in non-host tobacco. Southern blotting analysis showed a single copy of a Tn5-insertion in the genome of PXM69. PCR walking and sequencing analysis revealed that the Tn5 transposon was inserted at nucleotide position 70,192–70,201 in the genome of PXO99A, disrupting the type III hrc(hrp-conserved) gene hrcQ, the first gene in the D operon of the hrp cluster in Xoo. To confirm the relationship between the Tn5-insertion and the avirulence phenotype of PXM69, we used the marker exchange mutagenesis to create a PXO99Amutant, ΔhrcQ::KAN, in which the hrcQ was disrupted by a kanamycin-encoding gene cassette at the same site as that of the Tn5-insertion. ΔhrcQ::KAN showed the same phenotype as mutant PXM69. Reintroduction of the wild-type hrcQ gene partially complemented the pathogenic function of PXM69. RT-PCR and cellulase secretion assays showed that the Tn5-disruption of hrcQ did not affect transcription of downstream genes in the D operon and function of the type II secretion system. Our results provide new insights into the pathogenic functions of clustered hrp genes in Xoo. | Xiaoping Zhang Chunlian Wang Chongke Zheng Jinying Che Yanqiang Li Kaijun Zhao | 2013 | The Crop Journal2013，1，2： | 4 |
| 3 | RNAi-mediated suppression of the abscisic acid catabolism gene Os ABA8ox1 increases abscisic acid content and tolerance to saline–alkaline stress in rice(Oryza sativa L.)显示文摘Saline–alkaline(SA) stress is characterized by high salinity and high alkalinity(high p H), which severely inhibit plant growth and cause huge losses in crop yields worldwide. Here we show that a moderate elevation of endogenous abscisic acid(ABA) levels by RNAi-mediated suppression of Os ABA8 ox1(Os ABA8 ox1-kd), a key ABA catabolic gene, significantly increased tolerance to SA stress in rice plants. We produced Os ABA8 ox1-kd lines in two different japonica cultivars, Dongdao 4 and Nipponbare. Compared with nontransgenic control plants(WT), the Os ABA8 ox1-kd seedlings accumulated 25.9%–55.7% higher levels of endogenous ABA and exhibited reduced plasmalemma injury, ROS accumulation and Na;/K;ratio, and higher survival rates, under hydroponic alkaline conditions simulated by 10, 15, and 20 mmol L-1 of Na;CO;. In pot trials using SA field soils of different alkali levels(p H 7.59, 8.86, and 9.29), Os ABA8 ox1–kd plants showed markedly higher seedling survival rates and more vigorous plant growth, resulting in significantly higher yield components including panicle number(85.7%–128.6%), spikelets per panicle(36.9%–61.9%), branches(153.9%–236.7%), 1000–kernel weight(20.0%–28.6%), and percentage of filled spikelets(96.6%–1340.8%) at harvest time. Under severe SA soil conditions(p H = 9.29, EC = 834.4 μS cm-1),Os ABA8 ox1-kd lines showed an 194.5%–1090.8% increase in grain yield per plant relative to WT plants.These results suggest that suppression of Os ABA8 ox1 to increase endogenous ABA levels provides a new molecular approach for improving rice yield in SA paddies. | Xiaolong Liu Xianzhi Xie Chongke Zheng Lixing Wei Xiaowei Li Yangyang Jin Guohui Zhang Chang-Jie Jiang Zhengwei Liang | 2022 | The Crop Journal2022，10，2： | 3 |
| 4 | High-resolution genetic mapping of rice bacterial blight resistance gene Xa23显示文摘 | Chunlian Wang Yinglun Fan Chongke Zheng Tengfei Qin Xiaoping Zhang Kaijun Zhao | 2014 | Molecular Genetics and Genomics2014，，5： | 3 |
| 5 | OsSRK1,an Atypical S-Receptor-Like Kinase Positively Regulates Leaf Width and Salt Tolerance in Rice显示文摘Receptor-like kinases(RLKs)are important for plant growth,development and defense responses.The S-receptor protein kinases(SRKs),which represent an RLK subfamily,control the selfincompatibility among Brassica species.However,little information is available regarding SRK functions in rice.We identified a gene OsSRK1 encoding an atypical SRK.The transcript of OsSRK1 was induced by abscisic acid(ABA),salt and polyethylene glycol.OsSRK1 localized to the plasma membrane and cytoplasm.Leaf width was increased in OsSRK1-overexpression(OsSRK1-OX)transgenic rice plants,likely because of an increase in cell number per leaf.Furthermore,the expression levels of OsCYCA3-1 and OsCYCD2-1,which encode positive regulators of cell division,were up-regulated in leaf primordium of OsSRK1-OX rice plants relative to those in wild type.Meanwhile,the expression level of OsKRP1,which encodes cell cycle inhibitor,was down-regulated in the OsSRK1-OX plants.Therefore,it is deduced that OsSRK1 regulates leaf width by promoting cell division in the leaf primordium.Additionally,OsSRK1-OX plants exhibited enhanced ABA sensitivity and salt tolerance compared with wild type.These results suggest that OsSRK1 plays important roles in leaf development and salt responses in rice. | ZHOU Jinjun JU Peina ZHANG Fang ZHENG Chongke BAI Bo LI Yaping WANG Haifeng CHEN Fan XIE Xianzhi | 2020 | Rice science2020，27，2： | 2 |
| 6 | mSwap: a large-scale image-compositing method with optimal m-ary tree显示文摘With the increasing of computing ability,large-scale simulations have been generating massive amounts of data in aerodynamics.Sort-last parallel rendering is the most classical image compositing method for large-scale scientific visualization.However,in the stage of image compositing,the sort-last method may suffer from scalability problem on large-scale processors.Existing image compositing algorithms tend to perform well in certain situations.For instance,Direct Send is well on small and medium scale;Radix-k gets well performance only when the k-value is appropriate and so on.In this paper,we propose a novel method named mSwap for scientific visualization in aerodynamics,which uses the best scale of processors to make sure its performance at the best.mSwap groups the processors that we can use with a(m,k)table,which records the best combination of m(the number of processors in subgroup of each group)and k(the number of processors in each group).Then in each group,using a m-ary tree to composite the image for reducing the communication of processors.Finally,the image is composited between different groups to generate the final image.The performance and scalability of our mSwap method is demonstrated through experiments with thousands of processors. | Min Hou Chongke Bi Fang Wang Liang Deng Gang Zheng Xiangfei Meng | 2021 | Advances in Aerodynamics2021，3，1： | 0 |
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