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| 1 | Ultrasound-associated extraction of seed oil of Korean pine显示文摘Experiment on ultrasound- associated extraction of seed oil of Korean pine (Pinus koraiensis) was conducted in Northeast Forestry University, Harbin, China. The factors affecting extraction yield, such as ultrasonic frequency, extracting temperature, extracting time and the ratio of material to liquid (ratio of Korean pine seed to absolute alcohol), were analyzed under specific condition and the optimal extracting parameters were obtained as the ultrasonic frequency 32 000 Hz, the extracting temperature 80℃, the extracting time 50 rain, and the ratio of material to liquid 1: 30. The study demonstrates that ultrasound is a reliable and great efficiency tool for the fast extraction of Korean pine seed oil。 | ZHANGYing WANGZhen-yu CHENXiao-qiang | 2005 | Journal of Forestry Research2005，16，2： | 36 |
| 2 | N-AcetyI-L-cysteine and pyrrolidine dithiocarbamate inhibited nuclear factor-κB activation in alveolar macrophages by different mechanisms显示文摘Aim:To study the effects of N-acetyl-L-cysteine(NAC)and pyrrolidine dithio-carbamate(PDTC)on the phosphorylation of IκB kinase(IKK)β,IKKα,and IλBαin alveolar macrophages(AM),and to explore the pharmacological mechanisms ofNAC and PDTC as inhibitors of NF-κB activation.Methods:AM were collectedfrom bronchoalveolar lavage fluid from the patients with chronic obstructive pul-monary disease.The AM were incubated for 1.5 h with NAC and PDTC,and thenstimulated for 90 min by either tumor necrosis factor(TNF)-α or interleukin(IL)-1.Western blotting was used to detect the protein phosphorylation levels of IKKβ,IKKα,and IκBα.NF-κB activity was analyzed by using an electrophoretic mobil-ity shift assay.Results:NAC inhibited the phosphorylation of IKKβ,IKKα,andIκBα induced by TNF-α,but had no effect on the phosphorylation of IKKβ,IKKαand IκBα induced by IL-1.PDTC did not inhibit the phosphorylation of IκBαinduced by TNF-α or IL-1.Similarly,NAC inhibited the activation of NF-κBinduced by TNF-α,but had no effect on the activation of NF-κB induced by IL-1.PDTC significantly inhibited the activation of NF-κB induced by TNF-α and IL-1.The electrophoretic mobility shift assay also showed that PDTC and NAC do notdirectly inhibit NF-κB DNA binding activity in vitro.Conclusion:PDTC preventsthe degradation of IκBα via the ubiquitylation-proteasome proteolytic pathway.NAC can inhibit the processes upstream of IKK activation induced by TNF-α,which results in the decline of NF-κB activity. | Ya-qing LI Zhen-xiang ZHANGI Yong-jian XU Wang NI Shi-xin CHEN Zhao YANG Dan MA | 2006 | Acta Pharmacologica Sinica2006，27，3： | 29 |
| 3 | A Genetic Map Constructed Using a Doubled Haploid Population Derived from Two Elite Chinese Common Wheat Varieties显示文摘Genetic mapping provides a powerful tool for the analysis of quantitative trait loci (QTLs) at the genomic level. Herein, we report a new genetic linkage map developed from an F1-derived doubled haploid (DH) population of 168 lines, which was generated from the cross between two elite Chinese common wheat (Triticum aestivum L.) varieties, Huapei 3 and Yumai 57. The map contained 305 loci, represented by 283 simple sequence repeat (SSR) and 22 expressed sequence tag (EST)-SSR markers, which covered a total length of 2141.7 cM with an average distance of 7.02 cM between adjacent markers on the map. The chromosomal locations and map positions of 22 new SSR markers were determined, and were found to distribute on 14 linkage groups. Twenty SSR loci showed different chromosomal locations from those reported in other maps. Therefore, this map offers new information on the SSR markers of wheat. This genetic map provides new opportunities to detect and map QTLs controlling agronomically important traits. The unique features of this map are discussed. | Kun-Pu Zhangi Liang Zhao Ji-Chun Tian Guang-Feng Chen Xiao-Ling Jiang Bin Liu | 2008 | Journal of Integrative Plant Biology2008，50，8： | 10 |
| 4 | Inhibiting the expression of CD28 costimulatory molecule on human lymphocytes by special siRNA显示文摘Background The B7/CD28 pathway provides critical costimulatory signals for complete T cell activation, and members of this pathway have served as useful targets for immunotherapeutic strategies. In this study, we investigated the RNA interference (RNAi) effect induced by small interfering RNA (siRNA) targeting CD28 mRNA on human lymphocytes and its specificity.Methods According to CD28 gene sequence, we designed and synthysized three different siRNAs ( siRNA-1,siRNA-2, siRNA-3 ) containing 21 bases using SilencerTM siRNA construction kit. These siRNAs were transfected into freshly isolated human lymphocytes with Lipofectamine 2000 reagent. At 24-hour, 48-hour and 72-hour post transfection, these cells were collected and analyzed. The changes of surface expression of CD28 gene were detected by flow cytometry, and the changes of CD28 mRNA levels were determined by semiquantitative reverse transcription polymerase chain reaction (RT-PCR). The cell viability of transfected lymphocytes was determined by methyl thiazolyl tetrazolium (MTT) assay and trypan blue dye exclusion assay.Results Three siRNAs (siRNA-1, siRNA-2, siRNA-3) specifically targeting CD28 mRNA were successfully designed and constructed. Flow cytometry analysis showed that a decrease in CD28 expression was detectable at 24-hour post transfection. Different siRNA showed different inhibition effects on CD28 expression. At 48-hour post transfection, the degrees of reduction with siRNA-1, siRNA-2 and siRNA-3 were 22. 10% ± 1.63% ,73.50% ± 1.02% and 42.90% ± 0.89% respectively compared with the control ( P < 0. 001 ). Neither of the groups transfected only with siRNA or lipo showed marked reduction in CD28 expression (3.15% ± 0.75% and 4. 55% ±0. 80% ) (P >0. 05). Moreover, lymphocytes treated with siRNA-co showed no marked reduction in CD28 expression (5.07% ± 0. 96% ) (P > 0. 05 ). The results of semi-quantitative RT-PCR assay indicated CD28 mRNA level was inhibited after transfection of specific siRNAs. At least 4-fold of reduction in siRNA-2 group occurred at 48-hour post transfection compared with the control (P < 0. 001 ). MTT assay and trypan blue dye exclusion assay demonstrated that the viable cell rations of transfected lymphocytes were significantly reduced in siRNA-1, siRNA-2 and siRNA-3 groups at 48-hour post transfection (P <0.01 ). The control groups showed no marked reduction in cell viability ( P > 0.05 ).Conclusions Three different siRNAs were synthesized and transfected into lymphocytes. They could reduce the expression of CD28 and the CD28 mRNA level, siRNA-2 was the most efficient. The cell viability reduced correspondingly. Therefore, the silencing effect on CD28 mRNA induced by siRNA may contribute to costimulatory blockade. This result show that siRNA may be useful for further study on graft-versus-host disease (GVHD) after allogeneic bone marrow transplantation (allo-BMT). | XUKai-lin ZHANGYing PANXiu-ying LUQun-xian | 2005 | Chinese Medical Journal2005，，6： | 8 |
| 5 | Synthesis of a new meso/microporous composite molecular sieve of MCM-41/mordenite显示文摘An MCM-41/mordenite composite with two- fold porous structure and stepwise-distributed acidity was prepared for the first time by using zeolite mordenite as the silica-alumina source. The composite molecular sieve has been investigated and compared with a mechanical mixture of mordenite and MCM-41 for their structure, acidity and catalytic activity by means of XRD, N2 adsorption and desorption, HRTEM, DTG, NH3-TPD and catalytic reaction. The characterization results show that the structure and property of the composite molecular sieve are quite different from those of the mechanical mixture, which might be ascribed to the incorporation of secondary building units characteristic of zeolite mordenite into the mesoporous walls of the composite which gives rise to the thicker mesoporous walls, the higher hydrothermal stability and more strong acid sites. Furthermore, the new strategy could be used as a new general method for the preparation of catalysts for the reaction system with multifold large molecules, and the results were well confirmed by the dealkylation of C10+ aromatic hydrocarbon. | WANGShan DOUTao LIYuping ZHANGYing YANZichun LIXiaofeng | 2005 | Chinese Science Bulletin2005，50，12： | 6 |
| 6 | Research on the magnetic material of Sm-Fe matrix nitrides显示文摘In this paper,the types of Sm-Fe matrix compounds and their correlations are introduced,and progress of research on the magnetic materials of Sm-Fe matrix nitrides is also reviewed.Possible research trends of future permanent magnetic materials of Sm-Fe matrix nitrides are briefly predicted. | CUIChunxiang SUNJibing ZHANGYing WANGRu LILin LIANGZhimei | 2005 | Progress in Natural Science:Materials International2005，15，3： | 5 |
| 7 | Correlation of Twist upregulation and senescence bypass during the progression and metastasis of cervical cancer显示文摘颈的癌为本地侵略和淋巴节点转移与高倾向被联系。然而,驾驶颈的癌症的前进和转移的分子的改变仍然保持不清楚。细胞的老朽作为机制被建议了保护一个有机体免于癌症前进和转移。另外,扭动,一个基本 helix-loop-helix 抄写因素,因为它是在人的癌症的许多类型的 overexpressed,作为 oncogene 被建议了。这基因也在调整侵略和转移展出积极功能。在这研究,扭曲强烈并且断然在正常织物,有鳞的房间癌(SCC ) IAIIA,和 SCC IIBIIIB 被表示(4.3% , 44% ,和 88.9% ,分别地) 。老朽标记 CBX3 的强壮的积极表情分别地是 39.1% , 32% ,和 15.6% 。在 SCC 组与或没有淋巴节点转移的扭曲的强壮的积极表情是 80.8% 和 50% 。为 CBX3,如此的表情分别地是 7.7% 和 29.5% 。结果也证明扭曲的表示相反地与 CBX3 的被相关。而且,有在 SiHa 的目标 siRNA 的扭曲击倒触发了细胞的老朽 CBX3 和联系老朽的牛乳糖活动的染色质标记的正式就职。我们的结果建议扭曲的表示在颈的癌症的前进和转移期间增加了。而且,导致扭曲的老朽绕过在这个过程是重要的。 | Tian Wang Yan Li Abidan Tuerhanjiang Wenwen Wang Zhangying Wu Ming Yuan Shixuan Wang | 2014 | Frontiers of Medicine2014，8，1： | 4 |
| 8 | Estimation of stature by using the dimensions of the right hand and right foot in Han Chinese adults显示文摘The Han Chinese people are the main ethnic group in China and the largest ethnic group in the world.The dimensions of the hands and feet have been successfully used for the estimation of stature.A total of 26,927 healthy adult subjects,comprising 13,221 men and 13,706 women,were recruited.The survey samples were chosen through random cluster sampling.The mean values were significantly higher in men than those in women for all measurements(P<0.001).All the measurements showed a statistically significant correlation with stature(P<0.01).The combination of the right hand length and the right foot length was the best predictor of stature because it had the lowest standard error of estimate.The use of multiple regression equations yielded better results than did the use of linear regression equations.The accuracy of stature prediction ranged from ±4.81 to ±6.39 cm.The present study was of great importance with regards to improving the physical anthropology database of ethnic groups in China. | Xinghua Zhangi Yu Wei Lianbin Zhengi Keli Yu Dapeng Zhao Jinping Bao Yonglan Li Shunhua Lu Huanjiu Xi Guochang Xu Youfeng Wen | 2017 | Science China(Life Sciences)2017，60，1： | 4 |
| 9 | Recent advances in application of moving bed bioreactors for wastewater treatment from recirculating aquaculture systems: A review显示文摘In recent years,government investments in implementing restrictive public policies on the treatment and discharge of effluents from the aquaculture industry have increased.Hence,efficient and cleaner methods for aquaculture production are needed.Recirculating aquaculture systems(RAS)offers water conservation by recycling the treated aquaculture water for reuse.RAS wastewater treatment using a moving bed bioreactors(MBBRs)process has been considered well suited for maintaining good water quality,thereby making fish farming more sustainable.Currently,improvements were achieved in tackling the influence of salinity,organic matter,disinfectant,and bioreactor start-up process on the MBBR performance efficiency.This review highlights an updated overview of recent development made using MBBR to treat the residual water from RAS.Precisely,nitrification and simultaneous nitrification-denitrification(SND),and other hybrid processes for nitrogen removal were elucidated.Finally,future challenges and prospects of MBBRs in RAS facilities that need to be considered were also proposed. | Abubakar Shitu Gang Liu Aliyu Idris Muhammad Yadong Zhang Musa Abubakar Tadda Wanhe Qi Dezhao Liu Zhangying Ye Songming Zhu | 2022 | Aquaculture and Fisheries2022，7，3： | 3 |
| 10 | Comparative Study of Carbon Storage and Allocation Characteristics of Mature Evergreen Broad-leaved Forest显示文摘Evergreen broad-leaved forest is an important forest type in China.This paper analyzes the allocation characteristics of vegetation and soil carbon pool of evergreen broad-leaved forest,to understand the current status of research on the carbon storage of evergreen broadleaved forest as well as shortcomings.In the context of global climate change,it is necessary to carry out the long-term research of evergreen broad-leaved forest,in order to grasp the formation mechanism of evergreen broad-leaved forest productivity,and the impact of climate change on the carbon sequestration function of evergreen broad-leaved forest ecosystem. | Zhangquan ZENG Canming ZHANGY Yandong NIU Xiquan LI Zijian WU Jia LUO | 2014 | Asian Agricultural Research2014，6，7： | 3 |
| 11 | Development of Exterior Anti-corrosion Coating Production Line for Large Diameter Hot Bent Pipes显示文摘The epoxy powder exterior anti-corrosion coating production line for bent pipes with a single (double) course production is a technologically advanced bent pipe anti corrosion method with cost efficiency, environment friendliness and stable coating quality. The quality of the coating on the bent pipe fully meets the requirements of the current national and industrial standards. The application of the technology has filled the gap in the bent pipe anti corrosion coating area of China, and leads the world technologically. With this technology the coating quality of the bent pipe has greatly improved, resulting in significant social and economic benefits. With the use of the technology in various large scale pipeline projects such as the 'West to East Gas Pipeline Project', it will exhibite a greater potential in the future pipeline projects with a broad application prospect. | JiaoRuyi ZhangYing | 2004 | 工程科学(英文版)2004，2，4： | 3 |
| 12 | An Analysis and Design of the Virtual Simulation Software Based on Pattern显示文摘The paper makes a detailed analysis and design of the Vega application software based on Windows NT platform. It includes object oriented software analysis and design, design patterns and Windows kernel mechanism. The paper brings forward a design pattern, a fence pattern, and depends on this pattern. Windows NT memory mapped files adopted, the paper presents a Vega application solution based on the multi process technique. Although the design solution is developing under a real time simulation system, it is established at the clear analysis of the Vega system, therefore, the solution has extensive practicability and many uses. | ZHANGYing SUNZhi-xin 等 | 2002 | The Journal of China Universities of Posts and Telecommunications2002，9，2： | 3 |
| 13 | Expert Consensus on Nurses'Human Caring for COVID-19 Patients in Different Sites显示文摘Summary:This study aims to develop the expert consensus on nurse's human caring for Corona Virus Disease 2019(COVID-19)patients in different sites,and thus provide a guideline on providing whole process and systematic caring for COVID-19 patients.Based on the frontline experiences of human caring for COVID-19 patients and the review of literature,the initial draft of consensus was made and finalized after online meeting and revisions.The experts reached consensus on the following parts:terms and definitions,principles of human caring for COVID-19 patients,and human caring measures for COVID-19 patients in different sites.The expert consensus is practical,concise,and reasonable for guiding the nurses providing human caring for COVID-19 patients,as well as other similar infectious diseases. | Feng-jian ZHANGI De-ying HU Yi-lan LIU Hong LI Xiao ping ZHU Shao-shan PAN | 2020 | Current Medical Science2020，40，4： | 2 |
| 14 | Transferrin receptor and Fc α/μ receptor may not be the major IgA_1 receptor on human mesangial cells显示文摘IgA nephropathy (IgAN) is the most common form of primary glomerulonephritis. 1 The histopathology of IgAN is characterized by abundance of mesangial matrix and proliferation of mesangial cells. IgA1 deposition in the mesangium plays an important role in the inflammatory process in this disease. | HURui-hai ZHANGYing ZHAOMing-hui | 2005 | Chinese Medical Journal2005，，9： | 2 |
| 15 | The Feature of DDR SDRAM and the Implementation of DDR SDRAM Controllers via VHDL显示文摘Double Data Rate Synchronously Dynamic RAM(DDR SDRAM ) is a kind of new high capacity and high speed ram chip. The article describes its working features. Implementation of the DDR SDRAM controller is analyzed and some details are also given. | GUOLi ZHANGYing | 2002 | The Journal of China Universities of Posts and Telecommunications2002，9，1： | 2 |
| 16 | Studies of Composition and Structure of CO_2 Corrosion Film of X56 Steel in the Medium of CO_2 and Salt Water显示文摘The X56 steel samples was corroded in the medium of salt water solution at the conditions of CO2 partial pressure POM 0.5 to 2.0 MPa, temperature 80 °C and flow rate 1.4m/s. Corrosion weigh loss, composition and structure, morphology and phase of corrosion films of the samples were investigate by SEM, EDS, XRD and XPS. The results indicated that the corrosion degree was accelerated with increasing POM ? The intense localised corrosion occurred on the surface of samples. The corrosion films mainly comprised of FeCO3 and complex phase products (Fe, Ca....)COj. There exists serious pitting on the metal substrates under the corrosion film. The theoretic and experimental analyses indicate this is caused by existed micropores or micro holes in films, which have the function of mass transportation. | LIChun-fu DAIJia-lin WANGBing ZHANGYing ZHANGXian-ju LUDong-li LUOPing-ya | 2004 | 材料热处理学报2004，25，05B： | 2 |
| 17 | Genome-wide identification, evolution, and expression analysis of RNA-binding glycine-rich protein family in maize显示文摘The RNA‐binding glycine‐rich protein(RB‐GRP)family is characterized by the presence of a glycine‐rich domain arranged in(Gly)n‐X repeats and an RNA‐recognition motif(RRM). RB‐GRPs participate in varied physiological and biochemical processes especially in the stress response of plants. In this study, a total of 23 RB‐GRPs distributed on 10 chromosomes were identified in maize(Zea mays L.), and they were divided into four subgroups according to their conserved domain architecture. Five pairs of paralogs were identified,while none of them was located on the same chromosomal region, suggesting that segmental duplication is predominant in the duplication events of the RB‐GRPs in maize. Comparative analysis of RB‐GRPs in maize, Arabidopsis(Arabidopsis thaliana L.), rice(Oryza sativa L.), and wheat(Triticum aestivum)revealed that two exclusive subgroups were only identified in maize. Expression of eight ZmRB‐GRPs was significantly regulated by at least two kinds of stresses. In addition, cis‐elements predicted in the promoter regions of the ZmRB‐GRPs also indicated that these ZmRB‐GRPs would be involved in stress response of maize. The preliminary genome‐wide analysis of the RB‐GRPs in maize would provide useful information for further study on the function of the ZmRB‐GRPs. | Jianhua Zhangy Yanxin Zhaoy Hailin Xiao Yonglian Zheng Bing Yue | 2014 | Journal of Integrative Plant Biology2014，56，10： | 2 |
| 18 | Genome sequencing and characterization analysis of a Beijing isolate of chicken coronavirus infectious bronchitis virus显示文摘Avian infectious bronchitis virus (AIBV) is classified as a member of the genus coronavirus in the family coronaviridae. The enveloped virus has a positive-sense, sin-gle-stranded RNA genome of approximately 28 kilo-bases, which has a 5′ cap structure and 3′ polyadenylation tract. The complete genome sequence of infectious bronchitis virus (IBV), Beijing isolate, was determined by cloning sequencing and primer walking. The whole genome is 27733 nucleotides in length, has ten open reading frames: 5′-orf1a-orf1ab-s-3a- 3b-e-m- 6a-6b-n-3′. Alignments of the genome sequence of IBV Beijing isolate with those of two AIBV strains and one SARS coronavirus were performed respectively. The genome sequence of IBV Beijing isolate compared with that of the IBV strain LX4 (uncompleted, 19440 bp in size) was 91.2% similarity. However, the full-length genome sequence of IBV Beijing isolate was 85.2% identity to that of IBV Strain Beaudette, and was only 50.8% homology to that of SARS coronavirus. The results showed that the genome of IBV has remarkable variation. And IBV Beijing isolate is not closely related to SARS coronavirus. Phylogenetic analyses based on the whole genome sequence, S protein, M protein and N pro-tein, also showed that AIBV Beijing isolate is lone virus in group Ⅲ and is distant from SARS coronavirus. In conclu-sion, this study will contribute to the studies of diagnosis and diseases control on IBV in China. | JINWeiwu CHENChen ZHANGYing ZHAOYiqiang FENGJidong CHENFuyong WUQingming YANGHanchun WANGMing YUJialin LINing GONGYuanshi SUNQixin CHENZhangliang | 2004 | Chinese Science Bulletin2004，49，6： | 2 |
| 19 | Nuclear translocation of OsMFT1 that is impeded by OsFTIP1 promotes drought toleranee in rice显示文摘Drought is the leading environmental threat affecting crop productivity,and plants have evolved a series of mechanisms to adapt to drought stress.The FT-interacting proteins(FTIPs)and phosphatidylethanola mine.binding proteins(PEBPs)play key roles in developmental processes,whereas their roles in the regulation of stress response are still largely unknown.Here,we report that OsFTIP1 negatively regulates drought response in rice.We showed that OsFTIP1 interacts with rice MOTHER OF FT AND TFL1(OsMFT1),a PEBP that promotes rice tolerance to drought treatment.Further studies discovered that OsMFT1 interacts with two key drought-related transcription factors,OsbZIP66 and OsMYB26,regulating their binding capacity on drought-related genes and thereby enhancing drought toleranee in rice.Interestingly,we found that OsFTIP1 impedes the nucleocytoplasmic translocation of OsMFT1,implying that dynamic modulation of drought-responsive genes by the OsMFT1-OsMYB26 and OsMFT1-OsbZIP66 complexes is integral to OsFTIP1-modulated nuclear accumulation of OsMFT1.Our findings also suggest that OsMFT1 might act as a hitherto unknown nucleocytoplasmic trafficking signal that regulates drought tolerance in rice in response to environmental signals. | Ying Chen Jun Shen Liang Zhangi Haoyue Qi Lijia Yang Huanyu Wang Jiaxuan Wang Yuexing Wang Hao Du Zeng Tao Ting Zhao Pingchuan Deng Qingyao Shu Qian Qian Hao Yu Shiyong Song | 2021 | Molecular Plant2021，14，8： | 2 |
| 20 | Remifentanil preconditioning confers cardio protection via cardiac kappa-and delta-opioid receptors 显示文摘 | ZhangY Irwin MG Wong TM | 2005 | Anesthesiology2005，102，2： | 1 |
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